Prupe.8G082300_v2.0.a1

ribosome-binding factor A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0012528
Physical Location & Seq
Forward (+)
11535775 .. 11539343
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G082300.1

Sequence Viewer

Length: 672 bp
ATGCCACAAGTGGTCCTCCATCACCCACTCCTCACGCCCTGCCTTTCTCACCTTCCATCATCATCATCAACATCAAGCTTTCCAATTCGAACCCAGAAATCAACGGCCTGGATTCCCCAAAGAAATCATGCGGGTCTGCTCCGCTGGCATCCCACCACCACCATAAGGTGCATGGCTAACCCTCGCAGAGTTGCAATGGTGGCTAAGCAGATAAGGAGGGAGCTTTCTGATATGCTCTTAACTGATAATGTCTTGCAGTACGCTATTCTTCCTGAGGTCGCTTTGGGTGCTGACCGTTATCTCTCTTCTTTGACCACCATTAGTGATGTTGAAGTTTCTACTGACTTGCAGGTGGTTAAAGTATATGTTTCTGTTTTTGGCGATGAAAGAGGAAAGGAGGTTGCCCTTGCGGGGCTAAAGTCAAAAGCCAAATATGTCCGCGGTGAATTGGGAAAGCGTATGAAGCTGCGGTTGACTCCTGAGATACGCTTCATAGAAGACGAGTCTTTAGAGAGAGGAAGCAGGGTTATTGCAATATTAGATAAGATAAAGAATGAAAAGAAAGATCCCGACATTCAAGATGAGGAGCAATTGGAATCATCCGAGGCACCTCAGGATGACACAGATTGGGAGGGTGATGACCCTGATGAAGACATTATATACATAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009536 GO:0022613 GO:0042254 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RBFA

Protein Analysis

224

Amino Acids

25.21

Weight (kDa)

5.46

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34730 AT4G34730 AT4G34730
fragaria_vesca FvH4_2g10740 FvH4_2g10740
malus_domestica MD00G1066800.v1.1
prunus_persica Prupe.8G082300_v2.0.a1
pyrus_communis pycom05g04460 pycom10g04740
rosa_chinensis RchiOBHm_Chr6g0268531
rosa_laevigata RLG00000013905
rosa_multiflora Rmu_sc0012389.1_g000002
rosa_roxburghii Rroxscaffold_7G00199750
rosa_rugosa Rorug06G0042900
rosa_samantha Rh6AG162300 Rh6BG166500 Rh6CG160300 Rh6DG151700
rosa_wichuraiana Rw6G014010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 607
AccII CGCG 1 cut(s) 441
AciI CCGC 6 cut(s) 131, 142, 410, 439, 441, 469
AclWI GGATC 1 cut(s) 560
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 2 cut(s) 165, 411
AgsI TTSAA 2 cut(s) 332, 578
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 3 cut(s) 78, 223, 466
AluI AGCT 3 cut(s) 78, 223, 466
AlwI GGATC 1 cut(s) 560
AoxI GGCC 1 cut(s) 105
ApeKI GCWGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 13
AsuHPI GGTGA 4 cut(s) 14, 41, 455, 647
AsuII TTCGAA 1 cut(s) 88
AvaII GGWCC 1 cut(s) 13
AxyI CCTNAGG 2 cut(s) 273, 612
BanI GGYRCC 1 cut(s) 607
BbsI GAAGAC 2 cut(s) 504, 657
BbvI GCAGC 1 cut(s) 453
BccI CCATC 2 cut(s) 27, 64
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 1 cut(s) 109
BfaI CTAG 1 cut(s) 670
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 1 cut(s) 467
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 468
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 609
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 2 cut(s) 504, 657
Bpu1102I GCTNAGC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 88
BsaJI CCNNGG 2 cut(s) 439, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 411
Bse21I CCTNAGG 2 cut(s) 273, 612
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 439, 603
BseGI GGATG 3 cut(s) 148, 599, 622
BseLI CCNNNNNNNGG 2 cut(s) 165, 411
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 3 cut(s) 264, 471, 626
BseRI GAGGAG 2 cut(s) 20, 599
BseXI GCAGC 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 441
BshFI GGCC 1 cut(s) 107
BshNI GGYRCC 1 cut(s) 607
BslI CCNNNNNNNGG 2 cut(s) 165, 411
BsnI GGCC 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 88
Bsp143I GATC 1 cut(s) 565
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 6 cut(s) 131, 142, 410, 439, 441, 469
BspANI GGCC 1 cut(s) 107
BspCNI CTCAG 3 cut(s) 265, 472, 625
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 609
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 1 cut(s) 560
BspT104I TTCGAA 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 607
BsrDI GCAATG 1 cut(s) 201
BssECI CCNNGG 2 cut(s) 439, 603
BssMI GATC 1 cut(s) 565
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 1 cut(s) 310
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 4 cut(s) 204, 273, 480, 612
BstDSI CCRYGG 1 cut(s) 439
BstF5I GGATG 3 cut(s) 148, 599, 622
BstFNI CGCG 1 cut(s) 441
BstKTI GATC 1 cut(s) 568
BstMBI GATC 1 cut(s) 565
BstMWI GCNNNNNNNGC 4 cut(s) 145, 200, 287, 463
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 107
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 2 cut(s) 504, 657
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
Bsu36I CCTNAGG 2 cut(s) 273, 612
BsuRI GGCC 1 cut(s) 107
BtgI CCRYGG 1 cut(s) 439
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 3 cut(s) 148, 599, 622
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 13
Cfr42I CCGCGG 1 cut(s) 442
Csp6I GTAC 1 cut(s) 259
CviAII CATG 2 cut(s) 128, 172
CviJI RGCY 8 cut(s) 78, 107, 176, 203, 223, 415, 428, 466
CviKI_1 RGCY 8 cut(s) 78, 107, 176, 203, 223, 415, 428, 466
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 4 cut(s) 204, 273, 480, 612
DpnI GATC 1 cut(s) 567
DpnII GATC 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 13
Eco81I CCTNAGG 2 cut(s) 273, 612
EcoRII CCWGG 1 cut(s) 107
FaeI CATG 2 cut(s) 131, 175
FatI CATG 2 cut(s) 127, 171
FauI CCCGC 2 cut(s) 124, 403
Fnu4HI GCNGC 1 cut(s) 467
FokI GGATG 3 cut(s) 135, 586, 629
Fsp4HI GCNGC 1 cut(s) 467
FspBI CTAG 1 cut(s) 670
GluI GCNGC 1 cut(s) 467
HaeIII GGCC 1 cut(s) 107
Hin1II CATG 2 cut(s) 131, 175
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 4 cut(s) 112, 475, 503, 596
HphI GGTGA 4 cut(s) 14, 41, 455, 647
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 2 cut(s) 229, 604
Hpy188III TCNNGA 5 cut(s) 272, 479, 569, 578, 614
Hpy8I GTNNAC 1 cut(s) 474
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 5 cut(s) 171, 194, 256, 349, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 200, 287, 463
HpyF3I CTNAG 4 cut(s) 204, 273, 480, 612
Hsp92II CATG 2 cut(s) 131, 175
KspI CCGCGG 1 cut(s) 442
Kzo9I GATC 1 cut(s) 565
LmnI GCTCC 3 cut(s) 144, 220, 586
Lsp1109I GCAGC 1 cut(s) 453
LweI GCATC 1 cut(s) 157
MaeI CTAG 1 cut(s) 670
MalI GATC 1 cut(s) 567
MboI GATC 1 cut(s) 565
MboII GAAGA 4 cut(s) 260, 297, 509, 662
MfeI CAATTG 1 cut(s) 590
MflI RGATCY 1 cut(s) 565
MluCI AATT 3 cut(s) 84, 446, 590
MlyI GAGTC 2 cut(s) 469, 512
MseI TTAA 2 cut(s) 239, 357
MspA1I CMGCKG 2 cut(s) 144, 441
MspR9I CCNGG 1 cut(s) 109
MunI CAATTG 1 cut(s) 590
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 4 cut(s) 145, 200, 287, 463
NdeII GATC 1 cut(s) 565
NlaIII CATG 2 cut(s) 131, 175
NlaIV GGNNCC 1 cut(s) 609
NspV TTCGAA 1 cut(s) 88
PaqCI CACCTGC 1 cut(s) 340
PfeI GAWTC 2 cut(s) 112, 596
PkrI GCNGC 1 cut(s) 468
PleI GAGTC 2 cut(s) 469, 511
PpsI GAGTC 2 cut(s) 469, 511
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 609
PspPI GGNCC 1 cut(s) 13
PsuI RGATCY 1 cut(s) 565
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SacII CCGCGG 1 cut(s) 442
SaqAI TTAA 2 cut(s) 239, 357
SatI GCNGC 1 cut(s) 467
Sau3AI GATC 1 cut(s) 565
Sau96I GGNCC 1 cut(s) 13
SchI GAGTC 2 cut(s) 469, 512
ScrFI CCNGG 1 cut(s) 109
SetI ASST 9 cut(s) 54, 80, 170, 225, 279, 354, 402, 468, 613
SfaNI GCATC 1 cut(s) 157
Sfr303I CCGCGG 1 cut(s) 442
SfuI TTCGAA 1 cut(s) 88
SgrBI CCGCGG 1 cut(s) 442
SinI GGWCC 1 cut(s) 13
Sse9I AATT 3 cut(s) 84, 446, 590
SsiI CCGC 6 cut(s) 131, 142, 410, 439, 441, 469
SspI AATATT 1 cut(s) 537
SspMI CTAG 1 cut(s) 670
StyD4I CCNGG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 88
TasI AATT 3 cut(s) 84, 446, 590
TfiI GAWTC 2 cut(s) 112, 596
Tru1I TTAA 2 cut(s) 239, 357
Tru9I TTAA 2 cut(s) 239, 357
TseI GCWGC 1 cut(s) 466
TspDTI ATGAA 5 cut(s) 399, 476, 481, 570, 663
VpaK11BI GGWCC 1 cut(s) 13
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XspI CTAG 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.