FvH4_7g24970

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016395
Physical Location & Seq
Forward (+)
19160594 .. 19163477
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24970.t1

Sequence Viewer

Length: 1113 bp
ATGGATGATCATGAGAAACTGGTCCAAGTCTCCGAGCAAGAAGTCCGAATAGACTTCGCCCTCAACCAGAAATGCCGCGCCAACGTCACCCTCACCTCCCTGATCTCCACGTGTCCCGTCGCCTTCAAAGTCCAAACCTCCTCCCCTCACAAGTTCCTCGTGAAGCCCCCCACCGGCGTCATCCCTCCCTCATCCTCCGCCACCTTCCAGGTCATCCTCAAGCCCCAGCCCCACCTCCCTCCCTCCTTCCCCCGCTCCCCCTCCGACCGCTTCCTCATCAAGGCCGCCCGCCTCCCCCACCCCTCCGCTGACGTTAACGCCTTCTTCAAGGGGCCCCGCCGCACAAGCGATTTGAAACTGAAAGTCGCGTTTGTGGGGCCCGTGCTGCTGAAGCACGCCGTGCGCGGCGGGGATTTGGAGGCGGTGAGGAATATTATCAGGAGGCAGAGGACGGTGCTGGCGGAGATGCGGCCCGCCGAGGCGGAGGAGCTGCTGAGAGTCGCGACGGGGCTGGACGAGCCGGAGAGGATGATTAATTTGCTGCTGGAGGCCGGGTTGAGGATCGAGGCGGCGGGTGTGAGGAGAGATGCGGTGGATGAGCGGCGCGTGGCGGAGGCTTCTGATCGGTTTGCGAGCGTTTTGGGGTTGAGAGAGGCTGGACCGTCGAAAGGGGAAGGAATCAGGTTATCTAAAGATCACGGGCGTCGACGGGTGGGTACTGGCAGTGATAAGCTAGAATTGGGGGAAAAACTGTTGATGGCAGCGCGAATCGGGGATTTGAGAGATGTTGAACTGTTGTTTCTCGAAGGAGCAGATATTAGCTACTGTGATCAATATGGCTTGACAGCTCTTCACGCGGCGGCCATTAAAGGGCACAAGGAGGTTGTTCAGATGCTGGTCGAATTCGGGCTAGACTTGGAATCCAGAGACGGGGAAGGCCATGCGCCGCTGCATCTGGCAGTTGTGGGGGGTAACTTGGAGACGGTTGAGATGTTGATACAGAATGGGGCTAATGTCAATGCTAAGAGCAAGAGTGGGGCTACTCCTTTGTACATGGCCACAGCTATGGGGTATGATGATATCATTGAGTTTCTTATCAGCAGAGGCGGCTGA

Protein Analysis

371

Amino Acids

40.32

Weight (kDa)

7.78

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 21 - 110 2.3e-14 MSP (Major sperm protein) domain
Ank_2 PF12796 251 - 342 1.9e-20 Ankyrin repeats (3 copies)
Ank_4 PF13637 254 - 300 4.5e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 274 - 320 4.3e-07 Ankyrin repeats (many copies)
Ank PF00023 279 - 310 5.5e-06 Ankyrin repeat
Ank_4 PF13637 284 - 333 2.3e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 299 - 350 2.4e-06 Ankyrin repeats (many copies)
Ank PF00023 313 - 343 7.1e-08 Ankyrin repeat
Ank_4 PF13637 317 - 366 3.4e-10 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016395)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24970 FvH4_7g24970
malus_domestica MD01G1157100.v1.1
prunus_persica Prupe.2G254900_v2.0.a1
pyrus_communis pycom01g17390
rosa_chinensis RchiOBHm_Chr1g0370871
rosa_laevigata RLG00000027003
rosa_multiflora Rmu_sc0009474.1_g000017
rosa_roxburghii Rroxscaffold_4G00286170
rosa_rugosa Rorug01G0360000
rosa_samantha Rh1AG369700 Rh1BG333300 Rh1CG346700 Rh1DG364500
rosa_wichuraiana Rw1G032550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 255, 601
AccI GTMKAC 1 cut(s) 706
AccII CGCG 7 cut(s) 78, 368, 405, 503, 606, 766, 857
AclWI GGATC 1 cut(s) 569
AcoI YGGCCR 2 cut(s) 861, 1056
AcsI RAATTY 1 cut(s) 902
AcuI CTGAAG 1 cut(s) 410
AcvI CACGTG 1 cut(s) 111
AcyI GRCGYC 2 cut(s) 177, 703
AdeI CACNNNGTG 1 cut(s) 400
AfaI GTAC 2 cut(s) 718, 1052
AfiI CCNNNNNNNGG 6 cut(s) 173, 280, 558, 668, 870, 930
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 4 cut(s) 127, 328, 355, 791
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 5 cut(s) 490, 733, 822, 848, 1064
AluI AGCT 5 cut(s) 490, 733, 822, 848, 1064
Alw26I GTCTC 3 cut(s) 34, 921, 974
AlwI GGATC 1 cut(s) 569
AlwNI CAGNNNCTG 1 cut(s) 895
AoxI GGCC 8 cut(s) 282, 332, 377, 470, 549, 861, 937, 1056
ApaI GGGCCC 2 cut(s) 336, 381
ApeKI GCWGC 5 cut(s) 385, 490, 541, 761, 949
ApoI RAATTY 1 cut(s) 902
AseI ATTAAT 1 cut(s) 534
AspLEI GCGC 5 cut(s) 80, 405, 606, 766, 946
AspS9I GGNCC 7 cut(s) 22, 332, 333, 377, 378, 471, 659
AsuC2I CCSGG 1 cut(s) 553
AsuHPI GGTGA 3 cut(s) 79, 85, 436
AvaII GGWCC 2 cut(s) 22, 659
BaeGI GKGCMC 3 cut(s) 336, 381, 876
BalI TGGCCA 1 cut(s) 1058
BanII GRGCYC 2 cut(s) 336, 381
BauI CACGAG 1 cut(s) 158
BbrPI CACGTG 1 cut(s) 111
BbvI GCAGC 5 cut(s) 372, 477, 528, 773, 936
BccI CCATC 1 cut(s) 751
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 2 cut(s) 7, 829
BcnI CCSGG 1 cut(s) 553
BcoDI GTCTC 3 cut(s) 34, 921, 974
BfaI CTAG 2 cut(s) 734, 911
Bme1390I CCNGG 2 cut(s) 209, 553
Bme18I GGWCC 2 cut(s) 22, 659
BmgT120I GGNCC 7 cut(s) 22, 332, 333, 377, 378, 471, 659
BmiI GGNNCC 5 cut(s) 333, 334, 335, 378, 379
BmrFI CCNGG 2 cut(s) 209, 553
BmsI GCATC 4 cut(s) 456, 577, 882, 961
BpmI CTGGAG 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 553
BsaAI YACGTR 1 cut(s) 111
BsaHI GRCGYC 2 cut(s) 177, 703
BsaJI CCNNGG 1 cut(s) 477
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 6 cut(s) 173, 280, 558, 668, 870, 930
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 24, 724
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 477
BseGI GGATG 6 cut(s) 10, 180, 191, 213, 534, 601
BseLI CCNNNNNNNGG 6 cut(s) 173, 280, 558, 668, 870, 930
BseMII CTCAG 1 cut(s) 485
BseNI ACTGG 2 cut(s) 24, 724
BseRI GAGGAG 3 cut(s) 130, 500, 595
BseSI GKGCMC 3 cut(s) 336, 381, 876
BseXI GCAGC 5 cut(s) 372, 477, 528, 773, 936
BseYI CCCAGC 1 cut(s) 225
Bsh1236I CGCG 7 cut(s) 78, 368, 405, 503, 606, 766, 857
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 8 cut(s) 284, 334, 379, 472, 551, 863, 939, 1058
BsiEI CGRYCG 1 cut(s) 268
BsiSI CCGG 3 cut(s) 174, 521, 552
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 6 cut(s) 173, 280, 558, 668, 870, 930
BsmAI GTCTC 3 cut(s) 34, 921, 974
BsmBI CGTCTC 2 cut(s) 921, 974
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 8 cut(s) 284, 334, 379, 472, 551, 863, 939, 1058
Bsp120I GGGCCC 2 cut(s) 332, 377
Bsp1286I GDGCHC 3 cut(s) 336, 381, 876
Bsp1407I TGTACA 1 cut(s) 1050
Bsp143I GATC 6 cut(s) 7, 102, 561, 622, 694, 829
Bsp68I TCGCGA 1 cut(s) 503
BspANI GGCC 8 cut(s) 284, 334, 379, 472, 551, 863, 939, 1058
BspCNI CTCAG 1 cut(s) 486
BspFNI CGCG 7 cut(s) 78, 368, 405, 503, 606, 766, 857
BspHI TCATGA 1 cut(s) 10
BspLI GGNNCC 5 cut(s) 333, 334, 335, 378, 379
BspPI GGATC 1 cut(s) 569
BspQI GCTCTTC 1 cut(s) 855
BsrBI CCGCTC 2 cut(s) 255, 601
BsrFI RCCGGY 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 2 cut(s) 24, 724
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 6 cut(s) 7, 102, 561, 622, 694, 829
BssNI GRCGYC 2 cut(s) 177, 703
BssSI CACGAG 1 cut(s) 158
Bst2BI CACGAG 1 cut(s) 158
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 6 cut(s) 454, 663, 753, 795, 827, 985
Bst6I CTCTTC 1 cut(s) 855
BstACI GRCGYC 2 cut(s) 177, 703
BstAPI GCANNNNNTGC 1 cut(s) 400
BstAUI TGTACA 1 cut(s) 1050
BstBAI YACGTR 1 cut(s) 111
BstC8I GCNNGC 5 cut(s) 289, 396, 459, 474, 634
BstDEI CTNAG 2 cut(s) 494, 1023
BstENI CCTNNNNNAGG 1 cut(s) 278
BstF5I GGATG 6 cut(s) 10, 180, 191, 213, 534, 601
BstFNI CGCG 7 cut(s) 78, 368, 405, 503, 606, 766, 857
BstHHI GCGC 5 cut(s) 80, 405, 606, 766, 946
BstKTI GATC 6 cut(s) 10, 105, 564, 625, 697, 832
BstMAI GTCTC 3 cut(s) 34, 921, 974
BstMBI GATC 6 cut(s) 7, 102, 561, 622, 694, 829
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 7 cut(s) 345, 385, 391, 400, 517, 854, 1107
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 207, 551
BstSLI GKGCMC 3 cut(s) 336, 381, 876
BstUI CGCG 7 cut(s) 78, 368, 405, 503, 606, 766, 857
BstV1I GCAGC 5 cut(s) 372, 477, 528, 773, 936
BstXI CCANNNNNNTGG 1 cut(s) 1066
BsuRI GGCC 8 cut(s) 284, 334, 379, 472, 551, 863, 939, 1058
BtsCI GGATG 6 cut(s) 10, 180, 191, 213, 534, 601
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 1 cut(s) 730
BtuMI TCGCGA 1 cut(s) 503
Cac8I GCNNGC 5 cut(s) 289, 396, 459, 474, 634
CaiI CAGNNNCTG 1 cut(s) 895
CciI TCATGA 1 cut(s) 10
CfoI GCGC 5 cut(s) 80, 405, 606, 766, 946
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 7 cut(s) 22, 332, 333, 377, 378, 471, 659
CseI GACGC 2 cut(s) 166, 692
Csp6I GTAC 2 cut(s) 717, 1051
CviAII CATG 3 cut(s) 11, 941, 1054
CviQI GTAC 2 cut(s) 717, 1051
DdeI CTNAG 2 cut(s) 494, 1023
DpnI GATC 6 cut(s) 9, 104, 563, 624, 696, 831
DpnII GATC 6 cut(s) 7, 102, 561, 622, 694, 829
DraIII CACNNNGTG 1 cut(s) 400
EaeI YGGCCR 2 cut(s) 861, 1056
Eam1104I CTCTTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 855
EciI GGCGGA 4 cut(s) 187, 476, 497, 626
Eco24I GRGCYC 2 cut(s) 336, 381
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 2 cut(s) 22, 659
Eco57I CTGAAG 1 cut(s) 410
Eco72I CACGTG 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoO109I RGGNCCY 3 cut(s) 332, 333, 377
EcoRI GAATTC 1 cut(s) 902
EcoRII CCWGG 1 cut(s) 207
EcoRV GATATC 1 cut(s) 1081
EcoT38I GRGCYC 2 cut(s) 336, 381
Esp3I CGTCTC 2 cut(s) 921, 974
FaeI CATG 3 cut(s) 14, 944, 1057
FaiI YATR 6 cut(s) 12, 837, 942, 1055, 1067, 1074
FaqI GGGAC 1 cut(s) 99
FatI CATG 3 cut(s) 10, 940, 1053
FauI CCCGC 6 cut(s) 260, 296, 344, 401, 481, 565
FbaI TGATCA 2 cut(s) 7, 829
FblI GTMKAC 1 cut(s) 706
FokI GGATG 6 cut(s) 17, 167, 178, 200, 541, 608
FriOI GRGCYC 2 cut(s) 336, 381
FspBI CTAG 2 cut(s) 734, 911
GlaI GCGC 5 cut(s) 79, 404, 605, 765, 945
GsaI CCCAGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 566
HaeIII GGCC 8 cut(s) 284, 334, 379, 472, 551, 863, 939, 1058
HapII CCGG 3 cut(s) 174, 521, 552
HgaI GACGC 2 cut(s) 166, 692
HhaI GCGC 5 cut(s) 80, 405, 606, 766, 946
Hin1I GRCGYC 2 cut(s) 177, 703
Hin1II CATG 3 cut(s) 14, 944, 1057
Hin6I GCGC 5 cut(s) 78, 403, 604, 764, 944
HinP1I GCGC 5 cut(s) 78, 403, 604, 764, 944
HincII GTYRAC 2 cut(s) 316, 707
HindII GTYRAC 2 cut(s) 316, 707
HinfI GANTC 4 cut(s) 498, 678, 768, 920
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 3 cut(s) 174, 521, 552
HphI GGTGA 3 cut(s) 79, 85, 436
Hpy166II GTNNAC 2 cut(s) 316, 707
Hpy188I TCNGA 5 cut(s) 34, 47, 265, 622, 891
Hpy188III TCNNGA 6 cut(s) 11, 160, 439, 502, 803, 924
Hpy8I GTNNAC 2 cut(s) 316, 707
Hpy99I CGWCG 5 cut(s) 122, 508, 667, 708, 711
HpyAV CCTTC 7 cut(s) 133, 214, 256, 331, 668, 800, 929
HpyCH4III ACNGT 6 cut(s) 454, 663, 753, 795, 827, 985
HpyCH4IV ACGT 3 cut(s) 84, 110, 312
HpyCH4V TGCA 1 cut(s) 952
HpyF10VI GCNNNNNNNGC 7 cut(s) 345, 385, 391, 400, 517, 854, 1107
HpyF3I CTNAG 2 cut(s) 494, 1023
HpySE526I ACGT 3 cut(s) 84, 110, 312
Hsp92I GRCGYC 2 cut(s) 177, 703
Hsp92II CATG 3 cut(s) 14, 944, 1057
HspAI GCGC 5 cut(s) 78, 403, 604, 764, 944
Ksp22I TGATCA 2 cut(s) 7, 829
KspAI GTTAAC 1 cut(s) 316
Kzo9I GATC 6 cut(s) 7, 102, 561, 622, 694, 829
LguI GCTCTTC 1 cut(s) 855
LmnI GCTCC 3 cut(s) 260, 487, 809
Lsp1109I GCAGC 5 cut(s) 372, 477, 528, 773, 936
LweI GCATC 4 cut(s) 456, 577, 882, 961
MaeI CTAG 2 cut(s) 734, 911
MaeII ACGT 3 cut(s) 84, 110, 312
MaeIII GTNAC 2 cut(s) 85, 971
MalI GATC 6 cut(s) 9, 104, 563, 624, 696, 831
MbiI CCGCTC 2 cut(s) 255, 601
MboI GATC 6 cut(s) 7, 102, 561, 622, 694, 829
MboII GAAGA 2 cut(s) 316, 842
MhlI GDGCHC 3 cut(s) 336, 381, 876
MlsI TGGCCA 1 cut(s) 1058
MluCI AATT 3 cut(s) 535, 737, 902
MluNI TGGCCA 1 cut(s) 1058
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 288
Mox20I TGGCCA 1 cut(s) 1058
MscI TGGCCA 1 cut(s) 1058
MseI TTAA 3 cut(s) 315, 534, 867
MslI CAYNNNNRTG 1 cut(s) 1064
Msp20I TGGCCA 1 cut(s) 1058
MspA1I CMGCKG 2 cut(s) 308, 949
MspI CCGG 3 cut(s) 174, 521, 552
MspR9I CCNGG 2 cut(s) 209, 553
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 7 cut(s) 78, 368, 405, 503, 606, 766, 857
MwoI GCNNNNNNNGC 7 cut(s) 345, 385, 391, 400, 517, 854, 1107
NciI CCSGG 1 cut(s) 553
NdeII GATC 6 cut(s) 7, 102, 561, 622, 694, 829
NlaIII CATG 3 cut(s) 14, 944, 1057
NlaIV GGNNCC 5 cut(s) 333, 334, 335, 378, 379
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 85
NruI TCGCGA 1 cut(s) 503
PagI TCATGA 1 cut(s) 10
PciSI GCTCTTC 1 cut(s) 855
PfeI GAWTC 3 cut(s) 678, 768, 920
PleI GAGTC 1 cut(s) 506
PmaCI CACGTG 1 cut(s) 111
PmlI CACGTG 1 cut(s) 111
PpsI GAGTC 1 cut(s) 506
Ppu21I YACGTR 1 cut(s) 111
PshBI ATTAAT 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 207
PspCI CACGTG 1 cut(s) 111
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 5 cut(s) 333, 334, 335, 378, 379
PspOMI GGGCCC 2 cut(s) 332, 377
PspPI GGNCC 7 cut(s) 22, 332, 333, 377, 378, 471, 659
PstNI CAGNNNCTG 1 cut(s) 895
RruI TCGCGA 1 cut(s) 503
RsaI GTAC 2 cut(s) 718, 1052
RsaNI GTAC 2 cut(s) 717, 1051
RseI CAYNNNNRTG 1 cut(s) 1064
SalI GTCGAC 1 cut(s) 705
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 3 cut(s) 315, 534, 867
Sau3AI GATC 6 cut(s) 7, 102, 561, 622, 694, 829
Sau96I GGNCC 7 cut(s) 22, 332, 333, 377, 378, 471, 659
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 2 cut(s) 209, 553
SduI GDGCHC 3 cut(s) 336, 381, 876
SfaNI GCATC 4 cut(s) 456, 577, 882, 961
SgrAI CRCCGGYG 1 cut(s) 173
SgrDI CGTCGACG 1 cut(s) 705
SinI GGWCC 2 cut(s) 22, 659
SmiMI CAYNNNNRTG 1 cut(s) 1064
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 3 cut(s) 535, 737, 902
SspI AATATT 1 cut(s) 433
SspMI CTAG 2 cut(s) 734, 911
StyD4I CCNGG 2 cut(s) 207, 551
TaaI ACNGT 6 cut(s) 454, 663, 753, 795, 827, 985
TaiI ACGT 3 cut(s) 87, 113, 315
TaqI TCGA 5 cut(s) 564, 665, 706, 804, 900
TasI AATT 3 cut(s) 535, 737, 902
TatI WGTACW 1 cut(s) 1050
TfiI GAWTC 3 cut(s) 678, 768, 920
Tru1I TTAA 3 cut(s) 315, 534, 867
Tru9I TTAA 3 cut(s) 315, 534, 867
TscAI CASTG 1 cut(s) 730
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 5 cut(s) 385, 490, 541, 761, 949
Tsp45I GTSAC 1 cut(s) 85
TspRI CASTG 1 cut(s) 730
VpaK11BI GGWCC 2 cut(s) 22, 659
VspI ATTAAT 1 cut(s) 534
XagI CCTNNNNNAGG 1 cut(s) 278
XapI RAATTY 1 cut(s) 902
XmiI GTMKAC 1 cut(s) 706
XspI CTAG 2 cut(s) 734, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.