Rorug01G0358000

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014271
Physical Location & Seq
Forward (+)
47412078 .. 47420093
8016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0358000.1

Sequence Viewer

Length: 4230 bp
ATGATGATCAATCGGGGGCTGTTCGGGTGGTCCCCCCCGCACGTACAGCCGCTGACTCCGGTGTCTGAGGTCTCGGAGCCGCCGGAGTCGCCGTCGCCGTACATGGACATGAGCGGGGACACGTCGGCGCAGCCGGTCGAGCAGGAGGAGGAGATGGAGGAGCCTGAGGAGATCGAGCCGCCGCCCGCTGCCGTGCCGTTTACGAGGCTGTTCACCTGCGCCGACCGCCTCGATTGGGTTTTGATGACCGTCGGATCGCTGGCGGCCGCCGCGCACGGCACGGCGCTCGTGGTGTACTTGCACTTTTTTGCTAAGATTATTCATGTGCTTTGGATCGAAAAGGATTCTAAAGAAGCGCCGCCGATGCATGTGACGCCGGAGCAGTACCAGAAGTTTATGGATCTTGCTTTAAGCATTGTTTATATCGCTGTGGGTGTTTTTGCTGCTGGTTGGATTGAGGTCTCATGCTGGATTCTGACTGGAGAACGGCAGACTGCTGTCATCAGGTCAAGATATGTTCAAGTATTACTTAACCAGGATATGAGTTTTTTTGATACCTATGGGAACAATGGGGACATCGTGAGCCAAGTTTTGAGTGATGTTCTACTCATTCAGTCTGCTCTCAGTGAAAAAGTTGGAAATTATATTCATAACATGGCGACATTTTTCAGTGGGCTCATTATTGGATTCATCAACTGTTGGCAGATTGCGGCTATAACATTAGCCACTGGTCCATTTATTGTTGCTGCTGGAGGAATATCAAATATATTTCTTCACAGGCTTGCTGAGAATATTCAAGATGCATATGCTGAAGCAGCTAGCATTGCTGAACAGGCAGTCTCTTACATTGGGACATTATATGCATTTACAAATGAAACATTGGCCAAGTATTCTTATGCAACATCACTACAAGCAACTCTTCGATATGGTATATTGATTAGTCTGGTGCAAGGACTAGGACTTGGATTTACATATGGGCTTGCCATTTGTTCATGTGCCTTGCAACTCTGGGTTGGAAGGTTCCTGGTTACTCATGAAAAAGCTCATGGTGGTGAAATTATAACAGCTTTATTTGCTGTAATATTAAGTGGCCTTGGGCTGAACCAAGCAGCAACAAACTTCTACTCATTTGATCAAGGTCGAATTGCGGCTTATAGACTGTTTGAGATGATACAAAGATCATCCTCTAATGTTAATCACGAGGGAACTACCCTAGTGGCTGTACAAGGAAATATTGAGTTCCGGAATGTGTATTTCAGTTATCCATCTCGTCCGGAGATTCCTATTTTGAGTAGTTTTTACCTCTCTGTACCTGCTAAGAAAGCTGTGGCACTCGTGGGTAGAAATGGATCTGGAAAGAGCAGTATCATTCCCCTCATGGAGCAGTTTTATAATCCAACATTAGGAGAAGTTCTTTTGGATGGAGAAAATATAAAAAACCTGAATTTGGAATGGCTCAGAAGCCAGATAGGGCTAGTCACACAAGATCCTGCCTTGTTAAGTTTGAGTATAAGAGATAATATTGCTTATGGGCGGGATGCGACAATGGATCAAATTGAAGAAGCTGCTAAAATAGCACATGCACATACATTTATTACTTCGCTTGAGAGATCGTATGAAACACAGGTAGGAAGGGCAGGATTAGCACTGACAGAAGAGCAAAAAATAAAACTTTCAATTGCCAGGGCAGTGCTTCTGAATCCATCAATTCTTCTGCTTGATGAGGTCACTGGTGGACTTGATTTTGAAGCTGAAAGAACTGTTCAAGAGGCTCTAGATCTCCTCATGTTGGGACGTTCGACTATAATTATAGCTCGGCGGCTTAGTCTTATAAGGAATGCTGATTACATAGCTGTGATGGAGGAAGGTCAACTAGTTGAAACGGGTACACATGAAGAGTTAATAACCCATGATGGCCTATATGCAGAGCTTCTCAGGTGTGAAGAAGCTGCAAAGCTTCCCCGGAGAATGCCAGTGCGGAATTACAAGGAGACTACAGCTTTCCAAATTGAAATGGATTCTTCAGCAAGTAATAGCTTCCAAGAACCATCATCCCCAAAAATGATGAAATCACCCTCACTTCAGAGGGTTACTGGTATATTTCGAATGGGTGATGTCAATTTTAATGCACAGGACTCACCAAAACCTAAGAGTCCACCAGTAGAGAAAGTGGTGGAAAACGGTCAGCAGCCCTTGGATACAGCAGATAAGGAACCATCAATAAAGCGGCAGGATAGTTTTGAAATGAGACTACCAGAGCTACCCAAGATTGATGTCCAGTCTGCAAATCAACAAACAACAAATGGTTCGGACCCTGAATCCCCTGTTTCACCCCTTTTGACATCTGATCCGAAAAATGAACGTTCTCATTCACAAACCTTTAGCCGCCCTCATAGCCATTCTGATGATGTTCCCATCAAAGTGAAGGGAGCAAAGAGTACACATTATAAAAAGTCACCATCATTTTGGAGGCTTGCACAGCTTTCTTTTGCAGAGTGGCTTTATGCTGTGTTAGGAAGCATTGGTGCTGCTATCTTTGGTTCCTTTAATCCTCTTCTTGCTTATGTTATCGCACTTGTAGTAACAGCATACTACAGGGGTAGTGAAGGTCGTCACTTGAGGCAAGAAGTAGACAAATGGTGCTTGATCATTGCTTGCATGGGTATAGTGACAGTTGTTGCCAATTTCTTGCAACACTTCTACTTTGGTATCATGGGGGAGAAAATGACTGAACGAGTTCGCAGAATGATGTTTTCAGCAATGCTCCGCAATGAGGTGGGATGGTTTGATGAAGAGGAAAACAGTGCTGATACCTTGTCCATGCGCTTGGCAAATGATGCTACATTTGTGCGAGCTGCTTTTAGTAATCGGCTTTCAATATTCATACAGGATAGTGCTGCTATCATTGTGGCTGTTTTGATTGGGATGTTGCTACAATGGCGATTAGCACTTGTGGCTTTGGCAACCCTACCTGTTCTCACTATTTCTGCCATTGCACAGAAATTATGGCTTGCTGGTTTTTCAAGGGGGATTCAGGAGATGCACAGGAAAGCATCTTTGGTTCTTGAGGATGCAGTTAGAAACATTTACACTGTGGTAGCATTCTGTGCTGGTAACAAGGTAATGGAGCTCTACAGATTGCAGCTAAAGAAGATCTTTAAGCAGAGTTTCTTTCATGGAATGGCCATCGGGTTTGCATTTGGTTTTTCACAGTTCCTTCTTTTTGCCTGCAACGCTCTTCTCCTCTGGTACACTGCATACTCCGTGAAACAGAAGTATATGGATCTACCTACTGCTCTCAAGGAGTATATGGTTTTCTCTTTTGCAACATTCGCACTGGTAGAGCCCTTTGGGTTGGCTCCTTACATACTCAAACGACGTAAATCTCTCATTTCAGTATTTGAAATCATAGATCGAGTGCCAAAGATTGAGCCAGATGAAAGCTCCGCCATGAAACCACCTAATGTTTATGGAAGCCTTGAGTTGAAAAATGTTGATTTCTGTTATCCAACCCGCCCAGAACTGTTGGTGCTGAGCAATTTCAGTCTCAAAGTAAACGGGGGGCAAACTGTAGCTGTGGTGGGAGTTTCAGGGTCGGGTAAGAGCACTATAATTTCTTTGATCGAGAGATTTTATGATCCAGTTGCTGGTCAAGTGATGCTGGATGGTCGAGATCTGAAGCTTTATAATTTGAGATGGTTGAGGAACCACCTTGGTCTAGTTCAACAGGAGCCCATTATTTTCTCGACAACAATAAGGGAGAATATAATATATGCAAGGCATAATGCTAGTGAAGCTGAAATGAAAGAGGCTGCAAGAATAGCGAATGCTCATCATTTCATCAGCAGCTTGCCTCATGGTTATGATACACATGTGGGGATGAGAGGGGTTGATCTAACCCCAGGACAGAAGCAAAGAATTGCAATCGCTCGTGTTGTTTTAAAAAATGCTCCCATTTTGTTATTGGATGAAGCAAGTTCGTCTATTGAATCTGAGTCAAGTCGAGTGGTGCAGGAGGCCCTTGATACATTAATCATGGGGAACAAAACAACCATTTTGATAGCTCACAGAGCTGCAATGATGAGGCACGTTGACAACATTGTAGTTCTTAATGGAGGGAGAATTGTGGAAGAAGGGTCCCATGATTCTTTAATGTCAAAGAACGGTCTGTATGTCCGTTTGATGCATCCTCACTTTGGCAAGGGTATGCGTCAGCACCGCCTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000272 GO:0001067 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009755 GO:0009756 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009987 GO:0010033 GO:0010035 GO:0010119 GO:0010154 GO:0010182 GO:0010353 GO:0010449 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010896 GO:0016052 GO:0019216 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031930 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032880 GO:0033993 GO:0034284 GO:0034285 GO:0035266 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044042 GO:0044212 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048316 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0050994 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080090 GO:0090207 GO:0090696 GO:0097159 GO:0097305 GO:0097306 GO:0099402 GO:0140110 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

1409

Amino Acids

156.61

Weight (kDa)

6.18

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 86 - 361 2.3e-39 ABC transporter transmembrane region
ABC_tran PF00005 433 - 577 4.9e-22 ABC transporter
ABC_membrane PF00664 841 - 1100 1.9e-39 ABC transporter transmembrane region
ABC_tran PF00005 1178 - 1326 1.9e-28 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40220
fragaria_vesca FvH4_7g24760
malus_domestica MD01G1155400.v1.1 MD07G1224400.v1.1
prunus_persica Prupe.2G253000_v2.0.a1
pyrus_communis pycom01g17270 pycom13g15020
rosa_chinensis RchiOBHm_Chr1g0370631
rosa_laevigata RLG00000027023
rosa_multiflora Rmu_sc0002021.1_g000011
rosa_roxburghii Rroxscaffold_4G00286390
rosa_rugosa Rorug01G0358000
rosa_samantha Rh1AG367500 Rh1BG330800 Rh1CG345000 Rh1DG362300
rosa_wichuraiana Rw1G032340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1061, 1392, 1832, 2448, 3687
AarI CACCTGC 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 61
Acc36I ACCTGC 2 cut(s) 224, 1321
AccB7I CCANNNNNTGG 1 cut(s) 3647
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 2631
AccII CGCG 1 cut(s) 272
AccIII TCCGGA 2 cut(s) 1242, 1273
AclI AACGTT 1 cut(s) 2362
AclWI GGATC 9 cut(s) 262, 341, 408, 1357, 1481, 1557, 2342, 3291, 3632
AcoI YGGCCR 3 cut(s) 264, 882, 3183
AcsI RAATTY 1 cut(s) 1444
AcuI CTGAAG 4 cut(s) 831, 2007, 2066, 3698
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 9 cut(s) 45, 101, 296, 386, 1224, 1311, 1888, 2440, 3251
AfiI CCNNNNNNNGG 7 cut(s) 235, 1403, 1447, 1789, 2773, 3647, 4196
AflIII ACRYGT 2 cut(s) 120, 3871
AhlI ACTAGT 1 cut(s) 1873
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 4 cut(s) 534, 1023, 1682, 3901
AjuI GAANNNNNNNTTGG 2 cut(s) 3041, 3073
Alw21I GWGCWC 2 cut(s) 3132, 3608
Alw26I GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
AlwI GGATC 9 cut(s) 262, 341, 408, 1357, 1481, 1557, 2342, 3291, 3632
AlwNI CAGNNNCTG 2 cut(s) 52, 3647
Aor13HI TCCGGA 2 cut(s) 1242, 1273
AoxI GGCC 6 cut(s) 264, 882, 1090, 1915, 3183, 4017
ApoI RAATTY 1 cut(s) 1444
AseI ATTAAT 1 cut(s) 4031
Asp700I GAANNNNTTC 1 cut(s) 2736
AspLEI GCGC 6 cut(s) 130, 221, 274, 286, 358, 2826
AspS9I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4137
AsuC2I CCSGG 1 cut(s) 1963
AsuHPI GGTGA 7 cut(s) 205, 1064, 2064, 2123, 2130, 2322, 2448
AsuII TTCGAA 1 cut(s) 2104
AsuNHI GCTAGC 1 cut(s) 818
AvaII GGWCC 4 cut(s) 30, 731, 2311, 4137
AxyI CCTNAGG 1 cut(s) 165
BaeI ACNNNNGTAYC 4 cut(s) 2692, 2725, 3858, 3891
BalI TGGCCA 2 cut(s) 884, 3185
BanII GRGCYC 4 cut(s) 678, 3132, 3348, 3735
BauI CACGAG 4 cut(s) 287, 1199, 1334, 3930
Bbv12I GWGCWC 2 cut(s) 3132, 3608
BceAI ACGGC 7 cut(s) 76, 82, 176, 181, 292, 297, 503
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 4 cut(s) 536, 1025, 1684, 3903
BciVI GTATCC 1 cut(s) 2191
BclI TGATCA 3 cut(s) 6, 1132, 2646
BcnI CCSGG 1 cut(s) 1963
BcoDI GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
BcuI ACTAGT 1 cut(s) 1873
BfaI CTAG 8 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3719, 3789
BfmI CTRYAG 4 cut(s) 1995, 2593, 3133, 3570
BfoI RGCGCY 2 cut(s) 287, 359
BfuAI ACCTGC 2 cut(s) 224, 1321
BfuI GTATCC 1 cut(s) 2191
BglII AGATCT 3 cut(s) 1777, 3153, 3673
BlpI GCTNAGC 1 cut(s) 3533
Bme1390I CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
Bme18I GGWCC 4 cut(s) 30, 731, 2311, 4137
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4137
BmrFI CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
BmtI GCTAGC 1 cut(s) 822
BoxI GACNNNNGTC 1 cut(s) 497
BpmI CTGGAG 2 cut(s) 501, 771
Bpu1102I GCTNAGC 1 cut(s) 3533
Bpu14I TTCGAA 1 cut(s) 2104
BpuEI CTTGAG 5 cut(s) 1625, 2638, 3086, 3284, 3500
BpuMI CCSGG 1 cut(s) 1963
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 374
BsaI GGTCTC 2 cut(s) 76, 466
BsaJI CCNNGG 6 cut(s) 1093, 1683, 1961, 2193, 3712, 3901
BsaWI WCCGGW 3 cut(s) 58, 1242, 1273
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 7 cut(s) 235, 1403, 1447, 1789, 2773, 3647, 4196
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 9 cut(s) 484, 733, 1735, 1973, 2098, 2159, 2278, 3342, 3641
Bse21I CCTNAGG 1 cut(s) 165
Bse3DI GCAATG 6 cut(s) 822, 2649, 2766, 2776, 2991, 4083
BseAI TCCGGA 2 cut(s) 1242, 1273
BseBI CCWGG 4 cut(s) 536, 1025, 1684, 3903
BseDI CCNNGG 6 cut(s) 1093, 1683, 1961, 2193, 3712, 3901
BseLI CCNNNNNNNGG 7 cut(s) 235, 1403, 1447, 1789, 2773, 3647, 4196
BseMI GCAATG 6 cut(s) 822, 2649, 2766, 2776, 2991, 4083
BseMII CTCAG 8 cut(s) 57, 156, 637, 777, 1471, 1948, 3524, 3984
BseNI ACTGG 9 cut(s) 484, 733, 1735, 1973, 2098, 2159, 2278, 3342, 3641
BseRI GAGGAG 6 cut(s) 161, 164, 173, 182, 1772, 3233
BseX3I CGGCCG 1 cut(s) 264
BsgI GTGCAG 1 cut(s) 4031
Bsh1236I CGCG 1 cut(s) 272
Bsh1285I CGRYCG 3 cut(s) 138, 226, 267
BshFI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
BsiEI CGRYCG 3 cut(s) 138, 226, 267
BsiHKAI GWGCWC 2 cut(s) 3132, 3608
BsiSI CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 1963
BslFI GGGAC 6 cut(s) 16, 131, 587, 865, 1806, 4123
BslI CCNNNNNNNGG 7 cut(s) 235, 1403, 1447, 1789, 2773, 3647, 4196
BsmAI GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
BsmFI GGGAC 6 cut(s) 16, 131, 587, 865, 1806, 4123
BsmI GAATGC 4 cut(s) 1843, 1974, 3101, 3832
BsnI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
Bso31I GGTCTC 2 cut(s) 76, 466
Bsp119I TTCGAA 1 cut(s) 2104
Bsp1286I GDGCHC 5 cut(s) 678, 3132, 3348, 3608, 3735
Bsp13I TCCGGA 2 cut(s) 1242, 1273
Bsp1407I TGTACA 1 cut(s) 1222
Bsp1720I GCTNAGC 1 cut(s) 3533
BspANI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
BspCNI CTCAG 8 cut(s) 58, 157, 636, 778, 1470, 1947, 3525, 3985
BspEI TCCGGA 2 cut(s) 1242, 1273
BspFNI CGCG 1 cut(s) 272
BspHI TCATGA 1 cut(s) 1033
BspMI ACCTGC 2 cut(s) 224, 1321
BspOI GCTAGC 1 cut(s) 822
BspPI GGATC 9 cut(s) 262, 341, 408, 1357, 1481, 1557, 2342, 3291, 3632
BspQI GCTCTTC 2 cut(s) 1650, 3243
BspT104I TTCGAA 1 cut(s) 2104
BspTNI GGTCTC 2 cut(s) 76, 466
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 6 cut(s) 822, 2649, 2766, 2776, 2991, 4083
BsrFI RCCGGY 1 cut(s) 133
BsrGI TGTACA 1 cut(s) 1222
BsrI ACTGG 9 cut(s) 484, 733, 1735, 1973, 2098, 2159, 2278, 3342, 3641
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 6 cut(s) 1093, 1683, 1961, 2193, 3712, 3901
BssNI GRCGYC 1 cut(s) 374
BssSI CACGAG 4 cut(s) 287, 1199, 1334, 3930
BssT1I CCWWGG 3 cut(s) 1093, 2193, 3712
Bst2BI CACGAG 4 cut(s) 287, 1199, 1334, 3930
Bst2UI CCWGG 4 cut(s) 536, 1025, 1684, 3903
Bst6I CTCTTC 6 cut(s) 924, 1650, 1890, 2559, 2787, 3243
BstACI GRCGYC 1 cut(s) 374
BstAPI GCANNNNNTGC 2 cut(s) 2837, 3107
BstAUI TGTACA 1 cut(s) 1222
BstBAI YACGTR 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 2104
BstFNI CGCG 1 cut(s) 272
BstH2I RGCGCY 2 cut(s) 287, 359
BstHHI GCGC 6 cut(s) 130, 221, 274, 286, 358, 2826
BstMAI GTCTC 6 cut(s) 76, 466, 844, 1985, 2242, 3551
BstMCI CGRYCG 3 cut(s) 138, 226, 267
BstNI CCWGG 4 cut(s) 536, 1025, 1684, 3903
BstNSI RCATGY 3 cut(s) 371, 1583, 3875
BstPAI GACNNNNGTC 1 cut(s) 497
BstSCI CCNGG 5 cut(s) 534, 1023, 1682, 1961, 3901
BstSFI CTRYAG 4 cut(s) 1995, 2593, 3133, 3570
BstUI CGCG 1 cut(s) 272
BstX2I RGATCY 7 cut(s) 400, 1349, 1486, 1777, 3153, 3283, 3673
BstXI CCANNNNNNTGG 2 cut(s) 2466, 2827
BstYI RGATCY 7 cut(s) 400, 1349, 1486, 1777, 3153, 3283, 3673
BstZI CGGCCG 1 cut(s) 264
Bsu36I CCTNAGG 1 cut(s) 165
BsuI GTATCC 1 cut(s) 2191
BsuRI GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
BtrI CACGTC 1 cut(s) 123
BtsI GCAGTG 2 cut(s) 1695, 3252
BveI ACCTGC 2 cut(s) 224, 1321
CaiI CAGNNNCTG 2 cut(s) 52, 3647
CciI TCATGA 1 cut(s) 1033
CciNI GCGGCCGC 1 cut(s) 264
CfoI GCGC 6 cut(s) 130, 221, 274, 286, 358, 2826
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4137
CseI GACGC 2 cut(s) 382, 4199
Csp6I GTAC 9 cut(s) 44, 100, 295, 385, 1223, 1310, 1887, 2439, 3250
CspCI CAANNNNNGTGG 2 cut(s) 3987, 4022
CviQI GTAC 9 cut(s) 44, 100, 295, 385, 1223, 1310, 1887, 2439, 3250
DraI TTTAAA 1 cut(s) 3942
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 3 cut(s) 264, 882, 3183
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 6 cut(s) 924, 1650, 1890, 2559, 2787, 3243
EarI CTCTTC 6 cut(s) 924, 1650, 1890, 2559, 2787, 3243
EciI GGCGGA 1 cut(s) 3436
Ecl136II GAGCTC 1 cut(s) 3130
EclXI CGGCCG 1 cut(s) 264
Eco130I CCWWGG 3 cut(s) 1093, 2193, 3712
Eco24I GRGCYC 4 cut(s) 678, 3132, 3348, 3735
Eco31I GGTCTC 2 cut(s) 76, 466
Eco47I GGWCC 4 cut(s) 30, 731, 2311, 4137
Eco52I CGGCCG 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 3130
Eco57I CTGAAG 4 cut(s) 831, 2007, 2066, 3698
Eco81I CCTNAGG 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 3130
EcoO109I RGGNCCY 2 cut(s) 4018, 4137
EcoRII CCWGG 4 cut(s) 534, 1023, 1682, 3901
EcoT14I CCWWGG 3 cut(s) 1093, 2193, 3712
EcoT22I ATGCAT 4 cut(s) 369, 805, 865, 4188
EcoT38I GRGCYC 4 cut(s) 678, 3132, 3348, 3735
ErhI CCWWGG 3 cut(s) 1093, 2193, 3712
FalI AAGNNNNNCTT 6 cut(s) 513, 545, 903, 935, 3140, 3172
FaqI GGGAC 6 cut(s) 16, 131, 587, 865, 1806, 4123
FauI CCCGC 5 cut(s) 45, 107, 193, 1527, 3521
FauNDI CATATG 2 cut(s) 805, 973
FbaI TGATCA 3 cut(s) 6, 1132, 2646
FblI GTMKAC 1 cut(s) 2631
FriOI GRGCYC 4 cut(s) 678, 3132, 3348, 3735
FspBI CTAG 8 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3719, 3789
GlaI GCGC 6 cut(s) 129, 220, 273, 285, 357, 2825
GsuI CTGGAG 2 cut(s) 501, 771
HaeII RGCGCY 2 cut(s) 287, 359
HaeIII GGCC 6 cut(s) 266, 884, 1092, 1917, 3185, 4019
HapII CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 1963
HgaI GACGC 2 cut(s) 382, 4199
HhaI GCGC 6 cut(s) 130, 221, 274, 286, 358, 2826
Hin1I GRCGYC 1 cut(s) 374
Hin6I GCGC 6 cut(s) 128, 219, 272, 284, 356, 2824
HinP1I GCGC 6 cut(s) 128, 219, 272, 284, 356, 2824
HincII GTYRAC 2 cut(s) 1871, 4093
HindII GTYRAC 2 cut(s) 1871, 4093
HindIII AAGCTT 2 cut(s) 1955, 3680
HpaII CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 1963
HphI GGTGA 7 cut(s) 205, 1064, 2064, 2123, 2130, 2322, 2448
Hpy99I CGWCG 4 cut(s) 97, 127, 254, 3381
HpyAV CCTTC 7 cut(s) 1011, 1626, 1859, 2419, 2600, 3227, 4127
HpyCH4IV ACGT 6 cut(s) 42, 122, 1795, 2362, 3379, 4089
HpySE526I ACGT 6 cut(s) 42, 122, 1795, 2362, 3379, 4089
Hsp92I GRCGYC 1 cut(s) 374
HspAI GCGC 6 cut(s) 128, 219, 272, 284, 356, 2824
KflI GGGWCCC 1 cut(s) 4137
Kpn2I TCCGGA 2 cut(s) 1242, 1273
Ksp22I TGATCA 3 cut(s) 6, 1132, 2646
LguI GCTCTTC 2 cut(s) 1650, 3243
MaeI CTAG 8 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3719, 3789
MaeII ACGT 6 cut(s) 42, 122, 1795, 2362, 3379, 4089
MbiI CCGCTC 1 cut(s) 114
MfeI CAATTG 1 cut(s) 1677
MflI RGATCY 7 cut(s) 400, 1349, 1486, 1777, 3153, 3283, 3673
MhlI GDGCHC 5 cut(s) 678, 3132, 3348, 3608, 3735
MlsI TGGCCA 2 cut(s) 884, 3185
MluNI TGGCCA 2 cut(s) 884, 3185
MlyI GAGTC 5 cut(s) 49, 95, 2129, 2161, 4004
MmeI TCCRAC 6 cut(s) 232, 431, 616, 994, 1421, 3533
Mox20I TGGCCA 2 cut(s) 884, 3185
Mph1103I ATGCAT 4 cut(s) 369, 805, 865, 4188
MroI TCCGGA 2 cut(s) 1242, 1273
MroXI GAANNNNTTC 1 cut(s) 2736
MscI TGGCCA 2 cut(s) 884, 3185
MslI CAYNNNNRTG 6 cut(s) 107, 1050, 1853, 2403, 2420, 3861
Msp20I TGGCCA 2 cut(s) 884, 3185
MspA1I CMGCKG 2 cut(s) 52, 188
MspI CCGG 7 cut(s) 59, 83, 134, 377, 1243, 1274, 1963
MspR9I CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
MunI CAATTG 1 cut(s) 1677
Mva1269I GAATGC 4 cut(s) 1843, 1974, 3101, 3832
MvaI CCWGG 4 cut(s) 536, 1025, 1684, 3903
MvnI CGCG 1 cut(s) 272
NciI CCSGG 1 cut(s) 1963
NdeI CATATG 2 cut(s) 805, 973
NheI GCTAGC 1 cut(s) 818
NmeAIII GCCGAG 1 cut(s) 1795
NmuCI GTSAC 6 cut(s) 370, 1477, 1726, 2454, 2612, 2668
NotI GCGGCCGC 1 cut(s) 264
NsiI ATGCAT 4 cut(s) 369, 805, 865, 4188
NspI RCATGY 3 cut(s) 371, 1583, 3875
NspV TTCGAA 1 cut(s) 2104
PagI TCATGA 1 cut(s) 1033
PaqCI CACCTGC 1 cut(s) 224
PciI ACATGT 1 cut(s) 3871
PciSI GCTCTTC 2 cut(s) 1650, 3243
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PctI GAATGC 4 cut(s) 1843, 1974, 3101, 3832
PdmI GAANNNNTTC 1 cut(s) 2736
PflMI CCANNNNNTGG 1 cut(s) 3647
PleI GAGTC 5 cut(s) 49, 94, 2129, 2160, 4003
PpsI GAGTC 5 cut(s) 49, 94, 2129, 2160, 4003
Ppu21I YACGTR 1 cut(s) 43
PpuMI RGGWCCY 1 cut(s) 4137
PscI ACATGT 1 cut(s) 3871
PshAI GACNNNNGTC 1 cut(s) 497
PshBI ATTAAT 1 cut(s) 4031
PsiI TTATAA 5 cut(s) 1061, 1392, 1832, 2448, 3687
Psp124BI GAGCTC 1 cut(s) 3132
Psp1406I AACGTT 1 cut(s) 2362
Psp5II RGGWCCY 1 cut(s) 4137
Psp6I CCWGG 4 cut(s) 534, 1023, 1682, 3901
PspGI CCWGG 4 cut(s) 534, 1023, 1682, 3901
PspPI GGNCC 5 cut(s) 30, 731, 2311, 4018, 4137
PspPPI RGGWCCY 1 cut(s) 4137
PstNI CAGNNNCTG 2 cut(s) 52, 3647
PsuI RGATCY 7 cut(s) 400, 1349, 1486, 1777, 3153, 3283, 3673
RsaI GTAC 9 cut(s) 45, 101, 296, 386, 1224, 1311, 1888, 2440, 3251
RsaNI GTAC 9 cut(s) 44, 100, 295, 385, 1223, 1310, 1887, 2439, 3250
RseI CAYNNNNRTG 6 cut(s) 107, 1050, 1853, 2403, 2420, 3861
SacI GAGCTC 1 cut(s) 3132
SapI GCTCTTC 2 cut(s) 1650, 3243
Sau96I GGNCC 5 cut(s) 30, 731, 2311, 4018, 4137
SchI GAGTC 5 cut(s) 49, 95, 2129, 2161, 4004
ScrFI CCNGG 5 cut(s) 536, 1025, 1684, 1963, 3903
SduI GDGCHC 5 cut(s) 678, 3132, 3348, 3608, 3735
SfcI CTRYAG 4 cut(s) 1995, 2593, 3133, 3570
SfuI TTCGAA 1 cut(s) 2104
SinI GGWCC 4 cut(s) 30, 731, 2311, 4137
SmiMI CAYNNNNRTG 6 cut(s) 107, 1050, 1853, 2403, 2420, 3861
SmlI CTYRAG 5 cut(s) 1604, 2617, 3065, 3299, 3479
SmoI CTYRAG 5 cut(s) 1604, 2617, 3065, 3299, 3479
SpeI ACTAGT 1 cut(s) 1873
SspI AATATT 5 cut(s) 793, 1083, 1234, 1522, 2880
SspMI CTAG 8 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3719, 3789
SstI GAGCTC 1 cut(s) 3132
StyD4I CCNGG 5 cut(s) 534, 1023, 1682, 1961, 3901
StyI CCWWGG 3 cut(s) 1093, 2193, 3712
TaiI ACGT 6 cut(s) 45, 125, 1798, 2365, 3382, 4092
TatI WGTACW 3 cut(s) 294, 1222, 2438
TseFI GTSAC 6 cut(s) 370, 1477, 1726, 2454, 2612, 2668
Tsp45I GTSAC 6 cut(s) 370, 1477, 1726, 2454, 2612, 2668
TspGWI ACGGA 2 cut(s) 3253, 4166
Van91I CCANNNNNTGG 1 cut(s) 3647
VpaK11BI GGWCC 4 cut(s) 30, 731, 2311, 4137
VspI ATTAAT 1 cut(s) 4031
XapI RAATTY 1 cut(s) 1444
XbaI TCTAGA 1 cut(s) 1774
XceI RCATGY 3 cut(s) 371, 1583, 3875
XcmI CCANNNNNNNNNTGG 1 cut(s) 3961
XmiI GTMKAC 1 cut(s) 2631
XmnI GAANNNNTTC 1 cut(s) 2736
XspI CTAG 8 cut(s) 819, 956, 1214, 1475, 1775, 1874, 3719, 3789
Zsp2I ATGCAT 4 cut(s) 369, 805, 865, 4188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.