Prupe.2G254900_v2.0.a1

MSP (Major sperm protein) domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0016395
Physical Location & Seq
Forward (+)
26769087 .. 26770777
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G254900.1

Sequence Viewer

Length: 1212 bp
ATGCCAATGTCCTTCTCCCCCTCCCACAAAACTCCTCCTCCTTCATTCAGACAATTCACAAATCTCCAGGCCTGTTATCCAATGGAGAAAAAACTAGTACAAGTTTCAGAGCAAGAGGTTCGCATAGATTTTGCACTCAACTGCAAATGCCGAGCTAACGTGCGCCTGACCTCCCTCTGCGCCACCGCCCCTGTAGCCTTCAAGGTCCAGACATCTTCGCCTCACAAGTTCCTCGTCAAACCACCCACCGGTTTAATCCCTCCGTTATGCCACGTTACCTTCCAGGTCATCCTCAAGCCTCAGGCCCAGCTTCCGCCCACCTTTCCTCGCTCGCCGTCCGACCGATTCCTCATCAGAACCTCCGAGTTCACCCGCGACTCCGAGTCCGAGTCAATCAACCAGTGGTTCTCCTCCTGTTCCCGCGGCTCCTCTCACGACCTCAAGCTCAAGGTTGCCTTCGTGGGCCCCTTTCTTCTGCGCCACGCTGTCACTTGCGGGGACTACAACGCCGCCCGGAATATAATCAAGCGGCAGAGAACGATTCTCACCGAGTTTCCACCCGGGGATGCTGAGTCGCTCCTTCAAGTTGCGACCGAGTTGGTTAACCCAGAGGACATGATTAACTTGCTGCTTGAAGCTGGATTGAGGATCGACGCACGTGTGAGGTCCGAGCAAGTAAATTACGAGGTGGATTCCAAATGGGCATCCAACGGACACGAGGAGCCACAAGTGGCAGCGGCCTGCGATCGATTGGATTTTGGTATGGGCTGCCGGAATGGAGTATGTGATAACCGCCGTTCGATCCAACATGCTGCTTGTGATAAGGATAAGCTAGAAATGGGAGAACTAGTATTGATGGCGGCAAGACGAGGAGATTTGAAGCATGTTGAATTGCTGTTGCAAAATGGCGCAGATATAAATTGCTGTGATCAATATGGCTTGACATCCCTGCACGCCTCAGCCATTAAAGGCCACAAGGATATTGCTCTGATGCTCATCGAATTTGGGTTGGAATTGGAATGCCGAGACAGCGAAGGCCATGCGCCCTTGCATTTGGCTGTTGTGGGCGGGAGCTTGGAGACCGTTGAGATGTTGGTTCAGAATGGGGCTAATGTCAATGCCAAGAGCAACAGCGGCGCCACCCCTCTGTACATGGCTACGGCAATGGGGTATGATGATATCACCGAGTTTCTTATTAGCAGAGGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

404

Amino Acids

44.44

Weight (kDa)

6.32

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016395)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24970 FvH4_7g24970
malus_domestica MD01G1157100.v1.1
prunus_persica Prupe.2G254900_v2.0.a1
pyrus_communis pycom01g17390
rosa_chinensis RchiOBHm_Chr1g0370871
rosa_laevigata RLG00000027003
rosa_multiflora Rmu_sc0009474.1_g000017
rosa_roxburghii Rroxscaffold_4G00286170
rosa_rugosa Rorug01G0360000
rosa_samantha Rh1AG369700 Rh1BG333300 Rh1CG346700 Rh1DG364500
rosa_wichuraiana Rw1G032550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1136
AccII CGCG 2 cut(s) 375, 423
AclWI GGATC 2 cut(s) 656, 796
AcsI RAATTY 1 cut(s) 1001
AcvI CACGTG 1 cut(s) 659
AcyI GRCGYC 1 cut(s) 1137
AfaI GTAC 2 cut(s) 99, 1151
AfiI CCNNNNNNNGG 1 cut(s) 248
AflIII ACRYGT 1 cut(s) 658
AgeI ACCGGT 1 cut(s) 248
AgsI TTSAA 5 cut(s) 202, 584, 635, 880, 890
AhdI GACNNNNNGTC 1 cut(s) 382
AhlI ACTAGT 2 cut(s) 94, 847
AjnI CCWGG 2 cut(s) 66, 282
AluBI AGCT 6 cut(s) 155, 310, 445, 638, 832, 1074
AluI AGCT 6 cut(s) 155, 310, 445, 638, 832, 1074
Alw26I GTCTC 2 cut(s) 1020, 1073
AlwI GGATC 2 cut(s) 656, 796
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 6 cut(s) 69, 303, 463, 738, 970, 1036
ApaI GGGCCC 1 cut(s) 467
ApeKI GCWGC 4 cut(s) 628, 734, 768, 812
ApoI RAATTY 1 cut(s) 1001
AsiGI ACCGGT 1 cut(s) 248
AspLEI GCGC 6 cut(s) 165, 182, 480, 911, 1045, 1139
AspS9I GGNCC 5 cut(s) 205, 304, 463, 464, 666
AsuC2I CCSGG 3 cut(s) 514, 561, 562
AsuHPI GGTGA 3 cut(s) 361, 538, 1174
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 2 cut(s) 205, 666
AxyI CCTNAGG 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 1136
BanII GRGCYC 1 cut(s) 467
BauI CACGAG 1 cut(s) 716
BbrPI CACGTG 1 cut(s) 659
BbvCI CCTCAGC 1 cut(s) 958
BbvI GCAGC 4 cut(s) 615, 746, 755, 799
BccI CCATC 1 cut(s) 850
BceAI ACGGC 3 cut(s) 319, 780, 1176
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 1022, 1056
BciT130I CCWGG 2 cut(s) 68, 284
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 3 cut(s) 514, 561, 562
BcoDI GTCTC 2 cut(s) 1020, 1073
BcuI ACTAGT 2 cut(s) 94, 847
BfaI CTAG 3 cut(s) 95, 833, 848
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 1140
Bme1390I CCNGG 5 cut(s) 68, 284, 514, 561, 562
Bme18I GGWCC 2 cut(s) 205, 666
BmeRI GACNNNNNGTC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 5 cut(s) 205, 304, 463, 464, 666
BmiI GGNNCC 5 cut(s) 427, 465, 466, 723, 1138
BmrFI CCNGG 5 cut(s) 68, 284, 514, 561, 562
BmsI GCATC 3 cut(s) 556, 713, 981
BpmI CTGGAG 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 958
BpuEI CTTGAG 3 cut(s) 278, 425, 431
BpuMI CCSGG 3 cut(s) 514, 561, 562
Bsa29I ATCGAT 1 cut(s) 748
BsaAI YACGTR 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 995
BsaHI GRCGYC 1 cut(s) 1137
BsaI GGTCTC 1 cut(s) 1073
BsaJI CCNNGG 3 cut(s) 421, 560, 561
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 834, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 400
Bse21I CCTNAGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 1170
Bse8I GATNNNNATC 1 cut(s) 995
BseBI CCWGG 2 cut(s) 68, 284
BseCI ATCGAT 1 cut(s) 748
BseDI CCNNGG 3 cut(s) 421, 560, 561
BseGI GGATG 4 cut(s) 288, 571, 704, 944
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 3 cut(s) 314, 561, 972
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 6 cut(s) 24, 27, 400, 418, 734, 885
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 4 cut(s) 615, 746, 755, 799
BseYI CCCAGC 1 cut(s) 306
BsgI GTGCAG 1 cut(s) 935
Bsh1236I CGCG 2 cut(s) 375, 423
Bsh1285I CGRYCG 3 cut(s) 343, 594, 748
BshFI GGCC 6 cut(s) 71, 305, 465, 740, 972, 1038
BshNI GGYRCC 1 cut(s) 1136
BshTI ACCGGT 1 cut(s) 248
BshVI ATCGAT 1 cut(s) 748
BsiEI CGRYCG 3 cut(s) 343, 594, 748
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 4 cut(s) 249, 514, 561, 772
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 2 cut(s) 1020, 1073
BsmFI GGGAC 1 cut(s) 512
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 6 cut(s) 71, 305, 465, 740, 972, 1038
Bso31I GGTCTC 1 cut(s) 1073
BsoBI CYCGRG 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 463
Bsp1286I GDGCHC 1 cut(s) 467
Bsp1407I TGTACA 1 cut(s) 1149
Bsp143I GATC 4 cut(s) 648, 745, 801, 928
BspANI GGCC 6 cut(s) 71, 305, 465, 740, 972, 1038
BspCNI CTCAG 3 cut(s) 313, 562, 971
BspDI ATCGAT 1 cut(s) 748
BspFNI CGCG 2 cut(s) 375, 423
BspLI GGNNCC 5 cut(s) 427, 465, 466, 723, 1138
BspPI GGATC 2 cut(s) 656, 796
BspT107I GGYRCC 1 cut(s) 1136
BspTNI GGTCTC 1 cut(s) 1073
BsrDI GCAATG 1 cut(s) 1170
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 1149
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 421, 560, 561
BssMI GATC 4 cut(s) 648, 745, 801, 928
BssNI GRCGYC 1 cut(s) 1137
BssSI CACGAG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 716
Bst2UI CCWGG 2 cut(s) 68, 284
Bst4CI ACNGT 1 cut(s) 1084
BstACI GRCGYC 1 cut(s) 1137
BstAUI TGTACA 1 cut(s) 1149
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 3 cut(s) 332, 742, 954
BstDEI CTNAG 3 cut(s) 300, 570, 958
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 4 cut(s) 288, 571, 704, 944
BstFNI CGCG 2 cut(s) 375, 423
BstH2I RGCGCY 1 cut(s) 1140
BstHHI GCGC 6 cut(s) 165, 182, 480, 911, 1045, 1139
BstKTI GATC 4 cut(s) 651, 748, 804, 931
BstMAI GTCTC 2 cut(s) 1020, 1073
BstMBI GATC 4 cut(s) 648, 745, 801, 928
BstMCI CGRYCG 3 cut(s) 343, 594, 748
BstMWI GCNNNNNNNGC 3 cut(s) 194, 1029, 1134
BstNI CCWGG 2 cut(s) 68, 284
BstNSI RCATGY 2 cut(s) 812, 887
BstSCI CCNGG 5 cut(s) 66, 282, 512, 559, 560
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 467
BstUI CGCG 2 cut(s) 375, 423
BstV1I GCAGC 4 cut(s) 615, 746, 755, 799
Bsu15I ATCGAT 1 cut(s) 748
Bsu36I CCTNAGG 1 cut(s) 300
BsuRI GGCC 6 cut(s) 71, 305, 465, 740, 972, 1038
BsuTUI ATCGAT 1 cut(s) 748
BtgI CCRYGG 1 cut(s) 421
BtsCI GGATG 4 cut(s) 288, 571, 704, 944
BtsIMutI CAGTG 1 cut(s) 407
Cac8I GCNNGC 3 cut(s) 332, 742, 954
CfoI GCGC 6 cut(s) 165, 182, 480, 911, 1045, 1139
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 5 cut(s) 205, 304, 463, 464, 666
Cfr42I CCGCGG 1 cut(s) 424
Cfr9I CCCGGG 1 cut(s) 560
ClaI ATCGAT 1 cut(s) 748
CseI GACGC 1 cut(s) 662
Csp6I GTAC 2 cut(s) 98, 1150
CspAI ACCGGT 1 cut(s) 248
CviAII CATG 5 cut(s) 616, 809, 884, 1040, 1153
CviQI GTAC 2 cut(s) 98, 1150
DdeI CTNAG 3 cut(s) 300, 570, 958
DinI GGCGCC 1 cut(s) 1138
DpnI GATC 4 cut(s) 650, 747, 803, 930
DpnII GATC 4 cut(s) 648, 745, 801, 928
DriI GACNNNNNGTC 1 cut(s) 382
Eam1105I GACNNNNNGTC 1 cut(s) 382
EciI GGCGGA 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 1073
Eco32I GATATC 1 cut(s) 1180
Eco47I GGWCC 2 cut(s) 205, 666
Eco72I CACGTG 1 cut(s) 659
Eco81I CCTNAGG 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 66, 282
EcoRV GATATC 1 cut(s) 1180
EcoT38I GRGCYC 1 cut(s) 467
EgeI GGCGCC 1 cut(s) 1138
EheI GGCGCC 1 cut(s) 1138
FaeI CATG 5 cut(s) 619, 812, 887, 1043, 1156
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 1 cut(s) 512
FatI CATG 5 cut(s) 615, 808, 883, 1039, 1152
FauI CCCGC 4 cut(s) 380, 428, 488, 1061
FbaI TGATCA 1 cut(s) 928
FokI GGATG 4 cut(s) 275, 578, 691, 931
FriOI GRGCYC 1 cut(s) 467
FspBI CTAG 3 cut(s) 95, 833, 848
GlaI GCGC 6 cut(s) 164, 181, 479, 910, 1044, 1138
GsaI CCCAGC 1 cut(s) 310
GsuI CTGGAG 1 cut(s) 50
HaeII RGCGCY 1 cut(s) 1140
HaeIII GGCC 6 cut(s) 71, 305, 465, 740, 972, 1038
HapII CCGG 4 cut(s) 249, 514, 561, 772
HgaI GACGC 1 cut(s) 662
HhaI GCGC 6 cut(s) 165, 182, 480, 911, 1045, 1139
Hin1I GRCGYC 1 cut(s) 1137
Hin1II CATG 5 cut(s) 619, 812, 887, 1043, 1156
Hin6I GCGC 6 cut(s) 163, 180, 478, 909, 1043, 1137
HinP1I GCGC 6 cut(s) 163, 180, 478, 909, 1043, 1137
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 7 cut(s) 345, 377, 383, 389, 541, 572, 692
HpaI GTTAAC 1 cut(s) 604
HpaII CCGG 4 cut(s) 249, 514, 561, 772
HphI GGTGA 3 cut(s) 361, 538, 1174
Hpy166II GTNNAC 2 cut(s) 369, 604
Hpy188III TCNNGA 2 cut(s) 208, 434
Hpy8I GTNNAC 2 cut(s) 369, 604
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 7 cut(s) 22, 51, 208, 289, 466, 590, 1028
HpyCH4III ACNGT 1 cut(s) 1084
HpyCH4IV ACGT 3 cut(s) 159, 273, 658
HpyCH4V TGCA 5 cut(s) 134, 144, 901, 952, 1051
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 1029, 1134
HpyF3I CTNAG 3 cut(s) 300, 570, 958
HpySE526I ACGT 3 cut(s) 159, 273, 658
Hsp92I GRCGYC 1 cut(s) 1137
Hsp92II CATG 5 cut(s) 619, 812, 887, 1043, 1156
HspAI GCGC 6 cut(s) 163, 180, 478, 909, 1043, 1137
KasI GGCGCC 1 cut(s) 1136
Ksp22I TGATCA 1 cut(s) 928
KspAI GTTAAC 1 cut(s) 604
KspI CCGCGG 1 cut(s) 424
Kzo9I GATC 4 cut(s) 648, 745, 801, 928
LmnI GCTCC 4 cut(s) 431, 582, 721, 1071
Lsp1109I GCAGC 4 cut(s) 615, 746, 755, 799
LweI GCATC 3 cut(s) 556, 713, 981
MaeI CTAG 3 cut(s) 95, 833, 848
MaeII ACGT 3 cut(s) 159, 273, 658
MaeIII GTNAC 2 cut(s) 274, 487
MalI GATC 4 cut(s) 650, 747, 803, 930
MboI GATC 4 cut(s) 648, 745, 801, 928
MboII GAAGA 2 cut(s) 207, 464
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 6 cut(s) 53, 679, 890, 919, 1001, 1013
Mly113I GGCGCC 1 cut(s) 1137
MlyI GAGTC 4 cut(s) 371, 392, 398, 581
MmeI TCCRAC 4 cut(s) 363, 732, 829, 990
MseI TTAA 5 cut(s) 254, 603, 621, 966, 1210
MspA1I CMGCKG 3 cut(s) 423, 737, 1134
MspI CCGG 4 cut(s) 249, 514, 561, 772
MspR9I CCNGG 5 cut(s) 68, 284, 514, 561, 562
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 2 cut(s) 68, 284
MvnI CGCG 2 cut(s) 375, 423
MwoI GCNNNNNNNGC 3 cut(s) 194, 1029, 1134
NarI GGCGCC 1 cut(s) 1137
NciI CCSGG 3 cut(s) 514, 561, 562
NdeII GATC 4 cut(s) 648, 745, 801, 928
NlaIII CATG 5 cut(s) 619, 812, 887, 1043, 1156
NlaIV GGNNCC 5 cut(s) 427, 465, 466, 723, 1138
NmeAIII GCCGAG 2 cut(s) 176, 1049
NmuCI GTSAC 1 cut(s) 487
NspI RCATGY 2 cut(s) 812, 887
PceI AGGCCT 1 cut(s) 71
PctI GAATGC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 345, 541, 692
PinAI ACCGGT 1 cut(s) 248
Ple19I CGATCG 1 cut(s) 748
PleI GAGTC 4 cut(s) 371, 391, 397, 580
PluTI GGCGCC 1 cut(s) 1140
PmaCI CACGTG 1 cut(s) 659
PmlI CACGTG 1 cut(s) 659
PpsI GAGTC 4 cut(s) 371, 391, 397, 580
Ppu21I YACGTR 1 cut(s) 659
Psp6I CCWGG 2 cut(s) 66, 282
PspCI CACGTG 1 cut(s) 659
PspFI CCCAGC 1 cut(s) 306
PspGI CCWGG 2 cut(s) 66, 282
PspN4I GGNNCC 5 cut(s) 427, 465, 466, 723, 1138
PspOMI GGGCCC 1 cut(s) 463
PspPI GGNCC 5 cut(s) 205, 304, 463, 464, 666
PvuI CGATCG 1 cut(s) 748
RsaI GTAC 2 cut(s) 99, 1151
RsaNI GTAC 2 cut(s) 98, 1150
SacII CCGCGG 1 cut(s) 424
SaqAI TTAA 5 cut(s) 254, 603, 621, 966, 1210
Sau3AI GATC 4 cut(s) 648, 745, 801, 928
Sau96I GGNCC 5 cut(s) 205, 304, 463, 464, 666
SchI GAGTC 4 cut(s) 371, 392, 398, 581
ScrFI CCNGG 5 cut(s) 68, 284, 514, 561, 562
SduI GDGCHC 1 cut(s) 467
SfaNI GCATC 3 cut(s) 556, 713, 981
SfcI CTRYAG 1 cut(s) 192
SfoI GGCGCC 1 cut(s) 1138
Sfr303I CCGCGG 1 cut(s) 424
SgrBI CCGCGG 1 cut(s) 424
SinI GGWCC 2 cut(s) 205, 666
SmaI CCCGGG 1 cut(s) 562
SmlI CTYRAG 3 cut(s) 293, 440, 446
SmoI CTYRAG 3 cut(s) 293, 440, 446
SpeI ACTAGT 2 cut(s) 94, 847
Sse9I AATT 6 cut(s) 53, 679, 890, 919, 1001, 1013
SseBI AGGCCT 1 cut(s) 71
SspDI GGCGCC 1 cut(s) 1136
SspMI CTAG 3 cut(s) 95, 833, 848
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 5 cut(s) 66, 282, 512, 559, 560
TaaI ACNGT 1 cut(s) 1084
TaiI ACGT 3 cut(s) 162, 276, 661
TaqI TCGA 4 cut(s) 651, 748, 800, 999
TaqII GACCGA 2 cut(s) 357, 608
TasI AATT 6 cut(s) 53, 679, 890, 919, 1001, 1013
TatI WGTACW 2 cut(s) 97, 1149
TauI GCSGC 6 cut(s) 426, 512, 532, 740, 863, 1137
TfiI GAWTC 3 cut(s) 345, 541, 692
Tru1I TTAA 5 cut(s) 254, 603, 621, 966, 1210
Tru9I TTAA 5 cut(s) 254, 603, 621, 966, 1210
TscAI CASTG 1 cut(s) 407
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 4 cut(s) 628, 734, 768, 812
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 1 cut(s) 33
TspGWI ACGGA 2 cut(s) 252, 726
TspMI CCCGGG 1 cut(s) 560
TspRI CASTG 1 cut(s) 407
VpaK11BI GGWCC 2 cut(s) 205, 666
XapI RAATTY 1 cut(s) 1001
XceI RCATGY 2 cut(s) 812, 887
XmaI CCCGGG 1 cut(s) 560
XspI CTAG 3 cut(s) 95, 833, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.