FvH4_7g24750

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0018730
Physical Location & Seq
Reverse (-)
19059572 .. 19060669
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24750.t1

Sequence Viewer

Length: 489 bp
ATGTCGACTCCAGATTCTTCCGGAATCTTTCACGGCCACTACACCGTCCTCGACGACAAAGAGTTCCAAGTCAAGGGCCGAACCCTCTTCTTCGTCGTGATCCTCTTCACAGTCGTCCTCCTCGTCACCGTCTTCTTGCTCTACACCAGATGGGTCTGCCGCCGCCGCCGCCGCCCCTCCAACTCATCTTCCTCCTCCGAAGCGCCCCCGCAGAAGCCGCGCGGCCTCGACGCTGCCCTGATTCGAGGTTTGCCTATAATATTGTATCATTCCTCCGGCGACTCCGACGTGGAAGCAGGATGCGGTGAGTGTTCTATATGTCTCGGAGTTTTCGAAGACGGGGAGAAGGTTAAAGTTCTCCCTCAGTGCCGCCATTGCTACCACTCCGACTGCGTCGATACATGGCTCAGCGCTCAGTCCAGTTGCCCCCTCTGCCGCACGTCGCTCCAGTCAAACACCGACGACGCTCTGCAAATCATCGTACAGTGA

Protein Analysis

163

Amino Acids

18.0

Weight (kDa)

6.49

Isoelectric Point (pI)

63.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 103 - 146 1.7e-14 Ring finger domain
zf-RING_11 PF17123 103 - 132 1.9e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 103 - 145 3.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 104 - 145 7.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 112 - 146 2.3e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018730)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24750
rosa_chinensis RchiOBHm_Chr1g0370601
rosa_laevigata RLG00000027024
rosa_multiflora Rmu_sc0002021.1_g000012
rosa_roxburghii Rroxscaffold_4G00286400
rosa_rugosa Rorug01G0358000
rosa_samantha Rh1BG330700 Rh1DG362200
rosa_wichuraiana Rw1G032330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 220, 222
AccIII TCCGGA 1 cut(s) 20
AclWI GGATC 1 cut(s) 94
AcoI YGGCCR 1 cut(s) 34
AfaI GTAC 1 cut(s) 483
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 73
AjiI CACGTC 2 cut(s) 289, 441
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 1 cut(s) 94
Aor13HI TCCGGA 1 cut(s) 20
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 3 cut(s) 34, 76, 223
ApeKI GCWGC 1 cut(s) 233
AspLEI GCGC 3 cut(s) 205, 222, 413
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 118, 317
AsuII TTCGAA 1 cut(s) 333
BbsI GAAGAC 2 cut(s) 124, 342
BbvI GCAGC 1 cut(s) 220
BccI CCATC 1 cut(s) 144
BceAI ACGGC 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 326
BfoI RGCGCY 2 cut(s) 206, 414
BlpI GCTNAGC 1 cut(s) 407
BmgBI CACGTC 2 cut(s) 289, 441
BmgT120I GGNCC 1 cut(s) 76
BmsI GCATC 1 cut(s) 290
BpiI GAAGAC 2 cut(s) 124, 342
BpmI CTGGAG 1 cut(s) 431
Bpu1102I GCTNAGC 1 cut(s) 407
Bpu14I TTCGAA 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 420, 448
Bse3DI GCAATG 1 cut(s) 373
BseAI TCCGGA 1 cut(s) 20
BseGI GGATG 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 3 cut(s) 377, 421, 428
BseNI ACTGG 2 cut(s) 420, 448
BseRI GAGGAG 2 cut(s) 110, 184
BseXI GCAGC 1 cut(s) 220
Bsh1236I CGCG 2 cut(s) 220, 222
BshFI GGCC 3 cut(s) 36, 78, 225
BsiSI CCGG 2 cut(s) 21, 276
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 326
BsnI GGCC 3 cut(s) 36, 78, 225
Bsp119I TTCGAA 1 cut(s) 333
Bsp13I TCCGGA 1 cut(s) 20
Bsp143I GATC 1 cut(s) 99
Bsp1720I GCTNAGC 1 cut(s) 407
BspANI GGCC 3 cut(s) 36, 78, 225
BspCNI CTCAG 3 cut(s) 376, 420, 427
BspEI TCCGGA 1 cut(s) 20
BspFNI CGCG 2 cut(s) 220, 222
BspPI GGATC 1 cut(s) 94
BspT104I TTCGAA 1 cut(s) 333
BsrDI GCAATG 1 cut(s) 373
BsrI ACTGG 2 cut(s) 420, 448
BssMI GATC 1 cut(s) 99
Bst4CI ACNGT 4 cut(s) 46, 112, 130, 486
Bst6I CTCTTC 2 cut(s) 92, 110
BstBI TTCGAA 1 cut(s) 333
BstDEI CTNAG 3 cut(s) 363, 407, 414
BstF5I GGATG 1 cut(s) 305
BstFNI CGCG 2 cut(s) 220, 222
BstH2I RGCGCY 2 cut(s) 206, 414
BstHHI GCGC 3 cut(s) 205, 222, 413
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 6 cut(s) 165, 168, 171, 217, 375, 432
BstUI CGCG 2 cut(s) 220, 222
BstV1I GCAGC 1 cut(s) 220
BstV2I GAAGAC 2 cut(s) 124, 342
BsuRI GGCC 3 cut(s) 36, 78, 225
BtrI CACGTC 2 cut(s) 289, 441
BtsCI GGATG 1 cut(s) 305
BtsIMutI CAGTG 1 cut(s) 371
CfoI GCGC 3 cut(s) 205, 222, 413
Cfr13I GGNCC 1 cut(s) 76
CseI GACGC 3 cut(s) 239, 382, 473
Csp6I GTAC 1 cut(s) 482
CviAII CATG 1 cut(s) 402
CviJI RGCY 5 cut(s) 36, 78, 217, 225, 406
CviKI_1 RGCY 5 cut(s) 36, 78, 217, 225, 406
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 3 cut(s) 363, 407, 414
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 92, 110
EarI CTCTTC 2 cut(s) 92, 110
Eco47III AGCGCT 1 cut(s) 412
FaeI CATG 1 cut(s) 405
FaiI YATR 4 cut(s) 257, 317, 319, 403
FatI CATG 1 cut(s) 401
FauI CCCGC 1 cut(s) 216
FblI GTMKAC 1 cut(s) 5
FokI GGATG 1 cut(s) 312
GlaI GCGC 3 cut(s) 204, 221, 412
GsuI CTGGAG 1 cut(s) 431
HaeII RGCGCY 2 cut(s) 206, 414
HaeIII GGCC 3 cut(s) 36, 78, 225
HapII CCGG 2 cut(s) 21, 276
HgaI GACGC 3 cut(s) 239, 382, 473
HhaI GCGC 3 cut(s) 205, 222, 413
Hin1II CATG 1 cut(s) 405
Hin6I GCGC 3 cut(s) 203, 220, 411
HinP1I GCGC 3 cut(s) 203, 220, 411
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 5 cut(s) 7, 14, 24, 241, 281
HpaII CCGG 2 cut(s) 21, 276
HphI GGTGA 2 cut(s) 118, 317
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 199, 286, 326, 388
Hpy188III TCNNGA 3 cut(s) 11, 21, 97
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 8 cut(s) 56, 98, 233, 290, 398, 445, 464, 467
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 4 cut(s) 46, 112, 130, 486
HpyCH4IV ACGT 2 cut(s) 288, 440
HpyCH4V TGCA 1 cut(s) 472
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 168, 171, 217, 375, 432
HpyF3I CTNAG 3 cut(s) 363, 407, 414
HpySE526I ACGT 2 cut(s) 288, 440
Hsp92II CATG 1 cut(s) 405
HspAI GCGC 3 cut(s) 203, 220, 411
Kpn2I TCCGGA 1 cut(s) 20
Kzo9I GATC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 450
LpnPI CCDG 8 cut(s) 24, 34, 160, 251, 282, 289, 433, 461
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 1 cut(s) 290
MaeII ACGT 2 cut(s) 288, 440
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 7 cut(s) 9, 79, 82, 97, 124, 180, 347
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 3 cut(s) 204, 309, 411
MroI TCCGGA 1 cut(s) 20
MseI TTAA 1 cut(s) 351
MspI CCGG 2 cut(s) 21, 276
MvnI CGCG 2 cut(s) 220, 222
MwoI GCNNNNNNNGC 6 cut(s) 165, 168, 171, 217, 375, 432
NdeII GATC 1 cut(s) 99
NlaIII CATG 1 cut(s) 405
NmuCI GTSAC 1 cut(s) 124
NspV TTCGAA 1 cut(s) 333
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 330
PfeI GAWTC 3 cut(s) 14, 24, 241
PflFI GACNNNGTC 1 cut(s) 392
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
PspPI GGNCC 1 cut(s) 76
PsyI GACNNNGTC 1 cut(s) 392
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 351
Sau3AI GATC 1 cut(s) 99
Sau96I GGNCC 1 cut(s) 76
SchI GAGTC 1 cut(s) 275
SetI ASST 4 cut(s) 250, 291, 351, 443
SfaNI GCATC 1 cut(s) 290
SfuI TTCGAA 1 cut(s) 333
SspI AATATT 1 cut(s) 261
TaaI ACNGT 4 cut(s) 46, 112, 130, 486
TaiI ACGT 2 cut(s) 291, 443
TaqI TCGA 6 cut(s) 5, 51, 228, 244, 333, 396
TauI GCSGC 9 cut(s) 162, 165, 168, 171, 174, 220, 225, 372, 438
TfiI GAWTC 3 cut(s) 14, 24, 241
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 371
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 124
TspRI CASTG 1 cut(s) 371
Tth111I GACNNNGTC 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.