MD01G1157100.v1.1

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016395
Physical Location & Seq
Forward (+)
26439095 .. 26441038
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1157100.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGCCAATCTCCTCCTCCTCCCTCCTCCCTCCACCCATAAGCTCCTCCTCTTTCATTCGGATAATCCCACCTTCTCAATCCAGTCCCGTCCAATCCAGTCCAGTCTGTATTCCGATGGATAAAAAACTAGTACAAGTTTCGGAGCAAGAAATTTGTATCGACTTCGCACTCGACTCCAAATGCCGCGCTAACGTGCGCCTTACCTCCCTATGCGCCACCGCCGCCGTAGCCTTCAAGGTCCAAACCTCCTCCCCTCACAAGTTCCTCGTCAAACCGCCCACCGGCTTAATCCCGCCTCTCTCCCACGTCACCTTCCAGGTCATCCTCAAGCCCCAGATCCAGTTCCCGCCCGCCTACCCTCGCTCGCCGTCCGACAAATTCCTTATAAGAACCGCAGAGTTCACCACCGAGTTAACTCACTCCGACTCCATCAACCACTGGTTCTCCAGCTCTTCGTGTCCCCGCGGGTCCACTCACGATATCAAGCTCAAGGTCGCCTTTGTGGGCCCCTTGCTGTTGCGCCACGCCGTCACTTGCGGGGACTACGACGGCGTCAGGAATATAATCAAGCGGCAGAGGACGATTCTCGCCGAGTTGCCGCCGGCGGAAGCTGAGTCGATCCTCCGGATTGCGACCGAGTTGGATAACCCGGAGAACTTGATTAACTTACTGCTCGAAGGGGGATTGAGGATTGACCCGATCAGCGATCAAGTGCATTACGGCGGAGATTCTAAATGTCCATCCAACGGACGCGATGAGCTAGACGTGGCAGGGGCGTGTGATCGACTGGATGATGTTCTGGGACTGAGGGAATCTGAGCCGTTGGATTCTAAGGACAGGGGAAATGGCAGGTGTGCCGAGGTGAGGAAATCTAGTGCGGACCCCACTACAACTATTGGCCACAGCCGTTCGCGCCATGATTTTGTTCATGACAAAGATAAACTAGAAATGGGAGAACTAATGTTGATGGCAGCGAGAAGAGGTGATTTGAAGAATGTTGAATTGTTGTTACAAAATGGCGCGGATATAAACTGCTGTGACCAATATGGCTTGACAGCCCTGCATGCCGCGGCCATCAAAGGCCACAAGGACGTTGCTCGGGTGCTCATCCGATTTGGGTCGGACTTGGAATCCCAAGACGGTGAAGGGCACGCACCCTTGCACATGGCCGTGGTCGGCGGGAGCTTAGAGACAGTTCAAATCTTGGTTCATGGTGGGGCTAACGTCAATGCCAAGAGCAACGGCGGCGCCACCCCTCTATACATGGCTACTGCTATGGGGTACGATGATATAAGTGAGTTTCTTACCAGCAGAAAACATTAA

Protein Analysis

441

Amino Acids

47.72

Weight (kDa)

6.24

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 58 - 149 1e-12 MSP (Major sperm protein) domain
Ank_4 PF13637 322 - 370 1.4e-12 Ankyrin repeats (many copies)
Ank_2 PF12796 323 - 412 7.9e-21 Ankyrin repeats (3 copies)
Ank_5 PF13857 336 - 390 1.6e-08 Ankyrin repeats (many copies)
Ank PF00023 349 - 380 4.9e-06 Ankyrin repeat
Ank_2 PF12796 373 - 436 3.1e-09 Ankyrin repeats (3 copies)
Ank PF00023 383 - 414 4.2e-06 Ankyrin repeat
Ank_4 PF13637 387 - 434 4.2e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016395)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24970 FvH4_7g24970
malus_domestica MD01G1157100.v1.1
prunus_persica Prupe.2G254900_v2.0.a1
pyrus_communis pycom01g17390
rosa_chinensis RchiOBHm_Chr1g0370871
rosa_laevigata RLG00000027003
rosa_multiflora Rmu_sc0009474.1_g000017
rosa_roxburghii Rroxscaffold_4G00286170
rosa_rugosa Rorug01G0360000
rosa_samantha Rh1AG369700 Rh1BG333300 Rh1CG346700 Rh1DG364500
rosa_wichuraiana Rw1G032550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 386
AarI CACCTGC 1 cut(s) 840
Acc36I ACCTGC 1 cut(s) 840
AccB1I GGYRCC 1 cut(s) 1247
AccII CGCG 6 cut(s) 186, 465, 753, 913, 1022, 1070
AccIII TCCGGA 1 cut(s) 624
AclWI GGATC 2 cut(s) 331, 613
AcoI YGGCCR 3 cut(s) 898, 1071, 1167
AcsI RAATTY 2 cut(s) 150, 377
AcyI GRCGYC 2 cut(s) 552, 1248
AfaI GTAC 2 cut(s) 132, 1283
AfiI CCNNNNNNNGG 3 cut(s) 281, 746, 864
AgsI TTSAA 4 cut(s) 235, 991, 1001, 1199
AhlI ACTAGT 1 cut(s) 127
AjiI CACGTC 2 cut(s) 307, 766
AjnI CCWGG 1 cut(s) 315
AluBI AGCT 6 cut(s) 42, 450, 487, 611, 760, 1185
AluI AGCT 6 cut(s) 42, 450, 487, 611, 760, 1185
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 1 cut(s) 1184
AlwI GGATC 2 cut(s) 331, 613
Ama87I CYCGRG 1 cut(s) 1098
Aor13HI TCCGGA 1 cut(s) 624
AoxI GGCC 5 cut(s) 505, 898, 1071, 1081, 1167
ApaI GGGCCC 1 cut(s) 509
ApeKI GCWGC 1 cut(s) 971
ApoI RAATTY 2 cut(s) 150, 377
AspLEI GCGC 7 cut(s) 188, 198, 215, 522, 915, 1022, 1250
AspS9I GGNCC 5 cut(s) 238, 468, 505, 506, 880
AsuC2I CCSGG 1 cut(s) 650
AsuHPI GGTGA 5 cut(s) 301, 394, 874, 995, 1154
AvaI CYCGRG 1 cut(s) 1098
AvaII GGWCC 3 cut(s) 238, 468, 880
BaeGI GKGCMC 2 cut(s) 509, 1152
BalI TGGCCA 1 cut(s) 900
BanI GGYRCC 1 cut(s) 1247
BanII GRGCYC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 1 cut(s) 983
BccI CCATC 5 cut(s) 109, 437, 748, 961, 1082
BceAI ACGGC 9 cut(s) 209, 352, 512, 565, 736, 805, 891, 1154, 1258
BciT130I CCWGG 1 cut(s) 317
BcnI CCSGG 1 cut(s) 650
BcoDI GTCTC 1 cut(s) 1184
BcuI ACTAGT 1 cut(s) 127
BfaI CTAG 4 cut(s) 128, 761, 873, 944
BfoI RGCGCY 1 cut(s) 1251
BfuAI ACCTGC 1 cut(s) 840
BisI GCNGC 8 cut(s) 184, 222, 572, 599, 972, 1068, 1071, 1246
BlsI GCNGC 8 cut(s) 185, 223, 573, 600, 973, 1069, 1072, 1247
Bme1390I CCNGG 2 cut(s) 317, 650
Bme18I GGWCC 3 cut(s) 238, 468, 880
BmeT110I CYCGRG 1 cut(s) 1098
BmgBI CACGTC 2 cut(s) 307, 766
BmgT120I GGNCC 5 cut(s) 238, 468, 505, 506, 880
BmiI GGNNCC 5 cut(s) 469, 507, 508, 882, 1249
BmrFI CCNGG 2 cut(s) 317, 650
BpmI CTGGAG 1 cut(s) 430
BpuEI CTTGAG 2 cut(s) 311, 473
BpuMI CCSGG 1 cut(s) 650
BsaHI GRCGYC 2 cut(s) 552, 1248
BsaJI CCNNGG 4 cut(s) 463, 858, 1068, 1170
BsaWI WCCGGW 1 cut(s) 624
BsaXI ACNNNNNCTCC 4 cut(s) 410, 428, 440, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 746, 864
Bse118I RCCGGY 2 cut(s) 281, 601
Bse1I ACTGG 6 cut(s) 81, 96, 101, 340, 443, 792
BseAI TCCGGA 1 cut(s) 624
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 4 cut(s) 463, 858, 1068, 1170
BseGI GGATG 4 cut(s) 321, 740, 796, 1107
BseLI CCNNNNNNNGG 3 cut(s) 281, 746, 864
BseMII CTCAG 3 cut(s) 603, 797, 807
BseNI ACTGG 6 cut(s) 81, 96, 101, 340, 443, 792
BseRI GAGGAG 6 cut(s) 4, 7, 14, 34, 37, 238
BseSI GKGCMC 2 cut(s) 509, 1152
BseXI GCAGC 1 cut(s) 983
Bsh1236I CGCG 6 cut(s) 186, 465, 753, 913, 1022, 1070
Bsh1285I CGRYCG 1 cut(s) 636
BshFI GGCC 5 cut(s) 507, 900, 1073, 1083, 1169
BshNI GGYRCC 1 cut(s) 1247
BsiEI CGRYCG 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 1107
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 4 cut(s) 282, 602, 625, 650
BslFI GGGAC 4 cut(s) 69, 444, 554, 816
BslI CCNNNNNNNGG 3 cut(s) 281, 746, 864
BsmAI GTCTC 1 cut(s) 1184
BsmFI GGGAC 4 cut(s) 69, 444, 554, 816
BsnI GGCC 5 cut(s) 507, 900, 1073, 1083, 1169
BsoBI CYCGRG 1 cut(s) 1098
Bsp120I GGGCCC 1 cut(s) 505
Bsp1286I GDGCHC 3 cut(s) 509, 1107, 1152
Bsp13I TCCGGA 1 cut(s) 624
Bsp143I GATC 5 cut(s) 336, 618, 699, 706, 781
BspANI GGCC 5 cut(s) 507, 900, 1073, 1083, 1169
BspCNI CTCAG 3 cut(s) 604, 798, 808
BspEI TCCGGA 1 cut(s) 624
BspFNI CGCG 6 cut(s) 186, 465, 753, 913, 1022, 1070
BspHI TCATGA 1 cut(s) 928
BspLI GGNNCC 5 cut(s) 469, 507, 508, 882, 1249
BspMI ACCTGC 1 cut(s) 840
BspPI GGATC 2 cut(s) 331, 613
BspQI GCTCTTC 1 cut(s) 457
BspT107I GGYRCC 1 cut(s) 1247
BsrFI RCCGGY 2 cut(s) 281, 601
BsrI ACTGG 6 cut(s) 81, 96, 101, 340, 443, 792
BssAI RCCGGY 2 cut(s) 281, 601
BssECI CCNNGG 4 cut(s) 463, 858, 1068, 1170
BssMI GATC 5 cut(s) 336, 618, 699, 706, 781
BssNI GRCGYC 2 cut(s) 552, 1248
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 2 cut(s) 1142, 1195
Bst6I CTCTTC 2 cut(s) 457, 973
BstACI GRCGYC 2 cut(s) 552, 1248
BstC8I GCNNGC 5 cut(s) 351, 365, 603, 1065, 1152
BstDEI CTNAG 5 cut(s) 612, 806, 816, 831, 1186
BstDSI CCRYGG 3 cut(s) 463, 1068, 1170
BstF5I GGATG 4 cut(s) 321, 740, 796, 1107
BstFNI CGCG 6 cut(s) 186, 465, 753, 913, 1022, 1070
BstH2I RGCGCY 1 cut(s) 1251
BstHHI GCGC 7 cut(s) 188, 198, 215, 522, 915, 1022, 1250
BstKTI GATC 5 cut(s) 339, 621, 702, 709, 784
BstMAI GTCTC 1 cut(s) 1184
BstMBI GATC 5 cut(s) 336, 618, 699, 706, 781
BstMCI CGRYCG 1 cut(s) 636
BstMWI GCNNNNNNNGC 5 cut(s) 221, 227, 912, 1064, 1245
BstNI CCWGG 1 cut(s) 317
BstNSI RCATGY 1 cut(s) 1067
BstSCI CCNGG 2 cut(s) 315, 648
BstSLI GKGCMC 2 cut(s) 509, 1152
BstUI CGCG 6 cut(s) 186, 465, 753, 913, 1022, 1070
BstV1I GCAGC 1 cut(s) 983
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
BsuRI GGCC 5 cut(s) 507, 900, 1073, 1083, 1169
BtgI CCRYGG 3 cut(s) 463, 1068, 1170
BtgZI GCGATG 1 cut(s) 768
BtrI CACGTC 2 cut(s) 307, 766
BtsCI GGATG 4 cut(s) 321, 740, 796, 1107
BtsIMutI CAGTG 1 cut(s) 436
BveI ACCTGC 1 cut(s) 840
Cac8I GCNNGC 5 cut(s) 351, 365, 603, 1065, 1152
CciI TCATGA 1 cut(s) 928
CfoI GCGC 7 cut(s) 188, 198, 215, 522, 915, 1022, 1250
Cfr10I RCCGGY 2 cut(s) 281, 601
Cfr13I GGNCC 5 cut(s) 238, 468, 505, 506, 880
Cfr42I CCGCGG 2 cut(s) 466, 1071
CseI GACGC 2 cut(s) 541, 759
Csp6I GTAC 2 cut(s) 131, 1282
CviAII CATG 6 cut(s) 917, 929, 1064, 1165, 1211, 1264
CviQI GTAC 2 cut(s) 131, 1282
DdeI CTNAG 5 cut(s) 612, 806, 816, 831, 1186
DinI GGCGCC 1 cut(s) 1249
DpnI GATC 5 cut(s) 338, 620, 701, 708, 783
DpnII GATC 5 cut(s) 336, 618, 699, 706, 781
EaeI YGGCCR 3 cut(s) 898, 1071, 1167
Eam1104I CTCTTC 2 cut(s) 457, 973
EarI CTCTTC 2 cut(s) 457, 973
EciI GGCGGA 2 cut(s) 620, 738
Eco24I GRGCYC 1 cut(s) 509
Eco32I GATATC 1 cut(s) 481
Eco47I GGWCC 3 cut(s) 238, 468, 880
Eco88I CYCGRG 1 cut(s) 1098
EcoO109I RGGNCCY 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 315
EcoRV GATATC 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 509
EgeI GGCGCC 1 cut(s) 1249
EheI GGCGCC 1 cut(s) 1249
FaeI CATG 6 cut(s) 920, 932, 1067, 1168, 1214, 1267
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 4 cut(s) 69, 444, 554, 816
FatI CATG 6 cut(s) 916, 928, 1063, 1164, 1210, 1263
FauI CCCGC 7 cut(s) 300, 354, 358, 458, 470, 530, 1172
Fnu4HI GCNGC 8 cut(s) 184, 222, 572, 599, 972, 1068, 1071, 1246
FokI GGATG 4 cut(s) 308, 727, 803, 1094
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 8 cut(s) 184, 222, 572, 599, 972, 1068, 1071, 1246
FspBI CTAG 4 cut(s) 128, 761, 873, 944
GlaI GCGC 7 cut(s) 187, 197, 214, 521, 914, 1021, 1249
GluI GCNGC 8 cut(s) 184, 222, 572, 599, 972, 1068, 1071, 1246
GsuI CTGGAG 1 cut(s) 430
HaeII RGCGCY 1 cut(s) 1251
HaeIII GGCC 5 cut(s) 507, 900, 1073, 1083, 1169
HapII CCGG 4 cut(s) 282, 602, 625, 650
HgaI GACGC 2 cut(s) 541, 759
HhaI GCGC 7 cut(s) 188, 198, 215, 522, 915, 1022, 1250
Hin1I GRCGYC 2 cut(s) 552, 1248
Hin1II CATG 6 cut(s) 920, 932, 1067, 1168, 1214, 1267
Hin6I GCGC 7 cut(s) 186, 196, 213, 520, 913, 1020, 1248
HinP1I GCGC 7 cut(s) 186, 196, 213, 520, 913, 1020, 1248
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 8 cut(s) 173, 425, 583, 614, 728, 812, 827, 1130
HpaI GTTAAC 1 cut(s) 414
HpaII CCGG 4 cut(s) 282, 602, 625, 650
HphI GGTGA 5 cut(s) 301, 394, 874, 995, 1154
Hpy166II GTNNAC 3 cut(s) 402, 414, 471
Hpy188I TCNGA 8 cut(s) 60, 114, 142, 373, 424, 817, 1112, 1123
Hpy188III TCNNGA 4 cut(s) 476, 556, 625, 929
Hpy8I GTNNAC 3 cut(s) 402, 414, 471
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 5 cut(s) 81, 241, 322, 671, 1139
HpyCH4III ACNGT 2 cut(s) 1142, 1195
HpyCH4IV ACGT 5 cut(s) 192, 306, 765, 1092, 1224
HpyCH4V TGCA 3 cut(s) 715, 1063, 1162
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 227, 912, 1064, 1245
HpyF3I CTNAG 5 cut(s) 612, 806, 816, 831, 1186
HpySE526I ACGT 5 cut(s) 192, 306, 765, 1092, 1224
Hsp92I GRCGYC 2 cut(s) 552, 1248
Hsp92II CATG 6 cut(s) 920, 932, 1067, 1168, 1214, 1267
HspAI GCGC 7 cut(s) 186, 196, 213, 520, 913, 1020, 1248
KasI GGCGCC 1 cut(s) 1247
Kpn2I TCCGGA 1 cut(s) 624
KroI GCCGGC 1 cut(s) 601
KroNI GCCGGC 1 cut(s) 603
KspAI GTTAAC 1 cut(s) 414
KspI CCGCGG 2 cut(s) 466, 1071
Kzo9I GATC 5 cut(s) 336, 618, 699, 706, 781
LguI GCTCTTC 1 cut(s) 457
LmnI GCTCC 3 cut(s) 47, 142, 1182
Lsp1109I GCAGC 1 cut(s) 983
MaeI CTAG 4 cut(s) 128, 761, 873, 944
MaeII ACGT 5 cut(s) 192, 306, 765, 1092, 1224
MaeIII GTNAC 4 cut(s) 307, 529, 1008, 1037
MalI GATC 5 cut(s) 338, 620, 701, 708, 783
MboI GATC 5 cut(s) 336, 618, 699, 706, 781
MboII GAAGA 3 cut(s) 444, 990, 1003
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 3 cut(s) 509, 1107, 1152
MlsI TGGCCA 1 cut(s) 900
MluCI AATT 3 cut(s) 150, 377, 1001
MluNI TGGCCA 1 cut(s) 900
Mly113I GGCGCC 1 cut(s) 1248
MlyI GAGTC 3 cut(s) 167, 419, 623
MmeI TCCRAC 6 cut(s) 396, 447, 621, 768, 804, 1101
Mox20I TGGCCA 1 cut(s) 900
MreI CGCCGGCG 1 cut(s) 601
MroI TCCGGA 1 cut(s) 624
MroNI GCCGGC 1 cut(s) 601
MscI TGGCCA 1 cut(s) 900
MseI TTAA 4 cut(s) 287, 413, 663, 1321
MslI CAYNNNNRTG 1 cut(s) 1169
Msp20I TGGCCA 1 cut(s) 900
MspA1I CMGCKG 2 cut(s) 465, 1070
MspI CCGG 4 cut(s) 282, 602, 625, 650
MspR9I CCNGG 2 cut(s) 317, 650
MvaI CCWGG 1 cut(s) 317
MvnI CGCG 6 cut(s) 186, 465, 753, 913, 1022, 1070
MwoI GCNNNNNNNGC 5 cut(s) 221, 227, 912, 1064, 1245
NaeI GCCGGC 1 cut(s) 603
NarI GGCGCC 1 cut(s) 1248
NciI CCSGG 1 cut(s) 650
NdeII GATC 5 cut(s) 336, 618, 699, 706, 781
NgoMIV GCCGGC 1 cut(s) 601
NlaIII CATG 6 cut(s) 920, 932, 1067, 1168, 1214, 1267
NlaIV GGNNCC 5 cut(s) 469, 507, 508, 882, 1249
NmeAIII GCCGAG 2 cut(s) 616, 883
NmuCI GTSAC 3 cut(s) 307, 529, 1037
NspI RCATGY 1 cut(s) 1067
PaeI GCATGC 1 cut(s) 1067
PagI TCATGA 1 cut(s) 928
PaqCI CACCTGC 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 457
PdiI GCCGGC 1 cut(s) 603
PfeI GAWTC 5 cut(s) 583, 728, 812, 827, 1130
PflFI GACNNNGTC 1 cut(s) 551
PkrI GCNGC 8 cut(s) 185, 223, 573, 600, 973, 1069, 1072, 1247
PleI GAGTC 3 cut(s) 167, 419, 622
PluTI GGCGCC 1 cut(s) 1251
PpsI GAGTC 3 cut(s) 167, 419, 622
PsiI TTATAA 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 315
PspGI CCWGG 1 cut(s) 315
PspN4I GGNNCC 5 cut(s) 469, 507, 508, 882, 1249
PspOMI GGGCCC 1 cut(s) 505
PspPI GGNCC 5 cut(s) 238, 468, 505, 506, 880
PsuI RGATCY 1 cut(s) 336
PsyI GACNNNGTC 1 cut(s) 551
RsaI GTAC 2 cut(s) 132, 1283
RsaNI GTAC 2 cut(s) 131, 1282
RseI CAYNNNNRTG 1 cut(s) 1169
SacII CCGCGG 2 cut(s) 466, 1071
SapI GCTCTTC 1 cut(s) 457
SaqAI TTAA 4 cut(s) 287, 413, 663, 1321
SatI GCNGC 8 cut(s) 184, 222, 572, 599, 972, 1068, 1071, 1246
Sau3AI GATC 5 cut(s) 336, 618, 699, 706, 781
Sau96I GGNCC 5 cut(s) 238, 468, 505, 506, 880
SchI GAGTC 3 cut(s) 167, 419, 623
ScrFI CCNGG 2 cut(s) 317, 650
SduI GDGCHC 3 cut(s) 509, 1107, 1152
SfoI GGCGCC 1 cut(s) 1249
Sfr303I CCGCGG 2 cut(s) 466, 1071
SgrAI CRCCGGYG 1 cut(s) 601
SgrBI CCGCGG 2 cut(s) 466, 1071
SinI GGWCC 3 cut(s) 238, 468, 880
SmiMI CAYNNNNRTG 1 cut(s) 1169
SmlI CTYRAG 2 cut(s) 326, 488
SmoI CTYRAG 2 cut(s) 326, 488
SpeI ACTAGT 1 cut(s) 127
SphI GCATGC 1 cut(s) 1067
Sse9I AATT 3 cut(s) 150, 377, 1001
SspDI GGCGCC 1 cut(s) 1247
SspMI CTAG 4 cut(s) 128, 761, 873, 944
StyD4I CCNGG 2 cut(s) 315, 648
TaaI ACNGT 2 cut(s) 1142, 1195
TaiI ACGT 5 cut(s) 195, 309, 768, 1095, 1227
TaqI TCGA 5 cut(s) 159, 171, 617, 675, 784
TaqII GACCGA 1 cut(s) 650
TasI AATT 3 cut(s) 150, 377, 1001
TatI WGTACW 1 cut(s) 130
TauI GCSGC 7 cut(s) 186, 224, 574, 601, 1070, 1073, 1248
TfiI GAWTC 5 cut(s) 583, 728, 812, 827, 1130
Tru1I TTAA 4 cut(s) 287, 413, 663, 1321
Tru9I TTAA 4 cut(s) 287, 413, 663, 1321
TscAI CASTG 1 cut(s) 443
TseFI GTSAC 3 cut(s) 307, 529, 1037
TseI GCWGC 1 cut(s) 971
Tsp45I GTSAC 3 cut(s) 307, 529, 1037
TspDTI ATGAA 3 cut(s) 43, 917, 1199
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 1 cut(s) 443
Tth111I GACNNNGTC 1 cut(s) 551
VpaK11BI GGWCC 3 cut(s) 238, 468, 880
XapI RAATTY 2 cut(s) 150, 377
XceI RCATGY 1 cut(s) 1067
XspI CTAG 4 cut(s) 128, 761, 873, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.