FvH4_7g22230

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0017713
Physical Location & Seq
Forward (+)
17564364 .. 17565899
1536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22230.t1

Sequence Viewer

Length: 531 bp
ATGGCCGCAATGCTTCCGCCTCCGGTGGCGCGAATTGTTATTCTTGTATTGAGGATCCTGATTACCATTACACTGTTTGCATCATTCATCATTGTCGTCACCAATAGCCAGGATTATTATGACCTTGGCCTCCACCAGAGGAGAACCTGGCATTTCACTGATGAAATTGGACTTCAATATATGGCTGCCACATTAGGTCTCGGAATCGTGTTTTCAATATATGGAACGGCAGTCATGGCTTTGCGCATCAAGAGAGGAAATGATGAAGGGAACTTGTTGATTGATTTCTATGGCCAGAAGGTTTTGTCGAACTTATTAGTTACAGCAACTGTTGCCGCATTTCTTACGATCCAGAAGTTTTATAAAGAAAGGGAAGATAACTTGAAGATATTTCCGCAGAGTGATCTTGGGACTTTTGAGTATTATTGGGGGAGGTATAGGACATCTTCTGGACTTGTCATCCTTGCATTCTTTTTCTCGTTTGCACTGTCGGTTCTGTCCACCCATAGACTTGCTAGCAGGATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

177

Amino Acids

19.99

Weight (kDa)

9.22

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 11 - 121 7.8e-10 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017713)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g22230
rosa_chinensis RchiOBHm_Chr1g0366621
rosa_laevigata RLG00000027332
rosa_multiflora Rmu_sc0003180.1_g000008
rosa_roxburghii Rroxscaffold_4G00289810
rosa_rugosa Rorug01G0330900 Rorug01G0331000
rosa_samantha Rh1AG339300 Rh1BG301000 Rh1CG315800 Rh1DG332600
rosa_wichuraiana Rw1G030050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 363
Acc16I TGCGCA 1 cut(s) 245
AccII CGCG 1 cut(s) 31
AciI CCGC 4 cut(s) 6, 17, 336, 395
AclWI GGATC 3 cut(s) 49, 62, 343
AcoI YGGCCR 2 cut(s) 3, 292
AgsI TTSAA 4 cut(s) 176, 216, 385, 527
AjnI CCWGG 2 cut(s) 108, 146
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 3 cut(s) 49, 62, 343
AlwNI CAGNNNCTG 1 cut(s) 329
AoxI GGCC 3 cut(s) 3, 127, 292
ApeKI GCWGC 1 cut(s) 185
AspLEI GCGC 2 cut(s) 31, 246
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 515
BalI TGGCCA 1 cut(s) 294
BamHI GGATCC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 172
BceAI ACGGC 1 cut(s) 243
BciT130I CCWGG 2 cut(s) 110, 148
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 1 cut(s) 516
BisI GCNGC 3 cut(s) 6, 186, 336
BlsI GCNGC 3 cut(s) 7, 187, 337
Bme1390I CCNGG 2 cut(s) 110, 148
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 2 cut(s) 110, 148
BmsI GCATC 2 cut(s) 89, 255
BmtI GCTAGC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 124
BsaWI WCCGGW 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 2 cut(s) 110, 148
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 1 cut(s) 459
BseMI GCAATG 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 154
BseXI GCAGC 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 31
BshFI GGCC 3 cut(s) 5, 129, 294
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 424
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 424
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 3 cut(s) 5, 129, 294
Bso31I GGTCTC 1 cut(s) 203
Bsp143I GATC 3 cut(s) 54, 348, 403
BspACI CCGC 4 cut(s) 6, 17, 336, 395
BspANI GGCC 3 cut(s) 5, 129, 294
BspFNI CGCG 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 56
BspOI GCTAGC 1 cut(s) 519
BspPI GGATC 3 cut(s) 49, 62, 343
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 3 cut(s) 54, 348, 403
BssT1I CCWWGG 1 cut(s) 124
Bst2UI CCWGG 2 cut(s) 110, 148
Bst4CI ACNGT 3 cut(s) 75, 331, 489
BstAPI GCANNNNNTGC 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 517
BstF5I GGATG 1 cut(s) 459
BstFNI CGCG 1 cut(s) 31
BstHHI GCGC 2 cut(s) 31, 246
BstKTI GATC 3 cut(s) 57, 351, 406
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 3 cut(s) 54, 348, 403
BstMWI GCNNNNNNNGC 2 cut(s) 236, 332
BstNI CCWGG 2 cut(s) 110, 148
BstSCI CCNGG 2 cut(s) 108, 146
BstUI CGCG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 54
BstYI RGATCY 1 cut(s) 54
BsuRI GGCC 3 cut(s) 5, 129, 294
BtsCI GGATG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 71, 156, 485
Cac8I GCNNGC 1 cut(s) 517
CaiI CAGNNNCTG 1 cut(s) 329
CfoI GCGC 2 cut(s) 31, 246
CviAII CATG 1 cut(s) 235
CviJI RGCY 6 cut(s) 5, 108, 129, 185, 239, 294
CviKI_1 RGCY 6 cut(s) 5, 108, 129, 185, 239, 294
DpnI GATC 3 cut(s) 56, 350, 405
DpnII GATC 3 cut(s) 54, 348, 403
EaeI YGGCCR 2 cut(s) 3, 292
EciI GGCGGA 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 108, 146
EcoT14I CCWWGG 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 1 cut(s) 238
FaqI GGGAC 1 cut(s) 424
FatI CATG 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 6, 186, 336
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 3 cut(s) 6, 186, 336
FspBI CTAG 1 cut(s) 516
FspI TGCGCA 1 cut(s) 245
GlaI GCGC 2 cut(s) 30, 245
GluI GCNGC 3 cut(s) 6, 186, 336
HaeIII GGCC 3 cut(s) 5, 129, 294
HapII CCGG 1 cut(s) 23
HhaI GCGC 2 cut(s) 31, 246
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 2 cut(s) 29, 244
HinP1I GCGC 2 cut(s) 29, 244
HinfI GANTC 1 cut(s) 204
HpaII CCGG 1 cut(s) 23
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 501
Hpy188I TCNGA 1 cut(s) 203
Hpy188III TCNNGA 4 cut(s) 58, 250, 352, 450
Hpy8I GTNNAC 1 cut(s) 501
HpyAV CCTTC 2 cut(s) 260, 292
HpyCH4III ACNGT 3 cut(s) 75, 331, 489
HpyCH4V TGCA 3 cut(s) 80, 467, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 332
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 2 cut(s) 29, 244
Kzo9I GATC 3 cut(s) 54, 348, 403
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 2 cut(s) 89, 255
MaeI CTAG 1 cut(s) 516
MaeIII GTNAC 2 cut(s) 97, 319
MalI GATC 3 cut(s) 56, 350, 405
MboI GATC 3 cut(s) 54, 348, 403
MboII GAAGA 3 cut(s) 386, 397, 438
MflI RGATCY 1 cut(s) 54
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 2 cut(s) 33, 165
MluNI TGGCCA 1 cut(s) 294
MnlI CCTC 6 cut(s) 30, 45, 132, 140, 248, 426
Mox20I TGGCCA 1 cut(s) 294
MscI TGGCCA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 294
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 2 cut(s) 110, 148
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 2 cut(s) 110, 148
MvnI CGCG 1 cut(s) 31
MwoI GCNNNNNNNGC 2 cut(s) 236, 332
NdeII GATC 3 cut(s) 54, 348, 403
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 97
NsbI TGCGCA 1 cut(s) 245
PctI GAATGC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 204
PkrI GCNGC 3 cut(s) 7, 187, 337
PsiI TTATAA 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 108, 146
PspGI CCWGG 2 cut(s) 108, 146
PspN4I GGNNCC 1 cut(s) 56
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 1 cut(s) 54
SatI GCNGC 3 cut(s) 6, 186, 336
Sau3AI GATC 3 cut(s) 54, 348, 403
ScrFI CCNGG 2 cut(s) 110, 148
SetI ASST 5 cut(s) 126, 149, 199, 303, 437
SfaNI GCATC 2 cut(s) 89, 255
Sse9I AATT 2 cut(s) 33, 165
SsiI CCGC 4 cut(s) 6, 17, 336, 395
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 2 cut(s) 108, 146
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 3 cut(s) 75, 331, 489
TaqI TCGA 1 cut(s) 308
TasI AATT 2 cut(s) 33, 165
TauI GCSGC 2 cut(s) 8, 338
TfiI GAWTC 1 cut(s) 204
TscAI CASTG 3 cut(s) 78, 163, 492
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 3 cut(s) 76, 177, 279
TspRI CASTG 3 cut(s) 78, 163, 492
XspI CTAG 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.