Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008427

Physical Location & Seq

Genomic Coordinates Forward (+)

3251954 .. 3264348

Gene Structure 12395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1028800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGGATCTTCCCAGCCTCGCTGTAATACTACAGGCTGCTCTCAGTACCAATCCCGATGAGCGGAAAGCCGCCGAGCAGAGCCTCAATCAGTTTCAGTATACACCACAGCATTTGGTGAGAATGTTGCAAATTATAGTGGACAGCAATTGTGACATGGCTGTGCGGCAAGTTGGCAGCATCCATTTCAAGAATTTCATTGCAAAGAACTGGTCACCTCTTGATCCTGATGTGCAGCAGAAGATATCACAGAGTGATAAAGACGTGGTGAGGGATCACATTCTAGTCTTTGTCACACAGGTTCCCCCATTATTAAGGGTACAGCTAGGTGAGTGCCTGAAGACAATTATTCATGCTGATTACCCCGAGCAGTGGCCACGCCTTTTAGACTGGGTGAAGCATAACTTACAAGATCAACAAGTCTATGGAGCTTTGTTTGTTTTGCGGATTCTCTCTAGAAAATATGAGTTCAAATCTGATGAGGAGAGGACACCGGTTTATCGCATTGTTGAGGAAACATTCCCAGCTCTGCTAAATATATTCAATAGGCTTGTCCAAATTCCCAACCCATCACTGGAAGTAGCAGATTTGATCAAGCTTATCTGTAAAATTTTCTGGTCATCCATATATCTGGAGATTCCAAAGCAACTATTTGATGCAAACGTGTTTAATGCTTGGATGATGCTTTTCTTAAACATATTGGAGAGACCTGTTCCTTCTGAAGGCCAGCCTTCAGATCCCGATCTTAGAAAAGCATGGGGGTGGTGGAAAGTGAAGAAATGGACTGTTCACATTTTGAATCGCCTGTACACTCGGTTTGGAGATTTAAAAATTCAACAACCAGAAAACAAAGCATTTGCTCAAATGTTTCAGAAGAATTATGCAGGGAAGATTCTGGAGTGTCACTTAAATTTGTTGAATGTGATCCGAACTGGTGGCTACTTACCTGACAGAGTTATCAATCTTGTTCTTCAGTATTTAAGCAATAGTATCTCGAAGAATAGCATGTATAATCTGATGCAACCTCGACTTAATGTTCTTCTTTTCGAGATTGTTTTTCCCCTTATGTGTTTCAATGATAATGATCTAAGGCTTTGGGATGAAGACCCACATGAATATGTGAGAAAGGGTTATGATATCATTGAAGACTTGTATAGTCCAAGGACAGCTTCCATGGATTTTGTGAGTGAGTTGGTTAGAAAACGTGGAAAGGAAAATCTTCACAAGTTCATTCAATTCATTGTGGAGGTTTTTAAGAGGTATGACGAAGCACCAGTGGAATATAAGCCATATCGACAAAAAGATGGTGCTCTTCTTGCTATTGGATCACTTTGCGACAAACTGAAACAAACTGAACCCTACAAGTCTGAACTTGAGCGTATGTTGGTGCAACATGTATTCCCCGAGTTTAGCAGTCCTGTTGGCCATCTTAGAGCTAAGGCAGCCTGGGTTGCGGGACAGTATGCTCATATTAACTTCTCGGATTCGAACAATTTTCGTAAGGCATTGCATAGTGTTGTCTCGGGGATGCGAGATCCAGAACTTCCTGTTCGAGTTGACTCAGTATTTGCATTACGCTCTTTTGTTGAAGCTTGCAGTGATTTGAATGAGATCCGGCCAATTCTTCCGCAGCTTCTTGATGAGTTTTTCAAGCTCATGAATGAGGTTGAAAATGAGGATCTTGTCTTTACTCTGGAGACAATAGTGGACAAGTTCGGGGAGGAAATGGCACCTTATGCTCTTGGTCTGTGCCAAAATCTGGCAGCTGCATTTTGGAGGTGTATGAATACAGCTGAAGCTGATGATGATGTTGATGATCCTGGTGCTTTGGCAGCAGTTGGTTGTTTGCGTGCTATTAGCACCATTCTTGAGTCAGTGAGCCGGCTTCCTCACCTTTTCGTTCAAGTTGAACCAACTTTGCTTCCTATAATGCGCAGGATGTTGACAACTGATGGCCAAGAGGTTTTTGAAGAAGTGTTGGAAATTGTATCATATATGACATTTTTCTCTCCAACGATATCTTTAGATATGTGGAGTCTCTGGCCATTGATGATGGAAGCATTGGCAGAATGGGCTATTGATTTCTTTCCAAATATTCTGGTTCCTTTGGATAACTACATATCCAGGGGGACAGCACAGTTCCTCACTTGTAAAGAACCAGACTACCAGCAAGGCCTTTGGAATATGATTGCAGCTATCATGGCAGATAAAAATATGGAAGACGGTGATATTGAGCCTGCTCCAAAGCTCATTCAAGTAGTTTTTCAGAACTGCAGAGGGCAGGTTGATCAATGGGTTGAACCGTACCTTAGAATTACAGTTGAGCGGTTACGCCGATCTGAGAAGCCGTACTTGAAATGCCTTCTCATTGAAGTGATTGCGGATGCACTGTACTATAACGCAGCTTTGGCGCTCAGCATATTGCAGAACCTGGGTGTTGCAACAGAGATCTTTAATCTTTGGTTCCAGATGCTGCAACAGGTCAAGAAAAGTGGTGTGCGTGCCAATTTTAAGAGGGAACATGACAAAAAAGTATGCTGTCTGGGTTTGACATCATTAATGACACTCCCAGCTGAACTGTTGCCTGGGGAGGCATTGGGCCGTGTTTTCAGGGCTGCCCTTGATCTCCTAGTTGCGTACAAGGAACAAGTTGCAGAAGCTGCAAAGGAGGAAGAACCAGAAGATGACGATGAAATGGATGGTTTCCCAACTGATGATGATGATGAATATGGTGATGGATCTGACAAGGAAATGGGACTTGATGCTGAGGATGGTGATGAAGTTGATAGCGTTAAACTTCAAAAGTTAGCTGCACAGGCAAAGTCTTTCCGCCCTAGTGATGAATATGACTCAGACTCGGACGATGATTTCAGTGATGATGAGGAATTACAGTCACCAATTGATGATGTGGACCCTTTTATCCTTTTTGTTGATGCAGTCAAGGCCATGCAAGCATCAGATCCGTTGAGAGTTCACAGCCTTATGCAGACTCTGGACTTCCAGTATCAGGCTCTTGCAAATGGCGTTGCACAGCATGCTGAACAGAGGAGAGCAGATATTGAAAAAGAGAAGATGGAGAAGGCAGCAGCTGCCACAGCTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008565 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016441 GO:0016458 GO:0017038 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0023052 GO:0031047 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0035194 GO:0035195 GO:0035280 GO:0040029 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048519 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0070727 GO:0070922 GO:0071702 GO:0071705 GO:0071826 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko04013 map04013

Pfam Domains

Protein Families

Cse1 IBN_N

Protein Analysis

1034

Amino Acids

118.44

Weight (kDa)

4.8

Isoelectric Point (pI)

44.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	24 - 97	8e-16	Importin-beta N-terminal domain
Cse1	PF08506	154 - 450	5.7e-09	Cse1
TPR_IPO11	PF25758	634 - 934	4e-08	Importin-11-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31660 AT3G59020 AT3G59020
fragaria_vesca	FvH4_4g12200
malus_domestica	MD00G1128300.v1.1 MD04G1028800.v1.1
prunus_persica	Prupe.1G180500_v2.0.a1 Prupe.1G180500_v2.0.a1 Prupe.1G180500_v2.0.a1
pyrus_communis	pycom04g02510 pycom04g02520 pycom13g29470
rosa_chinensis	RchiOBHm_Chr4g0409961
rosa_laevigata	RLG00000008510
rosa_multiflora	Rmu_sc0000365.1_g000108 Rmu_sc0000987.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354450
rosa_rugosa	Rorug04G0099700
rosa_samantha	Rh4AG158700 Rh4BG158100 Rh4CG169500 Rh4DG153600
rosa_wichuraiana	Rw4G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1931
Acc36I	ACCTGC	1 cut(s)	2269
AccB1I	GGYRCC	1 cut(s)	1726
AccB7I	CCANNNNNTGG	1 cut(s)	1756
AccBSI	CCGCTC	2 cut(s)	61, 2323
AccI	GTMKAC	1 cut(s)	98
AciI	CCGC	9 cut(s)	61, 69, 163, 442, 1451, 1625, 2323, 2378, 2827
AcoI	YGGCCR	5 cut(s)	371, 1420, 1613, 1951, 2039
AcsI	RAATTY	5 cut(s)	190, 555, 606, 828, 907
AcuI	CTGAAG	5 cut(s)	356, 714, 738, 953, 1812
AdeI	CACNNNGTG	1 cut(s)	251
AfaI	GTAC	7 cut(s)	46, 318, 806, 2303, 2348, 2390, 2636
AfiI	CCNNNNNNNGG	6 cut(s)	60, 369, 571, 719, 1756, 3004
AflIII	ACRYGT	2 cut(s)	660, 1390
AgeI	ACCGGT	1 cut(s)	490
AjiI	CACGTC	1 cut(s)	262
AjnI	CCWGG	5 cut(s)	1442, 1816, 2120, 2427, 2581
AjuI	GAANNNNNNNTTGG	2 cut(s)	1150, 1182
Alw21I	GWGCWC	1 cut(s)	1309
Alw26I	GTCTC	4 cut(s)	697, 1522, 1688, 2039
AlwNI	CAGNNNCTG	5 cut(s)	2428, 2470, 2657, 2989, 3086
Ama87I	CYCGRG	3 cut(s)	362, 1400, 1519
AoxI	GGCC	9 cut(s)	371, 721, 1420, 1613, 1951, 2039, 2170, 2596, 2940
ApoI	RAATTY	5 cut(s)	190, 555, 606, 828, 907
ArsI	GACNNNNNNTTYG	4 cut(s)	194, 226, 1547, 1579
AseI	ATTAAT	1 cut(s)	2555
AsiGI	ACCGGT	1 cut(s)	490
Asp700I	GAANNNNTTC	1 cut(s)	1215
AspLEI	GCGC	2 cut(s)	1932, 2410
AspS9I	GGNCC	2 cut(s)	2596, 2908
AsuII	TTCGAA	1 cut(s)	1484
AvaI	CYCGRG	3 cut(s)	362, 1400, 1519
AvaII	GGWCC	1 cut(s)	2908
BalI	TGGCCA	4 cut(s)	373, 1422, 1953, 2041
BanI	GGYRCC	1 cut(s)	1726
BbsI	GAAGAC	4 cut(s)	344, 1107, 1149, 2223
Bbv12I	GWGCWC	1 cut(s)	1309
BbvCI	CCTCAGC	1 cut(s)	2763
BccI	CCATC	9 cut(s)	574, 1295, 1431, 1943, 2044, 2690, 2726, 2762, 3064
BceAI	ACGGC	2 cut(s)	2329, 2583
BciT130I	CCWGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
BclI	TGATCA	2 cut(s)	588, 2284
BcoDI	GTCTC	4 cut(s)	697, 1522, 1688, 2039
BfaI	CTAG	5 cut(s)	281, 323, 453, 2627, 2832
BfmI	CTRYAG	2 cut(s)	29, 2269
BfoI	RGCGCY	1 cut(s)	2411
BfuAI	ACCTGC	1 cut(s)	2269
BglII	AGATCT	1 cut(s)	2445
BlpI	GCTNAGC	1 cut(s)	2411
Bme1390I	CCNGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
Bme18I	GGWCC	1 cut(s)	2908
BmeT110I	CYCGRG	3 cut(s)	362, 1400, 1519
BmgBI	CACGTC	1 cut(s)	262
BmgT120I	GGNCC	2 cut(s)	2596, 2908
BmiI	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
BmrFI	CCNGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
BmrI	ACTGGG	1 cut(s)	397
BmuI	ACTGGG	1 cut(s)	397
BpiI	GAAGAC	4 cut(s)	344, 1107, 1149, 2223
BpmI	CTGGAG	3 cut(s)	650, 914, 1712
Bpu10I	CCTNAGC	2 cut(s)	1434, 2763
Bpu1102I	GCTNAGC	1 cut(s)	2411
Bpu14I	TTCGAA	1 cut(s)	1484
BpuEI	CTTGAG	2 cut(s)	1391, 1886
BsaBI	GATNNNNATC	2 cut(s)	738, 1080
BsaI	GGTCTC	1 cut(s)	697
BsaJI	CCNNGG	6 cut(s)	1157, 1170, 1443, 2121, 2428, 2582
BsaWI	WCCGGW	1 cut(s)	490
Bsc4I	CCNNNNNNNGG	6 cut(s)	60, 369, 571, 719, 1756, 3004
Bse118I	RCCGGY	2 cut(s)	490, 1878
Bse1I	ACTGG	6 cut(s)	212, 392, 576, 934, 1271, 2998
Bse3DI	GCAATG	2 cut(s)	195, 1502
Bse8I	GATNNNNATC	2 cut(s)	738, 1080
BseBI	CCWGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
BseDI	CCNNGG	6 cut(s)	1157, 1170, 1443, 2121, 2428, 2582
BseGI	GGATG	9 cut(s)	177, 617, 681, 1102, 1530, 1941, 2386, 2701, 2773
BseJI	GATNNNNATC	2 cut(s)	738, 1080
BseLI	CCNNNNNNNGG	6 cut(s)	60, 369, 571, 719, 1756, 3004
BseMI	GCAATG	2 cut(s)	195, 1502
BseMII	CTCAG	6 cut(s)	55, 1572, 2328, 2425, 2754, 2862
BseNI	ACTGG	6 cut(s)	212, 392, 576, 934, 1271, 2998
BseRI	GAGGAG	2 cut(s)	494, 3058
BseYI	CCCAGC	3 cut(s)	11, 520, 2566
BsgI	GTGCAG	2 cut(s)	251, 2793
BshFI	GGCC	9 cut(s)	373, 723, 1422, 1615, 1953, 2041, 2172, 2598, 2942
BshNI	GGYRCC	1 cut(s)	1726
BshTI	ACCGGT	1 cut(s)	490
BsiHKAI	GWGCWC	1 cut(s)	1309
BsiHKCI	CYCGRG	3 cut(s)	362, 1400, 1519
BsiSI	CCGG	3 cut(s)	491, 1612, 1879
BslFI	GGGAC	3 cut(s)	1467, 2140, 2766
BslI	CCNNNNNNNGG	6 cut(s)	60, 369, 571, 719, 1756, 3004
BsmAI	GTCTC	4 cut(s)	697, 1522, 1688, 2039
BsmFI	GGGAC	3 cut(s)	1467, 2140, 2766
BsnI	GGCC	9 cut(s)	373, 723, 1422, 1615, 1953, 2041, 2172, 2598, 2942
Bso31I	GGTCTC	1 cut(s)	697
BsoBI	CYCGRG	3 cut(s)	362, 1400, 1519
Bsp119I	TTCGAA	1 cut(s)	1484
Bsp1286I	GDGCHC	1 cut(s)	1309
Bsp1407I	TGTACA	1 cut(s)	804
Bsp1720I	GCTNAGC	1 cut(s)	2411
Bsp19I	CCATGG	1 cut(s)	1170
BspACI	CCGC	9 cut(s)	61, 69, 163, 442, 1451, 1625, 2323, 2378, 2827
BspANI	GGCC	9 cut(s)	373, 723, 1422, 1615, 1953, 2041, 2172, 2598, 2942
BspCNI	CTCAG	6 cut(s)	54, 1571, 2329, 2424, 2755, 2861
BspHI	TCATGA	2 cut(s)	1653, 3098
BspLI	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
BspMAI	CTGCAG	1 cut(s)	2273
BspMI	ACCTGC	1 cut(s)	2269
BspQI	GCTCTTC	1 cut(s)	1314
BspT104I	TTCGAA	1 cut(s)	1484
BspT107I	GGYRCC	1 cut(s)	1726
BspTNI	GGTCTC	1 cut(s)	697
BsrBI	CCGCTC	2 cut(s)	61, 2323
BsrDI	GCAATG	2 cut(s)	195, 1502
BsrFI	RCCGGY	2 cut(s)	490, 1878
BsrGI	TGTACA	1 cut(s)	804
BsrI	ACTGG	6 cut(s)	212, 392, 576, 934, 1271, 2998
BssAI	RCCGGY	2 cut(s)	490, 1878
BssECI	CCNNGG	6 cut(s)	1157, 1170, 1443, 2121, 2428, 2582
BssNAI	GTATAC	1 cut(s)	99
BssT1I	CCWWGG	2 cut(s)	1157, 1170
Bst1107I	GTATAC	1 cut(s)	99
Bst2UI	CCWGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
Bst4CI	ACNGT	9 cut(s)	784, 1458, 2136, 2222, 2301, 2317, 2388, 2577, 2889
Bst6I	CTCTTC	1 cut(s)	1314
BstAPI	GCANNNNNTGC	4 cut(s)	1733, 2657, 3032, 3086
BstAUI	TGTACA	1 cut(s)	804
BstBI	TTCGAA	1 cut(s)	1484
BstC8I	GCNNGC	8 cut(s)	725, 1591, 1848, 1880, 2235, 2499, 2949, 3033
BstDSI	CCRYGG	1 cut(s)	1170
BstEII	GGTNACC	1 cut(s)	210
BstENI	CCTNNNNNAGG	1 cut(s)	717
BstF5I	GGATG	9 cut(s)	177, 617, 681, 1102, 1530, 1941, 2386, 2701, 2773
BstH2I	RGCGCY	1 cut(s)	2411
BstHHI	GCGC	2 cut(s)	1932, 2410
BstMAI	GTCTC	4 cut(s)	697, 1522, 1688, 2039
BstNI	CCWGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
BstNSI	RCATGY	3 cut(s)	1006, 1394, 3035
BstPI	GGTNACC	1 cut(s)	210
BstSCI	CCNGG	5 cut(s)	1442, 1816, 2120, 2427, 2581
BstSFI	CTRYAG	2 cut(s)	29, 2269
BstV2I	GAAGAC	4 cut(s)	344, 1107, 1149, 2223
BstX2I	RGATCY	8 cut(s)	4, 733, 1531, 1608, 1675, 2445, 2735, 2956
BstXI	CCANNNNNNTGG	1 cut(s)	628
BstYI	RGATCY	8 cut(s)	4, 733, 1531, 1608, 1675, 2445, 2735, 2956
BstZ17I	GTATAC	1 cut(s)	99
BsuRI	GGCC	9 cut(s)	373, 723, 1422, 1615, 1953, 2041, 2172, 2598, 2942
BtgI	CCRYGG	1 cut(s)	1170
BtrI	CACGTC	1 cut(s)	262
BtsCI	GGATG	9 cut(s)	177, 617, 681, 1102, 1530, 1941, 2386, 2701, 2773
BtsI	GCAGTG	2 cut(s)	374, 1600
BtsIMutI	CAGTG	7 cut(s)	374, 569, 1278, 1600, 1878, 2384, 2875
BveI	ACCTGC	1 cut(s)	2269
Cac8I	GCNNGC	8 cut(s)	725, 1591, 1848, 1880, 2235, 2499, 2949, 3033
CaiI	CAGNNNCTG	5 cut(s)	2428, 2470, 2657, 2989, 3086
CciI	TCATGA	2 cut(s)	1653, 3098
CfoI	GCGC	2 cut(s)	1932, 2410
Cfr10I	RCCGGY	2 cut(s)	490, 1878
Cfr13I	GGNCC	2 cut(s)	2596, 2908
Csp6I	GTAC	7 cut(s)	45, 317, 805, 2302, 2347, 2389, 2635
CspAI	ACCGGT	1 cut(s)	490
CspCI	CAANNNNNGTGG	4 cut(s)	93, 128, 2470, 2505
CviQI	GTAC	7 cut(s)	45, 317, 805, 2302, 2347, 2389, 2635
DraI	TTTAAA	1 cut(s)	825
DraIII	CACNNNGTG	1 cut(s)	251
EaeI	YGGCCR	5 cut(s)	371, 1420, 1613, 1951, 2039
Eam1104I	CTCTTC	1 cut(s)	1314
EarI	CTCTTC	1 cut(s)	1314
EciI	GGCGGA	1 cut(s)	2816
Eco130I	CCWWGG	2 cut(s)	1157, 1170
Eco147I	AGGCCT	1 cut(s)	2172
Eco31I	GGTCTC	1 cut(s)	697
Eco32I	GATATC	3 cut(s)	243, 1135, 2016
Eco47I	GGWCC	1 cut(s)	2908
Eco57I	CTGAAG	5 cut(s)	356, 714, 738, 953, 1812
Eco88I	CYCGRG	3 cut(s)	362, 1400, 1519
Eco91I	GGTNACC	1 cut(s)	210
EcoNI	CCTNNNNNAGG	1 cut(s)	717
EcoO65I	GGTNACC	1 cut(s)	210
EcoRII	CCWGG	5 cut(s)	1442, 1816, 2120, 2427, 2581
EcoRV	GATATC	3 cut(s)	243, 1135, 2016
EcoT14I	CCWWGG	2 cut(s)	1157, 1170
ErhI	CCWWGG	2 cut(s)	1157, 1170
FalI	AAGNNNNNCTT	6 cut(s)	386, 418, 1150, 1182, 2333, 2365
FaqI	GGGAC	3 cut(s)	1467, 2140, 2766
FauI	CCCGC	1 cut(s)	1444
FbaI	TGATCA	2 cut(s)	588, 2284
FblI	GTMKAC	1 cut(s)	98
FokI	GGATG	9 cut(s)	164, 604, 688, 1109, 1537, 1948, 2393, 2708, 2780
FspBI	CTAG	5 cut(s)	281, 323, 453, 2627, 2832
FspI	TGCGCA	1 cut(s)	1931
GlaI	GCGC	2 cut(s)	1931, 2409
GsaI	CCCAGC	3 cut(s)	15, 524, 2570
GsuI	CTGGAG	3 cut(s)	650, 914, 1712
HaeII	RGCGCY	1 cut(s)	2411
HaeIII	GGCC	9 cut(s)	373, 723, 1422, 1615, 1953, 2041, 2172, 2598, 2942
HapII	CCGG	3 cut(s)	491, 1612, 1879
HhaI	GCGC	2 cut(s)	1932, 2410
Hin6I	GCGC	2 cut(s)	1930, 2408
HinP1I	GCGC	2 cut(s)	1930, 2408
HincII	GTYRAC	2 cut(s)	1555, 1941
HindII	GTYRAC	2 cut(s)	1555, 1941
HindIII	AAGCTT	2 cut(s)	593, 1587
HpaII	CCGG	3 cut(s)	491, 1612, 1879
Hpy166II	GTNNAC	9 cut(s)	99, 139, 787, 807, 1555, 1705, 1941, 2908, 2971
Hpy8I	GTNNAC	9 cut(s)	99, 139, 787, 807, 1555, 1705, 1941, 2908, 2971
HpyAV	CCTTC	5 cut(s)	713, 723, 738, 2369, 3070
HpyCH4III	ACNGT	9 cut(s)	784, 1458, 2136, 2222, 2301, 2317, 2388, 2577, 2889
HpyCH4IV	ACGT	3 cut(s)	261, 660, 1201
HpySE526I	ACGT	3 cut(s)	261, 660, 1201
HspAI	GCGC	2 cut(s)	1930, 2408
KroI	GCCGGC	1 cut(s)	1878
KroNI	GCCGGC	1 cut(s)	1880
Ksp22I	TGATCA	2 cut(s)	588, 2284
LguI	GCTCTTC	1 cut(s)	1314
LmnI	GCTCC	2 cut(s)	425, 2242
MaeI	CTAG	5 cut(s)	281, 323, 453, 2627, 2832
MaeII	ACGT	3 cut(s)	261, 660, 1201
MaeIII	GTNAC	6 cut(s)	149, 210, 289, 899, 2325, 2889
MbiI	CCGCTC	2 cut(s)	61, 2323
MfeI	CAATTG	2 cut(s)	145, 2895
MflI	RGATCY	8 cut(s)	4, 733, 1531, 1608, 1675, 2445, 2735, 2956
MhlI	GDGCHC	1 cut(s)	1309
MlsI	TGGCCA	4 cut(s)	373, 1422, 1953, 2041
MluNI	TGGCCA	4 cut(s)	373, 1422, 1953, 2041
MlyI	GAGTC	6 cut(s)	1550, 1877, 2041, 2840, 2846, 2980
MmeI	TCCRAC	2 cut(s)	1956, 2033
Mox20I	TGGCCA	4 cut(s)	373, 1422, 1953, 2041
MroNI	GCCGGC	1 cut(s)	1878
MroXI	GAANNNNTTC	1 cut(s)	1215
MscI	TGGCCA	4 cut(s)	373, 1422, 1953, 2041
MslI	CAYNNNNRTG	4 cut(s)	158, 757, 1113, 2369
Msp20I	TGGCCA	4 cut(s)	373, 1422, 1953, 2041
MspA1I	CMGCKG	4 cut(s)	1763, 1790, 2570, 3086
MspI	CCGG	3 cut(s)	491, 1612, 1879
MspR9I	CCNGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
MunI	CAATTG	2 cut(s)	145, 2895
MvaI	CCWGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
NaeI	GCCGGC	1 cut(s)	1880
NcoI	CCATGG	1 cut(s)	1170
NgoMIV	GCCGGC	1 cut(s)	1878
NlaIV	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
NmeAIII	GCCGAG	1 cut(s)	97
NmuCI	GTSAC	5 cut(s)	149, 210, 289, 899, 2889
NsbI	TGCGCA	1 cut(s)	1931
NspI	RCATGY	3 cut(s)	1006, 1394, 3035
NspV	TTCGAA	1 cut(s)	1484
PaeI	GCATGC	1 cut(s)	3035
PagI	TCATGA	2 cut(s)	1653, 3098
PceI	AGGCCT	1 cut(s)	2172
PciI	ACATGT	1 cut(s)	1390
PciSI	GCTCTTC	1 cut(s)	1314
PdiI	GCCGGC	1 cut(s)	1880
PdmI	GAANNNNTTC	1 cut(s)	1215
PfeI	GAWTC	5 cut(s)	445, 634, 796, 889, 1481
PflMI	CCANNNNNTGG	1 cut(s)	1756
PinAI	ACCGGT	1 cut(s)	490
PleI	GAGTC	6 cut(s)	1550, 1876, 2040, 2840, 2846, 2980
PpsI	GAGTC	6 cut(s)	1550, 1876, 2040, 2840, 2846, 2980
PscI	ACATGT	1 cut(s)	1390
PshBI	ATTAAT	1 cut(s)	2555
Psp6I	CCWGG	5 cut(s)	1442, 1816, 2120, 2427, 2581
PspEI	GGTNACC	1 cut(s)	210
PspFI	CCCAGC	3 cut(s)	11, 520, 2566
PspGI	CCWGG	5 cut(s)	1442, 1816, 2120, 2427, 2581
PspN4I	GGNNCC	5 cut(s)	300, 1728, 2100, 2462, 2910
PspPI	GGNCC	2 cut(s)	2596, 2908
PstI	CTGCAG	1 cut(s)	2273
PstNI	CAGNNNCTG	5 cut(s)	2428, 2470, 2657, 2989, 3086
PsuI	RGATCY	8 cut(s)	4, 733, 1531, 1608, 1675, 2445, 2735, 2956
PvuII	CAGCTG	4 cut(s)	1763, 1790, 2570, 3086
RsaI	GTAC	7 cut(s)	46, 318, 806, 2303, 2348, 2390, 2636
RsaNI	GTAC	7 cut(s)	45, 317, 805, 2302, 2347, 2389, 2635
RseI	CAYNNNNRTG	4 cut(s)	158, 757, 1113, 2369
SapI	GCTCTTC	1 cut(s)	1314
Sau96I	GGNCC	2 cut(s)	2596, 2908
SchI	GAGTC	6 cut(s)	1550, 1877, 2041, 2840, 2846, 2980
ScrFI	CCNGG	5 cut(s)	1444, 1818, 2122, 2429, 2583
SduI	GDGCHC	1 cut(s)	1309
SfcI	CTRYAG	2 cut(s)	29, 2269
SfuI	TTCGAA	1 cut(s)	1484
SinI	GGWCC	1 cut(s)	2908
SmiMI	CAYNNNNRTG	4 cut(s)	158, 757, 1113, 2369
SmlI	CTYRAG	2 cut(s)	1370, 1865
SmoI	CTYRAG	2 cut(s)	1370, 1865
SphI	GCATGC	1 cut(s)	3035
SseBI	AGGCCT	1 cut(s)	2172
SsiI	CCGC	9 cut(s)	61, 69, 163, 442, 1451, 1625, 2323, 2378, 2827
SspI	AATATT	1 cut(s)	2092
SspMI	CTAG	5 cut(s)	281, 323, 453, 2627, 2832
StuI	AGGCCT	1 cut(s)	2172
StyD4I	CCNGG	5 cut(s)	1442, 1816, 2120, 2427, 2581
StyI	CCWWGG	2 cut(s)	1157, 1170
TaaI	ACNGT	9 cut(s)	784, 1458, 2136, 2222, 2301, 2317, 2388, 2577, 2889
TaiI	ACGT	3 cut(s)	264, 663, 1204
TaqI	TCGA	6 cut(s)	992, 1024, 1044, 1291, 1484, 1549
TatI	WGTACW	2 cut(s)	804, 2388
TauI	GCSGC	2 cut(s)	71, 166
TfiI	GAWTC	5 cut(s)	445, 634, 796, 889, 1481
TscAI	CASTG	7 cut(s)	374, 576, 1278, 1600, 1878, 2391, 2875
TseFI	GTSAC	5 cut(s)	149, 210, 289, 899, 2889
Tsp45I	GTSAC	5 cut(s)	149, 210, 289, 899, 2889
TspGWI	ACGGA	1 cut(s)	2949
TspRI	CASTG	7 cut(s)	374, 576, 1278, 1600, 1878, 2391, 2875
Van91I	CCANNNNNTGG	1 cut(s)	1756
VpaK11BI	GGWCC	1 cut(s)	2908
VspI	ATTAAT	1 cut(s)	2555
XagI	CCTNNNNNAGG	1 cut(s)	717
XapI	RAATTY	5 cut(s)	190, 555, 606, 828, 907
XbaI	TCTAGA	1 cut(s)	452
XceI	RCATGY	3 cut(s)	1006, 1394, 3035
XcmI	CCANNNNNNNNNTGG	1 cut(s)	568
XmiI	GTMKAC	1 cut(s)	98
XmnI	GAANNNNTTC	1 cut(s)	1215
XspI	CTAG	5 cut(s)	281, 323, 453, 2627, 2832

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.