pycom01g20590

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020381
Physical Location & Seq
Forward (+)
18953273 .. 18953863
591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20590.1

Sequence Viewer

Length: 591 bp
ATGCATGTCGGCGAGCCAATGTTGGTCCCGAAACAACTGTTCTTAGTTGGATCGATCATCGGAGCAGGGCCTATTGGTCTGGTTTCAGTTTTGGCCGCTCGTGCTTTCGGGGCACCAAGAATTGTCATTGTGGATATGGATGACAAGCGTTTAGCCATGGCAAAGTCTGTCGGCGCTGATGGCACCGTCAAAGTTTCAACAAAAATGGAGGATTTGGATGACGAAGTTGCCAAGATTAAAGAAGCCATGGAATCCGAGGTGGATGTGACCTTTGATTGTGTGGGTTTTAATAAAACCATGTCGACGGGTCTCAACGCCACTCGTCCCGGCGGCAAAGTCTGCCTTGTCGGAATGGGACACGGCATGATGACAGTGCCTCTCACTCCCGCTGCTGCCAGGGAGGTTGATGTGGTTGGTGTTTTCCGGTACAAGAACACATGGCCACTTTGCCTCGATTTTTTGAGAAGTGGGAAGATCGACGTGAAACCGCTTATTACTCACCGGTTTGGATTTACCGAGAAGGAGGTGGAAGAAGCCTTTGCTACCAGTGCTCGTGGGGGTGACGCCATCAAAGTCATGTTTAATCTATAG

Protein Analysis

197

Amino Acids

21.0

Weight (kDa)

6.31

Isoelectric Point (pI)

22.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_dehyd_C PF16912 19 - 122 2.7e-07 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 24 - 154 1.9e-29 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 73 - 186 1.2e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020381)

Species Orthologous Gene IDs
malus_domestica MD00G1006200.v1.1 MD01G1195100.v1.1
pyrus_communis pycom01g20520 pycom01g20570 pycom01g20590 pycom07g24120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 112, 182
AccBSI CCGCTC 1 cut(s) 98
AccI GTMKAC 1 cut(s) 302
AciI CCGC 4 cut(s) 96, 330, 387, 488
AclWI GGATC 1 cut(s) 58
AcoI YGGCCR 2 cut(s) 93, 440
AcyI GRCGYC 1 cut(s) 564
AfaI GTAC 1 cut(s) 428
AgeI ACCGGT 1 cut(s) 501
AgsI TTSAA 1 cut(s) 198
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 1 cut(s) 395
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 1 cut(s) 58
AoxI GGCC 3 cut(s) 68, 93, 440
ApeKI GCWGC 2 cut(s) 389, 392
AsiGI ACCGGT 1 cut(s) 501
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 25, 68
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 491, 572
AvaII GGWCC 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 115
BalI TGGCCA 1 cut(s) 442
BanI GGYRCC 2 cut(s) 112, 182
BauI CACGAG 2 cut(s) 99, 552
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 2 cut(s) 376, 379
BccI CCATC 2 cut(s) 173, 575
BceAI ACGGC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 397
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 314
BfmI CTRYAG 1 cut(s) 587
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 4 cut(s) 96, 331, 390, 393
BlsI GCNGC 4 cut(s) 97, 332, 391, 394
Bme1390I CCNGG 2 cut(s) 327, 397
Bme18I GGWCC 1 cut(s) 25
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 2 cut(s) 25, 68
BmiI GGNNCC 3 cut(s) 27, 114, 184
BmrFI CCNGG 2 cut(s) 327, 397
BpuMI CCSGG 1 cut(s) 327
Bsa29I ATCGAT 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 4 cut(s) 156, 246, 255, 396
BsaWI WCCGGW 2 cut(s) 423, 501
Bse118I RCCGGY 1 cut(s) 501
Bse1I ACTGG 1 cut(s) 546
BseBI CCWGG 1 cut(s) 397
BseCI ATCGAT 1 cut(s) 53
BseDI CCNNGG 4 cut(s) 156, 246, 255, 396
BseGI GGATG 3 cut(s) 145, 223, 268
BseNI ACTGG 1 cut(s) 546
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 2 cut(s) 376, 379
BshFI GGCC 3 cut(s) 70, 95, 442
BshNI GGYRCC 2 cut(s) 112, 182
BshTI ACCGGT 1 cut(s) 501
BshVI ATCGAT 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 3 cut(s) 327, 424, 502
BslFI GGGAC 3 cut(s) 11, 309, 369
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 3 cut(s) 11, 309, 369
BsnI GGCC 3 cut(s) 70, 95, 442
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 115, 553
Bsp143I GATC 3 cut(s) 50, 54, 474
Bsp19I CCATGG 2 cut(s) 156, 246
BspACI CCGC 4 cut(s) 96, 330, 387, 488
BspANI GGCC 3 cut(s) 70, 95, 442
BspDI ATCGAT 1 cut(s) 53
BspLI GGNNCC 3 cut(s) 27, 114, 184
BspPI GGATC 1 cut(s) 58
BspT107I GGYRCC 2 cut(s) 112, 182
BspTNI GGTCTC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 501
BsrI ACTGG 1 cut(s) 546
BssAI RCCGGY 1 cut(s) 501
BssECI CCNNGG 4 cut(s) 156, 246, 255, 396
BssMI GATC 3 cut(s) 50, 54, 474
BssNI GRCGYC 1 cut(s) 564
BssSI CACGAG 2 cut(s) 99, 552
BssT1I CCWWGG 2 cut(s) 156, 246
Bst2BI CACGAG 2 cut(s) 99, 552
Bst2UI CCWGG 1 cut(s) 397
Bst4CI ACNGT 3 cut(s) 39, 187, 373
BstACI GRCGYC 1 cut(s) 564
BstAPI GCANNNNNTGC 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 14
BstDEI CTNAG 1 cut(s) 43
BstDSI CCRYGG 2 cut(s) 156, 246
BstF5I GGATG 3 cut(s) 145, 223, 268
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 3 cut(s) 53, 57, 477
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 3 cut(s) 50, 54, 474
BstMWI GCNNNNNNNGC 5 cut(s) 101, 110, 180, 339, 548
BstNI CCWGG 1 cut(s) 397
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 325, 395
BstSFI CTRYAG 1 cut(s) 587
BstSLI GKGCMC 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 376, 379
Bsu15I ATCGAT 1 cut(s) 53
BsuRI GGCC 3 cut(s) 70, 95, 442
BsuTUI ATCGAT 1 cut(s) 53
BtgI CCRYGG 2 cut(s) 156, 246
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 3 cut(s) 145, 223, 268
BtsIMutI CAGTG 2 cut(s) 378, 553
Cac8I GCNNGC 1 cut(s) 14
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 501
Cfr13I GGNCC 2 cut(s) 25, 68
ClaI ATCGAT 1 cut(s) 53
CseI GACGC 1 cut(s) 572
Csp6I GTAC 1 cut(s) 427
CspAI ACCGGT 1 cut(s) 501
CviAII CATG 7 cut(s) 5, 157, 247, 298, 364, 438, 577
CviJI RGCY 7 cut(s) 16, 70, 95, 155, 245, 442, 536
CviKI_1 RGCY 7 cut(s) 16, 70, 95, 155, 245, 442, 536
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 1 cut(s) 43
DpnI GATC 3 cut(s) 52, 56, 476
DpnII GATC 3 cut(s) 50, 54, 474
EaeI YGGCCR 2 cut(s) 93, 440
Eco130I CCWWGG 2 cut(s) 156, 246
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 395
EcoT14I CCWWGG 2 cut(s) 156, 246
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 156, 246
FaeI CATG 7 cut(s) 8, 160, 250, 301, 367, 441, 580
FaiI YATR 9 cut(s) 6, 137, 158, 248, 299, 365, 439, 578, 589
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 3 cut(s) 11, 309, 369
FatI CATG 7 cut(s) 4, 156, 246, 297, 363, 437, 576
FauI CCCGC 1 cut(s) 394
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 4 cut(s) 96, 331, 390, 393
FokI GGATG 3 cut(s) 152, 230, 275
Fsp4HI GCNGC 4 cut(s) 96, 331, 390, 393
GlaI GCGC 1 cut(s) 175
GluI GCNGC 4 cut(s) 96, 331, 390, 393
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 3 cut(s) 70, 95, 442
HapII CCGG 3 cut(s) 327, 424, 502
HgaI GACGC 1 cut(s) 572
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 1 cut(s) 564
Hin1II CATG 7 cut(s) 8, 160, 250, 301, 367, 441, 580
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HinfI GANTC 1 cut(s) 251
HpaII CCGG 3 cut(s) 327, 424, 502
HphI GGTGA 2 cut(s) 491, 572
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 3 cut(s) 62, 256, 350
Hpy188III TCNNGA 1 cut(s) 28
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 2 cut(s) 307, 482
HpyAV CCTTC 1 cut(s) 514
HpyCH4III ACNGT 3 cut(s) 39, 187, 373
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 110, 180, 339, 548
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 1 cut(s) 480
Hsp92I GRCGYC 1 cut(s) 564
Hsp92II CATG 7 cut(s) 8, 160, 250, 301, 367, 441, 580
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 3 cut(s) 50, 54, 474
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 8 cut(s) 51, 65, 340, 382, 409, 437, 515, 559
Lsp1109I GCAGC 2 cut(s) 376, 379
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 2 cut(s) 265, 560
MalI GATC 3 cut(s) 52, 56, 476
MbiI CCGCTC 1 cut(s) 98
MboI GATC 3 cut(s) 50, 54, 474
MboII GAAGA 2 cut(s) 484, 542
MhlI GDGCHC 2 cut(s) 115, 553
MlsI TGGCCA 1 cut(s) 442
MluCI AATT 1 cut(s) 120
MluNI TGGCCA 1 cut(s) 442
MmeI TCCRAC 2 cut(s) 28, 328
MnlI CCTC 6 cut(s) 202, 250, 387, 394, 461, 517
Mox20I TGGCCA 1 cut(s) 442
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 442
MseI TTAA 3 cut(s) 237, 288, 582
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 3 cut(s) 327, 424, 502
MspR9I CCNGG 2 cut(s) 327, 397
MvaI CCWGG 1 cut(s) 397
MwoI GCNNNNNNNGC 5 cut(s) 101, 110, 180, 339, 548
NciI CCSGG 1 cut(s) 327
NcoI CCATGG 2 cut(s) 156, 246
NdeII GATC 3 cut(s) 50, 54, 474
NlaIII CATG 7 cut(s) 8, 160, 250, 301, 367, 441, 580
NlaIV GGNNCC 3 cut(s) 27, 114, 184
NmuCI GTSAC 2 cut(s) 265, 560
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PfeI GAWTC 1 cut(s) 251
PinAI ACCGGT 1 cut(s) 501
PkrI GCNGC 4 cut(s) 97, 332, 391, 394
Psp6I CCWGG 1 cut(s) 395
PspGI CCWGG 1 cut(s) 395
PspN4I GGNNCC 3 cut(s) 27, 114, 184
PspPI GGNCC 2 cut(s) 25, 68
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
SalI GTCGAC 1 cut(s) 301
SaqAI TTAA 3 cut(s) 237, 288, 582
SatI GCNGC 4 cut(s) 96, 331, 390, 393
Sau3AI GATC 3 cut(s) 50, 54, 474
Sau96I GGNCC 2 cut(s) 25, 68
ScrFI CCNGG 2 cut(s) 327, 397
SduI GDGCHC 2 cut(s) 115, 553
SetI ASST 5 cut(s) 261, 272, 405, 483, 528
SfcI CTRYAG 1 cut(s) 587
SinI GGWCC 1 cut(s) 25
Sse9I AATT 1 cut(s) 120
SsiI CCGC 4 cut(s) 96, 330, 387, 488
StyD4I CCNGG 2 cut(s) 325, 395
StyI CCWWGG 2 cut(s) 156, 246
TaaI ACNGT 3 cut(s) 39, 187, 373
TaiI ACGT 1 cut(s) 483
TaqI TCGA 4 cut(s) 53, 302, 453, 477
TasI AATT 1 cut(s) 120
TauI GCSGC 2 cut(s) 98, 333
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 3 cut(s) 237, 288, 582
Tru9I TTAA 3 cut(s) 237, 288, 582
TscAI CASTG 2 cut(s) 378, 553
TseFI GTSAC 2 cut(s) 265, 560
TseI GCWGC 2 cut(s) 389, 392
Tsp45I GTSAC 2 cut(s) 265, 560
TspRI CASTG 2 cut(s) 378, 553
VpaK11BI GGWCC 1 cut(s) 25
XceI RCATGY 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.