MD02G1236800.v1.1

Late embryogenesis abundant (LEA) group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017358
Physical Location & Seq
Reverse (-)
28355230 .. 28356350
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1236800.v1.1.491

Sequence Viewer

Length: 333 bp
ATGCAGGCAGTGAAGGACAAGATAAATGAAATGGGCGCCTTGCGCAAGGTCAAGGAGGAGGCCAGGGCCGAAGAGAGGGCTGAGAAGGAAATAGCAAAGGCGAGAGTAGACGTAGCTCATGAGGTGAGATTGGCTAAAGAAGCTGAAGCAGCAATGTCACTGCATGTGGCCATGGCTGGGGAGATTGCCAGAGCTGGAGACAAAGCAGCACATGAACATGAAATTAAGGCAATGCATACGGCTAATGGTACGAACACTGCACATGAACATGGAGCTTCTCCTGCTGAAATGGCTGGTGTGGGAGGTCATCCAGCTTATAAACGAATGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_1

Protein Analysis

111

Amino Acids

11.81

Weight (kDa)

6.71

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 64 7.7e-12 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017358)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08030
malus_domestica MD00G1098100.v1.1 MD02G1236800.v1.1
prunus_persica Prupe.2G109300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0341271
rosa_laevigata RLG00000029078
rosa_roxburghii Rroxscaffold_4G00311910
rosa_rugosa Rorug01G0158200.1
rosa_samantha Rh1AG172600 Rh1BG140700 Rh1CG160700 Rh1DG172900
rosa_wichuraiana Rw1G014400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
Acc16I TGCGCA 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 35
AccI GTMKAC 1 cut(s) 108
AcoI YGGCCR 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 165
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 2 cut(s) 75, 177
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 5 cut(s) 116, 143, 194, 275, 314
AluI AGCT 5 cut(s) 116, 143, 194, 275, 314
Alw26I GTCTC 1 cut(s) 192
AoxI GGCC 3 cut(s) 60, 66, 168
ApeKI GCWGC 2 cut(s) 149, 206
AspLEI GCGC 2 cut(s) 38, 45
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 136
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 161, 218
BceAI ACGGC 1 cut(s) 255
BciT130I CCWGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 2 cut(s) 150, 207
BlsI GCNGC 2 cut(s) 151, 208
Bme1390I CCNGG 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 63, 171
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 177
Bse3DI GCAATG 2 cut(s) 159, 237
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 63, 171
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 75, 177
BseMI GCAATG 2 cut(s) 159, 237
BseMII CTCAG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 161, 218
BseYI CCCAGC 1 cut(s) 176
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 3 cut(s) 62, 68, 170
BshNI GGYRCC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 75, 177
BsmAI GTCTC 1 cut(s) 192
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 3 cut(s) 62, 68, 170
Bsp19I CCATGG 1 cut(s) 171
BspANI GGCC 3 cut(s) 62, 68, 170
BspCNI CTCAG 1 cut(s) 73
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 159, 237
BssECI CCNNGG 2 cut(s) 63, 171
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 81
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 307
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 45
BstMAI GTCTC 1 cut(s) 192
BstMWI GCNNNNNNNGC 5 cut(s) 42, 140, 149, 281, 290
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 161, 218
BsuRI GGCC 3 cut(s) 62, 68, 170
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 3 cut(s) 15, 158, 255
BtsIMutI CAGTG 3 cut(s) 15, 158, 255
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 1 cut(s) 118
CfoI GCGC 2 cut(s) 38, 45
Cfr13I GGNCC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 249
CviAII CATG 7 cut(s) 119, 164, 172, 212, 218, 263, 269
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 1 cut(s) 81
DinI GGCGCC 1 cut(s) 37
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 171
EcoT22I ATGCAT 1 cut(s) 237
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 7 cut(s) 122, 167, 175, 215, 221, 266, 272
FaiI YATR 9 cut(s) 120, 165, 173, 213, 219, 237, 264, 270, 318
FatI CATG 7 cut(s) 118, 163, 171, 211, 217, 262, 268
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 2 cut(s) 150, 207
FokI GGATG 1 cut(s) 294
Fsp4HI GCNGC 2 cut(s) 150, 207
FspI TGCGCA 1 cut(s) 44
GlaI GCGC 2 cut(s) 37, 44
GluI GCNGC 2 cut(s) 150, 207
GsaI CCCAGC 1 cut(s) 180
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 3 cut(s) 62, 68, 170
HhaI GCGC 2 cut(s) 38, 45
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 7 cut(s) 122, 167, 175, 215, 221, 266, 272
Hin6I GCGC 2 cut(s) 36, 43
HinP1I GCGC 2 cut(s) 36, 43
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 109
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 7, 79
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 4 cut(s) 4, 163, 235, 260
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 140, 149, 281, 290
HpyF3I CTNAG 1 cut(s) 81
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 7 cut(s) 122, 167, 175, 215, 221, 266, 272
HspAI GCGC 2 cut(s) 36, 43
KasI GGCGCC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 8 cut(s) 49, 76, 162, 180, 202, 279, 294, 324
Lsp1109I GCAGC 2 cut(s) 161, 218
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 156
MboII GAAGA 1 cut(s) 83
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 1 cut(s) 222
MluNI TGGCCA 1 cut(s) 170
Mly113I GGCGCC 1 cut(s) 36
MnlI CCTC 5 cut(s) 49, 52, 69, 115, 296
Mox20I TGGCCA 1 cut(s) 170
Mph1103I ATGCAT 1 cut(s) 237
MscI TGGCCA 1 cut(s) 170
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 2 cut(s) 216, 267
Msp20I TGGCCA 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 64
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 5 cut(s) 42, 140, 149, 281, 290
NarI GGCGCC 1 cut(s) 36
NcoI CCATGG 1 cut(s) 171
NlaIII CATG 7 cut(s) 122, 167, 175, 215, 221, 266, 272
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 156
NsbI TGCGCA 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 1 cut(s) 167
PagI TCATGA 1 cut(s) 118
PctI GAATGC 1 cut(s) 330
PkrI GCNGC 2 cut(s) 151, 208
PluTI GGCGCC 1 cut(s) 39
PsiI TTATAA 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 62
PspFI CCCAGC 1 cut(s) 176
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 1 cut(s) 66
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
RseI CAYNNNNRTG 2 cut(s) 216, 267
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 150, 207
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 64
SetI ASST 9 cut(s) 51, 114, 118, 126, 145, 196, 277, 307, 316
SfoI GGCGCC 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 216, 267
Sse9I AATT 1 cut(s) 222
SspDI GGCGCC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 171
TaiI ACGT 1 cut(s) 114
TasI AATT 1 cut(s) 222
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 3 cut(s) 15, 165, 262
TseFI GTSAC 1 cut(s) 156
TseI GCWGC 2 cut(s) 149, 206
Tsp45I GTSAC 1 cut(s) 156
TspDTI ATGAA 4 cut(s) 42, 228, 234, 279
TspRI CASTG 3 cut(s) 15, 165, 262
XceI RCATGY 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.