Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004675

Physical Location & Seq

Genomic Coordinates Reverse (-)

12851634 .. 12853756

Gene Structure 2123 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1066600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGCTGCCACCACCAACCCCTCTTCCTCCACACTCTCTTCCTCTCTGTCCAATCCCTCTCCTCTCCCCCGAACCCACAAAACCCCTCCCTCCTTCTCCTCCAAAACCCTCCCTAAACCCGCTTTCCCTCTCAAAACCTCCTCCCTTTCACATACCCCCAAGCCCTCCTCCTTCACCCTCCACTCTAAAAACCCAATTTCCGACGTTTTCTCGGCCGCCAAACAAAACACAGACGGCAATTCAATCGATTTTGTTGATGACGAAAAGCCCCGGGAGGAGTGTGGCGTGGTCGGCATATACGGCGACCCGGAGGCCTCCCGTCTTTGCTATTTGGCCCTGCACGCGCTCCAGCATCGCGGTCAGGAAGGTGCCGGGATCGTGTGTGTGAAAGACGACGTGCTTCAGTCTATAACAGGCGTGGGGCTCGTCTCCGAGGTCTTCAATCAGACCAAGCTTGACCAGCTGCCCGGCGACTTGGCAATTGGGCATGTCAGGTACTCCACTGCAGGCTCCTCCATGCTAAAAAATGTACAGCCTTTTGTTGCAGGGTACAGGTTTGGCTCAGTTGGGGTTGCCCACAATGGCAATTTGGTAAACTACCGCTCCCTTCGAGCTAAGCTCGAAGACAACGGCTCCATTTTCAACACCAGCTCTGACACAGAGGTCGTTCTTCACCTTATAGCAATTTCTAAGCATAGACCTTTTATTTTGAGAATTGTTGATGCATGCGAGCAGATTGAGGGGGCTTATTCTATGGTTTTCGTGACCAAAGATAAGCTCGTGGCGGTTCGGGACCCTCACGGGTTTCGGCCATTGGTAATGGGTAGGAGGAGCAATGGCTCTGTTGTGTTTGCTTCTGAGACTTGTGCTCTTGATTTGATTGAAGCCACATATGAGAGAGAGGTGTTTCCTGGAGAGGTTGTGGTGGTGGATAAAGAGGGGGTTCAATCACTTTGCCTAATGTCTCATCCTCAGCCAAAGCAATGTATCTTTGAGCACATTTACTTTGCTCTTCCGAATTCTGTGGTTTTCGGTCGTTCAGTGTATGAGTCCCGACGGGCATTTGGTGAGATACTTGCCACTGAGGCGCCGGTTGATTGTGATGTTGTGATAGCTGTGCCTGATTCTGGGGTGGTAGCCGCACTTGGCTATGCTGCTAAAGCTGGTGTGGCTTTTCAGCAGGGTTTGATTAGGTCTCACTATGTCGGGAGGACCTTCATTGAGCCATCACAGAAGATTAGGGACTTTGGTGTGAAGCTCAAGTTGTCCCCGGTTCGTGCGGTGTTGGAGGGGAAGAGGGTTGTGGTTGTGGATGACTCCATTGTGAGAGGAACCACTTCTTCGAAAATTGTTAGGTTGTTGAAGGAGGCTGGTGCAAAGGAAGTTCATATGAGGATTGCAAGCCCGCCGATTATTGGTTCTTGTTATTACGGAGTGGACACACCAAGCTCTGAGGAGTTGATTTCAAATAGGATGAGTGTTGAGGAAATTAGGGAGTTTATCGGATCGGATTCACTTGCTTTTCTGCCAATTGGTAAATTGGAGAAAATGTTGGGCAGTCAATCTCCTAACTTTTGTTATGCTTGCTTCTCTGGAAAATACCCCGTGGAGCCTAGAGAGATTAAAGTGAAACGAGTGGGTGATTCCTTGGATGATGGGTTAGTTGGTAGTTTTGAGAACATTGATGGTGGCTGGGTTCAAGCGAATCAGAGCCCAAAAGAGGAGAAGGACAAGGAATTTGAGGGCAGTGTTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004044 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006144 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009112 GO:0009113 GO:0009117 GO:0009165 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0016740 GO:0016757 GO:0016763 GO:0018130 GO:0019438 GO:0019637 GO:0030312 GO:0034641 GO:0034654 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046112 GO:0046148 GO:0046483 GO:0055086 GO:0071704 GO:0071840 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00250 ko01100 ko01110 ko01130 map00230 map00250 map01100 map01110 map01130

Pfam Domains

Protein Families

GATase_7 Pribosyltran

Protein Analysis

586

Amino Acids

63.47

Weight (kDa)

6.42

Isoelectric Point (pI)

47.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	157 - 287	5.2e-20	Glutamine amidotransferase domain
GATase_7	PF13537	174 - 291	4.4e-22	Glutamine amidotransferase domain
Pribosyltran	PF00156	351 - 463	3.4e-09	Phosphoribosyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004675)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16570 AT4G34740 AT4G38880
fragaria_vesca	FvH4_1g25950 FvH4_7g05510
malus_domestica	MD00G1066600.v1.1 MD10G1061900.v1.1
prunus_persica	Prupe.8G082700_v2.0.a1
pyrus_communis	pycom05g04470 pycom10g04760
rosa_chinensis	RchiOBHm_Chr6g0268481 RchiOBHm_Chr6g0268491
rosa_laevigata	RLG00000013908 RLG00000013909
rosa_multiflora	Rmu_co8446293.1_g000001 Rmu_sc0002404.1_g000018 Rmu_sc0002404.1_g000024 Rmu_sc0008184.1_g000006
rosa_roxburghii	Rroxscaffold_7G00199790 Rroxscaffold_7G00199800
rosa_rugosa	Rorug06G0042200 Rorug06G0042300
rosa_samantha	Rh6AG161600 Rh6AG161900 Rh6BG165900 Rh6CG159500 Rh6CG159700 Rh6DG151500 Rh6DG151600
rosa_wichuraiana	Rw6G013970 Rw6G013980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	662
AccB1I	GGYRCC	2 cut(s)	368, 1087
AccBSI	CCGCTC	1 cut(s)	603
AccII	CGCG	2 cut(s)	344, 357
AciI	CCGC	8 cut(s)	120, 216, 357, 601, 785, 1140, 1280, 1406
AclWI	GGATC	2 cut(s)	383, 1513
AcoI	YGGCCR	2 cut(s)	213, 809
AcsI	RAATTY	2 cut(s)	1018, 1736
AcuI	CTGAAG	1 cut(s)	386
AcyI	GRCGYC	1 cut(s)	1088
AfaI	GTAC	3 cut(s)	497, 531, 551
AfiI	CCNNNNNNNGG	3 cut(s)	1127, 1415, 1720
AgsI	TTSAA	8 cut(s)	243, 442, 643, 884, 947, 1363, 1467, 1700
AjiI	CACGTC	1 cut(s)	397
AjnI	CCWGG	1 cut(s)	910
Alw21I	GWGCWC	2 cut(s)	871, 999
Alw26I	GTCTC	4 cut(s)	433, 854, 969, 1200
AlwI	GGATC	2 cut(s)	383, 1513
Ama87I	CYCGRG	1 cut(s)	270
AoxI	GGCC	4 cut(s)	213, 312, 333, 809
ApeKI	GCWGC	3 cut(s)	5, 463, 1154
ApoI	RAATTY	2 cut(s)	1018, 1736
ArsI	GACNNNNNNTTYG	2 cut(s)	1721, 1753
Asp700I	GAANNNNTTC	1 cut(s)	1336
AspLEI	GCGC	2 cut(s)	346, 1090
AspS9I	GGNCC	3 cut(s)	334, 793, 1212
AsuC2I	CCSGG	6 cut(s)	271, 272, 308, 373, 468, 1271
AsuHPI	GGTGA	4 cut(s)	166, 665, 1079, 1652
AsuII	TTCGAA	1 cut(s)	1343
AvaI	CYCGRG	1 cut(s)	270
AvaII	GGWCC	2 cut(s)	793, 1212
BanI	GGYRCC	2 cut(s)	368, 1087
BanII	GRGCYC	2 cut(s)	426, 1715
BauI	CACGAG	1 cut(s)	779
BbsI	GAAGAC	2 cut(s)	430, 630
Bbv12I	GWGCWC	2 cut(s)	871, 999
BbvCI	CCTCAGC	1 cut(s)	972
BbvI	GCAGC	2 cut(s)	450, 1141
BccI	CCATC	3 cut(s)	1234, 1649, 1679
BceAI	ACGGC	3 cut(s)	250, 316, 646
BcgI	CGANNNNNNTGC	2 cut(s)	226, 260
BciT130I	CCWGG	1 cut(s)	912
BcnI	CCSGG	6 cut(s)	271, 272, 308, 373, 468, 1271
BcoDI	GTCTC	4 cut(s)	433, 854, 969, 1200
BfaI	CTAG	1 cut(s)	1614
BfmI	CTRYAG	1 cut(s)	504
BfoI	RGCGCY	1 cut(s)	1091
BglI	GCCNNNNNGGC	1 cut(s)	1085
BisI	GCNGC	5 cut(s)	6, 216, 464, 1140, 1155
BlpI	GCTNAGC	1 cut(s)	615
BlsI	GCNGC	5 cut(s)	7, 217, 465, 1141, 1156
Bme1390I	CCNGG	7 cut(s)	271, 272, 308, 373, 468, 912, 1271
Bme18I	GGWCC	2 cut(s)	793, 1212
BmeT110I	CYCGRG	1 cut(s)	270
BmgBI	CACGTC	1 cut(s)	397
BmgT120I	GGNCC	3 cut(s)	334, 793, 1212
BmiI	GGNNCC	8 cut(s)	370, 511, 634, 794, 795, 1089, 1333, 1611
BmrFI	CCNGG	7 cut(s)	271, 272, 308, 373, 468, 912, 1271
BmsI	GCATC	2 cut(s)	361, 712
BpiI	GAAGAC	2 cut(s)	430, 630
BplI	GAGNNNNNCTC	2 cut(s)	603, 635
BpmI	CTGGAG	2 cut(s)	332, 933
Bpu10I	CCTNAGC	1 cut(s)	972
Bpu1102I	GCTNAGC	1 cut(s)	615
Bpu14I	TTCGAA	1 cut(s)	1343
BpuEI	CTTGAG	1 cut(s)	1244
BpuMI	CCSGG	6 cut(s)	271, 272, 308, 373, 468, 1271
Bsa29I	ATCGAT	1 cut(s)	246
BsaHI	GRCGYC	1 cut(s)	1088
BsaI	GGTCTC	1 cut(s)	1200
BsaJI	CCNNGG	6 cut(s)	269, 270, 432, 1269, 1605, 1647
BsaXI	ACNNNNNCTCC	8 cut(s)	587, 617, 617, 647, 906, 936, 1535, 1565
Bsc4I	CCNNNNNNNGG	3 cut(s)	1127, 1415, 1720
Bse118I	RCCGGY	1 cut(s)	1090
Bse3DI	GCAATG	2 cut(s)	841, 989
BseBI	CCWGG	1 cut(s)	912
BseCI	ATCGAT	1 cut(s)	246
BseDI	CCNNGG	6 cut(s)	269, 270, 432, 1269, 1605, 1647
BseGI	GGATG	4 cut(s)	967, 1318, 1479, 1657
BseLI	CCNNNNNNNGG	3 cut(s)	1127, 1415, 1720
BseMI	GCAATG	2 cut(s)	841, 989
BseMII	CTCAG	5 cut(s)	576, 849, 986, 1074, 1443
BseRI	GAGGAG	9 cut(s)	51, 88, 130, 157, 290, 502, 844, 1469, 1736
BseX3I	CGGCCG	1 cut(s)	213
BseXI	GCAGC	2 cut(s)	450, 1141
BseYI	CCCAGC	1 cut(s)	1692
BsgI	GTGCAG	1 cut(s)	323
Bsh1236I	CGCG	2 cut(s)	344, 357
Bsh1285I	CGRYCG	2 cut(s)	216, 1036
BshFI	GGCC	4 cut(s)	215, 314, 335, 811
BshNI	GGYRCC	2 cut(s)	368, 1087
BshVI	ATCGAT	1 cut(s)	246
BsiEI	CGRYCG	2 cut(s)	216, 1036
BsiHKAI	GWGCWC	2 cut(s)	871, 999
BsiHKCI	CYCGRG	1 cut(s)	270
BsiSI	CCGG	6 cut(s)	271, 308, 372, 468, 1091, 1271
BslFI	GGGAC	4 cut(s)	806, 1036, 1252, 1256
BslI	CCNNNNNNNGG	3 cut(s)	1127, 1415, 1720
BsmAI	GTCTC	4 cut(s)	433, 854, 969, 1200
BsmBI	CGTCTC	1 cut(s)	433
BsmFI	GGGAC	4 cut(s)	806, 1036, 1252, 1256
BsnI	GGCC	4 cut(s)	215, 314, 335, 811
Bso31I	GGTCTC	1 cut(s)	1200
BsoBI	CYCGRG	1 cut(s)	270
Bsp119I	TTCGAA	1 cut(s)	1343
Bsp1286I	GDGCHC	4 cut(s)	426, 871, 999, 1715
Bsp1407I	TGTACA	1 cut(s)	529
Bsp143I	GATC	2 cut(s)	375, 1505
Bsp1720I	GCTNAGC	1 cut(s)	615
BspACI	CCGC	8 cut(s)	120, 216, 357, 601, 785, 1140, 1280, 1406
BspANI	GGCC	4 cut(s)	215, 314, 335, 811
BspCNI	CTCAG	5 cut(s)	575, 850, 985, 1075, 1444
BspDI	ATCGAT	1 cut(s)	246
BspFNI	CGCG	2 cut(s)	344, 357
BspLI	GGNNCC	8 cut(s)	370, 511, 634, 794, 795, 1089, 1333, 1611
BspMAI	CTGCAG	1 cut(s)	508
BspPI	GGATC	2 cut(s)	383, 1513
BspQI	GCTCTTC	1 cut(s)	1017
BspT104I	TTCGAA	1 cut(s)	1343
BspT107I	GGYRCC	2 cut(s)	368, 1087
BspTNI	GGTCTC	1 cut(s)	1200
BsrBI	CCGCTC	1 cut(s)	603
BsrDI	GCAATG	2 cut(s)	841, 989
BsrFI	RCCGGY	1 cut(s)	1090
BsrGI	TGTACA	1 cut(s)	529
BssAI	RCCGGY	1 cut(s)	1090
BssECI	CCNNGG	6 cut(s)	269, 270, 432, 1269, 1605, 1647
BssMI	GATC	2 cut(s)	375, 1505
BssNI	GRCGYC	1 cut(s)	1088
BssSI	CACGAG	1 cut(s)	779
BssT1I	CCWWGG	1 cut(s)	1647
Bst2BI	CACGAG	1 cut(s)	779
Bst2UI	CCWGG	1 cut(s)	912
Bst6I	CTCTTC	4 cut(s)	28, 43, 1017, 1289
BstACI	GRCGYC	1 cut(s)	1088
BstAUI	TGTACA	1 cut(s)	529
BstBI	TTCGAA	1 cut(s)	1343
BstC8I	GCNNGC	7 cut(s)	342, 508, 727, 731, 1402, 1406, 1585
BstDEI	CTNAG	7 cut(s)	562, 615, 690, 858, 972, 1083, 1452
BstDSI	CCRYGG	1 cut(s)	1605
BstF5I	GGATG	4 cut(s)	967, 1318, 1479, 1657
BstFNI	CGCG	2 cut(s)	344, 357
BstH2I	RGCGCY	1 cut(s)	1091
BstHHI	GCGC	2 cut(s)	346, 1090
BstKTI	GATC	2 cut(s)	378, 1508
BstMAI	GTCTC	4 cut(s)	433, 854, 969, 1200
BstMBI	GATC	2 cut(s)	375, 1505
BstMCI	CGRYCG	2 cut(s)	216, 1036
BstMWI	GCNNNNNNNGC	7 cut(s)	291, 300, 341, 460, 1085, 1160, 1169
BstNI	CCWGG	1 cut(s)	912
BstNSI	RCATGY	2 cut(s)	491, 729
BstSCI	CCNGG	7 cut(s)	269, 270, 306, 371, 466, 910, 1269
BstSFI	CTRYAG	1 cut(s)	504
BstUI	CGCG	2 cut(s)	344, 357
BstV1I	GCAGC	2 cut(s)	450, 1141
BstV2I	GAAGAC	2 cut(s)	430, 630
BstZI	CGGCCG	1 cut(s)	213
Bsu15I	ATCGAT	1 cut(s)	246
BsuRI	GGCC	4 cut(s)	215, 314, 335, 811
BsuTUI	ATCGAT	1 cut(s)	246
BtgI	CCRYGG	1 cut(s)	1605
BtgZI	GCGATG	1 cut(s)	338
BtrI	CACGTC	1 cut(s)	397
BtsCI	GGATG	4 cut(s)	967, 1318, 1479, 1657
BtsI	GCAGTG	2 cut(s)	501, 1753
BtsIMutI	CAGTG	4 cut(s)	501, 1047, 1080, 1753
Cac8I	GCNNGC	7 cut(s)	342, 508, 727, 731, 1402, 1406, 1585
CfoI	GCGC	2 cut(s)	346, 1090
Cfr10I	RCCGGY	1 cut(s)	1090
Cfr13I	GGNCC	3 cut(s)	334, 793, 1212
Cfr9I	CCCGGG	1 cut(s)	270
ClaI	ATCGAT	1 cut(s)	246
Csp6I	GTAC	3 cut(s)	496, 530, 550
CviAII	CATG	3 cut(s)	488, 517, 726
CviQI	GTAC	3 cut(s)	496, 530, 550
DdeI	CTNAG	7 cut(s)	562, 615, 690, 858, 972, 1083, 1452
DinI	GGCGCC	1 cut(s)	1089
DpnI	GATC	2 cut(s)	377, 1507
DpnII	GATC	2 cut(s)	375, 1505
DrdI	GACNNNNNNGTC	1 cut(s)	662
DseDI	GACNNNNNNGTC	1 cut(s)	662
EaeI	YGGCCR	2 cut(s)	213, 809
EagI	CGGCCG	1 cut(s)	213
Eam1104I	CTCTTC	4 cut(s)	28, 43, 1017, 1289
EarI	CTCTTC	4 cut(s)	28, 43, 1017, 1289
EclXI	CGGCCG	1 cut(s)	213
Eco130I	CCWWGG	1 cut(s)	1647
Eco147I	AGGCCT	1 cut(s)	314
Eco24I	GRGCYC	2 cut(s)	426, 1715
Eco31I	GGTCTC	1 cut(s)	1200
Eco47I	GGWCC	2 cut(s)	793, 1212
Eco52I	CGGCCG	1 cut(s)	213
Eco57I	CTGAAG	1 cut(s)	386
Eco88I	CYCGRG	1 cut(s)	270
EcoO109I	RGGNCCY	2 cut(s)	793, 1212
EcoRI	GAATTC	1 cut(s)	1018
EcoRII	CCWGG	1 cut(s)	910
EcoT14I	CCWWGG	1 cut(s)	1647
EcoT22I	ATGCAT	1 cut(s)	727
EcoT38I	GRGCYC	2 cut(s)	426, 1715
EgeI	GGCGCC	1 cut(s)	1089
EheI	GGCGCC	1 cut(s)	1089
ErhI	CCWWGG	1 cut(s)	1647
Esp3I	CGTCTC	1 cut(s)	433
FaeI	CATG	3 cut(s)	491, 520, 729
FaqI	GGGAC	4 cut(s)	806, 1036, 1252, 1256
FatI	CATG	3 cut(s)	487, 516, 725
FauI	CCCGC	2 cut(s)	127, 1413
FauNDI	CATATG	2 cut(s)	892, 1389
Fnu4HI	GCNGC	5 cut(s)	6, 216, 464, 1140, 1155
FokI	GGATG	4 cut(s)	954, 1325, 1486, 1664
FriOI	GRGCYC	2 cut(s)	426, 1715
Fsp4HI	GCNGC	5 cut(s)	6, 216, 464, 1140, 1155
FspBI	CTAG	1 cut(s)	1614
GlaI	GCGC	2 cut(s)	345, 1089
GluI	GCNGC	5 cut(s)	6, 216, 464, 1140, 1155
GsaI	CCCAGC	1 cut(s)	1696
GsuI	CTGGAG	2 cut(s)	332, 933
HaeII	RGCGCY	1 cut(s)	1091
HaeIII	GGCC	4 cut(s)	215, 314, 335, 811
HapII	CCGG	6 cut(s)	271, 308, 372, 468, 1091, 1271
HhaI	GCGC	2 cut(s)	346, 1090
Hin1I	GRCGYC	1 cut(s)	1088
Hin1II	CATG	3 cut(s)	491, 520, 729
Hin6I	GCGC	2 cut(s)	344, 1088
HinP1I	GCGC	2 cut(s)	344, 1088
HindIII	AAGCTT	1 cut(s)	452
HinfI	GANTC	6 cut(s)	1049, 1124, 1316, 1511, 1643, 1705
HpaII	CCGG	6 cut(s)	271, 308, 372, 468, 1091, 1271
HphI	GGTGA	4 cut(s)	166, 665, 1079, 1652
Hpy166II	GTNNAC	2 cut(s)	595, 1438
Hpy188III	TCNNGA	7 cut(s)	362, 763, 791, 872, 1053, 1207, 1593
Hpy8I	GTNNAC	2 cut(s)	595, 1438
Hpy99I	CGWCG	3 cut(s)	206, 398, 1059
HpyAV	CCTTC	7 cut(s)	103, 181, 359, 617, 1225, 1357, 1720
HpyCH4IV	ACGT	2 cut(s)	204, 396
HpyCH4V	TGCA	6 cut(s)	340, 506, 545, 725, 1376, 1400
HpyF10VI	GCNNNNNNNGC	7 cut(s)	291, 300, 341, 460, 1085, 1160, 1169
HpyF3I	CTNAG	7 cut(s)	562, 615, 690, 858, 972, 1083, 1452
HpySE526I	ACGT	2 cut(s)	204, 396
Hsp92I	GRCGYC	1 cut(s)	1088
Hsp92II	CATG	3 cut(s)	491, 520, 729
HspAI	GCGC	2 cut(s)	344, 1088
KasI	GGCGCC	1 cut(s)	1087
KflI	GGGWCCC	1 cut(s)	793
Kzo9I	GATC	2 cut(s)	375, 1505
LguI	GCTCTTC	1 cut(s)	1017
LmnI	GCTCC	6 cut(s)	351, 515, 608, 638, 831, 1609
Lsp1109I	GCAGC	2 cut(s)	450, 1141
LweI	GCATC	2 cut(s)	361, 712
MaeI	CTAG	1 cut(s)	1614
MaeII	ACGT	2 cut(s)	204, 396
MaeIII	GTNAC	1 cut(s)	763
MalI	GATC	2 cut(s)	377, 1507
MbiI	CCGCTC	1 cut(s)	603
MboI	GATC	2 cut(s)	375, 1505
MboII	GAAGA	9 cut(s)	15, 30, 430, 635, 662, 1004, 1246, 1306, 1332
MfeI	CAATTG	2 cut(s)	480, 1530
MhlI	GDGCHC	4 cut(s)	426, 871, 999, 1715
Mly113I	GGCGCC	1 cut(s)	1088
MlyI	GAGTC	2 cut(s)	1058, 1310
MmeI	TCCRAC	2 cut(s)	225, 1266
Mph1103I	ATGCAT	1 cut(s)	727
MroXI	GAANNNNTTC	1 cut(s)	1336
MseI	TTAA	1 cut(s)	1623
MspA1I	CMGCKG	1 cut(s)	463
MspI	CCGG	6 cut(s)	271, 308, 372, 468, 1091, 1271
MspR9I	CCNGG	7 cut(s)	271, 272, 308, 373, 468, 912, 1271
MunI	CAATTG	2 cut(s)	480, 1530
MvaI	CCWGG	1 cut(s)	912
MvnI	CGCG	2 cut(s)	344, 357
MwoI	GCNNNNNNNGC	7 cut(s)	291, 300, 341, 460, 1085, 1160, 1169
NarI	GGCGCC	1 cut(s)	1088
NciI	CCSGG	6 cut(s)	271, 272, 308, 373, 468, 1271
NdeI	CATATG	2 cut(s)	892, 1389
NdeII	GATC	2 cut(s)	375, 1505
NlaIII	CATG	3 cut(s)	491, 520, 729
NlaIV	GGNNCC	8 cut(s)	370, 511, 634, 794, 795, 1089, 1333, 1611
NmeAIII	GCCGAG	1 cut(s)	191
NmuCI	GTSAC	1 cut(s)	763
NsiI	ATGCAT	1 cut(s)	727
NspI	RCATGY	2 cut(s)	491, 729
NspV	TTCGAA	1 cut(s)	1343
PaeI	GCATGC	1 cut(s)	729
PceI	AGGCCT	1 cut(s)	314
PciSI	GCTCTTC	1 cut(s)	1017
PdmI	GAANNNNTTC	1 cut(s)	1336
PfeI	GAWTC	4 cut(s)	1124, 1511, 1643, 1705
PfoI	TCCNGGA	1 cut(s)	910
PkrI	GCNGC	5 cut(s)	7, 217, 465, 1141, 1156
PleI	GAGTC	2 cut(s)	1057, 1310
PluTI	GGCGCC	1 cut(s)	1091
PpsI	GAGTC	2 cut(s)	1057, 1310
PpuMI	RGGWCCY	2 cut(s)	793, 1212
Psp5II	RGGWCCY	2 cut(s)	793, 1212
Psp6I	CCWGG	1 cut(s)	910
PspFI	CCCAGC	1 cut(s)	1692
PspGI	CCWGG	1 cut(s)	910
PspN4I	GGNNCC	8 cut(s)	370, 511, 634, 794, 795, 1089, 1333, 1611
PspPI	GGNCC	3 cut(s)	334, 793, 1212
PspPPI	RGGWCCY	2 cut(s)	793, 1212
PstI	CTGCAG	1 cut(s)	508
PvuII	CAGCTG	1 cut(s)	463
RsaI	GTAC	3 cut(s)	497, 531, 551
RsaNI	GTAC	3 cut(s)	496, 530, 550
SapI	GCTCTTC	1 cut(s)	1017
SaqAI	TTAA	1 cut(s)	1623
SatI	GCNGC	5 cut(s)	6, 216, 464, 1140, 1155
Sau3AI	GATC	2 cut(s)	375, 1505
Sau96I	GGNCC	3 cut(s)	334, 793, 1212
SchI	GAGTC	2 cut(s)	1058, 1310
ScrFI	CCNGG	7 cut(s)	271, 272, 308, 373, 468, 912, 1271
SduI	GDGCHC	4 cut(s)	426, 871, 999, 1715
SfaNI	GCATC	2 cut(s)	361, 712
SfcI	CTRYAG	1 cut(s)	504
SfoI	GGCGCC	1 cut(s)	1089
SfuI	TTCGAA	1 cut(s)	1343
SinI	GGWCC	2 cut(s)	793, 1212
SmaI	CCCGGG	1 cut(s)	272
SmlI	CTYRAG	1 cut(s)	1259
SmoI	CTYRAG	1 cut(s)	1259
SphI	GCATGC	1 cut(s)	729
SseBI	AGGCCT	1 cut(s)	314
SsiI	CCGC	8 cut(s)	120, 216, 357, 601, 785, 1140, 1280, 1406
SspDI	GGCGCC	1 cut(s)	1087
SspMI	CTAG	1 cut(s)	1614
StuI	AGGCCT	1 cut(s)	314
StyD4I	CCNGG	7 cut(s)	269, 270, 306, 371, 466, 910, 1269
StyI	CCWWGG	1 cut(s)	1647
TaiI	ACGT	2 cut(s)	207, 399
TaqI	TCGA	4 cut(s)	246, 610, 621, 1343
TaqII	GACCGA	1 cut(s)	1022
TatI	WGTACW	1 cut(s)	529
TauI	GCSGC	2 cut(s)	218, 1142
TfiI	GAWTC	4 cut(s)	1124, 1511, 1643, 1705
Tru1I	TTAA	1 cut(s)	1623
Tru9I	TTAA	1 cut(s)	1623
TscAI	CASTG	4 cut(s)	508, 1047, 1087, 1753
TseFI	GTSAC	1 cut(s)	763
TseI	GCWGC	3 cut(s)	5, 463, 1154
Tsp45I	GTSAC	1 cut(s)	763
TspDTI	ATGAA	2 cut(s)	1207, 1376
TspGWI	ACGGA	1 cut(s)	1446
TspMI	CCCGGG	1 cut(s)	270
TspRI	CASTG	4 cut(s)	508, 1047, 1087, 1753
VpaK11BI	GGWCC	2 cut(s)	793, 1212
XapI	RAATTY	2 cut(s)	1018, 1736
XceI	RCATGY	2 cut(s)	491, 729
XmaI	CCCGGG	1 cut(s)	270
XmnI	GAANNNNTTC	1 cut(s)	1336
XspI	CTAG	1 cut(s)	1614
Zsp2I	ATGCAT	1 cut(s)	727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.