Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004966

Physical Location & Seq

Genomic Coordinates Forward (+)

19896942 .. 19900735

Gene Structure 3794 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1095000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

ATGGAGGATTCTGCCGCTGCTGACATTAATCGCTTCTTTCGTAGTATTTTTCCATTTCCAGTTTCCCTCCCAGTTGAGATAAAGGATGGGAAGATTGCCTCCATTGTTAAAGAAGAGGAAAAGCATGGGAAAATCAAGCTGGAGAATGTGATGGTTTATGGAGAGGCTGTTGTCATGCCTGGCTTGATTGATGTGCACGCACATCTCGATGATCCTGGAAGAGCTGAATGGGAAGGATTTCCATCAGGAATAAAAGCCGCTGCTGCTGGCGGGATAACAACATTGGTTGACATGCCTCTAAATAGTGAGCCATCAACCGTCTCTAGAGAGACCTTGGAACTCAAGATTAAGGCAGCGAAGAGCAGAATTTACGTTGATGTTGGTTTTTGGGGAGGTCTTGTTCCTGAAAATGCTTTCAACGCATCTGCTCTCGAGGATCTCTTAGATGCCGGTGTTCTTGGTCTAAAGTCTTTCATGTGTCCATCCGGGATTAACGACTTTCCTATGACAAATGCCAGTCACATTAAGGAGGGACTATCTGTACTAGCAAAATACAGAAGGCCTTTACTTGTACATGCAGAGAAAGCACAAGAGCTCAAAAGCGACTTGGGAGTTGAAGGTGGTAGTAACGATCCTCGTTCTTATTTGACATATCTCAAGACCAGGCCACCGTCTTGGGAAGAGGCAGCAATTAGGGATCTCTTGACCTTGACAAAGGACACGAGAATTGGTGGCCTGGCAGAAGGAGCTCATCTTCACATCGTTCACTTGTCAGACGCACGCTCTTCGTTAGATCTTATTAAGGAAGCGAAAAGTGGTGGTGATGGTCTAACTATTGAGACGTGCCCCCACTACTTAGCTTTCTCAGCAGAAGAGATTCGCGATGGAGATACACGTTTCAAGTGTTCTCCACCCATCCGCAATGCAGCCAACAAGGAAAAACTGTGGGAGGCTTTGTTGGACGGACATGTTGACATGCTAAGCTCTGATCACTCACCAACTGTGCCAGAACTTAAGCTTCTCAACGACGGAGACTTTTTGAGGGCTTGGGGTGGCATATCATCATTGAAGTTTGTTCTTCCTGTGACATGGTCATATGGCAAGAAATATGGAGTGACATTACAACAGTTAGCTTTATGGTGGAGCGAAAGGCCGGCCAGGCTTGCTGGACAAAAATTGAAGGGGGCAATTGCAGTCGGGAACCATGCTGATATCGCAGTATGGGATCCGGATGTGGAGTTTGACCTCGATGATGAATTTCCCACCCATGTTAAACATCCGGGCATTTCAGCATACTTGGGGACCAAGTTATCTGGAAGAGTTGCAGCCACATTTGTAAGAGGAAACCTTGTCTTCAAAGAGGGGAAACATGCTCCTGCACTCTGCGGCATTCCAATTCTGGCGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000255 GO:0000256 GO:0003674 GO:0003824 GO:0004038 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006807 GO:0006950 GO:0006995 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010135 GO:0010136 GO:0012505 GO:0016787 GO:0016810 GO:0016812 GO:0017144 GO:0019439 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034641 GO:0042594 GO:0042737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0043603 GO:0043605 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0050896 GO:0051716 GO:0055086 GO:0071496 GO:0071704 GO:0072521 GO:0072523 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01120 map00230 map01100 map01120

Pfam Domains

Protein Families

Amidohydro_1

Protein Analysis

470

Amino Acids

51.03

Weight (kDa)

5.79

Isoelectric Point (pI)

32.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALN_composite	PF24890	23 - 56	1.9e-09	Allantoinase, composite domain
Amidohydro_1	PF01979	57 - 451	1.4e-24	Amidohydrolase family
DHOase	PF12890	57 - 258	4.1e-07	Dihydro-orotase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004966)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04955 AT4G04955
fragaria_vesca	FvH4_3g09410 FvH4_3g09410 FvH4_3g09410 FvH4_3g09410
malus_domestica	MD00G1095000.v1.1 MD05G1314100.v1.1
prunus_persica	Prupe.4G045000_v2.0.a1
pyrus_communis	pycom05g29210
rosa_chinensis	RchiOBHm_Chr5g0008181
rosa_laevigata	RLG00000031540 RLG00000031544
rosa_multiflora	Rmu_sc0001352.1_g000006 Rmu_sc0001352.1_g000028 Rmu_sc0001626.1_g000019
rosa_roxburghii	Rroxscaffold_1G00068300
rosa_rugosa	Rorug03G0302600 Rorug04G0433600 Rorug04G0433700 Rorug04G0433800 Rorug04G0433900 Rorug04G0433900
rosa_samantha	Rh4AG007300 Rh4AG211200 Rh5AG062500 Rh5BG058600 Rh5CG069000 Rh5DG057900
rosa_wichuraiana	Rw5G005860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	882
AccIII	TCCGGA	1 cut(s)	1228
AciI	CCGC	5 cut(s)	15, 258, 270, 919, 1386
AclWI	GGATC	6 cut(s)	206, 444, 626, 705, 1220, 1233
AcoI	YGGCCR	1 cut(s)	1155
AcsI	RAATTY	2 cut(s)	366, 1256
AfaI	GTAC	2 cut(s)	543, 573
AflII	CTTAAG	1 cut(s)	1013
AflIII	ACRYGT	2 cut(s)	893, 967
AgsI	TTSAA	6 cut(s)	418, 617, 901, 1069, 1180, 1357
AjiI	CACGTC	1 cut(s)	843
AjnI	CCWGG	5 cut(s)	178, 214, 662, 735, 1157
AloI	GAACNNNNNNTCC	2 cut(s)	384, 416
AluBI	AGCT	8 cut(s)	139, 224, 595, 749, 860, 984, 1018, 1133
AluI	AGCT	8 cut(s)	139, 224, 595, 749, 860, 984, 1018, 1133
Alw21I	GWGCWC	3 cut(s)	198, 597, 751
Alw26I	GTCTC	4 cut(s)	323, 325, 833, 1026
Alw44I	GTGCAC	1 cut(s)	194
AlwI	GGATC	6 cut(s)	206, 444, 626, 705, 1220, 1233
Ama87I	CYCGRG	1 cut(s)	431
Aor13HI	TCCGGA	1 cut(s)	1228
AoxI	GGCC	5 cut(s)	560, 665, 733, 1151, 1155
ApaLI	GTGCAC	1 cut(s)	194
ApeKI	GCWGC	7 cut(s)	17, 260, 263, 353, 686, 926, 1325
ApoI	RAATTY	2 cut(s)	366, 1256
AseI	ATTAAT	1 cut(s)	27
Asp700I	GAANNNNTTC	2 cut(s)	237, 876
AspS9I	GGNCC	1 cut(s)	1302
AsuC2I	CCSGG	2 cut(s)	487, 1281
AsuHPI	GGTGA	2 cut(s)	833, 987
AvaI	CYCGRG	1 cut(s)	431
AvaII	GGWCC	1 cut(s)	1302
BaeGI	GKGCMC	2 cut(s)	198, 848
BamHI	GGATCC	1 cut(s)	1225
BanII	GRGCYC	2 cut(s)	597, 751
BauI	CACGAG	1 cut(s)	721
BbsI	GAAGAC	1 cut(s)	1345
Bbv12I	GWGCWC	3 cut(s)	198, 597, 751
BbvI	GCAGC	7 cut(s)	4, 247, 250, 365, 698, 938, 1337
BccI	CCATC	8 cut(s)	80, 145, 250, 319, 490, 818, 878, 923
BcgI	CGANNNNNNTGC	2 cut(s)	768, 802
BciT130I	CCWGG	5 cut(s)	180, 216, 664, 737, 1159
BclI	TGATCA	1 cut(s)	988
BcnI	CCSGG	2 cut(s)	487, 1281
BcoDI	GTCTC	4 cut(s)	323, 325, 833, 1026
BfaI	CTAG	2 cut(s)	324, 545
BfrI	CTTAAG	1 cut(s)	1013
BglI	GCCNNNNNGGC	1 cut(s)	1159
BglII	AGATCT	1 cut(s)	793
BlpI	GCTNAGC	1 cut(s)	980
Bme1390I	CCNGG	7 cut(s)	180, 216, 487, 664, 737, 1159, 1281
Bme18I	GGWCC	1 cut(s)	1302
BmeT110I	CYCGRG	1 cut(s)	431
BmgBI	CACGTC	1 cut(s)	843
BmgT120I	GGNCC	1 cut(s)	1302
BmiI	GGNNCC	3 cut(s)	1202, 1227, 1303
BmrFI	CCNGG	7 cut(s)	180, 216, 487, 664, 737, 1159, 1281
BmrI	ACTGGG	1 cut(s)	65
BmsI	GCATC	2 cut(s)	431, 436
BmuI	ACTGGG	1 cut(s)	65
BpiI	GAAGAC	1 cut(s)	1345
BpmI	CTGGAG	1 cut(s)	161
Bpu1102I	GCTNAGC	1 cut(s)	980
BpuEI	CTTGAG	2 cut(s)	326, 641
BpuMI	CCSGG	2 cut(s)	487, 1281
BsaBI	GATNNNNATC	1 cut(s)	241
BsaI	GGTCTC	1 cut(s)	323
BsaJI	CCNNGG	1 cut(s)	333
BsaWI	WCCGGW	1 cut(s)	1228
BsaXI	ACNNNNNCTCC	6 cut(s)	153, 183, 384, 414, 941, 971
Bse118I	RCCGGY	2 cut(s)	449, 1153
Bse1I	ACTGG	3 cut(s)	59, 71, 516
Bse3DI	GCAATG	1 cut(s)	928
Bse8I	GATNNNNATC	1 cut(s)	241
BseAI	TCCGGA	1 cut(s)	1228
BseBI	CCWGG	5 cut(s)	180, 216, 664, 737, 1159
BseDI	CCNNGG	1 cut(s)	333
BseGI	GGATG	5 cut(s)	91, 482, 915, 1237, 1276
BseJI	GATNNNNATC	1 cut(s)	241
BseMI	GCAATG	1 cut(s)	928
BseMII	CTCAG	1 cut(s)	879
BseNI	ACTGG	3 cut(s)	59, 71, 516
BseSI	GKGCMC	2 cut(s)	198, 848
BseXI	GCAGC	7 cut(s)	4, 247, 250, 365, 698, 938, 1337
BsgI	GTGCAG	1 cut(s)	1362
Bsh1236I	CGCG	1 cut(s)	882
BshFI	GGCC	5 cut(s)	562, 667, 735, 1153, 1157
BsiHKAI	GWGCWC	3 cut(s)	198, 597, 751
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	5 cut(s)	450, 486, 1154, 1229, 1280
BslFI	GGGAC	2 cut(s)	546, 1315
BsmAI	GTCTC	4 cut(s)	323, 325, 833, 1026
BsmBI	CGTCTC	2 cut(s)	325, 833
BsmFI	GGGAC	2 cut(s)	546, 1315
BsmI	GAATGC	1 cut(s)	1389
BsnI	GGCC	5 cut(s)	562, 667, 735, 1153, 1157
Bso31I	GGTCTC	1 cut(s)	323
BsoBI	CYCGRG	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	198, 597, 751, 848
Bsp13I	TCCGGA	1 cut(s)	1228
Bsp1407I	TGTACA	1 cut(s)	571
Bsp143I	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
Bsp1720I	GCTNAGC	1 cut(s)	980
Bsp68I	TCGCGA	1 cut(s)	882
BspACI	CCGC	5 cut(s)	15, 258, 270, 919, 1386
BspANI	GGCC	5 cut(s)	562, 667, 735, 1153, 1157
BspCNI	CTCAG	1 cut(s)	878
BspEI	TCCGGA	1 cut(s)	1228
BspFNI	CGCG	1 cut(s)	882
BspLI	GGNNCC	3 cut(s)	1202, 1227, 1303
BspPI	GGATC	6 cut(s)	206, 444, 626, 705, 1220, 1233
BspQI	GCTCTTC	3 cut(s)	214, 353, 790
BspTI	CTTAAG	1 cut(s)	1013
BspTNI	GGTCTC	1 cut(s)	323
BsrDI	GCAATG	1 cut(s)	928
BsrFI	RCCGGY	2 cut(s)	449, 1153
BsrGI	TGTACA	1 cut(s)	571
BsrI	ACTGG	3 cut(s)	59, 71, 516
BssAI	RCCGGY	2 cut(s)	449, 1153
BssECI	CCNNGG	1 cut(s)	333
BssMI	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
BssSI	CACGAG	1 cut(s)	721
BssT1I	CCWWGG	1 cut(s)	333
Bst2BI	CACGAG	1 cut(s)	721
Bst2UI	CCWGG	5 cut(s)	180, 216, 664, 737, 1159
Bst4CI	ACNGT	5 cut(s)	319, 672, 945, 1003, 1128
Bst6I	CTCTTC	7 cut(s)	108, 214, 353, 675, 790, 867, 1312
BstAFI	CTTAAG	1 cut(s)	1013
BstAUI	TGTACA	1 cut(s)	571
BstC8I	GCNNGC	5 cut(s)	198, 268, 781, 1155, 1164
BstDEI	CTNAG	4 cut(s)	442, 856, 865, 980
BstF5I	GGATG	5 cut(s)	91, 482, 915, 1237, 1276
BstFNI	CGCG	1 cut(s)	882
BstKTI	GATC	7 cut(s)	214, 439, 634, 700, 796, 991, 1228
BstMAI	GTCTC	4 cut(s)	323, 325, 833, 1026
BstMBI	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
BstMWI	GCNNNNNNNGC	8 cut(s)	263, 419, 584, 746, 866, 1159, 1163, 1214
BstNI	CCWGG	5 cut(s)	180, 216, 664, 737, 1159
BstNSI	RCATGY	5 cut(s)	295, 578, 971, 979, 1373
BstSCI	CCNGG	7 cut(s)	178, 214, 485, 662, 735, 1157, 1279
BstSLI	GKGCMC	2 cut(s)	198, 848
BstUI	CGCG	1 cut(s)	882
BstV1I	GCAGC	7 cut(s)	4, 247, 250, 365, 698, 938, 1337
BstV2I	GAAGAC	1 cut(s)	1345
BstX2I	RGATCY	4 cut(s)	436, 697, 793, 1225
BstXI	CCANNNNNNTGG	1 cut(s)	675
BstYI	RGATCY	4 cut(s)	436, 697, 793, 1225
BsuRI	GGCC	5 cut(s)	562, 667, 735, 1153, 1157
BtgZI	GCGATG	1 cut(s)	897
BtrI	CACGTC	1 cut(s)	843
BtsCI	GGATG	5 cut(s)	91, 482, 915, 1237, 1276
BtuMI	TCGCGA	1 cut(s)	882
Cac8I	GCNNGC	5 cut(s)	198, 268, 781, 1155, 1164
Cfr10I	RCCGGY	2 cut(s)	449, 1153
Cfr13I	GGNCC	1 cut(s)	1302
CseI	GACGC	1 cut(s)	785
Csp6I	GTAC	2 cut(s)	542, 572
CviQI	GTAC	2 cut(s)	542, 572
DdeI	CTNAG	4 cut(s)	442, 856, 865, 980
DpnI	GATC	7 cut(s)	213, 438, 633, 699, 795, 990, 1227
DpnII	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
EaeI	YGGCCR	1 cut(s)	1155
Eam1104I	CTCTTC	7 cut(s)	108, 214, 353, 675, 790, 867, 1312
EarI	CTCTTC	7 cut(s)	108, 214, 353, 675, 790, 867, 1312
Ecl136II	GAGCTC	2 cut(s)	595, 749
Eco130I	CCWWGG	1 cut(s)	333
Eco147I	AGGCCT	1 cut(s)	562
Eco24I	GRGCYC	2 cut(s)	597, 751
Eco31I	GGTCTC	1 cut(s)	323
Eco32I	GATATC	1 cut(s)	1213
Eco47I	GGWCC	1 cut(s)	1302
Eco53kI	GAGCTC	2 cut(s)	595, 749
Eco88I	CYCGRG	1 cut(s)	431
EcoICRI	GAGCTC	2 cut(s)	595, 749
EcoRII	CCWGG	5 cut(s)	178, 214, 662, 735, 1157
EcoRV	GATATC	1 cut(s)	1213
EcoT14I	CCWWGG	1 cut(s)	333
EcoT38I	GRGCYC	2 cut(s)	597, 751
ErhI	CCWWGG	1 cut(s)	333
Esp3I	CGTCTC	2 cut(s)	325, 833
FaqI	GGGAC	2 cut(s)	546, 1315
FauI	CCCGC	1 cut(s)	263
FauNDI	CATATG	1 cut(s)	1096
FbaI	TGATCA	1 cut(s)	988
FokI	GGATG	5 cut(s)	98, 469, 902, 1244, 1263
FriOI	GRGCYC	2 cut(s)	597, 751
FseI	GGCCGGCC	1 cut(s)	1157
FspBI	CTAG	2 cut(s)	324, 545
GsuI	CTGGAG	1 cut(s)	161
HaeIII	GGCC	5 cut(s)	562, 667, 735, 1153, 1157
HapII	CCGG	5 cut(s)	450, 486, 1154, 1229, 1280
HgaI	GACGC	1 cut(s)	785
HincII	GTYRAC	2 cut(s)	289, 973
HindII	GTYRAC	2 cut(s)	289, 973
HindIII	AAGCTT	1 cut(s)	1016
HinfI	GANTC	2 cut(s)	8, 877
HpaII	CCGG	5 cut(s)	450, 486, 1154, 1229, 1280
HphI	GGTGA	2 cut(s)	833, 987
Hpy166II	GTNNAC	4 cut(s)	196, 289, 766, 973
Hpy188I	TCNGA	2 cut(s)	775, 988
Hpy8I	GTNNAC	4 cut(s)	196, 289, 766, 973
Hpy99I	CGWCG	1 cut(s)	1031
HpyAV	CCTTC	5 cut(s)	227, 552, 611, 737, 1174
HpyCH4III	ACNGT	5 cut(s)	319, 672, 945, 1003, 1128
HpyCH4IV	ACGT	3 cut(s)	372, 842, 895
HpyCH4V	TGCA	6 cut(s)	196, 578, 926, 1193, 1325, 1379
HpyF10VI	GCNNNNNNNGC	8 cut(s)	263, 419, 584, 746, 866, 1159, 1163, 1214
HpyF3I	CTNAG	4 cut(s)	442, 856, 865, 980
HpySE526I	ACGT	3 cut(s)	372, 842, 895
Kpn2I	TCCGGA	1 cut(s)	1228
KroI	GCCGGC	1 cut(s)	1153
KroNI	GCCGGC	1 cut(s)	1155
Ksp22I	TGATCA	1 cut(s)	988
Kzo9I	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
LguI	GCTCTTC	3 cut(s)	214, 353, 790
LmnI	GCTCC	3 cut(s)	746, 1143, 1378
Lsp1109I	GCAGC	7 cut(s)	4, 247, 250, 365, 698, 938, 1337
LweI	GCATC	2 cut(s)	431, 436
MaeI	CTAG	2 cut(s)	324, 545
MaeII	ACGT	3 cut(s)	372, 842, 895
MaeIII	GTNAC	4 cut(s)	518, 626, 1084, 1114
MalI	GATC	7 cut(s)	213, 438, 633, 699, 795, 990, 1227
MboI	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
MfeI	CAATTG	1 cut(s)	1188
MflI	RGATCY	4 cut(s)	436, 697, 793, 1225
MhlI	GDGCHC	4 cut(s)	198, 597, 751, 848
MluCI	AATT	7 cut(s)	366, 690, 726, 1175, 1188, 1256, 1395
MmeI	TCCRAC	1 cut(s)	939
MroI	TCCGGA	1 cut(s)	1228
MroNI	GCCGGC	1 cut(s)	1153
MroXI	GAANNNNTTC	2 cut(s)	237, 876
MseI	TTAA	8 cut(s)	27, 108, 348, 492, 525, 801, 1014, 1272
MslI	CAYNNNNRTG	1 cut(s)	207
MspA1I	CMGCKG	2 cut(s)	17, 260
MspCI	CTTAAG	1 cut(s)	1013
MspI	CCGG	5 cut(s)	450, 486, 1154, 1229, 1280
MspR9I	CCNGG	7 cut(s)	180, 216, 487, 664, 737, 1159, 1281
MunI	CAATTG	1 cut(s)	1188
Mva1269I	GAATGC	1 cut(s)	1389
MvaI	CCWGG	5 cut(s)	180, 216, 664, 737, 1159
MvnI	CGCG	1 cut(s)	882
MwoI	GCNNNNNNNGC	8 cut(s)	263, 419, 584, 746, 866, 1159, 1163, 1214
NaeI	GCCGGC	1 cut(s)	1155
NciI	CCSGG	2 cut(s)	487, 1281
NdeI	CATATG	1 cut(s)	1096
NdeII	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
NgoMIV	GCCGGC	1 cut(s)	1153
NlaIV	GGNNCC	3 cut(s)	1202, 1227, 1303
NmuCI	GTSAC	3 cut(s)	518, 1084, 1114
NruI	TCGCGA	1 cut(s)	882
NspI	RCATGY	5 cut(s)	295, 578, 971, 979, 1373
PaeR7I	CTCGAG	1 cut(s)	431
PceI	AGGCCT	1 cut(s)	562
PciI	ACATGT	1 cut(s)	967
PciSI	GCTCTTC	3 cut(s)	214, 353, 790
PcsI	WCGNNNNNNNCGW	2 cut(s)	37, 204
PctI	GAATGC	1 cut(s)	1389
PdiI	GCCGGC	1 cut(s)	1155
PdmI	GAANNNNTTC	2 cut(s)	237, 876
PfeI	GAWTC	2 cut(s)	8, 877
PflFI	GACNNNGTC	1 cut(s)	1090
PfoI	TCCNGGA	2 cut(s)	214, 485
PscI	ACATGT	1 cut(s)	967
PshBI	ATTAAT	1 cut(s)	27
Psp124BI	GAGCTC	2 cut(s)	597, 751
Psp6I	CCWGG	5 cut(s)	178, 214, 662, 735, 1157
PspGI	CCWGG	5 cut(s)	178, 214, 662, 735, 1157
PspN4I	GGNNCC	3 cut(s)	1202, 1227, 1303
PspPI	GGNCC	1 cut(s)	1302
PsuI	RGATCY	4 cut(s)	436, 697, 793, 1225
PsyI	GACNNNGTC	1 cut(s)	1090
RigI	GGCCGGCC	1 cut(s)	1157
RruI	TCGCGA	1 cut(s)	882
RsaI	GTAC	2 cut(s)	543, 573
RsaNI	GTAC	2 cut(s)	542, 572
RseI	CAYNNNNRTG	1 cut(s)	207
SacI	GAGCTC	2 cut(s)	597, 751
SapI	GCTCTTC	3 cut(s)	214, 353, 790
SaqAI	TTAA	8 cut(s)	27, 108, 348, 492, 525, 801, 1014, 1272
Sau3AI	GATC	7 cut(s)	211, 436, 631, 697, 793, 988, 1225
Sau96I	GGNCC	1 cut(s)	1302
ScrFI	CCNGG	7 cut(s)	180, 216, 487, 664, 737, 1159, 1281
SduI	GDGCHC	4 cut(s)	198, 597, 751, 848
SfaNI	GCATC	2 cut(s)	431, 436
Sfr274I	CTCGAG	1 cut(s)	431
SinI	GGWCC	1 cut(s)	1302
SlaI	CTCGAG	1 cut(s)	431
SmiMI	CAYNNNNRTG	1 cut(s)	207
SmlI	CTYRAG	4 cut(s)	341, 431, 656, 1013
SmoI	CTYRAG	4 cut(s)	341, 431, 656, 1013
Sse9I	AATT	7 cut(s)	366, 690, 726, 1175, 1188, 1256, 1395
SseBI	AGGCCT	1 cut(s)	562
SsiI	CCGC	5 cut(s)	15, 258, 270, 919, 1386
SspMI	CTAG	2 cut(s)	324, 545
SstI	GAGCTC	2 cut(s)	597, 751
StuI	AGGCCT	1 cut(s)	562
StyD4I	CCNGG	7 cut(s)	178, 214, 485, 662, 735, 1157, 1279
StyI	CCWWGG	1 cut(s)	333
TaaI	ACNGT	5 cut(s)	319, 672, 945, 1003, 1128
TaiI	ACGT	3 cut(s)	375, 845, 898
TaqI	TCGA	3 cut(s)	207, 432, 1248
TasI	AATT	7 cut(s)	366, 690, 726, 1175, 1188, 1256, 1395
TatI	WGTACW	2 cut(s)	541, 571
TauI	GCSGC	3 cut(s)	17, 260, 1389
TfiI	GAWTC	2 cut(s)	8, 877
Tru1I	TTAA	8 cut(s)	27, 108, 348, 492, 525, 801, 1014, 1272
Tru9I	TTAA	8 cut(s)	27, 108, 348, 492, 525, 801, 1014, 1272
TseFI	GTSAC	3 cut(s)	518, 1084, 1114
TseI	GCWGC	7 cut(s)	17, 260, 263, 353, 686, 926, 1325
Tsp45I	GTSAC	3 cut(s)	518, 1084, 1114
TspDTI	ATGAA	2 cut(s)	463, 1269
TspGWI	ACGGA	2 cut(s)	978, 1044
Tth111I	GACNNNGTC	1 cut(s)	1090
Vha464I	CTTAAG	1 cut(s)	1013
VneI	GTGCAC	1 cut(s)	194
VpaK11BI	GGWCC	1 cut(s)	1302
VspI	ATTAAT	1 cut(s)	27
XapI	RAATTY	2 cut(s)	366, 1256
XbaI	TCTAGA	1 cut(s)	323
XceI	RCATGY	5 cut(s)	295, 578, 971, 979, 1373
XhoI	CTCGAG	1 cut(s)	431
XmnI	GAANNNNTTC	2 cut(s)	237, 876
XspI	CTAG	2 cut(s)	324, 545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.