MD07G1224400.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014271
Physical Location & Seq
Reverse (-)
30097739 .. 30098806
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1224400.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAAGAAGACCAAGACCGCGATTCGGCAGCTGAAGCAGCACCAGATCATCACCTTCCCCAAGAAAACAGCAACAAAACCACCGAGGACAACTCGACCACCGCATCCACCGCCACAACAGAAACAACGGACAACAACAACAGAAAGTGCAAGGGCAAAGGAGGCCCCGAAAACAACAAGTTCAGGTACCGCGGCGTGCGTCAGCGGAGCTGGGGCAAATGGGTAGCTGAGATCCGCGAGCCGCGAAAGCGCACTCGCAAGTGGCTGGGGACCTTCTCCACTGCCGAGGATGCTGCCCGCGCCTACGACCGGGCCGCCATCATCCTCTACGGTTCCAGGGCACAGCTCAACCTCCAGCCCTCCGGTTCCTCGTCCCAGAACTCCACGCGCGGCTCCTCTTCTTCCTCCTCATCGTCGAGCTCGTCCACCCAAACCCTAAGACCCCTTCTCCCTCGCCCCTCCGGCTTCGGCCTTACTCTGCCCCACCTCTCCTCCGCTCACCCGGTCCCACTCATGGCTTCGGGGTTCGTTCCTTACGGTGTTGAAGTAGGGTTAGGAGTCTACCCTAACGTTGCTACTGCTGCCGCCGTCGCCGGTGGTGGTGCAACTTCGTCCGTGCGTTTGAATCAGCATCCTCATCATAATAATATGTTAGATCAAGATCATCCTCAAGACAACATTAATCCATTGCACCAACAACTACAGCAACAGCAGCAGGTTGTGGTGCAACACTTTCATCATCAGTACCCCATCTCGTTATCTGACAGATCATCAACCTCGTATCAATATCCAAACTCTAGTCACGATCAATACCAACTACAACTACACCATCAGAATAACAATAATCTGGAGTGTTGTTCGCACGACGATGTCATGAATTCGCTTGTGGGATCGGGGTTGTCTACGGAGCCTATGGCGGTTGCACCGGGGTGTTCTGACATTTCGATGGGGGGAGTGGGGCCCATTTCGCCGTCGATGTGGCCGCTGACAAGCGAGGAAGAGTGCGTGCCGAGCCTTTGGGATTACGGCGATCCTTTCTTCTTGGATTTGAAGGGATTGGATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000272 GO:0001067 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009755 GO:0009756 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009987 GO:0010033 GO:0010035 GO:0010119 GO:0010154 GO:0010182 GO:0010353 GO:0010449 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010896 GO:0016052 GO:0019216 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031930 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032880 GO:0033993 GO:0034284 GO:0034285 GO:0035266 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044042 GO:0044212 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048316 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0050994 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080090 GO:0090207 GO:0090696 GO:0097159 GO:0097305 GO:0097306 GO:0099402 GO:0140110 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

356

Amino Acids

38.76

Weight (kDa)

5.94

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 62 - 111 4.2e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40220
fragaria_vesca FvH4_7g24760
malus_domestica MD01G1155400.v1.1 MD07G1224400.v1.1
prunus_persica Prupe.2G253000_v2.0.a1
pyrus_communis pycom01g17270 pycom13g15020
rosa_chinensis RchiOBHm_Chr1g0370631
rosa_laevigata RLG00000027023
rosa_multiflora Rmu_sc0002021.1_g000011
rosa_roxburghii Rroxscaffold_4G00286390
rosa_rugosa Rorug01G0358000
rosa_samantha Rh1AG367500 Rh1BG330800 Rh1CG345000 Rh1DG362300
rosa_wichuraiana Rw1G032340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 706
Acc65I GGTACC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 186
AccBSI CCGCTC 1 cut(s) 497
AccI GTMKAC 2 cut(s) 561, 902
AccII CGCG 7 cut(s) 21, 192, 237, 244, 300, 388, 390
AclI AACGTT 1 cut(s) 570
AclWI GGATC 3 cut(s) 226, 898, 1025
AcoI YGGCCR 1 cut(s) 980
AcsI RAATTY 1 cut(s) 877
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 2 cut(s) 188, 746
AfiI CCNNNNNNNGG 3 cut(s) 25, 309, 514
AgsI TTSAA 3 cut(s) 545, 625, 1051
AjnI CCWGG 1 cut(s) 335
AleI CACNNNNGTG 1 cut(s) 928
AluBI AGCT 5 cut(s) 32, 210, 227, 346, 420
AluI AGCT 5 cut(s) 32, 210, 227, 346, 420
Alw21I GWGCWC 1 cut(s) 422
AlwI GGATC 3 cut(s) 226, 898, 1025
AoxI GGCC 5 cut(s) 163, 312, 469, 959, 980
ApaI GGGCCC 1 cut(s) 963
ApeKI GCWGC 5 cut(s) 29, 38, 293, 581, 712
ApoI RAATTY 1 cut(s) 877
AseI ATTAAT 1 cut(s) 681
Asp718I GGTACC 1 cut(s) 186
AspLEI GCGC 3 cut(s) 252, 302, 390
AspS9I GGNCC 6 cut(s) 164, 270, 312, 505, 959, 960
AsuC2I CCSGG 3 cut(s) 311, 503, 927
AsuHPI GGTGA 2 cut(s) 44, 491
AvaII GGWCC 2 cut(s) 270, 505
BaeGI GKGCMC 2 cut(s) 343, 963
BanI GGYRCC 1 cut(s) 186
BanII GRGCYC 2 cut(s) 422, 963
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 422
BbvI GCAGC 5 cut(s) 41, 50, 280, 568, 724
BccI CCATC 4 cut(s) 326, 758, 837, 940
BceAI ACGGC 3 cut(s) 572, 955, 1042
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 1 cut(s) 337
BcnI CCSGG 3 cut(s) 311, 503, 927
BfaI CTAG 1 cut(s) 798
BfmI CTRYAG 1 cut(s) 701
BfuAI ACCTGC 1 cut(s) 706
BglI GCCNNNNNGGC 1 cut(s) 462
Bme1390I CCNGG 4 cut(s) 311, 337, 503, 927
Bme18I GGWCC 2 cut(s) 270, 505
BmgT120I GGNCC 6 cut(s) 164, 270, 312, 505, 959, 960
BmrFI CCNGG 4 cut(s) 311, 337, 503, 927
BmsI GCATC 3 cut(s) 113, 280, 640
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 2 cut(s) 338, 869
BpuEI CTTGAG 1 cut(s) 654
BpuMI CCSGG 3 cut(s) 311, 503, 927
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 5 cut(s) 84, 190, 285, 336, 926
BsaWI WCCGGW 1 cut(s) 362
BsaXI ACNNNNNCTCC 4 cut(s) 432, 462, 476, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 309, 514
Bse118I RCCGGY 1 cut(s) 593
Bse3DI GCAATG 1 cut(s) 686
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 5 cut(s) 84, 190, 285, 336, 926
BseGI GGATG 5 cut(s) 104, 295, 321, 631, 664
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 3 cut(s) 25, 309, 514
BseMI GCAATG 1 cut(s) 686
BseMII CTCAG 1 cut(s) 219
BseRI GAGGAG 3 cut(s) 385, 397, 481
BseSI GKGCMC 2 cut(s) 343, 963
BseXI GCAGC 5 cut(s) 41, 50, 280, 568, 724
BseYI CCCAGC 2 cut(s) 210, 265
Bsh1236I CGCG 7 cut(s) 21, 192, 237, 244, 300, 388, 390
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 5 cut(s) 165, 314, 471, 961, 982
BshNI GGYRCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 422
BsiSI CCGG 6 cut(s) 310, 363, 462, 503, 594, 926
BslFI GGGAC 3 cut(s) 283, 358, 491
BslI CCNNNNNNNGG 3 cut(s) 25, 309, 514
BsmFI GGGAC 3 cut(s) 283, 358, 491
BsnI GGCC 5 cut(s) 165, 314, 471, 961, 982
Bsp120I GGGCCC 1 cut(s) 959
Bsp1286I GDGCHC 3 cut(s) 343, 422, 963
Bsp143I GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
BspANI GGCC 5 cut(s) 165, 314, 471, 961, 982
BspCNI CTCAG 1 cut(s) 220
BspFNI CGCG 7 cut(s) 21, 192, 237, 244, 300, 388, 390
BspHI TCATGA 1 cut(s) 873
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 3 cut(s) 226, 898, 1025
BspT107I GGYRCC 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 593
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 5 cut(s) 84, 190, 285, 336, 926
BssMI GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 2 cut(s) 332, 539
Bst6I CTCTTC 2 cut(s) 403, 993
BstC8I GCNNGC 4 cut(s) 197, 239, 298, 1007
BstDEI CTNAG 2 cut(s) 228, 437
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 5 cut(s) 104, 295, 321, 631, 664
BstFNI CGCG 7 cut(s) 21, 192, 237, 244, 300, 388, 390
BstHHI GCGC 3 cut(s) 252, 302, 390
BstKTI GATC 8 cut(s) 49, 234, 658, 664, 770, 808, 893, 1033
BstMBI GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
BstMCI CGRYCG 1 cut(s) 310
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 4 cut(s) 309, 335, 501, 925
BstSFI CTRYAG 1 cut(s) 701
BstSLI GKGCMC 2 cut(s) 343, 963
BstUI CGCG 7 cut(s) 21, 192, 237, 244, 300, 388, 390
BstV1I GCAGC 5 cut(s) 41, 50, 280, 568, 724
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 5 cut(s) 165, 314, 471, 961, 982
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 5 cut(s) 104, 295, 321, 631, 664
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 279
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 4 cut(s) 197, 239, 298, 1007
CciI TCATGA 1 cut(s) 873
CfoI GCGC 3 cut(s) 252, 302, 390
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 6 cut(s) 164, 270, 312, 505, 959, 960
Cfr42I CCGCGG 1 cut(s) 193
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 187, 745
CviAII CATG 2 cut(s) 514, 874
CviQI GTAC 2 cut(s) 187, 745
DdeI CTNAG 2 cut(s) 228, 437
DpnI GATC 8 cut(s) 48, 233, 657, 663, 769, 807, 892, 1032
DpnII GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
EaeI YGGCCR 1 cut(s) 980
Eam1104I CTCTTC 2 cut(s) 403, 993
EarI CTCTTC 2 cut(s) 403, 993
Ecl136II GAGCTC 1 cut(s) 420
Eco24I GRGCYC 2 cut(s) 422, 963
Eco47I GGWCC 2 cut(s) 270, 505
Eco53kI GAGCTC 1 cut(s) 420
Eco57I CTGAAG 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 420
EcoO109I RGGNCCY 3 cut(s) 164, 270, 959
EcoRI GAATTC 1 cut(s) 877
EcoRII CCWGG 1 cut(s) 335
EcoT38I GRGCYC 2 cut(s) 422, 963
FaeI CATG 2 cut(s) 517, 877
FaiI YATR 5 cut(s) 515, 642, 650, 875, 914
FaqI GGGAC 3 cut(s) 283, 358, 491
FatI CATG 2 cut(s) 513, 873
FauI CCCGC 1 cut(s) 305
FblI GTMKAC 2 cut(s) 561, 902
FokI GGATG 5 cut(s) 91, 302, 308, 618, 651
FriOI GRGCYC 2 cut(s) 422, 963
FspBI CTAG 1 cut(s) 798
GlaI GCGC 3 cut(s) 251, 301, 389
GsaI CCCAGC 2 cut(s) 214, 269
GsuI CTGGAG 2 cut(s) 338, 869
HaeIII GGCC 5 cut(s) 165, 314, 471, 961, 982
HapII CCGG 6 cut(s) 310, 363, 462, 503, 594, 926
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 252, 302, 390
Hin1II CATG 2 cut(s) 517, 877
Hin6I GCGC 3 cut(s) 250, 300, 388
HinP1I GCGC 3 cut(s) 250, 300, 388
HinfI GANTC 3 cut(s) 23, 558, 625
HpaII CCGG 6 cut(s) 310, 363, 462, 503, 594, 926
HphI GGTGA 2 cut(s) 44, 491
Hpy166II GTNNAC 3 cut(s) 426, 562, 903
Hpy188I TCNGA 3 cut(s) 763, 834, 937
Hpy188III TCNNGA 5 cut(s) 659, 671, 803, 848, 874
Hpy8I GTNNAC 3 cut(s) 426, 562, 903
Hpy99I CGWCG 4 cut(s) 418, 593, 869, 976
HpyAV CCTTC 4 cut(s) 65, 283, 455, 1045
HpyCH4III ACNGT 2 cut(s) 332, 539
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 5 cut(s) 150, 605, 691, 727, 923
HpyF3I CTNAG 2 cut(s) 228, 437
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 2 cut(s) 517, 877
HspAI GCGC 3 cut(s) 250, 300, 388
KpnI GGTACC 1 cut(s) 190
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
LmnI GCTCC 3 cut(s) 207, 398, 907
Lsp1109I GCAGC 5 cut(s) 41, 50, 280, 568, 724
LweI GCATC 3 cut(s) 113, 280, 640
MaeI CTAG 1 cut(s) 798
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 1 cut(s) 800
MalI GATC 8 cut(s) 48, 233, 657, 663, 769, 807, 892, 1032
MbiI CCGCTC 1 cut(s) 497
MboI GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
MboII GAAGA 6 cut(s) 17, 20, 390, 393, 1010, 1030
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 3 cut(s) 343, 422, 963
MluCI AATT 1 cut(s) 877
MlyI GAGTC 1 cut(s) 567
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 3 cut(s) 867, 928, 944
MspA1I CMGCKG 4 cut(s) 32, 192, 205, 985
MspI CCGG 6 cut(s) 310, 363, 462, 503, 594, 926
MspR9I CCNGG 4 cut(s) 311, 337, 503, 927
MvaI CCWGG 1 cut(s) 337
MvnI CGCG 7 cut(s) 21, 192, 237, 244, 300, 388, 390
NciI CCSGG 3 cut(s) 311, 503, 927
NdeII GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
NlaIII CATG 2 cut(s) 517, 877
NmeAIII GCCGAG 2 cut(s) 310, 1035
NmuCI GTSAC 1 cut(s) 800
OliI CACNNNNGTG 1 cut(s) 928
PagI TCATGA 1 cut(s) 873
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PfeI GAWTC 2 cut(s) 23, 625
PflFI GACNNNGTC 1 cut(s) 869
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 270
PshBI ATTAAT 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 422
Psp1406I AACGTT 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 335
PspFI CCCAGC 2 cut(s) 210, 265
PspGI CCWGG 1 cut(s) 335
PspOMI GGGCCC 1 cut(s) 959
PspPI GGNCC 6 cut(s) 164, 270, 312, 505, 959, 960
PspPPI RGGWCCY 1 cut(s) 270
PsuI RGATCY 1 cut(s) 231
PsyI GACNNNGTC 1 cut(s) 869
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 2 cut(s) 188, 746
RsaNI GTAC 2 cut(s) 187, 745
RseI CAYNNNNRTG 3 cut(s) 867, 928, 944
SacI GAGCTC 1 cut(s) 422
SacII CCGCGG 1 cut(s) 193
SaqAI TTAA 1 cut(s) 681
Sau3AI GATC 8 cut(s) 46, 231, 655, 661, 767, 805, 890, 1030
Sau96I GGNCC 6 cut(s) 164, 270, 312, 505, 959, 960
SchI GAGTC 1 cut(s) 567
ScrFI CCNGG 4 cut(s) 311, 337, 503, 927
SduI GDGCHC 3 cut(s) 343, 422, 963
SfaNI GCATC 3 cut(s) 113, 280, 640
SfcI CTRYAG 1 cut(s) 701
Sfr303I CCGCGG 1 cut(s) 193
SgrAI CRCCGGYG 1 cut(s) 593
SgrBI CCGCGG 1 cut(s) 193
SinI GGWCC 2 cut(s) 270, 505
SmiMI CAYNNNNRTG 3 cut(s) 867, 928, 944
SmlI CTYRAG 1 cut(s) 669
SmoI CTYRAG 1 cut(s) 669
Sse9I AATT 1 cut(s) 877
SspMI CTAG 1 cut(s) 798
SstI GAGCTC 1 cut(s) 422
StyD4I CCNGG 4 cut(s) 309, 335, 501, 925
TaaI ACNGT 2 cut(s) 332, 539
TaiI ACGT 1 cut(s) 573
TaqI TCGA 4 cut(s) 95, 416, 944, 974
TasI AATT 1 cut(s) 877
TauI GCSGC 6 cut(s) 195, 244, 317, 393, 587, 985
TfiI GAWTC 2 cut(s) 23, 625
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 5 cut(s) 29, 38, 293, 581, 712
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 2 cut(s) 725, 890
TspGWI ACGGA 3 cut(s) 143, 604, 920
TspRI CASTG 1 cut(s) 286
Tth111I GACNNNGTC 1 cut(s) 869
VpaK11BI GGWCC 2 cut(s) 270, 505
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 1 cut(s) 877
XmiI GTMKAC 2 cut(s) 561, 902
XspI CTAG 1 cut(s) 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.