MD00G1066800.v1.1

ribosome-binding factor A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012528
Physical Location & Seq
Reverse (-)
12880382 .. 12883822
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1066800.v1.1.491

Sequence Viewer

Length: 675 bp
ATGCCAAACTTGGTCCTCCACCGCCCACCACTCACCCCCTTCCTTTCCCACCTCCCATCATCATCATCATCATCATCATCAAGCTTTCCAATTCGGACCCAGAAATCAACGGCCTCGTTTCCCCCAACAAATCATGCGGTTCTGCTCCGTTGGCGGCCCAACACCATCAGGTGCATGGCGAACCCGCGCAGAGTGGCAATGGTGGCTAAGCAGATAAGGAGGGAGCTGTCTGACATGCTCTTAACTGATAAGGTCTTGCAGTACGCTATTCTTCCTGAGGTTTCTTTGGGTGCTGACCGTTACCTCTCTTCCCTAACCACCATAAGCGATGTTGAAGTCTCCACCGACTTGCAGGTGGTTAAAGTCTATGTTTCTATTTTTGGCGATGAAAGAGGGAAGGAGGTTGCCCTTGCTGGGCTAAAATCAAAAGCCAAATATGTCCGAGGTCAATTAGGAAGGCGTATGAAGCTGCGGTTAACTCCTGAGATACGCTTCATAGAAGATGAGTCTCTAGAGAGAGGAAGCAGGGTGATTGCTATATTAGATAAGATAAAGAATGAAAAGAAAGATTCAGAAATTCAAGATGACGAACAATTGGAACCATCCGAGGCACCTGAGGATGACGGAGATTGGGAGGGTGATGACCCTGATGAAGACATTATATACATAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009536 GO:0022613 GO:0042254 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RBFA

Protein Analysis

225

Amino Acids

25.28

Weight (kDa)

5.75

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBFA PF02033 64 - 180 3.5e-26 Ribosome-binding factor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34730 AT4G34730 AT4G34730
fragaria_vesca FvH4_2g10740 FvH4_2g10740
malus_domestica MD00G1066800.v1.1
prunus_persica Prupe.8G082300_v2.0.a1
pyrus_communis pycom05g04460 pycom10g04740
rosa_chinensis RchiOBHm_Chr6g0268531
rosa_laevigata RLG00000013905
rosa_multiflora Rmu_sc0012389.1_g000002
rosa_roxburghii Rroxscaffold_7G00199750
rosa_rugosa Rorug06G0042900
rosa_samantha Rh6AG162300 Rh6BG166500 Rh6CG160300 Rh6DG151700
rosa_wichuraiana Rw6G014010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 610
AccII CGCG 1 cut(s) 187
AciI CCGC 5 cut(s) 22, 137, 154, 185, 472
AcsI RAATTY 1 cut(s) 576
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 414
AgsI TTSAA 2 cut(s) 335, 581
AluBI AGCT 3 cut(s) 84, 226, 469
AluI AGCT 3 cut(s) 84, 226, 469
Alw26I GTCTC 2 cut(s) 343, 513
AoxI GGCC 2 cut(s) 111, 155
ApeKI GCWGC 1 cut(s) 469
ApoI RAATTY 1 cut(s) 576
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 3 cut(s) 13, 96, 156
AsuHPI GGTGA 3 cut(s) 25, 541, 650
AvaII GGWCC 2 cut(s) 13, 96
AxyI CCTNAGG 2 cut(s) 276, 615
BanI GGYRCC 1 cut(s) 610
BbsI GAAGAC 1 cut(s) 660
BbvI GCAGC 1 cut(s) 456
BccI CCATC 3 cut(s) 64, 173, 610
BceAI ACGGC 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 343, 513
BfaI CTAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 343
BisI GCNGC 2 cut(s) 155, 470
BlpI GCTNAGC 1 cut(s) 207
BlsI GCNGC 2 cut(s) 156, 471
Bme18I GGWCC 2 cut(s) 13, 96
BmgT120I GGNCC 3 cut(s) 13, 96, 156
BmiI GGNNCC 3 cut(s) 98, 600, 612
BpiI GAAGAC 1 cut(s) 660
Bpu1102I GCTNAGC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 442, 606
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse21I CCTNAGG 2 cut(s) 276, 615
Bse3DI GCAATG 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 442, 606
BseGI GGATG 2 cut(s) 602, 625
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 3 cut(s) 267, 474, 606
BseXI GCAGC 1 cut(s) 456
BseYI CCCAGC 1 cut(s) 413
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 113, 157
BshNI GGYRCC 1 cut(s) 610
BslI CCNNNNNNNGG 1 cut(s) 414
BsmAI GTCTC 2 cut(s) 343, 513
BsnI GGCC 2 cut(s) 113, 157
Bsp1720I GCTNAGC 1 cut(s) 207
BspACI CCGC 5 cut(s) 22, 137, 154, 185, 472
BspANI GGCC 2 cut(s) 113, 157
BspCNI CTCAG 3 cut(s) 268, 475, 607
BspFNI CGCG 1 cut(s) 187
BspLI GGNNCC 3 cut(s) 98, 600, 612
BspMI ACCTGC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 610
BsrDI GCAATG 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 442, 606
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 313
BstDEI CTNAG 4 cut(s) 207, 276, 483, 615
BstF5I GGATG 2 cut(s) 602, 625
BstFNI CGCG 1 cut(s) 187
BstHHI GCGC 1 cut(s) 189
BstMAI GTCTC 2 cut(s) 343, 513
BstMWI GCNNNNNNNGC 3 cut(s) 151, 203, 466
BstNSI RCATGY 1 cut(s) 238
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 456
BstV2I GAAGAC 1 cut(s) 660
Bsu36I CCTNAGG 2 cut(s) 276, 615
BsuRI GGCC 2 cut(s) 113, 157
BtgZI GCGATG 2 cut(s) 342, 399
BtsCI GGATG 2 cut(s) 602, 625
BveI ACCTGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 3 cut(s) 13, 96, 156
Csp6I GTAC 1 cut(s) 262
CviAII CATG 3 cut(s) 134, 175, 235
CviJI RGCY 8 cut(s) 84, 113, 157, 206, 226, 418, 431, 469
CviKI_1 RGCY 8 cut(s) 84, 113, 157, 206, 226, 418, 431, 469
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 4 cut(s) 207, 276, 483, 615
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 13, 96
Eco81I CCTNAGG 2 cut(s) 276, 615
FaeI CATG 3 cut(s) 137, 178, 238
FatI CATG 3 cut(s) 133, 174, 234
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 155, 470
FokI GGATG 2 cut(s) 589, 632
Fsp4HI GCNGC 2 cut(s) 155, 470
FspBI CTAG 1 cut(s) 512
GlaI GCGC 1 cut(s) 188
GluI GCNGC 2 cut(s) 155, 470
GsaI CCCAGC 1 cut(s) 417
HaeIII GGCC 2 cut(s) 113, 157
HhaI GCGC 1 cut(s) 189
Hin1II CATG 3 cut(s) 137, 178, 238
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 2 cut(s) 506, 569
HpaI GTTAAC 1 cut(s) 477
HphI GGTGA 3 cut(s) 25, 541, 650
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 5 cut(s) 96, 232, 443, 574, 607
Hpy188III TCNNGA 4 cut(s) 275, 482, 512, 581
Hpy8I GTNNAC 1 cut(s) 477
HpyAV CCTTC 3 cut(s) 49, 391, 450
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4V TGCA 3 cut(s) 174, 259, 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 203, 466
HpyF3I CTNAG 4 cut(s) 207, 276, 483, 615
Hsp92II CATG 3 cut(s) 137, 178, 238
HspAI GCGC 1 cut(s) 187
KspAI GTTAAC 1 cut(s) 477
LmnI GCTCC 2 cut(s) 150, 223
LpnPI CCDG 9 cut(s) 113, 154, 288, 338, 399, 495, 511, 627, 660
Lsp1109I GCAGC 1 cut(s) 456
MaeI CTAG 1 cut(s) 512
MaeIII GTNAC 1 cut(s) 299
MboII GAAGA 4 cut(s) 263, 300, 512, 665
MfeI CAATTG 1 cut(s) 593
MluCI AATT 4 cut(s) 90, 449, 576, 593
MlyI GAGTC 1 cut(s) 515
MseI TTAA 3 cut(s) 242, 360, 476
MunI CAATTG 1 cut(s) 593
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 3 cut(s) 151, 203, 466
NlaIII CATG 3 cut(s) 137, 178, 238
NlaIV GGNNCC 3 cut(s) 98, 600, 612
NspI RCATGY 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 569
PkrI GCNGC 2 cut(s) 156, 471
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
PspFI CCCAGC 1 cut(s) 413
PspN4I GGNNCC 3 cut(s) 98, 600, 612
PspPI GGNCC 3 cut(s) 13, 96, 156
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SaqAI TTAA 3 cut(s) 242, 360, 476
SatI GCNGC 2 cut(s) 155, 470
Sau96I GGNCC 3 cut(s) 13, 96, 156
SchI GAGTC 1 cut(s) 515
SinI GGWCC 2 cut(s) 13, 96
Sse9I AATT 4 cut(s) 90, 449, 576, 593
SsiI CCGC 5 cut(s) 22, 137, 154, 185, 472
SspMI CTAG 1 cut(s) 512
TaaI ACNGT 1 cut(s) 299
TasI AATT 4 cut(s) 90, 449, 576, 593
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 3 cut(s) 242, 360, 476
Tru9I TTAA 3 cut(s) 242, 360, 476
TseI GCWGC 1 cut(s) 469
TspDTI ATGAA 5 cut(s) 402, 479, 484, 573, 666
TspGWI ACGGA 2 cut(s) 137, 639
VpaK11BI GGWCC 2 cut(s) 13, 96
XapI RAATTY 1 cut(s) 576
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.