Rw4G006010
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015802
Physical Location & Seq
Reverse (-)
12167010 .. 12170573
3564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006010.1

Sequence Viewer

Length: 3435 bp
ATGTCGTCTGTTTCATCGTTACCCAGCTCGAGGCAATCCTTCTCTTCTTCTTGTTCTTCAATAATCTCCATTATCTATCTATTTCTCATTTCGTCGGCCTTTGCCTCAAACCATGAAGCCTCTCTTCTCTTCTCTTGGCTGCATTCCTCTACTTCTTTCTCTTCTTCTTCCTTTTCGAACTGGAACATTCTCGACTCTAATCCATGTAACTGGTCCTCCATAACATGTTCTCCAAACGGTTTTGTCAAAGAAATCACTATCCAGTCCATCCCTCTTGAGCTTCCAATCCCATCTAATCTTTCCTCCTTCACCTCCCTTCAAAAACTCGTCATTTCCGGCGGTAATCTCACCGGAAAAATCCCCTCCGACATCGGTGACTGCACTGAGTTACAAGTCATTGACCTAAGCTCAAACTCACTCGTGGGTTCGATACCTTTATCCATTGGAAGGCTTCAAAACCTCCAGGACTTGATTCTCAACTCAAACCAGCTCAGTGGGAAAATCCCAGTTGAGTTAAGCGACTGCATTGGCCTAAAGAATCTTGTCATTTTCGATAATCAGTTTTCCGGGAATATCCCTCCGGAGCTCGGAAAACTGGCCGGTCTTGAAGTGCTGAGAGCAGGAGGGAACAAAAACATTGGTGGGAGAATCCCTGATGAGCTTGGAGACTGCACCAACTTGACTGTTTTGGGCTTGGCTGATACTCAAGTTTCGGGTTCTTTGCCGGCCTCGTTGGGTAAGCTTAGCAAGCTTCAGACGCTGTCTATATACACCACAATGATCTCTGGTGAGATTCCTGCTGAGATAGGTAACTGTTCTGAGCTTGTGAACTTGTTTCTTTATGAAAACAGTCTATCCGGTTCGATTCCACCGGAGCTCGGTAGGCTTAAGAAGCTTGAGCAGTTGTTGTTGTGGCAGAATAGTCTTGTTGGGGTGATCCCTGAAGAGATTGGGAAATGTAGTAGCTTGAAAATGATTGATTTTTCATTAAATTCTTTGTCTGGTACCGTTCCATTGTCTCTAGGAGGGCTGTCAAACCTCGAGGAGTTTATGATTAGTAACAACAATGTCACCGGTTCAATACCTTCCAGTCTTTGCAATGCAACGAATTTGATTCAGTTGCAGCTTGATACAAATCAGATCTCCGGTTTGATTCCACCGGAGATTGGGAAGTTGTCAAAGCTGACTGTGTTCTTTGCTTGGCAAAACCAGCTTGAAGGAAGCATTCCTTCATCTTTGTCTAGTTTGAGTAATCTTCAAGCTCTAGATTTGTCACACAATTCACTCACTGGTAGCATACCTTCTGGCTTGTTTCAGCTGCAAAACCTCACAAAGATTCTCTTGATTTCTAATGATATTTCGGGCTCAATCCCACCCGAAATTGGCAACTGCAGCTCTCTTGTAAGGCTGAGGCTTGGAAATAACAGGATTACTGGTGGGATTCCTAGAGGCATTGGAAATCTCAGGAGCTTAAATTTTCTTGATCTTTCAGGGAATCGACTTTCTGGGTCAATTCCTGATGAGATTGGGAGTTGCACAGAGCTACAAATGATAGACCTCAGAAACAATACTTTAGAGGGTCCCCTGCCTAATTCATTGTCATCACTCTCAGGACTTCAAGTCTTGGATGTCTCCATCAATCAATTTTGGGGCCAGATACCTGCAAGCTTGGCTCGCCTTGTTACGTTGAACACGCTTATTCTGAGCAGGAACTCATTCTCTGGATTAATACCTGCATCACTCGGCCTATGTTCGAGTCTGCAATTGCTTGATCTTAGCAGCAACAAGCTCACTGGCAACATTCCTGTGGAGCTTGGCAAAATTGAATCCCTTGAAATTACTCTAAATTTGAGCTGCAATGGACTGTCTGGATCAATCCCACCTCAAATATCAGCACTCAACAAGCTTTTCGTACTAGACCTTTCACATAACCAGCTTGAAGGGGATTTGAGTCCACTCTCTTCTGGCCTTAACACTCTTGATTCTCTAAATGTGTCTTACAATAACCTCGTTGGCTATCTTCCAGACAACAAGCTTTTCAGACAACTGTCTCCAATGGATTTAGCCGGAAATGAAGGCCTCTGTGCTTCAAACCGGGACTCGTGTTTCTTGAGTGATGTTGGCAGGTCAGGACTATCAAGGAACCAAAATGACGTAAGGCGGTCAAGGAGGCTTAAGCTGGCAATTGCATTGCTGATCACCTTGACAGTTGCAATGGTTGTTATGGGGATAATTGCAGTGATTCGAGCACAAAGGACTATTAGGGATGACGATGATTCAGAGTTGGGGAGCTCATGGGCATGGCAATTCACTCCATTCCAGAAGCTAAATTTCTCGGTTGACCAAGTGCTTAAGAGCCTAGTGGATGCCAATGTGATTGGAAAAGGGTGTTCTGGGGTTGTTTATCGTGCTGATATGGACAATGGTGAAGTCATTGCAGTGAAGAAGCTCTGGCCAACTACAGTTGCTGCAGACAATGGATGTTGCAATGATGAAAAATGTGGAGTTCGTGATTCGTTCTCAGCAGAGGTCAAAACACTTGGCTCAATCCGTCACAAGAACATTGTTAGGTTCTTGGGATGTTGCTGGAATAGGAACACAAGGCTGCTCATGTATGACTATATGCCTAATGGAAGCTTGGGCAGTATTCTCCATGAGAGGACAGGACATGCCTTTGAGTGGGAGCTTAGGTACCGAGTTTTGTTGGGTGCTGCTCAAGGCATTGCCTACTTGCACCATGATTGTGTTCCTCCAATTGTTCACAGAGATATCAAAGCCAACAACATCCTCATTGGCCTAGAGTTTGAGGCTTACATTGCTGATTTTGGTCTTGCAAAACTTGTTGATGAGGGAAATTTCGCTCGATCATCTAATACAATTGCTGGTTCATATGGCTACATTGCTCCTGAATATGGATATATGATGAAGATTACAGAGAAGAGTGATGTTTATAGCTATGGTGTAGTTGTATTGGAAGTTCTAACAGGGAAGCAACCAATAGATCCAACAATACCAGATGGGCTACATGTAGTGGATTGGGTGAGACGGAAGAGAGGAAATGTTGAAGTGCTTGACCCAATCCTACTATCAAGACCAGAATCAGAAATAGAGGAAATGATGCAAGCATTAGGCATAGCCCTGTTATGTGTAAACTCCTCTCCAGTTGAAAGGCCAACAATGAAAGATGTAGCTGCAATGCTCAAGGAAATCAGGCATGAAAGGGAAGAGTATGCTAAGGTCGATGTGCTTCTCAAAGGGTCTCCGGCGAATGATGCTGCTCGAGAAAATAAGAACTCTGCTGGAGTTTTGGAAACATCATCATCATCGGCAGCAGCGGCAAAAAGCTTGTATGCTAAAAGCAATAACACAAGCTTTTCTGCATCCACTCTTCTTTACTCATCTTCTTCCTCTAGTGCAAAAATGGGTTTCAAGTGA

Protein Analysis

1144

Amino Acids

123.21

Weight (kDa)

5.41

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 102 - 207 1.5e-06 Leucine-rich repeat region
LRR_14 PF23598 220 - 306 5.2e-09 Leucine-rich repeat region
LRR_14 PF23598 310 - 427 1.9e-07 Leucine-rich repeat region
LRR_14 PF23598 495 - 620 2.8e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 791 - 1066 6.4e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 791 - 1064 4.9e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24240
fragaria_vesca FvH4_4g05730
malus_domestica MD00G1164700.v1.1 MD16G1236300.v1.1
prunus_persica Prupe.1G060700_v2.0.a1
pyrus_communis pycom13g20340
rosa_chinensis RchiOBHm_Chr4g0396901
rosa_laevigata RLG00000009492
rosa_multiflora Rmu_sc0008929.1_g000009
rosa_roxburghii Rroxscaffold_5G00341810
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4BG071300 Rh4CG079500 Rh4DG068900
rosa_wichuraiana Rw4G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1040
Acc36I ACCTGC 3 cut(s) 1669, 1741, 2115
Acc65I GGTACC 2 cut(s) 1004, 2691
AccB1I GGYRCC 2 cut(s) 1004, 2691
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 3 cut(s) 339, 2161, 3335
AclWI GGATC 3 cut(s) 931, 1879, 2996
AcoI YGGCCR 2 cut(s) 597, 2453
AcsI RAATTY 6 cut(s) 991, 1108, 1474, 1846, 2329, 2854
AcuI CTGAAG 2 cut(s) 737, 963
AfaI GTAC 3 cut(s) 1006, 1914, 2693
AfiI CCNNNNNNNGG 8 cut(s) 30, 447, 587, 878, 1166, 1382, 2679, 2912
AflII CTTAAG 3 cut(s) 887, 2174, 2351
AflIII ACRYGT 2 cut(s) 224, 3025
AgeI ACCGGT 1 cut(s) 1073
AhdI GACNNNNNGTC 1 cut(s) 1619
AjnI CCWGG 1 cut(s) 462
Alw21I GWGCWC 4 cut(s) 588, 879, 2251, 2294
Alw26I GTCTC 6 cut(s) 660, 1023, 1636, 2055, 3037, 3264
AlwI GGATC 3 cut(s) 931, 1879, 2996
AlwNI CAGNNNCTG 2 cut(s) 760, 2468
Ama87I CYCGRG 3 cut(s) 28, 1040, 3279
Aor13HI TCCGGA 1 cut(s) 580
ApoI RAATTY 6 cut(s) 991, 1108, 1474, 1846, 2329, 2854
AseI ATTAAT 1 cut(s) 1727
AsiGI ACCGGT 1 cut(s) 1073
Asp700I GAANNNNTTC 1 cut(s) 1715
Asp718I GGTACC 2 cut(s) 1004, 2691
AspS9I GGNCC 3 cut(s) 213, 1580, 1651
AsuC2I CCSGG 2 cut(s) 568, 2096
AsuHPI GGTGA 9 cut(s) 301, 340, 386, 800, 946, 1063, 2191, 2438, 3052
AsuII TTCGAA 1 cut(s) 176
AvaI CYCGRG 3 cut(s) 28, 1040, 3279
AvaII GGWCC 2 cut(s) 213, 1580
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BalI TGGCCA 1 cut(s) 2455
BanI GGYRCC 2 cut(s) 1004, 2691
BanII GRGCYC 4 cut(s) 588, 879, 1367, 2294
BarI GAAGNNNNNNTAC 2 cut(s) 1285, 1317
BauI CACGAG 2 cut(s) 419, 2101
Bbv12I GWGCWC 4 cut(s) 588, 879, 2251, 2294
BbvCI CCTCAGC 1 cut(s) 1409
BccI CCATC 4 cut(s) 275, 298, 1643, 3011
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 1 cut(s) 2196
BcnI CCSGG 2 cut(s) 568, 2096
BcoDI GTCTC 6 cut(s) 660, 1023, 1636, 2055, 3037, 3264
BfaI CTAG 8 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 2798, 3411
BfmI CTRYAG 3 cut(s) 1390, 2460, 2469
BfrI CTTAAG 3 cut(s) 887, 2174, 2351
BfuAI ACCTGC 3 cut(s) 1669, 1741, 2115
BglII AGATCT 1 cut(s) 1140
BlpI GCTNAGC 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 464, 568, 2096
Bme18I GGWCC 2 cut(s) 213, 1580
BmeRI GACNNNNNGTC 1 cut(s) 1619
BmeT110I CYCGRG 3 cut(s) 28, 1040, 3279
BmgT120I GGNCC 3 cut(s) 213, 1580, 1651
BmiI GGNNCC 6 cut(s) 1006, 1581, 1582, 1652, 2144, 2693
BmrFI CCNGG 3 cut(s) 464, 568, 2096
BmrI ACTGGG 1 cut(s) 500
BmsI GCATC 5 cut(s) 1745, 2356, 3108, 3262, 3389
BmuI ACTGGG 1 cut(s) 500
BoxI GACNNNNGTC 1 cut(s) 2047
BpmI CTGGAG 3 cut(s) 446, 3144, 3321
Bpu10I CCTNAGC 4 cut(s) 404, 1409, 2687, 3234
Bpu1102I GCTNAGC 1 cut(s) 743
Bpu14I TTCGAA 1 cut(s) 176
BpuEI CTTGAG 6 cut(s) 296, 690, 917, 2131, 2700, 3185
BpuMI CCSGG 2 cut(s) 568, 2096
BsaBI GATNNNNATC 1 cut(s) 1134
BsaI GGTCTC 1 cut(s) 3264
BsaWI WCCGGW 7 cut(s) 350, 580, 857, 871, 1073, 1145, 1159
BsaXI ACNNNNNCTCC 6 cut(s) 200, 214, 230, 244, 2675, 2705
Bsc4I CCNNNNNNNGG 8 cut(s) 30, 447, 587, 878, 1166, 1382, 2679, 2912
Bse118I RCCGGY 3 cut(s) 599, 724, 1073
Bse8I GATNNNNATC 1 cut(s) 1134
BseAI TCCGGA 1 cut(s) 580
BseBI CCWGG 1 cut(s) 464
BseGI GGATG 8 cut(s) 267, 1633, 2272, 2371, 2486, 2585, 2784, 3380
BseJI GATNNNNATC 1 cut(s) 1134
BseLI CCNNNNNNNGG 8 cut(s) 30, 447, 587, 878, 1166, 1382, 2679, 2912
BseRI GAGGAG 2 cut(s) 1058, 3145
BseYI CCCAGC 1 cut(s) 23
BsgI GTGCAG 2 cut(s) 364, 655
BshNI GGYRCC 2 cut(s) 1004, 2691
BshTI ACCGGT 1 cut(s) 1073
BsiHKAI GWGCWC 4 cut(s) 588, 879, 2251, 2294
BsiHKCI CYCGRG 3 cut(s) 28, 1040, 3279
BslFI GGGAC 2 cut(s) 1566, 2111
BslI CCNNNNNNNGG 8 cut(s) 30, 447, 587, 878, 1166, 1382, 2679, 2912
BsmAI GTCTC 6 cut(s) 660, 1023, 1636, 2055, 3037, 3264
BsmBI CGTCTC 1 cut(s) 3037
BsmFI GGGAC 2 cut(s) 1566, 2111
BsmI GAATGC 2 cut(s) 142, 1224
Bso31I GGTCTC 1 cut(s) 3264
BsoBI CYCGRG 3 cut(s) 28, 1040, 3279
Bsp119I TTCGAA 1 cut(s) 176
Bsp1286I GDGCHC 5 cut(s) 588, 879, 1367, 2251, 2294
Bsp13I TCCGGA 1 cut(s) 580
Bsp143I GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
Bsp1720I GCTNAGC 1 cut(s) 743
BspACI CCGC 3 cut(s) 339, 2161, 3335
BspEI TCCGGA 1 cut(s) 580
BspLI GGNNCC 6 cut(s) 1006, 1581, 1582, 1652, 2144, 2693
BspMAI CTGCAG 2 cut(s) 1394, 2473
BspMI ACCTGC 3 cut(s) 1669, 1741, 2115
BspPI GGATC 3 cut(s) 931, 1879, 2996
BspT104I TTCGAA 1 cut(s) 176
BspT107I GGYRCC 2 cut(s) 1004, 2691
BspTI CTTAAG 3 cut(s) 887, 2174, 2351
BspTNI GGTCTC 1 cut(s) 3264
BsrFI RCCGGY 3 cut(s) 599, 724, 1073
BssAI RCCGGY 3 cut(s) 599, 724, 1073
BssMI GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
BssSI CACGAG 2 cut(s) 419, 2101
Bst2BI CACGAG 2 cut(s) 419, 2101
Bst2UI CCWGG 1 cut(s) 464
BstAFI CTTAAG 3 cut(s) 887, 2174, 2351
BstBI TTCGAA 1 cut(s) 176
BstC8I GCNNGC 6 cut(s) 726, 749, 1666, 1675, 2181, 3123
BstF5I GGATG 8 cut(s) 267, 1633, 2272, 2371, 2486, 2585, 2784, 3380
BstKTI GATC 9 cut(s) 783, 939, 1143, 1486, 1774, 1874, 2199, 2867, 3004
BstMAI GTCTC 6 cut(s) 660, 1023, 1636, 2055, 3037, 3264
BstMBI GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 3 cut(s) 228, 2672, 3029
BstPAI GACNNNNGTC 1 cut(s) 2047
BstSCI CCNGG 3 cut(s) 462, 566, 2094
BstSFI CTRYAG 3 cut(s) 1390, 2460, 2469
BstX2I RGATCY 2 cut(s) 1140, 3001
BstXI CCANNNNNNTGG 2 cut(s) 210, 494
BstYI RGATCY 2 cut(s) 1140, 3001
BtsCI GGATG 8 cut(s) 267, 1633, 2272, 2371, 2486, 2585, 2784, 3380
BtsI GCAGTG 2 cut(s) 2244, 2445
BtsIMutI CAGTG 6 cut(s) 381, 499, 1287, 1791, 2244, 2445
BveI ACCTGC 3 cut(s) 1669, 1741, 2115
Cac8I GCNNGC 6 cut(s) 726, 749, 1666, 1675, 2181, 3123
CaiI CAGNNNCTG 2 cut(s) 760, 2468
Cfr10I RCCGGY 3 cut(s) 599, 724, 1073
Cfr13I GGNCC 3 cut(s) 213, 1580, 1651
CseI GACGC 1 cut(s) 766
Csp6I GTAC 3 cut(s) 1005, 1913, 2692
CspAI ACCGGT 1 cut(s) 1073
CviQI GTAC 3 cut(s) 1005, 1913, 2692
DpnI GATC 9 cut(s) 782, 938, 1142, 1485, 1773, 1873, 2198, 2866, 3003
DpnII GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
DriI GACNNNNNGTC 1 cut(s) 1619
EaeI YGGCCR 2 cut(s) 597, 2453
Eam1105I GACNNNNNGTC 1 cut(s) 1619
Ecl136II GAGCTC 3 cut(s) 586, 877, 2292
Eco147I AGGCCT 1 cut(s) 2079
Eco24I GRGCYC 4 cut(s) 588, 879, 1367, 2294
Eco31I GGTCTC 1 cut(s) 3264
Eco32I GATATC 1 cut(s) 2770
Eco47I GGWCC 2 cut(s) 213, 1580
Eco53kI GAGCTC 3 cut(s) 586, 877, 2292
Eco57I CTGAAG 2 cut(s) 737, 963
Eco88I CYCGRG 3 cut(s) 28, 1040, 3279
EcoICRI GAGCTC 3 cut(s) 586, 877, 2292
EcoO109I RGGNCCY 1 cut(s) 1580
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 2770
EcoT38I GRGCYC 4 cut(s) 588, 879, 1367, 2294
Esp3I CGTCTC 1 cut(s) 3037
FalI AAGNNNNNCTT 6 cut(s) 108, 140, 1213, 1245, 1325, 1357
FaqI GGGAC 2 cut(s) 1566, 2111
FauNDI CATATG 1 cut(s) 2890
FbaI TGATCA 1 cut(s) 2196
FokI GGATG 8 cut(s) 254, 1640, 2279, 2378, 2493, 2592, 2771, 3367
FriOI GRGCYC 4 cut(s) 588, 879, 1367, 2294
FspBI CTAG 8 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 2798, 3411
GsaI CCCAGC 1 cut(s) 27
GsuI CTGGAG 3 cut(s) 446, 3144, 3321
HgaI GACGC 1 cut(s) 766
HincII GTYRAC 1 cut(s) 2341
HindII GTYRAC 1 cut(s) 2341
HindIII AAGCTT 9 cut(s) 740, 749, 893, 1666, 1904, 2033, 2635, 3343, 3370
HphI GGTGA 9 cut(s) 301, 340, 386, 800, 946, 1063, 2191, 2438, 3052
Hpy166II GTNNAC 5 cut(s) 829, 1955, 2341, 2761, 3151
Hpy8I GTNNAC 5 cut(s) 829, 1955, 2341, 2761, 3151
Hpy99I CGWCG 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 1685, 2154
HpySE526I ACGT 2 cut(s) 1685, 2154
KflI GGGWCCC 1 cut(s) 1580
Kpn2I TCCGGA 1 cut(s) 580
KpnI GGTACC 2 cut(s) 1008, 2695
KroI GCCGGC 1 cut(s) 724
KroNI GCCGGC 1 cut(s) 726
Ksp22I TGATCA 1 cut(s) 2196
Kzo9I GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
LmnI GCTCC 7 cut(s) 583, 874, 1467, 1810, 2289, 2683, 2908
LweI GCATC 5 cut(s) 1745, 2356, 3108, 3262, 3389
MaeI CTAG 8 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 2798, 3411
MaeII ACGT 2 cut(s) 1685, 2154
MalI GATC 9 cut(s) 782, 938, 1142, 1485, 1773, 1873, 2198, 2866, 3003
MboI GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
MfeI CAATTG 4 cut(s) 1763, 2184, 2754, 2877
MflI RGATCY 2 cut(s) 1140, 3001
MhlI GDGCHC 5 cut(s) 588, 879, 1367, 2251, 2294
MlsI TGGCCA 1 cut(s) 2455
MluNI TGGCCA 1 cut(s) 2455
MlyI GAGTC 4 cut(s) 188, 1765, 1960, 2093
MmeI TCCRAC 2 cut(s) 390, 3029
Mox20I TGGCCA 1 cut(s) 2455
MroI TCCGGA 1 cut(s) 580
MroNI GCCGGC 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 1715
MscI TGGCCA 1 cut(s) 2455
MseI TTAA 8 cut(s) 515, 888, 989, 1472, 1727, 1971, 2175, 2352
MslI CAYNNNNRTG 5 cut(s) 776, 1805, 2299, 2438, 2742
Msp20I TGGCCA 1 cut(s) 2455
MspA1I CMGCKG 2 cut(s) 1318, 3335
MspCI CTTAAG 3 cut(s) 887, 2174, 2351
MspR9I CCNGG 3 cut(s) 464, 568, 2096
MunI CAATTG 4 cut(s) 1763, 2184, 2754, 2877
Mva1269I GAATGC 2 cut(s) 142, 1224
MvaI CCWGG 1 cut(s) 464
NaeI GCCGGC 1 cut(s) 726
NciI CCSGG 2 cut(s) 568, 2096
NdeI CATATG 1 cut(s) 2890
NdeII GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
NgoMIV GCCGGC 1 cut(s) 724
NlaIV GGNNCC 6 cut(s) 1006, 1581, 1582, 1652, 2144, 2693
NmeAIII GCCGAG 1 cut(s) 1722
NmuCI GTSAC 4 cut(s) 374, 1069, 1272, 2552
NspI RCATGY 3 cut(s) 228, 2672, 3029
NspV TTCGAA 1 cut(s) 176
PaeR7I CTCGAG 3 cut(s) 28, 1040, 3279
PceI AGGCCT 1 cut(s) 2079
PciI ACATGT 2 cut(s) 224, 3025
PctI GAATGC 2 cut(s) 142, 1224
PdiI GCCGGC 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 1715
PflFI GACNNNGTC 1 cut(s) 760
PfoI TCCNGGA 2 cut(s) 462, 566
PinAI ACCGGT 1 cut(s) 1073
PleI GAGTC 4 cut(s) 188, 1764, 1959, 2093
PpsI GAGTC 4 cut(s) 188, 1764, 1959, 2093
PpuMI RGGWCCY 1 cut(s) 1580
PscI ACATGT 2 cut(s) 224, 3025
PshAI GACNNNNGTC 1 cut(s) 2047
PshBI ATTAAT 1 cut(s) 1727
Psp124BI GAGCTC 3 cut(s) 588, 879, 2294
Psp5II RGGWCCY 1 cut(s) 1580
Psp6I CCWGG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 6 cut(s) 1006, 1581, 1582, 1652, 2144, 2693
PspPI GGNCC 3 cut(s) 213, 1580, 1651
PspPPI RGGWCCY 1 cut(s) 1580
PspXI VCTCGAGB 2 cut(s) 28, 1040
PsrI GAACNNNNNNTAC 2 cut(s) 2961, 2993
PstI CTGCAG 2 cut(s) 1394, 2473
PstNI CAGNNNCTG 2 cut(s) 760, 2468
PsuI RGATCY 2 cut(s) 1140, 3001
PsyI GACNNNGTC 1 cut(s) 760
PvuII CAGCTG 1 cut(s) 1318
RsaI GTAC 3 cut(s) 1006, 1914, 2693
RsaNI GTAC 3 cut(s) 1005, 1913, 2692
RseI CAYNNNNRTG 5 cut(s) 776, 1805, 2299, 2438, 2742
SacI GAGCTC 3 cut(s) 588, 879, 2294
SaqAI TTAA 8 cut(s) 515, 888, 989, 1472, 1727, 1971, 2175, 2352
Sau3AI GATC 9 cut(s) 780, 936, 1140, 1483, 1771, 1871, 2196, 2864, 3001
Sau96I GGNCC 3 cut(s) 213, 1580, 1651
SchI GAGTC 4 cut(s) 188, 1765, 1960, 2093
ScrFI CCNGG 3 cut(s) 464, 568, 2096
SduI GDGCHC 5 cut(s) 588, 879, 1367, 2251, 2294
SfaNI GCATC 5 cut(s) 1745, 2356, 3108, 3262, 3389
SfcI CTRYAG 3 cut(s) 1390, 2460, 2469
Sfr274I CTCGAG 3 cut(s) 28, 1040, 3279
SfuI TTCGAA 1 cut(s) 176
SinI GGWCC 2 cut(s) 213, 1580
SlaI CTCGAG 3 cut(s) 28, 1040, 3279
SmiMI CAYNNNNRTG 5 cut(s) 776, 1805, 2299, 2438, 2742
SseBI AGGCCT 1 cut(s) 2079
SsiI CCGC 3 cut(s) 339, 2161, 3335
SspMI CTAG 8 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 2798, 3411
SstI GAGCTC 3 cut(s) 588, 879, 2294
StuI AGGCCT 1 cut(s) 2079
StyD4I CCNGG 3 cut(s) 462, 566, 2094
TaiI ACGT 2 cut(s) 1688, 2157
TauI GCSGC 1 cut(s) 3338
Tru1I TTAA 8 cut(s) 515, 888, 989, 1472, 1727, 1971, 2175, 2352
Tru9I TTAA 8 cut(s) 515, 888, 989, 1472, 1727, 1971, 2175, 2352
TscAI CASTG 6 cut(s) 388, 499, 1294, 1798, 2244, 2445
TseFI GTSAC 4 cut(s) 374, 1069, 1272, 2552
Tsp45I GTSAC 4 cut(s) 374, 1069, 1272, 2552
TspGWI ACGGA 2 cut(s) 2540, 3061
TspRI CASTG 6 cut(s) 388, 499, 1294, 1798, 2244, 2445
Tth111I GACNNNGTC 1 cut(s) 760
Vha464I CTTAAG 3 cut(s) 887, 2174, 2351
VpaK11BI GGWCC 2 cut(s) 213, 1580
VspI ATTAAT 1 cut(s) 1727
XapI RAATTY 6 cut(s) 991, 1108, 1474, 1846, 2329, 2854
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 3 cut(s) 228, 2672, 3029
XhoI CTCGAG 3 cut(s) 28, 1040, 3279
XmnI GAANNNNTTC 1 cut(s) 1715
XspI CTAG 8 cut(s) 1022, 1242, 1265, 1446, 1916, 2360, 2798, 3411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.