MD00G1152500.v1.1

breast cancer carboxy-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012645
Physical Location & Seq
Reverse (-)
33416403 .. 33424324
7922 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1152500.v1.1.491

Sequence Viewer

Length: 3285 bp
ATGTCTCATTCTGTCGACACGTTAGGGTTTCGTTCTCCACAGTTCTCCGAGGATTTAGCTTGGCTTCCTGGTTGGCTTCAGCAGCACCAGAAAGAACAATTGGATGAATGCACCAACGAACTTAAGGGTACCAACTTAGAGCTAGCGTCCAAGGATTTGAGAAATTTTCAAGGAAACACTAGTGAAGGAAAAGATGCTAATACATTTTCACGCGAGGAAGTTGGGTACAAAAGCTGTCATTTATTCTTATCTGGGGAAGACAATTCTGCAGTTAGTTTTGCTTCATCTCCTGGAAATGTACTTCATTTCCATCTTCATCTTTCATCAAATGGATATTCACAATGTAGCCCACTTCAACCTTTGGATGCTTCTCAAAATCATATTGAATCCAATACAGTTCTGTCCGTGCAACTAAATAACACCTCAGTGGGTTCTGAATTGAAGAGCCACTCCAAAATTGGCCTAAATGTTGGTGAGATAAATTCTTTACCTCCAAAGTCCATTGAAAAACCCAGGGAGGATACTGTTCCACCCTGTCCCTCCAATAACAAGAAATCAGCAAGTCATTCTGGTGAAAAGCTTGATACCAGGTACCTGAAAGCAGCTGACATCTCTGATGCAGTTGAACTCTCCATTGCAGCATCTGAAGCATTGGTTATAAATGAAATAATGGGGAGTGGGTTGCCTTCGGACGTATTGCCAACAGCAGTGGTACTGGAAGCAGCCCTTCAAGTAAAGAAAGCAAGATTGGAGTGGCTAGATGATTCCTTAGATGGCCCAGCCGAGGAAACTGAAAATTGTGATTCTCTCTCAGATTTGGATGATTTCACCATGGCCGATGTATATAAAGATGTAGGGCTGTCTCAGAGCATACCTTCTGATGAGTGTGCATGTGATTCAGCTATTTCTCAAGTGAAAGAGACCCCTCTCTCTGGAATTCTGTATGAGTGTGTAAATCTATCTGATTCTTCAGAGCTCAGGGCTCAATGTGTCAAGTTTGATGATATCCCTATGCAAAAAGAACTAGGACAGAATTTGGTTATGGATCTCAAATCAAGAGAGAACTTCCATCCAGAATCCGTCAACTATGAGAGGGAACAGTTTCATGATAAGCTTGTTCTGGGTTCAAATATCAGTGTGGCCAGATATGATCCTTCAGCTCTTAAAAATTCAGATGGTTTTGTCATGAAGCAGACAGTTGGCTCTATGGTGGATGTAGCATCATTTCAGCATCAAAACAATGTAAACTTCCGCCCACAAGCTTGGGATTCAGGGAACTCTAAGGGAGAAGATACAGTAAGTTACTTAGCTTCGAACAGATTCAGGAGCCGGTGGTTAGGGGGTTGGACAGGCCAGGATGCATCTGCTACTCCAGAGTTGAAACAGAATACTAAAAGTGTGATGAAGTGTTTTGCTGGTGAGACGAGTTTTTTCTCAGAATCGGCAGATATTGCTCCAGACGTAAACTCTTTTGTGCAAGTGCATGACACCAAGTTTTATAGAACATCACAGTCAAGCATAGCCTTTTCTGGTTTACATGACGAAGATAACAATGGGATAATGCTCTCTCAAGATGTGGTGACATCTTCTAGCCTATCTCCAGTAGATCCTCTTTGTTCTGTTGTCCCGTGCAGTATTTCTTCAGAAAATGCTAGTCTTACATTAGCTCACAATCAGAAGGATAAGGAAAATCATAATGAAGAATGCTTTAGACCCACACTGGAACTTGCGGTGGAGAATTCACACACATCTTCAAATCCAATTATTAAATTTCCCCATGAGGACGGGCCATCTATGCCCATAATTAATGGTGAGCGTTCTCCAGTCACAGTTCGTAGGCAGATGATTTCACTCAGGACATATAGTACGCTTCTCCCAAACCTTGTTTCCATTTTTGATGGGGGAAGCTTTTATCGTGATCAGTCATTTGAACTAGAACTCGATCAGAGGCTGAATCCCTTGAATAAGGATGTCCCCTGCAATAGATCTTCTGATAAAAGGAGTTATAATGAGTCGCTTCCTTCCAATACTGTATCCTCATATTCTGCTGGAAGAGACGATGAGGGAAACAGTGAAACTACCTTAGATGCGAATCCTGTTGGAACACTGAAAAATCAGAAGAGAAGTTATCATGAGACTGAAGGAAATGGGAATGAGCTACCTATTCAAGCATTGAAAAAGAGGAGGCAGCCTCTTATTTTTAATCATAGGTCACGTTTCCGTATCCAGGCTTCTAAACCTTTAATGAACAATTCCACTGTGGAGAAACATCTCAAACTTGATTTGTTGCCGGAAAATGTTGTTAAGCTTCAACAAAACGAGGAACTCCAAACTATACAATCTGAATGCAAGAACTTCCTTGACAGAGATGTGTTAGTGAAAAAGAGAGTTCATTTCTGTGAAGCAGATATTGCAGTTCAGCTGAACAAGAACCTCCAAAAGCTAGATTCTTCAACCAAATATTGCTCAAATGCTAGAGTAAGTAAAAGATGGAAACATCCCAAGTTTCGAAGTCATGAGAGAAGTAGCTGTACAAATTGTCACCTTAAGTCTGGGAAGAGATTACTGTTTCATGGTATAGACTTCCTGCTTACAGGATTTTCTAGTCAGAAGGAAAAGGACATTGAACGAGAAATATGGAAACATGGTGGCATTGTTCTTTCTGATATTCCTTCTCCAAATTTAAGGGCAAAAAGAAGCGTACGATCCAATGGCTACCATCTTCCCGTTATTCTATGTATGAAAAAGCTACAGACCACCAAGTTTTTGTATGGTTGTGCAGTGAACTCCTTAATATTAAAAGTTGATTGGCTTACTAATTCAATTTCGGCAGGCTGTATTTTACCACCTGAGAAATACATGATACTACCGAATCGAGCTGATGCAGAGCATATCATTATCGGGGAGCCATTTCACAACTGCGAATATGTTTTTGAAAAAGTAGGAATCATGCTTCACGGAAAGCACAGTTTCTACAGCAAATTGGCAAAAATAATTAAGCACGGAGGCGGGAAGGTCTTTAAAACCCTCCAGTGGTTAGTCCACAGTTTGGACAAAGAGAAAGTTACTTTGGGAGCCATTGTTGCGGAGGATGAAAGTAGGACATCACGTCACTTGAGGCAGTGCGCGTCCGAGCAGAGGATAGCAGTGATGCCAGCTAGCTGGATCATAAAAAGCTTGTATTTAGGTAAGCTGCTTCCGTCTCCAGATAATGGCCATCCCGCTTTACCAACAATTAAGATCTCAGAGATCCCAGTTTCCGCAAAAGTGAGTGAAGATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRCT_2 RTT107_BRCT_5

Protein Analysis

1095

Amino Acids

121.74

Weight (kDa)

6.08

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT_3 PF18428 977 - 1067 5.7e-07 BRCA1 C Terminus (BRCT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 659, 2007
Acc65I GGTACC 2 cut(s) 128, 591
AccB1I GGYRCC 2 cut(s) 128, 591
AccB7I CCANNNNNTGG 2 cut(s) 3049, 3212
AccI GTMKAC 1 cut(s) 15
AccII CGCG 2 cut(s) 213, 3128
AciI CCGC 6 cut(s) 1252, 1730, 3009, 3086, 3222, 3261
AclWI GGATC 6 cut(s) 1053, 1145, 1601, 2700, 3173, 3244
AcoI YGGCCR 3 cut(s) 834, 1140, 3214
AcsI RAATTY 8 cut(s) 163, 481, 936, 1033, 1168, 1738, 1769, 2680
AcuI CTGAAG 6 cut(s) 62, 666, 954, 1140, 1626, 2160
AfaI GTAC 8 cut(s) 130, 227, 300, 593, 714, 1867, 2533, 2703
AfiI CCNNNNNNNGG 7 cut(s) 784, 932, 2227, 3033, 3049, 3138, 3212
AflII CTTAAG 2 cut(s) 122, 2546
AflIII ACRYGT 1 cut(s) 18
AhdI GACNNNNNGTC 1 cut(s) 3108
AhlI ACTAGT 1 cut(s) 179
AjiI CACGTC 1 cut(s) 3110
AjnI CCWGG 6 cut(s) 67, 289, 512, 587, 1353, 2226
AjuI GAANNNNNNNTTGG 8 cut(s) 83, 115, 731, 763, 1870, 1902, 3053, 3085
AleI CACNNNNGTG 1 cut(s) 425
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 7 cut(s) 9, 867, 914, 1415, 2049, 2129, 3207
AlwI GGATC 6 cut(s) 1053, 1145, 1601, 2700, 3173, 3244
AlwNI CAGNNNCTG 2 cut(s) 644, 1951
AoxI GGCC 7 cut(s) 460, 775, 834, 1140, 1351, 1787, 3214
ApeKI GCWGC 6 cut(s) 82, 602, 638, 722, 2188, 3193
ApoI RAATTY 8 cut(s) 163, 481, 936, 1033, 1168, 1738, 1769, 2680
AseI ATTAAT 1 cut(s) 1806
Asp700I GAANNNNTTC 2 cut(s) 1101, 1319
Asp718I GGTACC 2 cut(s) 128, 591
AspLEI GCGC 1 cut(s) 3128
AspS9I GGNCC 2 cut(s) 776, 1787
AsuHPI GGTGA 7 cut(s) 485, 584, 820, 1430, 1591, 1823, 2534
AsuII TTCGAA 2 cut(s) 1313, 2509
AsuNHI GCTAGC 2 cut(s) 142, 3158
BalI TGGCCA 2 cut(s) 1142, 3216
BanI GGYRCC 2 cut(s) 128, 591
BanII GRGCYC 2 cut(s) 978, 985
BarI GAAGNNNNNNTAC 4 cut(s) 2215, 2247, 2515, 2547
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 978
BbvI GCAGC 6 cut(s) 94, 614, 650, 734, 2200, 3180
BccI CCATC 9 cut(s) 318, 767, 1077, 1169, 1798, 1892, 2484, 2727, 3225
BciT130I CCWGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
BciVI GTATCC 3 cut(s) 514, 2044, 2234
BclI TGATCA 1 cut(s) 1918
BcoDI GTCTC 7 cut(s) 9, 867, 914, 1415, 2049, 2129, 3207
BcuI ACTAGT 1 cut(s) 179
BfmI CTRYAG 3 cut(s) 267, 2750, 2974
BfrI CTTAAG 2 cut(s) 122, 2546
BfuI GTATCC 3 cut(s) 514, 2044, 2234
BglII AGATCT 2 cut(s) 1985, 3240
BisI GCNGC 6 cut(s) 83, 603, 639, 723, 2189, 3194
BlsI GCNGC 6 cut(s) 84, 604, 640, 724, 2190, 3195
Bme1390I CCNGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
BmeRI GACNNNNNGTC 1 cut(s) 3108
BmgBI CACGTC 1 cut(s) 3110
BmgT120I GGNCC 2 cut(s) 776, 1787
BmiI GGNNCC 5 cut(s) 130, 593, 1328, 2907, 3076
BmrFI CCNGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
BmrI ACTGGG 1 cut(s) 3248
BmtI GCTAGC 2 cut(s) 146, 3162
BmuI ACTGGG 1 cut(s) 3248
BpiI GAAGAC 1 cut(s) 264
BplI GAGNNNNNCTC 2 cut(s) 2176, 2208
BpmI CTGGAG 6 cut(s) 1356, 1440, 1584, 1806, 3014, 3189
Bpu10I CCTNAGC 1 cut(s) 977
Bpu14I TTCGAA 2 cut(s) 1313, 2509
BpuEI CTTGAG 3 cut(s) 894, 1554, 3136
BsaBI GATNNNNATC 1 cut(s) 2091
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 6 cut(s) 48, 150, 512, 513, 783, 831
BsaXI ACNNNNNCTCC 2 cut(s) 3066, 3096
Bsc4I CCNNNNNNNGG 7 cut(s) 784, 932, 2227, 3033, 3049, 3138, 3212
Bse118I RCCGGY 1 cut(s) 1329
Bse1I ACTGG 6 cut(s) 720, 1601, 1725, 1823, 3031, 3254
Bse3DI GCAATG 1 cut(s) 633
Bse8I GATNNNNATC 1 cut(s) 2091
BseBI CCWGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
BseDI CCNNGG 6 cut(s) 48, 150, 512, 513, 783, 831
BseJI GATNNNNATC 1 cut(s) 2091
BseLI CCNNNNNNNGG 7 cut(s) 784, 932, 2227, 3033, 3049, 3138, 3212
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 8 cut(s) 438, 825, 878, 991, 1449, 1867, 2841, 3258
BseNI ACTGG 6 cut(s) 720, 1601, 1725, 1823, 3031, 3254
BseRI GAGGAG 1 cut(s) 2197
BseXI GCAGC 6 cut(s) 94, 614, 650, 734, 2200, 3180
BseYI CCCAGC 1 cut(s) 778
BsgI GTGCAG 2 cut(s) 1651, 2799
Bsh1236I CGCG 2 cut(s) 213, 3128
BshFI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3216
BshNI GGYRCC 2 cut(s) 128, 591
BsiHKAI GWGCWC 1 cut(s) 978
BsiSI CCGG 2 cut(s) 1330, 2291
BsiWI CGTACG 1 cut(s) 2701
BslFI GGGAC 3 cut(s) 522, 1610, 1958
BslI CCNNNNNNNGG 7 cut(s) 784, 932, 2227, 3033, 3049, 3138, 3212
BsmAI GTCTC 7 cut(s) 9, 867, 914, 1415, 2049, 2129, 3207
BsmBI CGTCTC 3 cut(s) 1415, 2049, 3207
BsmFI GGGAC 3 cut(s) 522, 1610, 1958
BsmI GAATGC 3 cut(s) 113, 1709, 2351
BsnI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3216
Bso31I GGTCTC 1 cut(s) 914
Bsp119I TTCGAA 2 cut(s) 1313, 2509
Bsp1286I GDGCHC 2 cut(s) 978, 985
Bsp1407I TGTACA 1 cut(s) 2531
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 6 cut(s) 1252, 1730, 3009, 3086, 3222, 3261
BspANI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3216
BspCNI CTCAG 8 cut(s) 437, 824, 877, 990, 1448, 1866, 2842, 3257
BspFNI CGCG 2 cut(s) 213, 3128
BspHI TCATGA 4 cut(s) 1105, 1185, 2131, 2515
BspLI GGNNCC 5 cut(s) 130, 593, 1328, 2907, 3076
BspMAI CTGCAG 1 cut(s) 271
BspOI GCTAGC 2 cut(s) 146, 3162
BspPI GGATC 6 cut(s) 1053, 1145, 1601, 2700, 3173, 3244
BspQI GCTCTTC 1 cut(s) 437
BspT104I TTCGAA 2 cut(s) 1313, 2509
BspT107I GGYRCC 2 cut(s) 128, 591
BspTI CTTAAG 2 cut(s) 122, 2546
BspTNI GGTCTC 1 cut(s) 914
BsrDI GCAATG 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 1329
BsrGI TGTACA 1 cut(s) 2531
BsrI ACTGG 6 cut(s) 720, 1601, 1725, 1823, 3031, 3254
BssAI RCCGGY 1 cut(s) 1329
BssECI CCNNGG 6 cut(s) 48, 150, 512, 513, 783, 831
BssT1I CCWWGG 2 cut(s) 150, 831
Bst2UI CCWGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
Bst6I CTCTTC 4 cut(s) 437, 2047, 2114, 2552
BstAFI CTTAAG 2 cut(s) 122, 2546
BstAPI GCANNNNNTGC 2 cut(s) 1451, 2411
BstAUI TGTACA 1 cut(s) 2531
BstBI TTCGAA 2 cut(s) 1313, 2509
BstC8I GCNNGC 4 cut(s) 144, 2833, 3156, 3160
BstDSI CCRYGG 1 cut(s) 831
BstFNI CGCG 2 cut(s) 213, 3128
BstHHI GCGC 1 cut(s) 3128
BstMAI GTCTC 7 cut(s) 9, 867, 914, 1415, 2049, 2129, 3207
BstMWI GCNNNNNNNGC 6 cut(s) 82, 647, 1451, 1795, 2411, 3083
BstNI CCWGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
BstNSI RCATGY 1 cut(s) 894
BstSCI CCNGG 6 cut(s) 67, 289, 512, 587, 1353, 2226
BstSFI CTRYAG 3 cut(s) 267, 2750, 2974
BstUI CGCG 2 cut(s) 213, 3128
BstV1I GCAGC 6 cut(s) 94, 614, 650, 734, 2200, 3180
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 5 cut(s) 1045, 1606, 1985, 3240, 3249
BstXI CCANNNNNNTGG 2 cut(s) 1263, 3162
BstYI RGATCY 5 cut(s) 1045, 1606, 1985, 3240, 3249
BsuI GTATCC 3 cut(s) 514, 2044, 2234
BsuRI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3216
BtgI CCRYGG 1 cut(s) 831
BtrI CACGTC 1 cut(s) 3110
BtsI GCAGTG 4 cut(s) 714, 2787, 3128, 3153
Cac8I GCNNGC 4 cut(s) 144, 2833, 3156, 3160
CaiI CAGNNNCTG 2 cut(s) 644, 1951
CciI TCATGA 4 cut(s) 1105, 1185, 2131, 2515
CfoI GCGC 1 cut(s) 3128
Cfr10I RCCGGY 1 cut(s) 1329
Cfr13I GGNCC 2 cut(s) 776, 1787
CseI GACGC 2 cut(s) 135, 3117
CsiI ACCWGGT 1 cut(s) 587
Csp6I GTAC 8 cut(s) 129, 226, 299, 592, 713, 1866, 2532, 2702
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviQI GTAC 8 cut(s) 129, 226, 299, 592, 713, 1866, 2532, 2702
DraI TTTAAA 1 cut(s) 3022
DriI GACNNNNNGTC 1 cut(s) 3108
EaeI YGGCCR 3 cut(s) 834, 1140, 3214
Eam1104I CTCTTC 4 cut(s) 437, 2047, 2114, 2552
Eam1105I GACNNNNNGTC 1 cut(s) 3108
EarI CTCTTC 4 cut(s) 437, 2047, 2114, 2552
EciI GGCGGA 1 cut(s) 1241
Ecl136II GAGCTC 1 cut(s) 976
Eco130I CCWWGG 2 cut(s) 150, 831
Eco24I GRGCYC 2 cut(s) 978, 985
Eco31I GGTCTC 1 cut(s) 914
Eco32I GATATC 1 cut(s) 1006
Eco53kI GAGCTC 1 cut(s) 976
Eco57I CTGAAG 6 cut(s) 62, 666, 954, 1140, 1626, 2160
EcoICRI GAGCTC 1 cut(s) 976
EcoRI GAATTC 2 cut(s) 936, 1738
EcoRII CCWGG 6 cut(s) 67, 289, 512, 587, 1353, 2226
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 2 cut(s) 150, 831
EcoT22I ATGCAT 1 cut(s) 1363
EcoT38I GRGCYC 2 cut(s) 978, 985
ErhI CCWWGG 2 cut(s) 150, 831
Esp3I CGTCTC 3 cut(s) 1415, 2049, 3207
FalI AAGNNNNNCTT 2 cut(s) 711, 743
FaqI GGGAC 3 cut(s) 522, 1610, 1958
FauI CCCGC 2 cut(s) 3002, 3229
FbaI TGATCA 1 cut(s) 1918
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 6 cut(s) 83, 603, 639, 723, 2189, 3194
FriOI GRGCYC 2 cut(s) 978, 985
Fsp4HI GCNGC 6 cut(s) 83, 603, 639, 723, 2189, 3194
GlaI GCGC 1 cut(s) 3127
GluI GCNGC 6 cut(s) 83, 603, 639, 723, 2189, 3194
GsaI CCCAGC 1 cut(s) 782
GsuI CTGGAG 6 cut(s) 1356, 1440, 1584, 1806, 3014, 3189
HaeIII GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3216
HapII CCGG 2 cut(s) 1330, 2291
HgaI GACGC 2 cut(s) 135, 3117
HhaI GCGC 1 cut(s) 3128
Hin6I GCGC 1 cut(s) 3126
HinP1I GCGC 1 cut(s) 3126
HincII GTYRAC 2 cut(s) 16, 1084
HindII GTYRAC 2 cut(s) 16, 1084
HindIII AAGCTT 6 cut(s) 578, 1112, 1260, 1906, 2306, 3175
HpaII CCGG 2 cut(s) 1330, 2291
HphI GGTGA 7 cut(s) 485, 584, 820, 1430, 1591, 1823, 2534
Hpy166II GTNNAC 7 cut(s) 16, 1084, 1246, 1465, 1535, 2785, 3043
Hpy8I GTNNAC 7 cut(s) 16, 1084, 1246, 1465, 1535, 2785, 3043
HpyCH4IV ACGT 5 cut(s) 20, 693, 1461, 2215, 3109
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 647, 1451, 1795, 2411, 3083
HpySE526I ACGT 5 cut(s) 20, 693, 1461, 2215, 3109
HspAI GCGC 1 cut(s) 3126
KpnI GGTACC 2 cut(s) 132, 595
Ksp22I TGATCA 1 cut(s) 1918
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 4 cut(s) 1326, 1459, 2905, 3074
Lsp1109I GCAGC 6 cut(s) 94, 614, 650, 734, 2200, 3180
MabI ACCWGGT 1 cut(s) 587
MaeII ACGT 5 cut(s) 20, 693, 1461, 2215, 3109
MaeIII GTNAC 7 cut(s) 1301, 1579, 1825, 2211, 2540, 3064, 3110
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 5 cut(s) 1045, 1606, 1985, 3240, 3249
MhlI GDGCHC 2 cut(s) 978, 985
MlsI TGGCCA 2 cut(s) 1142, 3216
MluNI TGGCCA 2 cut(s) 1142, 3216
MlyI GAGTC 1 cut(s) 2021
MmeI TCCRAC 2 cut(s) 1325, 2080
Mox20I TGGCCA 2 cut(s) 1142, 3216
Mph1103I ATGCAT 1 cut(s) 1363
MroXI GAANNNNTTC 2 cut(s) 1101, 1319
MscI TGGCCA 2 cut(s) 1142, 3216
MslI CAYNNNNRTG 3 cut(s) 425, 570, 2397
Msp20I TGGCCA 2 cut(s) 1142, 3216
MspA1I CMGCKG 2 cut(s) 605, 2422
MspCI CTTAAG 2 cut(s) 122, 2546
MspI CCGG 2 cut(s) 1330, 2291
MspR9I CCNGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 3 cut(s) 113, 1709, 2351
MvaI CCWGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
MvnI CGCG 2 cut(s) 213, 3128
MwoI GCNNNNNNNGC 6 cut(s) 82, 647, 1451, 1795, 2411, 3083
NcoI CCATGG 1 cut(s) 831
NheI GCTAGC 2 cut(s) 142, 3158
NlaIV GGNNCC 5 cut(s) 130, 593, 1328, 2907, 3076
NmeAIII GCCGAG 1 cut(s) 808
NmuCI GTSAC 5 cut(s) 1579, 1825, 2211, 2540, 3110
NsiI ATGCAT 1 cut(s) 1363
NspI RCATGY 1 cut(s) 894
NspV TTCGAA 2 cut(s) 1313, 2509
OliI CACNNNNGTG 1 cut(s) 425
PagI TCATGA 4 cut(s) 1105, 1185, 2131, 2515
PasI CCCWGGG 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 437
PctI GAATGC 3 cut(s) 113, 1709, 2351
PdmI GAANNNNTTC 2 cut(s) 1101, 1319
Pfl23II CGTACG 1 cut(s) 2701
PflMI CCANNNNNTGG 2 cut(s) 3049, 3212
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 6 cut(s) 84, 604, 640, 724, 2190, 3195
PleI GAGTC 1 cut(s) 2020
PpsI GAGTC 1 cut(s) 2020
PshBI ATTAAT 1 cut(s) 1806
PsiI TTATAA 2 cut(s) 659, 2007
Psp124BI GAGCTC 1 cut(s) 978
Psp6I CCWGG 6 cut(s) 67, 289, 512, 587, 1353, 2226
PspFI CCCAGC 1 cut(s) 778
PspGI CCWGG 6 cut(s) 67, 289, 512, 587, 1353, 2226
PspLI CGTACG 1 cut(s) 2701
PspN4I GGNNCC 5 cut(s) 130, 593, 1328, 2907, 3076
PspPI GGNCC 2 cut(s) 776, 1787
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 2 cut(s) 644, 1951
PsuI RGATCY 5 cut(s) 1045, 1606, 1985, 3240, 3249
PvuII CAGCTG 2 cut(s) 605, 2422
RsaI GTAC 8 cut(s) 130, 227, 300, 593, 714, 1867, 2533, 2703
RsaNI GTAC 8 cut(s) 129, 226, 299, 592, 713, 1866, 2532, 2702
RseI CAYNNNNRTG 3 cut(s) 425, 570, 2397
SacI GAGCTC 1 cut(s) 978
SalI GTCGAC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 437
SatI GCNGC 6 cut(s) 83, 603, 639, 723, 2189, 3194
Sau96I GGNCC 2 cut(s) 776, 1787
SchI GAGTC 1 cut(s) 2021
ScrFI CCNGG 6 cut(s) 69, 291, 514, 589, 1355, 2228
SduI GDGCHC 2 cut(s) 978, 985
SexAI ACCWGGT 1 cut(s) 587
SfcI CTRYAG 3 cut(s) 267, 2750, 2974
SfuI TTCGAA 2 cut(s) 1313, 2509
SmiMI CAYNNNNRTG 3 cut(s) 425, 570, 2397
SmlI CTYRAG 5 cut(s) 122, 909, 1569, 2546, 3115
SmoI CTYRAG 5 cut(s) 122, 909, 1569, 2546, 3115
SpeI ACTAGT 1 cut(s) 179
SsiI CCGC 6 cut(s) 1252, 1730, 3009, 3086, 3222, 3261
SspI AATATT 2 cut(s) 2462, 2796
SstI GAGCTC 1 cut(s) 978
StyD4I CCNGG 6 cut(s) 67, 289, 512, 587, 1353, 2226
StyI CCWWGG 2 cut(s) 150, 831
TaiI ACGT 5 cut(s) 23, 696, 1464, 2218, 3112
TaqI TCGA 5 cut(s) 15, 1313, 1941, 2509, 2875
TatI WGTACW 2 cut(s) 298, 2531
TseFI GTSAC 5 cut(s) 1579, 1825, 2211, 2540, 3110
TseI GCWGC 6 cut(s) 82, 602, 638, 722, 2188, 3193
Tsp45I GTSAC 5 cut(s) 1579, 1825, 2211, 2540, 3110
TspGWI ACGGA 6 cut(s) 394, 1069, 2210, 2973, 3018, 3189
Van91I CCANNNNNTGG 2 cut(s) 3049, 3212
Vha464I CTTAAG 2 cut(s) 122, 2546
VspI ATTAAT 1 cut(s) 1806
XapI RAATTY 8 cut(s) 163, 481, 936, 1033, 1168, 1738, 1769, 2680
XceI RCATGY 1 cut(s) 894
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 15
XmnI GAANNNNTTC 2 cut(s) 1101, 1319
Zsp2I ATGCAT 1 cut(s) 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.