Rw6G014010

ribosome-binding factor A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012528
Physical Location & Seq
Reverse (-)
25875598 .. 25878777
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014010.1

Sequence Viewer

Length: 648 bp
ATGCCATACTTGGTTCTCCACAGCCCACCACTCGCGTCCTTCCCTTGTCACCTTCCATCATCAAGGTTCCCAATTCAGACCCAGAAGCCAGCGGCCTGGATTCACCAAAGAAATCATCATGCGGCTCTGCTCCGCGGCATAAGGTGCATGGCGAAGCCGCGCAGAGTGGCAATGGTGGCTAAGCAGATACAGAGGGAGCTCTCTGACATGCTCTTAACAGATAAGGTGTTGCAATACGCTATTTTGCCTGAGGTTTCTTTGGGTGCCGACCGCTACCTCTCTTCTTTAACCACAATTAGTGATGTTGAGGTCTCTACCGACTTGCAGGTGGTTAAAGTATTTGTATCCGTTTTTGGCGATGAAAGAGGGAAGGAGGTTGCACTTGCAGGGCTAAAGTCAAAAGCAAAGTACGTCCGTGGTGAATTGGGAAGGCGTATGAAACTGCGATTAACTCCTGAGATACGCTTCATAGAAGATGAATCGTTAGAGAGAGGAAGCAGGGTGATTGCAATATTAGATCGGATAAAAAATGAGAAGAAAGTTGGAGAAATTCAAGATGAGGAGGAATTGGAATCATCCAACGCACCTCAGGATGATAAAGACTGGGAGGATGATGACCCTGATGAAGACATTATATACATAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009536 GO:0022613 GO:0042254 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RBFA

Protein Analysis

215

Amino Acids

24.47

Weight (kDa)

6.01

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBFA PF02033 55 - 171 2.3e-26 Ribosome-binding factor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34730 AT4G34730 AT4G34730
fragaria_vesca FvH4_2g10740 FvH4_2g10740
malus_domestica MD00G1066800.v1.1
prunus_persica Prupe.8G082300_v2.0.a1
pyrus_communis pycom05g04460 pycom10g04740
rosa_chinensis RchiOBHm_Chr6g0268531
rosa_laevigata RLG00000013905
rosa_multiflora Rmu_sc0012389.1_g000002
rosa_roxburghii Rroxscaffold_7G00199750
rosa_rugosa Rorug06G0042900
rosa_samantha Rh6AG162300 Rh6BG166500 Rh6CG160300 Rh6DG151700
rosa_wichuraiana Rw6G014010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 316
Acc36I ACCTGC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 263
AccII CGCG 3 cut(s) 35, 135, 160
AciI CCGC 6 cut(s) 92, 122, 133, 135, 158, 271
AcsI RAATTY 1 cut(s) 549
AfaI GTAC 1 cut(s) 410
AgsI TTSAA 1 cut(s) 554
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 1 cut(s) 199
AluI AGCT 1 cut(s) 199
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 316
AoxI GGCC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 549
AspLEI GCGC 1 cut(s) 162
AsuHPI GGTGA 4 cut(s) 41, 95, 431, 514
AxyI CCTNAGG 2 cut(s) 249, 588
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 201
BccI CCATC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 97
BciVI GTATCC 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 316
BfuI GTATCC 1 cut(s) 355
BisI GCNGC 4 cut(s) 93, 123, 136, 158
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 4 cut(s) 94, 124, 137, 159
Bme1390I CCNGG 1 cut(s) 97
BmiI GGNNCC 2 cut(s) 68, 265
BmrFI CCNGG 1 cut(s) 97
BmrI ACTGGG 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 2 cut(s) 133, 415
Bse1I ACTGG 1 cut(s) 608
Bse21I CCTNAGG 2 cut(s) 249, 588
Bse3DI GCAATG 1 cut(s) 177
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 2 cut(s) 133, 415
BseGI GGATG 3 cut(s) 575, 598, 616
BseMI GCAATG 1 cut(s) 177
BseMII CTCAG 3 cut(s) 240, 447, 602
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 1 cut(s) 575
Bsh1236I CGCG 3 cut(s) 35, 135, 160
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 263
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 201
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 1 cut(s) 95
Bso31I GGTCTC 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 1 cut(s) 517
Bsp1720I GCTNAGC 1 cut(s) 180
BspACI CCGC 6 cut(s) 92, 122, 133, 135, 158, 271
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 3 cut(s) 241, 448, 601
BspFNI CGCG 3 cut(s) 35, 135, 160
BspLI GGNNCC 2 cut(s) 68, 265
BspMI ACCTGC 1 cut(s) 316
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 177
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 2 cut(s) 133, 415
BssMI GATC 1 cut(s) 517
Bst2UI CCWGG 1 cut(s) 97
Bst6I CTCTTC 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 4 cut(s) 180, 249, 456, 588
BstDSI CCRYGG 2 cut(s) 133, 415
BstF5I GGATG 3 cut(s) 575, 598, 616
BstFNI CGCG 3 cut(s) 35, 135, 160
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 1 cut(s) 517
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 2 cut(s) 144, 176
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 95
BstUI CGCG 3 cut(s) 35, 135, 160
BstV2I GAAGAC 1 cut(s) 633
BstXI CCANNNNNNTGG 1 cut(s) 96
Bsu36I CCTNAGG 2 cut(s) 249, 588
BsuI GTATCC 1 cut(s) 355
BsuRI GGCC 1 cut(s) 95
BtgI CCRYGG 2 cut(s) 133, 415
BtgZI GCGATG 1 cut(s) 372
BtsCI GGATG 3 cut(s) 575, 598, 616
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 90
CfoI GCGC 1 cut(s) 162
Cfr42I CCGCGG 1 cut(s) 136
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 409
CviAII CATG 3 cut(s) 119, 148, 208
CviJI RGCY 8 cut(s) 24, 88, 95, 125, 157, 179, 199, 391
CviKI_1 RGCY 8 cut(s) 24, 88, 95, 125, 157, 179, 199, 391
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 4 cut(s) 180, 249, 456, 588
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 199
Eco81I CCTNAGG 2 cut(s) 249, 588
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 201
FaeI CATG 3 cut(s) 122, 151, 211
FatI CATG 3 cut(s) 118, 147, 207
Fnu4HI GCNGC 4 cut(s) 93, 123, 136, 158
FokI GGATG 3 cut(s) 562, 605, 623
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 4 cut(s) 93, 123, 136, 158
GlaI GCGC 1 cut(s) 161
GluI GCNGC 4 cut(s) 93, 123, 136, 158
HaeIII GGCC 1 cut(s) 95
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 162
Hin1II CATG 3 cut(s) 122, 151, 211
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 3 cut(s) 100, 479, 572
HphI GGTGA 4 cut(s) 41, 95, 431, 514
Hpy188I TCNGA 3 cut(s) 78, 205, 522
Hpy188III TCNNGA 3 cut(s) 455, 554, 590
HpyAV CCTTC 4 cut(s) 49, 62, 364, 423
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 6 cut(s) 147, 232, 325, 380, 386, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 176
HpyF3I CTNAG 4 cut(s) 180, 249, 456, 588
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 3 cut(s) 122, 151, 211
HspAI GCGC 1 cut(s) 160
KspI CCGCGG 1 cut(s) 136
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 2 cut(s) 135, 196
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 1 cut(s) 47
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 4 cut(s) 273, 485, 547, 638
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 5 cut(s) 72, 294, 422, 549, 566
MmeI TCCRAC 2 cut(s) 523, 603
MseI TTAA 4 cut(s) 215, 287, 333, 449
MspA1I CMGCKG 2 cut(s) 92, 135
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 3 cut(s) 35, 135, 160
MwoI GCNNNNNNNGC 2 cut(s) 144, 176
NdeII GATC 1 cut(s) 517
NlaIII CATG 3 cut(s) 122, 151, 211
NlaIV GGNNCC 2 cut(s) 68, 265
NmuCI GTSAC 1 cut(s) 47
NspI RCATGY 1 cut(s) 211
PaqCI CACCTGC 1 cut(s) 316
PfeI GAWTC 3 cut(s) 100, 479, 572
PkrI GCNGC 4 cut(s) 94, 124, 137, 159
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 68, 265
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
SacI GAGCTC 1 cut(s) 201
SacII CCGCGG 1 cut(s) 136
SaqAI TTAA 4 cut(s) 215, 287, 333, 449
SatI GCNGC 4 cut(s) 93, 123, 136, 158
Sau3AI GATC 1 cut(s) 517
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 1 cut(s) 201
Sfr303I CCGCGG 1 cut(s) 136
SgrBI CCGCGG 1 cut(s) 136
Sse9I AATT 5 cut(s) 72, 294, 422, 549, 566
SsiI CCGC 6 cut(s) 92, 122, 133, 135, 158, 271
SspI AATATT 1 cut(s) 513
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 95
TaiI ACGT 1 cut(s) 414
TasI AATT 5 cut(s) 72, 294, 422, 549, 566
TauI GCSGC 4 cut(s) 95, 125, 138, 160
TfiI GAWTC 3 cut(s) 100, 479, 572
Tru1I TTAA 4 cut(s) 215, 287, 333, 449
Tru9I TTAA 4 cut(s) 215, 287, 333, 449
TseFI GTSAC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 5 cut(s) 375, 452, 457, 492, 639
TspGWI ACGGA 2 cut(s) 337, 404
XapI RAATTY 1 cut(s) 549
XceI RCATGY 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.