pycom07g24120

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0020381
Physical Location & Seq
Forward (+)
24900908 .. 24901846
939 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24120.2

Sequence Viewer

Length: 720 bp
ATGAAGTTTTTCGCCACCCCACCGGTTCATGGTTCCTTGGCTAATCAGATTGTGCATCCTGCGGATCTATGTTTTAAGCTGCCGGAGAACGTGAGCTTGGAGGAAGGGGCAATGTGCGAGCCCTTAAGTGTTGGGGTTCACGCTTGTCGTCGAGCCAATGTTGGTCCCGAAACAACTGTTCTGGTCATCGGAGCAGGGCCCATTGGTCTGGTTTCTGTTTTGGCCGCTCGTGCTTTTGGGGCACCAAGAATTGTCATTGTGGATATGGATGACCAGCGTCTAGCTATGGCAAAGTCTCTCGGCGCTGATGAAACCGGGAAAGTTTCGACAAAAATGGAGGACTTAGATGATGAAGTTGCCAAGATTAAAAAGGCCATGAATTCCGAAGTGGATGTGACATTCGATTGTGTTGGTTTCAATAAAACCATGTCGACTGGTCTCAACGCCACTCGCCCCGGCGGTAAAGTCTGCCTTGTCGGAATGGGACATGGCATGATGACTGTCCCTCTAACTCCAGCTGCTGCTAGGGAAGTTGATGTGGTTGGAGTTTTCCGGTACAAGAACACATGGCCACTTTGCCTCGATTTCTTGAGAACTGGGAAGATCGACGTGAAGCCGCTTATTACTCACCGGTTTGGATTCACCGAAAAGGAGGTGGAAGAAGCCTTTGCGACCAGTGCTCGTGGGGGTAACGCCATCAAAGTCATGTTTAATCTATAG

Protein Analysis

240

Amino Acids

25.58

Weight (kDa)

6.52

Isoelectric Point (pI)

19.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020381)

Species Orthologous Gene IDs
malus_domestica MD00G1006200.v1.1 MD01G1195100.v1.1
pyrus_communis pycom01g20520 pycom01g20570 pycom01g20590 pycom07g24120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 241
AccBSI CCGCTC 1 cut(s) 227
AccI GTMKAC 1 cut(s) 431
AciI CCGC 4 cut(s) 62, 225, 459, 617
AclWI GGATC 1 cut(s) 72
AcoI YGGCCR 2 cut(s) 222, 569
AcsI RAATTY 1 cut(s) 379
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 1 cut(s) 29
AflII CTTAAG 1 cut(s) 124
AgeI ACCGGT 2 cut(s) 22, 630
AgsI TTSAA 1 cut(s) 418
AjiI CACGTC 1 cut(s) 610
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 4 cut(s) 79, 96, 284, 518
AluI AGCT 4 cut(s) 79, 96, 284, 518
Alw21I GWGCWC 1 cut(s) 682
Alw26I GTCTC 2 cut(s) 300, 443
AlwI GGATC 1 cut(s) 72
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 4 cut(s) 197, 222, 372, 569
ApaI GGGCCC 1 cut(s) 201
ApeKI GCWGC 3 cut(s) 79, 518, 521
ApoI RAATTY 1 cut(s) 379
AsiGI ACCGGT 2 cut(s) 22, 630
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 3 cut(s) 164, 197, 198
AsuC2I CCSGG 2 cut(s) 316, 456
AsuHPI GGTGA 2 cut(s) 620, 634
AvaII GGWCC 1 cut(s) 164
BaeGI GKGCMC 2 cut(s) 201, 244
BalI TGGCCA 1 cut(s) 571
BanI GGYRCC 1 cut(s) 241
BanII GRGCYC 2 cut(s) 123, 201
BauI CACGAG 2 cut(s) 228, 681
Bbv12I GWGCWC 1 cut(s) 682
BbvI GCAGC 3 cut(s) 66, 505, 508
BccI CCATC 1 cut(s) 704
BcnI CCSGG 2 cut(s) 316, 456
BcoDI GTCTC 2 cut(s) 300, 443
BfaI CTAG 2 cut(s) 281, 525
BfmI CTRYAG 1 cut(s) 716
BfoI RGCGCY 1 cut(s) 306
BfrI CTTAAG 1 cut(s) 124
BisI GCNGC 5 cut(s) 80, 225, 519, 522, 617
BlsI GCNGC 5 cut(s) 81, 226, 520, 523, 618
Bme1390I CCNGG 2 cut(s) 316, 456
Bme18I GGWCC 1 cut(s) 164
BmgBI CACGTC 1 cut(s) 610
BmgT120I GGNCC 3 cut(s) 164, 197, 198
BmiI GGNNCC 4 cut(s) 34, 166, 199, 243
BmrFI CCNGG 2 cut(s) 316, 456
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 64
BmuI ACTGGG 1 cut(s) 606
BoxI GACNNNNGTC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 610
BpuMI CCSGG 2 cut(s) 316, 456
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 36, 454
BsaWI WCCGGW 3 cut(s) 22, 552, 630
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 2 cut(s) 22, 630
Bse1I ACTGG 3 cut(s) 439, 601, 675
Bse3DI GCAATG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 36, 454
BseGI GGATG 3 cut(s) 55, 274, 397
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 117
BseNI ACTGG 3 cut(s) 439, 601, 675
BseSI GKGCMC 2 cut(s) 201, 244
BseXI GCAGC 3 cut(s) 66, 505, 508
BshFI GGCC 4 cut(s) 199, 224, 374, 571
BshNI GGYRCC 1 cut(s) 241
BshTI ACCGGT 2 cut(s) 22, 630
BsiHKAI GWGCWC 1 cut(s) 682
BsiSI CCGG 6 cut(s) 23, 83, 315, 456, 553, 631
BslFI GGGAC 3 cut(s) 150, 488, 498
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 2 cut(s) 300, 443
BsmFI GGGAC 3 cut(s) 150, 488, 498
BsnI GGCC 4 cut(s) 199, 224, 374, 571
Bso31I GGTCTC 1 cut(s) 443
Bsp120I GGGCCC 1 cut(s) 197
Bsp1286I GDGCHC 4 cut(s) 123, 201, 244, 682
Bsp143I GATC 2 cut(s) 64, 603
BspACI CCGC 4 cut(s) 62, 225, 459, 617
BspANI GGCC 4 cut(s) 199, 224, 374, 571
BspLI GGNNCC 4 cut(s) 34, 166, 199, 243
BspPI GGATC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 241
BspTI CTTAAG 1 cut(s) 124
BspTNI GGTCTC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 117
BsrFI RCCGGY 2 cut(s) 22, 630
BsrI ACTGG 3 cut(s) 439, 601, 675
BssAI RCCGGY 2 cut(s) 22, 630
BssECI CCNNGG 2 cut(s) 36, 454
BssMI GATC 2 cut(s) 64, 603
BssSI CACGAG 2 cut(s) 228, 681
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 2 cut(s) 228, 681
Bst4CI ACNGT 2 cut(s) 178, 502
BstAFI CTTAAG 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 343
BstF5I GGATG 3 cut(s) 55, 274, 397
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 2 cut(s) 67, 606
BstMAI GTCTC 2 cut(s) 300, 443
BstMBI GATC 2 cut(s) 64, 603
BstMWI GCNNNNNNNGC 3 cut(s) 230, 239, 677
BstPAI GACNNNNGTC 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 314, 454
BstSFI CTRYAG 1 cut(s) 716
BstSLI GKGCMC 2 cut(s) 201, 244
BstV1I GCAGC 3 cut(s) 66, 505, 508
BstX2I RGATCY 1 cut(s) 64
BstXI CCANNNNNNTGG 1 cut(s) 208
BstYI RGATCY 1 cut(s) 64
BsuRI GGCC 4 cut(s) 199, 224, 374, 571
BtrI CACGTC 1 cut(s) 610
BtsCI GGATG 3 cut(s) 55, 274, 397
BtsIMutI CAGTG 1 cut(s) 682
Cac8I GCNNGC 1 cut(s) 119
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 2 cut(s) 22, 630
Cfr13I GGNCC 3 cut(s) 164, 197, 198
CseI GACGC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 556
CspAI ACCGGT 2 cut(s) 22, 630
CviAII CATG 7 cut(s) 29, 376, 427, 488, 493, 567, 706
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 1 cut(s) 343
DpnI GATC 2 cut(s) 66, 605
DpnII GATC 2 cut(s) 64, 603
EaeI YGGCCR 2 cut(s) 222, 569
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 123, 201
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 123, 201
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 7 cut(s) 32, 379, 430, 491, 496, 570, 709
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 3 cut(s) 150, 488, 498
FatI CATG 7 cut(s) 28, 375, 426, 487, 492, 566, 705
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 5 cut(s) 80, 225, 519, 522, 617
FokI GGATG 3 cut(s) 42, 281, 404
FriOI GRGCYC 2 cut(s) 123, 201
Fsp4HI GCNGC 5 cut(s) 80, 225, 519, 522, 617
FspBI CTAG 2 cut(s) 281, 525
GlaI GCGC 1 cut(s) 304
GluI GCNGC 5 cut(s) 80, 225, 519, 522, 617
GsuI CTGGAG 1 cut(s) 498
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 4 cut(s) 199, 224, 374, 571
HapII CCGG 6 cut(s) 23, 83, 315, 456, 553, 631
HgaI GACGC 1 cut(s) 266
HhaI GCGC 1 cut(s) 305
Hin1II CATG 7 cut(s) 32, 379, 430, 491, 496, 570, 709
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HinfI GANTC 1 cut(s) 639
HpaII CCGG 6 cut(s) 23, 83, 315, 456, 553, 631
HphI GGTGA 2 cut(s) 620, 634
Hpy166II GTNNAC 2 cut(s) 139, 432
Hpy188I TCNGA 4 cut(s) 48, 191, 385, 479
Hpy188III TCNNGA 2 cut(s) 167, 589
Hpy8I GTNNAC 2 cut(s) 139, 432
Hpy99I CGWCG 2 cut(s) 153, 611
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 2 cut(s) 178, 502
HpyCH4IV ACGT 2 cut(s) 90, 609
HpyCH4V TGCA 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 239, 677
HpyF3I CTNAG 1 cut(s) 343
HpySE526I ACGT 2 cut(s) 90, 609
Hsp92II CATG 7 cut(s) 32, 379, 430, 491, 496, 570, 709
HspAI GCGC 1 cut(s) 303
Kzo9I GATC 2 cut(s) 64, 603
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 3 cut(s) 66, 505, 508
LweI GCATC 1 cut(s) 64
MaeI CTAG 2 cut(s) 281, 525
MaeII ACGT 2 cut(s) 90, 609
MaeIII GTNAC 2 cut(s) 394, 689
MalI GATC 2 cut(s) 66, 605
MbiI CCGCTC 1 cut(s) 227
MboI GATC 2 cut(s) 64, 603
MboII GAAGA 2 cut(s) 613, 671
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 4 cut(s) 123, 201, 244, 682
MlsI TGGCCA 1 cut(s) 571
MluCI AATT 2 cut(s) 249, 379
MluNI TGGCCA 1 cut(s) 571
MmeI TCCRAC 2 cut(s) 457, 523
MnlI CCTC 5 cut(s) 94, 331, 516, 590, 646
Mox20I TGGCCA 1 cut(s) 571
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 571
MseI TTAA 4 cut(s) 75, 125, 366, 711
Msp20I TGGCCA 1 cut(s) 571
MspA1I CMGCKG 1 cut(s) 518
MspCI CTTAAG 1 cut(s) 124
MspI CCGG 6 cut(s) 23, 83, 315, 456, 553, 631
MspR9I CCNGG 2 cut(s) 316, 456
MwoI GCNNNNNNNGC 3 cut(s) 230, 239, 677
NciI CCSGG 2 cut(s) 316, 456
NdeII GATC 2 cut(s) 64, 603
NlaIII CATG 7 cut(s) 32, 379, 430, 491, 496, 570, 709
NlaIV GGNNCC 4 cut(s) 34, 166, 199, 243
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 639
PinAI ACCGGT 2 cut(s) 22, 630
PkrI GCNGC 5 cut(s) 81, 226, 520, 523, 618
PshAI GACNNNNGTC 1 cut(s) 276
PspN4I GGNNCC 4 cut(s) 34, 166, 199, 243
PspOMI GGGCCC 1 cut(s) 197
PspPI GGNCC 3 cut(s) 164, 197, 198
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 64
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
SalI GTCGAC 1 cut(s) 430
SaqAI TTAA 4 cut(s) 75, 125, 366, 711
SatI GCNGC 5 cut(s) 80, 225, 519, 522, 617
Sau3AI GATC 2 cut(s) 64, 603
Sau96I GGNCC 3 cut(s) 164, 197, 198
ScrFI CCNGG 2 cut(s) 316, 456
SduI GDGCHC 4 cut(s) 123, 201, 244, 682
SetI ASST 7 cut(s) 81, 93, 98, 286, 520, 612, 657
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 716
SinI GGWCC 1 cut(s) 164
SmlI CTYRAG 2 cut(s) 124, 589
SmoI CTYRAG 2 cut(s) 124, 589
Sse9I AATT 2 cut(s) 249, 379
SsiI CCGC 4 cut(s) 62, 225, 459, 617
SspMI CTAG 2 cut(s) 281, 525
StyD4I CCNGG 2 cut(s) 314, 454
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 178, 502
TaiI ACGT 2 cut(s) 93, 612
TaqI TCGA 6 cut(s) 151, 326, 402, 431, 582, 606
TasI AATT 2 cut(s) 249, 379
TauI GCSGC 2 cut(s) 227, 619
TfiI GAWTC 1 cut(s) 639
Tru1I TTAA 4 cut(s) 75, 125, 366, 711
Tru9I TTAA 4 cut(s) 75, 125, 366, 711
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 3 cut(s) 79, 518, 521
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 5 cut(s) 17, 17, 324, 366, 392
TspRI CASTG 1 cut(s) 682
Vha464I CTTAAG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 281, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.