Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012444

Physical Location & Seq

Genomic Coordinates Forward (+)

3471278 .. 3475714

Gene Structure 4437 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1052000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGGCTTCCATTGGGTCTCTGCATTTTATCATGGGGACCAAGACTGATAGCTCTGTGCTCGTTCACAGCAGTACTAATCCCCGGCTCAGATTCCCATTCTTCACGAATCGGCCAAGAAGCTCCATTGAAGTTAGTGGGATCAATGCTTCTGCAAATGGGTCTGCAGGAGCTGCGCGAATGTGCAGAGGACGGAATAGTTGGTGCACAAGAGGGAAACTTTGGAGGATTAAGCCGCTGAGAGCGATCCGAAAGAGCTCGGCGGAAGGTGAGGACGCGGGCGAGCCGGAGGACGCGCTTCAGGCCACCATTGAAAAGAGCAAGAAGGTTCTTGCTGTGCAAAGAGAACTACTACAACAGATTTCTGAAAGAAGGAAATTGGTATCTTCTATACAAAATAGTGATATAAGCCAAGAAGAAGGGGAAGCTTCTTTTGAACAAGGGAATGAATCGTTTTCGAATGCGGAAAGTTCTTCAAGTAGTGTTGATAAAAGTGTTGACCAAATTGGCAGCATTGTTTTGAGCAGCTATGAGCATTCAACTGAAGAAAAGGATTTTGAAACCGTGCCTTCTGCTCTTAGTGGAGGGCTTGATGAAATAGAAAAGGATCATGGAGATGCTTTACCATTTGATAAGTCTCCCAGTGAACTAGAGTCCAGTAACCATGTGGGTAAGGATAGTTCCAAGACAGAGTGGTCTGATGGTTTGCCAACTTTTCTTACTAACCCTGAGTCGTCTATGCTAAAAGATGACGAGCTTATTGATTTGAAAGAAACAAGTGAAGAGGAGGTCAGTGATGGGGCAACTGGTTTTACGAGTGAGGACGTAAAGCCCCCTCCTTTGGCTGGGCCTAATGTCATGAATGTCATATTGGTAGCTGCAGAATGTGCTCCATGGTCGAAAACAGGTGGGCTTGGAGATGTTGCTGGGGCTTTACCAAAGGCTTTGGCTCGGCGTGGACACAGGGTCATGGTTGTGGCACCTCGCTACGGTGATTATGCGGAACCCCAAGATTCAGGAGTTCGGAAAGCATATAAAGTTGATGGCCAGTATATGGAAGTAGCATATTTCCATGCCTATATTGATGGTGTAGATTTTGTCTTCATTGAATCTGCTGTGTTCCGTCACATGGAGCACAATATATATGGAGGAAAGCGTGAGGATATTTTAAAACGCATGGTGTTATTTTGCAAGGCAGCAGTTGAGGTCCCTTGGCTTGTCCCCTGTGGCGGTGTTTGCTATGGTGATGGAAACTTGGCTTTCATTGCAAATGATTGGCATACTGCATTGTTGCCGGTGTATTTGAAGGCATATTATCGGGACAATGGTTTAATGATATATACAAGATCCATCCTTGTAATCCATAACATAGCTCACCAGGGTCGGGGGCCAGTAGCTGATTTCTCCTTCGTTGATCTTCCGGGACACTACCTGGACCTTTTCAAAATGTATGACCCTATTGGAGGCGAGCACTTCAATATCTTTGCAGCTGGTCTAAAGACAGCAGACCGTGTGGTTACTGTGAGTCATGGATATGCGTGGGAGCTTAAAACGGTTGACGGTGGTTGGGGATTGCACGGGATCATAAATGAGAATGACTGGAAATTCAGAGGCATTGTTAATGGAATTGACACAGAAGACTGGAATCCACAGTATGATGTTTTCTTGAAATCAGATGGTTACACGAACTACTCCCTGGAAACACTTAAGACTGGCAAGGCTCAGTGTAAGGCAGCCTTACAGAAGGAACTGGGTTTACCAGTCCGTGAGGATGTCCCCGTAATTGGTTTCATTGGGAGGCTGGATCACCAGAAGGGTGTTGATCTCATAGCAGAGGCAATTCCTTGGATGATGGGTCAGGATGTGCAACTAATCATGTTAGGCTCTGGCAGACCTGACCTGGAAGAGATGCTAAGGGCATTTGAACATCAACACCGAGACAAGGTCAGGGGATGGGTTGGGTTTTCTGTACAAACGGCTCACAGGATAACTGCCGGTGCAGACATTTTGCTCATGCCATCAAGATTTGAGCCGTGTGGATTGAACCAACTATACGCTATGAACTATGGGACAATTCCAGTTGTTCATGCTGTTGGTGGACTAAGAGATACCGTGCATCCTTTCAATCCGTACGAGGAGTCAGGACTTGGGTGGACATTTGACAGCGCTGAGGTCGGTAAGCTAATCCATGCATTAGGGAACTGCTTCTATACGTATCGAGACTACAAGCAGAGCTGGGAAGGAATCCAGAGACGAGGGATGATGCAAGACCTGAGCTGGGATCATGCTGCTCAGAACTATGAGGAGGTTCTTGTTGCTGCAAAGTACCAATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009011 GO:0009058 GO:0009059 GO:0009250 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

Glyco_trans_1_4 Glyco_transf_5 Glycos_transf_1

Protein Analysis

778

Amino Acids

86.13

Weight (kDa)

5.35

Isoelectric Point (pI)

39.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_5	PF08323	287 - 529	2.2e-57	Starch synthase catalytic domain
Glycos_transf_1	PF00534	587 - 707	4.9e-15	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	592 - 705	4.4e-16	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012444)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01180
fragaria_vesca	FvH4_6g48410 FvH4_6g48410 FvH4_6g48410
malus_domestica	MD00G1130900.v1.1 MD09G1052000.v1.1
prunus_persica	Prupe.3G266700_v2.0.a1 Prupe.3G266700_v2.0.a1
pyrus_communis	pycom111g04330
rosa_chinensis	RchiOBHm_Chr2g0168331
rosa_laevigata	RLG00000021767
rosa_multiflora	Rmu_sc0003354.1_g000016
rosa_roxburghii	Rroxscaffold_2G00083140
rosa_rugosa	Rorug02G0532700
rosa_samantha	Rh2AG602000 Rh2BG615300 Rh2CG583000 Rh2DG625500
rosa_wichuraiana	Rw2G049970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	691
AccB1I	GGYRCC	1 cut(s)	976
AccB7I	CCANNNNNTGG	1 cut(s)	1051
AccII	CGCG	3 cut(s)	175, 275, 293
AciI	CCGC	6 cut(s)	233, 260, 275, 461, 998, 1227
AclWI	GGATC	7 cut(s)	146, 238, 612, 1338, 1586, 1809, 2286
AcoI	YGGCCR	2 cut(s)	110, 1042
AcsI	RAATTY	1 cut(s)	1601
AcuI	CTGAAG	2 cut(s)	281, 561
AfaI	GTAC	4 cut(s)	73, 1968, 2129, 2324
AfeI	AGCGCT	1 cut(s)	2164
AflII	CTTAAG	1 cut(s)	1703
AhdI	GACNNNNNGTC	1 cut(s)	962
AjnI	CCWGG	4 cut(s)	1374, 1428, 1692, 1896
AjuI	GAANNNNNNNTTGG	2 cut(s)	851, 883
Alw21I	GWGCWC	6 cut(s)	60, 206, 257, 889, 1134, 1470
Alw26I	GTCTC	5 cut(s)	21, 639, 1929, 2211, 2242
Alw44I	GTGCAC	1 cut(s)	202
AlwI	GGATC	7 cut(s)	146, 238, 612, 1338, 1586, 1809, 2286
AlwNI	CAGNNNCTG	2 cut(s)	170, 1394
Aor51HI	AGCGCT	1 cut(s)	2164
AoxI	GGCC	5 cut(s)	110, 300, 845, 1042, 1385
ApaLI	GTGCAC	1 cut(s)	202
ApeKI	GCWGC	9 cut(s)	170, 507, 522, 875, 1193, 1484, 1730, 2285, 2315
ApoI	RAATTY	1 cut(s)	1601
Asp700I	GAANNNNTTC	1 cut(s)	2201
AspLEI	GCGC	3 cut(s)	175, 295, 2165
AspS9I	GGNCC	5 cut(s)	36, 845, 1204, 1385, 1432
AsuC2I	CCSGG	2 cut(s)	82, 1419
AsuHPI	GGTGA	5 cut(s)	278, 1001, 1253, 1364, 1796
AsuII	TTCGAA	1 cut(s)	455
AvaII	GGWCC	3 cut(s)	36, 1204, 1432
BaeGI	GKGCMC	1 cut(s)	206
BalI	TGGCCA	1 cut(s)	1044
BanI	GGYRCC	1 cut(s)	976
BanII	GRGCYC	1 cut(s)	257
BbsI	GAAGAC	2 cut(s)	1090, 1641
Bbv12I	GWGCWC	6 cut(s)	60, 206, 257, 889, 1134, 1470
BbvCI	CCTCAGC	1 cut(s)	2166
BbvI	GCAGC	9 cut(s)	157, 519, 534, 862, 1205, 1496, 1742, 2272, 2302
BceAI	ACGGC	2 cut(s)	1989, 2014
BciT130I	CCWGG	4 cut(s)	1376, 1430, 1694, 1898
BcnI	CCSGG	2 cut(s)	82, 1419
BcoDI	GTCTC	5 cut(s)	21, 639, 1929, 2211, 2242
BfaI	CTAG	1 cut(s)	647
BfmI	CTRYAG	2 cut(s)	162, 876
BfoI	RGCGCY	1 cut(s)	2166
BfrI	CTTAAG	1 cut(s)	1703
BmcAI	AGTACT	1 cut(s)	73
Bme1390I	CCNGG	6 cut(s)	82, 1376, 1419, 1430, 1694, 1898
Bme18I	GGWCC	3 cut(s)	36, 1204, 1432
BmeRI	GACNNNNNGTC	1 cut(s)	962
BmgT120I	GGNCC	5 cut(s)	36, 845, 1204, 1385, 1432
BmiI	GGNNCC	5 cut(s)	37, 978, 1002, 1206, 1386
BmrFI	CCNGG	6 cut(s)	82, 1376, 1419, 1430, 1694, 1898
BmrI	ACTGGG	2 cut(s)	633, 1757
BmsI	GCATC	4 cut(s)	604, 1896, 2122, 2250
BmuI	ACTGGG	2 cut(s)	633, 1757
BpiI	GAAGAC	2 cut(s)	1090, 1641
Bpu10I	CCTNAGC	3 cut(s)	1910, 2166, 2270
Bpu14I	TTCGAA	1 cut(s)	455
BpuMI	CCSGG	2 cut(s)	82, 1419
BsaAI	YACGTR	1 cut(s)	2211
BsaI	GGTCTC	1 cut(s)	21
BsaJI	CCNNGG	6 cut(s)	80, 890, 1208, 1375, 1692, 1841
Bse118I	RCCGGY	2 cut(s)	1291, 1991
Bse3DI	GCAATG	1 cut(s)	1260
BseBI	CCWGG	4 cut(s)	1376, 1430, 1694, 1898
BseDI	CCNNGG	6 cut(s)	80, 890, 1208, 1375, 1692, 1841
BseGI	GGATG	7 cut(s)	1347, 1774, 1851, 1864, 1955, 2113, 2262
BseMI	GCAATG	1 cut(s)	1260
BseMII	CTCAG	7 cut(s)	100, 227, 717, 1733, 2157, 2261, 2303
BseRI	GAGGAG	3 cut(s)	797, 2147, 2315
BseSI	GKGCMC	1 cut(s)	206
BseXI	GCAGC	9 cut(s)	157, 519, 534, 862, 1205, 1496, 1742, 2272, 2302
BseYI	CCCAGC	4 cut(s)	842, 923, 2232, 2274
BsgI	GTGCAG	2 cut(s)	202, 2016
Bsh1236I	CGCG	3 cut(s)	175, 275, 293
BshFI	GGCC	5 cut(s)	112, 302, 847, 1044, 1387
BshNI	GGYRCC	1 cut(s)	976
BsiHKAI	GWGCWC	6 cut(s)	60, 206, 257, 889, 1134, 1470
BsiSI	CCGG	5 cut(s)	82, 284, 1292, 1418, 1992
BsiWI	CGTACG	1 cut(s)	2127
BslFI	GGGAC	7 cut(s)	49, 1190, 1202, 1331, 1434, 1757, 2080
BsmAI	GTCTC	5 cut(s)	21, 639, 1929, 2211, 2242
BsmBI	CGTCTC	1 cut(s)	2242
BsmFI	GGGAC	7 cut(s)	49, 1190, 1202, 1331, 1434, 1757, 2080
BsmI	GAATGC	2 cut(s)	463, 532
BsnI	GGCC	5 cut(s)	112, 302, 847, 1044, 1387
Bso31I	GGTCTC	1 cut(s)	21
Bsp119I	TTCGAA	1 cut(s)	455
Bsp1286I	GDGCHC	6 cut(s)	60, 206, 257, 889, 1134, 1470
Bsp1407I	TGTACA	1 cut(s)	1966
Bsp143I	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
Bsp19I	CCATGG	1 cut(s)	890
BspACI	CCGC	6 cut(s)	233, 260, 275, 461, 998, 1227
BspANI	GGCC	5 cut(s)	112, 302, 847, 1044, 1387
BspCNI	CTCAG	7 cut(s)	99, 228, 718, 1732, 2158, 2262, 2302
BspFNI	CGCG	3 cut(s)	175, 275, 293
BspHI	TCATGA	1 cut(s)	855
BspLI	GGNNCC	5 cut(s)	37, 978, 1002, 1206, 1386
BspMAI	CTGCAG	2 cut(s)	166, 880
BspPI	GGATC	7 cut(s)	146, 238, 612, 1338, 1586, 1809, 2286
BspT104I	TTCGAA	1 cut(s)	455
BspT107I	GGYRCC	1 cut(s)	976
BspTI	CTTAAG	1 cut(s)	1703
BspTNI	GGTCTC	1 cut(s)	21
BsrDI	GCAATG	1 cut(s)	1260
BsrFI	RCCGGY	2 cut(s)	1291, 1991
BsrGI	TGTACA	1 cut(s)	1966
BssAI	RCCGGY	2 cut(s)	1291, 1991
BssECI	CCNNGG	6 cut(s)	80, 890, 1208, 1375, 1692, 1841
BssMI	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
BssT1I	CCWWGG	3 cut(s)	890, 1208, 1841
Bst2UI	CCWGG	4 cut(s)	1376, 1430, 1694, 1898
Bst4CI	ACNGT	8 cut(s)	562, 989, 1508, 1519, 1552, 1559, 1650, 2110
Bst6I	CTCTTC	2 cut(s)	774, 1896
BstAFI	CTTAAG	1 cut(s)	1703
BstAPI	GCANNNNNTGC	2 cut(s)	170, 884
BstAUI	TGTACA	1 cut(s)	1966
BstBAI	YACGTR	1 cut(s)	2211
BstBI	TTCGAA	1 cut(s)	455
BstC8I	GCNNGC	3 cut(s)	277, 281, 1466
BstDSI	CCRYGG	1 cut(s)	890
BstENI	CCTNNNNNAGG	1 cut(s)	1458
BstF5I	GGATG	7 cut(s)	1347, 1774, 1851, 1864, 1955, 2113, 2262
BstFNI	CGCG	3 cut(s)	175, 275, 293
BstH2I	RGCGCY	1 cut(s)	2166
BstHHI	GCGC	3 cut(s)	175, 295, 2165
BstKTI	GATC	9 cut(s)	141, 246, 607, 1346, 1414, 1581, 1804, 1822, 2281
BstMAI	GTCTC	5 cut(s)	21, 639, 1929, 2211, 2242
BstMBI	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
BstMWI	GCNNNNNNNGC	5 cut(s)	170, 299, 884, 1233, 1262
BstNI	CCWGG	4 cut(s)	1376, 1430, 1694, 1898
BstSCI	CCNGG	6 cut(s)	80, 1374, 1417, 1428, 1692, 1896
BstSFI	CTRYAG	2 cut(s)	162, 876
BstSLI	GKGCMC	1 cut(s)	206
BstSNI	TACGTA	1 cut(s)	2211
BstUI	CGCG	3 cut(s)	175, 275, 293
BstV1I	GCAGC	9 cut(s)	157, 519, 534, 862, 1205, 1496, 1742, 2272, 2302
BstV2I	GAAGAC	2 cut(s)	1090, 1641
BstX2I	RGATCY	1 cut(s)	1343
BstYI	RGATCY	1 cut(s)	1343
BsuRI	GGCC	5 cut(s)	112, 302, 847, 1044, 1387
BtgI	CCRYGG	1 cut(s)	890
BtsCI	GGATG	7 cut(s)	1347, 1774, 1851, 1864, 1955, 2113, 2262
BtsIMutI	CAGTG	3 cut(s)	646, 796, 1727
Cac8I	GCNNGC	3 cut(s)	277, 281, 1466
CaiI	CAGNNNCTG	2 cut(s)	170, 1394
CciI	TCATGA	1 cut(s)	855
CfoI	GCGC	3 cut(s)	175, 295, 2165
Cfr10I	RCCGGY	2 cut(s)	1291, 1991
Cfr13I	GGNCC	5 cut(s)	36, 845, 1204, 1385, 1432
CseI	GACGC	2 cut(s)	281, 299
Csp6I	GTAC	4 cut(s)	72, 1967, 2128, 2323
CviQI	GTAC	4 cut(s)	72, 1967, 2128, 2323
DpnI	GATC	9 cut(s)	140, 245, 606, 1345, 1413, 1580, 1803, 1821, 2280
DpnII	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
DraI	TTTAAA	1 cut(s)	1167
DrdI	GACNNNNNNGTC	1 cut(s)	691
DriI	GACNNNNNGTC	1 cut(s)	962
DseDI	GACNNNNNNGTC	1 cut(s)	691
EaeI	YGGCCR	2 cut(s)	110, 1042
Eam1104I	CTCTTC	2 cut(s)	774, 1896
Eam1105I	GACNNNNNGTC	1 cut(s)	962
EarI	CTCTTC	2 cut(s)	774, 1896
EciI	GGCGGA	1 cut(s)	275
Ecl136II	GAGCTC	1 cut(s)	255
Eco105I	TACGTA	1 cut(s)	2211
Eco130I	CCWWGG	3 cut(s)	890, 1208, 1841
Eco24I	GRGCYC	1 cut(s)	257
Eco31I	GGTCTC	1 cut(s)	21
Eco47I	GGWCC	3 cut(s)	36, 1204, 1432
Eco47III	AGCGCT	1 cut(s)	2164
Eco53kI	GAGCTC	1 cut(s)	255
Eco57I	CTGAAG	2 cut(s)	281, 561
EcoICRI	GAGCTC	1 cut(s)	255
EcoNI	CCTNNNNNAGG	1 cut(s)	1458
EcoO109I	RGGNCCY	1 cut(s)	1204
EcoRII	CCWGG	4 cut(s)	1374, 1428, 1692, 1896
EcoT14I	CCWWGG	3 cut(s)	890, 1208, 1841
EcoT22I	ATGCAT	1 cut(s)	2191
EcoT38I	GRGCYC	1 cut(s)	257
ErhI	CCWWGG	3 cut(s)	890, 1208, 1841
Esp3I	CGTCTC	1 cut(s)	2242
FalI	AAGNNNNNCTT	2 cut(s)	1718, 1750
FaqI	GGGAC	7 cut(s)	49, 1190, 1202, 1331, 1434, 1757, 2080
FauI	CCCGC	1 cut(s)	268
FokI	GGATG	7 cut(s)	1334, 1781, 1858, 1871, 1962, 2100, 2269
FriOI	GRGCYC	1 cut(s)	257
FspBI	CTAG	1 cut(s)	647
GlaI	GCGC	3 cut(s)	174, 294, 2164
GsaI	CCCAGC	4 cut(s)	846, 927, 2236, 2278
HaeII	RGCGCY	1 cut(s)	2166
HaeIII	GGCC	5 cut(s)	112, 302, 847, 1044, 1387
HapII	CCGG	5 cut(s)	82, 284, 1292, 1418, 1992
HgaI	GACGC	2 cut(s)	281, 299
HhaI	GCGC	3 cut(s)	175, 295, 2165
Hin6I	GCGC	3 cut(s)	173, 293, 2163
HinP1I	GCGC	3 cut(s)	173, 293, 2163
HincII	GTYRAC	2 cut(s)	496, 1555
HindII	GTYRAC	2 cut(s)	496, 1555
HindIII	AAGCTT	1 cut(s)	423
HpaII	CCGG	5 cut(s)	82, 284, 1292, 1418, 1992
HphI	GGTGA	5 cut(s)	278, 1001, 1253, 1364, 1796
Hpy166II	GTNNAC	9 cut(s)	64, 204, 496, 644, 956, 1555, 1754, 2096, 2151
Hpy188I	TCNGA	8 cut(s)	89, 248, 364, 697, 1023, 1607, 1672, 2292
Hpy8I	GTNNAC	9 cut(s)	64, 204, 496, 644, 956, 1555, 1754, 2096, 2151
HpyCH4III	ACNGT	8 cut(s)	562, 989, 1508, 1519, 1552, 1559, 1650, 2110
HpyCH4IV	ACGT	2 cut(s)	822, 2210
HpyF10VI	GCNNNNNNNGC	5 cut(s)	170, 299, 884, 1233, 1262
HpySE526I	ACGT	2 cut(s)	822, 2210
HspAI	GCGC	3 cut(s)	173, 293, 2163
Kzo9I	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
LmnI	GCTCC	5 cut(s)	125, 167, 892, 1129, 1540
Lsp1109I	GCAGC	9 cut(s)	157, 519, 534, 862, 1205, 1496, 1742, 2272, 2302
LweI	GCATC	4 cut(s)	604, 1896, 2122, 2250
MaeI	CTAG	1 cut(s)	647
MaeII	ACGT	2 cut(s)	822, 2210
MaeIII	GTNAC	4 cut(s)	656, 1121, 1513, 1676
MalI	GATC	9 cut(s)	140, 245, 606, 1345, 1413, 1580, 1803, 1821, 2280
MboI	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
MflI	RGATCY	1 cut(s)	1343
MhlI	GDGCHC	6 cut(s)	60, 206, 257, 889, 1134, 1470
MlsI	TGGCCA	1 cut(s)	1044
MluCI	AATT	7 cut(s)	374, 501, 1601, 1623, 1779, 1836, 2070
MluNI	TGGCCA	1 cut(s)	1044
MlyI	GAGTC	4 cut(s)	659, 737, 1531, 2144
Mox20I	TGGCCA	1 cut(s)	1044
Mph1103I	ATGCAT	1 cut(s)	2191
MroXI	GAANNNNTTC	1 cut(s)	2201
MscI	TGGCCA	1 cut(s)	1044
MseI	TTAA	6 cut(s)	228, 1166, 1328, 1545, 1617, 1704
MslI	CAYNNNNRTG	3 cut(s)	612, 971, 1530
Msp20I	TGGCCA	1 cut(s)	1044
MspA1I	CMGCKG	2 cut(s)	235, 1487
MspCI	CTTAAG	1 cut(s)	1703
MspI	CCGG	5 cut(s)	82, 284, 1292, 1418, 1992
MspR9I	CCNGG	6 cut(s)	82, 1376, 1419, 1430, 1694, 1898
Mva1269I	GAATGC	2 cut(s)	463, 532
MvaI	CCWGG	4 cut(s)	1376, 1430, 1694, 1898
MvnI	CGCG	3 cut(s)	175, 275, 293
MwoI	GCNNNNNNNGC	5 cut(s)	170, 299, 884, 1233, 1262
NciI	CCSGG	2 cut(s)	82, 1419
NcoI	CCATGG	1 cut(s)	890
NdeII	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
NlaIV	GGNNCC	5 cut(s)	37, 978, 1002, 1206, 1386
NmeAIII	GCCGAG	2 cut(s)	236, 928
NmuCI	GTSAC	1 cut(s)	1121
NsiI	ATGCAT	1 cut(s)	2191
NspV	TTCGAA	1 cut(s)	455
PagI	TCATGA	1 cut(s)	855
PctI	GAATGC	2 cut(s)	463, 532
PdmI	GAANNNNTTC	1 cut(s)	2201
PfeI	GAWTC	7 cut(s)	90, 106, 446, 1010, 1106, 1642, 2241
Pfl23II	CGTACG	1 cut(s)	2127
PflFI	GACNNNGTC	1 cut(s)	1940
PflMI	CCANNNNNTGG	1 cut(s)	1051
PfoI	TCCNGGA	1 cut(s)	1417
PleI	GAGTC	4 cut(s)	658, 736, 1530, 2143
PpsI	GAGTC	4 cut(s)	658, 736, 1530, 2143
Ppu21I	YACGTR	1 cut(s)	2211
PpuMI	RGGWCCY	1 cut(s)	1204
Psp124BI	GAGCTC	1 cut(s)	257
Psp5II	RGGWCCY	1 cut(s)	1204
Psp6I	CCWGG	4 cut(s)	1374, 1428, 1692, 1896
PspFI	CCCAGC	4 cut(s)	842, 923, 2232, 2274
PspGI	CCWGG	4 cut(s)	1374, 1428, 1692, 1896
PspLI	CGTACG	1 cut(s)	2127
PspN4I	GGNNCC	5 cut(s)	37, 978, 1002, 1206, 1386
PspPI	GGNCC	5 cut(s)	36, 845, 1204, 1385, 1432
PspPPI	RGGWCCY	1 cut(s)	1204
PsrI	GAACNNNNNNTAC	6 cut(s)	661, 693, 1737, 1769, 2033, 2065
PstI	CTGCAG	2 cut(s)	166, 880
PstNI	CAGNNNCTG	2 cut(s)	170, 1394
PsuI	RGATCY	1 cut(s)	1343
PsyI	GACNNNGTC	1 cut(s)	1940
PvuII	CAGCTG	1 cut(s)	1487
RsaI	GTAC	4 cut(s)	73, 1968, 2129, 2324
RsaNI	GTAC	4 cut(s)	72, 1967, 2128, 2323
RseI	CAYNNNNRTG	3 cut(s)	612, 971, 1530
SacI	GAGCTC	1 cut(s)	257
SaqAI	TTAA	6 cut(s)	228, 1166, 1328, 1545, 1617, 1704
Sau3AI	GATC	9 cut(s)	138, 243, 604, 1343, 1411, 1578, 1801, 1819, 2278
Sau96I	GGNCC	5 cut(s)	36, 845, 1204, 1385, 1432
ScaI	AGTACT	1 cut(s)	73
SchI	GAGTC	4 cut(s)	659, 737, 1531, 2144
ScrFI	CCNGG	6 cut(s)	82, 1376, 1419, 1430, 1694, 1898
SduI	GDGCHC	6 cut(s)	60, 206, 257, 889, 1134, 1470
SfaNI	GCATC	4 cut(s)	604, 1896, 2122, 2250
SfcI	CTRYAG	2 cut(s)	162, 876
SfuI	TTCGAA	1 cut(s)	455
SinI	GGWCC	3 cut(s)	36, 1204, 1432
SmiMI	CAYNNNNRTG	3 cut(s)	612, 971, 1530
SmlI	CTYRAG	1 cut(s)	1703
SmoI	CTYRAG	1 cut(s)	1703
SnaBI	TACGTA	1 cut(s)	2211
Sse9I	AATT	7 cut(s)	374, 501, 1601, 1623, 1779, 1836, 2070
SsiI	CCGC	6 cut(s)	233, 260, 275, 461, 998, 1227
SspMI	CTAG	1 cut(s)	647
SstI	GAGCTC	1 cut(s)	257
StyD4I	CCNGG	6 cut(s)	80, 1374, 1417, 1428, 1692, 1896
StyI	CCWWGG	3 cut(s)	890, 1208, 1841
TaaI	ACNGT	8 cut(s)	562, 989, 1508, 1519, 1552, 1559, 1650, 2110
TaiI	ACGT	2 cut(s)	825, 2213
TaqI	TCGA	3 cut(s)	455, 896, 2215
TasI	AATT	7 cut(s)	374, 501, 1601, 1623, 1779, 1836, 2070
TatI	WGTACW	2 cut(s)	71, 1966
TauI	GCSGC	1 cut(s)	235
TfiI	GAWTC	7 cut(s)	90, 106, 446, 1010, 1106, 1642, 2241
Tru1I	TTAA	6 cut(s)	228, 1166, 1328, 1545, 1617, 1704
Tru9I	TTAA	6 cut(s)	228, 1166, 1328, 1545, 1617, 1704
TscAI	CASTG	3 cut(s)	646, 796, 1727
TseFI	GTSAC	1 cut(s)	1121
TseI	GCWGC	9 cut(s)	170, 507, 522, 875, 1193, 1484, 1730, 2285, 2315
Tsp45I	GTSAC	1 cut(s)	1121
TspDTI	ATGAA	8 cut(s)	459, 606, 872, 1090, 1249, 1777, 2072, 2072
TspGWI	ACGGA	4 cut(s)	205, 1109, 1751, 2115
TspRI	CASTG	3 cut(s)	646, 796, 1727
Tth111I	GACNNNGTC	1 cut(s)	1940
Van91I	CCANNNNNTGG	1 cut(s)	1051
Vha464I	CTTAAG	1 cut(s)	1703
VneI	GTGCAC	1 cut(s)	202
VpaK11BI	GGWCC	3 cut(s)	36, 1204, 1432
XagI	CCTNNNNNAGG	1 cut(s)	1458
XapI	RAATTY	1 cut(s)	1601
XcmI	CCANNNNNNNNNTGG	1 cut(s)	661
XmnI	GAANNNNTTC	1 cut(s)	2201
XspI	CTAG	1 cut(s)	647
ZrmI	AGTACT	1 cut(s)	73
Zsp2I	ATGCAT	1 cut(s)	2191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.