Rroxscaffold_5G00341810
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015802
Physical Location & Seq
Reverse (-)
10429632 .. 10433195
3564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00341810.1

Sequence Viewer

Length: 3171 bp
ATGTCGTCGGTTTCATCGATACCCAGCTCGAGGCAATCCTTCTCTTCTTCTTGTTCTTCAATAATCTCCATTATCTATCTATTTCTCATTTCGTCGGCCTTTGCCTCAAACCATGAAGCCTCTCTTCTCTTCTCTTGGCTGCATTCCTCTACTTCTTTCTCTTATTCTTCCTTTTCGAACTGGAACATTCTCGACCCCAATCCATGTAACTGGTCCTCCATAACATGTTCTCCAAATGGTTTTGTCACGGAAATCACTATCCAGTCCATCCCTCTTGAGCTTCCAATCCCATCTAATCTTTCCTCCTTCACCTCCCTTCAAAAACTCGTCATTTCCGGCGGTAATCTCACCGGAAAAATCCCCTCCGACATCGGTGACTGCACTGAGCTACAAGTCATTGACCTAAGCTCAAACTCACTCGTGGGTTCGATCCCTTCATCCATTGGAAGACTTCAAAACCTCCAAGACTTGATTCTCAACTCAAACCAGCTCAGTGGGAAAATCCCGGTTGAGTTTAGCGATTGCATTGGCCTAAAGAATCTTGTCATTTTCGATAATCAGTTTTCCGGGAATATCCCCCCGGAGCTCGGAAAACTGGCCGGTCTTGAAGTGCTGAGAGCAGGAGGGAACAAAAACATTGGTGGGAGAATCCCTGATGAGCTTGGAGACTGCACCAACTTGACTGTTTTGGGCTTGGCTGATACTCAAATTTCTGGTTCTTTGCCGGCCTCGTTGGGTAAGCTTAGCAAGCTTCAGACGCTGTCTATATACACCACAATGATCTCCGGTGAGATTCCTGCTGAGATAGGTAACTGTTCTGAGCTTGTGAACTTGTTTCTTTATGAAAACAGTCTATCCGGTTCGATTCCACCGCAGCTCGGTAGGCTTAAGAAGCTTGAGCAGTTGTTGTTGTGGCAGAATAGTCTTGTTGGGGTGATCCCTGAAGAGATTGGGAAATGTAGTAGCTTGAAAATGATTGATTTTTCATTAAATTCTTTGTCTGGTACTATTCCATTGTCTCTAGGAGGGCTGTCAAACCTTGAGGAGTTTATGATTAGTAACAACAATGTCACCGGTTCAATACCTTCCAGTCTTTGCAATGCAACGAATTTGATTCAGTTGCAGCTTGATACGAATCAGATCTCCGGTTTGATTCCACCGGAGATTGGGAAGTTGTCAAAGCTGACTGTGTTCTTTGCTTGGCAAAACCAGCTTGAAGGAAGCATTCCTTCATCTTTGTCTAGTTTGAGTAATCTTCAAGCTCTAGATTTGTCACACAATTCACTCACTGGTAGCATACCTTCTGGCTTGTTTCAGCTGCAAAACCTCACAAAGATTCTCTTGATTTCTAACGATATTTCGGGCTCAATCCCACCCGAAATTGGTAACTGCAGCTCTCTTGTAAGGCTGAGGCTTGGAAATAACAGGATTACTGGTGGGATTCCTAGAGGCATTGGAAATCTCAGGAGCTTAAATTTTCTTGATCTTTCAGGGAATCGACTTTCTGGGTCAATTCCTGATGAGATTGGGAGTTGCACAGAGCTACAAATGATAGACCTCAGCAACAATACTTTAGAGGGTCCCCTGCCTAATTCATTGTCATCACTCTCAGGACTTCAAGTCTTGGATGTCTCCATCAATCAATTTTCGAGCCAGATACCTGCAAGCTTGGCTCGCCTTGTTACGTTGAACACGCTGATTCTGAGCAGGAACTCATTCTCTGGATTAATACCTGCATCACTCGGCCTATGTTCGAGTCTGCAATTGCTTGATCTTAGCAGCAACAAGCTCACTGGCAAAATACCTGTGGAGCTTGGCAAAATTGAATCCCTTGAAATTACTCTAAATTTGAGCTGCAACGGACTCTCTGGATCAATCCCACCTCAAATATCAGCACTCAACAAGCTTTTCGTACTAGACCTTTCACATAACCAGCTTGAAGGGGATTTGAGTCCACTCTCTTCTGGCCTTAACACTCTTGATTCTCTAAATGTGTCTTACAATAACCTCGTTGGCTATCTTCCAGACAACAAGCTTTTCAGACAACTGTCTCCAATGGATTTAGCCGGAAATGAACGCCTTTGTTCTTCAAACCGAGACTCGTGTTTCTTGAGTGATGTTGGCAGGCCAGGACTATCAAGGAACCAAAATGACGTAAGGCGGTCAAGGAGGCTTAAGCTGGCAATTGCATTGCTGATCACCTTGACAGTTGCAATGGTTGTTATGGGGATAATTGCAGTGATTAGAGCACGAAGGACTATTCGGGATGATGATGATTCAGAGTTGGGGAGCTCATGGGCATGGCAATTCACTCCATTCCAGAAGCTAAATTTCTCGGTTGACCAAGTGCTTAAGAGCCTAGTGGATGCCAATGTGATTGGAAAAGGGTGTTCTGGGGTTGTTTATCGTGCTGATATGGACAATGGTGAAGTCATTGCAGTGAAGAAGCTCTGGCCAACTACAGTTGCTGCAGACAATGGATGTTGCAATGATGAAAAATGTGGAGTTCGTGATTCGTTCTCAGCAGAGGTCAAAACACTTGGCTCAATCCGTCACAAGAACATTGTTAGGTTCTTGGGATGTTGCTGGAATAGGAACACAAGGCTGCTCATGTATGACTATATGCCTAATGGAAGCTTGGGCAGTATTCTCCATGAGAGGACAGGACATGCCTTTGAGTGGGAGCTTAGCTATGGTGTAGTTGTATTGGAAGTTTTAACAGGGAAGCAACCAATAGATCCAACAATACCAGATGGGCTACATGTAGTGGATTGGGTGAGACAGAAGAGAGGAAATGTTGAAGTGCTTGACCCTATCCTACTATCAAGACCAGAAACAGAAATAGAGGAAATGATGCAAGCATTAGGCATAGCCCTGTTATGTGTAAACTCCTCTCCAGTTGAAAGGCCAACAATGAAAGATGTAGCTGCAATGCTCAAGGAAATCAGGCATGAAAGGGAAGAGTATGCTAAGGTCGATGTGCTTCTCAAAGGGTCTCCGGCGAATGATGCTGCTCGAGAAAATAAGAACTCTGCTGGAGTTTTGGAAACATCATCATCATCGGCAGCAGCGGCAAAAAGGTTGTATGCTAAAAGCAACAACACAAGCTTTTCTGCATCCACTCTTCTTTACTCATCTTCTTCCTCTAGTGCAAAAATGGGTTTCAAGTGA

Protein Analysis

1056

Amino Acids

113.61

Weight (kDa)

5.45

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 102 - 207 3.4e-07 Leucine-rich repeat region
LRR_8 PF13855 105 - 164 4.9e-06 Leucine rich repeat
LRR_8 PF13855 129 - 188 4.5e-06 Leucine rich repeat
LRR_14 PF23598 184 - 306 4.8e-11 Leucine-rich repeat region
LRR_14 PF23598 310 - 470 5.4e-09 Leucine-rich repeat region
LRR_14 PF23598 432 - 589 7.5e-10 Leucine-rich repeat region
LRR_14 PF23598 508 - 612 8.3e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 791 - 888 1.6e-09 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 791 - 887 6e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24240
fragaria_vesca FvH4_4g05730
malus_domestica MD00G1164700.v1.1 MD16G1236300.v1.1
prunus_persica Prupe.1G060700_v2.0.a1
pyrus_communis pycom13g20340
rosa_chinensis RchiOBHm_Chr4g0396901
rosa_laevigata RLG00000009492
rosa_multiflora Rmu_sc0008929.1_g000009
rosa_roxburghii Rroxscaffold_5G00341810
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4BG071300 Rh4CG079500 Rh4DG068900
rosa_wichuraiana Rw4G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1669, 1741
AciI CCGC 4 cut(s) 339, 872, 2161, 3071
AclWI GGATC 4 cut(s) 424, 931, 1879, 2732
AcoI YGGCCR 2 cut(s) 597, 2453
AcsI RAATTY 6 cut(s) 708, 991, 1108, 1474, 1846, 2329
AcuI CTGAAG 2 cut(s) 737, 963
AfaI GTAC 2 cut(s) 1006, 1914
AfiI CCNNNNNNNGG 6 cut(s) 30, 587, 878, 1166, 1382, 2679
AflII CTTAAG 3 cut(s) 887, 2174, 2351
AflIII ACRYGT 2 cut(s) 224, 2761
AgeI ACCGGT 1 cut(s) 1073
AhdI GACNNNNNGTC 1 cut(s) 1619
AjnI CCWGG 1 cut(s) 2128
AjuI GAANNNNNNNTTGG 2 cut(s) 456, 488
Alw21I GWGCWC 3 cut(s) 588, 2251, 2294
Alw26I GTCTC 7 cut(s) 660, 1023, 1636, 2055, 2091, 2773, 3000
AlwI GGATC 4 cut(s) 424, 931, 1879, 2732
AlwNI CAGNNNCTG 2 cut(s) 760, 2468
Ama87I CYCGRG 2 cut(s) 28, 3015
AoxI GGCC 9 cut(s) 96, 529, 597, 726, 1744, 1966, 2126, 2453, 2906
ApoI RAATTY 6 cut(s) 708, 991, 1108, 1474, 1846, 2329
AseI ATTAAT 1 cut(s) 1727
AsiGI ACCGGT 1 cut(s) 1073
Asp700I GAANNNNTTC 1 cut(s) 1715
AspS9I GGNCC 2 cut(s) 213, 1580
AsuC2I CCSGG 3 cut(s) 506, 568, 581
AsuHPI GGTGA 9 cut(s) 301, 340, 386, 800, 946, 1063, 2191, 2438, 2788
AsuII TTCGAA 1 cut(s) 176
AvaI CYCGRG 2 cut(s) 28, 3015
AvaII GGWCC 2 cut(s) 213, 1580
BalI TGGCCA 1 cut(s) 2455
BanII GRGCYC 3 cut(s) 588, 1367, 2294
BarI GAAGNNNNNNTAC 2 cut(s) 1285, 1317
BauI CACGAG 2 cut(s) 419, 2101
BbsI GAAGAC 1 cut(s) 454
Bbv12I GWGCWC 3 cut(s) 588, 2251, 2294
BbvCI CCTCAGC 2 cut(s) 1409, 1559
BccI CCATC 4 cut(s) 275, 298, 1643, 2747
BciT130I CCWGG 1 cut(s) 2130
BclI TGATCA 1 cut(s) 2196
BcnI CCSGG 3 cut(s) 506, 568, 581
BcoDI GTCTC 7 cut(s) 660, 1023, 1636, 2055, 2091, 2773, 3000
BfaI CTAG 7 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 3147
BfmI CTRYAG 3 cut(s) 1390, 2460, 2469
BfrI CTTAAG 3 cut(s) 887, 2174, 2351
BfuAI ACCTGC 2 cut(s) 1669, 1741
BglII AGATCT 1 cut(s) 1140
BlpI GCTNAGC 2 cut(s) 743, 2687
Bme1390I CCNGG 4 cut(s) 506, 568, 581, 2130
Bme18I GGWCC 2 cut(s) 213, 1580
BmeRI GACNNNNNGTC 1 cut(s) 1619
BmeT110I CYCGRG 2 cut(s) 28, 3015
BmgT120I GGNCC 2 cut(s) 213, 1580
BmiI GGNNCC 3 cut(s) 1581, 1582, 2144
BmrFI CCNGG 4 cut(s) 506, 568, 581, 2130
BmsI GCATC 5 cut(s) 1745, 2356, 2844, 2998, 3125
BoxI GACNNNNGTC 1 cut(s) 2047
BpiI GAAGAC 1 cut(s) 454
BpmI CTGGAG 2 cut(s) 2880, 3057
Bpu10I CCTNAGC 4 cut(s) 404, 1409, 1559, 2970
Bpu1102I GCTNAGC 2 cut(s) 743, 2687
Bpu14I TTCGAA 1 cut(s) 176
BpuEI CTTGAG 5 cut(s) 296, 917, 1061, 2131, 2921
BpuMI CCSGG 3 cut(s) 506, 568, 581
Bsa29I ATCGAT 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 1134
BsaI GGTCTC 1 cut(s) 3000
BsaJI CCNNGG 1 cut(s) 579
BsaWI WCCGGW 6 cut(s) 350, 785, 857, 1073, 1145, 1159
BsaXI ACNNNNNCTCC 4 cut(s) 200, 214, 230, 244
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 587, 878, 1166, 1382, 2679
Bse118I RCCGGY 3 cut(s) 599, 724, 1073
Bse1I ACTGG 9 cut(s) 185, 215, 262, 600, 1089, 1294, 1438, 1798, 2897
Bse3DI GCAATG 6 cut(s) 1105, 2189, 2220, 2433, 2494, 2937
Bse8I GATNNNNATC 1 cut(s) 1134
BseBI CCWGG 1 cut(s) 2130
BseCI ATCGAT 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 8 cut(s) 267, 437, 1633, 2272, 2371, 2486, 2585, 3116
BseJI GATNNNNATC 1 cut(s) 1134
BseLI CCNNNNNNNGG 6 cut(s) 30, 587, 878, 1166, 1382, 2679
BseMI GCAATG 6 cut(s) 1105, 2189, 2220, 2433, 2494, 2937
BseNI ACTGG 9 cut(s) 185, 215, 262, 600, 1089, 1294, 1438, 1798, 2897
BseRI GAGGAG 2 cut(s) 1058, 2881
BseYI CCCAGC 1 cut(s) 23
BsgI GTGCAG 2 cut(s) 364, 655
BshFI GGCC 9 cut(s) 98, 531, 599, 728, 1746, 1968, 2128, 2455, 2908
BshTI ACCGGT 1 cut(s) 1073
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 3 cut(s) 588, 2251, 2294
BsiHKCI CYCGRG 2 cut(s) 28, 3015
BslFI GGGAC 1 cut(s) 1566
BslI CCNNNNNNNGG 6 cut(s) 30, 587, 878, 1166, 1382, 2679
BsmAI GTCTC 7 cut(s) 660, 1023, 1636, 2055, 2091, 2773, 3000
BsmFI GGGAC 1 cut(s) 1566
BsmI GAATGC 2 cut(s) 142, 1224
BsnI GGCC 9 cut(s) 98, 531, 599, 728, 1746, 1968, 2128, 2455, 2908
Bso31I GGTCTC 1 cut(s) 3000
BsoBI CYCGRG 2 cut(s) 28, 3015
Bsp119I TTCGAA 1 cut(s) 176
Bsp1286I GDGCHC 4 cut(s) 588, 1367, 2251, 2294
Bsp143I GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
Bsp1720I GCTNAGC 2 cut(s) 743, 2687
BspACI CCGC 4 cut(s) 339, 872, 2161, 3071
BspANI GGCC 9 cut(s) 98, 531, 599, 728, 1746, 1968, 2128, 2455, 2908
BspDI ATCGAT 1 cut(s) 17
BspLI GGNNCC 3 cut(s) 1581, 1582, 2144
BspMAI CTGCAG 2 cut(s) 1394, 2473
BspMI ACCTGC 2 cut(s) 1669, 1741
BspPI GGATC 4 cut(s) 424, 931, 1879, 2732
BspT104I TTCGAA 1 cut(s) 176
BspTI CTTAAG 3 cut(s) 887, 2174, 2351
BspTNI GGTCTC 1 cut(s) 3000
BsrDI GCAATG 6 cut(s) 1105, 2189, 2220, 2433, 2494, 2937
BsrFI RCCGGY 3 cut(s) 599, 724, 1073
BsrI ACTGG 9 cut(s) 185, 215, 262, 600, 1089, 1294, 1438, 1798, 2897
BssAI RCCGGY 3 cut(s) 599, 724, 1073
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
BssSI CACGAG 2 cut(s) 419, 2101
Bst2BI CACGAG 2 cut(s) 419, 2101
Bst2UI CCWGG 1 cut(s) 2130
Bst4CI ACNGT 7 cut(s) 685, 815, 851, 1189, 2049, 2209, 2464
Bst6I CTCTTC 8 cut(s) 49, 129, 134, 939, 1966, 2780, 2955, 3129
BstAFI CTTAAG 3 cut(s) 887, 2174, 2351
BstBI TTCGAA 1 cut(s) 176
BstC8I GCNNGC 7 cut(s) 726, 749, 1666, 1675, 2126, 2181, 2859
BstF5I GGATG 8 cut(s) 267, 437, 1633, 2272, 2371, 2486, 2585, 3116
BstKTI GATC 9 cut(s) 432, 783, 939, 1143, 1486, 1774, 1874, 2199, 2740
BstMAI GTCTC 7 cut(s) 660, 1023, 1636, 2055, 2091, 2773, 3000
BstMBI GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
BstMWI GCNNNNNNNGC 9 cut(s) 748, 757, 883, 892, 1210, 1670, 1674, 3008, 3071
BstNI CCWGG 1 cut(s) 2130
BstNSI RCATGY 3 cut(s) 228, 2672, 2765
BstPAI GACNNNNGTC 1 cut(s) 2047
BstSCI CCNGG 4 cut(s) 504, 566, 579, 2128
BstSFI CTRYAG 3 cut(s) 1390, 2460, 2469
BstV2I GAAGAC 1 cut(s) 454
BstX2I RGATCY 2 cut(s) 1140, 2737
BstXI CCANNNNNNTGG 2 cut(s) 210, 494
BstYI RGATCY 2 cut(s) 1140, 2737
Bsu15I ATCGAT 1 cut(s) 17
BsuRI GGCC 9 cut(s) 98, 531, 599, 728, 1746, 1968, 2128, 2455, 2908
BsuTUI ATCGAT 1 cut(s) 17
BtsCI GGATG 8 cut(s) 267, 437, 1633, 2272, 2371, 2486, 2585, 3116
BtsI GCAGTG 2 cut(s) 2244, 2445
BtsIMutI CAGTG 6 cut(s) 381, 499, 1287, 1791, 2244, 2445
BveI ACCTGC 2 cut(s) 1669, 1741
Cac8I GCNNGC 7 cut(s) 726, 749, 1666, 1675, 2126, 2181, 2859
CaiI CAGNNNCTG 2 cut(s) 760, 2468
Cfr10I RCCGGY 3 cut(s) 599, 724, 1073
Cfr13I GGNCC 2 cut(s) 213, 1580
ClaI ATCGAT 1 cut(s) 17
CseI GACGC 1 cut(s) 766
Csp6I GTAC 2 cut(s) 1005, 1913
CspAI ACCGGT 1 cut(s) 1073
CviQI GTAC 2 cut(s) 1005, 1913
DpnI GATC 9 cut(s) 431, 782, 938, 1142, 1485, 1773, 1873, 2198, 2739
DpnII GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
DriI GACNNNNNGTC 1 cut(s) 1619
EaeI YGGCCR 2 cut(s) 597, 2453
Eam1104I CTCTTC 8 cut(s) 49, 129, 134, 939, 1966, 2780, 2955, 3129
Eam1105I GACNNNNNGTC 1 cut(s) 1619
EarI CTCTTC 8 cut(s) 49, 129, 134, 939, 1966, 2780, 2955, 3129
Ecl136II GAGCTC 2 cut(s) 586, 2292
Eco24I GRGCYC 3 cut(s) 588, 1367, 2294
Eco31I GGTCTC 1 cut(s) 3000
Eco47I GGWCC 2 cut(s) 213, 1580
Eco53kI GAGCTC 2 cut(s) 586, 2292
Eco57I CTGAAG 2 cut(s) 737, 963
Eco88I CYCGRG 2 cut(s) 28, 3015
EcoICRI GAGCTC 2 cut(s) 586, 2292
EcoO109I RGGNCCY 1 cut(s) 1580
EcoRII CCWGG 1 cut(s) 2128
EcoT38I GRGCYC 3 cut(s) 588, 1367, 2294
FalI AAGNNNNNCTT 6 cut(s) 108, 140, 1213, 1245, 1325, 1357
FaqI GGGAC 1 cut(s) 1566
FbaI TGATCA 1 cut(s) 2196
FokI GGATG 8 cut(s) 254, 424, 1640, 2279, 2378, 2493, 2592, 3103
FriOI GRGCYC 3 cut(s) 588, 1367, 2294
FspBI CTAG 7 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 3147
GsaI CCCAGC 1 cut(s) 27
GsuI CTGGAG 2 cut(s) 2880, 3057
HaeIII GGCC 9 cut(s) 98, 531, 599, 728, 1746, 1968, 2128, 2455, 2908
HgaI GACGC 1 cut(s) 766
HincII GTYRAC 1 cut(s) 2341
HindII GTYRAC 1 cut(s) 2341
HindIII AAGCTT 8 cut(s) 740, 749, 893, 1666, 1904, 2033, 2635, 3106
HphI GGTGA 9 cut(s) 301, 340, 386, 800, 946, 1063, 2191, 2438, 2788
Hpy166II GTNNAC 4 cut(s) 829, 1955, 2341, 2887
Hpy188I TCNGA 8 cut(s) 367, 590, 756, 820, 1140, 1704, 2042, 2281
Hpy8I GTNNAC 4 cut(s) 829, 1955, 2341, 2887
Hpy99I CGWCG 2 cut(s) 10, 97
HpyCH4III ACNGT 7 cut(s) 685, 815, 851, 1189, 2049, 2209, 2464
HpyCH4IV ACGT 2 cut(s) 1685, 2154
HpyF10VI GCNNNNNNNGC 9 cut(s) 748, 757, 883, 892, 1210, 1670, 1674, 3008, 3071
HpySE526I ACGT 2 cut(s) 1685, 2154
KflI GGGWCCC 1 cut(s) 1580
KroI GCCGGC 1 cut(s) 724
KroNI GCCGGC 1 cut(s) 726
Ksp22I TGATCA 1 cut(s) 2196
Kzo9I GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
LmnI GCTCC 5 cut(s) 583, 1467, 1810, 2289, 2683
LweI GCATC 5 cut(s) 1745, 2356, 2844, 2998, 3125
MaeI CTAG 7 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 3147
MaeII ACGT 2 cut(s) 1685, 2154
MalI GATC 9 cut(s) 431, 782, 938, 1142, 1485, 1773, 1873, 2198, 2739
MboI GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
MfeI CAATTG 2 cut(s) 1763, 2184
MflI RGATCY 2 cut(s) 1140, 2737
MhlI GDGCHC 4 cut(s) 588, 1367, 2251, 2294
MlsI TGGCCA 1 cut(s) 2455
MluNI TGGCCA 1 cut(s) 2455
MlyI GAGTC 4 cut(s) 1765, 1857, 1960, 2093
MmeI TCCRAC 2 cut(s) 390, 2765
Mox20I TGGCCA 1 cut(s) 2455
MroNI GCCGGC 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 1715
MscI TGGCCA 1 cut(s) 2455
MseI TTAA 8 cut(s) 888, 989, 1472, 1727, 1971, 2175, 2352, 2717
MslI CAYNNNNRTG 3 cut(s) 776, 2299, 2438
Msp20I TGGCCA 1 cut(s) 2455
MspA1I CMGCKG 2 cut(s) 1318, 3071
MspCI CTTAAG 3 cut(s) 887, 2174, 2351
MspR9I CCNGG 4 cut(s) 506, 568, 581, 2130
MunI CAATTG 2 cut(s) 1763, 2184
Mva1269I GAATGC 2 cut(s) 142, 1224
MvaI CCWGG 1 cut(s) 2130
MwoI GCNNNNNNNGC 9 cut(s) 748, 757, 883, 892, 1210, 1670, 1674, 3008, 3071
NaeI GCCGGC 1 cut(s) 726
NciI CCSGG 3 cut(s) 506, 568, 581
NdeII GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
NgoMIV GCCGGC 1 cut(s) 724
NlaIV GGNNCC 3 cut(s) 1581, 1582, 2144
NmeAIII GCCGAG 1 cut(s) 1722
NmuCI GTSAC 5 cut(s) 244, 374, 1069, 1272, 2552
NspI RCATGY 3 cut(s) 228, 2672, 2765
NspV TTCGAA 1 cut(s) 176
PaeR7I CTCGAG 2 cut(s) 28, 3015
PciI ACATGT 2 cut(s) 224, 2761
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 2 cut(s) 142, 1224
PdiI GCCGGC 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 1715
PflFI GACNNNGTC 1 cut(s) 760
PfoI TCCNGGA 1 cut(s) 566
PinAI ACCGGT 1 cut(s) 1073
PleI GAGTC 4 cut(s) 1764, 1857, 1959, 2093
PpsI GAGTC 4 cut(s) 1764, 1857, 1959, 2093
PpuMI RGGWCCY 1 cut(s) 1580
PscI ACATGT 2 cut(s) 224, 2761
PshAI GACNNNNGTC 1 cut(s) 2047
PshBI ATTAAT 1 cut(s) 1727
Psp124BI GAGCTC 2 cut(s) 588, 2294
Psp5II RGGWCCY 1 cut(s) 1580
Psp6I CCWGG 1 cut(s) 2128
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 1 cut(s) 2128
PspN4I GGNNCC 3 cut(s) 1581, 1582, 2144
PspPI GGNCC 2 cut(s) 213, 1580
PspPPI RGGWCCY 1 cut(s) 1580
PspXI VCTCGAGB 1 cut(s) 28
PstI CTGCAG 2 cut(s) 1394, 2473
PstNI CAGNNNCTG 2 cut(s) 760, 2468
PsuI RGATCY 2 cut(s) 1140, 2737
PsyI GACNNNGTC 1 cut(s) 760
PvuII CAGCTG 1 cut(s) 1318
RsaI GTAC 2 cut(s) 1006, 1914
RsaNI GTAC 2 cut(s) 1005, 1913
RseI CAYNNNNRTG 3 cut(s) 776, 2299, 2438
SacI GAGCTC 2 cut(s) 588, 2294
SaqAI TTAA 8 cut(s) 888, 989, 1472, 1727, 1971, 2175, 2352, 2717
Sau3AI GATC 9 cut(s) 429, 780, 936, 1140, 1483, 1771, 1871, 2196, 2737
Sau96I GGNCC 2 cut(s) 213, 1580
SchI GAGTC 4 cut(s) 1765, 1857, 1960, 2093
ScrFI CCNGG 4 cut(s) 506, 568, 581, 2130
SduI GDGCHC 4 cut(s) 588, 1367, 2251, 2294
SfaNI GCATC 5 cut(s) 1745, 2356, 2844, 2998, 3125
SfcI CTRYAG 3 cut(s) 1390, 2460, 2469
Sfr274I CTCGAG 2 cut(s) 28, 3015
SfuI TTCGAA 1 cut(s) 176
SinI GGWCC 2 cut(s) 213, 1580
SlaI CTCGAG 2 cut(s) 28, 3015
SmiMI CAYNNNNRTG 3 cut(s) 776, 2299, 2438
SsiI CCGC 4 cut(s) 339, 872, 2161, 3071
SspMI CTAG 7 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 3147
SstI GAGCTC 2 cut(s) 588, 2294
StyD4I CCNGG 4 cut(s) 504, 566, 579, 2128
TaaI ACNGT 7 cut(s) 685, 815, 851, 1189, 2049, 2209, 2464
TaiI ACGT 2 cut(s) 1688, 2157
TauI GCSGC 1 cut(s) 3074
Tru1I TTAA 8 cut(s) 888, 989, 1472, 1727, 1971, 2175, 2352, 2717
Tru9I TTAA 8 cut(s) 888, 989, 1472, 1727, 1971, 2175, 2352, 2717
TscAI CASTG 6 cut(s) 388, 499, 1294, 1798, 2244, 2445
TseFI GTSAC 5 cut(s) 244, 374, 1069, 1272, 2552
Tsp45I GTSAC 5 cut(s) 244, 374, 1069, 1272, 2552
TspGWI ACGGA 3 cut(s) 263, 1875, 2540
TspRI CASTG 6 cut(s) 388, 499, 1294, 1798, 2244, 2445
Tth111I GACNNNGTC 1 cut(s) 760
Vha464I CTTAAG 3 cut(s) 887, 2174, 2351
VpaK11BI GGWCC 2 cut(s) 213, 1580
VspI ATTAAT 1 cut(s) 1727
XapI RAATTY 6 cut(s) 708, 991, 1108, 1474, 1846, 2329
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 3 cut(s) 228, 2672, 2765
XhoI CTCGAG 2 cut(s) 28, 3015
XmnI GAANNNNTTC 1 cut(s) 1715
XspI CTAG 7 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 3147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.