Rorug01G0331000

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017713
Physical Location & Seq
Reverse (-)
44952497 .. 44962240
9744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0331000.1

Sequence Viewer

Length: 4950 bp
ATGGCCTCGAAGGAAAAAGGAAAGGCGATCGCCACAAGCGGATCGTCCGCCGGCGGCAAGCGGAAGCGGAGAAACCCCGGCGTTCTCCAGTTCTTCGAGCACTCCGCCGCCGAAGCTGACGGTTCGGACGACGACAGCGACTTGGACGATTTTATGGAAGAAGAACTTGAAACAGAACCTATAGTCAAGAGTGAACCGGGGAAAGCCCATAACCTTCCATTTGTCCCCAAAGAGGAGGATATTGATGGGGACGAATTTGAAAGAATGATGGAAGAACGATACAGGACTGGTTCTACATATGTTACCTATGCTGAAGATAATTATGAAAACAAAAGATCGGTTGATGGAATTGTGCTTGAGCCTTCTGCTAAAGATCCTGTTATCTGGAAAGTAAAGTGTGCGGTTGGACGCGAGAGACACTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTACGATCAATGGGTACCAAGCTGCAGATAATATCTGCATTTGCTGTTGACCACATCAAGGGTTTTGTTTTCATTGAAGCTGATAAGCTATGTGACGTTCAGGAGGCATGTAAAGGACTTTGTAATATATTTTTATCCCGAGTAACGCCAGTTCCCAAAAATGAAGCTCCTCATTTACTGGCTCCTCGGACTAGAAACAGTGAAATTACTGTGGGTACATGGGCCCGTGTGAAGAGTGGGAACTACAAGGGTGACTTGGCACAGGTTGTGGCTGTGAACGAAAAGAAAAAAGCAACAGTAAAGCTGATACCAAGAATTGATCTACAAGCAATGGCTGCAAAATTTGGTGGGGGAGTTACTGGAAAGAAACTGCCTACTCCAGCACCGAGATTGATCAGCACAAGCGAACTTGAGGAGTTCCGCCCTCTCATTCAGGACAGGAAGGATAAGGATACCGGATTGCGTTTTCTCTGTCTTGATGGTTTGCTCCTTAAGGATGGATATTTATACAAAAAAATGCCATTAGATTCATTAAATTGCTGGGGTGTCATGCCATCAGAAGAGGAGATACTGAAATTCAAGCCTTCTGAGAACAATGAATCTGCTGATCTGGAGTGGCTTTCACAGCTTTATGGTGACAATAAGAAAAGGAAGAGCATCGGCAGTGATAAAGGGGATGGCAAAGGAGAAGGTTCAGCAGGGTGTGGTGGGAAAGGAGAAGGTTCATCAAGGGGTAACGGGAAGGGAGAAGGTCCTTCAGGTGGGAAAGAAGGTTCTTCAGGGTTTACTGGGAAAGGAGAAGGTTCTTCAGGGTGTGGTGGGAATCTCTATGAGCTGTATGATCTTGTTTGTTTCGGCAAGAAAGATTTTGGTCTTGTGCTAGGCATTGAGAAAGATGATACTTACAAGATTCTGAAAGAGGGTGTGGAAGGATCTACTGTTGTGACAGTTCAGCAGAATGAGATAAAGAATGTACTTTCTGATGTAAAATTTACAGCTTTAGATCAGCGCCTGAAGCCCATTCGCGTGAATGATACAGTTAAAGTTTTGGAAGGACCATTGAAGGATAGGCAAGGAATAGTGAAGCAAGTCTACAGAGGCATAATATTCATGTTTGATGAGAATGAGACAGAAAATGGTGGTTATTTCTGCTCAAAATCTCAAATGTGTGAGAAAATCAAGCTTTCCATTGACGTTATCCCAGAAAAGGATGGGGATTCGGGTGCTTTCGGTTTTGAAGATTTCACATCATCACCTAAATCCCCTCTATCGCCAAAGAAGCCTTGGCTAGGAAGGGAGAATAACTTCAATCAGGGCGATACTGACGGAATGTTCTCAATCGGTCAAACTTTAAGAATTCGTGTTGGTCCATTAAAGGGATACCTCTGTCGTGTCTTGGCCATACGCCGTTCTGATATTACAGTTAAGCTTGATTCTCAGCAAAGGGTTCTTACAGTTAAAGCTGAGCATCTTACAGAGGTTCGTGTAAAGAGCGCTGCCATGCTTAGTGAGGATCCGGAGTCCAGCTCTCTTAAACCATTTGATTTACTTGGAACTGAAGGAGGCTCTAAAGATTGGACAGATGGAGCTGGGACATCAGCCGGGGGTGATGGGTGGAATGCTGGAGGGTCATCTGGTGAAAGGAATGCTTGGCCTAGTTTCTCTGCCTCGGGAAATTCGCTTCAGCCTGAGTCCAGCTCTGCGAATCCTTTTGAATCTGATGGAAATGGTGCTAAAAAAGAGGATAGTTCTTGGGAGAGGAACGTGGCTCCAAATAACAACTCATCCTGGGGTGTTGCAGCAGCAGAAAACAAGGATCAAGTTACTGGCTGGGGAAAAAGCAACTCTTGGGGCGCTAAAGTTGGAGGTGACAGCAGTCTGTCAGATACTTTGCAAAAAGCATCAGAACCTGCCAGTTCATCCTGGGGTGTTGCAGCAGCAGTAGATAACAAGGATCAAGGTACTGGCTGGGGAAAAAGTGACTCTTGGGGCGCTAAAGTTGGAGGTGACAGCACTTCGTCGGATACTTGGCAAAAAGCATCAGCGCCTGCTAGTTCATCCTGGGGTGTTGCGGCAGCAATAGATAAGAAGGATCAAGGTACTGGCTGGGGTGCTAAGACTGGATGTGACGGCACTGCATCAGATAGTTGGAAAAAAGCATCAGAACCTGCTAGTAATTCATCCTGGGGTGTTGCGGCAGCAATAGATAAGAAGGATCAAGGTACTGGCTGGGGTGCTAAGACTGGATGTGACGGCACTGCGTCAGATAGTTGGAAAAAAGCATCAGAACCTGCTAGTAATTCGTCCTGGGGTGTTGCAGCAGCAGCAGATAACAAGGATCAAGGTTCCGTCTGGGGCGGTAATGGTAGTCAAAAACCAAGTGTTGCAGATGATAGTGGTGACAAAGGTTCTGCTTGGAATAAGCCAGCTGAAGGTTCTTGGAGTAAACAAACTGGAGGATCCTCATGGGGCAAGCAAGCCGATTGGAGCACTAAAGAGGGATCAAGTACTGGTGATGCAGGGTGGAAAAGTTCAACGCCTTCAGTAGAGAATCAGACTGGGAGCTGGGGTAATGCAGGTGGCTGGGCAAAAGCTCAAGGCATATCGGGTAATGAGAATCCTAAAGACAGTTGGAAGAAACCAAGTGGTATAGATGACAATAAAAATGCTTCGTGGATCAAGGAAGATGGAGGTTCTACCTGGAATAAACAAGATGGAGGTTCTACTTGGAATAAGCCTGCTGGAGGTTCTTGGAGTAAAGGTGGGGATCAAGGGTCTGGTGGTGGCTATGGTGGCGATGGTGGTGGTGGTAGAGGGGGTGGAGGTGGGAACTGTTTTAAGTGTGGTGAGTCTGGACACATGTCTAGGGAGTGTCCTCAGGGTGGCGGTGGTGGTAGAGGGAGTGGAGGTGGGAACTGTTTCAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGTGGTGGTGGTGGTAGAGGGGGTGGAGGTGGGAATTGTTTCAAGTGCGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGTGGTGGTGGTGGTGGTAGAGGGGGGAACTGTTACAAGTGTGGTGAATCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGAGGTGGTGGTAGAGGGGGTGGAGGTGGTAACTGTTTCAAGTGCGGTGAATCTGGACATATGTCAAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGCAGGAGGTGGTAACTGTTTCAAGTGCGGTGAGTCTGGACATATGTCAAGAGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGCTGGAGGTGGGAACTGTTACAAGTGTGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGGCTGGAGGTGGGAACTGTTACAAGTGTGGTGAGTCTGGACACATGTCTAGGGAGTGCCCTCAGGGCGGCGGTGGTGGTAGAGGGAGTGGGGGTGGGAACTGTTACAAGTGTGGTGAGTCTGGACACATGTCTAGGGAGTGTCCTCAGGGCGGCGGTGGTAGAGGGGCTGGAAGTGGGAACTGTTTCAAGTGTGGTGAGTCTGGACACATGGCTAGGGACTGCTCTCAGGGAGGTGGTAGCTATGGCAACTTCGGTGGTGGAGCAAAGTCCACTGATTCATGGAGCAAGCGTGCTTGGGGTACTGGAGATGGTGGAAGTGGCGGTGATGGAGCAAAACCAGCAGCAAGCTCTTGGGGTACCACAGATGGTGGAGCAAAGGCCACTGATTCATGGAGCAAGCGTGCTTGGGGTACTGGAGAAGGTGGAAGTGGCAGCGGTGGACCAAAACCAGCAGCAAGCTCTTGGGGTACAGGAAGCTCTTGGGGTACAAATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAACCAGCAGCAAGCTCTTGGGGTAAGGGGGATGGTGGAAGTGGCGGACATGGTGATGAAGCAAAACCAGCAGCAAGCTCTTGGGGTAAGGGGGATGGTGGAAGTGGCGGACATGGTGATGAAGCAAAACCAGCAGCAAGCACTTGGGGTACAGGAAGCTCTTGGGGTAAGGCAGATGGTGGAAGTGGCAGAGCTAGTGGTGAAGCAAAACCAGCAGCAAGCTCTTGGGGTACAAATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAACCAGCAGCAAGCTCTTGGGGTAAGGGGGATGGTGGAAGTGGCAGAGCTAGTGGTGAAGCAAAACCAGCAGCAAGCACTTGGGGTACAGGAAGCTCCTGGGGTAAGGGAGATGGTGGAAGTGGCGGAGCTGGTGGTGAAGCAAAGCCTGCTGATCCATGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGGGAGATGGCGGAAGTGGCGGAGCTAGTGGTGAAGCAAAGCCTGCTGATCCTTGGAGCAAGCCATCAGCAAGTGCTTGGGGTAAGAAAGATGGTGGAAGTGGCAGCGGAGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

1649

Amino Acids

168.05

Weight (kDa)

6.98

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 128 - 211 2e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 222 - 344 3.4e-25 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 425 - 475 3.8e-10 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 484 - 538 4e-22 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 598 - 642 2.1e-06 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 1095 - 1111 2.1e-06 Zinc knuckle
zf-CCHC PF00098 1123 - 1139 2.1e-06 Zinc knuckle
zf-CCHC PF00098 1151 - 1167 2.1e-06 Zinc knuckle
zf-CCHC PF00098 1177 - 1193 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1205 - 1221 2.1e-06 Zinc knuckle
zf-CCHC PF00098 1233 - 1249 2.1e-06 Zinc knuckle
zf-CCHC PF00098 1261 - 1277 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1289 - 1305 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1317 - 1333 1.1e-06 Zinc knuckle
zf-CCHC PF00098 1344 - 1360 2.9e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017713)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g22230
rosa_chinensis RchiOBHm_Chr1g0366621
rosa_laevigata RLG00000027332
rosa_multiflora Rmu_sc0003180.1_g000008
rosa_roxburghii Rroxscaffold_4G00289810
rosa_rugosa Rorug01G0330900 Rorug01G0331000
rosa_samantha Rh1AG339300 Rh1BG301000 Rh1CG315800 Rh1DG332600
rosa_wichuraiana Rw1G030050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3020
Acc36I ACCTGC 4 cut(s) 2371, 2629, 2752, 3020
Acc65I GGTACC 2 cut(s) 467, 4208
AccB1I GGYRCC 2 cut(s) 467, 4208
AccI GTMKAC 1 cut(s) 1545
AccII CGCG 2 cut(s) 411, 1479
AccIII TCCGGA 1 cut(s) 1969
AcoI YGGCCR 1 cut(s) 1851
AcsI RAATTY 6 cut(s) 254, 794, 1028, 1442, 1809, 2128
AcuI CTGAAG 9 cut(s) 333, 1194, 1215, 1245, 1487, 2031, 2120, 2904, 2979
AfeI AGCGCT 1 cut(s) 1948
AflII CTTAAG 1 cut(s) 944
AflIII ACRYGT 7 cut(s) 3312, 3396, 3480, 3558, 3810, 3894, 3978
AjnI CCWGG 7 cut(s) 2240, 2375, 2513, 2636, 2759, 3152, 4739
AloI GAACNNNNNNTCC 2 cut(s) 1210, 1242
Alw21I GWGCWC 2 cut(s) 102, 2945
Alw26I GTCTC 2 cut(s) 409, 1574
AlwNI CAGNNNCTG 5 cut(s) 1465, 2363, 2501, 2621, 2744
Ama87I CYCGRG 2 cut(s) 591, 2122
Aor13HI TCCGGA 1 cut(s) 1969
Aor51HI AGCGCT 1 cut(s) 1948
AoxI GGCC 5 cut(s) 3, 675, 1851, 2105, 4230
ApaI GGGCCC 1 cut(s) 679
ApoI RAATTY 6 cut(s) 254, 794, 1028, 1442, 1809, 2128
AsiSI GCGATCGC 1 cut(s) 30
Asp700I GAANNNNTTC 4 cut(s) 1757, 3371, 3455, 4034
Asp718I GGTACC 2 cut(s) 467, 4208
AspLEI GCGC 5 cut(s) 1464, 1949, 2309, 2447, 2500
AspS9I GGNCC 6 cut(s) 675, 676, 1205, 1508, 1820, 4292
AsuC2I CCSGG 3 cut(s) 78, 198, 2056
AvaI CYCGRG 2 cut(s) 591, 2122
AvaII GGWCC 4 cut(s) 1205, 1508, 1820, 4292
AxyI CCTNAGG 9 cut(s) 3330, 3414, 3498, 3576, 3660, 3744, 3828, 3912, 3996
BaeGI GKGCMC 8 cut(s) 679, 3413, 3497, 3575, 3659, 3743, 3827, 3911
BaeI ACNNNNGTAYC 2 cut(s) 1825, 1858
BalI TGGCCA 1 cut(s) 1853
BamHI GGATCC 2 cut(s) 1966, 2912
BanI GGYRCC 2 cut(s) 467, 4208
BanII GRGCYC 1 cut(s) 679
BarI GAAGNNNNNNTAC 4 cut(s) 1005, 1037, 1529, 1561
Bbv12I GWGCWC 2 cut(s) 102, 2945
BceAI ACGGC 3 cut(s) 1845, 2599, 2722
BciT130I CCWGG 7 cut(s) 2242, 2377, 2515, 2638, 2761, 3154, 4741
BciVI GTATCC 3 cut(s) 898, 1826, 2470
BclI TGATCA 1 cut(s) 846
BcnI CCSGG 3 cut(s) 78, 198, 2056
BcoDI GTCTC 2 cut(s) 409, 1574
BfmI CTRYAG 3 cut(s) 180, 476, 1546
BfoI RGCGCY 5 cut(s) 1465, 1950, 2310, 2448, 2501
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 4 cut(s) 2371, 2629, 2752, 3020
BfuI GTATCC 3 cut(s) 898, 1826, 2470
BglI GCCNNNNNGGC 5 cut(s) 3579, 3663, 3747, 3831, 3915
BlpI GCTNAGC 1 cut(s) 1917
BmcAI AGTACT 1 cut(s) 2962
Bme18I GGWCC 4 cut(s) 1205, 1508, 1820, 4292
BmeT110I CYCGRG 2 cut(s) 591, 2122
BmgT120I GGNCC 6 cut(s) 675, 676, 1205, 1508, 1820, 4292
BmiI GGNNCC 8 cut(s) 469, 636, 677, 1968, 2223, 2800, 2914, 4210
BmrI ACTGGG 2 cut(s) 1251, 3021
BmsI GCATC 9 cut(s) 426, 1119, 1930, 2363, 2501, 2600, 2621, 2744, 2959
BmuI ACTGGG 2 cut(s) 1251, 3021
BplI GAGNNNNNCTC 6 cut(s) 404, 436, 1964, 1996, 2135, 2167
Bpu1102I GCTNAGC 1 cut(s) 1917
BpuEI CTTGAG 3 cut(s) 377, 884, 3033
BpuMI CCSGG 3 cut(s) 78, 198, 2056
BsaWI WCCGGW 2 cut(s) 908, 1969
Bse118I RCCGGY 1 cut(s) 50
Bse21I CCTNAGG 9 cut(s) 3330, 3414, 3498, 3576, 3660, 3744, 3828, 3912, 3996
Bse3DI GCAATG 1 cut(s) 789
BseAI TCCGGA 1 cut(s) 1969
BseBI CCWGG 7 cut(s) 2242, 2377, 2515, 2638, 2761, 3154, 4741
BseMI GCAATG 1 cut(s) 789
BseRI GAGGAG 5 cut(s) 248, 612, 627, 881, 1031
BseSI GKGCMC 8 cut(s) 679, 3413, 3497, 3575, 3659, 3743, 3827, 3911
BseYI CCCAGC 8 cut(s) 993, 2042, 2283, 2421, 2559, 2682, 3018, 3036
Bsh1236I CGCG 2 cut(s) 411, 1479
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 5 cut(s) 5, 677, 1853, 2107, 4232
BshNI GGYRCC 2 cut(s) 467, 4208
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 102, 2945
BsiHKCI CYCGRG 2 cut(s) 591, 2122
BsiSI CCGG 6 cut(s) 51, 78, 197, 909, 1970, 2055
BslFI GGGAC 4 cut(s) 209, 263, 2059, 4082
BsmAI GTCTC 2 cut(s) 409, 1574
BsmFI GGGAC 4 cut(s) 209, 263, 2059, 4082
BsmI GAATGC 2 cut(s) 2077, 2104
BsnI GGCC 5 cut(s) 5, 677, 1853, 2107, 4232
BsoBI CYCGRG 2 cut(s) 591, 2122
Bsp120I GGGCCC 1 cut(s) 675
Bsp13I TCCGGA 1 cut(s) 1969
Bsp1720I GCTNAGC 1 cut(s) 1917
Bsp19I CCATGG 1 cut(s) 4799
BspANI GGCC 5 cut(s) 5, 677, 1853, 2107, 4232
BspEI TCCGGA 1 cut(s) 1969
BspFNI CGCG 2 cut(s) 411, 1479
BspLI GGNNCC 8 cut(s) 469, 636, 677, 1968, 2223, 2800, 2914, 4210
BspMAI CTGCAG 1 cut(s) 480
BspMI ACCTGC 4 cut(s) 2371, 2629, 2752, 3020
BspQI GCTCTTC 1 cut(s) 1100
BspT107I GGYRCC 2 cut(s) 467, 4208
BspTI CTTAAG 1 cut(s) 944
BsrDI GCAATG 1 cut(s) 789
BsrFI RCCGGY 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 50
BssT1I CCWWGG 3 cut(s) 1736, 4799, 4883
Bst2UI CCWGG 7 cut(s) 2242, 2377, 2515, 2638, 2761, 3154, 4741
Bst6I CTCTTC 3 cut(s) 680, 1008, 1100
BstAFI CTTAAG 1 cut(s) 944
BstAPI GCANNNNNTGC 3 cut(s) 788, 4790, 4874
BstDSI CCRYGG 1 cut(s) 4799
BstENI CCTNNNNNAGG 2 cut(s) 1740, 3195
BstFNI CGCG 2 cut(s) 411, 1479
BstH2I RGCGCY 5 cut(s) 1465, 1950, 2310, 2448, 2501
BstHHI GCGC 5 cut(s) 1464, 1949, 2309, 2447, 2500
BstMAI GTCTC 2 cut(s) 409, 1574
BstMCI CGRYCG 1 cut(s) 30
BstNI CCWGG 7 cut(s) 2242, 2377, 2515, 2638, 2761, 3154, 4741
BstNSI RCATGY 8 cut(s) 564, 3316, 3400, 3484, 3562, 3814, 3898, 3982
BstSFI CTRYAG 3 cut(s) 180, 476, 1546
BstSLI GKGCMC 8 cut(s) 679, 3413, 3497, 3575, 3659, 3743, 3827, 3911
BstUI CGCG 2 cut(s) 411, 1479
BstX2I RGATCY 4 cut(s) 373, 1385, 1966, 2912
BstYI RGATCY 4 cut(s) 373, 1385, 1966, 2912
Bsu36I CCTNAGG 9 cut(s) 3330, 3414, 3498, 3576, 3660, 3744, 3828, 3912, 3996
BsuI GTATCC 3 cut(s) 898, 1826, 2470
BsuRI GGCC 5 cut(s) 5, 677, 1853, 2107, 4232
BtgI CCRYGG 1 cut(s) 4799
BtgZI GCGATG 1 cut(s) 3264
BtsI GCAGTG 3 cut(s) 1123, 2586, 2709
BtsIMutI CAGTG 6 cut(s) 658, 1123, 2586, 2709, 4122, 4233
BveI ACCTGC 4 cut(s) 2371, 2629, 2752, 3020
CaiI CAGNNNCTG 5 cut(s) 1465, 2363, 2501, 2621, 2744
CfoI GCGC 5 cut(s) 1464, 1949, 2309, 2447, 2500
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 6 cut(s) 675, 676, 1205, 1508, 1820, 4292
CseI GACGC 2 cut(s) 417, 2703
CspCI CAANNNNNGTGG 2 cut(s) 4087, 4122
EaeI YGGCCR 1 cut(s) 1851
Eam1104I CTCTTC 3 cut(s) 680, 1008, 1100
EarI CTCTTC 3 cut(s) 680, 1008, 1100
Eco130I CCWWGG 3 cut(s) 1736, 4799, 4883
Eco24I GRGCYC 1 cut(s) 679
Eco47I GGWCC 4 cut(s) 1205, 1508, 1820, 4292
Eco47III AGCGCT 1 cut(s) 1948
Eco57I CTGAAG 9 cut(s) 333, 1194, 1215, 1245, 1487, 2031, 2120, 2904, 2979
Eco81I CCTNAGG 9 cut(s) 3330, 3414, 3498, 3576, 3660, 3744, 3828, 3912, 3996
Eco88I CYCGRG 2 cut(s) 591, 2122
EcoNI CCTNNNNNAGG 2 cut(s) 1740, 3195
EcoO109I RGGNCCY 1 cut(s) 1205
EcoRI GAATTC 1 cut(s) 1809
EcoRII CCWGG 7 cut(s) 2240, 2375, 2513, 2636, 2759, 3152, 4739
EcoT14I CCWWGG 3 cut(s) 1736, 4799, 4883
EcoT38I GRGCYC 1 cut(s) 679
ErhI CCWWGG 3 cut(s) 1736, 4799, 4883
FaqI GGGAC 4 cut(s) 209, 263, 2059, 4082
FauNDI CATATG 3 cut(s) 298, 3642, 3726
FbaI TGATCA 1 cut(s) 846
FblI GTMKAC 1 cut(s) 1545
FriOI GRGCYC 1 cut(s) 679
GlaI GCGC 5 cut(s) 1463, 1948, 2308, 2446, 2499
GsaI CCCAGC 8 cut(s) 997, 2046, 2287, 2425, 2563, 2686, 3022, 3040
HaeII RGCGCY 5 cut(s) 1465, 1950, 2310, 2448, 2501
HaeIII GGCC 5 cut(s) 5, 677, 1853, 2107, 4232
HapII CCGG 6 cut(s) 51, 78, 197, 909, 1970, 2055
HgaI GACGC 2 cut(s) 417, 2703
HhaI GCGC 5 cut(s) 1464, 1949, 2309, 2447, 2500
Hin6I GCGC 5 cut(s) 1462, 1947, 2307, 2445, 2498
HinP1I GCGC 5 cut(s) 1462, 1947, 2307, 2445, 2498
HincII GTYRAC 2 cut(s) 451, 502
HindII GTYRAC 2 cut(s) 451, 502
HindIII AAGCTT 2 cut(s) 1634, 1880
HpaII CCGG 6 cut(s) 51, 78, 197, 909, 1970, 2055
Hpy166II GTNNAC 9 cut(s) 194, 451, 502, 730, 1239, 1546, 2900, 4122, 4292
Hpy8I GTNNAC 9 cut(s) 194, 451, 502, 730, 1239, 1546, 2900, 4122, 4292
Hpy99I CGWCG 2 cut(s) 134, 2476
HpyCH4IV ACGT 3 cut(s) 549, 1647, 2217
HpySE526I ACGT 3 cut(s) 549, 1647, 2217
HspAI GCGC 5 cut(s) 1462, 1947, 2307, 2445, 2498
Kpn2I TCCGGA 1 cut(s) 1969
KpnI GGTACC 2 cut(s) 471, 4212
KroI GCCGGC 1 cut(s) 50
KroNI GCCGGC 1 cut(s) 52
Ksp22I TGATCA 1 cut(s) 846
LguI GCTCTTC 1 cut(s) 1100
LweI GCATC 9 cut(s) 426, 1119, 1930, 2363, 2501, 2600, 2621, 2744, 2959
MaeII ACGT 3 cut(s) 549, 1647, 2217
MflI RGATCY 4 cut(s) 373, 1385, 1966, 2912
MlsI TGGCCA 1 cut(s) 1853
MluNI TGGCCA 1 cut(s) 1853
MmeI TCCRAC 7 cut(s) 385, 2296, 2434, 2454, 2582, 2705, 3065
Mox20I TGGCCA 1 cut(s) 1853
MreI CGCCGGCG 1 cut(s) 50
MroI TCCGGA 1 cut(s) 1969
MroNI GCCGGC 1 cut(s) 50
MroXI GAANNNNTTC 4 cut(s) 1757, 3371, 3455, 4034
MscI TGGCCA 1 cut(s) 1853
MseI TTAA 9 cut(s) 945, 986, 1494, 1805, 1826, 1878, 1911, 1986, 3291
MslI CAYNNNNRTG 3 cut(s) 1478, 4428, 4491
Msp20I TGGCCA 1 cut(s) 1853
MspA1I CMGCKG 3 cut(s) 2882, 4287, 4938
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 6 cut(s) 51, 78, 197, 909, 1970, 2055
Mva1269I GAATGC 2 cut(s) 2077, 2104
MvaI CCWGG 7 cut(s) 2242, 2377, 2515, 2638, 2761, 3154, 4741
MvnI CGCG 2 cut(s) 411, 1479
NaeI GCCGGC 1 cut(s) 52
NciI CCSGG 3 cut(s) 78, 198, 2056
NcoI CCATGG 1 cut(s) 4799
NdeI CATATG 3 cut(s) 298, 3642, 3726
NgoMIV GCCGGC 1 cut(s) 50
NlaIV GGNNCC 8 cut(s) 469, 636, 677, 1968, 2223, 2800, 2914, 4210
NspI RCATGY 8 cut(s) 564, 3316, 3400, 3484, 3562, 3814, 3898, 3982
PaqCI CACCTGC 1 cut(s) 3020
PciI ACATGT 7 cut(s) 3312, 3396, 3480, 3558, 3810, 3894, 3978
PciSI GCTCTTC 1 cut(s) 1100
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 135
PctI GAATGC 2 cut(s) 2077, 2104
PdiI GCCGGC 1 cut(s) 52
PdmI GAANNNNTTC 4 cut(s) 1757, 3371, 3455, 4034
Ple19I CGATCG 1 cut(s) 30
PpuMI RGGWCCY 1 cut(s) 1205
PscI ACATGT 7 cut(s) 3312, 3396, 3480, 3558, 3810, 3894, 3978
Psp5II RGGWCCY 1 cut(s) 1205
Psp6I CCWGG 7 cut(s) 2240, 2375, 2513, 2636, 2759, 3152, 4739
PspFI CCCAGC 8 cut(s) 993, 2042, 2283, 2421, 2559, 2682, 3018, 3036
PspGI CCWGG 7 cut(s) 2240, 2375, 2513, 2636, 2759, 3152, 4739
PspN4I GGNNCC 8 cut(s) 469, 636, 677, 1968, 2223, 2800, 2914, 4210
PspOMI GGGCCC 1 cut(s) 675
PspPI GGNCC 6 cut(s) 675, 676, 1205, 1508, 1820, 4292
PspPPI RGGWCCY 1 cut(s) 1205
PsrI GAACNNNNNNTAC 2 cut(s) 588, 620
PstI CTGCAG 1 cut(s) 480
PstNI CAGNNNCTG 5 cut(s) 1465, 2363, 2501, 2621, 2744
PsuI RGATCY 4 cut(s) 373, 1385, 1966, 2912
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 2882
RgaI GCGATCGC 1 cut(s) 30
RseI CAYNNNNRTG 3 cut(s) 1478, 4428, 4491
SapI GCTCTTC 1 cut(s) 1100
SaqAI TTAA 9 cut(s) 945, 986, 1494, 1805, 1826, 1878, 1911, 1986, 3291
Sau96I GGNCC 6 cut(s) 675, 676, 1205, 1508, 1820, 4292
ScaI AGTACT 1 cut(s) 2962
SfaAI GCGATCGC 1 cut(s) 30
SfaNI GCATC 9 cut(s) 426, 1119, 1930, 2363, 2501, 2600, 2621, 2744, 2959
SfcI CTRYAG 3 cut(s) 180, 476, 1546
SgfI GCGATCGC 1 cut(s) 30
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 4 cut(s) 1205, 1508, 1820, 4292
SmiMI CAYNNNNRTG 3 cut(s) 1478, 4428, 4491
SmlI CTYRAG 4 cut(s) 356, 863, 944, 3048
SmoI CTYRAG 4 cut(s) 356, 863, 944, 3048
SspI AATATT 1 cut(s) 1560
StyI CCWWGG 3 cut(s) 1736, 4799, 4883
TaiI ACGT 3 cut(s) 552, 1650, 2220
TaqI TCGA 2 cut(s) 8, 96
TaqII GACCGA 1 cut(s) 1784
TatI WGTACW 2 cut(s) 1426, 2960
TauI GCSGC 9 cut(s) 57, 110, 2528, 2651, 3669, 3753, 3837, 3921, 4005
Tru1I TTAA 9 cut(s) 945, 986, 1494, 1805, 1826, 1878, 1911, 1986, 3291
Tru9I TTAA 9 cut(s) 945, 986, 1494, 1805, 1826, 1878, 1911, 1986, 3291
TscAI CASTG 6 cut(s) 658, 1123, 2593, 2716, 4129, 4240
TspGWI ACGGA 2 cut(s) 1794, 2791
TspRI CASTG 6 cut(s) 658, 1123, 2593, 2716, 4129, 4240
Vha464I CTTAAG 1 cut(s) 944
VpaK11BI GGWCC 4 cut(s) 1205, 1508, 1820, 4292
XagI CCTNNNNNAGG 2 cut(s) 1740, 3195
XapI RAATTY 6 cut(s) 254, 794, 1028, 1442, 1809, 2128
XceI RCATGY 8 cut(s) 564, 3316, 3400, 3484, 3562, 3814, 3898, 3982
XcmI CCANNNNNNNNNTGG 1 cut(s) 1734
XmiI GTMKAC 1 cut(s) 1545
XmnI GAANNNNTTC 4 cut(s) 1757, 3371, 3455, 4034
ZrmI AGTACT 1 cut(s) 2962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.