pycom01g17390

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0016395
Physical Location & Seq
Forward (+)
16696153 .. 16697916
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17390.2

Sequence Viewer

Length: 1224 bp
ATGGATAAAAAACTAGTACAAGTTTCGGAGCAAGAAATTTGTATCGACTTCGCACTCGACTCCAAATGCCGCGCTAACGTGCGCCTTACCTCCCTATGCGCCACCGCCGCCGTAGCCTTCAAGGTCCAAACCTCCTCCCCTCACAAGTTCCTCGTCAAACCGCCCACCGGCTTAATCCCGCCTCTCTCCCACGTCACCTTCCAGGTCATCCTCAAGCCCCAGACCCAGCTCCCGCCCGCCTACCCTCGCTCGCCGTCCGACAAATTCCTCATCCGAACCGCAGAGTTCACCACCGAGTCAACTCACTCCGACTCCATCAACCACTGGTTCTCCAGCTCTTCGTGTCCCCGCGGGTCCACTCAGGATATCAAGCTCAAGGTCGCCTTTGTGGGCCCCTTGCTGTTGCGCCACGCCGTTACTTGTGGGGACTACGACGGCGTCAGGAATATAATCAAGCGGCAGAGGACGATTCTCGCCGAGTTGCCGCCCGCGGAAGCTGAGTCGATCCTCCGGATTGCGACCCAGTTGGATAACCCGGAGAACTTGATTAACTTGCTGCTCGAAGGGGGATTGAGGATCGGCCCGATCGGGGTAAGTGTGAGCAGCGATCAAGTGCATTACGAAGAAGATTCTAAATATCCATCCAACGGACGCGATGAGCTAGACGTGGCAGAGGCGTGTGATCGACTGGATGATGTTCTGGGACTGAGGGAATCTGAGCCGTTGGATTCTAAGGACAAGGGAAATGGCAGGCGTGCCGAGGTGAGGAAATCTAGTGCGGACCCCACGACAACTATTGGTCACAGCCGTTCGCGCCATGATTTTGCTCATGACAAAGATAAACTAGAAATGGGAGAACTAATGTTGATGGCAGCGAGAAGAGGAGATTTGAAGAATGTTGAATTGTTGTTACAAAATGGCGCGGATATAAACTGCTGTGATCAATATGGTTTGACAGCCCTGCATGCCGCGGCCATCAAGGGCCACAAGGACGTTGCTCGGGTGCTCATCCGATTTGGGTCGGATTTGGAATCCCAAGACGGTGAAGGCCACGCACCCTTGCATATGGCAGTCGTCGGCGGGAGCTTAGAGACGGTTAAGATCTTGGTTCATAGTGGGGCTAACGTCAATGCCAAGAGCAACGGCGGCGCCACCCCTCTGTACATGGCTACTGCTATGGGGTACGATGATATAACTGAGTTTCTTACCAGCAGAAAACATTAA

Protein Analysis

408

Amino Acids

44.42

Weight (kDa)

6.21

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016395)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24970 FvH4_7g24970
malus_domestica MD01G1157100.v1.1
prunus_persica Prupe.2G254900_v2.0.a1
pyrus_communis pycom01g17390
rosa_chinensis RchiOBHm_Chr1g0370871
rosa_laevigata RLG00000027003
rosa_multiflora Rmu_sc0009474.1_g000017
rosa_roxburghii Rroxscaffold_4G00286170
rosa_rugosa Rorug01G0360000
rosa_samantha Rh1AG369700 Rh1BG333300 Rh1CG346700 Rh1DG364500
rosa_wichuraiana Rw1G032550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1148
AccII CGCG 7 cut(s) 72, 351, 491, 654, 814, 923, 971
AccIII TCCGGA 1 cut(s) 510
AclWI GGATC 2 cut(s) 499, 584
AcoI YGGCCR 1 cut(s) 972
AcsI RAATTY 2 cut(s) 36, 263
AcyI GRCGYC 2 cut(s) 438, 1149
AfaI GTAC 3 cut(s) 18, 1163, 1184
AfiI CCNNNNNNNGG 4 cut(s) 167, 589, 647, 765
AgsI TTSAA 3 cut(s) 121, 892, 902
AhlI ACTAGT 1 cut(s) 13
AjiI CACGTC 2 cut(s) 193, 667
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 6 cut(s) 229, 336, 373, 497, 661, 1086
AluI AGCT 6 cut(s) 229, 336, 373, 497, 661, 1086
Alw21I GWGCWC 1 cut(s) 1008
Alw26I GTCTC 1 cut(s) 1085
AlwI GGATC 2 cut(s) 499, 584
Ama87I CYCGRG 1 cut(s) 999
Aor13HI TCCGGA 1 cut(s) 510
AoxI GGCC 5 cut(s) 391, 580, 972, 982, 1048
ApaI GGGCCC 1 cut(s) 395
ApeKI GCWGC 3 cut(s) 556, 603, 872
ApoI RAATTY 2 cut(s) 36, 263
AspLEI GCGC 7 cut(s) 74, 84, 101, 408, 816, 923, 1151
AspS9I GGNCC 7 cut(s) 124, 354, 391, 392, 581, 781, 982
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 4 cut(s) 187, 280, 775, 1055
AvaI CYCGRG 1 cut(s) 999
AvaII GGWCC 3 cut(s) 124, 354, 781
BaeGI GKGCMC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 1148
BanII GRGCYC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 1008
BbvI GCAGC 3 cut(s) 543, 615, 884
BccI CCATC 4 cut(s) 323, 649, 862, 983
BceAI ACGGC 7 cut(s) 95, 238, 398, 451, 706, 792, 1159
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 940
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 1 cut(s) 1085
BcuI ACTAGT 1 cut(s) 13
BfaI CTAG 4 cut(s) 14, 662, 774, 845
BfoI RGCGCY 1 cut(s) 1152
BglII AGATCT 1 cut(s) 1101
Bme1390I CCNGG 2 cut(s) 203, 536
Bme18I GGWCC 3 cut(s) 124, 354, 781
BmeT110I CYCGRG 1 cut(s) 999
BmgBI CACGTC 2 cut(s) 193, 667
BmgT120I GGNCC 7 cut(s) 124, 354, 391, 392, 581, 781, 982
BmiI GGNNCC 5 cut(s) 355, 393, 394, 783, 1150
BmrFI CCNGG 2 cut(s) 203, 536
BmrI ACTGGG 1 cut(s) 517
BmuI ACTGGG 1 cut(s) 517
BpmI CTGGAG 1 cut(s) 316
BpuEI CTTGAG 2 cut(s) 197, 359
BpuMI CCSGG 1 cut(s) 536
BsaHI GRCGYC 2 cut(s) 438, 1149
BsaJI CCNNGG 4 cut(s) 349, 489, 759, 969
BsaWI WCCGGW 1 cut(s) 510
BsaXI ACNNNNNCTCC 4 cut(s) 296, 314, 326, 344
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 589, 647, 765
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 3 cut(s) 329, 523, 693
BseAI TCCGGA 1 cut(s) 510
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 4 cut(s) 349, 489, 759, 969
BseGI GGATG 5 cut(s) 207, 270, 641, 697, 1008
BseLI CCNNNNNNNGG 4 cut(s) 167, 589, 647, 765
BseMII CTCAG 5 cut(s) 374, 489, 698, 708, 1188
BseNI ACTGG 3 cut(s) 329, 523, 693
BseRI GAGGAG 2 cut(s) 124, 897
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 3 cut(s) 543, 615, 884
BseYI CCCAGC 1 cut(s) 225
Bsh1236I CGCG 7 cut(s) 72, 351, 491, 654, 814, 923, 971
Bsh1285I CGRYCG 1 cut(s) 588
BshFI GGCC 5 cut(s) 393, 582, 974, 984, 1050
BshNI GGYRCC 1 cut(s) 1148
BsiEI CGRYCG 1 cut(s) 588
BsiHKAI GWGCWC 1 cut(s) 1008
BsiHKCI CYCGRG 1 cut(s) 999
BsiSI CCGG 3 cut(s) 168, 511, 536
BslFI GGGAC 3 cut(s) 330, 440, 717
BslI CCNNNNNNNGG 4 cut(s) 167, 589, 647, 765
BsmAI GTCTC 1 cut(s) 1085
BsmBI CGTCTC 1 cut(s) 1085
BsmFI GGGAC 3 cut(s) 330, 440, 717
BsnI GGCC 5 cut(s) 393, 582, 974, 984, 1050
BsoBI CYCGRG 1 cut(s) 999
Bsp120I GGGCCC 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 395, 1008
Bsp13I TCCGGA 1 cut(s) 510
Bsp1407I TGTACA 1 cut(s) 1161
Bsp143I GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
BspANI GGCC 5 cut(s) 393, 582, 974, 984, 1050
BspCNI CTCAG 5 cut(s) 373, 490, 699, 709, 1189
BspEI TCCGGA 1 cut(s) 510
BspFNI CGCG 7 cut(s) 72, 351, 491, 654, 814, 923, 971
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 5 cut(s) 355, 393, 394, 783, 1150
BspPI GGATC 2 cut(s) 499, 584
BspQI GCTCTTC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 1148
BsrFI RCCGGY 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 3 cut(s) 329, 523, 693
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 349, 489, 759, 969
BssMI GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
BssNI GRCGYC 2 cut(s) 438, 1149
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 1043, 1096
Bst6I CTCTTC 2 cut(s) 343, 874
BstACI GRCGYC 2 cut(s) 438, 1149
BstAUI TGTACA 1 cut(s) 1161
BstC8I GCNNGC 6 cut(s) 237, 251, 489, 752, 756, 966
BstDEI CTNAG 7 cut(s) 360, 498, 707, 717, 732, 1087, 1197
BstDSI CCRYGG 3 cut(s) 349, 489, 969
BstF5I GGATG 5 cut(s) 207, 270, 641, 697, 1008
BstFNI CGCG 7 cut(s) 72, 351, 491, 654, 814, 923, 971
BstH2I RGCGCY 1 cut(s) 1152
BstHHI GCGC 7 cut(s) 74, 84, 101, 408, 816, 923, 1151
BstKTI GATC 7 cut(s) 507, 579, 588, 610, 685, 943, 1104
BstMAI GTCTC 1 cut(s) 1085
BstMBI GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
BstMCI CGRYCG 1 cut(s) 588
BstMWI GCNNNNNNNGC 5 cut(s) 107, 113, 813, 965, 1146
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 968
BstSCI CCNGG 2 cut(s) 201, 534
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 7 cut(s) 72, 351, 491, 654, 814, 923, 971
BstV1I GCAGC 3 cut(s) 543, 615, 884
BstX2I RGATCY 1 cut(s) 1101
BstYI RGATCY 1 cut(s) 1101
BsuRI GGCC 5 cut(s) 393, 582, 974, 984, 1050
BtgI CCRYGG 3 cut(s) 349, 489, 969
BtgZI GCGATG 1 cut(s) 669
BtrI CACGTC 2 cut(s) 193, 667
BtsCI GGATG 5 cut(s) 207, 270, 641, 697, 1008
BtsIMutI CAGTG 1 cut(s) 322
Cac8I GCNNGC 6 cut(s) 237, 251, 489, 752, 756, 966
CciI TCATGA 1 cut(s) 829
CfoI GCGC 7 cut(s) 74, 84, 101, 408, 816, 923, 1151
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 7 cut(s) 124, 354, 391, 392, 581, 781, 982
Cfr42I CCGCGG 3 cut(s) 352, 492, 972
CseI GACGC 2 cut(s) 427, 660
Csp6I GTAC 3 cut(s) 17, 1162, 1183
CviAII CATG 4 cut(s) 818, 830, 965, 1165
CviQI GTAC 3 cut(s) 17, 1162, 1183
DdeI CTNAG 7 cut(s) 360, 498, 707, 717, 732, 1087, 1197
DinI GGCGCC 1 cut(s) 1150
DpnI GATC 7 cut(s) 506, 578, 587, 609, 684, 942, 1103
DpnII GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
EaeI YGGCCR 1 cut(s) 972
Eam1104I CTCTTC 2 cut(s) 343, 874
EarI CTCTTC 2 cut(s) 343, 874
Eco24I GRGCYC 1 cut(s) 395
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 3 cut(s) 124, 354, 781
Eco88I CYCGRG 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 395
EgeI GGCGCC 1 cut(s) 1150
EheI GGCGCC 1 cut(s) 1150
Esp3I CGTCTC 1 cut(s) 1085
FaeI CATG 4 cut(s) 821, 833, 968, 1168
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 3 cut(s) 330, 440, 717
FatI CATG 4 cut(s) 817, 829, 964, 1164
FauI CCCGC 7 cut(s) 186, 240, 244, 344, 356, 496, 1073
FauNDI CATATG 1 cut(s) 1065
FbaI TGATCA 1 cut(s) 940
FokI GGATG 5 cut(s) 194, 257, 628, 704, 995
FriOI GRGCYC 1 cut(s) 395
FspBI CTAG 4 cut(s) 14, 662, 774, 845
GlaI GCGC 7 cut(s) 73, 83, 100, 407, 815, 922, 1150
GsaI CCCAGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 1152
HaeIII GGCC 5 cut(s) 393, 582, 974, 984, 1050
HapII CCGG 3 cut(s) 168, 511, 536
HgaI GACGC 2 cut(s) 427, 660
HhaI GCGC 7 cut(s) 74, 84, 101, 408, 816, 923, 1151
Hin1I GRCGYC 2 cut(s) 438, 1149
Hin1II CATG 4 cut(s) 821, 833, 968, 1168
Hin6I GCGC 7 cut(s) 72, 82, 99, 406, 814, 921, 1149
HinP1I GCGC 7 cut(s) 72, 82, 99, 406, 814, 921, 1149
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 9 cut(s) 59, 296, 311, 469, 500, 629, 713, 728, 1031
HpaII CCGG 3 cut(s) 168, 511, 536
HphI GGTGA 4 cut(s) 187, 280, 775, 1055
Hpy166II GTNNAC 3 cut(s) 288, 300, 357
Hpy188I TCNGA 7 cut(s) 28, 259, 275, 310, 718, 1013, 1024
Hpy188III TCNNGA 4 cut(s) 362, 442, 511, 830
Hpy8I GTNNAC 3 cut(s) 288, 300, 357
Hpy99I CGWCG 2 cut(s) 437, 1079
HpyAV CCTTC 4 cut(s) 127, 208, 557, 1040
HpyCH4III ACNGT 2 cut(s) 1043, 1096
HpyCH4IV ACGT 5 cut(s) 78, 192, 666, 993, 1125
HpyCH4V TGCA 3 cut(s) 616, 964, 1063
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 113, 813, 965, 1146
HpyF3I CTNAG 7 cut(s) 360, 498, 707, 717, 732, 1087, 1197
HpySE526I ACGT 5 cut(s) 78, 192, 666, 993, 1125
Hsp92I GRCGYC 2 cut(s) 438, 1149
Hsp92II CATG 4 cut(s) 821, 833, 968, 1168
HspAI GCGC 7 cut(s) 72, 82, 99, 406, 814, 921, 1149
KasI GGCGCC 1 cut(s) 1148
Kpn2I TCCGGA 1 cut(s) 510
Ksp22I TGATCA 1 cut(s) 940
KspI CCGCGG 3 cut(s) 352, 492, 972
Kzo9I GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
LguI GCTCTTC 1 cut(s) 343
LmnI GCTCC 3 cut(s) 28, 234, 1083
Lsp1109I GCAGC 3 cut(s) 543, 615, 884
MaeI CTAG 4 cut(s) 14, 662, 774, 845
MaeII ACGT 5 cut(s) 78, 192, 666, 993, 1125
MaeIII GTNAC 4 cut(s) 193, 415, 800, 909
MalI GATC 7 cut(s) 506, 578, 587, 609, 684, 942, 1103
MboI GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
MboII GAAGA 5 cut(s) 330, 635, 638, 891, 904
MflI RGATCY 1 cut(s) 1101
MhlI GDGCHC 2 cut(s) 395, 1008
MluCI AATT 3 cut(s) 36, 263, 902
Mly113I GGCGCC 1 cut(s) 1149
MlyI GAGTC 4 cut(s) 53, 305, 305, 509
MmeI TCCRAC 6 cut(s) 282, 333, 507, 669, 705, 1002
MroI TCCGGA 1 cut(s) 510
MseI TTAA 4 cut(s) 173, 549, 1098, 1222
MspA1I CMGCKG 3 cut(s) 351, 491, 971
MspI CCGG 3 cut(s) 168, 511, 536
MspR9I CCNGG 2 cut(s) 203, 536
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 7 cut(s) 72, 351, 491, 654, 814, 923, 971
MwoI GCNNNNNNNGC 5 cut(s) 107, 113, 813, 965, 1146
NarI GGCGCC 1 cut(s) 1149
NciI CCSGG 1 cut(s) 536
NdeI CATATG 1 cut(s) 1065
NdeII GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
NlaIII CATG 4 cut(s) 821, 833, 968, 1168
NlaIV GGNNCC 5 cut(s) 355, 393, 394, 783, 1150
NmeAIII GCCGAG 2 cut(s) 502, 784
NmuCI GTSAC 2 cut(s) 193, 800
NspI RCATGY 1 cut(s) 968
PaeI GCATGC 1 cut(s) 968
PagI TCATGA 1 cut(s) 829
PciSI GCTCTTC 1 cut(s) 343
PfeI GAWTC 5 cut(s) 469, 629, 713, 728, 1031
PflFI GACNNNGTC 1 cut(s) 437
Ple19I CGATCG 1 cut(s) 588
PleI GAGTC 4 cut(s) 53, 304, 305, 508
PluTI GGCGCC 1 cut(s) 1152
PpsI GAGTC 4 cut(s) 53, 304, 305, 508
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 5 cut(s) 355, 393, 394, 783, 1150
PspOMI GGGCCC 1 cut(s) 391
PspPI GGNCC 7 cut(s) 124, 354, 391, 392, 581, 781, 982
PsuI RGATCY 1 cut(s) 1101
PsyI GACNNNGTC 1 cut(s) 437
PvuI CGATCG 1 cut(s) 588
RsaI GTAC 3 cut(s) 18, 1163, 1184
RsaNI GTAC 3 cut(s) 17, 1162, 1183
SacII CCGCGG 3 cut(s) 352, 492, 972
SapI GCTCTTC 1 cut(s) 343
SaqAI TTAA 4 cut(s) 173, 549, 1098, 1222
Sau3AI GATC 7 cut(s) 504, 576, 585, 607, 682, 940, 1101
Sau96I GGNCC 7 cut(s) 124, 354, 391, 392, 581, 781, 982
SchI GAGTC 4 cut(s) 53, 305, 305, 509
ScrFI CCNGG 2 cut(s) 203, 536
SduI GDGCHC 2 cut(s) 395, 1008
SfoI GGCGCC 1 cut(s) 1150
Sfr303I CCGCGG 3 cut(s) 352, 492, 972
SgrBI CCGCGG 3 cut(s) 352, 492, 972
SinI GGWCC 3 cut(s) 124, 354, 781
SmlI CTYRAG 2 cut(s) 212, 374
SmoI CTYRAG 2 cut(s) 212, 374
SpeI ACTAGT 1 cut(s) 13
SphI GCATGC 1 cut(s) 968
Sse9I AATT 3 cut(s) 36, 263, 902
SspDI GGCGCC 1 cut(s) 1148
SspMI CTAG 4 cut(s) 14, 662, 774, 845
StyD4I CCNGG 2 cut(s) 201, 534
TaaI ACNGT 2 cut(s) 1043, 1096
TaiI ACGT 5 cut(s) 81, 195, 669, 996, 1128
TaqI TCGA 5 cut(s) 45, 57, 503, 561, 685
TasI AATT 3 cut(s) 36, 263, 902
TatI WGTACW 2 cut(s) 16, 1161
TauI GCSGC 7 cut(s) 72, 110, 460, 487, 971, 974, 1149
TfiI GAWTC 5 cut(s) 469, 629, 713, 728, 1031
Tru1I TTAA 4 cut(s) 173, 549, 1098, 1222
Tru9I TTAA 4 cut(s) 173, 549, 1098, 1222
TscAI CASTG 1 cut(s) 329
TseFI GTSAC 2 cut(s) 193, 800
TseI GCWGC 3 cut(s) 556, 603, 872
Tsp45I GTSAC 2 cut(s) 193, 800
TspDTI ATGAA 1 cut(s) 1100
TspGWI ACGGA 1 cut(s) 663
TspRI CASTG 1 cut(s) 329
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 3 cut(s) 124, 354, 781
XapI RAATTY 2 cut(s) 36, 263
XceI RCATGY 1 cut(s) 968
XspI CTAG 4 cut(s) 14, 662, 774, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.