Rh1DG332600

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017713
Physical Location & Seq
Forward (+)
55794397 .. 55795705
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG332600.1

Sequence Viewer

Length: 528 bp
ATGGCCTTTATGCTTTCGCCTGGGGTCTCGCGAATCGTTACTATTGTATTGAGGGTCCTGACTGCCATTGTTCTGTTTGTATCACTCGTGATGCTCGTAATCAATAATCAGGATTATCAAGACCCTAATGACAACGGAAACACTAAAACCGCTCGTTTTTATGATCAAGTTGGATTCCAATATATGGCTGCCACAACAGTTCTCGGAATTGGGTTTTCAATCTATGGAACCGTAATTGTAGCTTTGCGCATCAAGAGAGGAAATGATGAAGGGAACCTGTTGATTGATTTCTATGGCCAGAAGGTTTTGTCGAATTTATTAGTTACAGGAGCTGTTGCAGGATTTCTTACGGTCCAAGCTGTGGATAAAGCTCTCTCTGATCACTTTGGCACGTTCGCGGAGCTGGCCACTAACGGCTATTATTGGGGTTTGTATAAGACATGTTCTGGACTTGTCCTCCTTGGCTTCTTCTTGTCTGTAGCACTGTCGATCCTGTCTTCCCATACCCTTGCTAGAAGGAATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

175

Amino Acids

19.12

Weight (kDa)

8.71

Isoelectric Point (pI)

17.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 11 - 124 4.1e-11 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017713)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g22230
rosa_chinensis RchiOBHm_Chr1g0366621
rosa_laevigata RLG00000027332
rosa_multiflora Rmu_sc0003180.1_g000008
rosa_roxburghii Rroxscaffold_4G00289810
rosa_rugosa Rorug01G0330900 Rorug01G0331000
rosa_samantha Rh1AG339300 Rh1BG301000 Rh1CG315800 Rh1DG332600
rosa_wichuraiana Rw1G030050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 248
AccB7I CCANNNNNTGG 2 cut(s) 184, 361
AccBSI CCGCTC 1 cut(s) 152
AccII CGCG 2 cut(s) 31, 398
AciI CCGC 2 cut(s) 150, 398
AclWI GGATC 1 cut(s) 484
AcoI YGGCCR 2 cut(s) 295, 405
AcsI RAATTY 1 cut(s) 313
AfiI CCNNNNNNNGG 2 cut(s) 184, 361
AflIII ACRYGT 1 cut(s) 440
AgsI TTSAA 1 cut(s) 219
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 5 cut(s) 242, 332, 359, 371, 403
AluI AGCT 5 cut(s) 242, 332, 359, 371, 403
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 1 cut(s) 484
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 3 cut(s) 3, 295, 405
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 313
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 2 cut(s) 55, 352
AvaII GGWCC 2 cut(s) 55, 352
BalI TGGCCA 2 cut(s) 297, 407
BauI CACGAG 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 21
BclI TGATCA 2 cut(s) 163, 379
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 1 cut(s) 513
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 21
Bme18I GGWCC 2 cut(s) 55, 352
BmgT120I GGNCC 2 cut(s) 55, 352
BmiI GGNNCC 3 cut(s) 56, 229, 275
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 2 cut(s) 81, 258
BpiI GAAGAC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 2 cut(s) 20, 460
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 361
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 2 cut(s) 20, 460
BseLI CCNNNNNNNGG 2 cut(s) 184, 361
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 2 cut(s) 31, 398
BshFI GGCC 3 cut(s) 5, 297, 407
BslI CCNNNNNNNGG 2 cut(s) 184, 361
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 3 cut(s) 5, 297, 407
Bso31I GGTCTC 1 cut(s) 31
Bsp143I GATC 3 cut(s) 163, 379, 489
Bsp68I TCGCGA 1 cut(s) 31
BspACI CCGC 2 cut(s) 150, 398
BspANI GGCC 3 cut(s) 5, 297, 407
BspFNI CGCG 2 cut(s) 31, 398
BspLI GGNNCC 3 cut(s) 56, 229, 275
BspPI GGATC 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 31
BsrBI CCGCTC 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 20, 460
BssMI GATC 3 cut(s) 163, 379, 489
BssSI CACGAG 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 460
Bst2BI CACGAG 1 cut(s) 86
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 4 cut(s) 199, 232, 352, 486
BstC8I GCNNGC 1 cut(s) 405
BstFNI CGCG 2 cut(s) 31, 398
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 3 cut(s) 166, 382, 492
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 3 cut(s) 163, 379, 489
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 1 cut(s) 21
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 477
BstUI CGCG 2 cut(s) 31, 398
BstV1I GCAGC 1 cut(s) 175
BstV2I GAAGAC 1 cut(s) 489
BsuRI GGCC 3 cut(s) 5, 297, 407
BtsIMutI CAGTG 1 cut(s) 482
BtuMI TCGCGA 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 405
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 2 cut(s) 55, 352
CviAII CATG 1 cut(s) 441
DpnI GATC 3 cut(s) 165, 381, 491
DpnII GATC 3 cut(s) 163, 379, 489
EaeI YGGCCR 2 cut(s) 295, 405
Eco130I CCWWGG 1 cut(s) 460
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 2 cut(s) 55, 352
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 460
FaeI CATG 1 cut(s) 444
FaiI YATR 9 cut(s) 11, 162, 183, 185, 225, 294, 435, 442, 504
FatI CATG 1 cut(s) 440
FbaI TGATCA 2 cut(s) 163, 379
Fnu4HI GCNGC 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 1 cut(s) 513
FspI TGCGCA 1 cut(s) 248
GlaI GCGC 1 cut(s) 248
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 3 cut(s) 5, 297, 407
HhaI GCGC 1 cut(s) 249
Hin1II CATG 1 cut(s) 444
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HinfI GANTC 2 cut(s) 33, 174
Hpy188I TCNGA 2 cut(s) 206, 379
Hpy188III TCNNGA 7 cut(s) 30, 58, 88, 110, 119, 253, 447
HpyAV CCTTC 3 cut(s) 263, 295, 510
HpyCH4III ACNGT 4 cut(s) 199, 232, 352, 486
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 1 cut(s) 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpySE526I ACGT 1 cut(s) 392
Hsp92II CATG 1 cut(s) 444
HspAI GCGC 1 cut(s) 247
Ksp22I TGATCA 2 cut(s) 163, 379
Kzo9I GATC 3 cut(s) 163, 379, 489
LmnI GCTCC 2 cut(s) 329, 400
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 2 cut(s) 81, 258
MaeI CTAG 1 cut(s) 513
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 37, 322
MalI GATC 3 cut(s) 165, 381, 491
MbiI CCGCTC 1 cut(s) 152
MboI GATC 3 cut(s) 163, 379, 489
MboII GAAGA 2 cut(s) 460, 489
MlsI TGGCCA 2 cut(s) 297, 407
MluCI AATT 4 cut(s) 207, 234, 313, 520
MluNI TGGCCA 2 cut(s) 297, 407
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 3 cut(s) 45, 251, 467
Mox20I TGGCCA 2 cut(s) 297, 407
MscI TGGCCA 2 cut(s) 297, 407
Msp20I TGGCCA 2 cut(s) 297, 407
MspR9I CCNGG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 21
MvnI CGCG 2 cut(s) 31, 398
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeII GATC 3 cut(s) 163, 379, 489
NlaIII CATG 1 cut(s) 444
NlaIV GGNNCC 3 cut(s) 56, 229, 275
NruI TCGCGA 1 cut(s) 31
NsbI TGCGCA 1 cut(s) 248
NspI RCATGY 1 cut(s) 444
PciI ACATGT 1 cut(s) 440
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 2 cut(s) 33, 174
PflMI CCANNNNNTGG 2 cut(s) 184, 361
PkrI GCNGC 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 55
PscI ACATGT 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 19
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 3 cut(s) 56, 229, 275
PspPI GGNCC 2 cut(s) 55, 352
PspPPI RGGWCCY 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 332
RruI TCGCGA 1 cut(s) 31
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 3 cut(s) 163, 379, 489
Sau96I GGNCC 2 cut(s) 55, 352
ScrFI CCNGG 1 cut(s) 21
SetI ASST 8 cut(s) 244, 279, 306, 334, 361, 373, 395, 405
SfaNI GCATC 2 cut(s) 81, 258
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 2 cut(s) 55, 352
Sse9I AATT 4 cut(s) 207, 234, 313, 520
SsiI CCGC 2 cut(s) 150, 398
SspMI CTAG 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 19
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 4 cut(s) 199, 232, 352, 486
TaiI ACGT 1 cut(s) 395
TaqI TCGA 2 cut(s) 311, 488
TasI AATT 4 cut(s) 207, 234, 313, 520
TfiI GAWTC 2 cut(s) 33, 174
TscAI CASTG 1 cut(s) 489
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 282
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 1 cut(s) 489
Van91I CCANNNNNTGG 2 cut(s) 184, 361
VpaK11BI GGWCC 2 cut(s) 55, 352
XapI RAATTY 1 cut(s) 313
XceI RCATGY 1 cut(s) 444
XspI CTAG 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.