Rorug01G0068200

breast cancer carboxy-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012645
Physical Location & Seq
Forward (+)
11127842 .. 11130677
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0068200.1

Sequence Viewer

Length: 1944 bp
ATGCTAACCTCCAATCCCTCAAAGCTTCCCCCATTCTCTTCTCTCTCCCATCTTCTTCGAACTCTCTCATCCATTGCTGTTCCATCTCACAACCACATATCACATCTTCTACTCGAACAAAAATCAGCCACCCAGGTCCTCGACACCTTCCAATGGGCCTCCAAACTCCCCAGCTTCACCCACTCCCCGTCCACTTACCGAGCCCTCATCCACAAGCTATGCACCTTTCGCCGCTTCGACATTGCCCACCAACTGCTCGACGAAATGCCCCACTCAATCGGCCTACCCCCAGATGACCACATTTTCGTCACACTCATCCACGGCCTCGGCCGAGCCCATTTGGTGAAGCAAGTGATCAAGGTGCTTGACTTGGTTCGCAAGTATAACCAGAGACCTTCTCTCAAGATATTCAATTCAATACTCGATGTTCTTGTGAAGGAGGATATAGATATAGCTAGGGAGTTTTATAGGAAGAAGATGGTGGCAAGTGGCGTCAGGGGTGATGATTATACTTTTGGTATCTTGATGAAAGGTCTTTGTTTGACGAATCGGATTGGTGATGGGTTTAAGCTTTTGCAGGCTATTAAGTCGAGGGGAGTTGCGCCGAATACTGTGGTTTTTAACACATTGCTGCATGCACTTTGCAGGAATGGGAAAGTTGGGAGAGCCAGAAGCTTGATGAATGAGATGGAAGACCCCAATGATGTGACTTTTAATATTTTGATATCTGGTTATTGTGGAGAAGAGAATTTAGTGCAGGCGCTTGTTATGTTAGAGAAGTGTTTTGGTCTGGGGTATGTGCCTGATGTTGTCACGACAACCAAGGTGTTGGAAATTCTGTGCAATGATGGTCGGGTAATGGAGGCTGTCAGGGTTATAGAGAGAGTGGAGAGCAATGGAGGGTTGGTTGATGTTGTGGCATATAATACCTTGATTAAGGGTTACTGTCGGTTAGGGAAAGCAAAACTTGGTCTTCGCATTTTGAAGGAGATAGAGAGAAAAGGGTGTCTTCCAAATGTTGATACCTACAATGTCTTGATCTCGGGTTTTTGTGAGTCTGGGATGTTGGATAAAGCTCTTGATCTGTTTAATGATATGAAAACAGATGGCATCAATTGGAATTTTGCTACATATGATACGTTAATAAGAGGCTTGTGTTCAGGAGGAAGAACTGAAGATGGGTTAAAAATTTTGGATTTGATGAATGAGAGCAAAGATGGTTGTAGGGGTCGAATAGGTCCTTTTAATAGTGTCTTGTATGGCATGTATAAGGAAAAACGTTTGGACGAAGCACTTGAGTTTCTTGCCAATATGGGTAAACTATTTCCCAGAGCTGTTGATAGGAGCTTTAGGATTTTAGGTTTCTGTGAGGAGGGTGCCACTGAGAATGCAAAGAAGGTCTACGATCAGATGATTGTGGAAAGGGCAGTCCCATGTGTTTTGATTTATGACTATCTAATCCATAGATTTTGCCAACAAGGGTGTGTTCGTCAAGCCTTTGAGCTTATGAATGAGATGATTGCTCATTGTTACTTTCCTGTTTCATCGACTTTCAATGTTCTGATTAACGGGTTTTGTGAGCAAGGAAAAGTTGGTAGTGCGCTAAAGCTCCTCAATGAGATGATTGGTAGAGGTTGTTTACCCGATATAGAAAGTTATAGTCATTTGGTAGGTGCTCTTTGCCAGAAGGGGGATTTTCAGAAAGCTTTAAAACTCGTTTTGCAAATGGTAGGAAAGGGTATCACTCCTGATTATTTCACATGGAACTCGTTGCTTCTTTGTTCAAGTCAAGAGACAGTATGGTTAAACAGCAAAATACTACTGAGAGGTTACTCACTATGTTCAGGTCTGTGGCTCAGTCCCTTTCTTATCCCAATACCGGGAAGCTTAAATGGTCTGGGATCCTCCAGAGGGATGTTATCCTCTGTTAAGTTCAAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRCT_2 RTT107_BRCT_5

Protein Analysis

647

Amino Acids

72.29

Weight (kDa)

8.71

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 190 - 246 2.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 198 - 231 9.3e-11 PPR repeat
PPR_2 PF13041 202 - 246 8.9e-14 PPR repeat family
PPR_1 PF12854 233 - 260 4.3e-06 PPR repeat
TPR_24 PF23276 234 - 330 7.9e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 301 - 330 7e-06 PPR repeat
PPR_3 PF13812 303 - 349 1.3e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 304 - 351 5.9e-17 PPR repeat family
PPR PF01535 306 - 336 6.3e-07 PPR repeat
PPR_1 PF12854 334 - 367 1.1e-13 PPR repeat
PPR_2 PF13041 338 - 386 4.2e-17 PPR repeat family
PPR PF01535 342 - 371 5.7e-10 PPR repeat
PPR_long PF17177 462 - 595 8.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 511 - 541 2.9e-09 PPR repeat
PPR_2 PF13041 515 - 562 8.5e-14 PPR repeat family
PPR PF01535 516 - 546 7.9e-08 PPR repeat
PPR_3 PF13812 536 - 592 6.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 544 - 577 3.5e-06 PPR repeat
PPR_2 PF13041 548 - 592 2.4e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1376
AccI GTMKAC 1 cut(s) 1401
AciI CCGC 1 cut(s) 232
AclI AACGTT 1 cut(s) 1279
AclWI GGATC 2 cut(s) 1896, 1909
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 4 cut(s) 748, 834, 1120, 1188
AcuI CTGAAG 1 cut(s) 1194
AcyI GRCGYC 1 cut(s) 492
AfiI CCNNNNNNNGG 5 cut(s) 153, 1690, 1879, 1880, 1911
AgsI TTSAA 6 cut(s) 412, 417, 985, 1555, 1785, 1936
AjnI CCWGG 1 cut(s) 132
Alw21I GWGCWC 1 cut(s) 1678
Alw26I GTCTC 2 cut(s) 385, 1787
AlwI GGATC 2 cut(s) 1896, 1909
Ama87I CYCGRG 1 cut(s) 1042
AoxI GGCC 4 cut(s) 156, 280, 322, 328
ApeKI GCWGC 1 cut(s) 631
ApoI RAATTY 4 cut(s) 748, 834, 1120, 1188
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspLEI GCGC 3 cut(s) 604, 763, 1603
AspS9I GGNCC 3 cut(s) 136, 156, 1238
AsuC2I CCSGG 1 cut(s) 1881
AsuHPI GGTGA 4 cut(s) 169, 355, 512, 569
AsuII TTCGAA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 1042
AvaII GGWCC 2 cut(s) 136, 1238
BamHI GGATCC 1 cut(s) 1901
BanI GGYRCC 1 cut(s) 1376
BanII GRGCYC 2 cut(s) 205, 337
BbsI GAAGAC 3 cut(s) 699, 965, 1001
Bbv12I GWGCWC 1 cut(s) 1678
BbvI GCAGC 1 cut(s) 618
BccI CCATC 9 cut(s) 57, 91, 472, 554, 682, 842, 1100, 1172, 1211
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 1 cut(s) 134
BclI TGATCA 1 cut(s) 354
BcnI CCSGG 1 cut(s) 1881
BcoDI GTCTC 2 cut(s) 385, 1787
BfaI CTAG 1 cut(s) 456
BfoI RGCGCY 1 cut(s) 764
BisI GCNGC 2 cut(s) 232, 632
BlsI GCNGC 2 cut(s) 233, 633
Bme1390I CCNGG 2 cut(s) 134, 1881
Bme18I GGWCC 2 cut(s) 136, 1238
BmeT110I CYCGRG 1 cut(s) 1042
BmgT120I GGNCC 3 cut(s) 136, 156, 1238
BmiI GGNNCC 2 cut(s) 1378, 1903
BmrFI CCNGG 2 cut(s) 134, 1881
BmsI GCATC 1 cut(s) 1119
BpiI GAAGAC 3 cut(s) 699, 965, 1001
BplI GAGNNNNNCTC 2 cut(s) 382, 414
BpmI CTGGAG 1 cut(s) 1891
Bpu14I TTCGAA 1 cut(s) 58
BpuEI CTTGAG 2 cut(s) 386, 1316
BpuMI CCSGG 1 cut(s) 1881
BsaHI GRCGYC 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 385
BsaJI CCNNGG 4 cut(s) 132, 319, 325, 822
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 5 cut(s) 153, 1690, 1879, 1880, 1911
Bse3DI GCAATG 5 cut(s) 72, 240, 626, 850, 901
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 4 cut(s) 132, 319, 325, 822
BseGI GGATG 5 cut(s) 68, 207, 315, 1068, 1920
BseLI CCNNNNNNNGG 5 cut(s) 153, 1690, 1879, 1880, 1911
BseMI GCAATG 5 cut(s) 72, 240, 626, 850, 901
BseMII CTCAG 3 cut(s) 1374, 1814, 1870
BseRI GAGGAG 2 cut(s) 1385, 1601
BseX3I CGGCCG 1 cut(s) 328
BseXI GCAGC 1 cut(s) 618
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 776
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 4 cut(s) 158, 282, 324, 330
BshNI GGYRCC 1 cut(s) 1376
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 1678
BsiHKCI CYCGRG 1 cut(s) 1042
BsiSI CCGG 1 cut(s) 1880
BslFI GGGAC 2 cut(s) 1415, 1845
BslI CCNNNNNNNGG 5 cut(s) 153, 1690, 1879, 1880, 1911
BsmAI GTCTC 2 cut(s) 385, 1787
BsmFI GGGAC 2 cut(s) 1415, 1845
BsmI GAATGC 1 cut(s) 1393
BsnI GGCC 4 cut(s) 158, 282, 324, 330
Bso31I GGTCTC 1 cut(s) 385
BsoBI CYCGRG 1 cut(s) 1042
Bsp119I TTCGAA 1 cut(s) 58
Bsp1286I GDGCHC 3 cut(s) 205, 337, 1678
Bsp143I GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
BspACI CCGC 1 cut(s) 232
BspANI GGCC 4 cut(s) 158, 282, 324, 330
BspCNI CTCAG 3 cut(s) 1375, 1815, 1869
BspLI GGNNCC 2 cut(s) 1378, 1903
BspPI GGATC 2 cut(s) 1896, 1909
BspT104I TTCGAA 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 1376
BspTNI GGTCTC 1 cut(s) 385
BsrDI GCAATG 5 cut(s) 72, 240, 626, 850, 901
BssECI CCNNGG 4 cut(s) 132, 319, 325, 822
BssMI GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
BssNI GRCGYC 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 822
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 3 cut(s) 613, 947, 1798
Bst6I CTCTTC 2 cut(s) 43, 738
BstACI GRCGYC 1 cut(s) 492
BstBI TTCGAA 1 cut(s) 58
BstC8I GCNNGC 3 cut(s) 579, 636, 759
BstDEI CTNAG 3 cut(s) 1383, 1823, 1856
BstDSI CCRYGG 1 cut(s) 319
BstF5I GGATG 5 cut(s) 68, 207, 315, 1068, 1920
BstH2I RGCGCY 1 cut(s) 764
BstHHI GCGC 3 cut(s) 604, 763, 1603
BstKTI GATC 5 cut(s) 357, 1041, 1084, 1408, 1904
BstMAI GTCTC 2 cut(s) 385, 1787
BstMBI GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 1 cut(s) 228
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 2 cut(s) 638, 1267
BstSCI CCNGG 2 cut(s) 132, 1879
BstV1I GCAGC 1 cut(s) 618
BstV2I GAAGAC 3 cut(s) 699, 965, 1001
BstX2I RGATCY 1 cut(s) 1901
BstXI CCANNNNNNTGG 1 cut(s) 829
BstYI RGATCY 1 cut(s) 1901
BstZI CGGCCG 1 cut(s) 328
BsuRI GGCC 4 cut(s) 158, 282, 324, 330
BtgI CCRYGG 1 cut(s) 319
BtsCI GGATG 5 cut(s) 68, 207, 315, 1068, 1920
BtsIMutI CAGTG 1 cut(s) 1380
Cac8I GCNNGC 3 cut(s) 579, 636, 759
CfoI GCGC 3 cut(s) 604, 763, 1603
Cfr13I GGNCC 3 cut(s) 136, 156, 1238
CseI GACGC 1 cut(s) 481
CviAII CATG 4 cut(s) 635, 1264, 1434, 1761
DdeI CTNAG 3 cut(s) 1383, 1823, 1856
DpnI GATC 5 cut(s) 356, 1040, 1083, 1407, 1903
DpnII GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
DraI TTTAAA 1 cut(s) 1710
EaeI YGGCCR 1 cut(s) 328
EagI CGGCCG 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 43, 738
EarI CTCTTC 2 cut(s) 43, 738
EclXI CGGCCG 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 822
Eco24I GRGCYC 2 cut(s) 205, 337
Eco31I GGTCTC 1 cut(s) 385
Eco32I GATATC 1 cut(s) 726
Eco47I GGWCC 2 cut(s) 136, 1238
Eco52I CGGCCG 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 1194
Eco88I CYCGRG 1 cut(s) 1042
EcoO109I RGGNCCY 2 cut(s) 136, 1238
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 726
EcoT14I CCWWGG 1 cut(s) 822
EcoT38I GRGCYC 2 cut(s) 205, 337
ErhI CCWWGG 1 cut(s) 822
FaeI CATG 4 cut(s) 638, 1267, 1437, 1764
FalI AAGNNNNNCTT 4 cut(s) 951, 983, 993, 1025
FaqI GGGAC 2 cut(s) 1415, 1845
FatI CATG 4 cut(s) 634, 1263, 1433, 1760
FauNDI CATATG 1 cut(s) 1132
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 1401
Fnu4HI GCNGC 2 cut(s) 232, 632
FokI GGATG 5 cut(s) 55, 194, 302, 1075, 1927
FriOI GRGCYC 2 cut(s) 205, 337
Fsp4HI GCNGC 2 cut(s) 232, 632
FspBI CTAG 1 cut(s) 456
GlaI GCGC 3 cut(s) 603, 762, 1602
GluI GCNGC 2 cut(s) 232, 632
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 1891
HaeII RGCGCY 1 cut(s) 764
HaeIII GGCC 4 cut(s) 158, 282, 324, 330
HapII CCGG 1 cut(s) 1880
HgaI GACGC 1 cut(s) 481
HhaI GCGC 3 cut(s) 604, 763, 1603
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 4 cut(s) 638, 1267, 1437, 1764
Hin6I GCGC 3 cut(s) 602, 761, 1601
HinP1I GCGC 3 cut(s) 602, 761, 1601
HindIII AAGCTT 5 cut(s) 23, 569, 673, 1704, 1885
HinfI GANTC 2 cut(s) 547, 1055
HpaII CCGG 1 cut(s) 1880
HphI GGTGA 4 cut(s) 169, 355, 512, 569
Hpy166II GTNNAC 4 cut(s) 192, 1319, 1402, 1640
Hpy188I TCNGA 4 cut(s) 552, 1410, 1563, 1701
Hpy188III TCNNGA 9 cut(s) 403, 523, 814, 1036, 1079, 1161, 1748, 1790, 1908
Hpy8I GTNNAC 4 cut(s) 192, 1319, 1402, 1640
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 6 cut(s) 157, 405, 430, 979, 1390, 1681
HpyCH4III ACNGT 3 cut(s) 613, 947, 1798
HpyCH4IV ACGT 2 cut(s) 1139, 1279
HpyCH4V TGCA 9 cut(s) 222, 577, 634, 638, 645, 757, 843, 1391, 1723
HpyF10VI GCNNNNNNNGC 1 cut(s) 228
HpyF3I CTNAG 3 cut(s) 1383, 1823, 1856
HpySE526I ACGT 2 cut(s) 1139, 1279
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 4 cut(s) 638, 1267, 1437, 1764
HspAI GCGC 3 cut(s) 602, 761, 1601
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
LmnI GCTCC 2 cut(s) 1344, 1614
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 1 cut(s) 1119
MaeI CTAG 1 cut(s) 456
MaeII ACGT 2 cut(s) 1139, 1279
MaeIII GTNAC 6 cut(s) 307, 706, 811, 941, 1529, 1829
MalI GATC 5 cut(s) 356, 1040, 1083, 1407, 1903
MboI GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
MfeI CAATTG 1 cut(s) 1114
MflI RGATCY 1 cut(s) 1901
MhlI GDGCHC 3 cut(s) 205, 337, 1678
MluCI AATT 6 cut(s) 412, 748, 834, 1114, 1120, 1188
MlyI GAGTC 1 cut(s) 1064
MmeI TCCRAC 2 cut(s) 810, 1047
MspI CCGG 1 cut(s) 1880
MspR9I CCNGG 2 cut(s) 134, 1881
MunI CAATTG 1 cut(s) 1114
Mva1269I GAATGC 1 cut(s) 1393
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 228
NciI CCSGG 1 cut(s) 1881
NdeI CATATG 1 cut(s) 1132
NdeII GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
NlaIII CATG 4 cut(s) 638, 1267, 1437, 1764
NlaIV GGNNCC 2 cut(s) 1378, 1903
NmeAIII GCCGAG 2 cut(s) 306, 356
NmuCI GTSAC 3 cut(s) 307, 706, 811
NspI RCATGY 2 cut(s) 638, 1267
NspV TTCGAA 1 cut(s) 58
PaeI GCATGC 1 cut(s) 638
PctI GAATGC 1 cut(s) 1393
PfeI GAWTC 1 cut(s) 547
PkrI GCNGC 2 cut(s) 233, 633
PleI GAGTC 1 cut(s) 1063
PpsI GAGTC 1 cut(s) 1063
PpuMI RGGWCCY 2 cut(s) 136, 1238
Psp1406I AACGTT 1 cut(s) 1279
Psp5II RGGWCCY 2 cut(s) 136, 1238
Psp6I CCWGG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 2 cut(s) 1378, 1903
PspPI GGNCC 3 cut(s) 136, 156, 1238
PspPPI RGGWCCY 2 cut(s) 136, 1238
PsuI RGATCY 1 cut(s) 1901
SatI GCNGC 2 cut(s) 232, 632
Sau3AI GATC 5 cut(s) 354, 1038, 1081, 1405, 1901
Sau96I GGNCC 3 cut(s) 136, 156, 1238
SchI GAGTC 1 cut(s) 1064
ScrFI CCNGG 2 cut(s) 134, 1881
SduI GDGCHC 3 cut(s) 205, 337, 1678
SfaNI GCATC 1 cut(s) 1119
SfuI TTCGAA 1 cut(s) 58
SinI GGWCC 2 cut(s) 136, 1238
SmlI CTYRAG 2 cut(s) 401, 1295
SmoI CTYRAG 2 cut(s) 401, 1295
SphI GCATGC 1 cut(s) 638
Sse9I AATT 6 cut(s) 412, 748, 834, 1114, 1120, 1188
SsiI CCGC 1 cut(s) 232
SspI AATATT 1 cut(s) 718
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 132, 1879
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 3 cut(s) 613, 947, 1798
TaiI ACGT 2 cut(s) 1142, 1282
TaqI TCGA 9 cut(s) 58, 114, 141, 237, 258, 423, 590, 1231, 1547
TasI AATT 6 cut(s) 412, 748, 834, 1114, 1120, 1188
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 547
TscAI CASTG 1 cut(s) 1387
TseFI GTSAC 3 cut(s) 307, 706, 811
TseI GCWGC 1 cut(s) 631
Tsp45I GTSAC 3 cut(s) 307, 706, 811
TspDTI ATGAA 6 cut(s) 542, 695, 1112, 1217, 1523, 1533
TspRI CASTG 1 cut(s) 1387
VpaK11BI GGWCC 2 cut(s) 136, 1238
XapI RAATTY 4 cut(s) 748, 834, 1120, 1188
XceI RCATGY 2 cut(s) 638, 1267
XmiI GTMKAC 1 cut(s) 1401
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.