pycom13g20340
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015802
Physical Location & Seq
Reverse (-)
16381289 .. 16384002
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20340.1

Sequence Viewer

Length: 2460 bp
ATGATTGATTTTTCTCTAAATTCTTTGTCTGGAACTATACCCTTGTCGTTAGGAGGGTTGTCGAATCTTGAGGAGTTTATGATTAGTGACAACAATGTCACCGGTTCGATCCCTTCAAACCTTTCTAATTTGACGAACCTGATGCAGCTGCAGCTCGACACCAATCAGATCTCCGGTTTGATTCCACCGGAGATAGGGATGTTGTCGAAGCTGATTGTTTTTTTCGCTTGGCAAAACCAGCTTGAAGGTAGCATTCCTTCAACTCTGGCTAGTTGTAGCAGTCTCCAAGCACTAGACTTGTCACACAATTCGCTCACGGGTAGCATTCCTGCCGGCTTGTTTCAGCTCAAAAACCTCACAAAGGTTCTTTTGATTTCGAATGACATCTCGGGTTTCATTCCACCAAGTATTGGTAACTGCAGCTCTCTGTTTCGGTTGCGGCTTGGGAACAACAGGATTGCTGGTGGGATTCCGAAAGCAATTGGAGACCTTAGGAGCTTAAACTTTCTTGACCTGTCCGGGAACCACCTTTCTGGGCAAGTGCCTGATGAGATAGGGAGCTGCACAGAGCTGCAAATGATAGACTTGAGCAACAATACTTTGGAAGGCCCCCTGCCTAACACATTATCATCACTATCAGGACTTCAAGTCTTGGATGTTTCAGTTAATCAGTTTTCGGAGCAGATACCAGCGAGCTTGGCTCGTCTTGTTTCGTTAAACAAGCTTATTCTGAGCAGGAACTTGTTCTCTGGATCGATACCAGCATCGCTTGGCCTGTGTTCAAGTCTCCAATTGCTTGATCTTAGTAGCAACAAGCTCACTGGCTCAATCCCCGTGGAGCTTGGGAGGATTGAAGCCCTTGAAATCGCATTAAATTTGAGCTGCAATGCACTGTCAGGCCCCATCCCACCTCAAATATCAGCACTCACCAAGCTTTCGATACTAGACCTTTCACATAACCAGCTTGATGGGGACTTGAGTCCACTTGCAGGGCTCGATAATCTTGTCTCTCTTAATGTGTCGCATAACAAACTCGGCGGCTATCTTCCAGACAACAAGCTTTTCAGACAGCTGTCACCAATGGATTTGGCTGGAAATGAAGGCCTTTGCTCTTCAAACCGCGACTCGTGTTTCCTTAGTGACGTTGGCCGAAAAGGACTTACAAGAAATCAAAATGAAATGAGGCGGTCACGCAGGCTCAAGCTAGCAATTGCATTGCTGATCACCCTGACAGTTGCAATGGTCGTTATGGGGATCGTTGCAGTGATTAGAGCGCAAAGAGCTATCAGAGATGATGACGATTCAGAACTGGGGAACTCATGGACTTGGCAGTTCACTCCATTCCAGAAGCTAAATTTCTCGGTTGAGCAAGTGCTAAGATGCCTGGTGGATGTCAATGTAATTGGAAAAGGGTGTTCTGGGGTTGTGTACCGTGCTGATATGGACAATGGGGAAGTCATTGCAGTGAAGAAGCTCTGGCCAACAACAGTTGCTGCAGACAATGGATGTTGTAATGATGAAAAATGTGGAGTTCGTGATTCATTCTCAGCAGAGGTCAAAACACTTGGCTCAATCCGTCACAAGAACATTGTTCGATTCCTGGGTTGTTGCTGGAACAAGAACACAAGATTGCTCATGTATGATTACATGCCTAATGGAAGCTTGGGAAGTATTCTGCATGAGAGGACAGGGCATGCCTTCGAATGGGAAGTTAGGTACCAAATTTTGTTGGGTGCAGCTCAAGGCATTGCCTATTTGCACCATGATTGTGTTCCTCCAATTGTTCACAGGGATATCAAGGCCAATAACATCCTCATTGGCCTTGAGTTTGAGCCTTACATTGCTGATTTTGGCCTAGCCAAACTTGTTGATGATGGCGATTTCGCTCGGTCCTCCAACACAGTTGCTGGTTCCTATGGCTATATTGCTCCTGAATATGGATACATGATGAAGATCACTGAAAAAAGCGATGTTTATAGCTATGGAGTTGTTGTATTGGAAGTCTTGACAGGGAAGCAACCAATCGATCCAACAATACCAGAGGGGCTACATGTAGTGGATTGGGTAAGACAGAAGAGGGGAAGCATTGAAGTCCTTGACCCAAGCCTCCTATCCAGACCCGAATCGGAGATAGGGGAAATGAGGCAGGCATTAGGCATAGCCTTGTTGTGTGTAAACTCAACACCTGATGAAAGACCAACAATGAAAGATGTGGCAGCAATGCTAAAAGAAATCAAACATGAGAGGGAGGAGTATGCCAAGGTTGATATGCTCCTCAAAGATCGATCACCGGCGAATGACGCGCGCGAAAATAAGAACCCTAATGGAGTTTTGGCAACCTCATCATCAGCAGCAGCAATGAAAAGCTTGTTTGCAAAAAGCAACGACACAAGTTTTTCTGCATCCACATTGCTTTACTCGTCTTCCTCCTCTAGTGCCAATAAAATGGGTTTCAAGTGA

Protein Analysis

820

Amino Acids

88.81

Weight (kDa)

5.61

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 118 - 177 7.5e-07 Leucine rich repeat
LRR_14 PF23598 141 - 363 8e-14 Leucine-rich repeat region
Pkinase PF00069 465 - 739 4.7e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 466 - 741 2.8e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24240
fragaria_vesca FvH4_4g05730
malus_domestica MD00G1164700.v1.1 MD16G1236300.v1.1
prunus_persica Prupe.1G060700_v2.0.a1
pyrus_communis pycom13g20340
rosa_chinensis RchiOBHm_Chr4g0396901
rosa_laevigata RLG00000009492
rosa_multiflora Rmu_sc0008929.1_g000009
rosa_roxburghii Rroxscaffold_5G00341810
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4BG071300 Rh4CG079500 Rh4DG068900
rosa_wichuraiana Rw4G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
Acc65I GGTACC 1 cut(s) 1716
AccB1I GGYRCC 1 cut(s) 1716
AccB7I CCANNNNNTGG 1 cut(s) 410
AccII CGCG 4 cut(s) 1122, 2303, 2305, 2307
AciI CCGC 4 cut(s) 439, 1038, 1120, 1186
AclWI GGATC 4 cut(s) 103, 760, 1262, 2021
AcoI YGGCCR 2 cut(s) 1147, 1478
AcsI RAATTY 4 cut(s) 19, 874, 1354, 1722
AfaI GTAC 2 cut(s) 1430, 1718
AfiI CCNNNNNNNGG 6 cut(s) 194, 361, 410, 989, 1704, 1937
AflIII ACRYGT 1 cut(s) 2050
AgeI ACCGGT 1 cut(s) 101
AhdI GACNNNNNGTC 1 cut(s) 647
AjnI CCWGG 2 cut(s) 1383, 1599
Alw26I GTCTC 4 cut(s) 287, 480, 791, 1012
AlwI GGATC 4 cut(s) 103, 760, 1262, 2021
AlwNI CAGNNNCTG 2 cut(s) 1493, 1907
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 9 cut(s) 607, 772, 898, 1102, 1147, 1478, 1800, 1819, 1852
ApoI RAATTY 4 cut(s) 19, 874, 1354, 1722
AsiGI ACCGGT 1 cut(s) 101
Asp700I GAANNNNTTC 1 cut(s) 743
Asp718I GGTACC 1 cut(s) 1716
AspLEI GCGC 3 cut(s) 1276, 2305, 2307
AspS9I GGNCC 3 cut(s) 608, 899, 1890
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 5 cut(s) 91, 919, 1068, 1216, 2280
AsuII TTCGAA 2 cut(s) 377, 1701
AsuNHI GCTAGC 1 cut(s) 1204
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 1 cut(s) 1890
AxyI CCTNAGG 1 cut(s) 491
BalI TGGCCA 1 cut(s) 1480
BanI GGYRCC 1 cut(s) 1716
BanII GRGCYC 1 cut(s) 996
BarI GAAGNNNNNNTAC 2 cut(s) 241, 273
BauI CACGAG 1 cut(s) 1126
BbsI GAAGAC 1 cut(s) 2415
BccI CCATC 3 cut(s) 911, 962, 1868
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 2 cut(s) 1385, 1601
BciVI GTATCC 1 cut(s) 1934
BclI TGATCA 1 cut(s) 1221
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 4 cut(s) 287, 480, 791, 1012
BfaI CTAG 6 cut(s) 270, 293, 944, 1205, 1856, 2433
BfmI CTRYAG 3 cut(s) 149, 418, 1494
BfuI GTATCC 1 cut(s) 1934
BglII AGATCT 1 cut(s) 168
Bme1390I CCNGG 3 cut(s) 520, 1385, 1601
Bme18I GGWCC 1 cut(s) 1890
BmeRI GACNNNNNGTC 1 cut(s) 647
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 3 cut(s) 608, 899, 1890
BmiI GGNNCC 5 cut(s) 524, 610, 901, 1718, 1912
BmrFI CCNGG 3 cut(s) 520, 1385, 1601
BmrI ACTGGG 1 cut(s) 1319
BmsI GCATC 4 cut(s) 132, 773, 1370, 2411
BmtI GCTAGC 1 cut(s) 1208
BmuI ACTGGG 1 cut(s) 1319
BoxI GACNNNNGTC 2 cut(s) 978, 1072
BpiI GAAGAC 1 cut(s) 2415
BplI GAGNNNNNCTC 2 cut(s) 685, 717
Bpu14I TTCGAA 2 cut(s) 377, 1701
BpuEI CTTGAG 6 cut(s) 89, 607, 997, 1184, 1725, 1844
BpuMI CCSGG 1 cut(s) 520
Bsa29I ATCGAT 3 cut(s) 755, 2025, 2284
BsaBI GATNNNNATC 1 cut(s) 1952
BsaI GGTCTC 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 834, 1600, 2259
BsaWI WCCGGW 3 cut(s) 101, 173, 187
BsaXI ACNNNNNCTCC 2 cut(s) 1977, 2007
Bsc4I CCNNNNNNNGG 6 cut(s) 194, 361, 410, 989, 1704, 1937
Bse118I RCCGGY 3 cut(s) 101, 332, 2290
Bse1I ACTGG 2 cut(s) 826, 1314
Bse21I CCTNAGG 1 cut(s) 491
Bse3DI GCAATG 9 cut(s) 892, 1214, 1245, 1458, 1746, 1839, 2226, 2364, 2408
Bse8I GATNNNNATC 1 cut(s) 1952
BseBI CCWGG 2 cut(s) 1385, 1601
BseCI ATCGAT 3 cut(s) 755, 2025, 2284
BseDI CCNNGG 3 cut(s) 834, 1600, 2259
BseGI GGATG 7 cut(s) 204, 661, 903, 1396, 1511, 1809, 2402
BseJI GATNNNNATC 1 cut(s) 1952
BseLI CCNNNNNNNGG 6 cut(s) 194, 361, 410, 989, 1704, 1937
BseMI GCAATG 9 cut(s) 892, 1214, 1245, 1458, 1746, 1839, 2226, 2364, 2408
BseMII CTCAG 2 cut(s) 722, 1560
BseNI ACTGG 2 cut(s) 826, 1314
BsePI GCGCGC 1 cut(s) 2303
BseRI GAGGAG 4 cut(s) 86, 2264, 2264, 2419
BsgI GTGCAG 2 cut(s) 547, 1755
Bsh1236I CGCG 4 cut(s) 1122, 2303, 2305, 2307
BshFI GGCC 9 cut(s) 609, 774, 900, 1104, 1149, 1480, 1802, 1821, 1854
BshNI GGYRCC 1 cut(s) 1716
BshTI ACCGGT 1 cut(s) 101
BshVI ATCGAT 3 cut(s) 755, 2025, 2284
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 6 cut(s) 102, 174, 188, 333, 519, 2291
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 6 cut(s) 194, 361, 410, 989, 1704, 1937
BsmAI GTCTC 4 cut(s) 287, 480, 791, 1012
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 2 cut(s) 252, 324
BsnI GGCC 9 cut(s) 609, 774, 900, 1104, 1149, 1480, 1802, 1821, 1854
Bso31I GGTCTC 1 cut(s) 480
BsoBI CYCGRG 1 cut(s) 388
Bsp119I TTCGAA 2 cut(s) 377, 1701
Bsp1286I GDGCHC 1 cut(s) 996
BspACI CCGC 4 cut(s) 439, 1038, 1120, 1186
BspANI GGCC 9 cut(s) 609, 774, 900, 1104, 1149, 1480, 1802, 1821, 1854
BspCNI CTCAG 2 cut(s) 723, 1559
BspDI ATCGAT 3 cut(s) 755, 2025, 2284
BspFNI CGCG 4 cut(s) 1122, 2303, 2305, 2307
BspLI GGNNCC 5 cut(s) 524, 610, 901, 1718, 1912
BspMAI CTGCAG 3 cut(s) 153, 422, 1498
BspOI GCTAGC 1 cut(s) 1208
BspPI GGATC 4 cut(s) 103, 760, 1262, 2021
BspQI GCTCTTC 1 cut(s) 1117
BspT104I TTCGAA 2 cut(s) 377, 1701
BspT107I GGYRCC 1 cut(s) 1716
BspTNI GGTCTC 1 cut(s) 480
BsrDI GCAATG 9 cut(s) 892, 1214, 1245, 1458, 1746, 1839, 2226, 2364, 2408
BsrFI RCCGGY 3 cut(s) 101, 332, 2290
BsrI ACTGG 2 cut(s) 826, 1314
BssAI RCCGGY 3 cut(s) 101, 332, 2290
BssECI CCNNGG 3 cut(s) 834, 1600, 2259
BssHII GCGCGC 1 cut(s) 2303
BssSI CACGAG 1 cut(s) 1126
BssT1I CCWWGG 1 cut(s) 2259
Bst2BI CACGAG 1 cut(s) 1126
Bst2UI CCWGG 2 cut(s) 1385, 1601
Bst4CI ACNGT 5 cut(s) 894, 1234, 1433, 1489, 1903
Bst6I CTCTTC 2 cut(s) 1117, 2069
BstBI TTCGAA 2 cut(s) 377, 1701
BstC8I GCNNGC 7 cut(s) 334, 694, 1196, 1206, 1695, 2148, 2305
BstDEI CTNAG 6 cut(s) 491, 731, 803, 1136, 1376, 1546
BstDSI CCRYGG 1 cut(s) 834
BstENI CCTNNNNNAGG 1 cut(s) 359
BstF5I GGATG 7 cut(s) 204, 661, 903, 1396, 1511, 1809, 2402
BstFNI CGCG 4 cut(s) 1122, 2303, 2305, 2307
BstHHI GCGC 3 cut(s) 1276, 2305, 2307
BstMAI GTCTC 4 cut(s) 287, 480, 791, 1012
BstMWI GCNNNNNNNGC 5 cut(s) 151, 238, 698, 1280, 2300
BstNI CCWGG 2 cut(s) 1385, 1601
BstNSI RCATGY 3 cut(s) 1651, 1697, 2054
BstPAI GACNNNNGTC 2 cut(s) 978, 1072
BstSCI CCNGG 3 cut(s) 518, 1383, 1599
BstSFI CTRYAG 3 cut(s) 149, 418, 1494
BstUI CGCG 4 cut(s) 1122, 2303, 2305, 2307
BstV2I GAAGAC 1 cut(s) 2415
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 3 cut(s) 533, 968, 2446
BstYI RGATCY 1 cut(s) 168
Bsu15I ATCGAT 3 cut(s) 755, 2025, 2284
Bsu36I CCTNAGG 1 cut(s) 491
BsuI GTATCC 1 cut(s) 1934
BsuRI GGCC 9 cut(s) 609, 774, 900, 1104, 1149, 1480, 1802, 1821, 1854
BsuTUI ATCGAT 3 cut(s) 755, 2025, 2284
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 2 cut(s) 750, 1983
BtsCI GGATG 7 cut(s) 204, 661, 903, 1396, 1511, 1809, 2402
BtsI GCAGTG 2 cut(s) 1269, 1470
BtsIMutI CAGTG 5 cut(s) 819, 890, 1269, 1470, 1956
Cac8I GCNNGC 7 cut(s) 334, 694, 1196, 1206, 1695, 2148, 2305
CaiI CAGNNNCTG 2 cut(s) 1493, 1907
CfoI GCGC 3 cut(s) 1276, 2305, 2307
Cfr10I RCCGGY 3 cut(s) 101, 332, 2290
Cfr13I GGNCC 3 cut(s) 608, 899, 1890
ClaI ATCGAT 3 cut(s) 755, 2025, 2284
CseI GACGC 1 cut(s) 2309
Csp6I GTAC 2 cut(s) 1429, 1717
CspAI ACCGGT 1 cut(s) 101
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 9 cut(s) 1320, 1636, 1648, 1679, 1694, 1763, 1945, 2051, 2240
CviQI GTAC 2 cut(s) 1429, 1717
DdeI CTNAG 6 cut(s) 491, 731, 803, 1136, 1376, 1546
DrdI GACNNNNNNGTC 1 cut(s) 95
DriI GACNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 2 cut(s) 1147, 1478
Eam1104I CTCTTC 2 cut(s) 1117, 2069
Eam1105I GACNNNNNGTC 1 cut(s) 647
EarI CTCTTC 2 cut(s) 1117, 2069
Eco130I CCWWGG 1 cut(s) 2259
Eco147I AGGCCT 1 cut(s) 1104
Eco24I GRGCYC 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 480
Eco32I GATATC 1 cut(s) 1795
Eco47I GGWCC 1 cut(s) 1890
Eco81I CCTNAGG 1 cut(s) 491
Eco88I CYCGRG 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 359
EcoO109I RGGNCCY 2 cut(s) 608, 899
EcoRII CCWGG 2 cut(s) 1383, 1599
EcoRV GATATC 1 cut(s) 1795
EcoT14I CCWWGG 1 cut(s) 2259
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 1 cut(s) 2259
FaeI CATG 9 cut(s) 1323, 1639, 1651, 1682, 1697, 1766, 1948, 2054, 2243
FaqI GGGAC 1 cut(s) 986
FatI CATG 9 cut(s) 1319, 1635, 1647, 1678, 1693, 1762, 1944, 2050, 2239
FbaI TGATCA 1 cut(s) 1221
FokI GGATG 7 cut(s) 211, 668, 890, 1403, 1518, 1796, 2389
FriOI GRGCYC 1 cut(s) 996
FspBI CTAG 6 cut(s) 270, 293, 944, 1205, 1856, 2433
GlaI GCGC 3 cut(s) 1275, 2304, 2306
HaeIII GGCC 9 cut(s) 609, 774, 900, 1104, 1149, 1480, 1802, 1821, 1854
HapII CCGG 6 cut(s) 102, 174, 188, 333, 519, 2291
HgaI GACGC 1 cut(s) 2309
HhaI GCGC 3 cut(s) 1276, 2305, 2307
Hin1II CATG 9 cut(s) 1323, 1639, 1651, 1682, 1697, 1766, 1948, 2054, 2243
Hin6I GCGC 3 cut(s) 1274, 2303, 2305
HinP1I GCGC 3 cut(s) 1274, 2303, 2305
HindIII AAGCTT 5 cut(s) 722, 932, 1058, 1660, 2365
HinfI GANTC 9 cut(s) 64, 181, 469, 979, 1124, 1301, 1538, 1596, 2123
HpaII CCGG 6 cut(s) 102, 174, 188, 333, 519, 2291
HphI GGTGA 5 cut(s) 91, 919, 1068, 1216, 2280
Hpy166II GTNNAC 5 cut(s) 983, 1335, 1429, 1786, 2176
Hpy188I TCNGA 8 cut(s) 168, 474, 679, 732, 1067, 1289, 1306, 2128
Hpy8I GTNNAC 5 cut(s) 983, 1335, 1429, 1786, 2176
HpyAV CCTTC 6 cut(s) 123, 239, 267, 599, 1094, 1708
HpyCH4III ACNGT 5 cut(s) 894, 1234, 1433, 1489, 1903
HpyCH4IV ACGT 1 cut(s) 1143
HpyF10VI GCNNNNNNNGC 5 cut(s) 151, 238, 698, 1280, 2300
HpyF3I CTNAG 6 cut(s) 491, 731, 803, 1136, 1376, 1546
HpySE526I ACGT 1 cut(s) 1143
Hsp92II CATG 9 cut(s) 1323, 1639, 1651, 1682, 1697, 1766, 1948, 2054, 2243
HspAI GCGC 3 cut(s) 1274, 2303, 2305
KpnI GGTACC 1 cut(s) 1720
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 1221
LguI GCTCTTC 1 cut(s) 1117
LmnI GCTCC 6 cut(s) 495, 558, 679, 838, 1933, 2277
LweI GCATC 4 cut(s) 132, 773, 1370, 2411
MaeI CTAG 6 cut(s) 270, 293, 944, 1205, 1856, 2433
MaeII ACGT 1 cut(s) 1143
MaeIII GTNAC 8 cut(s) 86, 97, 300, 413, 1074, 1139, 1188, 1577
MauBI CGCGCGCG 1 cut(s) 2303
MboII GAAGA 6 cut(s) 1037, 1104, 1480, 1963, 2086, 2415
MfeI CAATTG 4 cut(s) 480, 791, 1209, 1779
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 996
MlsI TGGCCA 1 cut(s) 1480
MluNI TGGCCA 1 cut(s) 1480
MlyI GAGTC 2 cut(s) 988, 1118
MmeI TCCRAC 2 cut(s) 1920, 2054
Mox20I TGGCCA 1 cut(s) 1480
MroNI GCCGGC 1 cut(s) 332
MroXI GAANNNNTTC 1 cut(s) 743
MscI TGGCCA 1 cut(s) 1480
MseI TTAA 5 cut(s) 500, 666, 716, 872, 1014
MslI CAYNNNNRTG 2 cut(s) 1463, 1767
Msp20I TGGCCA 1 cut(s) 1480
MspA1I CMGCKG 2 cut(s) 148, 1072
MspI CCGG 6 cut(s) 102, 174, 188, 333, 519, 2291
MspR9I CCNGG 3 cut(s) 520, 1385, 1601
MunI CAATTG 4 cut(s) 480, 791, 1209, 1779
Mva1269I GAATGC 2 cut(s) 252, 324
MvaI CCWGG 2 cut(s) 1385, 1601
MvnI CGCG 4 cut(s) 1122, 2303, 2305, 2307
MwoI GCNNNNNNNGC 5 cut(s) 151, 238, 698, 1280, 2300
NaeI GCCGGC 1 cut(s) 334
NciI CCSGG 1 cut(s) 520
NgoMIV GCCGGC 1 cut(s) 332
NheI GCTAGC 1 cut(s) 1204
NlaIII CATG 9 cut(s) 1323, 1639, 1651, 1682, 1697, 1766, 1948, 2054, 2243
NlaIV GGNNCC 5 cut(s) 524, 610, 901, 1718, 1912
NmeAIII GCCGAG 1 cut(s) 1014
NmuCI GTSAC 7 cut(s) 86, 97, 300, 1074, 1139, 1188, 1577
NspI RCATGY 3 cut(s) 1651, 1697, 2054
NspV TTCGAA 2 cut(s) 377, 1701
PaeI GCATGC 1 cut(s) 1697
PauI GCGCGC 1 cut(s) 2303
PceI AGGCCT 1 cut(s) 1104
PciI ACATGT 1 cut(s) 2050
PciSI GCTCTTC 1 cut(s) 1117
PctI GAATGC 2 cut(s) 252, 324
PdiI GCCGGC 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 743
PfeI GAWTC 7 cut(s) 64, 181, 469, 1301, 1538, 1596, 2123
PflMI CCANNNNNTGG 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 518
PinAI ACCGGT 1 cut(s) 101
PleI GAGTC 2 cut(s) 987, 1118
PpsI GAGTC 2 cut(s) 987, 1118
PscI ACATGT 1 cut(s) 2050
PshAI GACNNNNGTC 2 cut(s) 978, 1072
Psp6I CCWGG 2 cut(s) 1383, 1599
PspGI CCWGG 2 cut(s) 1383, 1599
PspN4I GGNNCC 5 cut(s) 524, 610, 901, 1718, 1912
PspPI GGNCC 3 cut(s) 608, 899, 1890
PstI CTGCAG 3 cut(s) 153, 422, 1498
PstNI CAGNNNCTG 2 cut(s) 1493, 1907
PsuI RGATCY 1 cut(s) 168
PteI GCGCGC 1 cut(s) 2303
PvuII CAGCTG 2 cut(s) 148, 1072
RsaI GTAC 2 cut(s) 1430, 1718
RsaNI GTAC 2 cut(s) 1429, 1717
RseI CAYNNNNRTG 2 cut(s) 1463, 1767
SapI GCTCTTC 1 cut(s) 1117
SaqAI TTAA 5 cut(s) 500, 666, 716, 872, 1014
Sau96I GGNCC 3 cut(s) 608, 899, 1890
SchI GAGTC 2 cut(s) 988, 1118
ScrFI CCNGG 3 cut(s) 520, 1385, 1601
SduI GDGCHC 1 cut(s) 996
SfaNI GCATC 4 cut(s) 132, 773, 1370, 2411
SfcI CTRYAG 3 cut(s) 149, 418, 1494
SfuI TTCGAA 2 cut(s) 377, 1701
SgrAI CRCCGGYG 1 cut(s) 2290
SinI GGWCC 1 cut(s) 1890
SmiMI CAYNNNNRTG 2 cut(s) 1463, 1767
SmlI CTYRAG 6 cut(s) 68, 586, 976, 1199, 1740, 1823
SmoI CTYRAG 6 cut(s) 68, 586, 976, 1199, 1740, 1823
SphI GCATGC 1 cut(s) 1697
SseBI AGGCCT 1 cut(s) 1104
SsiI CCGC 4 cut(s) 439, 1038, 1120, 1186
SspMI CTAG 6 cut(s) 270, 293, 944, 1205, 1856, 2433
StuI AGGCCT 1 cut(s) 1104
StyD4I CCNGG 3 cut(s) 518, 1383, 1599
StyI CCWWGG 1 cut(s) 2259
TaaI ACNGT 5 cut(s) 894, 1234, 1433, 1489, 1903
TaiI ACGT 1 cut(s) 1146
TaqII GACCGA 1 cut(s) 1878
TauI GCSGC 2 cut(s) 442, 1041
TfiI GAWTC 7 cut(s) 64, 181, 469, 1301, 1538, 1596, 2123
Tru1I TTAA 5 cut(s) 500, 666, 716, 872, 1014
Tru9I TTAA 5 cut(s) 500, 666, 716, 872, 1014
TscAI CASTG 5 cut(s) 826, 897, 1269, 1470, 1963
TseFI GTSAC 7 cut(s) 86, 97, 300, 1074, 1139, 1188, 1577
Tsp45I GTSAC 7 cut(s) 86, 97, 300, 1074, 1139, 1188, 1577
TspDTI ATGAA 9 cut(s) 385, 1113, 1191, 1530, 1533, 1964, 2205, 2219, 2375
TspGWI ACGGA 1 cut(s) 1565
TspRI CASTG 5 cut(s) 826, 897, 1269, 1470, 1963
Van91I CCANNNNNTGG 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 1890
XagI CCTNNNNNAGG 1 cut(s) 359
XapI RAATTY 4 cut(s) 19, 874, 1354, 1722
XceI RCATGY 3 cut(s) 1651, 1697, 2054
XmnI GAANNNNTTC 1 cut(s) 743
XspI CTAG 6 cut(s) 270, 293, 944, 1205, 1856, 2433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.