MD00G1089500.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013040
Physical Location & Seq
Forward (+)
18488276 .. 18490083
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1089500.v1.1.491

Sequence Viewer

Length: 324 bp
ATGAAAATTGGAGAAAGCAGAAGAGCATGTGGCATTTGGCTCTTACCGGTTAAAATTCCCCCGTTTCTCTCTTTCCCTCCGCCGCTCTCTCCTCCATCTCCGTCCCCAAACGCTAGGTGTGTCTCCGCCAAACCACTAGATATGGGAGGCAGAGGAGTTATCGGGGATCGATGGTCCATGAGGATTCTCTGGGCGTGTGCAATTGGAAGTGCTGTCAGCCTATATATGGTTGCTGTGGACAGACAACTAAAGAACAGGGAACGAGCGCTGGCCGAAGAGTTAAAAGCCATGGAGGCAGAATCAGGCAGCGGTGAGGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

108

Amino Acids

11.55

Weight (kDa)

9.3

Isoelectric Point (pI)

66.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 85
AciI CCGC 4 cut(s) 80, 83, 126, 309
AclWI GGATC 1 cut(s) 174
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 1 cut(s) 54
AfeI AGCGCT 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 226
AgeI ACCGGT 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 174
Aor51HI AGCGCT 1 cut(s) 267
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 306
ApoI RAATTY 1 cut(s) 54
AsiGI ACCGGT 1 cut(s) 46
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 323
AvaII GGWCC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 318
BccI CCATC 2 cut(s) 103, 165
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 2 cut(s) 114, 137
BfoI RGCGCY 1 cut(s) 269
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 2 cut(s) 83, 307
BlsI GCNGC 2 cut(s) 84, 308
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 174
Bsa29I ATCGAT 1 cut(s) 169
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse118I RCCGGY 1 cut(s) 46
BseCI ATCGAT 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 288
BseLI CCNNNNNNNGG 1 cut(s) 226
BseRI GAGGAG 2 cut(s) 81, 168
BseXI GCAGC 1 cut(s) 318
BshFI GGCC 1 cut(s) 272
BshTI ACCGGT 1 cut(s) 46
BshVI ATCGAT 1 cut(s) 169
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 272
Bsp143I GATC 1 cut(s) 166
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 4 cut(s) 80, 83, 126, 309
BspANI GGCC 1 cut(s) 272
BspDI ATCGAT 1 cut(s) 169
BspPI GGATC 1 cut(s) 174
BspQI GCTCTTC 1 cut(s) 16
BsrBI CCGCTC 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 46
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 1 cut(s) 166
BssT1I CCWWGG 1 cut(s) 288
Bst6I CTCTTC 2 cut(s) 16, 270
BstC8I GCNNGC 1 cut(s) 270
BstDSI CCRYGG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 318
Bsu15I ATCGAT 1 cut(s) 169
BsuRI GGCC 1 cut(s) 272
BsuTUI ATCGAT 1 cut(s) 169
BtgI CCRYGG 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 270
CfoI GCGC 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 169
CspAI ACCGGT 1 cut(s) 46
CviAII CATG 3 cut(s) 27, 178, 289
CviJI RGCY 4 cut(s) 40, 219, 272, 287
CviKI_1 RGCY 4 cut(s) 40, 219, 272, 287
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 2 cut(s) 16, 270
EarI CTCTTC 2 cut(s) 16, 270
EciI GGCGGA 2 cut(s) 69, 115
Eco130I CCWWGG 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 174
Eco47III AGCGCT 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 3 cut(s) 30, 181, 292
FaiI YATR 7 cut(s) 28, 143, 179, 223, 225, 227, 290
FaqI GGGAC 1 cut(s) 88
FatI CATG 3 cut(s) 26, 177, 288
Fnu4HI GCNGC 2 cut(s) 83, 307
Fsp4HI GCNGC 2 cut(s) 83, 307
FspBI CTAG 2 cut(s) 114, 137
GlaI GCGC 1 cut(s) 267
GluI GCNGC 2 cut(s) 83, 307
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 1 cut(s) 272
HapII CCGG 1 cut(s) 47
HhaI GCGC 1 cut(s) 268
Hin1II CATG 3 cut(s) 30, 181, 292
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 2 cut(s) 184, 299
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 238
Hpy8I GTNNAC 1 cut(s) 238
HpyCH4V TGCA 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
Hsp92II CATG 3 cut(s) 30, 181, 292
HspAI GCGC 1 cut(s) 266
Kzo9I GATC 1 cut(s) 166
LguI GCTCTTC 1 cut(s) 16
LpnPI CCDG 5 cut(s) 60, 175, 241, 254, 288
Lsp1109I GCAGC 1 cut(s) 318
MaeI CTAG 2 cut(s) 114, 137
MalI GATC 1 cut(s) 168
MbiI CCGCTC 1 cut(s) 85
MboI GATC 1 cut(s) 166
MboII GAAGA 2 cut(s) 33, 287
MfeI CAATTG 1 cut(s) 201
MluCI AATT 3 cut(s) 6, 54, 201
MnlI CCTC 7 cut(s) 87, 102, 140, 146, 174, 286, 307
MseI TTAA 2 cut(s) 51, 281
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 1 cut(s) 47
MunI CAATTG 1 cut(s) 201
MwoI GCNNNNNNNGC 1 cut(s) 293
NcoI CCATGG 1 cut(s) 288
NdeII GATC 1 cut(s) 166
NlaIII CATG 3 cut(s) 30, 181, 292
NspI RCATGY 1 cut(s) 30
PciSI GCTCTTC 1 cut(s) 16
PfeI GAWTC 2 cut(s) 184, 299
PinAI ACCGGT 1 cut(s) 46
PkrI GCNGC 2 cut(s) 84, 308
PspPI GGNCC 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 51, 281
SatI GCNGC 2 cut(s) 83, 307
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 1 cut(s) 174
SetI ASST 2 cut(s) 119, 318
SinI GGWCC 1 cut(s) 174
Sse9I AATT 3 cut(s) 6, 54, 201
SsiI CCGC 4 cut(s) 80, 83, 126, 309
SspMI CTAG 2 cut(s) 114, 137
StyI CCWWGG 1 cut(s) 288
TaqI TCGA 1 cut(s) 169
TasI AATT 3 cut(s) 6, 54, 201
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 2 cut(s) 184, 299
Tru1I TTAA 2 cut(s) 51, 281
Tru9I TTAA 2 cut(s) 51, 281
TseI GCWGC 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 90
VpaK11BI GGWCC 1 cut(s) 174
XapI RAATTY 1 cut(s) 54
XceI RCATGY 1 cut(s) 30
XspI CTAG 2 cut(s) 114, 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.