Rroxscaffold_4G00286170

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016395
Physical Location & Seq
Reverse (-)
7307996 .. 7310907
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286170.1

Sequence Viewer

Length: 1083 bp
ATGGGGCCTATTTTGGAAGGGATTTTCCGACGAGAAATTGGCAAGTCGCAAAGGCGAGAAAATGGAGAGTCCAGACCTCCTCCCCTCACAAGTTCCTCGTCAAGCCCCCCACCGGCCTCATCCCTCCCTTATCCTTCGCCAACTTCCAGGTCGTCCTCAAGCCCCAGTCCCACCTCCCCGCCTCCTTCCCCCGCTCCCCTCCGACCGCTTCCTCATCAAGACCGCCGAGTTCTTCCCCTCCGCCGGCAACTCCGACTCCCTCGACTCCTGGTTCAGGGGGCCCACACTCGTGACCTCAAGCTCAAGGTCGCCTTCGTGGGCCCCGTTCTTCTGCGGCACGCCGTGCGCGGCGGGGATTGTGAGGCGGTCAGGAATATAATCAGGCGGCAGAGGACGATTCTGGCGGAGCTGACGCCCAGCGAGGCGGAGTCGCTGCTCCGAGTCGCGACCGAGTTGGCCGAGCCGGAGAGGATGATTAATTTGCTGCTTGAGGCCGGGTTGAGGATCGAGGCGGGTCTGAGGTTGGATCAGGTGGATGAGGAATTGGTGCGGAACGGGATGGATGAGCTACGCGTGGCGGAGGCTCCTGATCGGTTTGCTAGTGTTCTGGGTTTGAGGGAATCGGGACCGTCGACTGCAGAAGACCCCAGGATAACTAAAGGTCATGGCCATCGTCCACGAGTTGCTACTGATAGTGATAAGCTAGAATTGGGGGAACAAATGTTGATGGCAGCGCGAATCGGGGATTTGAGAAATGTGGAACTGTTGTTTCTTGAAGGAGCAGACATTAGCTACTGTGATCAATATGGCTTGACAGCTCTTCATGCGGCGGCCATTAAAGGGCACAAGGAGGTTGTTCTGATGCTGATCGAATTTGGGCTAGACTTGGAATCCAGAGACAGTGAAGGCCATGCGCCGCTGCATTTGGCGGTTGTGGGGGGCAACTTGGAGACGGTTGAGATGTTGATACAGAATGGGGCTAATGTGAATGCCAAGAGCAAGAGTGGGGCTACTCCTTTGTACATGGCCACAGCTATGGGGTATGATGATATCACTGAGTTTCTTATCAGCAGAGGAGGCTGA

Protein Analysis

360

Amino Acids

39.21

Weight (kDa)

6.34

Isoelectric Point (pI)

63.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 243 - 290 1.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 243 - 332 4e-20 Ankyrin repeats (3 copies)
Ank_5 PF13857 264 - 310 3.8e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 274 - 323 2.3e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 289 - 340 5.3e-06 Ankyrin repeats (many copies)
Ank PF00023 303 - 333 6.9e-08 Ankyrin repeat
Ank_4 PF13637 307 - 356 9.9e-10 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016395)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24970 FvH4_7g24970
malus_domestica MD01G1157100.v1.1
prunus_persica Prupe.2G254900_v2.0.a1
pyrus_communis pycom01g17390
rosa_chinensis RchiOBHm_Chr1g0370871
rosa_laevigata RLG00000027003
rosa_multiflora Rmu_sc0009474.1_g000017
rosa_roxburghii Rroxscaffold_4G00286170
rosa_rugosa Rorug01G0360000
rosa_samantha Rh1AG369700 Rh1BG333300 Rh1CG346700 Rh1DG364500
rosa_wichuraiana Rw1G032550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 632
AccII CGCG 4 cut(s) 348, 446, 573, 736
AclWI GGATC 2 cut(s) 512, 534
AcoI YGGCCR 4 cut(s) 456, 667, 831, 1026
AcsI RAATTY 1 cut(s) 872
AcyI GRCGYC 1 cut(s) 413
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 1 cut(s) 1022
AfiI CCNNNNNNNGG 5 cut(s) 112, 243, 274, 501, 840
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 1 cut(s) 776
AjnI CCWGG 3 cut(s) 146, 267, 647
AleI CACNNNNGTG 1 cut(s) 288
AluBI AGCT 7 cut(s) 301, 409, 568, 703, 792, 818, 1034
AluI AGCT 7 cut(s) 301, 409, 568, 703, 792, 818, 1034
Alw26I GTCTC 2 cut(s) 891, 944
AlwI GGATC 2 cut(s) 512, 534
ApaI GGGCCC 2 cut(s) 283, 323
ApeKI GCWGC 4 cut(s) 433, 484, 731, 919
ApoI RAATTY 1 cut(s) 872
AseI ATTAAT 1 cut(s) 477
AspLEI GCGC 3 cut(s) 348, 736, 916
AspS9I GGNCC 6 cut(s) 5, 279, 280, 319, 320, 626
AsuC2I CCSGG 1 cut(s) 496
AvaII GGWCC 1 cut(s) 626
BaeGI GKGCMC 3 cut(s) 283, 323, 846
BalI TGGCCA 2 cut(s) 669, 1028
BanII GRGCYC 2 cut(s) 283, 323
BauI CACGAG 2 cut(s) 288, 678
BbsI GAAGAC 1 cut(s) 648
BbvI GCAGC 4 cut(s) 420, 471, 743, 906
BccI CCATC 3 cut(s) 553, 678, 721
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 3 cut(s) 148, 269, 649
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 2 cut(s) 891, 944
BfaI CTAG 3 cut(s) 600, 704, 881
BfmI CTRYAG 1 cut(s) 636
Bme1390I CCNGG 4 cut(s) 148, 269, 496, 649
Bme18I GGWCC 1 cut(s) 626
BmgT120I GGNCC 6 cut(s) 5, 279, 280, 319, 320, 626
BmiI GGNNCC 7 cut(s) 6, 280, 281, 321, 322, 585, 627
BmrFI CCNGG 4 cut(s) 148, 269, 496, 649
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 852
BmuI ACTGGG 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 648
BpuEI CTTGAG 4 cut(s) 142, 281, 287, 509
BpuMI CCSGG 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 866
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 647
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 243, 274, 501, 840
Bse118I RCCGGY 2 cut(s) 112, 243
Bse1I ACTGG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 866
BseBI CCWGG 3 cut(s) 148, 269, 649
BseDI CCNNGG 1 cut(s) 647
BseGI GGATG 5 cut(s) 119, 477, 541, 564, 568
BseJI GATNNNNATC 1 cut(s) 866
BseLI CCNNNNNNNGG 5 cut(s) 112, 243, 274, 501, 840
BseMII CTCAG 2 cut(s) 509, 1047
BseNI ACTGG 1 cut(s) 165
BseRI GAGGAG 1 cut(s) 69
BseSI GKGCMC 3 cut(s) 283, 323, 846
BseXI GCAGC 4 cut(s) 420, 471, 743, 906
BseYI CCCAGC 1 cut(s) 416
Bsh1236I CGCG 4 cut(s) 348, 446, 573, 736
Bsh1285I CGRYCG 2 cut(s) 206, 450
BsiEI CGRYCG 2 cut(s) 206, 450
BsiSI CCGG 4 cut(s) 113, 244, 464, 495
BslFI GGGAC 2 cut(s) 153, 639
BslI CCNNNNNNNGG 5 cut(s) 112, 243, 274, 501, 840
BsmAI GTCTC 2 cut(s) 891, 944
BsmBI CGTCTC 1 cut(s) 944
BsmFI GGGAC 2 cut(s) 153, 639
BsmI GAATGC 1 cut(s) 994
Bsp120I GGGCCC 2 cut(s) 279, 319
Bsp1286I GDGCHC 3 cut(s) 283, 323, 846
Bsp1407I TGTACA 1 cut(s) 1020
Bsp143I GATC 5 cut(s) 504, 526, 589, 799, 867
Bsp68I TCGCGA 1 cut(s) 446
BspCNI CTCAG 2 cut(s) 510, 1048
BspFNI CGCG 4 cut(s) 348, 446, 573, 736
BspLI GGNNCC 7 cut(s) 6, 280, 281, 321, 322, 585, 627
BspMAI CTGCAG 1 cut(s) 640
BspPI GGATC 2 cut(s) 512, 534
BspQI GCTCTTC 1 cut(s) 825
BsrBI CCGCTC 1 cut(s) 194
BsrFI RCCGGY 2 cut(s) 112, 243
BsrGI TGTACA 1 cut(s) 1020
BsrI ACTGG 1 cut(s) 165
BssAI RCCGGY 2 cut(s) 112, 243
BssECI CCNNGG 1 cut(s) 647
BssMI GATC 5 cut(s) 504, 526, 589, 799, 867
BssNI GRCGYC 1 cut(s) 413
BssSI CACGAG 2 cut(s) 288, 678
Bst2BI CACGAG 2 cut(s) 288, 678
Bst2UI CCWGG 3 cut(s) 148, 269, 649
Bst4CI ACNGT 5 cut(s) 630, 765, 797, 902, 955
Bst6I CTCTTC 1 cut(s) 825
BstACI GRCGYC 1 cut(s) 413
BstAPI GCANNNNNTGC 1 cut(s) 343
BstAUI TGTACA 1 cut(s) 1020
BstC8I GCNNGC 2 cut(s) 245, 339
BstDEI CTNAG 2 cut(s) 518, 1056
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 5 cut(s) 119, 477, 541, 564, 568
BstFNI CGCG 4 cut(s) 348, 446, 573, 736
BstHHI GCGC 3 cut(s) 348, 736, 916
BstKTI GATC 5 cut(s) 507, 529, 592, 802, 870
BstMAI GTCTC 2 cut(s) 891, 944
BstMBI GATC 5 cut(s) 504, 526, 589, 799, 867
BstMCI CGRYCG 2 cut(s) 206, 450
BstMWI GCNNNNNNNGC 3 cut(s) 343, 824, 1077
BstNI CCWGG 3 cut(s) 148, 269, 649
BstSCI CCNGG 4 cut(s) 146, 267, 494, 647
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 3 cut(s) 283, 323, 846
BstUI CGCG 4 cut(s) 348, 446, 573, 736
BstV1I GCAGC 4 cut(s) 420, 471, 743, 906
BstV2I GAAGAC 1 cut(s) 648
BstXI CCANNNNNNTGG 1 cut(s) 1036
BtsCI GGATG 5 cut(s) 119, 477, 541, 564, 568
BtsIMutI CAGTG 2 cut(s) 907, 1053
BtuMI TCGCGA 1 cut(s) 446
Cac8I GCNNGC 2 cut(s) 245, 339
CfoI GCGC 3 cut(s) 348, 736, 916
Cfr10I RCCGGY 2 cut(s) 112, 243
Cfr13I GGNCC 6 cut(s) 5, 279, 280, 319, 320, 626
CseI GACGC 1 cut(s) 421
Csp6I GTAC 1 cut(s) 1021
CviAII CATG 4 cut(s) 665, 824, 911, 1024
CviQI GTAC 1 cut(s) 1021
DdeI CTNAG 2 cut(s) 518, 1056
DpnI GATC 5 cut(s) 506, 528, 591, 801, 869
DpnII GATC 5 cut(s) 504, 526, 589, 799, 867
DraIII CACNNNGTG 1 cut(s) 343
EaeI YGGCCR 4 cut(s) 456, 667, 831, 1026
Eam1104I CTCTTC 1 cut(s) 825
EarI CTCTTC 1 cut(s) 825
EciI GGCGGA 4 cut(s) 230, 419, 440, 593
Eco24I GRGCYC 2 cut(s) 283, 323
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 626
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 3 cut(s) 5, 279, 320
EcoRII CCWGG 3 cut(s) 146, 267, 647
EcoRV GATATC 1 cut(s) 1051
EcoT38I GRGCYC 2 cut(s) 283, 323
Esp3I CGTCTC 1 cut(s) 944
FaeI CATG 4 cut(s) 668, 827, 914, 1027
FaiI YATR 8 cut(s) 377, 666, 807, 825, 912, 1025, 1037, 1044
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 153, 639
FatI CATG 4 cut(s) 664, 823, 910, 1023
FauI CCCGC 4 cut(s) 186, 199, 344, 505
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 1 cut(s) 632
FokI GGATG 5 cut(s) 106, 484, 548, 571, 575
FriOI GRGCYC 2 cut(s) 283, 323
FspBI CTAG 3 cut(s) 600, 704, 881
GlaI GCGC 3 cut(s) 347, 735, 915
GsaI CCCAGC 1 cut(s) 420
HapII CCGG 4 cut(s) 113, 244, 464, 495
HgaI GACGC 1 cut(s) 421
HhaI GCGC 3 cut(s) 348, 736, 916
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 4 cut(s) 668, 827, 914, 1027
Hin6I GCGC 3 cut(s) 346, 734, 914
HinP1I GCGC 3 cut(s) 346, 734, 914
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HinfI GANTC 9 cut(s) 68, 255, 264, 397, 428, 441, 620, 738, 890
HpaII CCGG 4 cut(s) 113, 244, 464, 495
Hpy166II GTNNAC 2 cut(s) 633, 677
Hpy188I TCNGA 6 cut(s) 29, 203, 254, 440, 519, 861
Hpy188III TCNNGA 9 cut(s) 72, 218, 290, 370, 445, 587, 624, 773, 894
Hpy8I GTNNAC 2 cut(s) 633, 677
Hpy99I CGWCG 2 cut(s) 33, 634
HpyAV CCTTC 6 cut(s) 11, 144, 195, 322, 770, 899
HpyCH4III ACNGT 5 cut(s) 630, 765, 797, 902, 955
HpyCH4V TGCA 2 cut(s) 638, 922
HpyF10VI GCNNNNNNNGC 3 cut(s) 343, 824, 1077
HpyF3I CTNAG 2 cut(s) 518, 1056
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 4 cut(s) 668, 827, 914, 1027
HspAI GCGC 3 cut(s) 346, 734, 914
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 799
Kzo9I GATC 5 cut(s) 504, 526, 589, 799, 867
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 5 cut(s) 199, 406, 441, 589, 779
Lsp1109I GCAGC 4 cut(s) 420, 471, 743, 906
LweI GCATC 1 cut(s) 852
MaeI CTAG 3 cut(s) 600, 704, 881
MaeIII GTNAC 1 cut(s) 290
MalI GATC 5 cut(s) 506, 528, 591, 801, 869
MbiI CCGCTC 1 cut(s) 194
MboI GATC 5 cut(s) 504, 526, 589, 799, 867
MboII GAAGA 4 cut(s) 224, 320, 653, 812
MhlI GDGCHC 3 cut(s) 283, 323, 846
MlsI TGGCCA 2 cut(s) 669, 1028
MluCI AATT 5 cut(s) 36, 478, 542, 707, 872
MluI ACGCGT 1 cut(s) 571
MluNI TGGCCA 2 cut(s) 669, 1028
MlyI GAGTC 5 cut(s) 77, 249, 258, 437, 450
MmeI TCCRAC 4 cut(s) 52, 226, 277, 504
Mox20I TGGCCA 2 cut(s) 669, 1028
MroNI GCCGGC 1 cut(s) 243
MscI TGGCCA 2 cut(s) 669, 1028
MseI TTAA 2 cut(s) 477, 837
MslI CAYNNNNRTG 2 cut(s) 288, 1034
Msp20I TGGCCA 2 cut(s) 669, 1028
MspA1I CMGCKG 1 cut(s) 919
MspI CCGG 4 cut(s) 113, 244, 464, 495
MspR9I CCNGG 4 cut(s) 148, 269, 496, 649
Mva1269I GAATGC 1 cut(s) 994
MvaI CCWGG 3 cut(s) 148, 269, 649
MvnI CGCG 4 cut(s) 348, 446, 573, 736
MwoI GCNNNNNNNGC 3 cut(s) 343, 824, 1077
NaeI GCCGGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 496
NdeII GATC 5 cut(s) 504, 526, 589, 799, 867
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 4 cut(s) 668, 827, 914, 1027
NlaIV GGNNCC 7 cut(s) 6, 280, 281, 321, 322, 585, 627
NmeAIII GCCGAG 2 cut(s) 251, 484
NmuCI GTSAC 1 cut(s) 290
NruI TCGCGA 1 cut(s) 446
OliI CACNNNNGTG 1 cut(s) 288
PciSI GCTCTTC 1 cut(s) 825
PcsI WCGNNNNNNNCGW 2 cut(s) 321, 629
PctI GAATGC 1 cut(s) 994
PdiI GCCGGC 1 cut(s) 245
PfeI GAWTC 4 cut(s) 397, 620, 738, 890
PleI GAGTC 5 cut(s) 76, 249, 258, 436, 449
PpsI GAGTC 5 cut(s) 76, 249, 258, 436, 449
PshBI ATTAAT 1 cut(s) 477
Psp6I CCWGG 3 cut(s) 146, 267, 647
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 3 cut(s) 146, 267, 647
PspN4I GGNNCC 7 cut(s) 6, 280, 281, 321, 322, 585, 627
PspOMI GGGCCC 2 cut(s) 279, 319
PspPI GGNCC 6 cut(s) 5, 279, 280, 319, 320, 626
PstI CTGCAG 1 cut(s) 640
RruI TCGCGA 1 cut(s) 446
RsaI GTAC 1 cut(s) 1022
RsaNI GTAC 1 cut(s) 1021
RseI CAYNNNNRTG 2 cut(s) 288, 1034
SalI GTCGAC 1 cut(s) 631
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 2 cut(s) 477, 837
Sau3AI GATC 5 cut(s) 504, 526, 589, 799, 867
Sau96I GGNCC 6 cut(s) 5, 279, 280, 319, 320, 626
SchI GAGTC 5 cut(s) 77, 249, 258, 437, 450
ScrFI CCNGG 4 cut(s) 148, 269, 496, 649
SduI GDGCHC 3 cut(s) 283, 323, 846
SfaNI GCATC 1 cut(s) 852
SfcI CTRYAG 1 cut(s) 636
SinI GGWCC 1 cut(s) 626
SmiMI CAYNNNNRTG 2 cut(s) 288, 1034
SmlI CTYRAG 4 cut(s) 157, 296, 302, 488
SmoI CTYRAG 4 cut(s) 157, 296, 302, 488
Sse9I AATT 5 cut(s) 36, 478, 542, 707, 872
SspMI CTAG 3 cut(s) 600, 704, 881
StyD4I CCNGG 4 cut(s) 146, 267, 494, 647
TaaI ACNGT 5 cut(s) 630, 765, 797, 902, 955
TaqI TCGA 4 cut(s) 262, 507, 632, 870
TaqII GACCGA 1 cut(s) 464
TasI AATT 5 cut(s) 36, 478, 542, 707, 872
TatI WGTACW 1 cut(s) 1020
TauI GCSGC 6 cut(s) 337, 351, 388, 830, 833, 919
TfiI GAWTC 4 cut(s) 397, 620, 738, 890
Tru1I TTAA 2 cut(s) 477, 837
Tru9I TTAA 2 cut(s) 477, 837
TscAI CASTG 2 cut(s) 907, 1060
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 4 cut(s) 433, 484, 731, 919
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 812
TspRI CASTG 2 cut(s) 907, 1060
VpaK11BI GGWCC 1 cut(s) 626
VspI ATTAAT 1 cut(s) 477
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 632
XspI CTAG 3 cut(s) 600, 704, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.