MD00G1006200.v1.1

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0020381
Physical Location & Seq
Forward (+)
824605 .. 825324
720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1006200.v1.1.491

Sequence Viewer

Length: 720 bp
ATGAAGTTTTTCGCCACCCCACCGGTTCATGGTTCCTTGGCTAATCAGATTGTGCACCCTGCAGATCTTTGCTTTAAATTGCCAAAAAACGTGAGTTTGGAGGAAGGGGCAATGTGTGAGCCCTTGAGTATTGGGGTTCACGCTTGTCGGCGAGCCAATGTTGGTCCCGAAACAACTGTTCTGATCACCGGAGCAGGGCCTATTGGTCTCGTTTCAGTTTTAGCCGCTCGTGCTTTCGGGGCACCAAGAATTGTGATTGTGGATATGGATGACAAGCGTCTAGCAATGGCAAAGTCTCTCGGTGCTGATGAAACCGTGAAAGTTTCGACAAAAATGGAGGACTTAGATGATGAAGTTGCTGAAATTAAAAAAGCCATGATCTCCGAAGTGGATGTGACCTTCGATTGTGTGGGTTTCAACAAAACCATGTCGACCGGCCTCAATACTACTCGTCCGGGCGGAAAAGTCTGCCTGGTAGGAATGGGACACGGTGTGATGACGGTGCCTCTGACTCCCGCTGCTGCTAGGGAGGTTGACGTTGTTGGAGTTTTCCGATACCAGAACACATGGCCGCTTTGCCTCGAGTTTTTGAGAAGTGGGAAGATCGATGTGAAGCCGCTTATCACGCACCGTTTTGGATTTACTGAGAATGAGGTGGAAGAAGCGTTTGCAACAAGTGCTCGTGGGGGTAACGCCATCAAAGTGATGTTCAAATTGTAA

Protein Analysis

240

Amino Acids

25.63

Weight (kDa)

6.52

Isoelectric Point (pI)

22.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_dehyd_C PF16912 36 - 165 3e-09 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 67 - 197 4.4e-29 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 100 - 230 1.1e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020381)

Species Orthologous Gene IDs
malus_domestica MD00G1006200.v1.1 MD01G1195100.v1.1
pyrus_communis pycom01g20520 pycom01g20570 pycom01g20590 pycom07g24120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 241, 502
AccBSI CCGCTC 1 cut(s) 227
AccI GTMKAC 1 cut(s) 431
AciI CCGC 5 cut(s) 225, 459, 516, 572, 617
AcoI YGGCCR 1 cut(s) 569
AdeI CACNNNGTG 1 cut(s) 493
AfiI CCNNNNNNNGG 2 cut(s) 29, 195
AgeI ACCGGT 1 cut(s) 22
AgsI TTSAA 2 cut(s) 418, 712
AjnI CCWGG 1 cut(s) 471
Alw21I GWGCWC 2 cut(s) 57, 682
Alw26I GTCTC 2 cut(s) 212, 300
Alw44I GTGCAC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 581
AoxI GGCC 3 cut(s) 197, 436, 569
ApaLI GTGCAC 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 518, 521
AsiGI ACCGGT 1 cut(s) 22
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 164, 197
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 581
AvaII GGWCC 1 cut(s) 164
BaeGI GKGCMC 2 cut(s) 57, 244
BanI GGYRCC 2 cut(s) 241, 502
BanII GRGCYC 1 cut(s) 123
BauI CACGAG 2 cut(s) 228, 681
Bbv12I GWGCWC 2 cut(s) 57, 682
BbvI GCAGC 2 cut(s) 505, 508
BccI CCATC 1 cut(s) 704
BciT130I CCWGG 1 cut(s) 473
BclI TGATCA 1 cut(s) 183
BcnI CCSGG 1 cut(s) 456
BcoDI GTCTC 2 cut(s) 212, 300
BfaI CTAG 2 cut(s) 281, 525
BfmI CTRYAG 1 cut(s) 60
BglII AGATCT 1 cut(s) 64
BisI GCNGC 5 cut(s) 225, 519, 522, 572, 617
BlsI GCNGC 5 cut(s) 226, 520, 523, 573, 618
Bme1390I CCNGG 2 cut(s) 456, 473
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 581
BmgT120I GGNCC 2 cut(s) 164, 197
BmiI GGNNCC 4 cut(s) 34, 166, 243, 504
BmrFI CCNGG 2 cut(s) 456, 473
BoxI GACNNNNGTC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 145
BpuMI CCSGG 1 cut(s) 456
Bsa29I ATCGAT 1 cut(s) 606
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 2 cut(s) 22, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 195
Bse118I RCCGGY 2 cut(s) 22, 434
Bse3DI GCAATG 2 cut(s) 117, 291
BseBI CCWGG 1 cut(s) 473
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 2 cut(s) 274, 397
BseLI CCNNNNNNNGG 2 cut(s) 29, 195
BseMI GCAATG 2 cut(s) 117, 291
BseMII CTCAG 1 cut(s) 636
BseSI GKGCMC 2 cut(s) 57, 244
BseXI GCAGC 2 cut(s) 505, 508
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 199, 438, 571
BshNI GGYRCC 2 cut(s) 241, 502
BshTI ACCGGT 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 435
BsiHKAI GWGCWC 2 cut(s) 57, 682
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 4 cut(s) 23, 189, 435, 455
BslFI GGGAC 2 cut(s) 150, 498
BslI CCNNNNNNNGG 2 cut(s) 29, 195
BsmAI GTCTC 2 cut(s) 212, 300
BsmFI GGGAC 2 cut(s) 150, 498
BsnI GGCC 3 cut(s) 199, 438, 571
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 581
Bsp1286I GDGCHC 4 cut(s) 57, 123, 244, 682
Bsp143I GATC 4 cut(s) 64, 183, 378, 603
BspACI CCGC 5 cut(s) 225, 459, 516, 572, 617
BspANI GGCC 3 cut(s) 199, 438, 571
BspCNI CTCAG 1 cut(s) 637
BspDI ATCGAT 1 cut(s) 606
BspLI GGNNCC 4 cut(s) 34, 166, 243, 504
BspMAI CTGCAG 1 cut(s) 64
BspT107I GGYRCC 2 cut(s) 241, 502
BspTNI GGTCTC 1 cut(s) 212
BsrBI CCGCTC 1 cut(s) 227
BsrDI GCAATG 2 cut(s) 117, 291
BsrFI RCCGGY 2 cut(s) 22, 434
BssAI RCCGGY 2 cut(s) 22, 434
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 4 cut(s) 64, 183, 378, 603
BssSI CACGAG 2 cut(s) 228, 681
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 2 cut(s) 228, 681
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 5 cut(s) 178, 316, 491, 502, 632
BstAPI GCANNNNNTGC 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 2 cut(s) 343, 645
BstF5I GGATG 2 cut(s) 274, 397
BstKTI GATC 4 cut(s) 67, 186, 381, 606
BstMAI GTCTC 2 cut(s) 212, 300
BstMBI GATC 4 cut(s) 64, 183, 378, 603
BstMCI CGRYCG 1 cut(s) 435
BstMWI GCNNNNNNNGC 4 cut(s) 230, 239, 625, 677
BstNI CCWGG 1 cut(s) 473
BstPAI GACNNNNGTC 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 454, 471
BstSFI CTRYAG 1 cut(s) 60
BstSLI GKGCMC 2 cut(s) 57, 244
BstV1I GCAGC 2 cut(s) 505, 508
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 3 cut(s) 199, 438, 571
BsuTUI ATCGAT 1 cut(s) 606
BtsCI GGATG 2 cut(s) 274, 397
Cac8I GCNNGC 1 cut(s) 153
Cfr10I RCCGGY 2 cut(s) 22, 434
Cfr13I GGNCC 2 cut(s) 164, 197
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 1 cut(s) 266
CspAI ACCGGT 1 cut(s) 22
CviAII CATG 4 cut(s) 29, 376, 427, 567
CviJI RGCY 9 cut(s) 41, 121, 155, 199, 224, 374, 438, 571, 616
CviKI_1 RGCY 9 cut(s) 41, 121, 155, 199, 224, 374, 438, 571, 616
DdeI CTNAG 2 cut(s) 343, 645
DpnI GATC 4 cut(s) 66, 185, 380, 605
DpnII GATC 4 cut(s) 64, 183, 378, 603
DraI TTTAAA 1 cut(s) 76
DraIII CACNNNGTG 1 cut(s) 493
EaeI YGGCCR 1 cut(s) 569
EciI GGCGGA 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 4 cut(s) 32, 379, 430, 570
FaiI YATR 5 cut(s) 30, 266, 377, 428, 568
FaqI GGGAC 2 cut(s) 150, 498
FatI CATG 4 cut(s) 28, 375, 426, 566
FauI CCCGC 1 cut(s) 523
FbaI TGATCA 1 cut(s) 183
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 5 cut(s) 225, 519, 522, 572, 617
FokI GGATG 2 cut(s) 281, 404
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 5 cut(s) 225, 519, 522, 572, 617
FspBI CTAG 2 cut(s) 281, 525
GluI GCNGC 5 cut(s) 225, 519, 522, 572, 617
HaeIII GGCC 3 cut(s) 199, 438, 571
HapII CCGG 4 cut(s) 23, 189, 435, 455
HgaI GACGC 1 cut(s) 266
Hin1II CATG 4 cut(s) 32, 379, 430, 570
HincII GTYRAC 2 cut(s) 432, 535
HindII GTYRAC 2 cut(s) 432, 535
HinfI GANTC 1 cut(s) 511
HpaII CCGG 4 cut(s) 23, 189, 435, 455
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 4 cut(s) 55, 139, 432, 535
Hpy188I TCNGA 5 cut(s) 48, 183, 385, 510, 554
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 4 cut(s) 55, 139, 432, 535
HpyAV CCTTC 2 cut(s) 98, 409
HpyCH4III ACNGT 5 cut(s) 178, 316, 491, 502, 632
HpyCH4IV ACGT 2 cut(s) 90, 537
HpyCH4V TGCA 3 cut(s) 55, 62, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 239, 625, 677
HpyF3I CTNAG 2 cut(s) 343, 645
HpySE526I ACGT 2 cut(s) 90, 537
Hsp92II CATG 4 cut(s) 32, 379, 430, 570
Ksp22I TGATCA 1 cut(s) 183
Kzo9I GATC 4 cut(s) 64, 183, 378, 603
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 9 cut(s) 36, 72, 180, 202, 448, 458, 468, 485, 572
Lsp1109I GCAGC 2 cut(s) 505, 508
MaeI CTAG 2 cut(s) 281, 525
MaeII ACGT 2 cut(s) 90, 537
MaeIII GTNAC 2 cut(s) 394, 689
MalI GATC 4 cut(s) 66, 185, 380, 605
MbiI CCGCTC 1 cut(s) 227
MboI GATC 4 cut(s) 64, 183, 378, 603
MboII GAAGA 2 cut(s) 613, 671
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 4 cut(s) 57, 123, 244, 682
MluCI AATT 4 cut(s) 77, 249, 363, 713
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 7 cut(s) 94, 331, 449, 516, 523, 590, 646
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 75, 366
MslI CAYNNNNRTG 1 cut(s) 701
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 4 cut(s) 23, 189, 435, 455
MspR9I CCNGG 2 cut(s) 456, 473
MvaI CCWGG 1 cut(s) 473
MwoI GCNNNNNNNGC 4 cut(s) 230, 239, 625, 677
NciI CCSGG 1 cut(s) 456
NdeII GATC 4 cut(s) 64, 183, 378, 603
NlaIII CATG 4 cut(s) 32, 379, 430, 570
NlaIV GGNNCC 4 cut(s) 34, 166, 243, 504
NmuCI GTSAC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 581
PdmI GAANNNNTTC 1 cut(s) 8
PinAI ACCGGT 1 cut(s) 22
PkrI GCNGC 5 cut(s) 226, 520, 523, 573, 618
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 4 cut(s) 34, 166, 243, 504
PspPI GGNCC 2 cut(s) 164, 197
PspXI VCTCGAGB 1 cut(s) 581
PstI CTGCAG 1 cut(s) 64
PsuI RGATCY 1 cut(s) 64
RseI CAYNNNNRTG 1 cut(s) 701
SalI GTCGAC 1 cut(s) 430
SaqAI TTAA 2 cut(s) 75, 366
SatI GCNGC 5 cut(s) 225, 519, 522, 572, 617
Sau3AI GATC 4 cut(s) 64, 183, 378, 603
Sau96I GGNCC 2 cut(s) 164, 197
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 2 cut(s) 456, 473
SduI GDGCHC 4 cut(s) 57, 123, 244, 682
SetI ASST 5 cut(s) 93, 401, 534, 540, 657
SfcI CTRYAG 1 cut(s) 60
Sfr274I CTCGAG 1 cut(s) 581
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 581
SmiMI CAYNNNNRTG 1 cut(s) 701
SmlI CTYRAG 2 cut(s) 124, 581
SmoI CTYRAG 2 cut(s) 124, 581
Sse9I AATT 4 cut(s) 77, 249, 363, 713
SsiI CCGC 5 cut(s) 225, 459, 516, 572, 617
SspMI CTAG 2 cut(s) 281, 525
StyD4I CCNGG 2 cut(s) 454, 471
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 5 cut(s) 178, 316, 491, 502, 632
TaiI ACGT 2 cut(s) 93, 540
TaqI TCGA 5 cut(s) 326, 402, 431, 582, 606
TasI AATT 4 cut(s) 77, 249, 363, 713
TauI GCSGC 3 cut(s) 227, 574, 619
Tru1I TTAA 2 cut(s) 75, 366
Tru9I TTAA 2 cut(s) 75, 366
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 2 cut(s) 518, 521
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 4 cut(s) 17, 17, 324, 366
VneI GTGCAC 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 281, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.