pycom01g20570

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020381
Physical Location & Seq
Forward (+)
18937704 .. 18938159
456 bp
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UTR
Exon/CDS
Intron
pycom01g20570.1

Sequence Viewer

Length: 456 bp
ATGGATGACAGGCGTTTAGCCATGGCAAAATCTCTCGGCGCCGATGGCACGGTCAAAGTTTCGACAAAAATGGAGGATTTAGATGACGAAGTTGCCAAGATTAAAGAAGCTATGGGATCCGAAGTTGATGTGACCTTCGACTGTGTAGGATTCAACAAAACCATGTCTACGGGCCTCAATGCCACTCGTCCCGGCGGCAAAGTTTGCCTTGTCGGAATGGGACACGGGGTGATGACAGTCCCTCTCACTCCCGCTGCTGCCAGGGAGGTTGATGTGGTTGGAGTTTTTCGTTACAAGAATACATGGCCGCTTTGCCTTGAGTTTTTGAGAAACGGGAAGATCAACGTGAAGCCGCTTATTACCCACCGGTTTGGTTTTACCGAGAAGGAGGTAGAAGAAGCTTTTGCAACCAGTGCTCGGGGGGGCTACGCCATTAAAGTCATGTTTACTCTATAG

Protein Analysis

152

Amino Acids

16.42

Weight (kDa)

7.7

Isoelectric Point (pI)

21.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 2 - 109 1.8e-21 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 12 - 141 1e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020381)

Species Orthologous Gene IDs
malus_domestica MD00G1006200.v1.1 MD01G1195100.v1.1
pyrus_communis pycom01g20520 pycom01g20570 pycom01g20590 pycom07g24120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 167
AciI CCGC 4 cut(s) 195, 252, 308, 353
AclWI GGATC 2 cut(s) 111, 124
AcoI YGGCCR 1 cut(s) 305
AcyI GRCGYC 1 cut(s) 39
AdeI CACNNNGTG 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 366
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 2 cut(s) 110, 401
AluI AGCT 2 cut(s) 110, 401
Alw21I GWGCWC 1 cut(s) 418
AlwI GGATC 2 cut(s) 111, 124
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 2 cut(s) 172, 305
ApeKI GCWGC 2 cut(s) 254, 257
AsiGI ACCGGT 1 cut(s) 366
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 172
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 417
BamHI GGATCC 1 cut(s) 116
BanI GGYRCC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 241, 244
BccI CCATC 1 cut(s) 38
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 1 cut(s) 192
BfmI CTRYAG 1 cut(s) 452
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 5 cut(s) 196, 255, 258, 308, 353
BlsI GCNGC 5 cut(s) 197, 256, 259, 309, 354
Bme1390I CCNGG 2 cut(s) 192, 262
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 2 cut(s) 40, 118
BmrFI CCNGG 2 cut(s) 192, 262
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 21, 261
BsaWI WCCGGW 1 cut(s) 366
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 2 cut(s) 21, 261
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 411
BseXI GCAGC 2 cut(s) 241, 244
BshFI GGCC 2 cut(s) 174, 307
BshNI GGYRCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 2 cut(s) 192, 367
BslFI GGGAC 3 cut(s) 174, 224, 234
BslI CCNNNNNNNGG 1 cut(s) 417
BsmFI GGGAC 3 cut(s) 174, 224, 234
BsnI GGCC 2 cut(s) 174, 307
BsoBI CYCGRG 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 2 cut(s) 116, 339
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 4 cut(s) 195, 252, 308, 353
BspANI GGCC 2 cut(s) 174, 307
BspLI GGNNCC 2 cut(s) 40, 118
BspPI GGATC 2 cut(s) 111, 124
BspT107I GGYRCC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 366
BsrI ACTGG 1 cut(s) 411
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 2 cut(s) 21, 261
BssMI GATC 2 cut(s) 116, 339
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 3 cut(s) 52, 143, 238
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 2 cut(s) 204, 413
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 2 cut(s) 119, 342
BstMBI GATC 2 cut(s) 116, 339
BstMWI GCNNNNNNNGC 3 cut(s) 45, 204, 413
BstNI CCWGG 1 cut(s) 262
BstSCI CCNGG 2 cut(s) 190, 260
BstSFI CTRYAG 1 cut(s) 452
BstV1I GCAGC 2 cut(s) 241, 244
BstX2I RGATCY 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 371
BstYI RGATCY 1 cut(s) 116
BsuRI GGCC 2 cut(s) 174, 307
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 418
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 172
CspAI ACCGGT 1 cut(s) 366
CviAII CATG 4 cut(s) 22, 163, 303, 442
CviJI RGCY 7 cut(s) 20, 110, 174, 307, 352, 401, 426
CviKI_1 RGCY 7 cut(s) 20, 110, 174, 307, 352, 401, 426
DinI GGCGCC 1 cut(s) 40
DpnI GATC 2 cut(s) 118, 341
DpnII GATC 2 cut(s) 116, 339
DraIII CACNNNGTG 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 417
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 4 cut(s) 25, 166, 306, 445
FaiI YATR 6 cut(s) 23, 113, 164, 304, 443, 454
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 3 cut(s) 174, 224, 234
FatI CATG 4 cut(s) 21, 162, 302, 441
FauI CCCGC 1 cut(s) 259
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 5 cut(s) 196, 255, 258, 308, 353
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 5 cut(s) 196, 255, 258, 308, 353
GlaI GCGC 1 cut(s) 40
GluI GCNGC 5 cut(s) 196, 255, 258, 308, 353
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 2 cut(s) 174, 307
HapII CCGG 2 cut(s) 192, 367
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 25, 166, 306, 445
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 1 cut(s) 150
HpaII CCGG 2 cut(s) 192, 367
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 2 cut(s) 168, 447
Hpy188I TCNGA 2 cut(s) 121, 215
Hpy8I GTNNAC 2 cut(s) 168, 447
HpyAV CCTTC 2 cut(s) 145, 379
HpyCH4III ACNGT 3 cut(s) 52, 143, 238
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 1 cut(s) 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 204, 413
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 25, 166, 306, 445
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 116, 339
LpnPI CCDG 5 cut(s) 205, 247, 274, 380, 424
Lsp1109I GCAGC 2 cut(s) 241, 244
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 130, 290
MalI GATC 2 cut(s) 118, 341
MboI GATC 2 cut(s) 116, 339
MboII GAAGA 2 cut(s) 349, 407
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 418
Mly113I GGCGCC 1 cut(s) 39
MmeI TCCRAC 2 cut(s) 193, 259
MnlI CCTC 5 cut(s) 67, 185, 252, 259, 382
MseI TTAA 2 cut(s) 102, 435
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 2 cut(s) 192, 367
MspR9I CCNGG 2 cut(s) 192, 262
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 3 cut(s) 45, 204, 413
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 1 cut(s) 192
NcoI CCATGG 1 cut(s) 21
NdeII GATC 2 cut(s) 116, 339
NlaIII CATG 4 cut(s) 25, 166, 306, 445
NlaIV GGNNCC 2 cut(s) 40, 118
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 150
PinAI ACCGGT 1 cut(s) 366
PkrI GCNGC 5 cut(s) 197, 256, 259, 309, 354
PluTI GGCGCC 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 40, 118
PspPI GGNCC 1 cut(s) 172
PsuI RGATCY 1 cut(s) 116
SaqAI TTAA 2 cut(s) 102, 435
SatI GCNGC 5 cut(s) 196, 255, 258, 308, 353
Sau3AI GATC 2 cut(s) 116, 339
Sau96I GGNCC 1 cut(s) 172
ScrFI CCNGG 2 cut(s) 192, 262
SduI GDGCHC 1 cut(s) 418
SetI ASST 6 cut(s) 112, 137, 270, 348, 393, 403
SfcI CTRYAG 1 cut(s) 452
SfoI GGCGCC 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SsiI CCGC 4 cut(s) 195, 252, 308, 353
SspDI GGCGCC 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 190, 260
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 3 cut(s) 52, 143, 238
TaiI ACGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 62, 138
TauI GCSGC 3 cut(s) 198, 310, 355
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 2 cut(s) 102, 435
Tru9I TTAA 2 cut(s) 102, 435
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 2 cut(s) 254, 257
Tsp45I GTSAC 1 cut(s) 130
TspRI CASTG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.