Rw1G027930

Endoplasmic reticulum metallopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0011223
Physical Location & Seq
Reverse (-)
55427458 .. 55434233
6776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027930.1

Sequence Viewer

Length: 2685 bp
ATGGCCCGTGCTAAGTCCATGCTGTCAATCAAGTCATACATGGTAGATCAGGCAGGGAATATCTCCCGAACCACAACCAGAACAAGAACCAACTCAAACCCAAGTGTCACCACCCCATGGGTTGTTCAGAGGCCACAGCGATCACCCTTTGTCTGGCTCACCTTGTTGGCAGCCATCACATACTCGTGTTGGGGCGTTTATCATTACCAGTTCGAGTCCCTCCCCGCCCCTCTCACTCCTGACCAGGCCGGAAAGCGAGGCTTCTCCGAGTTCTCTGCTCGGAAACACGTCAAGGTTCTGACCCAGTTGGGCCCTCACCCTGTTGCCTCTGAAGCCATCACTCTTGCTCAGCAGTATGTATTGGCAGAAGCAGAGGAGATTAAGAAAACGGCTCACTGGGAAGTTGATGTGGAGGTGGACTCGTTTGTTGCGAAAACCGGAGCTAATCAAATGAAGAGTGGATTATTTAAGGGGAAAACACTTGTGTATTCGGATTTGAATCACATTGTTGTAAGAATCTCGCCCAAGTATTCACCTATTTCTGTGGACAATGCTGTTCTCGTGTCTTCTCACATTGACACTGTCTTCTCAACAGGAGGGGCCGGAGATTGCAGTTCGTGTGTGGCTGTTATGTTGGAACTTGCTCGTGGAGTTTCGCAGTGGGCGCATGGATTTAAGCATGCTGTTATATTTTTGTTTAACACTGGGGAGGAGGAAGGTCTAGATGGTGCTCATAGCTTTATAACTCAGCATCCATGGAGCAGTACGATTCGTCTGGCTATTGATTTGGAGGCCATGGGTATTGGAGGGAAGTCTGGCATATTTCAGGCCGGTCCTCATCCTTGGGCTATTGAGAATTATGCTGCAGTAGCGAAATACCCATCTGGCCACATAATAGGACAGGTTTCCATCAAATCTGCAACAGATTTCCAAGTATACAAAGAGTTAGCTGGCCTTTCAGGCCTTGACTTTGCTTACTCAGATAACAGTGCAGTATACCACACGAAGAATGACAAATTCGAGCTTCTTCAATTGGGATCTCTTCAACATCTTGGAGAAAATATGCTTGCTTTTCTGCTCCGGTCGCTGCATCCTCTCATCTTCCAAAAGCCAGGGATGTACATGATTGTCTACCGTCAACGCTTCGCCAGAATGCTTCATAATTCAGTGATAGCGCAATCACTTCTTATATGGACTACATCATTGCTTATGGGTGGATATCCAGCTGCAGTATCATTGGTCTTGTCATGTTTAAGTGTCATTCTTATGTGGACATTTGCATTAAGTTTCTCTGTTATTGTGGCCTTTATTATACCCCTAATATCATCATCACCAGTGCCATATGTCGCAAACCCATGGCTTGTAGTTGGGTTATTCGCTGCACCTGCTCTGCTTGGAGCATTAACTGGTCAATATTTGGGTTATCTTGTTCTTCATACGTATTTGGCAAACGCTTACTCCAAGAAAAAACAGCTGTCACCTGCTATCCGAGCCGATTTGGTTAAATTAGAGGCTGAAAGATGGCTCTACAAAGCTGGATCAATTCAATGGCTCATTCTCCTCTGTTTGGGAACCTATTACAGAATTGGATCGTCTTATCTGGCTCTTGCTTGGTTGGTTCCACCAGCATTCGCATATGGCTTTCTTGAAGCAACTCTAAGCCCAGTCCGATTACCTAAGCCACTGAAACTTGCAACTCTGCTGATAGGATTAGCTGTACCGATAGTACTTTCTGCTGGCGTATTCATTAGGGTGGCTGGCACAATAATCGGGACCATGGTTCGTTTTGATAGGAATCCTGGTGGCACTCCTGATTGGCTGGGAAATGTTATACTTGCTGTTTTTGTTTCTGCTGTCATGTGCCTGACTTTGGTGTATCTTCTTTCATACATTCATCTTTCAGGTGCCAAGAGATTGATTGTTCTCTCAACTTGCGCCTTGTTTTGCCTCTCGCTTGCTCTTGTATTATCTGGTACTGTACCAGCATTTACCAAAGACACATCAAGAGCTGTAAATGTTGTGCATGTTGTAGATACTACAAGAAGCATTGAAGATCCTAGATCCTATGTGTCTCTGTTTTCTTCAACTCCTGGAAAATTGACAAAGGAGGTTGAGCTAATTAATGAAGGTTTCAGATGTGGTCGAGATCAGGTCTTTGATTTTGTAACTTTTACAGTGAAATACGGCTGTTGGACTGAGGATGACAGTGACAATGGGTGGAGTGAAGCAGACATTCCCATAATGCATGTTCAGAGTGATACTCAAGGTATTGAAAGAACAACACAAGTTTTAATTGATACGAAGGGTTCATTACGGTGGTCTCTTGCAATAAACATGGATGAAATACGGGATTTTGCATTCACAGATGCTGGAAACTCTGAGGAATTGGTTTCAGTTGGTGACAAGAGCGCTGTAGATGGTTGGCATGTTATTCAGTTTGCCGGGGGAAACAATTCACCAACGAAGTTTGGTCTGACTGTCTTCTGGACCAAAAACTCTACCCTTAAGGCGGACGGGAAGAGAGACGAGACAGCTCCCCTTCTCAAGTTAAGAACTGATATGGACATTGTAACACCGAAAGTCGAAAGAGTTCTTTCAAAGCTTCCTACTTGGTGTTCACTGTTTGGCAAGTCCACGTCTCCCTACACCTTTGCATTCTTAAGTAGTCTTCCTGTTGATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M28

Protein Analysis

894

Amino Acids

98.06

Weight (kDa)

8.52

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M28 PF04389 169 - 358 1.8e-41 Peptidase family M28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20660 AT5G20660 AT5G20660 AT5G20660
fragaria_vesca FvH4_7g19460 FvH4_7g19460 FvH4_7g19460 FvH4_7g19460
malus_domestica MD07G1174500.v1.1
prunus_persica Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1
pyrus_communis pycom07g16870
rosa_chinensis RchiOBHm_Chr1g0363331
rosa_laevigata RLG00000027573
rosa_roxburghii Rroxscaffold_4G00293270
rosa_rugosa Rorug01G0306400
rosa_samantha Rh1AG315200 Rh1CG294900
rosa_wichuraiana Rw1G027930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
AarI CACCTGC 2 cut(s) 1393, 1489
Acc36I ACCTGC 2 cut(s) 1393, 1489
AccB1I GGYRCC 1 cut(s) 1904
AccB7I CCANNNNNTGG 1 cut(s) 117
AccI GTMKAC 3 cut(s) 936, 996, 1131
AciI CCGC 2 cut(s) 225, 2510
AclWI GGATC 5 cut(s) 1045, 1546, 1597, 2048, 2055
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 1 cut(s) 1016
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 6 cut(s) 766, 1121, 1719, 1728, 1975, 1980
AfeI AGCGCT 1 cut(s) 2410
AfiI CCNNNNNNNGG 5 cut(s) 117, 153, 1567, 1870, 2509
AflII CTTAAG 2 cut(s) 2504, 2659
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 9 cut(s) 499, 1031, 1046, 1547, 1649, 2051, 2085, 2273, 2598
AjiI CACGTC 2 cut(s) 289, 2637
AjnI CCWGG 4 cut(s) 243, 1111, 1798, 2089
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 5 cut(s) 2076, 2325, 2517, 2522, 2643
AlwI GGATC 5 cut(s) 1045, 1546, 1597, 2048, 2055
AlwNI CAGNNNCTG 1 cut(s) 2369
Aor51HI AGCGCT 1 cut(s) 2410
ApaI GGGCCC 1 cut(s) 314
ApeKI GCWGC 5 cut(s) 170, 863, 1087, 1226, 1379
ApoI RAATTY 1 cut(s) 1016
AseI ATTAAT 1 cut(s) 2121
Asp700I GAANNNNTTC 2 cut(s) 2452, 2589
AspLEI GCGC 4 cut(s) 667, 1177, 1937, 2411
AspS9I GGNCC 7 cut(s) 4, 310, 311, 600, 833, 1773, 2487
AsuC2I CCSGG 1 cut(s) 2443
AsuHPI GGTGA 9 cut(s) 100, 135, 151, 308, 525, 1323, 1470, 2411, 2448
AvaII GGWCC 3 cut(s) 833, 1773, 2487
BaeGI GKGCMC 1 cut(s) 314
BalI TGGCCA 1 cut(s) 888
BanI GGYRCC 1 cut(s) 1904
BanII GRGCYC 1 cut(s) 314
BauI CACGAG 3 cut(s) 184, 560, 645
BbsI GAAGAC 4 cut(s) 558, 577, 2473, 2660
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 5 cut(s) 182, 850, 1074, 1213, 1366
BccI CCATC 7 cut(s) 182, 344, 719, 889, 917, 1515, 2411
BceAI ACGGC 2 cut(s) 405, 2200
BcgI CGANNNNNNTGC 4 cut(s) 257, 291, 1750, 1784
BciT130I CCWGG 4 cut(s) 245, 1113, 1800, 2091
BcnI CCSGG 1 cut(s) 2443
BcoDI GTCTC 5 cut(s) 2076, 2325, 2517, 2522, 2643
BfaI CTAG 2 cut(s) 722, 2058
BfmI CTRYAG 3 cut(s) 864, 1227, 2412
BfoI RGCGCY 1 cut(s) 2412
BfrI CTTAAG 2 cut(s) 2504, 2659
BfuAI ACCTGC 2 cut(s) 1393, 1489
BglI GCCNNNNNGGC 1 cut(s) 960
BisI GCNGC 5 cut(s) 171, 864, 1088, 1227, 1380
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 5 cut(s) 172, 865, 1089, 1228, 1381
BmcAI AGTACT 1 cut(s) 1728
Bme1390I CCNGG 5 cut(s) 245, 1113, 1800, 2091, 2443
Bme18I GGWCC 3 cut(s) 833, 1773, 2487
BmgBI CACGTC 2 cut(s) 289, 2637
BmgT120I GGNCC 7 cut(s) 4, 310, 311, 600, 833, 1773, 2487
BmiI GGNNCC 6 cut(s) 312, 601, 1573, 1620, 1774, 1906
BmrFI CCNGG 5 cut(s) 245, 1113, 1800, 2091, 2443
BmrI ACTGGG 4 cut(s) 298, 406, 714, 1658
BmsI GCATC 3 cut(s) 760, 1099, 2356
BmuI ACTGGG 4 cut(s) 298, 406, 714, 1658
BpiI GAAGAC 4 cut(s) 558, 577, 2473, 2660
BplI GAGNNNNNCTC 4 cut(s) 404, 436, 2245, 2277
Bpu10I CCTNAGC 1 cut(s) 1677
Bpu1102I GCTNAGC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 2247, 2528
BpuMI CCSGG 1 cut(s) 2443
BsaAI YACGTR 1 cut(s) 1440
BsaBI GATNNNNATC 2 cut(s) 498, 1794
BsaI GGTCTC 1 cut(s) 2325
BsaJI CCNNGG 8 cut(s) 116, 755, 795, 842, 1112, 1355, 1776, 2442
BsaWI WCCGGW 2 cut(s) 437, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 1442, 1472
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 153, 1567, 1870, 2509
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 7 cut(s) 208, 304, 401, 709, 1334, 1411, 1664
Bse3DI GCAATG 1 cut(s) 1202
Bse8I GATNNNNATC 2 cut(s) 498, 1794
BseBI CCWGG 4 cut(s) 245, 1113, 1800, 2091
BseDI CCNNGG 8 cut(s) 116, 755, 795, 842, 1112, 1355, 1776, 2442
BseGI GGATG 6 cut(s) 751, 838, 1090, 1122, 2206, 2344
BseJI GATNNNNATC 2 cut(s) 498, 1794
BseLI CCNNNNNNNGG 5 cut(s) 117, 153, 1567, 1870, 2509
BseMI GCAATG 1 cut(s) 1202
BseMII CTCAG 5 cut(s) 362, 761, 993, 2187, 2370
BseNI ACTGG 7 cut(s) 208, 304, 401, 709, 1334, 1411, 1664
BseRI GAGGAG 3 cut(s) 389, 725, 1550
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 5 cut(s) 182, 850, 1074, 1213, 1366
BseYI CCCAGC 1 cut(s) 1819
BsgI GTGCAG 2 cut(s) 1011, 1365
Bsh1285I CGRYCG 1 cut(s) 1085
BshNI GGYRCC 1 cut(s) 1904
BsiEI CGRYCG 1 cut(s) 1085
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 6 cut(s) 249, 438, 603, 831, 1081, 2442
BslFI GGGAC 2 cut(s) 202, 1786
BslI CCNNNNNNNGG 5 cut(s) 117, 153, 1567, 1870, 2509
BsmAI GTCTC 5 cut(s) 2076, 2325, 2517, 2522, 2643
BsmBI CGTCTC 2 cut(s) 2517, 2643
BsmFI GGGAC 2 cut(s) 202, 1786
BsmI GAATGC 4 cut(s) 1158, 1628, 2357, 2654
Bso31I GGTCTC 1 cut(s) 2325
Bsp120I GGGCCC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 314, 733
Bsp1407I TGTACA 1 cut(s) 1119
Bsp143I GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
Bsp1720I GCTNAGC 1 cut(s) 348
Bsp19I CCATGG 5 cut(s) 116, 755, 795, 1355, 1776
BspACI CCGC 2 cut(s) 225, 2510
BspCNI CTCAG 5 cut(s) 361, 760, 992, 2188, 2371
BspLI GGNNCC 6 cut(s) 312, 601, 1573, 1620, 1774, 1906
BspMAI CTGCAG 2 cut(s) 868, 1231
BspMI ACCTGC 2 cut(s) 1393, 1489
BspPI GGATC 5 cut(s) 1045, 1546, 1597, 2048, 2055
BspT107I GGYRCC 1 cut(s) 1904
BspTI CTTAAG 2 cut(s) 2504, 2659
BspTNI GGTCTC 1 cut(s) 2325
BsrDI GCAATG 1 cut(s) 1202
BsrFI RCCGGY 1 cut(s) 830
BsrGI TGTACA 1 cut(s) 1119
BsrI ACTGG 7 cut(s) 208, 304, 401, 709, 1334, 1411, 1664
BssAI RCCGGY 1 cut(s) 830
BssECI CCNNGG 8 cut(s) 116, 755, 795, 842, 1112, 1355, 1776, 2442
BssMI GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
BssNAI GTATAC 2 cut(s) 937, 997
BssSI CACGAG 3 cut(s) 184, 560, 645
BssT1I CCWWGG 6 cut(s) 116, 755, 795, 842, 1355, 1776
Bst1107I GTATAC 2 cut(s) 937, 997
Bst2BI CACGAG 3 cut(s) 184, 560, 645
Bst2UI CCWGG 4 cut(s) 245, 1113, 1800, 2091
Bst4CI ACNGT 9 cut(s) 583, 989, 1136, 1978, 2176, 2207, 2316, 2479, 2622
Bst6I CTCTTC 3 cut(s) 449, 1047, 2513
BstAFI CTTAAG 2 cut(s) 2504, 2659
BstAUI TGTACA 1 cut(s) 1119
BstBAI YACGTR 1 cut(s) 1440
BstC8I GCNNGC 6 cut(s) 681, 952, 1068, 1738, 1759, 1956
BstDEI CTNAG 8 cut(s) 12, 348, 747, 979, 1658, 1677, 2196, 2379
BstDSI CCRYGG 5 cut(s) 116, 755, 795, 1355, 1776
BstF5I GGATG 6 cut(s) 751, 838, 1090, 1122, 2206, 2344
BstH2I RGCGCY 1 cut(s) 2412
BstHHI GCGC 4 cut(s) 667, 1177, 1937, 2411
BstKTI GATC 8 cut(s) 49, 143, 1040, 1541, 1592, 2056, 2063, 2149
BstMAI GTCTC 5 cut(s) 2076, 2325, 2517, 2522, 2643
BstMBI GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
BstMCI CGRYCG 1 cut(s) 1085
BstMWI GCNNNNNNNGC 7 cut(s) 332, 664, 869, 960, 1084, 1385, 1490
BstNI CCWGG 4 cut(s) 245, 1113, 1800, 2091
BstNSI RCATGY 4 cut(s) 683, 2027, 2249, 2429
BstSCI CCNGG 5 cut(s) 243, 1111, 1798, 2089, 2441
BstSFI CTRYAG 3 cut(s) 864, 1227, 2412
BstSLI GKGCMC 1 cut(s) 314
BstSNI TACGTA 1 cut(s) 1440
BstV1I GCAGC 5 cut(s) 182, 850, 1074, 1213, 1366
BstV2I GAAGAC 4 cut(s) 558, 577, 2473, 2660
BstX2I RGATCY 3 cut(s) 1037, 2053, 2060
BstYI RGATCY 3 cut(s) 1037, 2053, 2060
BstZ17I GTATAC 2 cut(s) 937, 997
BtgI CCRYGG 5 cut(s) 116, 755, 795, 1355, 1776
BtrI CACGTC 2 cut(s) 289, 2637
BtsCI GGATG 6 cut(s) 751, 838, 1090, 1122, 2206, 2344
BtsI GCAGTG 1 cut(s) 665
BveI ACCTGC 2 cut(s) 1393, 1489
Cac8I GCNNGC 6 cut(s) 681, 952, 1068, 1738, 1759, 1956
CaiI CAGNNNCTG 1 cut(s) 2369
CfoI GCGC 4 cut(s) 667, 1177, 1937, 2411
Cfr10I RCCGGY 1 cut(s) 830
Cfr13I GGNCC 7 cut(s) 4, 310, 311, 600, 833, 1773, 2487
Csp6I GTAC 6 cut(s) 765, 1120, 1718, 1727, 1974, 1979
CviQI GTAC 6 cut(s) 765, 1120, 1718, 1727, 1974, 1979
DdeI CTNAG 8 cut(s) 12, 348, 747, 979, 1658, 1677, 2196, 2379
DpnI GATC 8 cut(s) 48, 142, 1039, 1540, 1591, 2055, 2062, 2148
DpnII GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 3 cut(s) 449, 1047, 2513
EarI CTCTTC 3 cut(s) 449, 1047, 2513
EciI GGCGGA 1 cut(s) 2525
Eco105I TACGTA 1 cut(s) 1440
Eco130I CCWWGG 6 cut(s) 116, 755, 795, 842, 1355, 1776
Eco147I AGGCCT 1 cut(s) 963
Eco24I GRGCYC 1 cut(s) 314
Eco31I GGTCTC 1 cut(s) 2325
Eco32I GATATC 1 cut(s) 1220
Eco47I GGWCC 3 cut(s) 833, 1773, 2487
Eco47III AGCGCT 1 cut(s) 2410
Eco57I CTGAAG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 311
EcoRII CCWGG 4 cut(s) 243, 1111, 1798, 2089
EcoRV GATATC 1 cut(s) 1220
EcoT14I CCWWGG 6 cut(s) 116, 755, 795, 842, 1355, 1776
EcoT22I ATGCAT 1 cut(s) 2247
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 6 cut(s) 116, 755, 795, 842, 1355, 1776
Esp3I CGTCTC 2 cut(s) 2517, 2643
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 2 cut(s) 202, 1786
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 2 cut(s) 1342, 1636
FblI GTMKAC 3 cut(s) 936, 996, 1131
Fnu4HI GCNGC 5 cut(s) 171, 864, 1088, 1227, 1380
FokI GGATG 6 cut(s) 738, 825, 1077, 1129, 2213, 2351
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 5 cut(s) 171, 864, 1088, 1227, 1380
FspBI CTAG 2 cut(s) 722, 2058
GlaI GCGC 4 cut(s) 666, 1176, 1936, 2410
GluI GCNGC 5 cut(s) 171, 864, 1088, 1227, 1380
GsaI CCCAGC 1 cut(s) 1823
HaeII RGCGCY 1 cut(s) 2412
HapII CCGG 6 cut(s) 249, 438, 603, 831, 1081, 2442
HhaI GCGC 4 cut(s) 667, 1177, 1937, 2411
Hin6I GCGC 4 cut(s) 665, 1175, 1935, 2409
HinP1I GCGC 4 cut(s) 665, 1175, 1935, 2409
HincII GTYRAC 1 cut(s) 1139
HindII GTYRAC 1 cut(s) 1139
HindIII AAGCTT 1 cut(s) 2600
HinfI GANTC 6 cut(s) 215, 419, 499, 516, 769, 1795
HpaII CCGG 6 cut(s) 249, 438, 603, 831, 1081, 2442
HphI GGTGA 9 cut(s) 100, 135, 151, 308, 525, 1323, 1470, 2411, 2448
Hpy166II GTNNAC 9 cut(s) 418, 547, 937, 997, 1132, 1139, 1272, 2618, 2634
Hpy188III TCNNGA 9 cut(s) 66, 239, 722, 1646, 1771, 1811, 2004, 2144, 2485
Hpy8I GTNNAC 9 cut(s) 418, 547, 937, 997, 1132, 1139, 1272, 2618, 2634
HpyAV CCTTC 4 cut(s) 710, 2120, 2296, 2549
HpyCH4III ACNGT 9 cut(s) 583, 989, 1136, 1978, 2176, 2207, 2316, 2479, 2622
HpyCH4IV ACGT 3 cut(s) 288, 1439, 2636
HpyF10VI GCNNNNNNNGC 7 cut(s) 332, 664, 869, 960, 1084, 1385, 1490
HpyF3I CTNAG 8 cut(s) 12, 348, 747, 979, 1658, 1677, 2196, 2379
HpySE526I ACGT 3 cut(s) 288, 1439, 2636
HspAI GCGC 4 cut(s) 665, 1175, 1935, 2409
Kzo9I GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
LmnI GCTCC 5 cut(s) 440, 759, 1083, 1397, 2539
Lsp1109I GCAGC 5 cut(s) 182, 850, 1074, 1213, 1366
LweI GCATC 3 cut(s) 760, 1099, 2356
MaeI CTAG 2 cut(s) 722, 2058
MaeII ACGT 3 cut(s) 288, 1439, 2636
MaeIII GTNAC 6 cut(s) 106, 1476, 2164, 2207, 2399, 2569
MalI GATC 8 cut(s) 48, 142, 1039, 1540, 1591, 2055, 2062, 2148
MboI GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
MfeI CAATTG 1 cut(s) 1031
MflI RGATCY 3 cut(s) 1037, 2053, 2060
MhlI GDGCHC 2 cut(s) 314, 733
MlsI TGGCCA 1 cut(s) 888
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 2 cut(s) 224, 413
MmeI TCCRAC 2 cut(s) 615, 2171
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 1 cut(s) 2247
MroXI GAANNNNTTC 2 cut(s) 2452, 2589
MscI TGGCCA 1 cut(s) 888
MslI CAYNNNNRTG 4 cut(s) 184, 1265, 1751, 2314
Msp20I TGGCCA 1 cut(s) 888
MspA1I CMGCKG 2 cut(s) 1226, 1474
MspCI CTTAAG 2 cut(s) 2504, 2659
MspI CCGG 6 cut(s) 249, 438, 603, 831, 1081, 2442
MspR9I CCNGG 5 cut(s) 245, 1113, 1800, 2091, 2443
MunI CAATTG 1 cut(s) 1031
Mva1269I GAATGC 4 cut(s) 1158, 1628, 2357, 2654
MvaI CCWGG 4 cut(s) 245, 1113, 1800, 2091
MwoI GCNNNNNNNGC 7 cut(s) 332, 664, 869, 960, 1084, 1385, 1490
NciI CCSGG 1 cut(s) 2443
NcoI CCATGG 5 cut(s) 116, 755, 795, 1355, 1776
NdeI CATATG 2 cut(s) 1342, 1636
NdeII GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
NlaIV GGNNCC 6 cut(s) 312, 601, 1573, 1620, 1774, 1906
NmuCI GTSAC 4 cut(s) 106, 1476, 2207, 2399
NsiI ATGCAT 1 cut(s) 2247
NspI RCATGY 4 cut(s) 683, 2027, 2249, 2429
PaeI GCATGC 1 cut(s) 683
PaqCI CACCTGC 2 cut(s) 1393, 1489
PceI AGGCCT 1 cut(s) 963
PcsI WCGNNNNNNNCGW 1 cut(s) 428
PctI GAATGC 4 cut(s) 1158, 1628, 2357, 2654
PdmI GAANNNNTTC 2 cut(s) 2452, 2589
PfeI GAWTC 4 cut(s) 499, 516, 769, 1795
PflFI GACNNNGTC 1 cut(s) 581
PflMI CCANNNNNTGG 1 cut(s) 117
PfoI TCCNGGA 1 cut(s) 2089
PkrI GCNGC 5 cut(s) 172, 865, 1089, 1228, 1381
PleI GAGTC 2 cut(s) 223, 413
PpsI GAGTC 2 cut(s) 223, 413
Ppu21I YACGTR 1 cut(s) 1440
PshBI ATTAAT 1 cut(s) 2121
PsiI TTATAA 1 cut(s) 743
Psp6I CCWGG 4 cut(s) 243, 1111, 1798, 2089
PspFI CCCAGC 1 cut(s) 1819
PspGI CCWGG 4 cut(s) 243, 1111, 1798, 2089
PspN4I GGNNCC 6 cut(s) 312, 601, 1573, 1620, 1774, 1906
PspOMI GGGCCC 1 cut(s) 310
PspPI GGNCC 7 cut(s) 4, 310, 311, 600, 833, 1773, 2487
PstI CTGCAG 2 cut(s) 868, 1231
PstNI CAGNNNCTG 1 cut(s) 2369
PsuI RGATCY 3 cut(s) 1037, 2053, 2060
PsyI GACNNNGTC 1 cut(s) 581
PvuII CAGCTG 2 cut(s) 1226, 1474
RsaI GTAC 6 cut(s) 766, 1121, 1719, 1728, 1975, 1980
RsaNI GTAC 6 cut(s) 765, 1120, 1718, 1727, 1974, 1979
RseI CAYNNNNRTG 4 cut(s) 184, 1265, 1751, 2314
SatI GCNGC 5 cut(s) 171, 864, 1088, 1227, 1380
Sau3AI GATC 8 cut(s) 46, 140, 1037, 1538, 1589, 2053, 2060, 2146
Sau96I GGNCC 7 cut(s) 4, 310, 311, 600, 833, 1773, 2487
ScaI AGTACT 1 cut(s) 1728
SchI GAGTC 2 cut(s) 224, 413
ScrFI CCNGG 5 cut(s) 245, 1113, 1800, 2091, 2443
SduI GDGCHC 2 cut(s) 314, 733
SfaNI GCATC 3 cut(s) 760, 1099, 2356
SfcI CTRYAG 3 cut(s) 864, 1227, 2412
SfiI GGCCNNNNNGGCC 1 cut(s) 960
SinI GGWCC 3 cut(s) 833, 1773, 2487
SmiMI CAYNNNNRTG 4 cut(s) 184, 1265, 1751, 2314
SmlI CTYRAG 4 cut(s) 2262, 2504, 2543, 2659
SmoI CTYRAG 4 cut(s) 2262, 2504, 2543, 2659
SnaBI TACGTA 1 cut(s) 1440
SphI GCATGC 1 cut(s) 683
SseBI AGGCCT 1 cut(s) 963
SsiI CCGC 2 cut(s) 225, 2510
SspI AATATT 1 cut(s) 1415
SspMI CTAG 2 cut(s) 722, 2058
StuI AGGCCT 1 cut(s) 963
StyD4I CCNGG 5 cut(s) 243, 1111, 1798, 2089, 2441
StyI CCWWGG 6 cut(s) 116, 755, 795, 842, 1355, 1776
TaaI ACNGT 9 cut(s) 583, 989, 1136, 1978, 2176, 2207, 2316, 2479, 2622
TaiI ACGT 3 cut(s) 291, 1442, 2639
TaqI TCGA 4 cut(s) 213, 1020, 2143, 2583
TatI WGTACW 2 cut(s) 1119, 1726
TfiI GAWTC 4 cut(s) 499, 516, 769, 1795
TseFI GTSAC 4 cut(s) 106, 1476, 2207, 2399
TseI GCWGC 5 cut(s) 170, 863, 1087, 1226, 1379
Tsp45I GTSAC 4 cut(s) 106, 1476, 2207, 2399
TspDTI ATGAA 9 cut(s) 467, 1148, 1424, 1735, 1875, 1883, 2139, 2298, 2355
Tth111I GACNNNGTC 1 cut(s) 581
Van91I CCANNNNNTGG 1 cut(s) 117
Vha464I CTTAAG 2 cut(s) 2504, 2659
VpaK11BI GGWCC 3 cut(s) 833, 1773, 2487
VspI ATTAAT 1 cut(s) 2121
XapI RAATTY 1 cut(s) 1016
XbaI TCTAGA 1 cut(s) 721
XceI RCATGY 4 cut(s) 683, 2027, 2249, 2429
XmiI GTMKAC 3 cut(s) 936, 996, 1131
XmnI GAANNNNTTC 2 cut(s) 2452, 2589
XspI CTAG 2 cut(s) 722, 2058
ZrmI AGTACT 1 cut(s) 1728
Zsp2I ATGCAT 1 cut(s) 2247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.