pycom01g20520

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020381
Physical Location & Seq
Forward (+)
18909125 .. 18910145
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20520.1

Sequence Viewer

Length: 720 bp
ATGAAGTTTTTCGCCACCCCTCCGGTTCATGGTTCCTTGGCAAATCAGATTGTGCATCCTGCGGATCTGTGCTTTAAGCTCCCGGAGAATGTGAGTTTGGAGGAAGGGGCAATGTGTGAACCCTTGAGTGTTGGGGTTCATGCTTGCCGGAGAGCCAATGTGGGACCGGAATCAACTGTCCTGATCATCGGAGCCGGCCCCATCGGTCTGGTTTCGGTGCTGGCTGCCCGTGCATTTGGGTCACCAAGAATTGTCATTGTGGATATGGATGACCAGCGTCTAGCCATGGCGAAGTCTCTCGGTGCTAATGACACTGTCAAAGTTTCGACAAAAATGGAGGATTTGGATGATGAAATTGCCCAGATTAAGACAGCAATGAAATCCAACGTGGATGTGAGCTTCGATTGTGTCGGTTTTAACAAAACCATGTCAACAGCTCTTGGTGCCACCCGTCCCGGCGGCAAAGTTTGCCTTGTCGGAATGGGACACGGCACGATGACAGTCCCACTTACTCCAGCTGCTGCCAGGGAGGTTGATGTTGTTGGAATTTTCCGGTACAAGAACACATGGCCGCTTTGCCTCGAGTTTTTGAGAAGTGGGAAGATCGACCTGAAGCCGCTCATTACGCACCGGTTCGGGTTTAACCAAGAGGAGGTGGAAGAAGCATTTGAAACCAGTGCCCGTGGGGGAAATGCCATTAAAGTCATGTTTATTTTGTGA

Protein Analysis

240

Amino Acids

25.62

Weight (kDa)

6.22

Isoelectric Point (pI)

25.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_dehyd_C PF16912 36 - 165 3.4e-09 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 67 - 197 7.5e-29 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 113 - 230 2.7e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020381)

Species Orthologous Gene IDs
malus_domestica MD00G1006200.v1.1 MD01G1195100.v1.1
pyrus_communis pycom01g20520 pycom01g20570 pycom01g20590 pycom07g24120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccBSI CCGCTC 1 cut(s) 619
AciI CCGC 4 cut(s) 62, 459, 572, 617
AclWI GGATC 1 cut(s) 72
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 1 cut(s) 546
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 2 cut(s) 29, 652
AgeI ACCGGT 1 cut(s) 630
AgsI TTSAA 1 cut(s) 671
AjnI CCWGG 1 cut(s) 524
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 4 cut(s) 79, 399, 437, 518
AluI AGCT 4 cut(s) 79, 399, 437, 518
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 1 cut(s) 72
AlwNI CAGNNNCTG 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 581
AoxI GGCC 2 cut(s) 196, 569
ApeKI GCWGC 3 cut(s) 224, 518, 521
ApoI RAATTY 1 cut(s) 546
AsiGI ACCGGT 1 cut(s) 630
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 164, 197
AsuC2I CCSGG 2 cut(s) 83, 456
AsuHPI GGTGA 1 cut(s) 234
AvaI CYCGRG 1 cut(s) 581
AvaII GGWCC 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 682
BanI GGYRCC 1 cut(s) 443
BbvI GCAGC 3 cut(s) 211, 505, 508
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 505
BciT130I CCWGG 1 cut(s) 526
BclI TGATCA 1 cut(s) 183
BcnI CCSGG 2 cut(s) 83, 456
BcoDI GTCTC 1 cut(s) 300
BfaI CTAG 1 cut(s) 281
BisI GCNGC 6 cut(s) 225, 460, 519, 522, 572, 617
BlsI GCNGC 6 cut(s) 226, 461, 520, 523, 573, 618
Bme1390I CCNGG 3 cut(s) 83, 456, 526
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 581
BmgT120I GGNCC 2 cut(s) 164, 197
BmiI GGNNCC 5 cut(s) 34, 165, 193, 199, 445
BmrFI CCNGG 3 cut(s) 83, 456, 526
BmsI GCATC 1 cut(s) 64
BoxI GACNNNNGTC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 145
BpuMI CCSGG 2 cut(s) 83, 456
BsaJI CCNNGG 4 cut(s) 36, 285, 525, 682
BsaWI WCCGGW 4 cut(s) 22, 166, 552, 630
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 652
Bse118I RCCGGY 2 cut(s) 194, 630
Bse1I ACTGG 1 cut(s) 675
Bse3DI GCAATG 2 cut(s) 117, 381
BseBI CCWGG 1 cut(s) 526
BseDI CCNNGG 4 cut(s) 36, 285, 525, 682
BseGI GGATG 4 cut(s) 55, 274, 352, 397
BseLI CCNNNNNNNGG 2 cut(s) 29, 652
BseMI GCAATG 2 cut(s) 117, 381
BseNI ACTGG 1 cut(s) 675
BseRI GAGGAG 1 cut(s) 665
BseSI GKGCMC 1 cut(s) 682
BseXI GCAGC 3 cut(s) 211, 505, 508
BshFI GGCC 2 cut(s) 198, 571
BshNI GGYRCC 1 cut(s) 443
BshTI ACCGGT 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 8 cut(s) 23, 83, 148, 167, 195, 456, 553, 631
BslFI GGGAC 4 cut(s) 177, 438, 488, 498
BslI CCNNNNNNNGG 2 cut(s) 29, 652
BsmAI GTCTC 1 cut(s) 300
BsmFI GGGAC 4 cut(s) 177, 438, 488, 498
BsnI GGCC 2 cut(s) 198, 571
BsoBI CYCGRG 1 cut(s) 581
Bsp1286I GDGCHC 1 cut(s) 682
Bsp143I GATC 3 cut(s) 64, 183, 603
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 4 cut(s) 62, 459, 572, 617
BspANI GGCC 2 cut(s) 198, 571
BspLI GGNNCC 5 cut(s) 34, 165, 193, 199, 445
BspPI GGATC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 619
BsrDI GCAATG 2 cut(s) 117, 381
BsrFI RCCGGY 2 cut(s) 194, 630
BsrI ACTGG 1 cut(s) 675
BssAI RCCGGY 2 cut(s) 194, 630
BssECI CCNNGG 4 cut(s) 36, 285, 525, 682
BssMI GATC 3 cut(s) 64, 183, 603
BssT1I CCWWGG 2 cut(s) 36, 285
Bst2UI CCWGG 1 cut(s) 526
Bst4CI ACNGT 3 cut(s) 178, 316, 502
BstAPI GCANNNNNTGC 1 cut(s) 468
BstC8I GCNNGC 3 cut(s) 145, 196, 222
BstDSI CCRYGG 2 cut(s) 285, 682
BstEII GGTNACC 1 cut(s) 240
BstF5I GGATG 4 cut(s) 55, 274, 352, 397
BstKTI GATC 3 cut(s) 67, 186, 606
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 3 cut(s) 64, 183, 603
BstMWI GCNNNNNNNGC 4 cut(s) 230, 443, 468, 625
BstNI CCWGG 1 cut(s) 526
BstPAI GACNNNNGTC 1 cut(s) 276
BstPI GGTNACC 1 cut(s) 240
BstSCI CCNGG 3 cut(s) 81, 454, 524
BstSLI GKGCMC 1 cut(s) 682
BstV1I GCAGC 3 cut(s) 211, 505, 508
BstX2I RGATCY 1 cut(s) 64
BstXI CCANNNNNNTGG 1 cut(s) 208
BstYI RGATCY 1 cut(s) 64
BsuRI GGCC 2 cut(s) 198, 571
BtgI CCRYGG 2 cut(s) 285, 682
BtsCI GGATG 4 cut(s) 55, 274, 352, 397
BtsIMutI CAGTG 2 cut(s) 312, 682
Cac8I GCNNGC 3 cut(s) 145, 196, 222
CaiI CAGNNNCTG 1 cut(s) 521
Cfr10I RCCGGY 2 cut(s) 194, 630
Cfr13I GGNCC 2 cut(s) 164, 197
CseI GACGC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 556
CspAI ACCGGT 1 cut(s) 630
CviAII CATG 6 cut(s) 29, 140, 286, 427, 567, 706
CviQI GTAC 1 cut(s) 556
DpnI GATC 3 cut(s) 66, 185, 605
DpnII GATC 3 cut(s) 64, 183, 603
EaeI YGGCCR 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 36, 285
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 581
Eco91I GGTNACC 1 cut(s) 240
EcoO65I GGTNACC 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 524
EcoT14I CCWWGG 2 cut(s) 36, 285
ErhI CCWWGG 2 cut(s) 36, 285
FaeI CATG 6 cut(s) 32, 143, 289, 430, 570, 709
FaiI YATR 7 cut(s) 30, 141, 266, 287, 428, 568, 707
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 4 cut(s) 177, 438, 488, 498
FatI CATG 6 cut(s) 28, 139, 285, 426, 566, 705
FbaI TGATCA 1 cut(s) 183
Fnu4HI GCNGC 6 cut(s) 225, 460, 519, 522, 572, 617
FokI GGATG 4 cut(s) 42, 281, 359, 404
Fsp4HI GCNGC 6 cut(s) 225, 460, 519, 522, 572, 617
FspBI CTAG 1 cut(s) 281
GluI GCNGC 6 cut(s) 225, 460, 519, 522, 572, 617
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 2 cut(s) 198, 571
HapII CCGG 8 cut(s) 23, 83, 148, 167, 195, 456, 553, 631
HgaI GACGC 1 cut(s) 266
Hin1II CATG 6 cut(s) 32, 143, 289, 430, 570, 709
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HinfI GANTC 1 cut(s) 170
HpaII CCGG 8 cut(s) 23, 83, 148, 167, 195, 456, 553, 631
HphI GGTGA 1 cut(s) 234
Hpy166II GTNNAC 2 cut(s) 119, 432
Hpy188I TCNGA 3 cut(s) 48, 191, 479
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 2 cut(s) 119, 432
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 3 cut(s) 178, 316, 502
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 2 cut(s) 55, 233
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 443, 468, 625
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 6 cut(s) 32, 143, 289, 430, 570, 709
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 183
Kzo9I GATC 3 cut(s) 64, 183, 603
LmnI GCTCC 2 cut(s) 84, 191
Lsp1109I GCAGC 3 cut(s) 211, 505, 508
LweI GCATC 1 cut(s) 64
MaeI CTAG 1 cut(s) 281
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 240
MalI GATC 3 cut(s) 66, 185, 605
MbiI CCGCTC 1 cut(s) 619
MboI GATC 3 cut(s) 64, 183, 603
MboII GAAGA 2 cut(s) 613, 671
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 682
MluCI AATT 3 cut(s) 249, 354, 546
MmeI TCCRAC 3 cut(s) 408, 457, 523
MnlI CCTC 7 cut(s) 30, 94, 331, 523, 590, 643, 646
MroNI GCCGGC 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 5 cut(s) 75, 366, 417, 642, 699
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 8 cut(s) 23, 83, 148, 167, 195, 456, 553, 631
MspR9I CCNGG 3 cut(s) 83, 456, 526
MvaI CCWGG 1 cut(s) 526
MwoI GCNNNNNNNGC 4 cut(s) 230, 443, 468, 625
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 2 cut(s) 83, 456
NcoI CCATGG 1 cut(s) 285
NdeII GATC 3 cut(s) 64, 183, 603
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 6 cut(s) 32, 143, 289, 430, 570, 709
NlaIV GGNNCC 5 cut(s) 34, 165, 193, 199, 445
NmuCI GTSAC 1 cut(s) 240
PaeR7I CTCGAG 1 cut(s) 581
PdiI GCCGGC 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 170
PflFI GACNNNGTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 81
PinAI ACCGGT 1 cut(s) 630
PkrI GCNGC 6 cut(s) 226, 461, 520, 523, 573, 618
PshAI GACNNNNGTC 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 524
PspEI GGTNACC 1 cut(s) 240
PspGI CCWGG 1 cut(s) 524
PspN4I GGNNCC 5 cut(s) 34, 165, 193, 199, 445
PspPI GGNCC 2 cut(s) 164, 197
PspXI VCTCGAGB 1 cut(s) 581
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 64
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
SaqAI TTAA 5 cut(s) 75, 366, 417, 642, 699
SatI GCNGC 6 cut(s) 225, 460, 519, 522, 572, 617
Sau3AI GATC 3 cut(s) 64, 183, 603
Sau96I GGNCC 2 cut(s) 164, 197
ScrFI CCNGG 3 cut(s) 83, 456, 526
SduI GDGCHC 1 cut(s) 682
SetI ASST 8 cut(s) 81, 390, 401, 439, 520, 534, 612, 657
SfaNI GCATC 1 cut(s) 64
Sfr274I CTCGAG 1 cut(s) 581
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 124, 581
SmoI CTYRAG 2 cut(s) 124, 581
Sse9I AATT 3 cut(s) 249, 354, 546
SsiI CCGC 4 cut(s) 62, 459, 572, 617
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 3 cut(s) 81, 454, 524
StyI CCWWGG 2 cut(s) 36, 285
TaaI ACNGT 3 cut(s) 178, 316, 502
TaiI ACGT 1 cut(s) 390
TaqI TCGA 4 cut(s) 326, 402, 582, 606
TaqII GACCGA 1 cut(s) 194
TasI AATT 3 cut(s) 249, 354, 546
TauI GCSGC 3 cut(s) 462, 574, 619
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 5 cut(s) 75, 366, 417, 642, 699
Tru9I TTAA 5 cut(s) 75, 366, 417, 642, 699
TscAI CASTG 2 cut(s) 319, 682
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 3 cut(s) 224, 518, 521
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 5 cut(s) 17, 17, 128, 366, 392
TspRI CASTG 2 cut(s) 319, 682
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 546
XhoI CTCGAG 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.