MD16G1236300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015802
Physical Location & Seq
Forward (+)
24712548 .. 24716264
3717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1236300.v1.1.491

Sequence Viewer

Length: 3450 bp
ATGTCTACAGTGCTGCCGCCCAGCTCGAGGCAATCCTTCCCCATCTTCAGCACCTCCTCCTCCTCCCACTCTTCTGTTTTTCTCTGTCTCACCCTATTTTTACTCTACGCACCCACCTTTGCTTCTGCCGCACATCCTGAAGCCACCCTTCTCTTCTCCTGGCTCCATTCCTCTGCTGAGCCGCCACCTTCCTTCTTCTCCAACTGGAACCTCCTCCATCCCAATCCATGCAACTGGTCCTCCATAACATGTTCCCCTCAAGGCCACGTCACCGAAATCACAATCCAATACATCCCTCTCCAGCTTCCACTTCCAACCAGTCTCTCCTCCTTTCCCTCCCTCCGAAAACTCACCATTTCAGGTGCCAATCTCACCGGAACCATCCCCGCCGACATCGGTGAATGCACCGAGCTGCAAGTCATCGACCTCAGCTCAAACTGTCTGGTGGGTTCAATCCCGCCCAGCGTTTCAAGGCTCCGAAATCTCCAAGACTTGATCTTGAACTCCAATCAGCTCACTGGGAAAATCCCAGTTGAGCTCGGCACCTGCATTGGACTAAAAAACCTCCTTCTCTTCGACAACCAGCTAACCGGGTCGATTACGGCGGAGCTCGGGAAGCTCACTAGCCTTGCAGTTCTGAGAGCAGGAGGTAACAAATACATTGGTGGGAAAATCCCAGAGGAGCTCGGAGGCTGCAGCAATTTGACTGTCTTGGGGTTGGCTGACACCCAAGTTTCAGGCACTTTGCCTGCCTCATTGGGTAAGCTTAGCCATCTCCACACCCTGTCTATTTACACCACAATGATCTCCGGTGAAATCCCACCTGAAATAGGTAACTGCTCTGAGCTTGTCAACTTGTTTCTTTACGAAAATAGCCTCTCCGGTTCGATTCCACCGGAGCTTGGAAAGCTTAAGAAGCTGGAGCAGTTGTTATTGTGGCAAAATAGTCTTTCTGGTCCAATCCCAGAAGAGATTGGGAATTGTAGCAGCTTGAGAATGATTGATTTTTCTCTAAATGCTTTGTCTGGTACTATGCCTTTGTCTTGGGGAGGCTTATCGAATCTTGAGGAGTTTATGATTAGTGACAACAATGTCACCGGTTCGATCCCTTCCAACCTTTCTAATTTGACGAACCTGATGCAGCTGCAGCTCGACACGAATCAGATCTCCGGTTTGATTCCACCGGAGATTGGGATGTTGTCGAAGCTGACTGTTCTTTTCGCTTGGCAAAACCAGCTTGAAGGAAGCATTCCTTCAACTCTGGCTAGTTGTAGCAATCTCCAAGCACTAGACTTGTCACACAATTCGCTCACCGGCACCATTCCTGCCGGATTGTTTCAGCTCAAAAACCTCACAAAGCTTCTTTTGATTTCGAATGACATTTCGGGTTTGATTCCACCAAGTATTGGCAACTGCAGCTCTCTGGTTCGGCTGCGGCTTGGGAACAACAGGATTGCTGGTGGGATTCCGAAAGCAATTGGAGACCTTAGGAGCTTAAACTTTCTTGACCTGTCTGGGAACAGCCTTTCTGGGCAAGTGCCTGATGAGATTGGGAGTTGCACAGAGCTACAGATGATAGACTTGAGCAACAATACTTTGGAAGGTCCCCTGCCTAACGCTTTGTCGTCACTATCAGGACTTCAAGTCTTGGATGTTTCGGTTAATCAGTTTTCGGAGCAGATACCAGCGAGCTTGGCTCGTCTTGTTTCGTTGAACAAGCTTATTTTGAGCAGGAACTTGTTCTCTGGAATGATACCTACATCGCTTGGCCTGTGTTCGAGTCTCCAATTGCTTGATCTTAGCAGCAACAAGCTCACTGGCTCAATCCCCGTGGAGCTTGGGAGGATTGAAGCCCTTGAAATCGCGCTAAATTTGAGCTGCAATGCACTCTCAGGCCCCATCCCACCTCAAATATCAGCACTCACCAAGCTTTCGATACTAGACCTTTCACATAACCAGCTTGATGGGGACTTGAGTCCACTTGCAGAGCTCAATAATCTTGTCTCGCTTAATGTGTCATATAACAAACTCAGTGGCTACCTTCCAGACAACAAGCTTTTCAGACAGTTGTTACCAATGGATTTGGCTGGAAACGAAGGCCTTTGCTCTTCAAATCGCGACTCATGCTTCCTGAGTGATGTTGGCCGCACAGGACTAACAAGAAATCAAAATGAAATAAGGCGGTCACGCAGGCTTAAGCTAGCAATTGCATTGCTGATCATCCTGACAGTTGCAATGGTGGTTTTGGGGATCCTTGCAGTGATTAGAGCGCGAAGAGCTATCAGAGATGATGATGAATCAGAATTGGGGAACTCGTGGGCTTGGCAGTTCACTCCATTCCAGAAGCTAAATTTCTCGGTTGAGCAAGTGCTTAGATGCCTGGTGGATGTCAATGTAATTGGAAAAGGGTGTTCTGGGGTTGTGTATCGTGCTGATATGGACAATGGCGAAGTCATTGCAGTGAAGAAGCTCTGGCCAGCAACAGTTGCTGCTGACAACGGATGTTGTAATGATGAAAAATGTGGAGTTCGCGATTCATTCTCAGCGGAGGTCAAAACACTTGGCTCAATACGTCACAAGAACATTGTTCGGTTCTTGGGTTGTTGCTGGAATAAGAACACAAGATTGCTCATGTACGATTACATGCCTAATGGAAGCTTGGGAAGTATTCTACATGAGAGGACAGGGCATGCCTTTGAATGGGAAGTTCGGTACCAAATTTTGTTGGGTGCAGCTCAAGGCATTGCCTATTTGCACCATGATTGTGTTCCTCCAATTGTTCACAGGGATATCAAGGCCAATAACATCCTCATTGGCCTTGAGTTTGAGCCTTACATTGCTGATTTTGGCCTAGCCAAACTCGTTGATGATGGCGATTTCGCTCAGTCCTCCAACACAGTTGCTGGTTCCTATGGCTATATTGCTCCTGAATATGGATACATGATGAAGATCACAGAGAAGAGCGATGTTTATAGCTATGGAGTTGTTGTATTGGAAGTCTTGACAGGGAAACAACCGATAGATCCAACAATACCAGAAGGGCAACATGTAGTGGATTGGGTAAGACAGAAGAGGGGAAGCATTGAAGTCCTCGACCCAAGCCTCATATCGAGACCCGAATCGGAGATAGAGGAAATGATGCAGGCATTAGGCATAGCCTTGTTGTGTGTAAACTCAACCCCTGATGAAAGACCAACCATGAAAGATGTGGCAGCAATGCTAAAGGAAGTCAAACATGAGAGGGAGGAGTATGCCAAGGTTGATATGCTCCTCAAAGGTTCGCCCGCGAATGATGCTCGCGAAAATAAGAACCCTAATGGAGTTTTGGCAACCTCATCATCGGCAGCAATGAAAAGCTTGTTTGCAAAAAGCAACAACACAATGTTTTCTGCATCCACATTGCTTTACTCATCATCCTTCTCTAGTGCCAATAAAATGGGGTTCAAGTGA

Protein Analysis

1150

Amino Acids

124.16

Weight (kDa)

5.45

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 108 - 215 8.1e-09 Leucine-rich repeat region
LRR_8 PF13855 112 - 172 1.4e-06 Leucine rich repeat
LRR_14 PF23598 228 - 339 2.3e-11 Leucine-rich repeat region
LRR_8 PF13855 402 - 461 1.5e-06 Leucine rich repeat
LRR_8 PF13855 450 - 509 3.4e-07 Leucine rich repeat
LRR_14 PF23598 469 - 691 6.4e-13 Leucine-rich repeat region
Pkinase PF00069 797 - 1071 8.9e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 798 - 1073 1.2e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24240
fragaria_vesca FvH4_4g05730
malus_domestica MD00G1164700.v1.1 MD16G1236300.v1.1
prunus_persica Prupe.1G060700_v2.0.a1
pyrus_communis pycom13g20340
rosa_chinensis RchiOBHm_Chr4g0396901
rosa_laevigata RLG00000009492
rosa_multiflora Rmu_sc0008929.1_g000009
rosa_roxburghii Rroxscaffold_5G00341810
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4BG071300 Rh4CG079500 Rh4DG068900
rosa_wichuraiana Rw4G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 554
AasI GACNNNNNNGTC 1 cut(s) 1091
Acc36I ACCTGC 1 cut(s) 554
Acc65I GGTACC 1 cut(s) 2712
AccB1I GGYRCC 4 cut(s) 362, 542, 1316, 2712
AccB7I CCANNNNNTGG 1 cut(s) 1406
AccI GTMKAC 1 cut(s) 5
AccII CGCG 6 cut(s) 1865, 2118, 2272, 2532, 3287, 3300
AclWI GGATC 4 cut(s) 1099, 2245, 2258, 3017
AcoI YGGCCR 2 cut(s) 2143, 2474
AcsI RAATTY 3 cut(s) 1870, 2350, 2718
AcuI CTGAAG 2 cut(s) 31, 159
AfaI GTAC 3 cut(s) 1030, 2636, 2714
AfiI CCNNNNNNNGG 7 cut(s) 27, 830, 902, 1190, 1406, 2700, 2933
AflII CTTAAG 2 cut(s) 911, 2195
AflIII ACRYGT 2 cut(s) 248, 3046
AgeI ACCGGT 1 cut(s) 1097
AhdI GACNNNNNGTC 1 cut(s) 1643
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 2 cut(s) 158, 2379
Alw21I GWGCWC 4 cut(s) 540, 612, 687, 1992
Alw26I GTCTC 6 cut(s) 92, 326, 1476, 1787, 2008, 3106
AlwI GGATC 4 cut(s) 1099, 2245, 2258, 3017
AlwNI CAGNNNCTG 2 cut(s) 2489, 2903
Ama87I CYCGRG 2 cut(s) 25, 611
AoxI GGCC 9 cut(s) 262, 1768, 1894, 2098, 2143, 2474, 2796, 2815, 2848
ApoI RAATTY 3 cut(s) 1870, 2350, 2718
AsiGI ACCGGT 1 cut(s) 1097
Asp700I GAANNNNTTC 1 cut(s) 1739
Asp718I GGTACC 1 cut(s) 2712
AspLEI GCGC 2 cut(s) 1867, 2272
AspS9I GGNCC 4 cut(s) 237, 956, 1604, 1895
AsuC2I CCSGG 1 cut(s) 592
AsuHPI GGTGA 9 cut(s) 82, 262, 343, 364, 410, 824, 1087, 1303, 1915
AsuII TTCGAA 1 cut(s) 1373
AsuNHI GCTAGC 1 cut(s) 2200
AvaI CYCGRG 2 cut(s) 25, 611
AvaII GGWCC 3 cut(s) 237, 956, 1604
AxyI CCTNAGG 1 cut(s) 1487
BalI TGGCCA 1 cut(s) 2476
BamHI GGATCC 1 cut(s) 2250
BanI GGYRCC 4 cut(s) 362, 542, 1316, 2712
BanII GRGCYC 4 cut(s) 540, 612, 687, 1992
BauI CACGAG 1 cut(s) 2314
Bbv12I GWGCWC 4 cut(s) 540, 612, 687, 1992
BbvCI CCTCAGC 1 cut(s) 428
BccI CCATC 7 cut(s) 50, 225, 389, 780, 1907, 1958, 2864
BceAI ACGGC 1 cut(s) 618
BcgI CGANNNNNNTGC 4 cut(s) 1120, 1154, 2438, 2472
BciT130I CCWGG 2 cut(s) 160, 2381
BciVI GTATCC 1 cut(s) 2930
BclI TGATCA 1 cut(s) 2217
BcnI CCSGG 1 cut(s) 592
BcoDI GTCTC 6 cut(s) 92, 326, 1476, 1787, 2008, 3106
BfaI CTAG 7 cut(s) 624, 1266, 1289, 1940, 2201, 2852, 3423
BfmI CTRYAG 5 cut(s) 6, 694, 1145, 1414, 1568
BfrI CTTAAG 2 cut(s) 911, 2195
BfuAI ACCTGC 1 cut(s) 554
BfuI GTATCC 1 cut(s) 2930
BglII AGATCT 1 cut(s) 1164
BlpI GCTNAGC 2 cut(s) 177, 767
Bme1390I CCNGG 3 cut(s) 160, 592, 2381
Bme18I GGWCC 3 cut(s) 237, 956, 1604
BmeRI GACNNNNNGTC 1 cut(s) 1643
BmeT110I CYCGRG 2 cut(s) 25, 611
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 4 cut(s) 237, 956, 1604, 1895
BmrFI CCNGG 3 cut(s) 160, 592, 2381
BmrI ACTGGG 2 cut(s) 524, 528
BmsI GCATC 5 cut(s) 1128, 2366, 3129, 3283, 3401
BmtI GCTAGC 1 cut(s) 2204
BmuI ACTGGG 2 cut(s) 524, 528
BoxI GACNNNNGTC 1 cut(s) 1974
BplI GAGNNNNNCTC 2 cut(s) 1681, 1713
BpmI CTGGAG 2 cut(s) 284, 941
Bpu10I CCTNAGC 1 cut(s) 428
Bpu1102I GCTNAGC 2 cut(s) 177, 767
Bpu14I TTCGAA 1 cut(s) 1373
BpuEI CTTGAG 7 cut(s) 243, 1012, 1085, 1603, 1993, 2721, 2840
BpuMI CCSGG 1 cut(s) 592
BsaBI GATNNNNATC 1 cut(s) 2948
BsaI GGTCTC 2 cut(s) 1476, 3106
BsaJI CCNNGG 2 cut(s) 1830, 3255
BsaWI WCCGGW 7 cut(s) 374, 809, 881, 895, 1097, 1169, 1183
BsaXI ACNNNNNCTCC 6 cut(s) 224, 254, 914, 944, 2973, 3003
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 830, 902, 1190, 1406, 2700, 2933
Bse118I RCCGGY 2 cut(s) 1097, 1313
Bse1I ACTGG 6 cut(s) 209, 239, 318, 523, 530, 1822
Bse21I CCTNAGG 1 cut(s) 1487
Bse3DI GCAATG 9 cut(s) 1888, 2210, 2241, 2454, 2742, 2835, 3222, 3354, 3398
Bse8I GATNNNNATC 1 cut(s) 2948
BseBI CCWGG 2 cut(s) 160, 2381
BseDI CCNNGG 2 cut(s) 1830, 3255
BseJI GATNNNNATC 1 cut(s) 2948
BseLI CCNNNNNNNGG 7 cut(s) 27, 830, 902, 1190, 1406, 2700, 2933
BseMI GCAATG 9 cut(s) 1888, 2210, 2241, 2454, 2742, 2835, 3222, 3354, 3398
BseMII CTCAG 9 cut(s) 168, 442, 629, 834, 1905, 2044, 2123, 2556, 2897
BseNI ACTGG 6 cut(s) 209, 239, 318, 523, 530, 1822
BseRI GAGGAG 9 cut(s) 46, 49, 52, 203, 316, 695, 1082, 3260, 3260
BseYI CCCAGC 2 cut(s) 20, 461
BsgI GTGCAG 1 cut(s) 2751
Bsh1236I CGCG 6 cut(s) 1865, 2118, 2272, 2532, 3287, 3300
BshFI GGCC 9 cut(s) 264, 1770, 1896, 2100, 2145, 2476, 2798, 2817, 2850
BshNI GGYRCC 4 cut(s) 362, 542, 1316, 2712
BshTI ACCGGT 1 cut(s) 1097
BsiHKAI GWGCWC 4 cut(s) 540, 612, 687, 1992
BsiHKCI CYCGRG 2 cut(s) 25, 611
BslFI GGGAC 2 cut(s) 1590, 1982
BslI CCNNNNNNNGG 7 cut(s) 27, 830, 902, 1190, 1406, 2700, 2933
BsmAI GTCTC 6 cut(s) 92, 326, 1476, 1787, 2008, 3106
BsmFI GGGAC 2 cut(s) 1590, 1982
BsmI GAATGC 2 cut(s) 407, 1248
BsnI GGCC 9 cut(s) 264, 1770, 1896, 2100, 2145, 2476, 2798, 2817, 2850
Bso31I GGTCTC 2 cut(s) 1476, 3106
BsoBI CYCGRG 2 cut(s) 25, 611
Bsp119I TTCGAA 1 cut(s) 1373
Bsp1286I GDGCHC 4 cut(s) 540, 612, 687, 1992
Bsp143I GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
Bsp1720I GCTNAGC 2 cut(s) 177, 767
Bsp68I TCGCGA 3 cut(s) 2118, 2532, 3300
BspANI GGCC 9 cut(s) 264, 1770, 1896, 2100, 2145, 2476, 2798, 2817, 2850
BspCNI CTCAG 9 cut(s) 169, 441, 630, 835, 1904, 2043, 2124, 2555, 2896
BspFNI CGCG 6 cut(s) 1865, 2118, 2272, 2532, 3287, 3300
BspMAI CTGCAG 3 cut(s) 698, 1149, 1418
BspMI ACCTGC 1 cut(s) 554
BspOI GCTAGC 1 cut(s) 2204
BspPI GGATC 4 cut(s) 1099, 2245, 2258, 3017
BspQI GCTCTTC 3 cut(s) 2113, 2269, 2954
BspT104I TTCGAA 1 cut(s) 1373
BspT107I GGYRCC 4 cut(s) 362, 542, 1316, 2712
BspTI CTTAAG 2 cut(s) 911, 2195
BspTNI GGTCTC 2 cut(s) 1476, 3106
BsrDI GCAATG 9 cut(s) 1888, 2210, 2241, 2454, 2742, 2835, 3222, 3354, 3398
BsrFI RCCGGY 2 cut(s) 1097, 1313
BsrI ACTGG 6 cut(s) 209, 239, 318, 523, 530, 1822
BssAI RCCGGY 2 cut(s) 1097, 1313
BssECI CCNNGG 2 cut(s) 1830, 3255
BssMI GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
BssSI CACGAG 1 cut(s) 2314
BssT1I CCWWGG 1 cut(s) 3255
Bst2BI CACGAG 1 cut(s) 2314
Bst2UI CCWGG 2 cut(s) 160, 2381
Bst4CI ACNGT 8 cut(s) 10, 440, 709, 1213, 2067, 2230, 2485, 2899
Bst6I CTCTTC 8 cut(s) 76, 158, 578, 963, 2113, 2269, 2954, 3065
BstAFI CTTAAG 2 cut(s) 911, 2195
BstAPI GCANNNNNTGC 1 cut(s) 2486
BstBI TTCGAA 1 cut(s) 1373
BstC8I GCNNGC 9 cut(s) 750, 1690, 2192, 2202, 2478, 2691, 3144, 3285, 3298
BstDSI CCRYGG 1 cut(s) 1830
BstENI CCTNNNNNAGG 1 cut(s) 828
BstFNI CGCG 6 cut(s) 1865, 2118, 2272, 2532, 3287, 3300
BstHHI GCGC 2 cut(s) 1867, 2272
BstKTI GATC 9 cut(s) 498, 807, 1107, 1167, 1798, 2220, 2253, 2952, 3025
BstMAI GTCTC 6 cut(s) 92, 326, 1476, 1787, 2008, 3106
BstMBI GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
BstNI CCWGG 2 cut(s) 160, 2381
BstNSI RCATGY 4 cut(s) 252, 2647, 2693, 3050
BstPAI GACNNNNGTC 1 cut(s) 1974
BstSCI CCNGG 3 cut(s) 158, 590, 2379
BstSFI CTRYAG 5 cut(s) 6, 694, 1145, 1414, 1568
BstUI CGCG 6 cut(s) 1865, 2118, 2272, 2532, 3287, 3300
BstX2I RGATCY 3 cut(s) 1164, 2250, 3022
BstXI CCANNNNNNTGG 3 cut(s) 234, 1964, 3436
BstYI RGATCY 3 cut(s) 1164, 2250, 3022
Bsu36I CCTNAGG 1 cut(s) 1487
BsuI GTATCC 1 cut(s) 2930
BsuRI GGCC 9 cut(s) 264, 1770, 1896, 2100, 2145, 2476, 2798, 2817, 2850
BtgI CCRYGG 1 cut(s) 1830
BtgZI GCGATG 2 cut(s) 1746, 2979
BtrI CACGTC 1 cut(s) 268
BtsI GCAGTG 2 cut(s) 2265, 2466
BtsIMutI CAGTG 6 cut(s) 15, 516, 1815, 2038, 2265, 2466
BtuMI TCGCGA 3 cut(s) 2118, 2532, 3300
BveI ACCTGC 1 cut(s) 554
Cac8I GCNNGC 9 cut(s) 750, 1690, 2192, 2202, 2478, 2691, 3144, 3285, 3298
CaiI CAGNNNCTG 2 cut(s) 2489, 2903
CfoI GCGC 2 cut(s) 1867, 2272
Cfr10I RCCGGY 2 cut(s) 1097, 1313
Cfr13I GGNCC 4 cut(s) 237, 956, 1604, 1895
Csp6I GTAC 3 cut(s) 1029, 2635, 2713
CspAI ACCGGT 1 cut(s) 1097
CspCI CAANNNNNGTGG 4 cut(s) 1812, 1847, 2014, 2049
CviQI GTAC 3 cut(s) 1029, 2635, 2713
DpnI GATC 9 cut(s) 497, 806, 1106, 1166, 1797, 2219, 2252, 2951, 3024
DpnII GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
DrdI GACNNNNNNGTC 1 cut(s) 1091
DriI GACNNNNNGTC 1 cut(s) 1643
DseDI GACNNNNNNGTC 1 cut(s) 1091
EaeI YGGCCR 2 cut(s) 2143, 2474
Eam1104I CTCTTC 8 cut(s) 76, 158, 578, 963, 2113, 2269, 2954, 3065
Eam1105I GACNNNNNGTC 1 cut(s) 1643
EarI CTCTTC 8 cut(s) 76, 158, 578, 963, 2113, 2269, 2954, 3065
EciI GGCGGA 1 cut(s) 620
Ecl136II GAGCTC 4 cut(s) 538, 610, 685, 1990
Eco130I CCWWGG 1 cut(s) 3255
Eco147I AGGCCT 1 cut(s) 2100
Eco24I GRGCYC 4 cut(s) 540, 612, 687, 1992
Eco31I GGTCTC 2 cut(s) 1476, 3106
Eco32I GATATC 1 cut(s) 2791
Eco47I GGWCC 3 cut(s) 237, 956, 1604
Eco53kI GAGCTC 4 cut(s) 538, 610, 685, 1990
Eco57I CTGAAG 2 cut(s) 31, 159
Eco81I CCTNAGG 1 cut(s) 1487
Eco88I CYCGRG 2 cut(s) 25, 611
EcoICRI GAGCTC 4 cut(s) 538, 610, 685, 1990
EcoNI CCTNNNNNAGG 1 cut(s) 828
EcoO109I RGGNCCY 2 cut(s) 1604, 1895
EcoRII CCWGG 2 cut(s) 158, 2379
EcoRV GATATC 1 cut(s) 2791
EcoT14I CCWWGG 1 cut(s) 3255
EcoT38I GRGCYC 4 cut(s) 540, 612, 687, 1992
ErhI CCWWGG 1 cut(s) 3255
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 1237, 1269
FaqI GGGAC 2 cut(s) 1590, 1982
FauI CCCGC 3 cut(s) 394, 465, 3292
FbaI TGATCA 1 cut(s) 2217
FblI GTMKAC 1 cut(s) 5
FriOI GRGCYC 4 cut(s) 540, 612, 687, 1992
FspBI CTAG 7 cut(s) 624, 1266, 1289, 1940, 2201, 2852, 3423
GlaI GCGC 2 cut(s) 1866, 2271
GsaI CCCAGC 2 cut(s) 24, 465
GsuI CTGGAG 2 cut(s) 284, 941
HaeIII GGCC 9 cut(s) 264, 1770, 1896, 2100, 2145, 2476, 2798, 2817, 2850
HhaI GCGC 2 cut(s) 1867, 2272
Hin6I GCGC 2 cut(s) 1865, 2270
HinP1I GCGC 2 cut(s) 1865, 2270
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HindIII AAGCTT 8 cut(s) 764, 908, 1358, 1718, 1928, 2054, 2656, 3355
HphI GGTGA 9 cut(s) 82, 262, 343, 364, 410, 824, 1087, 1303, 1915
Hpy166II GTNNAC 6 cut(s) 6, 853, 1979, 2331, 2782, 3172
Hpy8I GTNNAC 6 cut(s) 6, 853, 1979, 2331, 2782, 3172
HpyCH4III ACNGT 8 cut(s) 10, 440, 709, 1213, 2067, 2230, 2485, 2899
HpyCH4IV ACGT 2 cut(s) 267, 2572
HpySE526I ACGT 2 cut(s) 267, 2572
HspAI GCGC 2 cut(s) 1865, 2270
KpnI GGTACC 1 cut(s) 2716
Ksp22I TGATCA 1 cut(s) 2217
Kzo9I GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
LguI GCTCTTC 3 cut(s) 2113, 2269, 2954
LweI GCATC 5 cut(s) 1128, 2366, 3129, 3283, 3401
MaeI CTAG 7 cut(s) 624, 1266, 1289, 1940, 2201, 2852, 3423
MaeII ACGT 2 cut(s) 267, 2572
MalI GATC 9 cut(s) 497, 806, 1106, 1166, 1797, 2219, 2252, 2951, 3024
MboI GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
MfeI CAATTG 4 cut(s) 1476, 1787, 2205, 2775
MflI RGATCY 3 cut(s) 1164, 2250, 3022
MhlI GDGCHC 4 cut(s) 540, 612, 687, 1992
MlsI TGGCCA 1 cut(s) 2476
MluNI TGGCCA 1 cut(s) 2476
MlyI GAGTC 3 cut(s) 1789, 1984, 2114
MmeI TCCRAC 5 cut(s) 225, 338, 1137, 2916, 3050
Mox20I TGGCCA 1 cut(s) 2476
MroXI GAANNNNTTC 1 cut(s) 1739
MscI TGGCCA 1 cut(s) 2476
MseI TTAA 5 cut(s) 912, 1496, 1662, 2010, 2196
MslI CAYNNNNRTG 3 cut(s) 800, 2459, 2763
Msp20I TGGCCA 1 cut(s) 2476
MspA1I CMGCKG 2 cut(s) 1144, 2546
MspCI CTTAAG 2 cut(s) 911, 2195
MspR9I CCNGG 3 cut(s) 160, 592, 2381
MunI CAATTG 4 cut(s) 1476, 1787, 2205, 2775
Mva1269I GAATGC 2 cut(s) 407, 1248
MvaI CCWGG 2 cut(s) 160, 2381
MvnI CGCG 6 cut(s) 1865, 2118, 2272, 2532, 3287, 3300
NciI CCSGG 1 cut(s) 592
NdeII GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
NheI GCTAGC 1 cut(s) 2200
NmeAIII GCCGAG 1 cut(s) 519
NmuCI GTSAC 7 cut(s) 268, 1082, 1093, 1296, 1626, 2184, 2573
NruI TCGCGA 3 cut(s) 2118, 2532, 3300
NspI RCATGY 4 cut(s) 252, 2647, 2693, 3050
NspV TTCGAA 1 cut(s) 1373
PaeI GCATGC 1 cut(s) 2693
PaeR7I CTCGAG 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 554
PceI AGGCCT 1 cut(s) 2100
PciI ACATGT 2 cut(s) 248, 3046
PciSI GCTCTTC 3 cut(s) 2113, 2269, 2954
PctI GAATGC 2 cut(s) 407, 1248
PdmI GAANNNNTTC 1 cut(s) 1739
PfeI GAWTC 9 cut(s) 889, 1060, 1159, 1177, 1393, 1465, 2297, 2534, 3119
PflMI CCANNNNNTGG 1 cut(s) 1406
PinAI ACCGGT 1 cut(s) 1097
PleI GAGTC 3 cut(s) 1788, 1983, 2114
PpsI GAGTC 3 cut(s) 1788, 1983, 2114
PpuMI RGGWCCY 1 cut(s) 1604
PscI ACATGT 2 cut(s) 248, 3046
PshAI GACNNNNGTC 1 cut(s) 1974
Psp124BI GAGCTC 4 cut(s) 540, 612, 687, 1992
Psp5II RGGWCCY 1 cut(s) 1604
Psp6I CCWGG 2 cut(s) 158, 2379
PspFI CCCAGC 2 cut(s) 20, 461
PspGI CCWGG 2 cut(s) 158, 2379
PspPI GGNCC 4 cut(s) 237, 956, 1604, 1895
PspPPI RGGWCCY 1 cut(s) 1604
PspXI VCTCGAGB 1 cut(s) 25
PstI CTGCAG 3 cut(s) 698, 1149, 1418
PstNI CAGNNNCTG 2 cut(s) 2489, 2903
PsuI RGATCY 3 cut(s) 1164, 2250, 3022
PvuII CAGCTG 1 cut(s) 1144
RruI TCGCGA 3 cut(s) 2118, 2532, 3300
RsaI GTAC 3 cut(s) 1030, 2636, 2714
RsaNI GTAC 3 cut(s) 1029, 2635, 2713
RseI CAYNNNNRTG 3 cut(s) 800, 2459, 2763
SacI GAGCTC 4 cut(s) 540, 612, 687, 1992
SapI GCTCTTC 3 cut(s) 2113, 2269, 2954
SaqAI TTAA 5 cut(s) 912, 1496, 1662, 2010, 2196
Sau3AI GATC 9 cut(s) 495, 804, 1104, 1164, 1795, 2217, 2250, 2949, 3022
Sau96I GGNCC 4 cut(s) 237, 956, 1604, 1895
SchI GAGTC 3 cut(s) 1789, 1984, 2114
ScrFI CCNGG 3 cut(s) 160, 592, 2381
SduI GDGCHC 4 cut(s) 540, 612, 687, 1992
SfaNI GCATC 5 cut(s) 1128, 2366, 3129, 3283, 3401
SfcI CTRYAG 5 cut(s) 6, 694, 1145, 1414, 1568
Sfr274I CTCGAG 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 1373
SinI GGWCC 3 cut(s) 237, 956, 1604
SlaI CTCGAG 1 cut(s) 25
SmiMI CAYNNNNRTG 3 cut(s) 800, 2459, 2763
SphI GCATGC 1 cut(s) 2693
SseBI AGGCCT 1 cut(s) 2100
SspMI CTAG 7 cut(s) 624, 1266, 1289, 1940, 2201, 2852, 3423
SstI GAGCTC 4 cut(s) 540, 612, 687, 1992
StuI AGGCCT 1 cut(s) 2100
StyD4I CCNGG 3 cut(s) 158, 590, 2379
StyI CCWWGG 1 cut(s) 3255
TaaI ACNGT 8 cut(s) 10, 440, 709, 1213, 2067, 2230, 2485, 2899
TaiI ACGT 2 cut(s) 270, 2575
TauI GCSGC 5 cut(s) 19, 131, 184, 1438, 2148
TfiI GAWTC 9 cut(s) 889, 1060, 1159, 1177, 1393, 1465, 2297, 2534, 3119
Tru1I TTAA 5 cut(s) 912, 1496, 1662, 2010, 2196
Tru9I TTAA 5 cut(s) 912, 1496, 1662, 2010, 2196
TscAI CASTG 6 cut(s) 15, 523, 1822, 2038, 2265, 2466
TseFI GTSAC 7 cut(s) 268, 1082, 1093, 1296, 1626, 2184, 2573
Tsp45I GTSAC 7 cut(s) 268, 1082, 1093, 1296, 1626, 2184, 2573
TspDTI ATGAA 8 cut(s) 2187, 2310, 2526, 2529, 2960, 3201, 3215, 3365
TspGWI ACGGA 1 cut(s) 2514
TspRI CASTG 6 cut(s) 15, 523, 1822, 2038, 2265, 2466
Van91I CCANNNNNTGG 1 cut(s) 1406
Vha464I CTTAAG 2 cut(s) 911, 2195
VpaK11BI GGWCC 3 cut(s) 237, 956, 1604
XagI CCTNNNNNAGG 1 cut(s) 828
XapI RAATTY 3 cut(s) 1870, 2350, 2718
XceI RCATGY 4 cut(s) 252, 2647, 2693, 3050
XcmI CCANNNNNNNNNTGG 1 cut(s) 3205
XhoI CTCGAG 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 1739
XspI CTAG 7 cut(s) 624, 1266, 1289, 1940, 2201, 2852, 3423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.