Rw1G032550

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016395
Physical Location & Seq
Forward (+)
60768769 .. 60770479
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032550.1

Sequence Viewer

Length: 1089 bp
ATGGAGAATTCGAAACTGGTCCAAGTCTCGGAGCAAGAGGTCCGAATCGACTTCACCTTAAACCAAAAATGCCGCGCCAACGTCCGCCTGACCTCCCTGAGCTCCACGTGCCCCGTCGCCTTCAAGGTCCAGACCTCCTCCCCTCACAAGTTCCTCGTCAAGCCCCCCACCGGCCTCATCCCTCCCTTATCCTTCGCCACCTTCCAGGTCGTCCTCAAGCCCCAACCGCCGAGTTCTTCCCCCTCCGCCGGCAACTCCGACTCCCTCGACTCCTGGTTCAGAGGGGCCCACACTCGCGACCTCAAGCTCAAGGTCGCCTTCGTGGGCCCCGTTCTTCTGCGGCACGCCGTGCACTGCGGGGATTGCGAGGCGGTCAGGAATATAATCAGGCGGCAGAGGACGATTCTGGCGGAGCTGACGCCAGGGGAGGCCGAGTCGCTGCTCCGAGTCGCGACCGAGTTGGCCGAGCCGGAGAGGATGATTAATTTGCTGCTTGAGGCCGGGTTGAGGATCGAGGCGGGTCTGAGGTTGGATCATGTGGATGAGGAATTGGTGCGGAACGGGATGGATGAGCTACGCGTGGCGGAGGCTTCTGATCGGTTTGCTAGTGTTTTGGGTTTGGGGGAATCGGGACCGTCGACAGCAGAAGACCCCAGGATAACTAAAGGTCATGGCCATCGTCCGCGACTTGCTACTGATAGTGATAAGCTAGAATTGGGGGAACAAATGTTGATGGCAGCGCGAATCGGGGATTTGAAAGATGTGGAACTGTTGTTTCTTGAAGGAGCAGACATTAGCTACTGTGATCAATATGGCTTGACAGCTCTTCATGCAGCAGCCATTAAAGGGCACAAGGAGGTTGTTCTGATGCTGGTCGAATTTGGGCTAGACTTGGAATCCAGAGACAGTGAAGGCCATGCGCCGCTGCATTTGGCGGTTGTGGGGGGCAACTTGGAGACGGTTGAGATGTTGATACAGAATGGGGCTAATGTGAATGCCAAGAGCAAGAGTGGGGCTACTCCTTTGTACATGGCCACAGCTATGGGGTATGATGATATCACTGAGTTTCTTATCAGCAGAGGAGGCTGA

Protein Analysis

362

Amino Acids

39.33

Weight (kDa)

5.53

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 17 - 75 8.6e-09 MSP (Major sperm protein) domain
Ank_2 PF12796 245 - 334 6.2e-20 Ankyrin repeats (3 copies)
Ank_4 PF13637 247 - 292 4.7e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 266 - 312 5.4e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 276 - 325 3.4e-09 Ankyrin repeats (many copies)
Ank PF00023 305 - 335 6.9e-08 Ankyrin repeat
Ank_4 PF13637 309 - 358 1e-09 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016395)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24970 FvH4_7g24970
malus_domestica MD01G1157100.v1.1
prunus_persica Prupe.2G254900_v2.0.a1
pyrus_communis pycom01g17390
rosa_chinensis RchiOBHm_Chr1g0370871
rosa_laevigata RLG00000027003
rosa_multiflora Rmu_sc0009474.1_g000017
rosa_roxburghii Rroxscaffold_4G00286170
rosa_rugosa Rorug01G0360000
rosa_samantha Rh1AG369700 Rh1BG333300 Rh1CG346700 Rh1DG364500
rosa_wichuraiana Rw1G032550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 638
AccII CGCG 6 cut(s) 75, 297, 452, 579, 685, 742
AclWI GGATC 2 cut(s) 518, 540
AcoI YGGCCR 3 cut(s) 462, 673, 1032
AcsI RAATTY 2 cut(s) 7, 878
AcvI CACGTG 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 419
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 1 cut(s) 1028
AfiI CCNNNNNNNGG 5 cut(s) 28, 170, 248, 507, 846
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 3 cut(s) 124, 757, 782
AjnI CCWGG 4 cut(s) 204, 272, 421, 653
AluBI AGCT 8 cut(s) 102, 307, 415, 574, 709, 798, 824, 1040
AluI AGCT 8 cut(s) 102, 307, 415, 574, 709, 798, 824, 1040
Alw21I GWGCWC 2 cut(s) 104, 354
Alw26I GTCTC 3 cut(s) 31, 897, 950
Alw44I GTGCAC 1 cut(s) 350
AlwI GGATC 2 cut(s) 518, 540
AoxI GGCC 9 cut(s) 172, 285, 325, 429, 462, 498, 673, 913, 1032
ApaI GGGCCC 2 cut(s) 289, 329
ApaLI GTGCAC 1 cut(s) 350
ApeKI GCWGC 6 cut(s) 439, 490, 737, 833, 836, 925
ApoI RAATTY 2 cut(s) 7, 878
AseI ATTAAT 1 cut(s) 483
AspLEI GCGC 3 cut(s) 77, 742, 922
AspS9I GGNCC 8 cut(s) 19, 40, 127, 285, 286, 325, 326, 632
AsuC2I CCSGG 1 cut(s) 502
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 11
AvaII GGWCC 4 cut(s) 19, 40, 127, 632
BaeGI GKGCMC 5 cut(s) 113, 289, 329, 354, 852
BalI TGGCCA 2 cut(s) 675, 1034
BanII GRGCYC 3 cut(s) 104, 289, 329
BbrPI CACGTG 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 2 cut(s) 104, 354
BbvI GCAGC 6 cut(s) 426, 477, 749, 845, 848, 912
BccI CCATC 3 cut(s) 559, 684, 727
BceAI ACGGC 1 cut(s) 332
BciT130I CCWGG 4 cut(s) 206, 274, 423, 655
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 1 cut(s) 502
BcoDI GTCTC 3 cut(s) 31, 897, 950
BfaI CTAG 3 cut(s) 606, 710, 887
Bme1390I CCNGG 5 cut(s) 206, 274, 423, 502, 655
Bme18I GGWCC 4 cut(s) 19, 40, 127, 632
BmgT120I GGNCC 8 cut(s) 19, 40, 127, 285, 286, 325, 326, 632
BmiI GGNNCC 5 cut(s) 286, 287, 327, 328, 633
BmrFI CCNGG 5 cut(s) 206, 274, 423, 502, 655
BmsI GCATC 1 cut(s) 858
BpiI GAAGAC 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 98
Bpu14I TTCGAA 1 cut(s) 11
BpuEI CTTGAG 4 cut(s) 200, 287, 293, 515
BpuMI CCSGG 1 cut(s) 502
BsaAI YACGTR 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 422, 653
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 419, 449
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 170, 248, 507, 846
Bse118I RCCGGY 2 cut(s) 170, 248
Bse1I ACTGG 1 cut(s) 21
BseBI CCWGG 4 cut(s) 206, 274, 423, 655
BseDI CCNNGG 2 cut(s) 422, 653
BseGI GGATG 5 cut(s) 177, 483, 547, 570, 574
BseLI CCNNNNNNNGG 5 cut(s) 28, 170, 248, 507, 846
BseMII CTCAG 3 cut(s) 89, 515, 1053
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 127
BseSI GKGCMC 5 cut(s) 113, 289, 329, 354, 852
BseXI GCAGC 6 cut(s) 426, 477, 749, 845, 848, 912
Bsh1236I CGCG 6 cut(s) 75, 297, 452, 579, 685, 742
Bsh1285I CGRYCG 1 cut(s) 456
BshFI GGCC 9 cut(s) 174, 287, 327, 431, 464, 500, 675, 915, 1034
BsiEI CGRYCG 1 cut(s) 456
BsiHKAI GWGCWC 2 cut(s) 104, 354
BsiSI CCGG 4 cut(s) 171, 249, 470, 501
BslFI GGGAC 1 cut(s) 645
BslI CCNNNNNNNGG 5 cut(s) 28, 170, 248, 507, 846
BsmAI GTCTC 3 cut(s) 31, 897, 950
BsmBI CGTCTC 1 cut(s) 950
BsmFI GGGAC 1 cut(s) 645
BsmI GAATGC 1 cut(s) 1000
BsnI GGCC 9 cut(s) 174, 287, 327, 431, 464, 500, 675, 915, 1034
Bsp119I TTCGAA 1 cut(s) 11
Bsp120I GGGCCC 2 cut(s) 285, 325
Bsp1286I GDGCHC 6 cut(s) 104, 113, 289, 329, 354, 852
Bsp1407I TGTACA 1 cut(s) 1026
Bsp143I GATC 4 cut(s) 510, 532, 595, 805
Bsp68I TCGCGA 2 cut(s) 297, 452
BspANI GGCC 9 cut(s) 174, 287, 327, 431, 464, 500, 675, 915, 1034
BspCNI CTCAG 3 cut(s) 90, 516, 1054
BspFNI CGCG 6 cut(s) 75, 297, 452, 579, 685, 742
BspLI GGNNCC 5 cut(s) 286, 287, 327, 328, 633
BspPI GGATC 2 cut(s) 518, 540
BspQI GCTCTTC 1 cut(s) 831
BspT104I TTCGAA 1 cut(s) 11
BsrFI RCCGGY 2 cut(s) 170, 248
BsrGI TGTACA 1 cut(s) 1026
BsrI ACTGG 1 cut(s) 21
BssAI RCCGGY 2 cut(s) 170, 248
BssECI CCNNGG 2 cut(s) 422, 653
BssMI GATC 4 cut(s) 510, 532, 595, 805
BssNI GRCGYC 1 cut(s) 419
Bst2UI CCWGG 4 cut(s) 206, 274, 423, 655
Bst4CI ACNGT 5 cut(s) 636, 771, 803, 908, 961
Bst6I CTCTTC 1 cut(s) 831
BstACI GRCGYC 1 cut(s) 419
BstAPI GCANNNNNTGC 1 cut(s) 349
BstAUI TGTACA 1 cut(s) 1026
BstBAI YACGTR 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 250, 345
BstDEI CTNAG 3 cut(s) 98, 524, 1062
BstF5I GGATG 5 cut(s) 177, 483, 547, 570, 574
BstFNI CGCG 6 cut(s) 75, 297, 452, 579, 685, 742
BstHHI GCGC 3 cut(s) 77, 742, 922
BstKTI GATC 4 cut(s) 513, 535, 598, 808
BstMAI GTCTC 3 cut(s) 31, 897, 950
BstMBI GATC 4 cut(s) 510, 532, 595, 805
BstMCI CGRYCG 1 cut(s) 456
BstMWI GCNNNNNNNGC 6 cut(s) 108, 226, 349, 363, 830, 1083
BstNI CCWGG 4 cut(s) 206, 274, 423, 655
BstSCI CCNGG 5 cut(s) 204, 272, 421, 500, 653
BstSLI GKGCMC 5 cut(s) 113, 289, 329, 354, 852
BstUI CGCG 6 cut(s) 75, 297, 452, 579, 685, 742
BstV1I GCAGC 6 cut(s) 426, 477, 749, 845, 848, 912
BstV2I GAAGAC 1 cut(s) 654
BstXI CCANNNNNNTGG 1 cut(s) 1042
BsuRI GGCC 9 cut(s) 174, 287, 327, 431, 464, 500, 675, 915, 1034
BtsCI GGATG 5 cut(s) 177, 483, 547, 570, 574
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 3 cut(s) 352, 913, 1059
BtuMI TCGCGA 2 cut(s) 297, 452
Cac8I GCNNGC 2 cut(s) 250, 345
CfoI GCGC 3 cut(s) 77, 742, 922
Cfr10I RCCGGY 2 cut(s) 170, 248
Cfr13I GGNCC 8 cut(s) 19, 40, 127, 285, 286, 325, 326, 632
CseI GACGC 1 cut(s) 427
Csp6I GTAC 1 cut(s) 1027
CviAII CATG 5 cut(s) 536, 671, 830, 917, 1030
CviQI GTAC 1 cut(s) 1027
DdeI CTNAG 3 cut(s) 98, 524, 1062
DpnI GATC 4 cut(s) 512, 534, 597, 807
DpnII GATC 4 cut(s) 510, 532, 595, 805
DraIII CACNNNGTG 1 cut(s) 349
EaeI YGGCCR 3 cut(s) 462, 673, 1032
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
EciI GGCGGA 4 cut(s) 74, 235, 425, 599
Ecl136II GAGCTC 1 cut(s) 102
Eco24I GRGCYC 3 cut(s) 104, 289, 329
Eco32I GATATC 1 cut(s) 1057
Eco47I GGWCC 4 cut(s) 19, 40, 127, 632
Eco53kI GAGCTC 1 cut(s) 102
Eco72I CACGTG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 102
EcoO109I RGGNCCY 2 cut(s) 285, 326
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 4 cut(s) 204, 272, 421, 653
EcoRV GATATC 1 cut(s) 1057
EcoT38I GRGCYC 3 cut(s) 104, 289, 329
Esp3I CGTCTC 1 cut(s) 950
FaeI CATG 5 cut(s) 539, 674, 833, 920, 1033
FaiI YATR 9 cut(s) 383, 537, 672, 813, 831, 918, 1031, 1043, 1050
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 1 cut(s) 645
FatI CATG 5 cut(s) 535, 670, 829, 916, 1029
FauI CCCGC 2 cut(s) 350, 511
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 1 cut(s) 638
FokI GGATG 5 cut(s) 164, 490, 554, 577, 581
FriOI GRGCYC 3 cut(s) 104, 289, 329
FspBI CTAG 3 cut(s) 606, 710, 887
GlaI GCGC 3 cut(s) 76, 741, 921
HaeIII GGCC 9 cut(s) 174, 287, 327, 431, 464, 500, 675, 915, 1034
HapII CCGG 4 cut(s) 171, 249, 470, 501
HgaI GACGC 1 cut(s) 427
HhaI GCGC 3 cut(s) 77, 742, 922
Hin1I GRCGYC 1 cut(s) 419
Hin1II CATG 5 cut(s) 539, 674, 833, 920, 1033
Hin6I GCGC 3 cut(s) 75, 740, 920
HinP1I GCGC 3 cut(s) 75, 740, 920
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HinfI GANTC 9 cut(s) 45, 260, 269, 403, 434, 447, 626, 744, 896
HpaII CCGG 4 cut(s) 171, 249, 470, 501
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 352, 639
Hpy188I TCNGA 8 cut(s) 31, 44, 259, 281, 446, 525, 595, 867
Hpy188III TCNNGA 7 cut(s) 130, 296, 376, 451, 630, 779, 900
Hpy8I GTNNAC 2 cut(s) 352, 639
Hpy99I CGWCG 2 cut(s) 119, 640
HpyAV CCTTC 6 cut(s) 130, 202, 211, 328, 776, 905
HpyCH4III ACNGT 5 cut(s) 636, 771, 803, 908, 961
HpyCH4IV ACGT 2 cut(s) 81, 107
HpyCH4V TGCA 3 cut(s) 352, 833, 928
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 226, 349, 363, 830, 1083
HpyF3I CTNAG 3 cut(s) 98, 524, 1062
HpySE526I ACGT 2 cut(s) 81, 107
Hsp92I GRCGYC 1 cut(s) 419
Hsp92II CATG 5 cut(s) 539, 674, 833, 920, 1033
HspAI GCGC 3 cut(s) 75, 740, 920
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 4 cut(s) 510, 532, 595, 805
LguI GCTCTTC 1 cut(s) 831
LmnI GCTCC 5 cut(s) 31, 107, 412, 447, 785
Lsp1109I GCAGC 6 cut(s) 426, 477, 749, 845, 848, 912
LweI GCATC 1 cut(s) 858
MaeI CTAG 3 cut(s) 606, 710, 887
MaeII ACGT 2 cut(s) 81, 107
MalI GATC 4 cut(s) 512, 534, 597, 807
MboI GATC 4 cut(s) 510, 532, 595, 805
MboII GAAGA 4 cut(s) 228, 326, 659, 818
MhlI GDGCHC 6 cut(s) 104, 113, 289, 329, 354, 852
MlsI TGGCCA 2 cut(s) 675, 1034
MluCI AATT 5 cut(s) 7, 484, 548, 713, 878
MluI ACGCGT 1 cut(s) 577
MluNI TGGCCA 2 cut(s) 675, 1034
MlyI GAGTC 4 cut(s) 254, 263, 443, 456
MmeI TCCRAC 2 cut(s) 282, 510
Mox20I TGGCCA 2 cut(s) 675, 1034
MroNI GCCGGC 1 cut(s) 248
MscI TGGCCA 2 cut(s) 675, 1034
MseI TTAA 3 cut(s) 59, 483, 843
MslI CAYNNNNRTG 2 cut(s) 540, 1040
Msp20I TGGCCA 2 cut(s) 675, 1034
MspA1I CMGCKG 1 cut(s) 925
MspI CCGG 4 cut(s) 171, 249, 470, 501
MspR9I CCNGG 5 cut(s) 206, 274, 423, 502, 655
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 4 cut(s) 206, 274, 423, 655
MvnI CGCG 6 cut(s) 75, 297, 452, 579, 685, 742
MwoI GCNNNNNNNGC 6 cut(s) 108, 226, 349, 363, 830, 1083
NaeI GCCGGC 1 cut(s) 250
NciI CCSGG 1 cut(s) 502
NdeII GATC 4 cut(s) 510, 532, 595, 805
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 5 cut(s) 539, 674, 833, 920, 1033
NlaIV GGNNCC 5 cut(s) 286, 287, 327, 328, 633
NmeAIII GCCGAG 3 cut(s) 255, 457, 490
NruI TCGCGA 2 cut(s) 297, 452
NspV TTCGAA 1 cut(s) 11
PciSI GCTCTTC 1 cut(s) 831
PcsI WCGNNNNNNNCGW 2 cut(s) 327, 635
PctI GAATGC 1 cut(s) 1000
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 5 cut(s) 45, 403, 626, 744, 896
PleI GAGTC 4 cut(s) 254, 263, 442, 455
PmaCI CACGTG 1 cut(s) 108
PmlI CACGTG 1 cut(s) 108
PpsI GAGTC 4 cut(s) 254, 263, 442, 455
Ppu21I YACGTR 1 cut(s) 108
PshBI ATTAAT 1 cut(s) 483
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 4 cut(s) 204, 272, 421, 653
PspCI CACGTG 1 cut(s) 108
PspGI CCWGG 4 cut(s) 204, 272, 421, 653
PspN4I GGNNCC 5 cut(s) 286, 287, 327, 328, 633
PspOMI GGGCCC 2 cut(s) 285, 325
PspPI GGNCC 8 cut(s) 19, 40, 127, 285, 286, 325, 326, 632
RruI TCGCGA 2 cut(s) 297, 452
RsaI GTAC 1 cut(s) 1028
RsaNI GTAC 1 cut(s) 1027
RseI CAYNNNNRTG 2 cut(s) 540, 1040
SacI GAGCTC 1 cut(s) 104
SalI GTCGAC 1 cut(s) 637
SapI GCTCTTC 1 cut(s) 831
SaqAI TTAA 3 cut(s) 59, 483, 843
Sau3AI GATC 4 cut(s) 510, 532, 595, 805
Sau96I GGNCC 8 cut(s) 19, 40, 127, 285, 286, 325, 326, 632
SchI GAGTC 4 cut(s) 254, 263, 443, 456
ScrFI CCNGG 5 cut(s) 206, 274, 423, 502, 655
SduI GDGCHC 6 cut(s) 104, 113, 289, 329, 354, 852
SfaNI GCATC 1 cut(s) 858
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 4 cut(s) 19, 40, 127, 632
SmiMI CAYNNNNRTG 2 cut(s) 540, 1040
SmlI CTYRAG 4 cut(s) 215, 302, 308, 494
SmoI CTYRAG 4 cut(s) 215, 302, 308, 494
Sse9I AATT 5 cut(s) 7, 484, 548, 713, 878
SspMI CTAG 3 cut(s) 606, 710, 887
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 5 cut(s) 204, 272, 421, 500, 653
TaaI ACNGT 5 cut(s) 636, 771, 803, 908, 961
TaiI ACGT 2 cut(s) 84, 110
TaqI TCGA 6 cut(s) 11, 48, 267, 513, 638, 876
TaqII GACCGA 1 cut(s) 470
TasI AATT 5 cut(s) 7, 484, 548, 713, 878
TatI WGTACW 1 cut(s) 1026
TauI GCSGC 4 cut(s) 75, 343, 394, 925
TfiI GAWTC 5 cut(s) 45, 403, 626, 744, 896
Tru1I TTAA 3 cut(s) 59, 483, 843
Tru9I TTAA 3 cut(s) 59, 483, 843
TscAI CASTG 3 cut(s) 359, 913, 1066
TseI GCWGC 6 cut(s) 439, 490, 737, 833, 836, 925
TspDTI ATGAA 1 cut(s) 818
TspRI CASTG 3 cut(s) 359, 913, 1066
VneI GTGCAC 1 cut(s) 350
VpaK11BI GGWCC 4 cut(s) 19, 40, 127, 632
VspI ATTAAT 1 cut(s) 483
XapI RAATTY 2 cut(s) 7, 878
XmiI GTMKAC 1 cut(s) 638
XspI CTAG 3 cut(s) 606, 710, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.