Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0012444

Physical Location & Seq

Genomic Coordinates Forward (+)

5868971 .. 5874486

Gene Structure 5516 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00083140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGCAAAGAGACCTACTTCAACAAATTGCTGAAAGAAGGAAACTGGTATCTTCTATAACAAGTAGTACTGTAGAAGAAGAAGAAAATCAAGAAGAGGTTGATTCTTTTGAGCAAGAGGATTCTTTTTCAGATGTAGAAAGTTCTTCAGGTGGTGGTAATGACACTATTGAAGTACAAAATGACAGCATTGCTTCAGGCAGCTATGGTCATTCAACTGCAGAAAAGGAACTAGAAACAGTACCTTCTGCTGTTAGTAGAAGCTTTAGTGAAATTGAGAAGCATGAAGAAACTGTGCCTCTTAATAAGGCTCCCTATGAGACAGAGTCCACAAAGCGTGTGAATAAGACTAGTGCCAAGTCAGAGTGGTCCGATTCTCTGCCATCTTTTATTTCAACTACTGTTGAGACGTCCTCTCTGAAAGATGAAGAAGAGCATGTAGATCTGAAAGAACCAAGTTTACAGGAGGTCAATGTTGGGGCAATGGATCCTACAATCGAAGACGTGAAGCCCCCTCCTTTGGCTGGGCCCAATGTCATGAATATCATATTGGTGGCAGCAGAATGTGCTCCGTGGTCGAAAACAGGTGGGCTTGGAGATGTTGCTGGAGCTTTACCAAAAGCTTTGGCTCGGCGTGGCCATAGGGTTATGGTTGTGGCACCTCGGTATGGCAATTATGCTGAACCTCAAGATTTAGGAGTCCGGAAAGGATATAAAGTAGATGGCCAGCATATGGAAGTAGCATACTTTCACTCCTATATTGATGGCGTAGATTTTGTCTTCATTGACTCTCCTATGTTCCGTCACATGGAGAATAATATATACAGAGGAAAGCGTGAGGATATCTTAAAGCGGATGGTGCTGTTTTGCAAGGCGGCTGTTGAGGCCCCTTGGCATGTTCCCTGTGGTGGCATTTGCTATGGTGATGGAAACTTGGCTTTCATTGCAAATGACTGGCATACTGCACTGTTGCCAGTGTATTTGAAGGCGTACTATCGAGACAATGGCTTGATGATGTATACAAGGTCCATCCTTGTAATCCATAACATAGCTCACCAGGGTCGTGGGCCAGTAAGTGATTTCTCCTTCGTTGATCTTCCACAACACTACTTGGACCTTTTCAAATTGTATGATCCCGTTGGAGGGGAGCACTTCAATATCTTTGCAGCTGGTTTGAAGACAGCCGACCGTGTGGTTACTGTAAGTCATGGATATGCCTGGGAGGTTAAAACACCTGAAGGTGGTTGGGGATTGCATGGGATCATAAATGAAAATCACTGGAAACTCAGCGGCATTGTTAATGGAATTGATAATAAAGACTGGAATCCACAGTTTGATGTTTTCTTGAGATCAGATGGTTATAGAAACTACTCCCTAGAGACACTTAAGACTGGCAAGCCTCAGTGTAAGGCAGCTTTACAGAAGGAGCTGGGTTTGCCGATCCGTGAGGATGTCCCTTTAATTGGTTTCATTGGGAGACTGGATCCCCAGAAAGGTGTTGATCTTATAGCACAGGCGATTCCGTGGATGATGGAACAAGATGTGCAACTAGTCATGTTAGGCACTGGAAGACCTGACCTGGAACAGATGCTGAAAGAGTTTGAACATCAACACAGAGACAAGGTCAGGGGATGGGTTGGTTTTTCTGTAGAAACAGCTCATAGAATAACTGCCGGTGTAGACATTTTGCTCATGCCATCGAGATTTGAGCCTTGTGGACTGAACCAACTATATGCCATGAACTATGGGACAGTTCCGGTCGTACATGCTGTTGGTGGACTCAGAGATACTGTACATCCTTTCAATCCATATGAGGAGTCAGGACTTGGGTGGACATTTGACAGCGCTGATACAGGTAAGCTCATCCATGCATTAGGTAATTGCATATATACTTACCGAGAGTACAAGCAGAGTTGGGAAGGAATCCAGAGACGAGGGATGATGCAAGACCTAAGTTGGGACAATGCTGCTCAGAAGTATGAGGAGGTTGTTGTGGCTGCAAAGTACCAATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009011 GO:0009058 GO:0009059 GO:0009250 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

Glyco_trans_1_4 Glyco_transf_5 Glycos_transf_1

Protein Analysis

670

Amino Acids

75.1

Weight (kDa)

5.19

Isoelectric Point (pI)

43.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_5	PF08323	180 - 422	2.6e-61	Starch synthase catalytic domain
Glycos_transf_1	PF00534	479 - 600	1.2e-14	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	485 - 623	2e-16	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012444)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01180
fragaria_vesca	FvH4_6g48410 FvH4_6g48410 FvH4_6g48410
malus_domestica	MD00G1130900.v1.1 MD09G1052000.v1.1
prunus_persica	Prupe.3G266700_v2.0.a1 Prupe.3G266700_v2.0.a1
pyrus_communis	pycom111g04330
rosa_chinensis	RchiOBHm_Chr2g0168331
rosa_laevigata	RLG00000021767
rosa_multiflora	Rmu_sc0003354.1_g000016
rosa_roxburghii	Rroxscaffold_2G00083140
rosa_rugosa	Rorug02G0532700
rosa_samantha	Rh2AG602000 Rh2BG615300 Rh2CG583000 Rh2DG625500
rosa_wichuraiana	Rw2G049970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	410
AccB1I	GGYRCC	1 cut(s)	655
AccB7I	CCANNNNNTGG	1 cut(s)	730
AccI	GTMKAC	2 cut(s)	1016, 1677
AccIII	TCCGGA	1 cut(s)	699
AciI	CCGC	3 cut(s)	850, 872, 1287
AclWI	GGATC	7 cut(s)	479, 492, 1124, 1265, 1432, 1475, 1488
AcoI	YGGCCR	2 cut(s)	634, 721
AcuI	CTGAAG	3 cut(s)	129, 177, 1254
AcyI	GRCGYC	1 cut(s)	407
AfaI	GTAC	8 cut(s)	67, 174, 240, 989, 1761, 1791, 1901, 2003
AfeI	AGCGCT	1 cut(s)	1843
AflII	CTTAAG	1 cut(s)	1382
AhlI	ACTAGT	2 cut(s)	347, 1546
AjiI	CACGTC	1 cut(s)	502
AjnI	CCWGG	3 cut(s)	1053, 1214, 1575
AjuI	GAANNNNNNNTTGG	2 cut(s)	530, 562
Alw21I	GWGCWC	2 cut(s)	568, 1149
Alw26I	GTCTC	8 cut(s)	3, 311, 398, 990, 1370, 1468, 1608, 1921
AlwI	GGATC	7 cut(s)	479, 492, 1124, 1265, 1432, 1475, 1488
AlwNI	CAGNNNCTG	1 cut(s)	1588
Aor13HI	TCCGGA	1 cut(s)	699
Aor51HI	AGCGCT	1 cut(s)	1843
AoxI	GGCC	5 cut(s)	524, 634, 721, 882, 1064
ApaI	GGGCCC	1 cut(s)	528
ApeKI	GCWGC	6 cut(s)	198, 554, 1163, 1409, 1964, 1994
AspLEI	GCGC	1 cut(s)	1844
AspS9I	GGNCC	7 cut(s)	366, 524, 525, 883, 1023, 1064, 1111
AsuHPI	GGTGA	2 cut(s)	932, 1043
AvaII	GGWCC	3 cut(s)	366, 1023, 1111
BaeGI	GKGCMC	1 cut(s)	528
BalI	TGGCCA	2 cut(s)	636, 723
BamHI	GGATCC	2 cut(s)	484, 1480
BanI	GGYRCC	1 cut(s)	655
BanII	GRGCYC	1 cut(s)	528
BbsI	GAAGAC	4 cut(s)	504, 769, 1181, 1573
Bbv12I	GWGCWC	2 cut(s)	568, 1149
BbvI	GCAGC	6 cut(s)	210, 566, 1175, 1421, 1951, 1981
BciT130I	CCWGG	3 cut(s)	1055, 1216, 1577
BcoDI	GTCTC	8 cut(s)	3, 311, 398, 990, 1370, 1468, 1608, 1921
BcuI	ACTAGT	2 cut(s)	347, 1546
BfaI	CTAG	4 cut(s)	230, 348, 1373, 1547
BfmI	CTRYAG	3 cut(s)	69, 216, 1644
BfoI	RGCGCY	1 cut(s)	1845
BfrI	CTTAAG	1 cut(s)	1382
BglII	AGATCT	1 cut(s)	439
BisI	GCNGC	8 cut(s)	199, 555, 873, 1164, 1288, 1410, 1965, 1995
BlsI	GCNGC	8 cut(s)	200, 556, 874, 1165, 1289, 1411, 1966, 1996
BmcAI	AGTACT	1 cut(s)	67
Bme1390I	CCNGG	3 cut(s)	1055, 1216, 1577
Bme18I	GGWCC	3 cut(s)	366, 1023, 1111
BmgBI	CACGTC	1 cut(s)	502
BmgT120I	GGNCC	7 cut(s)	366, 524, 525, 883, 1023, 1064, 1111
BmiI	GGNNCC	6 cut(s)	309, 486, 526, 657, 885, 1482
BmrFI	CCNGG	3 cut(s)	1055, 1216, 1577
BmsI	GCATC	2 cut(s)	1575, 1929
BpiI	GAAGAC	4 cut(s)	504, 769, 1181, 1573
BplI	GAGNNNNNCTC	2 cut(s)	395, 427
BpmI	CTGGAG	1 cut(s)	624
BpuEI	CTTGAG	2 cut(s)	669, 1363
BsaHI	GRCGYC	1 cut(s)	407
BsaI	GGTCTC	1 cut(s)	3
BsaJI	CCNNGG	6 cut(s)	569, 659, 887, 1054, 1215, 1520
BsaWI	WCCGGW	2 cut(s)	699, 1753
BsaXI	ACNNNNNCTCC	2 cut(s)	734, 764
Bse118I	RCCGGY	1 cut(s)	1670
Bse1I	ACTGG	9 cut(s)	48, 956, 971, 1067, 1280, 1322, 1393, 1482, 1567
Bse3DI	GCAATG	3 cut(s)	186, 486, 939
BseAI	TCCGGA	1 cut(s)	699
BseBI	CCWGG	3 cut(s)	1055, 1216, 1577
BseDI	CCNNGG	6 cut(s)	569, 659, 887, 1054, 1215, 1520
BseGI	GGATG	8 cut(s)	858, 1026, 1453, 1530, 1634, 1792, 1860, 1941
BseMI	GCAATG	3 cut(s)	186, 486, 939
BseMII	CTCAG	4 cut(s)	1297, 1412, 1792, 1982
BseNI	ACTGG	9 cut(s)	48, 956, 971, 1067, 1280, 1322, 1393, 1482, 1567
BseRI	GAGGAG	2 cut(s)	1826, 1994
BseSI	GKGCMC	1 cut(s)	528
BseXI	GCAGC	6 cut(s)	210, 566, 1175, 1421, 1951, 1981
BseYI	CCCAGC	2 cut(s)	521, 1426
BsgI	GTGCAG	1 cut(s)	945
Bsh1285I	CGRYCG	2 cut(s)	1186, 1758
BshFI	GGCC	5 cut(s)	526, 636, 723, 884, 1066
BshNI	GGYRCC	1 cut(s)	655
BsiEI	CGRYCG	2 cut(s)	1186, 1758
BsiHKAI	GWGCWC	2 cut(s)	568, 1149
BsiSI	CCGG	3 cut(s)	700, 1671, 1754
BslFI	GGGAC	3 cut(s)	1436, 1759, 1970
BsmAI	GTCTC	8 cut(s)	3, 311, 398, 990, 1370, 1468, 1608, 1921
BsmBI	CGTCTC	2 cut(s)	398, 1921
BsmFI	GGGAC	3 cut(s)	1436, 1759, 1970
BsnI	GGCC	5 cut(s)	526, 636, 723, 884, 1066
Bso31I	GGTCTC	1 cut(s)	3
Bsp120I	GGGCCC	1 cut(s)	524
Bsp1286I	GDGCHC	3 cut(s)	528, 568, 1149
Bsp13I	TCCGGA	1 cut(s)	699
Bsp1407I	TGTACA	1 cut(s)	1789
Bsp143I	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
BspACI	CCGC	3 cut(s)	850, 872, 1287
BspANI	GGCC	5 cut(s)	526, 636, 723, 884, 1066
BspCNI	CTCAG	4 cut(s)	1296, 1411, 1791, 1981
BspEI	TCCGGA	1 cut(s)	699
BspHI	TCATGA	1 cut(s)	534
BspLI	GGNNCC	6 cut(s)	309, 486, 526, 657, 885, 1482
BspMAI	CTGCAG	1 cut(s)	220
BspPI	GGATC	7 cut(s)	479, 492, 1124, 1265, 1432, 1475, 1488
BspQI	GCTCTTC	1 cut(s)	423
BspT107I	GGYRCC	1 cut(s)	655
BspTI	CTTAAG	1 cut(s)	1382
BspTNI	GGTCTC	1 cut(s)	3
BsrDI	GCAATG	3 cut(s)	186, 486, 939
BsrFI	RCCGGY	1 cut(s)	1670
BsrGI	TGTACA	1 cut(s)	1789
BsrI	ACTGG	9 cut(s)	48, 956, 971, 1067, 1280, 1322, 1393, 1482, 1567
BssAI	RCCGGY	1 cut(s)	1670
BssECI	CCNNGG	6 cut(s)	569, 659, 887, 1054, 1215, 1520
BssMI	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
BssNAI	GTATAC	1 cut(s)	1017
BssNI	GRCGYC	1 cut(s)	407
BssT1I	CCWWGG	1 cut(s)	887
Bst1107I	GTATAC	1 cut(s)	1017
Bst2UI	CCWGG	3 cut(s)	1055, 1216, 1577
Bst6I	CTCTTC	2 cut(s)	87, 423
BstACI	GRCGYC	1 cut(s)	407
BstAFI	CTTAAG	1 cut(s)	1382
BstAPI	GCANNNNNTGC	1 cut(s)	563
BstAUI	TGTACA	1 cut(s)	1789
BstC8I	GCNNGC	2 cut(s)	725, 1394
BstDEI	CTNAG	5 cut(s)	1283, 1398, 1778, 1949, 1968
BstDSI	CCRYGG	2 cut(s)	569, 1520
BstF5I	GGATG	8 cut(s)	858, 1026, 1453, 1530, 1634, 1792, 1860, 1941
BstH2I	RGCGCY	1 cut(s)	1845
BstHHI	GCGC	1 cut(s)	1844
BstKTI	GATC	9 cut(s)	442, 487, 1093, 1132, 1260, 1349, 1440, 1483, 1501
BstMAI	GTCTC	8 cut(s)	3, 311, 398, 990, 1370, 1468, 1608, 1921
BstMBI	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
BstMCI	CGRYCG	2 cut(s)	1186, 1758
BstMWI	GCNNNNNNNGC	5 cut(s)	563, 856, 881, 941, 1432
BstNI	CCWGG	3 cut(s)	1055, 1216, 1577
BstNSI	RCATGY	3 cut(s)	437, 896, 1766
BstSCI	CCNGG	3 cut(s)	1053, 1214, 1575
BstSFI	CTRYAG	3 cut(s)	69, 216, 1644
BstSLI	GKGCMC	1 cut(s)	528
BstV1I	GCAGC	6 cut(s)	210, 566, 1175, 1421, 1951, 1981
BstV2I	GAAGAC	4 cut(s)	504, 769, 1181, 1573
BstX2I	RGATCY	3 cut(s)	439, 484, 1480
BstXI	CCANNNNNNTGG	1 cut(s)	1061
BstYI	RGATCY	3 cut(s)	439, 484, 1480
BstZ17I	GTATAC	1 cut(s)	1017
BsuRI	GGCC	5 cut(s)	526, 636, 723, 884, 1066
BtgI	CCRYGG	2 cut(s)	569, 1520
BtrI	CACGTC	1 cut(s)	502
BtsCI	GGATG	8 cut(s)	858, 1026, 1453, 1530, 1634, 1792, 1860, 1941
BtsIMutI	CAGTG	5 cut(s)	962, 978, 1273, 1406, 1560
Cac8I	GCNNGC	2 cut(s)	725, 1394
CaiI	CAGNNNCTG	1 cut(s)	1588
CciI	TCATGA	1 cut(s)	534
CfoI	GCGC	1 cut(s)	1844
Cfr10I	RCCGGY	1 cut(s)	1670
Cfr13I	GGNCC	7 cut(s)	366, 524, 525, 883, 1023, 1064, 1111
Csp6I	GTAC	8 cut(s)	66, 173, 239, 988, 1760, 1790, 1900, 2002
CviQI	GTAC	8 cut(s)	66, 173, 239, 988, 1760, 1790, 1900, 2002
DdeI	CTNAG	5 cut(s)	1283, 1398, 1778, 1949, 1968
DpnI	GATC	9 cut(s)	441, 486, 1092, 1131, 1259, 1348, 1439, 1482, 1500
DpnII	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
EaeI	YGGCCR	2 cut(s)	634, 721
Eam1104I	CTCTTC	2 cut(s)	87, 423
EarI	CTCTTC	2 cut(s)	87, 423
Eco130I	CCWWGG	1 cut(s)	887
Eco24I	GRGCYC	1 cut(s)	528
Eco31I	GGTCTC	1 cut(s)	3
Eco32I	GATATC	1 cut(s)	841
Eco47I	GGWCC	3 cut(s)	366, 1023, 1111
Eco47III	AGCGCT	1 cut(s)	1843
Eco57I	CTGAAG	3 cut(s)	129, 177, 1254
EcoO109I	RGGNCCY	1 cut(s)	883
EcoRII	CCWGG	3 cut(s)	1053, 1214, 1575
EcoRV	GATATC	1 cut(s)	841
EcoT14I	CCWWGG	1 cut(s)	887
EcoT22I	ATGCAT	1 cut(s)	1870
EcoT38I	GRGCYC	1 cut(s)	528
ErhI	CCWWGG	1 cut(s)	887
Esp3I	CGTCTC	2 cut(s)	398, 1921
FaqI	GGGAC	3 cut(s)	1436, 1759, 1970
FauNDI	CATATG	2 cut(s)	729, 1807
FblI	GTMKAC	2 cut(s)	1016, 1677
Fnu4HI	GCNGC	8 cut(s)	199, 555, 873, 1164, 1288, 1410, 1965, 1995
FokI	GGATG	8 cut(s)	865, 1013, 1460, 1537, 1641, 1779, 1847, 1948
FriOI	GRGCYC	1 cut(s)	528
Fsp4HI	GCNGC	8 cut(s)	199, 555, 873, 1164, 1288, 1410, 1965, 1995
FspBI	CTAG	4 cut(s)	230, 348, 1373, 1547
GlaI	GCGC	1 cut(s)	1843
GluI	GCNGC	8 cut(s)	199, 555, 873, 1164, 1288, 1410, 1965, 1995
GsaI	CCCAGC	2 cut(s)	525, 1430
GsuI	CTGGAG	1 cut(s)	624
HaeII	RGCGCY	1 cut(s)	1845
HaeIII	GGCC	5 cut(s)	526, 636, 723, 884, 1066
HapII	CCGG	3 cut(s)	700, 1671, 1754
HhaI	GCGC	1 cut(s)	1844
Hin1I	GRCGYC	1 cut(s)	407
Hin6I	GCGC	1 cut(s)	1842
HinP1I	GCGC	1 cut(s)	1842
HindIII	AAGCTT	2 cut(s)	259, 618
HpaII	CCGG	3 cut(s)	700, 1671, 1754
HphI	GGTGA	2 cut(s)	932, 1043
Hpy166II	GTNNAC	7 cut(s)	327, 458, 1017, 1678, 1715, 1775, 1830
Hpy188I	TCNGA	8 cut(s)	130, 361, 370, 417, 444, 1351, 1781, 1971
Hpy188III	TCNNGA	9 cut(s)	89, 535, 686, 700, 995, 1342, 1698, 1818, 1924
Hpy8I	GTNNAC	7 cut(s)	327, 458, 1017, 1678, 1715, 1775, 1830
HpyAV	CCTTC	7 cut(s)	30, 252, 976, 1093, 1229, 1414, 1910
HpyCH4IV	ACGT	2 cut(s)	407, 501
HpyF10VI	GCNNNNNNNGC	5 cut(s)	563, 856, 881, 941, 1432
HpyF3I	CTNAG	5 cut(s)	1283, 1398, 1778, 1949, 1968
HpySE526I	ACGT	2 cut(s)	407, 501
Hsp92I	GRCGYC	1 cut(s)	407
HspAI	GCGC	1 cut(s)	1842
Kpn2I	TCCGGA	1 cut(s)	699
Kzo9I	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
LguI	GCTCTTC	1 cut(s)	423
LmnI	GCTCC	5 cut(s)	313, 571, 605, 1144, 1423
Lsp1109I	GCAGC	6 cut(s)	210, 566, 1175, 1421, 1951, 1981
LweI	GCATC	2 cut(s)	1575, 1929
MaeI	CTAG	4 cut(s)	230, 348, 1373, 1547
MaeII	ACGT	2 cut(s)	407, 501
MaeIII	GTNAC	2 cut(s)	800, 1192
MalI	GATC	9 cut(s)	441, 486, 1092, 1131, 1259, 1348, 1439, 1482, 1500
MboI	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
MflI	RGATCY	3 cut(s)	439, 484, 1480
MhlI	GDGCHC	3 cut(s)	528, 568, 1149
MlsI	TGGCCA	2 cut(s)	636, 723
MluCI	AATT	7 cut(s)	24, 270, 670, 1121, 1302, 1458, 1876
MluNI	TGGCCA	2 cut(s)	636, 723
MlyI	GAGTC	5 cut(s)	332, 705, 779, 1770, 1823
MmeI	TCCRAC	1 cut(s)	1117
Mox20I	TGGCCA	2 cut(s)	636, 723
Mph1103I	ATGCAT	1 cut(s)	1870
MroI	TCCGGA	1 cut(s)	699
MscI	TGGCCA	2 cut(s)	636, 723
MseI	TTAA	6 cut(s)	300, 845, 1224, 1296, 1383, 1457
MslI	CAYNNNNRTG	2 cut(s)	548, 1209
Msp20I	TGGCCA	2 cut(s)	636, 723
MspA1I	CMGCKG	2 cut(s)	1166, 1287
MspCI	CTTAAG	1 cut(s)	1382
MspI	CCGG	3 cut(s)	700, 1671, 1754
MspR9I	CCNGG	3 cut(s)	1055, 1216, 1577
MvaI	CCWGG	3 cut(s)	1055, 1216, 1577
MwoI	GCNNNNNNNGC	5 cut(s)	563, 856, 881, 941, 1432
NdeI	CATATG	2 cut(s)	729, 1807
NdeII	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
NlaIV	GGNNCC	6 cut(s)	309, 486, 526, 657, 885, 1482
NmeAIII	GCCGAG	1 cut(s)	607
NmuCI	GTSAC	1 cut(s)	800
NsiI	ATGCAT	1 cut(s)	1870
NspI	RCATGY	3 cut(s)	437, 896, 1766
PagI	TCATGA	1 cut(s)	534
PciSI	GCTCTTC	1 cut(s)	423
PfeI	GAWTC	6 cut(s)	101, 119, 371, 1321, 1516, 1920
PflFI	GACNNNGTC	2 cut(s)	322, 1619
PflMI	CCANNNNNTGG	1 cut(s)	730
PkrI	GCNGC	8 cut(s)	200, 556, 874, 1165, 1289, 1411, 1966, 1996
PleI	GAGTC	5 cut(s)	331, 704, 779, 1770, 1822
PpsI	GAGTC	5 cut(s)	331, 704, 779, 1770, 1822
Psp6I	CCWGG	3 cut(s)	1053, 1214, 1575
PspFI	CCCAGC	2 cut(s)	521, 1426
PspGI	CCWGG	3 cut(s)	1053, 1214, 1575
PspN4I	GGNNCC	6 cut(s)	309, 486, 526, 657, 885, 1482
PspOMI	GGGCCC	1 cut(s)	524
PspPI	GGNCC	7 cut(s)	366, 524, 525, 883, 1023, 1064, 1111
PsrI	GAACNNNNNNTAC	2 cut(s)	441, 473
PstI	CTGCAG	1 cut(s)	220
PstNI	CAGNNNCTG	1 cut(s)	1588
PsuI	RGATCY	3 cut(s)	439, 484, 1480
PsyI	GACNNNGTC	2 cut(s)	322, 1619
PvuII	CAGCTG	1 cut(s)	1166
RsaI	GTAC	8 cut(s)	67, 174, 240, 989, 1761, 1791, 1901, 2003
RsaNI	GTAC	8 cut(s)	66, 173, 239, 988, 1760, 1790, 1900, 2002
RseI	CAYNNNNRTG	2 cut(s)	548, 1209
SapI	GCTCTTC	1 cut(s)	423
SaqAI	TTAA	6 cut(s)	300, 845, 1224, 1296, 1383, 1457
SatI	GCNGC	8 cut(s)	199, 555, 873, 1164, 1288, 1410, 1965, 1995
Sau3AI	GATC	9 cut(s)	439, 484, 1090, 1129, 1257, 1346, 1437, 1480, 1498
Sau96I	GGNCC	7 cut(s)	366, 524, 525, 883, 1023, 1064, 1111
ScaI	AGTACT	1 cut(s)	67
SchI	GAGTC	5 cut(s)	332, 705, 779, 1770, 1823
ScrFI	CCNGG	3 cut(s)	1055, 1216, 1577
SduI	GDGCHC	3 cut(s)	528, 568, 1149
SfaNI	GCATC	2 cut(s)	1575, 1929
SfcI	CTRYAG	3 cut(s)	69, 216, 1644
SinI	GGWCC	3 cut(s)	366, 1023, 1111
SmiMI	CAYNNNNRTG	2 cut(s)	548, 1209
SmlI	CTYRAG	3 cut(s)	684, 1342, 1382
SmoI	CTYRAG	3 cut(s)	684, 1342, 1382
SpeI	ACTAGT	2 cut(s)	347, 1546
Sse9I	AATT	7 cut(s)	24, 270, 670, 1121, 1302, 1458, 1876
SsiI	CCGC	3 cut(s)	850, 872, 1287
SspMI	CTAG	4 cut(s)	230, 348, 1373, 1547
StyD4I	CCNGG	3 cut(s)	1053, 1214, 1575
StyI	CCWWGG	1 cut(s)	887
TaiI	ACGT	2 cut(s)	410, 504
TaqI	TCGA	4 cut(s)	495, 575, 994, 1697
TasI	AATT	7 cut(s)	24, 270, 670, 1121, 1302, 1458, 1876
TatI	WGTACW	4 cut(s)	65, 172, 1789, 1899
TauI	GCSGC	2 cut(s)	875, 1290
TfiI	GAWTC	6 cut(s)	101, 119, 371, 1321, 1516, 1920
Tru1I	TTAA	6 cut(s)	300, 845, 1224, 1296, 1383, 1457
Tru9I	TTAA	6 cut(s)	300, 845, 1224, 1296, 1383, 1457
TscAI	CASTG	5 cut(s)	969, 978, 1280, 1406, 1567
TseFI	GTSAC	1 cut(s)	800
TseI	GCWGC	6 cut(s)	198, 554, 1163, 1409, 1964, 1994
Tsp45I	GTSAC	1 cut(s)	800
TspDTI	ATGAA	8 cut(s)	297, 438, 551, 769, 928, 1281, 1456, 1751
TspGWI	ACGGA	4 cut(s)	558, 788, 1430, 1509
TspRI	CASTG	5 cut(s)	969, 978, 1280, 1406, 1567
Tth111I	GACNNNGTC	2 cut(s)	322, 1619
Van91I	CCANNNNNTGG	1 cut(s)	730
Vha464I	CTTAAG	1 cut(s)	1382
VpaK11BI	GGWCC	3 cut(s)	366, 1023, 1111
XceI	RCATGY	3 cut(s)	437, 896, 1766
XmiI	GTMKAC	2 cut(s)	1016, 1677
XspI	CTAG	4 cut(s)	230, 348, 1373, 1547
ZraI	GACGTC	1 cut(s)	408
ZrmI	AGTACT	1 cut(s)	67
Zsp2I	ATGCAT	1 cut(s)	1870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.