Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0008427

Physical Location & Seq

Genomic Coordinates Forward (+)

27340090 .. 27353043

Gene Structure 12954 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1128300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGGATCTTCCTAGCCTCGCTGTGATACTACAGGCTGCTCTCAGTACCAATCCCGATGAGCGCAAAGCCGCCGAGCAGAGCCTCAATCAGTTTCAGTATACACCACAGCATTTGGTGAGAATGTTGCAAATTATAGTGGACAGCAATTGTGACATGGCTGTGCGGCAAGTTGGCAGCATCCATTTCAAGAATTTCATTGCAAAGAACTGGTCACCTATTGATCCTGATGATCAGCAGAAGATATCACAGAGTGATAAAGACGTCGTGAGGGATCACATTCTAGTCTTTGTCACACAGGTTCCCCCATTGTTAAGGGTACAGCTAGGTGAGTGCCTGAAGACAATTATTCATGCTGATTACCCTGAGCAGTGGCCACGCCTTTTAGACTGGGTGAAGCATAACTTACAAGATCAACAAGTCTATGGAGCTTTGTTTGTTTTGCGGATTCTCTCTAGAAAATATGAGTTCAAAACTGATGAGGAGAGGACACCAGTTTATCGCATTGTTGAGGAGACATTTCCACCTTTGCTTAATATATTCAACAGGCTTGTCCAAATACCCAACCCATCACTTGAAGTAGCAGATTTGATCAAGCTTATCTGTAAAATTTTCTGGTCATCCATATATCTAGAGATTCCAAAGCAACTATTTGATGGAAATGTGTTCAATGCTTGGATGATGCTTTTCTTAAACATATTGGAGAGACCTGTTCCTTCTGAAGGCCAGCCTTCAGATCCTGATCTTAGAAAAGCATGGGGGTGGTGGAAAGTGAAGAAATGGACTGTCCACATTTTAAATCGGCTGTACACTCGGTTTGGAGATTTAAAAATTCAACTGCCAGAAAACAAAGCATTTGCTCAAATGTTTCAGAAGAATTATGCAGGGAAGATTCTGGAGTGTCACTTAAATTTGTTGAATGTGATCCGAACTGGTGGCTACTTACCTGACAGAGTTATCAATCTCATTCTTCAGTATTTAAGCAATAGCATATCAAAGAATAGCATGTATAATCTGCTGCAACCTCGACTTAATGTTCTTCTTTTTGAGATTGTTTTTCCCCTTATGTGTTTCAATGACAATGATCTAAGGCTTTGGGATGAAGACCCGCATGAATATGTGAGGAAGGGTTATGATATCATTGAAGACTTATACAGTCCAAGGACGGCTTCCATGGATTTGGTGAGTGAGTTGGTTAGAAAACGTGGAAAGGAAAATCTTCATAAGTTCATTCAATTCATTGTGGAAATTTTTAAGAGGTATGATGAAGCACCAGTGGAATATAAGCCGTATCGACAGAAAGATGGTGCTCTTCTTGCTATTGGAGCACTTTGCGACAAACTGAAACAAACCGAACCCTACAAGTCTGAACTTGAGCATATGTTGGTGCAACATGTATTCCCTGAGTTTAGCAGTCCTGTTGGTCATCTTAGAGCTAAGGCAGCCTGGGTCGCGGGACAGTATGCTCATATTAACTTCTCGGATTCGAACAATTTCCGTAAAGCATTGCATAGTGTTGTTGAGGGGATGCGAGACCCGGAACTTCCTGTTCGAGTTGACTCAGTTTTTGCATTGCGCTCTTTTGTTGAAGCTTGCAGTGATTTGAATGAGATCCGGCCAATTCTTCCTCAGCTTCTTGATGAGTTTTTCAAGCTCATGAATGAGGTTGAGAATGAGGATCTTGTCTTTACTCTGGAGACAATAGTAGACAAGTTCGGGGAGGAAATGGCACCTTATGCTCTTGGTTTGTGCCAAAATCTGGCAGCTGCATTTTGGAGGTGTATGAATACTGCTGAAGCTGATGATGACGTTGATGACCCTGGTGCTTTGGCAGCAGTTGGTTGTTTGCGTGCTATTAGCACCATTCTTGAATCAGTGAGCCGGCTTCATCACCTTTTTGTCCAAGTTGAACCAACTTTGCTTCCTATAATGCGCAGGATGTTGACAACTGACGGTCAAGAGGTTTTTGAAGAAGTGTTGGAAATCATATCGTATATGACATTTTTCTCTCCAACAATATCTTTGGATATGTGGAGTCTTTGGCCATTAATGATGGACGCATTGGCAGAATGGGCTATTGATTTCTTCCCAAATATTCTGGTTCCTTTGGATAACTATATTTCCAGGGCGACTGCACAGTTCCTCACTTGTAAAGAACCAGACTACCAGCAAAGCCTTTGGAATATGATTGCAGCTATCATGTCGGATAAAAATATGGAAGACAGTGATATTGAGCCTGCTCCAAAGCTCATTCAAGTAGTTTTTCAGAACTGCAGAGGGCAGGTTGATCAATGGGTTGAACCATATCTTAGAATTACAGTTGAGCGGTTACGCCGATCAGAGAAGTCATACTTGAAATGTCTTCTCATTGAAGTGATTGCCGATGCACTATACTATAATGCAGCTTTGGCACTCAGCATATTGCAGAAGCTGGGTGTTGCAACAGAGATCTTTAATCTTTGGTTCCAGATGCTGCAACAGGTCAAGAAAAGTGGTGTGCGTGCCAATTTTAAGAGGGAACATGATAAAAAAGTATGCTGTCTGGGTTTGACATCATTAATGACACTCCCAGCTGAACAGTTGCCTGGGGAGTCATTGGGCCGTGTTTTCAGGGCTGCCCTTGATCTCCTGGTTGCGTACAAGGAGCAAGTTGCAGAAGCTGCAAAGGAGGAAGAACCAGAAGATGACAATGAAATGGATGATTTCCAAACTGATGATGATGATGAATATGGTGATGGATCTGACAAGGAAATGGGACTTGATACCGAGGATGGTGATGAAGCTGATAGCGTTAAACTTCAAAAGTTAGCTGCACAGTCAAAGTCTTTCCGGCCTAGTGATGAATACGACTCAGACTCGGACGATGATTTCAGTGATGATGAGGAGTTACAGTCACCAATTGATGATGTGGACCCTTTTATCTATTTTGTTGATGCAGTCAAAGGCATGCAAGCATCGGATCAGTTGAGGTTTCAGAGCCTTATGCAGACTCTGGACTTCCACTATCAGGCTCTTGCAAATGGTGTTGCACAGCATGCTGAACAGAGGAGAGCAGAAATTGAAAAAGAGAAGATGGTGAAGGCAGCAGCTGCCACAGCTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008565 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016441 GO:0016458 GO:0017038 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0023052 GO:0031047 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0035194 GO:0035195 GO:0035280 GO:0040029 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048519 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0070727 GO:0070922 GO:0071702 GO:0071705 GO:0071826 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko04013 map04013

Pfam Domains

Protein Families

Cse1 IBN_N

Protein Analysis

1034

Amino Acids

118.82

Weight (kDa)

4.81

Isoelectric Point (pI)

47.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	24 - 97	6.7e-16	Importin-beta N-terminal domain
Cse1	PF08506	153 - 450	1.4e-08	Cse1
TPR_IPO11	PF25758	632 - 902	1.3e-09	Importin-11-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31660 AT3G59020 AT3G59020
fragaria_vesca	FvH4_4g12200
malus_domestica	MD00G1128300.v1.1 MD04G1028800.v1.1
prunus_persica	Prupe.1G180500_v2.0.a1 Prupe.1G180500_v2.0.a1 Prupe.1G180500_v2.0.a1
pyrus_communis	pycom04g02510 pycom04g02520 pycom13g29470
rosa_chinensis	RchiOBHm_Chr4g0409961
rosa_laevigata	RLG00000008510
rosa_multiflora	Rmu_sc0000365.1_g000108 Rmu_sc0000987.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354450
rosa_rugosa	Rorug04G0099700
rosa_samantha	Rh4AG158700 Rh4BG158100 Rh4CG169500 Rh4DG153600
rosa_wichuraiana	Rw4G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	264
Acc16I	TGCGCA	1 cut(s)	1931
Acc36I	ACCTGC	1 cut(s)	2269
AccB1I	GGYRCC	1 cut(s)	1726
AccB7I	CCANNNNNTGG	1 cut(s)	1756
AccBSI	CCGCTC	1 cut(s)	2323
AccI	GTMKAC	2 cut(s)	98, 1704
AccII	CGCG	1 cut(s)	1451
AciI	CCGC	6 cut(s)	69, 163, 442, 1106, 1451, 2323
AclWI	GGATC	9 cut(s)	12, 215, 279, 728, 916, 1603, 1683, 2743, 2964
AcoI	YGGCCR	3 cut(s)	371, 1613, 2039
AcsI	RAATTY	5 cut(s)	190, 606, 828, 907, 1245
AcuI	CTGAAG	5 cut(s)	356, 714, 738, 953, 1812
AcyI	GRCGYC	1 cut(s)	261
AdeI	CACNNNGTG	1 cut(s)	251
AfaI	GTAC	4 cut(s)	46, 318, 806, 2636
AfiI	CCNNNNNNNGG	3 cut(s)	719, 1756, 3004
AflIII	ACRYGT	1 cut(s)	1390
AjnI	CCWGG	5 cut(s)	1442, 1816, 2120, 2581, 2625
AjuI	GAANNNNNNNTTGG	2 cut(s)	1150, 1182
Alw21I	GWGCWC	2 cut(s)	1309, 1327
Alw26I	GTCTC	4 cut(s)	506, 697, 1524, 1688
AlwI	GGATC	9 cut(s)	12, 215, 279, 728, 916, 1603, 1683, 2743, 2964
AlwNI	CAGNNNCTG	6 cut(s)	737, 2428, 2470, 2657, 2989, 3086
AoxI	GGCC	6 cut(s)	371, 721, 1613, 2039, 2596, 2828
ApoI	RAATTY	5 cut(s)	190, 606, 828, 907, 1245
ArsI	GACNNNNNNTTYG	4 cut(s)	194, 226, 1547, 1579
AseI	ATTAAT	2 cut(s)	2045, 2555
Asp700I	GAANNNNTTC	2 cut(s)	1215, 1490
AspLEI	GCGC	3 cut(s)	63, 1575, 1932
AspS9I	GGNCC	2 cut(s)	2596, 2908
AsuC2I	CCSGG	1 cut(s)	1535
AsuII	TTCGAA	1 cut(s)	1484
AvaII	GGWCC	1 cut(s)	2908
BalI	TGGCCA	2 cut(s)	373, 2041
BanI	GGYRCC	1 cut(s)	1726
BbsI	GAAGAC	5 cut(s)	344, 1107, 1149, 2223, 2351
Bbv12I	GWGCWC	2 cut(s)	1309, 1327
BbvCI	CCTCAGC	1 cut(s)	1626
BccI	CCATC	7 cut(s)	574, 647, 1295, 2044, 2726, 2762, 3064
BceAI	ACGGC	3 cut(s)	1179, 1270, 2583
BciT130I	CCWGG	5 cut(s)	1444, 1818, 2122, 2583, 2627
BclI	TGATCA	3 cut(s)	229, 588, 2284
BcnI	CCSGG	1 cut(s)	1535
BcoDI	GTCTC	4 cut(s)	506, 697, 1524, 1688
BfaI	CTAG	6 cut(s)	12, 281, 323, 453, 629, 2832
BfmI	CTRYAG	2 cut(s)	29, 2269
BfuAI	ACCTGC	1 cut(s)	2269
BglII	AGATCT	1 cut(s)	2445
Bme1390I	CCNGG	6 cut(s)	1444, 1535, 1818, 2122, 2583, 2627
Bme18I	GGWCC	1 cut(s)	2908
BmgT120I	GGNCC	2 cut(s)	2596, 2908
BmiI	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
BmrFI	CCNGG	6 cut(s)	1444, 1535, 1818, 2122, 2583, 2627
BmrI	ACTGGG	1 cut(s)	397
BmsI	GCATC	7 cut(s)	186, 669, 1515, 2371, 2457, 2920, 2960
BmuI	ACTGGG	1 cut(s)	397
BpiI	GAAGAC	5 cut(s)	344, 1107, 1149, 2223, 2351
BpmI	CTGGAG	2 cut(s)	914, 1712
Bpu10I	CCTNAGC	3 cut(s)	363, 1434, 1626
Bpu14I	TTCGAA	1 cut(s)	1484
BpuEI	CTTGAG	1 cut(s)	1391
BpuMI	CCSGG	1 cut(s)	1535
BsaBI	GATNNNNATC	1 cut(s)	738
BsaHI	GRCGYC	1 cut(s)	261
BsaI	GGTCTC	2 cut(s)	697, 1524
BsaJI	CCNNGG	7 cut(s)	1157, 1170, 1443, 1816, 2121, 2582, 2763
BsaXI	ACNNNNNCTCC	2 cut(s)	2873, 2903
Bsc4I	CCNNNNNNNGG	3 cut(s)	719, 1756, 3004
Bse118I	RCCGGY	1 cut(s)	1878
Bse1I	ACTGG	5 cut(s)	212, 392, 491, 934, 1271
Bse3DI	GCAATG	3 cut(s)	195, 1502, 1568
Bse8I	GATNNNNATC	1 cut(s)	738
BseBI	CCWGG	5 cut(s)	1444, 1818, 2122, 2583, 2627
BseDI	CCNNGG	7 cut(s)	1157, 1170, 1443, 1816, 2121, 2582, 2763
BseGI	GGATG	8 cut(s)	177, 617, 681, 1102, 1530, 1941, 2701, 2773
BseJI	GATNNNNATC	1 cut(s)	738
BseLI	CCNNNNNNNGG	3 cut(s)	719, 1756, 3004
BseMI	GCAATG	3 cut(s)	195, 1502, 1568
BseMII	CTCAG	7 cut(s)	55, 354, 1392, 1572, 1640, 2425, 2862
BseNI	ACTGG	5 cut(s)	212, 392, 491, 934, 1271
BseRI	GAGGAG	4 cut(s)	494, 524, 2894, 3058
BseYI	CCCAGC	2 cut(s)	2428, 2566
BsgI	GTGCAG	2 cut(s)	2115, 2793
Bsh1236I	CGCG	1 cut(s)	1451
BshFI	GGCC	6 cut(s)	373, 723, 1615, 2041, 2598, 2830
BshNI	GGYRCC	1 cut(s)	1726
BsiHKAI	GWGCWC	2 cut(s)	1309, 1327
BsiSI	CCGG	4 cut(s)	1535, 1612, 1879, 2827
BslFI	GGGAC	2 cut(s)	1467, 2766
BslI	CCNNNNNNNGG	3 cut(s)	719, 1756, 3004
BsmAI	GTCTC	4 cut(s)	506, 697, 1524, 1688
BsmFI	GGGAC	2 cut(s)	1467, 2766
BsnI	GGCC	6 cut(s)	373, 723, 1615, 2041, 2598, 2830
Bso31I	GGTCTC	2 cut(s)	697, 1524
Bsp119I	TTCGAA	1 cut(s)	1484
Bsp1286I	GDGCHC	2 cut(s)	1309, 1327
Bsp1407I	TGTACA	1 cut(s)	804
Bsp19I	CCATGG	1 cut(s)	1170
BspACI	CCGC	6 cut(s)	69, 163, 442, 1106, 1451, 2323
BspANI	GGCC	6 cut(s)	373, 723, 1615, 2041, 2598, 2830
BspCNI	CTCAG	7 cut(s)	54, 355, 1393, 1571, 1639, 2424, 2861
BspFNI	CGCG	1 cut(s)	1451
BspHI	TCATGA	2 cut(s)	1653, 3098
BspLI	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
BspMAI	CTGCAG	1 cut(s)	2273
BspMI	ACCTGC	1 cut(s)	2269
BspPI	GGATC	9 cut(s)	12, 215, 279, 728, 916, 1603, 1683, 2743, 2964
BspQI	GCTCTTC	1 cut(s)	1314
BspT104I	TTCGAA	1 cut(s)	1484
BspT107I	GGYRCC	1 cut(s)	1726
BspTNI	GGTCTC	2 cut(s)	697, 1524
BsrBI	CCGCTC	1 cut(s)	2323
BsrDI	GCAATG	3 cut(s)	195, 1502, 1568
BsrFI	RCCGGY	1 cut(s)	1878
BsrGI	TGTACA	1 cut(s)	804
BsrI	ACTGG	5 cut(s)	212, 392, 491, 934, 1271
BssAI	RCCGGY	1 cut(s)	1878
BssECI	CCNNGG	7 cut(s)	1157, 1170, 1443, 1816, 2121, 2582, 2763
BssNAI	GTATAC	1 cut(s)	99
BssNI	GRCGYC	1 cut(s)	261
BssT1I	CCWWGG	2 cut(s)	1157, 1170
Bst1107I	GTATAC	1 cut(s)	99
Bst2UI	CCWGG	5 cut(s)	1444, 1818, 2122, 2583, 2627
Bst6I	CTCTTC	1 cut(s)	1314
BstACI	GRCGYC	1 cut(s)	261
BstAPI	GCANNNNNTGC	4 cut(s)	1733, 2657, 3032, 3086
BstAUI	TGTACA	1 cut(s)	804
BstBI	TTCGAA	1 cut(s)	1484
BstC8I	GCNNGC	9 cut(s)	725, 1591, 1848, 1880, 2235, 2499, 2945, 2949, 3033
BstDSI	CCRYGG	1 cut(s)	1170
BstEII	GGTNACC	1 cut(s)	210
BstENI	CCTNNNNNAGG	1 cut(s)	717
BstF5I	GGATG	8 cut(s)	177, 617, 681, 1102, 1530, 1941, 2701, 2773
BstFNI	CGCG	1 cut(s)	1451
BstHHI	GCGC	3 cut(s)	63, 1575, 1932
BstMAI	GTCTC	4 cut(s)	506, 697, 1524, 1688
BstNI	CCWGG	5 cut(s)	1444, 1818, 2122, 2583, 2627
BstNSI	RCATGY	4 cut(s)	1006, 1394, 2947, 3035
BstPI	GGTNACC	1 cut(s)	210
BstSCI	CCNGG	6 cut(s)	1442, 1533, 1816, 2120, 2581, 2625
BstSFI	CTRYAG	2 cut(s)	29, 2269
BstUI	CGCG	1 cut(s)	1451
BstV2I	GAAGAC	5 cut(s)	344, 1107, 1149, 2223, 2351
BstX2I	RGATCY	6 cut(s)	4, 733, 1608, 1675, 2445, 2735
BstXI	CCANNNNNNTGG	1 cut(s)	1177
BstYI	RGATCY	6 cut(s)	4, 733, 1608, 1675, 2445, 2735
BstZ17I	GTATAC	1 cut(s)	99
BsuRI	GGCC	6 cut(s)	373, 723, 1615, 2041, 2598, 2830
BtgI	CCRYGG	1 cut(s)	1170
BtsCI	GGATG	8 cut(s)	177, 617, 681, 1102, 1530, 1941, 2701, 2773
BtsI	GCAGTG	2 cut(s)	374, 1600
BtsIMutI	CAGTG	6 cut(s)	374, 1278, 1600, 1878, 2227, 2875
BveI	ACCTGC	1 cut(s)	2269
Cac8I	GCNNGC	9 cut(s)	725, 1591, 1848, 1880, 2235, 2499, 2945, 2949, 3033
CaiI	CAGNNNCTG	6 cut(s)	737, 2428, 2470, 2657, 2989, 3086
CciI	TCATGA	2 cut(s)	1653, 3098
CfoI	GCGC	3 cut(s)	63, 1575, 1932
Cfr10I	RCCGGY	1 cut(s)	1878
Cfr13I	GGNCC	2 cut(s)	2596, 2908
CseI	GACGC	1 cut(s)	2063
Csp6I	GTAC	4 cut(s)	45, 317, 805, 2635
CspCI	CAANNNNNGTGG	4 cut(s)	93, 128, 2470, 2505
CviQI	GTAC	4 cut(s)	45, 317, 805, 2635
DraI	TTTAAA	2 cut(s)	795, 825
DraIII	CACNNNGTG	1 cut(s)	251
EaeI	YGGCCR	3 cut(s)	371, 1613, 2039
Eam1104I	CTCTTC	1 cut(s)	1314
EarI	CTCTTC	1 cut(s)	1314
Eco130I	CCWWGG	2 cut(s)	1157, 1170
Eco31I	GGTCTC	2 cut(s)	697, 1524
Eco32I	GATATC	2 cut(s)	243, 1135
Eco47I	GGWCC	1 cut(s)	2908
Eco57I	CTGAAG	5 cut(s)	356, 714, 738, 953, 1812
Eco91I	GGTNACC	1 cut(s)	210
EcoNI	CCTNNNNNAGG	1 cut(s)	717
EcoO65I	GGTNACC	1 cut(s)	210
EcoRII	CCWGG	5 cut(s)	1442, 1816, 2120, 2581, 2625
EcoRV	GATATC	2 cut(s)	243, 1135
EcoT14I	CCWWGG	2 cut(s)	1157, 1170
ErhI	CCWWGG	2 cut(s)	1157, 1170
FalI	AAGNNNNNCTT	6 cut(s)	386, 418, 1150, 1182, 2333, 2365
FaqI	GGGAC	2 cut(s)	1467, 2766
FauI	CCCGC	2 cut(s)	1113, 1444
FauNDI	CATATG	1 cut(s)	1377
FbaI	TGATCA	3 cut(s)	229, 588, 2284
FblI	GTMKAC	2 cut(s)	98, 1704
FokI	GGATG	8 cut(s)	164, 604, 688, 1109, 1537, 1948, 2708, 2780
FspBI	CTAG	6 cut(s)	12, 281, 323, 453, 629, 2832
FspI	TGCGCA	1 cut(s)	1931
GlaI	GCGC	3 cut(s)	62, 1574, 1931
GsaI	CCCAGC	2 cut(s)	2432, 2570
GsuI	CTGGAG	2 cut(s)	914, 1712
HaeIII	GGCC	6 cut(s)	373, 723, 1615, 2041, 2598, 2830
HapII	CCGG	4 cut(s)	1535, 1612, 1879, 2827
HgaI	GACGC	1 cut(s)	2063
HhaI	GCGC	3 cut(s)	63, 1575, 1932
Hin1I	GRCGYC	1 cut(s)	261
Hin6I	GCGC	3 cut(s)	61, 1573, 1930
HinP1I	GCGC	3 cut(s)	61, 1573, 1930
HincII	GTYRAC	2 cut(s)	1555, 1941
HindII	GTYRAC	2 cut(s)	1555, 1941
HindIII	AAGCTT	2 cut(s)	593, 1587
HpaII	CCGG	4 cut(s)	1535, 1612, 1879, 2827
Hpy166II	GTNNAC	8 cut(s)	99, 139, 787, 807, 1555, 1705, 1941, 2908
Hpy8I	GTNNAC	8 cut(s)	99, 139, 787, 807, 1555, 1705, 1941, 2908
Hpy99I	CGWCG	1 cut(s)	266
HpyAV	CCTTC	5 cut(s)	713, 723, 738, 1117, 3070
HpyCH4IV	ACGT	3 cut(s)	261, 1201, 1806
HpySE526I	ACGT	3 cut(s)	261, 1201, 1806
Hsp92I	GRCGYC	1 cut(s)	261
HspAI	GCGC	3 cut(s)	61, 1573, 1930
KroI	GCCGGC	1 cut(s)	1878
KroNI	GCCGGC	1 cut(s)	1880
Ksp22I	TGATCA	3 cut(s)	229, 588, 2284
LguI	GCTCTTC	1 cut(s)	1314
LmnI	GCTCC	4 cut(s)	425, 1322, 2242, 2641
LweI	GCATC	7 cut(s)	186, 669, 1515, 2371, 2457, 2920, 2960
MaeI	CTAG	6 cut(s)	12, 281, 323, 453, 629, 2832
MaeII	ACGT	3 cut(s)	261, 1201, 1806
MaeIII	GTNAC	7 cut(s)	149, 210, 289, 899, 2325, 2883, 2889
MbiI	CCGCTC	1 cut(s)	2323
MfeI	CAATTG	2 cut(s)	145, 2895
MflI	RGATCY	6 cut(s)	4, 733, 1608, 1675, 2445, 2735
MhlI	GDGCHC	2 cut(s)	1309, 1327
MlsI	TGGCCA	2 cut(s)	373, 2041
MluNI	TGGCCA	2 cut(s)	373, 2041
MlyI	GAGTC	6 cut(s)	1550, 2041, 2597, 2840, 2846, 2980
MmeI	TCCRAC	3 cut(s)	1956, 2033, 2181
Mox20I	TGGCCA	2 cut(s)	373, 2041
MroNI	GCCGGC	1 cut(s)	1878
MroXI	GAANNNNTTC	2 cut(s)	1215, 1490
MscI	TGGCCA	2 cut(s)	373, 2041
MslI	CAYNNNNRTG	4 cut(s)	158, 757, 1113, 2369
Msp20I	TGGCCA	2 cut(s)	373, 2041
MspA1I	CMGCKG	3 cut(s)	1763, 2570, 3086
MspI	CCGG	4 cut(s)	1535, 1612, 1879, 2827
MspR9I	CCNGG	6 cut(s)	1444, 1535, 1818, 2122, 2583, 2627
MunI	CAATTG	2 cut(s)	145, 2895
MvaI	CCWGG	5 cut(s)	1444, 1818, 2122, 2583, 2627
MvnI	CGCG	1 cut(s)	1451
NaeI	GCCGGC	1 cut(s)	1880
NciI	CCSGG	1 cut(s)	1535
NcoI	CCATGG	1 cut(s)	1170
NdeI	CATATG	1 cut(s)	1377
NgoMIV	GCCGGC	1 cut(s)	1878
NlaIV	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
NmeAIII	GCCGAG	1 cut(s)	97
NmuCI	GTSAC	5 cut(s)	149, 210, 289, 899, 2889
NsbI	TGCGCA	1 cut(s)	1931
NspI	RCATGY	4 cut(s)	1006, 1394, 2947, 3035
NspV	TTCGAA	1 cut(s)	1484
PaeI	GCATGC	2 cut(s)	2947, 3035
PagI	TCATGA	2 cut(s)	1653, 3098
PciI	ACATGT	1 cut(s)	1390
PciSI	GCTCTTC	1 cut(s)	1314
PdiI	GCCGGC	1 cut(s)	1880
PdmI	GAANNNNTTC	2 cut(s)	1215, 1490
PfeI	GAWTC	5 cut(s)	445, 634, 889, 1481, 1868
PflMI	CCANNNNNTGG	1 cut(s)	1756
PleI	GAGTC	6 cut(s)	1550, 2040, 2596, 2840, 2846, 2980
PpsI	GAGTC	6 cut(s)	1550, 2040, 2596, 2840, 2846, 2980
PscI	ACATGT	1 cut(s)	1390
PshBI	ATTAAT	2 cut(s)	2045, 2555
Psp6I	CCWGG	5 cut(s)	1442, 1816, 2120, 2581, 2625
PspEI	GGTNACC	1 cut(s)	210
PspFI	CCCAGC	2 cut(s)	2428, 2566
PspGI	CCWGG	5 cut(s)	1442, 1816, 2120, 2581, 2625
PspN4I	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
PspPI	GGNCC	2 cut(s)	2596, 2908
PstI	CTGCAG	1 cut(s)	2273
PstNI	CAGNNNCTG	6 cut(s)	737, 2428, 2470, 2657, 2989, 3086
PsuI	RGATCY	6 cut(s)	4, 733, 1608, 1675, 2445, 2735
PvuII	CAGCTG	3 cut(s)	1763, 2570, 3086
RsaI	GTAC	4 cut(s)	46, 318, 806, 2636
RsaNI	GTAC	4 cut(s)	45, 317, 805, 2635
RseI	CAYNNNNRTG	4 cut(s)	158, 757, 1113, 2369
SapI	GCTCTTC	1 cut(s)	1314
Sau96I	GGNCC	2 cut(s)	2596, 2908
SchI	GAGTC	6 cut(s)	1550, 2041, 2597, 2840, 2846, 2980
ScrFI	CCNGG	6 cut(s)	1444, 1535, 1818, 2122, 2583, 2627
SduI	GDGCHC	2 cut(s)	1309, 1327
SfaNI	GCATC	7 cut(s)	186, 669, 1515, 2371, 2457, 2920, 2960
SfcI	CTRYAG	2 cut(s)	29, 2269
SfuI	TTCGAA	1 cut(s)	1484
SinI	GGWCC	1 cut(s)	2908
SmiMI	CAYNNNNRTG	4 cut(s)	158, 757, 1113, 2369
SmlI	CTYRAG	1 cut(s)	1370
SmoI	CTYRAG	1 cut(s)	1370
SphI	GCATGC	2 cut(s)	2947, 3035
SsiI	CCGC	6 cut(s)	69, 163, 442, 1106, 1451, 2323
SspI	AATATT	1 cut(s)	2092
SspMI	CTAG	6 cut(s)	12, 281, 323, 453, 629, 2832
StyD4I	CCNGG	6 cut(s)	1442, 1533, 1816, 2120, 2581, 2625
StyI	CCWWGG	2 cut(s)	1157, 1170
TaiI	ACGT	3 cut(s)	264, 1204, 1809
TaqI	TCGA	4 cut(s)	1024, 1291, 1484, 1549
TatI	WGTACW	1 cut(s)	804
TauI	GCSGC	2 cut(s)	71, 166
TfiI	GAWTC	5 cut(s)	445, 634, 889, 1481, 1868
TscAI	CASTG	6 cut(s)	374, 1278, 1600, 1878, 2227, 2875
TseFI	GTSAC	5 cut(s)	149, 210, 289, 899, 2889
Tsp45I	GTSAC	5 cut(s)	149, 210, 289, 899, 2889
TspGWI	ACGGA	1 cut(s)	1484
TspRI	CASTG	6 cut(s)	374, 1278, 1600, 1878, 2227, 2875
Van91I	CCANNNNNTGG	1 cut(s)	1756
VpaK11BI	GGWCC	1 cut(s)	2908
VspI	ATTAAT	2 cut(s)	2045, 2555
XagI	CCTNNNNNAGG	1 cut(s)	717
XapI	RAATTY	5 cut(s)	190, 606, 828, 907, 1245
XbaI	TCTAGA	2 cut(s)	452, 628
XceI	RCATGY	4 cut(s)	1006, 1394, 2947, 3035
XmiI	GTMKAC	2 cut(s)	98, 1704
XmnI	GAANNNNTTC	2 cut(s)	1215, 1490
XspI	CTAG	6 cut(s)	12, 281, 323, 453, 629, 2832
ZraI	GACGTC	1 cut(s)	262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.