MD00G1164700.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015802
Physical Location & Seq
Forward (+)
37470144 .. 37474227
4084 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1164700.v1.1.491

Sequence Viewer

Length: 3444 bp
ATGTTTACAATGCTACCGCCCAGCTCGAGGCAATCCTTCTCCTTCTACACCTCCTCCCACTCTTATGTTTTTCTCTGTCTCACCCTTTTTTTTCTTTACACACCCGCCTTTGCCTCAGCCGGAAACCCCGAAGCCACCCTTCTCTTCTCCTGGCTCCACTCCTCTGCTTCGCCTCCACCCTCCTTCTTCTCCAACTGGAACCTCCTCCACTCCAATCCATGCAACTGGTCCGCCATATCATGTTCTTCTCAAGGCCACGTCACCGAAATCACAATCCAATCCATTCCTCTAGAACTCCCACTCCCTTCCAATCTCTCTTCCTTTCCCTCCCTCCAAAAACTCATCATTTCCGGCGCTAATCTCACCGGAACCATCCCCTCCGACGTCGGCGACTGCACTGAGCTGAAAGTCATCGACCTCAGCTCAAACTCTCTGGTGGGTTCAATCCCATCTACCTTTTCGAGGCTCCGAAATCTCCAAGACTTGATCTTGAATTCCAACCAGCTAACTGGGAAAATCCCAGTCGAGCTGAGCACCTGCATTGGACTAAAAAACCTTCTTATTTTCGACAACCAGTTAACCGGGTCGATCCCGCCGGAGCTCGGGAAGCTCACCGGCCTTGAAGTGCTGAGAGCAGGAGGGAACAAGTACATTGGTGGGAAAATCCCGGAAGAGCTCGGAGGCTGCAGCAATTTGACTGTCTTAGGGTTGGCAGACACCCAAGTTTCAGGCACTTTGCCTGCCTCATTGGGAAAGCTTAGCAATCTCCAAACCCTGTCTATTTACACCACAATGATCTCTGGTGAAATCCCACCTGAAATAGGTAAATGCTCTGAGCTTGTAAGTTTGTTTCTTTACGAAAATAGCCTCTCCGGTTCGATCCCGCGTGATCTCGGGAAGCTTAAGAAGCTGCAGCAGTTGCTGCTGTGGCAAAATAGTCTTTTTGGTGCAATCCCAGAAGAGATTGGGAACTGTAGCAGCTTGAGAATGATTGATTTTTCTCTAAATTCTTTGTCTGGAACTATACCCTTGTCGTTAGGAGGGTTGTCGAATCTTGAGGAGTTTATGATTAGTGACAACAATGTCACCGGTTCGATCCCTTCAAACCTTTCTAATTTGACGAACCTGATGCAGCTGCAGCTCGACACCAATCAGATCTCCGGTTTGATTCCACCGGAGATAGGGATGTTGTCGATGCTGATTGTTTTTTTCGCTTGGCAAAACCAGCTTGAAGGTAGCATTCCTTCAACTCTGGCTAGTTGTAGCAGTCTCCAAGCACTAGACTTGTCACACAATTCCCTCACGGGTAGCATTCCTGCCGGCTTGTTTCAGCTCAAAAACCTCACAAAGGTTCTTTTGATTTCGAATGACATCTCGGGTTTCATTCCACCAAGTATTGGTAACTGCAGCTCTCTGTTTCGGTTGCGGCTTGGGAACAACAGGATTGCTGGTGGGATTCCGAAAGCAATTGGAGACCTTAGGAGCTTAAACTTTCTTGACCTGTCCGGCAACCGCCTTTCTGGGCAAGTGCCTGATGAGATAGGGAGCTGCACAGAGCTGCAAATGATAGACTTGAGCAACAATACTTTGGAAGGCCCCCTGCCTAACACATTGTCATCACTATCAGGACTTCAAGTCTTGGATGTTTCAGTTAATCAGTTTTCGGAGCAGATACCAGCGAGCTTGGCTCGTCTTGTTTCGTTAAACAAGCTTATTCTGAGCAGGAACTTGTTCTCTGGATCGATACCAGCATCGCTTGGCCTGTGTTCAAGTCTCCAATTGCTTGATCTTAGTAGCAACAAGCTCACTGGCTCAATCCCCGTGGAGCTTGGGAGGATCGAAGCCCTTGAAATCGCATTAAATTTGAGCTGCAATGCACTCTCAGGCCCCATCCCACCTCAAATATCAGCACTCACCAAGCTTTCGATACTAGACCTTTCACATAACCAGCTTGATGGGGACTTGAGTCCACTTGCAGGGCTCGATAATCTTGTCTCTCTTAATGTGTCGCATAACAAACTCGGCGGCTATCTTCCAGACAACAAGCTTTTCAGACAGCTGTCACCAATGGATTTGGCTGGAAATGAAGGCCTTTGCTCTTCAAACCGTGACTCGTGTTTCCTTAGTGATGTTGGCCGCAAAGGACTTACAAGAAATCAAAATGAAACGAGGCGGTCACGCAGGCTTAAGCTAGCAATTGCATTGCTGATCACCCTGACAGTTGCAATGGTGGTTATGGGGATCGTTGCAGTGATTAGAGCGCAAAGAGCTATCAGAGATGATGACGATTCAGAACTGGGGAACTCATTGACTTGGCAGTTCACTCCATTCCAGAAGCTAAATTTCTCGGTTGAGCAAGTGCTAAGATGCCTGGTGGATGTCAATGTAATTGGAAAAGGGTGTTCTGGGGTTGTGTACCGCGCTGATATGGACAATGGGGAAGTCATTGCAGTGAAGAAGCTCTGGCCAACAACAGTTGCCGCAGACAATGGATGTTGTAATGATGAAAAATGTGGAGTTCGTGATTCATTCTCAGCAGAGGTCAAAACACTTGGCTCAATCCGGCACAAGAACATTGTTCGATTCTTGGGTTGTTGCTGGAATAAGAACACAAGATTGCTCATGTATGATTACATGCCTAATGGAAGCCTGGGAAGTATTCTGCATGAGAGGACAGGGCATGCCTTCGAATGGGAAGTTAGGTACCAAATTTTGTTGGGTGCAGCTCAAGGCATTGCCTATTTGCACCATGATTGTGTTCCTCCAATTGTTCACAGAGATATCAAGGCCAATAACATCCTCATTGGCCTTGAGTTTGAACCTTACATTGCTGATTTTGGCCTAGCCAAACTTGTTGATGATGGCGATTTCGCTCGGTCCTCCAACACAGTTGCTGGTTCCTATGGCTATATTGCTCCTGAATATGGATACATGATGAAGATCACAGAGAAGAGCGATGTTTATAGCTATGGAGTTGTTGTATTGGAAGTCTTGACAGGGAAGCAACCAATAGATCCAACAATACCAGAGGGGCTACATGTAGTGGATTGGGTAAGACGGAAGAGGGGAAGCGTTGAAGTCCTTGACCCAAGCCTCCTATCGAGACCCGAATCGGAGATAGAGGAAATGATGCAGGCATTAGGCATAGCCTTGTTGTGTGTAAACTCAACCCCTGATGAAAGACCAACAATGAAAGATGTGGCAGCAATGCTAAAAGAAATCAAACATGAGAGGGAGGAGTATGCCAAGGTTGATATGCTCCTCAAAGGATCACCGGCGAATGACTCGCGCGAAAATAAGAACCCTAATGGAGTTTTGGCAACCTCATCATCAGCAGCAGCAATGAAAAGCTTGTTTGCAAAAAGCAACGACACAAGTTTTTCTGCATCCACATTGCTTTACTCATCTTCCTCCTCTAGTGCCAATAAAATGGGTTTCAAGTGA

Protein Analysis

1148

Amino Acids

123.92

Weight (kDa)

5.52

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 109 - 169 1.4e-08 Leucine rich repeat
LRR_14 PF23598 225 - 361 4.8e-13 Leucine-rich repeat region
LRR_8 PF13855 447 - 506 1.3e-06 Leucine rich repeat
LRR_14 PF23598 468 - 692 8e-14 Leucine-rich repeat region
Pkinase PF00069 794 - 1068 3.4e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 795 - 1070 1.4e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24240
fragaria_vesca FvH4_4g05730
malus_domestica MD00G1164700.v1.1 MD16G1236300.v1.1
prunus_persica Prupe.1G060700_v2.0.a1
pyrus_communis pycom13g20340
rosa_chinensis RchiOBHm_Chr4g0396901
rosa_laevigata RLG00000009492
rosa_multiflora Rmu_sc0008929.1_g000009
rosa_roxburghii Rroxscaffold_5G00341810
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4BG071300 Rh4CG079500 Rh4DG068900
rosa_wichuraiana Rw4G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 545
AasI GACNNNNNNGTC 1 cut(s) 1082
AatII GACGTC 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 545
Acc65I GGTACC 1 cut(s) 2703
AccB1I GGYRCC 1 cut(s) 2703
AccB7I CCANNNNNTGG 1 cut(s) 1397
AccII CGCG 4 cut(s) 886, 2421, 3289, 3291
AclWI GGATC 8 cut(s) 583, 874, 1090, 1747, 1844, 2249, 3008, 3277
AcoI YGGCCR 2 cut(s) 2134, 2465
AcsI RAATTY 5 cut(s) 493, 1006, 1861, 2341, 2709
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 3 cut(s) 650, 2417, 2705
AflII CTTAAG 2 cut(s) 902, 2186
AflIII ACRYGT 1 cut(s) 3037
AgeI ACCGGT 1 cut(s) 1088
AhdI GACNNNNNGTC 1 cut(s) 1634
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 3 cut(s) 149, 2370, 2649
AloI GAACNNNNNNTCC 2 cut(s) 285, 317
Alw21I GWGCWC 3 cut(s) 536, 603, 678
Alw26I GTCTC 6 cut(s) 83, 1274, 1467, 1778, 1999, 3097
AlwI GGATC 8 cut(s) 583, 874, 1090, 1747, 1844, 2249, 3008, 3277
AlwNI CAGNNNCTG 2 cut(s) 922, 2894
Ama87I CYCGRG 4 cut(s) 25, 602, 893, 1375
ApoI RAATTY 5 cut(s) 493, 1006, 1861, 2341, 2709
AsiGI ACCGGT 1 cut(s) 1088
Asp700I GAANNNNTTC 1 cut(s) 1730
Asp718I GGTACC 1 cut(s) 2703
AspLEI GCGC 4 cut(s) 356, 2263, 2423, 3291
AspS9I GGNCC 4 cut(s) 228, 1595, 1886, 2877
AsuC2I CCSGG 2 cut(s) 583, 668
AsuII TTCGAA 2 cut(s) 1364, 2688
AsuNHI GCTAGC 1 cut(s) 2191
AvaI CYCGRG 4 cut(s) 25, 602, 893, 1375
AvaII GGWCC 2 cut(s) 228, 2877
AxyI CCTNAGG 1 cut(s) 1478
BalI TGGCCA 1 cut(s) 2467
BanI GGYRCC 1 cut(s) 2703
BanII GRGCYC 3 cut(s) 603, 678, 1983
BarI GAAGNNNNNNTAC 2 cut(s) 1228, 1260
BauI CACGAG 1 cut(s) 2113
Bbv12I GWGCWC 3 cut(s) 536, 603, 678
BbvCI CCTCAGC 2 cut(s) 115, 419
BccI CCATC 5 cut(s) 380, 457, 1898, 1949, 2855
BcgI CGANNNNNNTGC 2 cut(s) 1111, 1145
BciT130I CCWGG 3 cut(s) 151, 2372, 2651
BciVI GTATCC 1 cut(s) 2921
BclI TGATCA 1 cut(s) 2208
BcnI CCSGG 2 cut(s) 583, 668
BcoDI GTCTC 6 cut(s) 83, 1274, 1467, 1778, 1999, 3097
BfaI CTAG 7 cut(s) 290, 1257, 1280, 1931, 2192, 2843, 3417
BfmI CTRYAG 5 cut(s) 685, 911, 973, 1136, 1405
BfoI RGCGCY 1 cut(s) 357
BfrI CTTAAG 2 cut(s) 902, 2186
BfuAI ACCTGC 1 cut(s) 545
BfuI GTATCC 1 cut(s) 2921
BglII AGATCT 1 cut(s) 1155
BlpI GCTNAGC 2 cut(s) 530, 758
Bme1390I CCNGG 5 cut(s) 151, 583, 668, 2372, 2651
Bme18I GGWCC 2 cut(s) 228, 2877
BmeRI GACNNNNNGTC 1 cut(s) 1634
BmeT110I CYCGRG 4 cut(s) 25, 602, 893, 1375
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 4 cut(s) 228, 1595, 1886, 2877
BmiI GGNNCC 8 cut(s) 155, 200, 370, 467, 1597, 1888, 2705, 2899
BmrFI CCNGG 5 cut(s) 151, 583, 668, 2372, 2651
BmrI ACTGGG 3 cut(s) 515, 519, 2306
BmsI GCATC 6 cut(s) 1119, 1185, 1760, 2357, 3120, 3395
BmtI GCTAGC 1 cut(s) 2195
BmuI ACTGGG 3 cut(s) 515, 519, 2306
BoxI GACNNNNGTC 2 cut(s) 1965, 2059
BplI GAGNNNNNCTC 2 cut(s) 1672, 1704
Bpu10I CCTNAGC 2 cut(s) 115, 419
Bpu1102I GCTNAGC 2 cut(s) 530, 758
Bpu14I TTCGAA 2 cut(s) 1364, 2688
BpuEI CTTGAG 7 cut(s) 234, 1003, 1076, 1594, 1984, 2712, 2831
BpuMI CCSGG 2 cut(s) 583, 668
Bsa29I ATCGAT 1 cut(s) 1742
BsaBI GATNNNNATC 1 cut(s) 2939
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 2 cut(s) 1467, 3097
BsaJI CCNNGG 3 cut(s) 1821, 2650, 3246
BsaWI WCCGGW 5 cut(s) 365, 872, 1088, 1160, 1174
BsaXI ACNNNNNCTCC 6 cut(s) 38, 68, 285, 315, 2964, 2994
Bse118I RCCGGY 4 cut(s) 614, 1088, 1319, 3274
Bse1I ACTGG 7 cut(s) 200, 230, 514, 521, 574, 1813, 2301
Bse21I CCTNAGG 1 cut(s) 1478
Bse3DI GCAATG 9 cut(s) 1879, 2201, 2232, 2445, 2733, 2826, 3213, 3348, 3392
Bse8I GATNNNNATC 1 cut(s) 2939
BseBI CCWGG 3 cut(s) 151, 2372, 2651
BseCI ATCGAT 1 cut(s) 1742
BseDI CCNNGG 3 cut(s) 1821, 2650, 3246
BseGI GGATG 8 cut(s) 372, 1191, 1648, 1890, 2383, 2498, 2796, 3386
BseJI GATNNNNATC 1 cut(s) 2939
BseMI GCAATG 9 cut(s) 1879, 2201, 2232, 2445, 2733, 2826, 3213, 3348, 3392
BseMII CTCAG 9 cut(s) 129, 390, 433, 521, 620, 825, 1709, 1896, 2547
BseNI ACTGG 7 cut(s) 200, 230, 514, 521, 574, 1813, 2301
BseRI GAGGAG 7 cut(s) 43, 151, 194, 1073, 3251, 3251, 3403
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 3 cut(s) 379, 1534, 2742
Bsh1236I CGCG 4 cut(s) 886, 2421, 3289, 3291
BshNI GGYRCC 1 cut(s) 2703
BshTI ACCGGT 1 cut(s) 1088
BshVI ATCGAT 1 cut(s) 1742
BsiHKAI GWGCWC 3 cut(s) 536, 603, 678
BsiHKCI CYCGRG 4 cut(s) 25, 602, 893, 1375
BslFI GGGAC 1 cut(s) 1973
BsmAI GTCTC 6 cut(s) 83, 1274, 1467, 1778, 1999, 3097
BsmFI GGGAC 1 cut(s) 1973
BsmI GAATGC 2 cut(s) 1239, 1311
Bso31I GGTCTC 2 cut(s) 1467, 3097
BsoBI CYCGRG 4 cut(s) 25, 602, 893, 1375
Bsp119I TTCGAA 2 cut(s) 1364, 2688
Bsp1286I GDGCHC 4 cut(s) 536, 603, 678, 1983
Bsp1720I GCTNAGC 2 cut(s) 530, 758
BspCNI CTCAG 9 cut(s) 128, 391, 432, 522, 621, 826, 1710, 1895, 2546
BspDI ATCGAT 1 cut(s) 1742
BspFNI CGCG 4 cut(s) 886, 2421, 3289, 3291
BspLI GGNNCC 8 cut(s) 155, 200, 370, 467, 1597, 1888, 2705, 2899
BspMAI CTGCAG 4 cut(s) 689, 915, 1140, 1409
BspMI ACCTGC 1 cut(s) 545
BspOI GCTAGC 1 cut(s) 2195
BspPI GGATC 8 cut(s) 583, 874, 1090, 1747, 1844, 2249, 3008, 3277
BspQI GCTCTTC 3 cut(s) 666, 2104, 2945
BspT104I TTCGAA 2 cut(s) 1364, 2688
BspT107I GGYRCC 1 cut(s) 2703
BspTI CTTAAG 2 cut(s) 902, 2186
BspTNI GGTCTC 2 cut(s) 1467, 3097
BsrDI GCAATG 9 cut(s) 1879, 2201, 2232, 2445, 2733, 2826, 3213, 3348, 3392
BsrFI RCCGGY 4 cut(s) 614, 1088, 1319, 3274
BsrI ACTGG 7 cut(s) 200, 230, 514, 521, 574, 1813, 2301
BssAI RCCGGY 4 cut(s) 614, 1088, 1319, 3274
BssECI CCNNGG 3 cut(s) 1821, 2650, 3246
BssNI GRCGYC 1 cut(s) 384
BssSI CACGAG 1 cut(s) 2113
BssT1I CCWWGG 1 cut(s) 3246
Bst2BI CACGAG 1 cut(s) 2113
Bst2UI CCWGG 3 cut(s) 151, 2372, 2651
Bst4CI ACNGT 6 cut(s) 700, 974, 2108, 2221, 2476, 2890
Bst6I CTCTTC 7 cut(s) 149, 322, 666, 954, 2104, 2945, 3056
BstACI GRCGYC 1 cut(s) 384
BstAFI CTTAAG 2 cut(s) 902, 2186
BstAPI GCANNNNNTGC 2 cut(s) 919, 922
BstBI TTCGAA 2 cut(s) 1364, 2688
BstC8I GCNNGC 7 cut(s) 741, 1321, 1681, 2183, 2193, 2682, 3135
BstDSI CCRYGG 1 cut(s) 1821
BstENI CCTNNNNNAGG 3 cut(s) 460, 819, 1346
BstF5I GGATG 8 cut(s) 372, 1191, 1648, 1890, 2383, 2498, 2796, 3386
BstFNI CGCG 4 cut(s) 886, 2421, 3289, 3291
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 4 cut(s) 356, 2263, 2423, 3291
BstMAI GTCTC 6 cut(s) 83, 1274, 1467, 1778, 1999, 3097
BstMWI GCNNNNNNNGC 9 cut(s) 607, 907, 919, 922, 928, 1138, 1225, 1685, 2267
BstNI CCWGG 3 cut(s) 151, 2372, 2651
BstNSI RCATGY 3 cut(s) 2638, 2684, 3041
BstPAI GACNNNNGTC 2 cut(s) 1965, 2059
BstSCI CCNGG 5 cut(s) 149, 581, 666, 2370, 2649
BstSFI CTRYAG 5 cut(s) 685, 911, 973, 1136, 1405
BstUI CGCG 4 cut(s) 886, 2421, 3289, 3291
BstX2I RGATCY 2 cut(s) 1155, 3013
BstXI CCANNNNNNTGG 4 cut(s) 225, 509, 1955, 3430
BstYI RGATCY 2 cut(s) 1155, 3013
Bsu15I ATCGAT 1 cut(s) 1742
Bsu36I CCTNAGG 1 cut(s) 1478
BsuI GTATCC 1 cut(s) 2921
BsuTUI ATCGAT 1 cut(s) 1742
BtgI CCRYGG 1 cut(s) 1821
BtgZI GCGATG 2 cut(s) 1737, 2970
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 8 cut(s) 372, 1191, 1648, 1890, 2383, 2498, 2796, 3386
BtsI GCAGTG 2 cut(s) 2256, 2457
BtsIMutI CAGTG 4 cut(s) 396, 1806, 2256, 2457
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 7 cut(s) 741, 1321, 1681, 2183, 2193, 2682, 3135
CaiI CAGNNNCTG 2 cut(s) 922, 2894
CfoI GCGC 4 cut(s) 356, 2263, 2423, 3291
Cfr10I RCCGGY 4 cut(s) 614, 1088, 1319, 3274
Cfr13I GGNCC 4 cut(s) 228, 1595, 1886, 2877
ClaI ATCGAT 1 cut(s) 1742
Csp6I GTAC 3 cut(s) 649, 2416, 2704
CspAI ACCGGT 1 cut(s) 1088
CspCI CAANNNNNGTGG 2 cut(s) 1803, 1838
CviQI GTAC 3 cut(s) 649, 2416, 2704
DrdI GACNNNNNNGTC 1 cut(s) 1082
DriI GACNNNNNGTC 1 cut(s) 1634
DseDI GACNNNNNNGTC 1 cut(s) 1082
EaeI YGGCCR 2 cut(s) 2134, 2465
Eam1104I CTCTTC 7 cut(s) 149, 322, 666, 954, 2104, 2945, 3056
Eam1105I GACNNNNNGTC 1 cut(s) 1634
EarI CTCTTC 7 cut(s) 149, 322, 666, 954, 2104, 2945, 3056
EciI GGCGGA 1 cut(s) 220
Ecl136II GAGCTC 2 cut(s) 601, 676
Eco130I CCWWGG 1 cut(s) 3246
Eco147I AGGCCT 1 cut(s) 2091
Eco24I GRGCYC 3 cut(s) 603, 678, 1983
Eco31I GGTCTC 2 cut(s) 1467, 3097
Eco32I GATATC 1 cut(s) 2782
Eco47I GGWCC 2 cut(s) 228, 2877
Eco53kI GAGCTC 2 cut(s) 601, 676
Eco81I CCTNAGG 1 cut(s) 1478
Eco88I CYCGRG 4 cut(s) 25, 602, 893, 1375
EcoICRI GAGCTC 2 cut(s) 601, 676
EcoNI CCTNNNNNAGG 3 cut(s) 460, 819, 1346
EcoO109I RGGNCCY 2 cut(s) 1595, 1886
EcoRI GAATTC 1 cut(s) 493
EcoRII CCWGG 3 cut(s) 149, 2370, 2649
EcoRV GATATC 1 cut(s) 2782
EcoT14I CCWWGG 1 cut(s) 3246
EcoT38I GRGCYC 3 cut(s) 603, 678, 1983
ErhI CCWWGG 1 cut(s) 3246
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 1 cut(s) 1973
FauI CCCGC 3 cut(s) 112, 600, 891
FbaI TGATCA 1 cut(s) 2208
FokI GGATG 8 cut(s) 359, 1198, 1655, 1877, 2390, 2505, 2783, 3373
FriOI GRGCYC 3 cut(s) 603, 678, 1983
FspBI CTAG 7 cut(s) 290, 1257, 1280, 1931, 2192, 2843, 3417
GlaI GCGC 4 cut(s) 355, 2262, 2422, 3290
GsaI CCCAGC 1 cut(s) 24
HaeII RGCGCY 1 cut(s) 357
HhaI GCGC 4 cut(s) 356, 2263, 2423, 3291
Hin1I GRCGYC 1 cut(s) 384
Hin6I GCGC 4 cut(s) 354, 2261, 2421, 3289
HinP1I GCGC 4 cut(s) 354, 2261, 2421, 3289
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 6 cut(s) 755, 899, 1709, 1919, 2045, 3349
HpaI GTTAAC 1 cut(s) 579
Hpy166II GTNNAC 7 cut(s) 6, 579, 1970, 2322, 2416, 2773, 3163
Hpy8I GTNNAC 7 cut(s) 6, 579, 1970, 2322, 2416, 2773, 3163
Hpy99I CGWCG 2 cut(s) 386, 389
HpyCH4III ACNGT 6 cut(s) 700, 974, 2108, 2221, 2476, 2890
HpyCH4IV ACGT 2 cut(s) 258, 384
HpyF10VI GCNNNNNNNGC 9 cut(s) 607, 907, 919, 922, 928, 1138, 1225, 1685, 2267
HpySE526I ACGT 2 cut(s) 258, 384
Hsp92I GRCGYC 1 cut(s) 384
HspAI GCGC 4 cut(s) 354, 2261, 2421, 3289
KpnI GGTACC 1 cut(s) 2707
KroI GCCGGC 1 cut(s) 1319
KroNI GCCGGC 1 cut(s) 1321
Ksp22I TGATCA 1 cut(s) 2208
KspAI GTTAAC 1 cut(s) 579
LguI GCTCTTC 3 cut(s) 666, 2104, 2945
LmnI GCTCC 9 cut(s) 159, 471, 598, 1482, 1545, 1666, 1825, 2920, 3264
LweI GCATC 6 cut(s) 1119, 1185, 1760, 2357, 3120, 3395
MaeI CTAG 7 cut(s) 290, 1257, 1280, 1931, 2192, 2843, 3417
MaeII ACGT 2 cut(s) 258, 384
MaeIII GTNAC 8 cut(s) 259, 1073, 1084, 1287, 1400, 2061, 2108, 2175
MfeI CAATTG 4 cut(s) 1467, 1778, 2196, 2766
MflI RGATCY 2 cut(s) 1155, 3013
MhlI GDGCHC 4 cut(s) 536, 603, 678, 1983
MlsI TGGCCA 1 cut(s) 2467
MluNI TGGCCA 1 cut(s) 2467
MlyI GAGTC 3 cut(s) 1975, 2105, 3278
MmeI TCCRAC 5 cut(s) 216, 405, 522, 2907, 3041
Mox20I TGGCCA 1 cut(s) 2467
MroNI GCCGGC 1 cut(s) 1319
MroXI GAANNNNTTC 1 cut(s) 1730
MscI TGGCCA 1 cut(s) 2467
MseI TTAA 8 cut(s) 578, 903, 1487, 1653, 1703, 1859, 2001, 2187
MslI CAYNNNNRTG 4 cut(s) 63, 791, 2450, 2754
Msp20I TGGCCA 1 cut(s) 2467
MspA1I CMGCKG 2 cut(s) 1135, 2059
MspCI CTTAAG 2 cut(s) 902, 2186
MspR9I CCNGG 5 cut(s) 151, 583, 668, 2372, 2651
MunI CAATTG 4 cut(s) 1467, 1778, 2196, 2766
Mva1269I GAATGC 2 cut(s) 1239, 1311
MvaI CCWGG 3 cut(s) 151, 2372, 2651
MvnI CGCG 4 cut(s) 886, 2421, 3289, 3291
MwoI GCNNNNNNNGC 9 cut(s) 607, 907, 919, 922, 928, 1138, 1225, 1685, 2267
NaeI GCCGGC 1 cut(s) 1321
NciI CCSGG 2 cut(s) 583, 668
NgoMIV GCCGGC 1 cut(s) 1319
NheI GCTAGC 1 cut(s) 2191
NlaIV GGNNCC 8 cut(s) 155, 200, 370, 467, 1597, 1888, 2705, 2899
NmeAIII GCCGAG 1 cut(s) 2001
NmuCI GTSAC 7 cut(s) 259, 1073, 1084, 1287, 2061, 2108, 2175
NspI RCATGY 3 cut(s) 2638, 2684, 3041
NspV TTCGAA 2 cut(s) 1364, 2688
PaeI GCATGC 1 cut(s) 2684
PaeR7I CTCGAG 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 545
PceI AGGCCT 1 cut(s) 2091
PciI ACATGT 1 cut(s) 3037
PciSI GCTCTTC 3 cut(s) 666, 2104, 2945
PctI GAATGC 2 cut(s) 1239, 1311
PdiI GCCGGC 1 cut(s) 1321
PdmI GAANNNNTTC 1 cut(s) 1730
PfeI GAWTC 7 cut(s) 1051, 1168, 1456, 2288, 2525, 2583, 3110
PflMI CCANNNNNTGG 1 cut(s) 1397
PfoI TCCNGGA 1 cut(s) 666
PinAI ACCGGT 1 cut(s) 1088
PleI GAGTC 3 cut(s) 1974, 2105, 3278
PpsI GAGTC 3 cut(s) 1974, 2105, 3278
PscI ACATGT 1 cut(s) 3037
PshAI GACNNNNGTC 2 cut(s) 1965, 2059
Psp124BI GAGCTC 2 cut(s) 603, 678
Psp6I CCWGG 3 cut(s) 149, 2370, 2649
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 3 cut(s) 149, 2370, 2649
PspN4I GGNNCC 8 cut(s) 155, 200, 370, 467, 1597, 1888, 2705, 2899
PspPI GGNCC 4 cut(s) 228, 1595, 1886, 2877
PspXI VCTCGAGB 1 cut(s) 25
PstI CTGCAG 4 cut(s) 689, 915, 1140, 1409
PstNI CAGNNNCTG 2 cut(s) 922, 2894
PsuI RGATCY 2 cut(s) 1155, 3013
PvuII CAGCTG 2 cut(s) 1135, 2059
RsaI GTAC 3 cut(s) 650, 2417, 2705
RsaNI GTAC 3 cut(s) 649, 2416, 2704
RseI CAYNNNNRTG 4 cut(s) 63, 791, 2450, 2754
SacI GAGCTC 2 cut(s) 603, 678
SapI GCTCTTC 3 cut(s) 666, 2104, 2945
SaqAI TTAA 8 cut(s) 578, 903, 1487, 1653, 1703, 1859, 2001, 2187
Sau96I GGNCC 4 cut(s) 228, 1595, 1886, 2877
SchI GAGTC 3 cut(s) 1975, 2105, 3278
ScrFI CCNGG 5 cut(s) 151, 583, 668, 2372, 2651
SduI GDGCHC 4 cut(s) 536, 603, 678, 1983
SfaNI GCATC 6 cut(s) 1119, 1185, 1760, 2357, 3120, 3395
SfcI CTRYAG 5 cut(s) 685, 911, 973, 1136, 1405
Sfr274I CTCGAG 1 cut(s) 25
SfuI TTCGAA 2 cut(s) 1364, 2688
SgrAI CRCCGGYG 1 cut(s) 3274
SinI GGWCC 2 cut(s) 228, 2877
SlaI CTCGAG 1 cut(s) 25
SmiMI CAYNNNNRTG 4 cut(s) 63, 791, 2450, 2754
SphI GCATGC 1 cut(s) 2684
SseBI AGGCCT 1 cut(s) 2091
SspMI CTAG 7 cut(s) 290, 1257, 1280, 1931, 2192, 2843, 3417
SstI GAGCTC 2 cut(s) 603, 678
StuI AGGCCT 1 cut(s) 2091
StyD4I CCNGG 5 cut(s) 149, 581, 666, 2370, 2649
StyI CCWWGG 1 cut(s) 3246
TaaI ACNGT 6 cut(s) 700, 974, 2108, 2221, 2476, 2890
TaiI ACGT 2 cut(s) 261, 387
TaqII GACCGA 1 cut(s) 2865
TatI WGTACW 1 cut(s) 648
TauI GCSGC 4 cut(s) 1429, 2028, 2139, 2483
TfiI GAWTC 7 cut(s) 1051, 1168, 1456, 2288, 2525, 2583, 3110
Tru1I TTAA 8 cut(s) 578, 903, 1487, 1653, 1703, 1859, 2001, 2187
Tru9I TTAA 8 cut(s) 578, 903, 1487, 1653, 1703, 1859, 2001, 2187
TscAI CASTG 4 cut(s) 403, 1813, 2256, 2457
TseFI GTSAC 7 cut(s) 259, 1073, 1084, 1287, 2061, 2108, 2175
Tsp45I GTSAC 7 cut(s) 259, 1073, 1084, 1287, 2061, 2108, 2175
TspDTI ATGAA 9 cut(s) 1372, 2100, 2178, 2517, 2520, 2951, 3192, 3206, 3359
TspGWI ACGGA 1 cut(s) 3073
TspRI CASTG 4 cut(s) 403, 1813, 2256, 2457
Van91I CCANNNNNTGG 1 cut(s) 1397
Vha464I CTTAAG 2 cut(s) 902, 2186
VpaK11BI GGWCC 2 cut(s) 228, 2877
XagI CCTNNNNNAGG 3 cut(s) 460, 819, 1346
XapI RAATTY 5 cut(s) 493, 1006, 1861, 2341, 2709
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 3 cut(s) 2638, 2684, 3041
XhoI CTCGAG 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 1730
XspI CTAG 7 cut(s) 290, 1257, 1280, 1931, 2192, 2843, 3417
ZraI GACGTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.