MD00G1098100.v1.1

Late embryogenesis abundant (LEA) group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017358
Physical Location & Seq
Reverse (-)
20469137 .. 20469995
859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1098100.v1.1.491

Sequence Viewer

Length: 333 bp
ATGCAGGCAGTGAAGGACAAGATAAATGAAATAGGCGCCATGAAAAAGGTCAAGGAGGAGGCCAGGGCCGAAGAGAGGGCTGAGAAGGAAATAGCAAAGGCGAGAGCAGACGTAGCTCATGAGGTGCGATTGGCTAAAGAAGCTGAAGCAGCAATGTCACTGCATGTGGCCAAGGCTGGGGAGATTGCCAGAGCTGGAGACAAGGCAGCACATGAACATGAAATTAAGGCTAAGCATGCGGCTGATGGTACGAACACTACGCATGGACATGGAGCTTCTCCTTCTGAACTGGCCGGTGCGGGAGGTCATCCAGCTTATAAACGCATGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

111

Amino Acids

11.65

Weight (kDa)

8.05

Isoelectric Point (pI)

27.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 64 7.6e-11 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017358)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08030
malus_domestica MD00G1098100.v1.1 MD02G1236800.v1.1
prunus_persica Prupe.2G109300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0341271
rosa_laevigata RLG00000029078
rosa_roxburghii Rroxscaffold_4G00311910
rosa_rugosa Rorug01G0158200.1
rosa_samantha Rh1AG172600 Rh1BG140700 Rh1CG160700 Rh1DG172900
rosa_wichuraiana Rw1G014400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 35
AciI CCGC 2 cut(s) 239, 299
AcoI YGGCCR 2 cut(s) 168, 291
AcuI CTGAAG 1 cut(s) 165
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 2 cut(s) 75, 177
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 5 cut(s) 116, 143, 194, 275, 314
AluI AGCT 5 cut(s) 116, 143, 194, 275, 314
Alw26I GTCTC 1 cut(s) 192
AoxI GGCC 4 cut(s) 60, 66, 168, 291
ApeKI GCWGC 2 cut(s) 149, 206
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 66
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 161, 218
BccI CCATC 1 cut(s) 239
BciT130I CCWGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 3 cut(s) 150, 207, 240
BlpI GCTNAGC 1 cut(s) 231
BlsI GCNGC 3 cut(s) 151, 208, 241
Bme1390I CCNGG 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 63, 171
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 177
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 63, 171
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 75, 177
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 161, 218
BseYI CCCAGC 1 cut(s) 176
BshFI GGCC 4 cut(s) 62, 68, 170, 293
BshNI GGYRCC 1 cut(s) 35
BsiSI CCGG 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 75, 177
BsmAI GTCTC 1 cut(s) 192
BsnI GGCC 4 cut(s) 62, 68, 170, 293
Bsp1720I GCTNAGC 1 cut(s) 231
BspACI CCGC 2 cut(s) 239, 299
BspANI GGCC 4 cut(s) 62, 68, 170, 293
BspCNI CTCAG 1 cut(s) 73
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 1 cut(s) 294
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 2 cut(s) 63, 171
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 3 cut(s) 6, 237, 326
BstDEI CTNAG 2 cut(s) 81, 231
BstF5I GGATG 1 cut(s) 307
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 1 cut(s) 38
BstMAI GTCTC 1 cut(s) 192
BstMWI GCNNNNNNNGC 4 cut(s) 113, 140, 149, 236
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 3 cut(s) 167, 239, 328
BstSCI CCNGG 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 161, 218
BsuRI GGCC 4 cut(s) 62, 68, 170, 293
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 2 cut(s) 15, 158
BtsIMutI CAGTG 2 cut(s) 15, 158
Cac8I GCNNGC 3 cut(s) 6, 237, 326
CciI TCATGA 1 cut(s) 118
CfoI GCGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 249
CviAII CATG 9 cut(s) 40, 119, 164, 212, 218, 236, 263, 269, 325
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 2 cut(s) 81, 231
DinI GGCGCC 1 cut(s) 37
EaeI YGGCCR 2 cut(s) 168, 291
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 9 cut(s) 43, 122, 167, 215, 221, 239, 266, 272, 328
FatI CATG 9 cut(s) 39, 118, 163, 211, 217, 235, 262, 268, 324
FauI CCCGC 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 150, 207, 240
FokI GGATG 1 cut(s) 294
Fsp4HI GCNGC 3 cut(s) 150, 207, 240
GlaI GCGC 1 cut(s) 37
GluI GCNGC 3 cut(s) 150, 207, 240
GsaI CCCAGC 1 cut(s) 180
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 4 cut(s) 62, 68, 170, 293
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 9 cut(s) 43, 122, 167, 215, 221, 239, 266, 272, 328
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HpaII CCGG 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 286
Hpy188III TCNNGA 1 cut(s) 119
HpyAV CCTTC 3 cut(s) 7, 79, 291
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 2 cut(s) 4, 163
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 140, 149, 236
HpyF3I CTNAG 2 cut(s) 81, 231
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 9 cut(s) 43, 122, 167, 215, 221, 239, 266, 272, 328
HspAI GCGC 1 cut(s) 36
KasI GGCGCC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 8 cut(s) 49, 76, 162, 180, 202, 275, 307, 324
Lsp1109I GCAGC 2 cut(s) 161, 218
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 156
MboII GAAGA 1 cut(s) 83
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 1 cut(s) 222
MluNI TGGCCA 1 cut(s) 170
Mly113I GGCGCC 1 cut(s) 36
MnlI CCTC 5 cut(s) 49, 52, 69, 115, 296
Mox20I TGGCCA 1 cut(s) 170
MscI TGGCCA 1 cut(s) 170
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 2 cut(s) 216, 267
Msp20I TGGCCA 1 cut(s) 170
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 4 cut(s) 113, 140, 149, 236
NarI GGCGCC 1 cut(s) 36
NlaIII CATG 9 cut(s) 43, 122, 167, 215, 221, 239, 266, 272, 328
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 156
NspI RCATGY 3 cut(s) 167, 239, 328
PaeI GCATGC 2 cut(s) 239, 328
PagI TCATGA 1 cut(s) 118
PkrI GCNGC 3 cut(s) 151, 208, 241
PluTI GGCGCC 1 cut(s) 39
PsiI TTATAA 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 62
PspFI CCCAGC 1 cut(s) 176
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 1 cut(s) 66
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
RseI CAYNNNNRTG 2 cut(s) 216, 267
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 3 cut(s) 150, 207, 240
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 64
SetI ASST 9 cut(s) 51, 114, 118, 126, 145, 196, 277, 307, 316
SfoI GGCGCC 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 216, 267
SphI GCATGC 2 cut(s) 239, 328
Sse9I AATT 1 cut(s) 222
SsiI CCGC 2 cut(s) 239, 299
SspDI GGCGCC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 171
TaiI ACGT 1 cut(s) 114
TasI AATT 1 cut(s) 222
TauI GCSGC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 2 cut(s) 15, 165
TseFI GTSAC 1 cut(s) 156
TseI GCWGC 2 cut(s) 149, 206
Tsp45I GTSAC 1 cut(s) 156
TspDTI ATGAA 4 cut(s) 42, 56, 228, 234
TspRI CASTG 2 cut(s) 15, 165
XceI RCATGY 3 cut(s) 167, 239, 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.