RchiOBHm_Chr1g0370601

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018730
Physical Location & Seq
Reverse (-)
60097071 .. 60097740
670 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59476

Sequence Viewer

Length: 489 bp
ATGTCGACTAAAGATTCTTCTGGACCCTTCCATGGCCACTACACCGTCCTCGACGACAAAGAGTTCCAAGTAAAGGGCCGCACCCTCTTCTTCGTCGTGATCCTCTTCACCGTCGTCCTCCTCGTCACCGTCTTCTTGCTCTACGCGAGATGGGTTTGCCGCTACCGCCCCTCCAACTCACCTTTCTCTTCACACGCGCCGAACGCGCCGCCGCAGAAGCCGCGCGGCCTCGACGCTGCCCTGATTCGAGGCTTGCCGATAATCTTGTACCAGTCCTCCGGCAATTCGGAGGCGGCTGGCTGCGGTGAGTGTTCTATATGTCTCGGAGTTTTCGAAGACGGCGAGAAGGTTAAGGTTCTCCCTCAGTGCCGCCATTGCTACCACTCTGACTGCGTCGATACGTGGCTGAGCGCTCAGTCCAGTTGCCCGCTCTGCCGTACTTCTCTCCGGTCAAACTCCGATGACGCTCTGCAAATTGTCGTACAGTGA

Protein Analysis

162

Amino Acids

17.88

Weight (kDa)

7.56

Isoelectric Point (pI)

53.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 103 - 146 1.7e-14 Ring finger domain
zf-RING_11 PF17123 103 - 132 1.9e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 103 - 145 3.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 104 - 145 7.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 112 - 146 2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018730)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24750
rosa_chinensis RchiOBHm_Chr1g0370601
rosa_laevigata RLG00000027024
rosa_multiflora Rmu_sc0002021.1_g000012
rosa_roxburghii Rroxscaffold_4G00286400
rosa_rugosa Rorug01G0358000
rosa_samantha Rh1BG330700 Rh1DG362200
rosa_wichuraiana Rw1G032330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 430
AccI GTMKAC 1 cut(s) 5
AccII CGCG 5 cut(s) 146, 197, 206, 223, 225
AclWI GGATC 1 cut(s) 94
AcoI YGGCCR 1 cut(s) 34
AfaI GTAC 3 cut(s) 269, 439, 483
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 2 cut(s) 32, 73
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 1 cut(s) 94
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 3 cut(s) 34, 76, 226
ApeKI GCWGC 2 cut(s) 236, 300
AspLEI GCGC 4 cut(s) 199, 208, 225, 413
AspS9I GGNCC 2 cut(s) 23, 76
AsuHPI GGTGA 4 cut(s) 100, 118, 171, 317
AsuII TTCGAA 1 cut(s) 333
AvaII GGWCC 1 cut(s) 23
BalI TGGCCA 1 cut(s) 36
BbsI GAAGAC 2 cut(s) 124, 342
BbvI GCAGC 2 cut(s) 223, 287
BccI CCATC 1 cut(s) 144
BceAI ACGGC 2 cut(s) 355, 420
BcoDI GTCTC 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 414
BlpI GCTNAGC 1 cut(s) 407
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 23, 76
BmiI GGNNCC 1 cut(s) 25
BpiI GAAGAC 2 cut(s) 124, 342
Bpu1102I GCTNAGC 1 cut(s) 407
Bpu14I TTCGAA 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 447
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 260, 290
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 73
Bse1I ACTGG 2 cut(s) 271, 420
Bse3DI GCAATG 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 31
BseLI CCNNNNNNNGG 2 cut(s) 32, 73
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 3 cut(s) 377, 398, 428
BseNI ACTGG 2 cut(s) 271, 420
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 2 cut(s) 223, 287
Bsh1236I CGCG 5 cut(s) 146, 197, 206, 223, 225
BshFI GGCC 3 cut(s) 36, 78, 228
BsiSI CCGG 2 cut(s) 279, 448
BslI CCNNNNNNNGG 2 cut(s) 32, 73
BsmAI GTCTC 1 cut(s) 326
BsnI GGCC 3 cut(s) 36, 78, 228
Bsp119I TTCGAA 1 cut(s) 333
Bsp143I GATC 1 cut(s) 99
Bsp1720I GCTNAGC 1 cut(s) 407
Bsp19I CCATGG 1 cut(s) 31
BspANI GGCC 3 cut(s) 36, 78, 228
BspCNI CTCAG 3 cut(s) 376, 399, 427
BspFNI CGCG 5 cut(s) 146, 197, 206, 223, 225
BspLI GGNNCC 1 cut(s) 25
BspPI GGATC 1 cut(s) 94
BspT104I TTCGAA 1 cut(s) 333
BsrBI CCGCTC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 373
BsrI ACTGG 2 cut(s) 271, 420
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 31
Bst4CI ACNGT 4 cut(s) 46, 112, 130, 486
Bst6I CTCTTC 3 cut(s) 92, 110, 193
BstBAI YACGTR 1 cut(s) 402
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 254, 298, 428
BstDEI CTNAG 3 cut(s) 363, 407, 414
BstDSI CCRYGG 1 cut(s) 31
BstFNI CGCG 5 cut(s) 146, 197, 206, 223, 225
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 4 cut(s) 199, 208, 225, 413
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 7 cut(s) 165, 203, 205, 217, 220, 375, 432
BstUI CGCG 5 cut(s) 146, 197, 206, 223, 225
BstV1I GCAGC 2 cut(s) 223, 287
BstV2I GAAGAC 2 cut(s) 124, 342
BsuRI GGCC 3 cut(s) 36, 78, 228
BtgI CCRYGG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 371
Cac8I GCNNGC 3 cut(s) 254, 298, 428
CfoI GCGC 4 cut(s) 199, 208, 225, 413
Cfr13I GGNCC 2 cut(s) 23, 76
CseI GACGC 3 cut(s) 242, 382, 473
Csp6I GTAC 3 cut(s) 268, 438, 482
CviAII CATG 1 cut(s) 32
CviJI RGCY 8 cut(s) 36, 78, 220, 228, 252, 296, 300, 406
CviKI_1 RGCY 8 cut(s) 36, 78, 220, 228, 252, 296, 300, 406
CviQI GTAC 3 cut(s) 268, 438, 482
DdeI CTNAG 3 cut(s) 363, 407, 414
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 3 cut(s) 92, 110, 193
EarI CTCTTC 3 cut(s) 92, 110, 193
Eco130I CCWWGG 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 23
Eco47III AGCGCT 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 1 cut(s) 35
FaiI YATR 3 cut(s) 33, 317, 319
FatI CATG 1 cut(s) 31
FauI CCCGC 1 cut(s) 435
FblI GTMKAC 1 cut(s) 5
GlaI GCGC 4 cut(s) 198, 207, 224, 412
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 3 cut(s) 36, 78, 228
HapII CCGG 2 cut(s) 279, 448
HgaI GACGC 3 cut(s) 242, 382, 473
HhaI GCGC 4 cut(s) 199, 208, 225, 413
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 4 cut(s) 197, 206, 223, 411
HinP1I GCGC 4 cut(s) 197, 206, 223, 411
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 14, 244
HpaII CCGG 2 cut(s) 279, 448
HphI GGTGA 4 cut(s) 100, 118, 171, 317
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 289, 326, 388, 460
Hpy188III TCNNGA 2 cut(s) 21, 97
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 5 cut(s) 56, 98, 116, 236, 398
HpyAV CCTTC 2 cut(s) 37, 340
HpyCH4III ACNGT 4 cut(s) 46, 112, 130, 486
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 1 cut(s) 472
HpyF10VI GCNNNNNNNGC 7 cut(s) 165, 203, 205, 217, 220, 375, 432
HpyF3I CTNAG 3 cut(s) 363, 407, 414
HpySE526I ACGT 1 cut(s) 401
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 4 cut(s) 197, 206, 223, 411
Kzo9I GATC 1 cut(s) 99
LpnPI CCDG 7 cut(s) 6, 254, 282, 284, 292, 433, 461
Lsp1109I GCAGC 2 cut(s) 223, 287
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 101
MbiI CCGCTC 1 cut(s) 430
MboI GATC 1 cut(s) 99
MboII GAAGA 7 cut(s) 9, 79, 82, 97, 124, 180, 347
MlsI TGGCCA 1 cut(s) 36
MluCI AATT 2 cut(s) 283, 474
MluNI TGGCCA 1 cut(s) 36
MmeI TCCRAC 1 cut(s) 198
Mox20I TGGCCA 1 cut(s) 36
MscI TGGCCA 1 cut(s) 36
MseI TTAA 1 cut(s) 351
Msp20I TGGCCA 1 cut(s) 36
MspI CCGG 2 cut(s) 279, 448
MvnI CGCG 5 cut(s) 146, 197, 206, 223, 225
MwoI GCNNNNNNNGC 7 cut(s) 165, 203, 205, 217, 220, 375, 432
NcoI CCATGG 1 cut(s) 31
NdeII GATC 1 cut(s) 99
NlaIII CATG 1 cut(s) 35
NlaIV GGNNCC 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 124
NspV TTCGAA 1 cut(s) 333
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 330, 339
PfeI GAWTC 2 cut(s) 14, 244
PflFI GACNNNGTC 1 cut(s) 392
Ppu21I YACGTR 1 cut(s) 402
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 2 cut(s) 23, 76
PsyI GACNNNGTC 1 cut(s) 392
RsaI GTAC 3 cut(s) 269, 439, 483
RsaNI GTAC 3 cut(s) 268, 438, 482
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 351
Sau3AI GATC 1 cut(s) 99
Sau96I GGNCC 2 cut(s) 23, 76
SetI ASST 4 cut(s) 184, 351, 357, 404
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 1 cut(s) 23
Sse9I AATT 2 cut(s) 283, 474
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 4 cut(s) 46, 112, 130, 486
TaiI ACGT 1 cut(s) 404
TaqI TCGA 6 cut(s) 5, 51, 231, 247, 333, 396
TasI AATT 2 cut(s) 283, 474
TauI GCSGC 8 cut(s) 81, 162, 211, 214, 223, 228, 296, 372
TfiI GAWTC 2 cut(s) 14, 244
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 371
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 2 cut(s) 236, 300
Tsp45I GTSAC 1 cut(s) 124
TspRI CASTG 1 cut(s) 371
Tth111I GACNNNGTC 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.