Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0012444

Physical Location & Seq

Genomic Coordinates Forward (+)

28218807 .. 28223357

Gene Structure 4551 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1130900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGGCTTCCATTGGGTCTCTGCCTTGTATAATAGAGACCAAGACTGACAGCTCTGTGCTTGTTCATAGCATTATTAATCGCCGGCTCAGATTCCCATTCTTGACGAATCGTCCAAGAAGCTCCATTGAAGTTAGTGGGATCAATGCTTCTGCAGGTGGGTCTTCAGAAGCTGTGGGAATGTGCAGAGGACAGAATGATTGGTGCTCAAGAGGGAATCTTTGGAGGATTAAGCCGCTTCATGCGATTGGAAAGAGCTCCGCGCAAGGTGAGGACGGCGGCGAGCCGGAGGATCCCCTTCAGGCCACCATTGAAAAGAGCAAGAAGGTTCTTGCTACGCAAAGAGAACTGCTTCAACAGATTGCTGAAAGAAGGAAATTGTTATCTTCTATACAAAGTGGTGATATAAGCCAAGAAGAAGAGGAAGCTTCTTTTGAACAGGGGAACGAATCGTTTTCGAATGCAAAAAGTTCTTCAAGTAGTGGTGATGATACGGTTGAAGACCAAATTGGCAGCATTGCTTCGAGCGGCTATGAGCATTCAACTGAAGAAAAGGAGTTCGAAACTGTGCCTTCTGCTCTTAGTAGAGGGCTTGATGAAATAGAAAAGGATCATGGAGACGCTTTACCTTTTAATAAGTCTCCCATTGAGCTAGAGTCCAGTAAGCATGTGAGTACGGATAGTTCCAAGACAGAGTGGTCTGATGGTTTGCCATCTTTTCTTACAAACCTTGAGTCGTCAACGCTAAAAGATGAGGAGCTTGTTGATCTGAAAGAACCAAGTGTAGAGGAGGTCCATGAGGGGGCAGCTGGTTTTACCAGTGAAGACGTAAAGCCGCCTCCTTTGGCGGGGCCTAATGTCATGAATGTCATATTAGTAGCTGTAGAATGCGCTCCATGGTCGAAAACAGGTGGGCTTGGAGATGTTGCTGGGGCTTTGCCAAAGGCATTGGCTCGGCGTGGACACAGGGTCATGGTTGTGGCACCTCTCTATGGTGATTATGCGGAACCCCAACATTCAGGAGTTCGGAAAGTATATAAAGTTGCTGGCCAGGATATGGAGGTAGCATATTTCCAAGCCTATATTGATGGTGTAGATTTTGTCTTCATTGAATCTGCTGTGTTCCGTCACATGGAGAATAATATATATGCAGGAAAGCGTGAGGATATTTTAAAACGCATGGTGTTATTTTGCAAGGCAGCAGTTGACGTCCCTTGGCTTGTCCCCTGTGGTGGTGCTTGTTATGGTGATGGAAACTTGGCTTTCATTGCAAATGATTGGCATACTGCATTGTTGCCGGTGTATTTGAAGGCATATTATCGAGACAATGGCTTAATGATATATACGAGGTCCATCCTTGTAATCCATAACATAGCTCACCAGGGTCGGGGGCCAGTAGCTGATTTCTCCTTCGTTGATCTTCCTGGACACTACCTGGACCTTTTCAAATTGTATGACCCTATTGGAGGTGAGCACTTCAATATCTTTGCAGCTGGTCTAAAGACAGCAGACCGTGTGGTTACTGTAAGTCATGGATATTCGCGGGAGCTTAAAACTGTTGAAGGTGGTTGGGGATTACACGGGATCATAAATGAGAATGACTGGAAATTCAGAGGCATTGTTAATGGAATTGACACGAAAGACTGGAACCCGCAGCATGATGCTTTCTTGAAATCAGATGGTTACACGAACTACTCCCTGGAGACACTTAAGACTGGCAAGGCTCAGTGTAAGGCGGCATTACAGAAGGAGCTGGGTTTACCACTCCGTGAGGATGTCCCAGTAATTGGTTTCATTGGGAGGCTGGATCACCAGAAGGGTGTCGATCTCATAGCAGAGGCAATTCCGTGGATGATGGGTCAGGATGTGCAACTAGTCATGTTAGGCTCTGGGAGACCTGACCTGGAAGAGATGCTCAGGGCATTCGAACACCAACACCGAGACAAGGTCAGGGGATGGGTTGGGTTTTCTGTACAAACAGCTCACAGGATAACTGCTGGTGCAGACATTTTGCTCATGCCGTCAAGATTTGAGCCGTGTGGACTGAACCAACTATACGCTATGAACTATGGGACAGTTCCAGTTGTTCATGCAGTTGGTGGACTGAGAGATACCGTGCATCCTTTCAATCCGTACGAGGAGTCGGGACTTGGATGGACATTTGACAGCGCTGAGGTCGGTGAGCTAATCCATGCATTAGGGAACTGCTTATATACTTATCGAGAGTACAAGCAGAGCTGGGAAGGAATCCAGAGACGAGGGATGATGCAAGACCTGAGTTGGGACCATGCTGCTCAGAATTATGAGGAGGTTCTTGTGGCTGCAAAGTACCAATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009011 GO:0009058 GO:0009059 GO:0009250 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

Glyco_trans_1_4 Glyco_transf_5 Glycos_transf_1

Protein Analysis

779

Amino Acids

85.91

Weight (kDa)

5.25

Isoelectric Point (pI)

42.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_5	PF08323	288 - 530	6.9e-56	Starch synthase catalytic domain
Glycos_transf_1	PF00534	588 - 709	1.6e-15	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	593 - 731	1.3e-16	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012444)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01180
fragaria_vesca	FvH4_6g48410 FvH4_6g48410 FvH4_6g48410
malus_domestica	MD00G1130900.v1.1 MD09G1052000.v1.1
prunus_persica	Prupe.3G266700_v2.0.a1 Prupe.3G266700_v2.0.a1
pyrus_communis	pycom111g04330
rosa_chinensis	RchiOBHm_Chr2g0168331
rosa_laevigata	RLG00000021767
rosa_multiflora	Rmu_sc0003354.1_g000016
rosa_roxburghii	Rroxscaffold_2G00083140
rosa_rugosa	Rorug02G0532700
rosa_samantha	Rh2AG602000 Rh2BG615300 Rh2CG583000 Rh2DG625500
rosa_wichuraiana	Rw2G049970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	143
AasI	GACNNNNNNGTC	1 cut(s)	694
AatII	GACGTC	1 cut(s)	1209
Acc36I	ACCTGC	1 cut(s)	143
AccB1I	GGYRCC	1 cut(s)	979
AccB7I	CCANNNNNTGG	2 cut(s)	1054, 1784
AccBSI	CCGCTC	1 cut(s)	525
AccII	CGCG	2 cut(s)	260, 1540
AclWI	GGATC	6 cut(s)	146, 284, 297, 615, 1589, 1812
AcoI	YGGCCR	1 cut(s)	1045
AcsI	RAATTY	1 cut(s)	1604
AcuI	CTGAAG	3 cut(s)	147, 281, 564
AcyI	GRCGYC	1 cut(s)	1206
AdeI	CACNNNGTG	1 cut(s)	1766
AfaI	GTAC	5 cut(s)	673, 1971, 2132, 2225, 2327
AfeI	AGCGCT	1 cut(s)	2167
AflII	CTTAAG	1 cut(s)	1706
AhdI	GACNNNNNGTC	2 cut(s)	108, 965
AhlI	ACTAGT	1 cut(s)	1870
AjnI	CCWGG	6 cut(s)	1047, 1377, 1420, 1431, 1695, 1899
AjuI	GAANNNNNNNTTGG	2 cut(s)	489, 521
Alw21I	GWGCWC	3 cut(s)	206, 257, 1473
Alw26I	GTCTC	9 cut(s)	21, 29, 609, 642, 1314, 1694, 1885, 1932, 2245
AlwI	GGATC	6 cut(s)	146, 284, 297, 615, 1589, 1812
AlwNI	CAGNNNCTG	2 cut(s)	170, 1397
Aor51HI	AGCGCT	1 cut(s)	2167
AoxI	GGCC	4 cut(s)	300, 848, 1045, 1388
ApeKI	GCWGC	7 cut(s)	510, 803, 1196, 1487, 1651, 2288, 2318
ApoI	RAATTY	1 cut(s)	1604
ArsI	GACNNNNNNTTYG	2 cut(s)	716, 748
AseI	ATTAAT	1 cut(s)	75
Asp700I	GAANNNNTTC	1 cut(s)	348
AspLEI	GCGC	3 cut(s)	262, 890, 2168
AspS9I	GGNCC	6 cut(s)	790, 848, 1347, 1388, 1435, 2281
AsuHPI	GGTGA	9 cut(s)	278, 410, 494, 1004, 1256, 1367, 1478, 1799, 2189
AsuII	TTCGAA	3 cut(s)	455, 558, 1923
AvaII	GGWCC	4 cut(s)	790, 1347, 1435, 2281
BalI	TGGCCA	1 cut(s)	1047
BamHI	GGATCC	1 cut(s)	289
BanI	GGYRCC	1 cut(s)	979
BanII	GRGCYC	1 cut(s)	257
BbsI	GAAGAC	4 cut(s)	153, 504, 828, 1093
Bbv12I	GWGCWC	3 cut(s)	206, 257, 1473
BbvCI	CCTCAGC	1 cut(s)	2169
BbvI	GCAGC	7 cut(s)	522, 815, 1208, 1499, 1663, 2275, 2305
BccI	CCATC	9 cut(s)	695, 718, 1079, 1241, 1358, 1670, 1846, 1947, 2145
BceAI	ACGGC	3 cut(s)	289, 2002, 2017
BciT130I	CCWGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
BcoDI	GTCTC	9 cut(s)	21, 29, 609, 642, 1314, 1694, 1885, 1932, 2245
BcuI	ACTAGT	1 cut(s)	1870
BfaI	CTAG	2 cut(s)	650, 1871
BfmI	CTRYAG	2 cut(s)	150, 879
BfoI	RGCGCY	1 cut(s)	2169
BfrI	CTTAAG	1 cut(s)	1706
BfuAI	ACCTGC	1 cut(s)	143
Bme1390I	CCNGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
Bme18I	GGWCC	4 cut(s)	790, 1347, 1435, 2281
BmeRI	GACNNNNNGTC	2 cut(s)	108, 965
BmgT120I	GGNCC	6 cut(s)	790, 848, 1347, 1388, 1435, 2281
BmiI	GGNNCC	7 cut(s)	291, 849, 981, 1005, 1389, 1646, 2282
BmrFI	CCNGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
BmrI	ACTGGG	1 cut(s)	1772
BmsI	GCATC	4 cut(s)	1648, 1899, 2125, 2253
BmuI	ACTGGG	1 cut(s)	1772
BpiI	GAAGAC	4 cut(s)	153, 504, 828, 1093
BpmI	CTGGAG	1 cut(s)	1718
Bpu10I	CCTNAGC	2 cut(s)	1913, 2169
Bpu14I	TTCGAA	3 cut(s)	455, 558, 1923
BpuEI	CTTGAG	2 cut(s)	190, 749
BsaHI	GRCGYC	1 cut(s)	1206
BsaI	GGTCTC	3 cut(s)	21, 29, 1885
BsaJI	CCNNGG	5 cut(s)	893, 1211, 1378, 1695, 1844
Bse118I	RCCGGY	2 cut(s)	81, 1294
Bse1I	ACTGG	8 cut(s)	657, 816, 1391, 1604, 1646, 1717, 1778, 2078
Bse3DI	GCAATG	2 cut(s)	513, 1263
BseBI	CCWGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
BseDI	CCNNGG	5 cut(s)	893, 1211, 1378, 1695, 1844
BseGI	GGATG	8 cut(s)	1350, 1777, 1854, 1867, 1958, 2116, 2156, 2265
BseMI	GCAATG	2 cut(s)	513, 1263
BseMII	CTCAG	7 cut(s)	100, 1736, 1927, 2093, 2160, 2264, 2306
BseNI	ACTGG	8 cut(s)	657, 816, 1391, 1604, 1646, 1717, 1778, 2078
BseRI	GAGGAG	4 cut(s)	767, 800, 2150, 2318
BseXI	GCAGC	7 cut(s)	522, 815, 1208, 1499, 1663, 2275, 2305
BseYI	CCCAGC	3 cut(s)	926, 1750, 2235
BsgI	GTGCAG	2 cut(s)	202, 2019
Bsh1236I	CGCG	2 cut(s)	260, 1540
BshFI	GGCC	4 cut(s)	302, 850, 1047, 1390
BshNI	GGYRCC	1 cut(s)	979
BsiHKAI	GWGCWC	3 cut(s)	206, 257, 1473
BsiSI	CCGG	3 cut(s)	82, 284, 1295
BsiWI	CGTACG	1 cut(s)	2130
BslFI	GGGAC	6 cut(s)	1193, 1205, 1760, 2083, 2157, 2294
BsmAI	GTCTC	9 cut(s)	21, 29, 609, 642, 1314, 1694, 1885, 1932, 2245
BsmBI	CGTCTC	2 cut(s)	609, 2245
BsmFI	GGGAC	6 cut(s)	1193, 1205, 1760, 2083, 2157, 2294
BsmI	GAATGC	4 cut(s)	463, 535, 890, 1919
BsnI	GGCC	4 cut(s)	302, 850, 1047, 1390
Bso31I	GGTCTC	3 cut(s)	21, 29, 1885
Bsp119I	TTCGAA	3 cut(s)	455, 558, 1923
Bsp1286I	GDGCHC	3 cut(s)	206, 257, 1473
Bsp1407I	TGTACA	1 cut(s)	1969
Bsp143I	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
Bsp19I	CCATGG	1 cut(s)	893
BspANI	GGCC	4 cut(s)	302, 850, 1047, 1390
BspCNI	CTCAG	7 cut(s)	99, 1735, 1926, 2094, 2161, 2265, 2305
BspFNI	CGCG	2 cut(s)	260, 1540
BspHI	TCATGA	1 cut(s)	858
BspLI	GGNNCC	7 cut(s)	291, 849, 981, 1005, 1389, 1646, 2282
BspMAI	CTGCAG	1 cut(s)	154
BspMI	ACCTGC	1 cut(s)	143
BspPI	GGATC	6 cut(s)	146, 284, 297, 615, 1589, 1812
BspT104I	TTCGAA	3 cut(s)	455, 558, 1923
BspT107I	GGYRCC	1 cut(s)	979
BspTI	CTTAAG	1 cut(s)	1706
BspTNI	GGTCTC	3 cut(s)	21, 29, 1885
BsrBI	CCGCTC	1 cut(s)	525
BsrDI	GCAATG	2 cut(s)	513, 1263
BsrFI	RCCGGY	2 cut(s)	81, 1294
BsrGI	TGTACA	1 cut(s)	1969
BsrI	ACTGG	8 cut(s)	657, 816, 1391, 1604, 1646, 1717, 1778, 2078
BssAI	RCCGGY	2 cut(s)	81, 1294
BssECI	CCNNGG	5 cut(s)	893, 1211, 1378, 1695, 1844
BssMI	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
BssNI	GRCGYC	1 cut(s)	1206
BssT1I	CCWWGG	2 cut(s)	893, 1211
Bst2UI	CCWGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
Bst4CI	ACNGT	7 cut(s)	493, 565, 1511, 1522, 1555, 2074, 2113
Bst6I	CTCTTC	2 cut(s)	411, 1899
BstACI	GRCGYC	1 cut(s)	1206
BstAFI	CTTAAG	1 cut(s)	1706
BstAUI	TGTACA	1 cut(s)	1969
BstBI	TTCGAA	3 cut(s)	455, 558, 1923
BstC8I	GCNNGC	3 cut(s)	83, 281, 1045
BstDEI	CTNAG	8 cut(s)	86, 578, 1722, 1913, 2102, 2169, 2273, 2292
BstDSI	CCRYGG	2 cut(s)	893, 1844
BstENI	CCTNNNNNAGG	1 cut(s)	1461
BstF5I	GGATG	8 cut(s)	1350, 1777, 1854, 1867, 1958, 2116, 2156, 2265
BstFNI	CGCG	2 cut(s)	260, 1540
BstH2I	RGCGCY	1 cut(s)	2169
BstHHI	GCGC	3 cut(s)	262, 890, 2168
BstKTI	GATC	8 cut(s)	141, 292, 610, 766, 1417, 1584, 1807, 1825
BstMAI	GTCTC	9 cut(s)	21, 29, 609, 642, 1314, 1694, 1885, 1932, 2245
BstMBI	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
BstMWI	GCNNNNNNNGC	1 cut(s)	1265
BstNI	CCWGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
BstNSI	RCATGY	1 cut(s)	668
BstSCI	CCNGG	6 cut(s)	1047, 1377, 1420, 1431, 1695, 1899
BstSFI	CTRYAG	2 cut(s)	150, 879
BstUI	CGCG	2 cut(s)	260, 1540
BstV1I	GCAGC	7 cut(s)	522, 815, 1208, 1499, 1663, 2275, 2305
BstV2I	GAAGAC	4 cut(s)	153, 504, 828, 1093
BstX2I	RGATCY	1 cut(s)	289
BstYI	RGATCY	1 cut(s)	289
BsuRI	GGCC	4 cut(s)	302, 850, 1047, 1390
BtgI	CCRYGG	2 cut(s)	893, 1844
BtsCI	GGATG	8 cut(s)	1350, 1777, 1854, 1867, 1958, 2116, 2156, 2265
BtsIMutI	CAGTG	2 cut(s)	823, 1730
BveI	ACCTGC	1 cut(s)	143
Cac8I	GCNNGC	3 cut(s)	83, 281, 1045
CaiI	CAGNNNCTG	2 cut(s)	170, 1397
CciI	TCATGA	1 cut(s)	858
CfoI	GCGC	3 cut(s)	262, 890, 2168
Cfr10I	RCCGGY	2 cut(s)	81, 1294
Cfr13I	GGNCC	6 cut(s)	790, 848, 1347, 1388, 1435, 2281
CseI	GACGC	1 cut(s)	626
Csp6I	GTAC	5 cut(s)	672, 1970, 2131, 2224, 2326
CviQI	GTAC	5 cut(s)	672, 1970, 2131, 2224, 2326
DdeI	CTNAG	8 cut(s)	86, 578, 1722, 1913, 2102, 2169, 2273, 2292
DpnI	GATC	8 cut(s)	140, 291, 609, 765, 1416, 1583, 1806, 1824
DpnII	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
DraI	TTTAAA	1 cut(s)	1170
DraIII	CACNNNGTG	1 cut(s)	1766
DrdI	GACNNNNNNGTC	1 cut(s)	694
DriI	GACNNNNNGTC	2 cut(s)	108, 965
DseDI	GACNNNNNNGTC	1 cut(s)	694
EaeI	YGGCCR	1 cut(s)	1045
Eam1104I	CTCTTC	2 cut(s)	411, 1899
Eam1105I	GACNNNNNGTC	2 cut(s)	108, 965
EarI	CTCTTC	2 cut(s)	411, 1899
Ecl136II	GAGCTC	1 cut(s)	255
Eco130I	CCWWGG	2 cut(s)	893, 1211
Eco24I	GRGCYC	1 cut(s)	257
Eco31I	GGTCTC	3 cut(s)	21, 29, 1885
Eco47I	GGWCC	4 cut(s)	790, 1347, 1435, 2281
Eco47III	AGCGCT	1 cut(s)	2167
Eco53kI	GAGCTC	1 cut(s)	255
Eco57I	CTGAAG	3 cut(s)	147, 281, 564
EcoICRI	GAGCTC	1 cut(s)	255
EcoNI	CCTNNNNNAGG	1 cut(s)	1461
EcoO109I	RGGNCCY	1 cut(s)	848
EcoRII	CCWGG	6 cut(s)	1047, 1377, 1420, 1431, 1695, 1899
EcoT14I	CCWWGG	2 cut(s)	893, 1211
EcoT22I	ATGCAT	1 cut(s)	2194
EcoT38I	GRGCYC	1 cut(s)	257
ErhI	CCWWGG	2 cut(s)	893, 1211
Esp3I	CGTCTC	2 cut(s)	609, 2245
FaqI	GGGAC	6 cut(s)	1193, 1205, 1760, 2083, 2157, 2294
FauI	CCCGC	3 cut(s)	838, 1533, 1656
FokI	GGATG	8 cut(s)	1337, 1784, 1861, 1874, 1965, 2103, 2163, 2272
FriOI	GRGCYC	1 cut(s)	257
FspBI	CTAG	2 cut(s)	650, 1871
GlaI	GCGC	3 cut(s)	261, 889, 2167
GsaI	CCCAGC	3 cut(s)	930, 1754, 2239
GsuI	CTGGAG	1 cut(s)	1718
HaeII	RGCGCY	1 cut(s)	2169
HaeIII	GGCC	4 cut(s)	302, 850, 1047, 1390
HapII	CCGG	3 cut(s)	82, 284, 1295
HgaI	GACGC	1 cut(s)	626
HhaI	GCGC	3 cut(s)	262, 890, 2168
Hin1I	GRCGYC	1 cut(s)	1206
Hin6I	GCGC	3 cut(s)	260, 888, 2166
HinP1I	GCGC	3 cut(s)	260, 888, 2166
HincII	GTYRAC	2 cut(s)	738, 1204
HindII	GTYRAC	2 cut(s)	738, 1204
HindIII	AAGCTT	1 cut(s)	423
HinfI	GANTC	9 cut(s)	90, 106, 214, 446, 653, 731, 1109, 2138, 2244
HpaII	CCGG	3 cut(s)	82, 284, 1295
HphI	GGTGA	9 cut(s)	278, 410, 494, 1004, 1256, 1367, 1478, 1799, 2189
Hpy166II	GTNNAC	6 cut(s)	738, 959, 1204, 1757, 2039, 2099
Hpy188I	TCNGA	8 cut(s)	89, 166, 700, 768, 1026, 1610, 1675, 2295
Hpy8I	GTNNAC	6 cut(s)	738, 959, 1204, 1757, 2039, 2099
HpyCH4III	ACNGT	7 cut(s)	493, 565, 1511, 1522, 1555, 2074, 2113
HpyCH4IV	ACGT	2 cut(s)	825, 1206
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1265
HpyF3I	CTNAG	8 cut(s)	86, 578, 1722, 1913, 2102, 2169, 2273, 2292
HpySE526I	ACGT	2 cut(s)	825, 1206
Hsp92I	GRCGYC	1 cut(s)	1206
HspAI	GCGC	3 cut(s)	260, 888, 2166
KroI	GCCGGC	1 cut(s)	81
KroNI	GCCGGC	1 cut(s)	83
Kzo9I	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
LmnI	GCTCC	6 cut(s)	125, 260, 754, 895, 1543, 1747
Lsp1109I	GCAGC	7 cut(s)	522, 815, 1208, 1499, 1663, 2275, 2305
LweI	GCATC	4 cut(s)	1648, 1899, 2125, 2253
MaeI	CTAG	2 cut(s)	650, 1871
MaeII	ACGT	2 cut(s)	825, 1206
MaeIII	GTNAC	3 cut(s)	1124, 1516, 1679
MalI	GATC	8 cut(s)	140, 291, 609, 765, 1416, 1583, 1806, 1824
MbiI	CCGCTC	1 cut(s)	525
MboI	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
MflI	RGATCY	1 cut(s)	289
MhlI	GDGCHC	3 cut(s)	206, 257, 1473
MlsI	TGGCCA	1 cut(s)	1047
MluCI	AATT	8 cut(s)	374, 504, 1445, 1604, 1626, 1782, 1839, 2296
MluNI	TGGCCA	1 cut(s)	1047
MlyI	GAGTC	3 cut(s)	662, 740, 2147
Mox20I	TGGCCA	1 cut(s)	1047
Mph1103I	ATGCAT	1 cut(s)	2194
MroNI	GCCGGC	1 cut(s)	81
MroXI	GAANNNNTTC	1 cut(s)	348
MscI	TGGCCA	1 cut(s)	1047
MseI	TTAA	8 cut(s)	75, 228, 630, 1169, 1331, 1548, 1620, 1707
MslI	CAYNNNNRTG	1 cut(s)	974
Msp20I	TGGCCA	1 cut(s)	1047
MspA1I	CMGCKG	2 cut(s)	806, 1490
MspCI	CTTAAG	1 cut(s)	1706
MspI	CCGG	3 cut(s)	82, 284, 1295
MspR9I	CCNGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
Mva1269I	GAATGC	4 cut(s)	463, 535, 890, 1919
MvaI	CCWGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
MvnI	CGCG	2 cut(s)	260, 1540
MwoI	GCNNNNNNNGC	1 cut(s)	1265
NaeI	GCCGGC	1 cut(s)	83
NcoI	CCATGG	1 cut(s)	893
NdeII	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
NgoMIV	GCCGGC	1 cut(s)	81
NlaIV	GGNNCC	7 cut(s)	291, 849, 981, 1005, 1389, 1646, 2282
NmeAIII	GCCGAG	1 cut(s)	931
NmuCI	GTSAC	1 cut(s)	1124
NsiI	ATGCAT	1 cut(s)	2194
NspI	RCATGY	1 cut(s)	668
NspV	TTCGAA	3 cut(s)	455, 558, 1923
PagI	TCATGA	1 cut(s)	858
PaqCI	CACCTGC	1 cut(s)	143
PctI	GAATGC	4 cut(s)	463, 535, 890, 1919
PdiI	GCCGGC	1 cut(s)	83
PdmI	GAANNNNTTC	1 cut(s)	348
PfeI	GAWTC	6 cut(s)	90, 106, 214, 446, 1109, 2244
Pfl23II	CGTACG	1 cut(s)	2130
PflFI	GACNNNGTC	1 cut(s)	1943
PflMI	CCANNNNNTGG	2 cut(s)	1054, 1784
PfoI	TCCNGGA	1 cut(s)	1420
PleI	GAGTC	3 cut(s)	661, 739, 2146
PpsI	GAGTC	3 cut(s)	661, 739, 2146
PshBI	ATTAAT	1 cut(s)	75
Psp124BI	GAGCTC	1 cut(s)	257
Psp6I	CCWGG	6 cut(s)	1047, 1377, 1420, 1431, 1695, 1899
PspFI	CCCAGC	3 cut(s)	926, 1750, 2235
PspGI	CCWGG	6 cut(s)	1047, 1377, 1420, 1431, 1695, 1899
PspLI	CGTACG	1 cut(s)	2130
PspN4I	GGNNCC	7 cut(s)	291, 849, 981, 1005, 1389, 1646, 2282
PspPI	GGNCC	6 cut(s)	790, 848, 1347, 1388, 1435, 2281
PsrI	GAACNNNNNNTAC	4 cut(s)	664, 696, 2036, 2068
PstI	CTGCAG	1 cut(s)	154
PstNI	CAGNNNCTG	2 cut(s)	170, 1397
PsuI	RGATCY	1 cut(s)	289
PsyI	GACNNNGTC	1 cut(s)	1943
PvuII	CAGCTG	2 cut(s)	806, 1490
RsaI	GTAC	5 cut(s)	673, 1971, 2132, 2225, 2327
RsaNI	GTAC	5 cut(s)	672, 1970, 2131, 2224, 2326
RseI	CAYNNNNRTG	1 cut(s)	974
SacI	GAGCTC	1 cut(s)	257
SaqAI	TTAA	8 cut(s)	75, 228, 630, 1169, 1331, 1548, 1620, 1707
Sau3AI	GATC	8 cut(s)	138, 289, 607, 763, 1414, 1581, 1804, 1822
Sau96I	GGNCC	6 cut(s)	790, 848, 1347, 1388, 1435, 2281
SchI	GAGTC	3 cut(s)	662, 740, 2147
ScrFI	CCNGG	6 cut(s)	1049, 1379, 1422, 1433, 1697, 1901
SduI	GDGCHC	3 cut(s)	206, 257, 1473
SfaNI	GCATC	4 cut(s)	1648, 1899, 2125, 2253
SfcI	CTRYAG	2 cut(s)	150, 879
SfuI	TTCGAA	3 cut(s)	455, 558, 1923
SinI	GGWCC	4 cut(s)	790, 1347, 1435, 2281
SmiMI	CAYNNNNRTG	1 cut(s)	974
SmlI	CTYRAG	3 cut(s)	205, 728, 1706
SmoI	CTYRAG	3 cut(s)	205, 728, 1706
SpeI	ACTAGT	1 cut(s)	1870
Sse9I	AATT	8 cut(s)	374, 504, 1445, 1604, 1626, 1782, 1839, 2296
SspMI	CTAG	2 cut(s)	650, 1871
SstI	GAGCTC	1 cut(s)	257
StyD4I	CCNGG	6 cut(s)	1047, 1377, 1420, 1431, 1695, 1899
StyI	CCWWGG	2 cut(s)	893, 1211
TaaI	ACNGT	7 cut(s)	493, 565, 1511, 1522, 1555, 2074, 2113
TaiI	ACGT	2 cut(s)	828, 1209
TaqI	TCGA	8 cut(s)	455, 521, 558, 899, 1318, 1821, 1923, 2218
TasI	AATT	8 cut(s)	374, 504, 1445, 1604, 1626, 1782, 1839, 2296
TatI	WGTACW	2 cut(s)	1969, 2223
TauI	GCSGC	5 cut(s)	235, 279, 528, 835, 1736
TfiI	GAWTC	6 cut(s)	90, 106, 214, 446, 1109, 2244
Tru1I	TTAA	8 cut(s)	75, 228, 630, 1169, 1331, 1548, 1620, 1707
Tru9I	TTAA	8 cut(s)	75, 228, 630, 1169, 1331, 1548, 1620, 1707
TscAI	CASTG	2 cut(s)	823, 1730
TseFI	GTSAC	1 cut(s)	1124
TseI	GCWGC	7 cut(s)	510, 803, 1196, 1487, 1651, 2288, 2318
Tsp45I	GTSAC	1 cut(s)	1124
TspDTI	ATGAA	9 cut(s)	53, 227, 609, 875, 1093, 1252, 1780, 2075, 2075
TspGWI	ACGGA	5 cut(s)	689, 1112, 1754, 1833, 2118
TspRI	CASTG	2 cut(s)	823, 1730
Tth111I	GACNNNGTC	1 cut(s)	1943
Van91I	CCANNNNNTGG	2 cut(s)	1054, 1784
Vha464I	CTTAAG	1 cut(s)	1706
VpaK11BI	GGWCC	4 cut(s)	790, 1347, 1435, 2281
VspI	ATTAAT	1 cut(s)	75
XagI	CCTNNNNNAGG	1 cut(s)	1461
XapI	RAATTY	1 cut(s)	1604
XceI	RCATGY	1 cut(s)	668
XmnI	GAANNNNTTC	1 cut(s)	348
XspI	CTAG	2 cut(s)	650, 1871
ZraI	GACGTC	1 cut(s)	1207
Zsp2I	ATGCAT	1 cut(s)	2194

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.