MD01G1155400.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014271
Physical Location & Seq
Reverse (-)
26331160 .. 26332257
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1155400.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGAAGAAGACCAACACCGCGATTCGGCAGCTGAAGCAGCACCAGATCATCACCTTCCCCAAGACAACAGCAACAGCAACAGCAACAAAACCACTGAGGACAACTCTACCACCGCTTCCACCGCCACAACAGAAATAACCAACAACAACAACAGAAAGTGCAAGGGCAAAGGAGGCCCTGACAACAACAAGTTCAGGTACCGCGGCGTGCGCCAGCGCAGCTGGGGCAAATGGGTGGCTGAGATCCGTGAGCCGCGTAAGCGGACGCGCAAGTGGCTCGGGACCTTCGCCACTGCCGAGGACGCTGCCCGCGCCTACGACCGGGCCGCCATCATCCTTTACGGTTCCAGAGCCCAGCTTAACCTCCAGCCCTCCGGTTCCTCGTCCCAGAACTCCACGCGGGGCTCCTCTTCTTCCGCTTCCTCCTCCACCTCCACCTCCTCCACCCAAACCCTAAGACCACTTCTCCCCCGCCCCTCGGGCTTCGGCCTTACTCTGCCCTACCCCTCCTCTGCCCACCCGGTCCCGCTCATGGCTTCGGGGTTCGTTCCATACGGTGTTGACTTAGGGTTAGGAGTCTACCATAACGTTGCTGCTGCTGCGGCCGTCGCCGGTTGTGCTCTAACTTCGTCCGTACGTATGAATCAGCATCCTCATCACATGTTAGATCAAGATCATGAGTATCAAAACAACATTAATCCTTTGCACCAACAACAACAGCAACATCAGCAGCAACAAATTGTGGTGCAACAATTTCATCATCAGTACCCCATCGCATTATCCGGCGGCAGCGGTTGTGACACGTCAACCTCGTATCTACATCCAAACCCTAGTCACGATCAATACCAACGACAAGTACCCCACCAGAATAACAATAATCAGGAGTGCTGTTCGTACGAGGATGTGAATTCGCTTGTGGGATCGGGGTTGTCTACGCAGCCTATGGAGGTTGCACCGGGGTGTTCGGATATTCCTGTGGAGGCAGTGGGACCCATGTCGCCGTTGATGTGGCCGCTGACGAGCGAGGAAGAGTGTGTGCCGGGCCTTTGGGACTACGGCGATCCTTTCTTCTTGGATTTTAAGGGATTGGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000272 GO:0001067 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009755 GO:0009756 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009987 GO:0010033 GO:0010035 GO:0010119 GO:0010154 GO:0010182 GO:0010353 GO:0010449 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010896 GO:0016052 GO:0019216 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031930 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032880 GO:0033993 GO:0034284 GO:0034285 GO:0035266 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044042 GO:0044212 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048316 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0050994 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080090 GO:0090207 GO:0090696 GO:0097159 GO:0097305 GO:0097306 GO:0099402 GO:0140110 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

366

Amino Acids

39.96

Weight (kDa)

6.03

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 66 - 115 3.4e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40220
fragaria_vesca FvH4_7g24760
malus_domestica MD01G1155400.v1.1 MD07G1224400.v1.1
prunus_persica Prupe.2G253000_v2.0.a1
pyrus_communis pycom01g17270 pycom13g15020
rosa_chinensis RchiOBHm_Chr1g0370631
rosa_laevigata RLG00000027023
rosa_multiflora Rmu_sc0002021.1_g000011
rosa_roxburghii Rroxscaffold_4G00286390
rosa_rugosa Rorug01G0358000
rosa_samantha Rh1AG367500 Rh1BG330800 Rh1CG345000 Rh1DG362300
rosa_wichuraiana Rw1G032340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 198
AccB1I GGYRCC 1 cut(s) 198
AccBSI CCGCTC 1 cut(s) 529
AccI GTMKAC 2 cut(s) 579, 932
AccII CGCG 6 cut(s) 21, 204, 256, 268, 312, 400
AclI AACGTT 1 cut(s) 588
AclWI GGATC 3 cut(s) 238, 928, 1055
AcoI YGGCCR 2 cut(s) 603, 1010
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 5 cut(s) 200, 636, 767, 858, 896
AfiI CCNNNNNNNGG 4 cut(s) 25, 321, 478, 532
AflIII ACRYGT 2 cut(s) 660, 801
AjiI CACGTC 1 cut(s) 804
AleI CACNNNNGTG 1 cut(s) 958
AluBI AGCT 3 cut(s) 32, 222, 358
AluI AGCT 3 cut(s) 32, 222, 358
Alw21I GWGCWC 1 cut(s) 622
AlwI GGATC 3 cut(s) 238, 928, 1055
Ama87I CYCGRG 2 cut(s) 278, 478
AoxI GGCC 6 cut(s) 175, 324, 487, 603, 1010, 1042
ApoI RAATTY 1 cut(s) 907
AseI ATTAAT 1 cut(s) 696
Asp718I GGTACC 1 cut(s) 198
AspLEI GCGC 4 cut(s) 213, 219, 270, 314
AspS9I GGNCC 6 cut(s) 176, 282, 324, 523, 989, 1042
AsuC2I CCSGG 4 cut(s) 323, 521, 957, 1041
AsuHPI GGTGA 1 cut(s) 44
AvaI CYCGRG 2 cut(s) 278, 478
AvaII GGWCC 3 cut(s) 282, 523, 989
BanI GGYRCC 1 cut(s) 198
BanII GRGCYC 2 cut(s) 355, 407
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 622
BccI CCATC 2 cut(s) 338, 779
BceAI ACGGC 3 cut(s) 590, 985, 1072
BcgI CGANNNNNNTGC 4 cut(s) 259, 287, 293, 321
BcnI CCSGG 4 cut(s) 323, 521, 957, 1041
BfaI CTAG 1 cut(s) 831
BglI GCCNNNNNGGC 1 cut(s) 480
Bme1390I CCNGG 4 cut(s) 323, 521, 957, 1041
Bme18I GGWCC 3 cut(s) 282, 523, 989
BmeT110I CYCGRG 2 cut(s) 278, 478
BmgBI CACGTC 1 cut(s) 804
BmgT120I GGNCC 6 cut(s) 176, 282, 324, 523, 989, 1042
BmiI GGNNCC 8 cut(s) 200, 283, 346, 379, 406, 525, 990, 991
BmrFI CCNGG 4 cut(s) 323, 521, 957, 1041
BmsI GCATC 1 cut(s) 658
BoxI GACNNNNGTC 1 cut(s) 994
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 89, 121
BpmI CTGGAG 1 cut(s) 350
BpuMI CCSGG 4 cut(s) 323, 521, 957, 1041
BsaAI YACGTR 1 cut(s) 638
BsaBI GATNNNNATC 1 cut(s) 672
BsaJI CCNNGG 4 cut(s) 202, 297, 477, 956
BsaWI WCCGGW 1 cut(s) 374
BsaXI ACNNNNNCTCC 2 cut(s) 450, 480
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 321, 478, 532
Bse118I RCCGGY 1 cut(s) 611
Bse8I GATNNNNATC 1 cut(s) 672
BseDI CCNNGG 4 cut(s) 202, 297, 477, 956
BseGI GGATG 4 cut(s) 333, 649, 820, 907
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 4 cut(s) 25, 321, 478, 532
BseMII CTCAG 2 cut(s) 87, 231
BseRI GAGGAG 4 cut(s) 397, 415, 430, 499
BseX3I CGGCCG 1 cut(s) 603
BseYI CCCAGC 2 cut(s) 222, 354
Bsh1236I CGCG 6 cut(s) 21, 204, 256, 268, 312, 400
Bsh1285I CGRYCG 2 cut(s) 322, 606
BshFI GGCC 6 cut(s) 177, 326, 489, 605, 1012, 1044
BshNI GGYRCC 1 cut(s) 198
BsiEI CGRYCG 2 cut(s) 322, 606
BsiHKAI GWGCWC 1 cut(s) 622
BsiHKCI CYCGRG 2 cut(s) 278, 478
BsiSI CCGG 7 cut(s) 322, 375, 521, 612, 783, 956, 1040
BsiWI CGTACG 2 cut(s) 634, 894
BslFI GGGAC 5 cut(s) 295, 370, 509, 1002, 1064
BslI CCNNNNNNNGG 4 cut(s) 25, 321, 478, 532
BsmFI GGGAC 5 cut(s) 295, 370, 509, 1002, 1064
BsnI GGCC 6 cut(s) 177, 326, 489, 605, 1012, 1044
BsoBI CYCGRG 2 cut(s) 278, 478
Bsp1286I GDGCHC 3 cut(s) 355, 407, 622
Bsp143I GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
BspANI GGCC 6 cut(s) 177, 326, 489, 605, 1012, 1044
BspCNI CTCAG 2 cut(s) 88, 232
BspFNI CGCG 6 cut(s) 21, 204, 256, 268, 312, 400
BspHI TCATGA 2 cut(s) 676, 1094
BspLI GGNNCC 8 cut(s) 200, 283, 346, 379, 406, 525, 990, 991
BspPI GGATC 3 cut(s) 238, 928, 1055
BspT107I GGYRCC 1 cut(s) 198
BsrBI CCGCTC 1 cut(s) 529
BsrFI RCCGGY 1 cut(s) 611
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 4 cut(s) 202, 297, 477, 956
BssMI GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
Bst4CI ACNGT 2 cut(s) 344, 557
Bst6I CTCTTC 2 cut(s) 415, 1023
BstBAI YACGTR 1 cut(s) 638
BstC8I GCNNGC 3 cut(s) 209, 215, 310
BstDEI CTNAG 4 cut(s) 96, 240, 455, 565
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 4 cut(s) 333, 649, 820, 907
BstFNI CGCG 6 cut(s) 21, 204, 256, 268, 312, 400
BstHHI GCGC 4 cut(s) 213, 219, 270, 314
BstKTI GATC 7 cut(s) 49, 246, 670, 676, 841, 923, 1063
BstMBI GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
BstMCI CGRYCG 2 cut(s) 322, 606
BstNSI RCATGY 1 cut(s) 664
BstPAI GACNNNNGTC 1 cut(s) 994
BstSCI CCNGG 4 cut(s) 321, 519, 955, 1039
BstSNI TACGTA 1 cut(s) 638
BstUI CGCG 6 cut(s) 21, 204, 256, 268, 312, 400
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 243
BstYI RGATCY 1 cut(s) 243
BstZI CGGCCG 1 cut(s) 603
BsuRI GGCC 6 cut(s) 177, 326, 489, 605, 1012, 1044
BtgI CCRYGG 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 757
BtrI CACGTC 1 cut(s) 804
BtsCI GGATG 4 cut(s) 333, 649, 820, 907
BtsI GCAGTG 2 cut(s) 291, 990
BtsIMutI CAGTG 3 cut(s) 93, 291, 990
Cac8I GCNNGC 3 cut(s) 209, 215, 310
CciI TCATGA 2 cut(s) 676, 1094
CfoI GCGC 4 cut(s) 213, 219, 270, 314
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 6 cut(s) 176, 282, 324, 523, 989, 1042
Cfr42I CCGCGG 1 cut(s) 205
CseI GACGC 2 cut(s) 274, 311
Csp6I GTAC 5 cut(s) 199, 635, 766, 857, 895
CviAII CATG 5 cut(s) 532, 661, 677, 994, 1095
CviQI GTAC 5 cut(s) 199, 635, 766, 857, 895
DdeI CTNAG 4 cut(s) 96, 240, 455, 565
DpnI GATC 7 cut(s) 48, 245, 669, 675, 840, 922, 1062
DpnII GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
EaeI YGGCCR 2 cut(s) 603, 1010
EagI CGGCCG 1 cut(s) 603
Eam1104I CTCTTC 2 cut(s) 415, 1023
EarI CTCTTC 2 cut(s) 415, 1023
EclXI CGGCCG 1 cut(s) 603
Eco105I TACGTA 1 cut(s) 638
Eco24I GRGCYC 2 cut(s) 355, 407
Eco47I GGWCC 3 cut(s) 282, 523, 989
Eco52I CGGCCG 1 cut(s) 603
Eco57I CTGAAG 1 cut(s) 54
Eco88I CYCGRG 2 cut(s) 278, 478
EcoO109I RGGNCCY 3 cut(s) 176, 282, 989
EcoRI GAATTC 1 cut(s) 907
EcoT38I GRGCYC 2 cut(s) 355, 407
FaeI CATG 5 cut(s) 535, 664, 680, 997, 1098
FaiI YATR 9 cut(s) 533, 553, 585, 641, 662, 678, 944, 995, 1096
FaqI GGGAC 5 cut(s) 295, 370, 509, 1002, 1064
FatI CATG 5 cut(s) 531, 660, 676, 993, 1094
FauI CCCGC 4 cut(s) 317, 393, 479, 534
FblI GTMKAC 2 cut(s) 579, 932
FokI GGATG 4 cut(s) 320, 636, 807, 914
FriOI GRGCYC 2 cut(s) 355, 407
FspBI CTAG 1 cut(s) 831
GlaI GCGC 4 cut(s) 212, 218, 269, 313
GsaI CCCAGC 2 cut(s) 226, 358
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 6 cut(s) 177, 326, 489, 605, 1012, 1044
HapII CCGG 7 cut(s) 322, 375, 521, 612, 783, 956, 1040
HgaI GACGC 2 cut(s) 274, 311
HhaI GCGC 4 cut(s) 213, 219, 270, 314
Hin1II CATG 5 cut(s) 535, 664, 680, 997, 1098
Hin6I GCGC 4 cut(s) 211, 217, 268, 312
HinP1I GCGC 4 cut(s) 211, 217, 268, 312
HincII GTYRAC 2 cut(s) 562, 807
HindII GTYRAC 2 cut(s) 562, 807
HinfI GANTC 4 cut(s) 23, 576, 643, 1091
HpaII CCGG 7 cut(s) 322, 375, 521, 612, 783, 956, 1040
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 4 cut(s) 562, 580, 807, 933
Hpy188I TCNGA 1 cut(s) 967
Hpy188III TCNNGA 7 cut(s) 280, 348, 671, 677, 836, 881, 1095
Hpy8I GTNNAC 4 cut(s) 562, 580, 807, 933
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 2 cut(s) 65, 295
HpyCH4III ACNGT 2 cut(s) 344, 557
HpyCH4IV ACGT 3 cut(s) 588, 637, 803
HpyCH4V TGCA 4 cut(s) 162, 706, 748, 953
HpyF3I CTNAG 4 cut(s) 96, 240, 455, 565
HpySE526I ACGT 3 cut(s) 588, 637, 803
Hsp92II CATG 5 cut(s) 535, 664, 680, 997, 1098
HspAI GCGC 4 cut(s) 211, 217, 268, 312
KflI GGGWCCC 1 cut(s) 989
KpnI GGTACC 1 cut(s) 202
KspI CCGCGG 1 cut(s) 205
Kzo9I GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
LmnI GCTCC 1 cut(s) 410
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 831
MaeII ACGT 3 cut(s) 588, 637, 803
MaeIII GTNAC 2 cut(s) 797, 833
MalI GATC 7 cut(s) 48, 245, 669, 675, 840, 922, 1062
MbiI CCGCTC 1 cut(s) 529
MboI GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
MboII GAAGA 6 cut(s) 17, 20, 402, 405, 1040, 1060
MflI RGATCY 1 cut(s) 243
MhlI GDGCHC 3 cut(s) 355, 407, 622
MluCI AATT 3 cut(s) 738, 752, 907
MlyI GAGTC 1 cut(s) 585
MseI TTAA 3 cut(s) 360, 696, 1080
MslI CAYNNNNRTG 1 cut(s) 958
MspA1I CMGCKG 5 cut(s) 32, 204, 222, 792, 1015
MspI CCGG 7 cut(s) 322, 375, 521, 612, 783, 956, 1040
MspR9I CCNGG 4 cut(s) 323, 521, 957, 1041
MvnI CGCG 6 cut(s) 21, 204, 256, 268, 312, 400
NciI CCSGG 4 cut(s) 323, 521, 957, 1041
NdeII GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
NlaIII CATG 5 cut(s) 535, 664, 680, 997, 1098
NlaIV GGNNCC 8 cut(s) 200, 283, 346, 379, 406, 525, 990, 991
NmeAIII GCCGAG 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 797, 833
NspI RCATGY 1 cut(s) 664
OliI CACNNNNGTG 1 cut(s) 958
PagI TCATGA 2 cut(s) 676, 1094
PciI ACATGT 1 cut(s) 660
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 809
PfeI GAWTC 3 cut(s) 23, 643, 1091
Pfl23II CGTACG 2 cut(s) 634, 894
PleI GAGTC 1 cut(s) 584
PpsI GAGTC 1 cut(s) 584
Ppu21I YACGTR 1 cut(s) 638
PpuMI RGGWCCY 2 cut(s) 282, 989
PscI ACATGT 1 cut(s) 660
PshAI GACNNNNGTC 1 cut(s) 994
PshBI ATTAAT 1 cut(s) 696
Psp1406I AACGTT 1 cut(s) 588
Psp5II RGGWCCY 2 cut(s) 282, 989
PspFI CCCAGC 2 cut(s) 222, 354
PspLI CGTACG 2 cut(s) 634, 894
PspN4I GGNNCC 8 cut(s) 200, 283, 346, 379, 406, 525, 990, 991
PspPI GGNCC 6 cut(s) 176, 282, 324, 523, 989, 1042
PspPPI RGGWCCY 2 cut(s) 282, 989
PsuI RGATCY 1 cut(s) 243
PvuII CAGCTG 2 cut(s) 32, 222
RsaI GTAC 5 cut(s) 200, 636, 767, 858, 896
RsaNI GTAC 5 cut(s) 199, 635, 766, 857, 895
RseI CAYNNNNRTG 1 cut(s) 958
SacII CCGCGG 1 cut(s) 205
SaqAI TTAA 3 cut(s) 360, 696, 1080
Sau3AI GATC 7 cut(s) 46, 243, 667, 673, 838, 920, 1060
Sau96I GGNCC 6 cut(s) 176, 282, 324, 523, 989, 1042
SchI GAGTC 1 cut(s) 585
ScrFI CCNGG 4 cut(s) 323, 521, 957, 1041
SduI GDGCHC 3 cut(s) 355, 407, 622
SfaNI GCATC 1 cut(s) 658
Sfr303I CCGCGG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 205
SinI GGWCC 3 cut(s) 282, 523, 989
SmiMI CAYNNNNRTG 1 cut(s) 958
SnaBI TACGTA 1 cut(s) 638
Sse9I AATT 3 cut(s) 738, 752, 907
SspMI CTAG 1 cut(s) 831
StyD4I CCNGG 4 cut(s) 321, 519, 955, 1039
TaaI ACNGT 2 cut(s) 344, 557
TaiI ACGT 3 cut(s) 591, 640, 806
TasI AATT 3 cut(s) 738, 752, 907
TauI GCSGC 6 cut(s) 207, 256, 329, 605, 789, 1015
TfiI GAWTC 3 cut(s) 23, 643, 1091
Tru1I TTAA 3 cut(s) 360, 696, 1080
Tru9I TTAA 3 cut(s) 360, 696, 1080
TscAI CASTG 3 cut(s) 100, 298, 990
TseFI GTSAC 2 cut(s) 797, 833
Tsp45I GTSAC 2 cut(s) 797, 833
TspDTI ATGAA 3 cut(s) 656, 746, 1083
TspGWI ACGGA 2 cut(s) 236, 622
TspRI CASTG 3 cut(s) 100, 298, 990
VpaK11BI GGWCC 3 cut(s) 282, 523, 989
VspI ATTAAT 1 cut(s) 696
XapI RAATTY 1 cut(s) 907
XceI RCATGY 1 cut(s) 664
XmiI GTMKAC 2 cut(s) 579, 932
XspI CTAG 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.