MD00G1204600.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004640
Physical Location & Seq
Reverse (-)
49576343 .. 49578653
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1204600.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGGAAACAAAAATTGATGATAGCTTATGGGTTTTGGCAATCTTCTCCAAATGCCAAGCCTTAACCTCCACAACCGCCGTCGTTGCATTCCTCTGCACGGCCGCAGCTTGGCTAAGCGCGGCCATCTTCTACTGGGCATCTTCTCCCGGAGGCGCAGCATGGGGAAAACTTCGATGGAGGAAAAGAAACTCTTGTAATAAAAATGAAATCACCAACTCCAACCCGATTCCCGGGCCACGTGGATTTCCAATAATAGGAAGCATGAACCTCATGACAAACCTAGCTCACCACAAACTCACAAAAGCGGCGGAGGTTTATGGAGCAAAGAGGCTCATGGCTTTTAGCATAGGCGAGACGCGCGCCATCGTCACATGCGACCCCGACGTAGCTAAGGAAATCCTCAACAGCTCCGTCTTTGCCAACCGTCCTATAAAAGAGTCGGCTTACTGTCTCATGTTCAACCGAGCAATCGGGTTCGCTCCTTATGGAAGCTACTGGCGTACCCTCCGCCGCATCGCTGCCAACCATCTCTTCTGCCCGAAGCAAATCTCCACTTCCGAGTATCTCCGTTCCAGGATTGCTGATCAAATGGTGTCCCTTATCTCGACAAGTTGTAACAACAGCTTCCTTGTCCGCGACGTACTTAGGAAGGCTTCGTTGAACAGCATGATGTGCTCGGTTTTTGGACGTAAATACGAGCTGAGTTCGTCAAATGAAGAGACTGAGGAACTGAATCGACTTGTTGATGAAGGTTATGATCTGTTGGGAAATTTGAACTGGTCTGATCACCTGCCTTGGCTTGCCGGTCTGGACCTCCAGAGCATCCGGTTTAGATGCAACAAACTCGTTCCTCAAGTGAACCGGCTCGTCAGCCGAATTATTCAAGAGCATAGAGCCCAAACCGGTCCTAATAACAATGATTTTGTCGACGTTCTTCTATCTCTTGATGGAGCTGATAAGCTGTGTGACAATGATATGATTTCAGTTCTTTGGGAGATGATATTCCGAGGAACAGATACTGTAGCGGTTCTGATAGAGTGGATCCTTGCAAGGATGGTGATGCATCATGATATCCAATCAAAGGTTCATGAGGAGCTCGACCGCGTTGTGGGAAAATCAAGGGCGTTGCTGGAATCCGACATTCAATCCTTGCCTTATCTGCAAGCAATGGTCAAAGAAGTGCTCAGAATGCATCCTCCGGGCCCACTCCTATCATGGGCCCGTTTAGCCATCACAGATACTGTAGTGGATGGCTATTACGTGCCAGCAGGGACCACAGCAATGGTGAATATGTGGGCCATAACAAGGGAACCAAAAGTGTGGTCGGATCCACTGGAGTTTCGGCCAGAGAGATTTGTATCTACTAAGAAGAAAGCTGACTTGCAGTTCTCTGTGTTCGGGTCTGACTTGAGGTTGGCACCGTTTGGGTCGGGCAGGAGGAGCTGCCCGGGGAAGACATTGGGGTTGAGTACAGTCAACTACTGGGTCGGGTTGTTGTTACATGAGTTTGAGTGGTTTAAGTCGGATCTTGATGCCAAGGTAGACCTGTCCGAAGTGATGAGGCTTTCATGCCAAATGGCTAACCCTCTCACCATCCAAGTGCATAATAGGCGCTACTAA

Protein Analysis

540

Amino Acids

60.83

Weight (kDa)

8.81

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 77 - 515 1.6e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 798
Acc36I ACCTGC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 1417
AccI GTMKAC 2 cut(s) 927, 1542
AccII CGCG 5 cut(s) 119, 358, 360, 636, 1104
AclWI GGATC 5 cut(s) 1036, 1049, 1322, 1335, 1533
AcoI YGGCCR 3 cut(s) 99, 120, 1343
AcsI RAATTY 1 cut(s) 769
AcvI CACGTG 1 cut(s) 239
AfaI GTAC 3 cut(s) 502, 642, 1471
AfiI CCNNNNNNNGG 8 cut(s) 97, 108, 230, 254, 1081, 1108, 1216, 1305
AgeI ACCGGT 1 cut(s) 902
AgsI TTSAA 5 cut(s) 460, 661, 775, 884, 1145
AjnI CCWGG 1 cut(s) 572
AjuI GAANNNNNNNTTGG 4 cut(s) 515, 547, 1068, 1100
Alw21I GWGCWC 3 cut(s) 677, 1098, 1185
Alw26I GTCTC 3 cut(s) 347, 455, 713
AlwI GGATC 5 cut(s) 1036, 1049, 1322, 1335, 1533
AlwNI CAGNNNCTG 2 cut(s) 1019, 1241
Ama87I CYCGRG 2 cut(s) 230, 1447
AoxI GGCC 7 cut(s) 99, 120, 233, 1201, 1218, 1296, 1343
ApaI GGGCCC 2 cut(s) 1205, 1222
ApeKI GCWGC 4 cut(s) 104, 155, 518, 1443
ApoI RAATTY 1 cut(s) 769
ArsI GACNNNNNNTTYG 2 cut(s) 1307, 1339
AsiGI ACCGGT 1 cut(s) 902
AspLEI GCGC 5 cut(s) 119, 155, 360, 362, 1614
AspS9I GGNCC 9 cut(s) 233, 811, 905, 1201, 1202, 1218, 1219, 1272, 1296
AsuC2I CCSGG 6 cut(s) 147, 231, 232, 1200, 1448, 1449
AsuHPI GGTGA 6 cut(s) 202, 278, 779, 1069, 1297, 1582
AvaI CYCGRG 2 cut(s) 230, 1447
AvaII GGWCC 3 cut(s) 811, 905, 1272
BaeGI GKGCMC 2 cut(s) 1205, 1222
BaeI ACNNNNGTAYC 4 cut(s) 484, 517, 1230, 1263
BamHI GGATCC 2 cut(s) 1041, 1327
BanI GGYRCC 1 cut(s) 1417
BanII GRGCYC 4 cut(s) 898, 1098, 1205, 1222
BbrPI CACGTG 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 1460
Bbv12I GWGCWC 3 cut(s) 677, 1098, 1185
BbvI GCAGC 4 cut(s) 116, 167, 505, 1430
BccI CCATC 9 cut(s) 131, 168, 371, 534, 941, 1048, 1238, 1244, 1601
BceAI ACGGC 2 cut(s) 62, 114
BcgI CGANNNNNNTGC 2 cut(s) 826, 860
BciT130I CCWGG 1 cut(s) 574
BclI TGATCA 2 cut(s) 583, 784
BcnI CCSGG 6 cut(s) 147, 231, 232, 1200, 1448, 1449
BcoDI GTCTC 3 cut(s) 347, 455, 713
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 2 cut(s) 1020, 1242
BfoI RGCGCY 1 cut(s) 1615
BfuAI ACCTGC 1 cut(s) 798
BisI GCNGC 8 cut(s) 102, 105, 120, 156, 306, 511, 519, 1444
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 8 cut(s) 103, 106, 121, 157, 307, 512, 520, 1445
Bme1390I CCNGG 7 cut(s) 147, 231, 232, 574, 1200, 1448, 1449
Bme18I GGWCC 3 cut(s) 811, 905, 1272
BmeT110I CYCGRG 2 cut(s) 230, 1447
BmgT120I GGNCC 9 cut(s) 233, 811, 905, 1201, 1202, 1218, 1219, 1272, 1296
BmiI GGNNCC 7 cut(s) 1043, 1203, 1220, 1273, 1311, 1329, 1419
BmrFI CCNGG 7 cut(s) 147, 231, 232, 574, 1200, 1448, 1449
BmrI ACTGGG 2 cut(s) 142, 1492
BmsI GCATC 8 cut(s) 146, 522, 824, 831, 1050, 1072, 1201, 1522
BmuI ACTGGG 2 cut(s) 142, 1492
BpiI GAAGAC 1 cut(s) 1460
BpmI CTGGAG 2 cut(s) 800, 1355
Bpu10I CCTNAGC 1 cut(s) 390
Bpu1102I GCTNAGC 1 cut(s) 113
BpuEI CTTGAG 2 cut(s) 837, 1429
BpuMI CCSGG 6 cut(s) 147, 231, 232, 1200, 1448, 1449
BsaAI YACGTR 2 cut(s) 239, 1261
BsaBI GATNNNNATC 1 cut(s) 1357
BsaJI CCNNGG 6 cut(s) 230, 794, 1006, 1447, 1448, 1536
BsaWI WCCGGW 2 cut(s) 825, 902
Bsc4I CCNNNNNNNGG 8 cut(s) 97, 108, 230, 254, 1081, 1108, 1216, 1305
Bse118I RCCGGY 3 cut(s) 803, 861, 902
Bse1I ACTGG 5 cut(s) 137, 500, 782, 1338, 1487
Bse3DI GCAATG 2 cut(s) 1173, 1287
Bse8I GATNNNNATC 1 cut(s) 1357
BseBI CCWGG 1 cut(s) 574
BseDI CCNNGG 6 cut(s) 230, 794, 1006, 1447, 1448, 1536
BseGI GGATG 5 cut(s) 822, 1059, 1192, 1255, 1593
BseJI GATNNNNATC 1 cut(s) 1357
BseLI CCNNNNNNNGG 8 cut(s) 97, 108, 230, 254, 1081, 1108, 1216, 1305
BseMI GCAATG 2 cut(s) 1173, 1287
BseMII CTCAG 3 cut(s) 692, 714, 1198
BseNI ACTGG 5 cut(s) 137, 500, 782, 1338, 1487
BsePI GCGCGC 1 cut(s) 358
BseRI GAGGAG 2 cut(s) 1106, 1453
BseSI GKGCMC 2 cut(s) 1205, 1222
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 4 cut(s) 116, 167, 505, 1430
BsgI GTGCAG 1 cut(s) 79
Bsh1236I CGCG 5 cut(s) 119, 358, 360, 636, 1104
Bsh1285I CGRYCG 2 cut(s) 102, 1102
BshFI GGCC 7 cut(s) 101, 122, 235, 1203, 1220, 1298, 1345
BshNI GGYRCC 1 cut(s) 1417
BshTI ACCGGT 1 cut(s) 902
BsiEI CGRYCG 2 cut(s) 102, 1102
BsiHKAI GWGCWC 3 cut(s) 677, 1098, 1185
BsiHKCI CYCGRG 2 cut(s) 230, 1447
BsiSI CCGG 8 cut(s) 147, 231, 804, 826, 862, 903, 1199, 1448
BslFI GGGAC 2 cut(s) 580, 1285
BslI CCNNNNNNNGG 8 cut(s) 97, 108, 230, 254, 1081, 1108, 1216, 1305
BsmAI GTCTC 3 cut(s) 347, 455, 713
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 2 cut(s) 580, 1285
BsmI GAATGC 2 cut(s) 86, 1194
BsnI GGCC 7 cut(s) 101, 122, 235, 1203, 1220, 1298, 1345
BsoBI CYCGRG 2 cut(s) 230, 1447
Bsp120I GGGCCC 2 cut(s) 1201, 1218
Bsp1286I GDGCHC 6 cut(s) 677, 898, 1098, 1185, 1205, 1222
Bsp143I GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
Bsp1720I GCTNAGC 1 cut(s) 113
BspANI GGCC 7 cut(s) 101, 122, 235, 1203, 1220, 1298, 1345
BspCNI CTCAG 3 cut(s) 693, 715, 1197
BspFNI CGCG 5 cut(s) 119, 358, 360, 636, 1104
BspHI TCATGA 3 cut(s) 270, 1066, 1087
BspLI GGNNCC 7 cut(s) 1043, 1203, 1220, 1273, 1311, 1329, 1419
BspMI ACCTGC 1 cut(s) 798
BspPI GGATC 5 cut(s) 1036, 1049, 1322, 1335, 1533
BspT107I GGYRCC 1 cut(s) 1417
BsrDI GCAATG 2 cut(s) 1173, 1287
BsrFI RCCGGY 3 cut(s) 803, 861, 902
BsrI ACTGG 5 cut(s) 137, 500, 782, 1338, 1487
BssAI RCCGGY 3 cut(s) 803, 861, 902
BssECI CCNNGG 6 cut(s) 230, 794, 1006, 1447, 1448, 1536
BssHII GCGCGC 1 cut(s) 358
BssMI GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
BssT1I CCWWGG 2 cut(s) 794, 1536
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 6 cut(s) 425, 449, 1021, 1243, 1422, 1474
Bst6I CTCTTC 2 cut(s) 536, 711
BstAPI GCANNNNNTGC 1 cut(s) 672
BstBAI YACGTR 2 cut(s) 239, 1261
BstC8I GCNNGC 4 cut(s) 360, 801, 1164, 1266
BstDEI CTNAG 7 cut(s) 113, 390, 644, 701, 723, 1184, 1365
BstF5I GGATG 5 cut(s) 822, 1059, 1192, 1255, 1593
BstFNI CGCG 5 cut(s) 119, 358, 360, 636, 1104
BstH2I RGCGCY 1 cut(s) 1615
BstHHI GCGC 5 cut(s) 119, 155, 360, 362, 1614
BstKTI GATC 6 cut(s) 586, 760, 787, 1044, 1330, 1528
BstMAI GTCTC 3 cut(s) 347, 455, 713
BstMBI GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
BstMCI CGRYCG 2 cut(s) 102, 1102
BstMWI GCNNNNNNNGC 8 cut(s) 83, 357, 672, 1159, 1189, 1226, 1440, 1609
BstNI CCWGG 1 cut(s) 574
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 7 cut(s) 145, 229, 230, 572, 1198, 1446, 1447
BstSFI CTRYAG 2 cut(s) 1020, 1242
BstSLI GKGCMC 2 cut(s) 1205, 1222
BstUI CGCG 5 cut(s) 119, 358, 360, 636, 1104
BstV1I GCAGC 4 cut(s) 116, 167, 505, 1430
BstV2I GAAGAC 1 cut(s) 1460
BstX2I RGATCY 3 cut(s) 1041, 1327, 1525
BstXI CCANNNNNNTGG 2 cut(s) 1282, 1320
BstYI RGATCY 3 cut(s) 1041, 1327, 1525
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 7 cut(s) 101, 122, 235, 1203, 1220, 1298, 1345
BtgZI GCGATG 1 cut(s) 499
BtsCI GGATG 5 cut(s) 822, 1059, 1192, 1255, 1593
BtsIMutI CAGTG 1 cut(s) 1331
BveI ACCTGC 1 cut(s) 798
Cac8I GCNNGC 4 cut(s) 360, 801, 1164, 1266
CaiI CAGNNNCTG 2 cut(s) 1019, 1241
CciI TCATGA 3 cut(s) 270, 1066, 1087
CfoI GCGC 5 cut(s) 119, 155, 360, 362, 1614
Cfr10I RCCGGY 3 cut(s) 803, 861, 902
Cfr13I GGNCC 9 cut(s) 233, 811, 905, 1201, 1202, 1218, 1219, 1272, 1296
Cfr9I CCCGGG 2 cut(s) 230, 1447
CseI GACGC 1 cut(s) 364
Csp6I GTAC 3 cut(s) 501, 641, 1470
CspAI ACCGGT 1 cut(s) 902
CviQI GTAC 3 cut(s) 501, 641, 1470
DdeI CTNAG 7 cut(s) 113, 390, 644, 701, 723, 1184, 1365
DpnI GATC 6 cut(s) 585, 759, 786, 1043, 1329, 1527
DpnII GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
EaeI YGGCCR 3 cut(s) 99, 120, 1343
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 2 cut(s) 536, 711
EarI CTCTTC 2 cut(s) 536, 711
EciI GGCGGA 2 cut(s) 323, 497
Ecl136II GAGCTC 1 cut(s) 1096
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 794, 1536
Eco24I GRGCYC 4 cut(s) 898, 1098, 1205, 1222
Eco32I GATATC 1 cut(s) 1072
Eco47I GGWCC 3 cut(s) 811, 905, 1272
Eco52I CGGCCG 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 1096
Eco72I CACGTG 1 cut(s) 239
Eco88I CYCGRG 2 cut(s) 230, 1447
EcoICRI GAGCTC 1 cut(s) 1096
EcoRII CCWGG 1 cut(s) 572
EcoRV GATATC 1 cut(s) 1072
EcoT14I CCWWGG 2 cut(s) 794, 1536
EcoT22I ATGCAT 2 cut(s) 1065, 1194
EcoT38I GRGCYC 4 cut(s) 898, 1098, 1205, 1222
ErhI CCWWGG 2 cut(s) 794, 1536
Esp3I CGTCTC 1 cut(s) 347
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 2 cut(s) 580, 1285
FbaI TGATCA 2 cut(s) 583, 784
FblI GTMKAC 2 cut(s) 927, 1542
Fnu4HI GCNGC 8 cut(s) 102, 105, 120, 156, 306, 511, 519, 1444
FokI GGATG 5 cut(s) 809, 1066, 1179, 1262, 1580
FriOI GRGCYC 4 cut(s) 898, 1098, 1205, 1222
Fsp4HI GCNGC 8 cut(s) 102, 105, 120, 156, 306, 511, 519, 1444
FspBI CTAG 1 cut(s) 281
GlaI GCGC 5 cut(s) 118, 154, 359, 361, 1613
GluI GCNGC 8 cut(s) 102, 105, 120, 156, 306, 511, 519, 1444
GsuI CTGGAG 2 cut(s) 800, 1355
HaeII RGCGCY 1 cut(s) 1615
HaeIII GGCC 7 cut(s) 101, 122, 235, 1203, 1220, 1298, 1345
HapII CCGG 8 cut(s) 147, 231, 804, 826, 862, 903, 1199, 1448
HgaI GACGC 1 cut(s) 364
HhaI GCGC 5 cut(s) 119, 155, 360, 362, 1614
Hin6I GCGC 5 cut(s) 117, 153, 358, 360, 1612
HinP1I GCGC 5 cut(s) 117, 153, 358, 360, 1612
HincII GTYRAC 2 cut(s) 928, 1477
HindII GTYRAC 2 cut(s) 928, 1477
HinfI GANTC 4 cut(s) 226, 437, 733, 1133
HpaII CCGG 8 cut(s) 147, 231, 804, 826, 862, 903, 1199, 1448
HphI GGTGA 6 cut(s) 202, 278, 779, 1069, 1297, 1582
Hpy166II GTNNAC 4 cut(s) 859, 928, 1477, 1543
Hpy188III TCNNGA 9 cut(s) 271, 604, 809, 817, 884, 944, 1067, 1088, 1529
Hpy8I GTNNAC 4 cut(s) 859, 928, 1477, 1543
Hpy99I CGWCG 4 cut(s) 83, 386, 641, 932
HpyAV CCTTC 2 cut(s) 643, 743
HpyCH4III ACNGT 6 cut(s) 425, 449, 1021, 1243, 1422, 1474
HpyCH4IV ACGT 6 cut(s) 238, 384, 639, 688, 930, 1260
HpyCH4V TGCA 9 cut(s) 86, 96, 837, 1049, 1063, 1162, 1192, 1384, 1603
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 357, 672, 1159, 1189, 1226, 1440, 1609
HpyF3I CTNAG 7 cut(s) 113, 390, 644, 701, 723, 1184, 1365
HpySE526I ACGT 6 cut(s) 238, 384, 639, 688, 930, 1260
HspAI GCGC 5 cut(s) 117, 153, 358, 360, 1612
Ksp22I TGATCA 2 cut(s) 583, 784
Kzo9I GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
LmnI GCTCC 6 cut(s) 320, 413, 484, 950, 1093, 1440
Lsp1109I GCAGC 4 cut(s) 116, 167, 505, 1430
LweI GCATC 8 cut(s) 146, 522, 824, 831, 1050, 1072, 1201, 1522
MaeI CTAG 1 cut(s) 281
MaeII ACGT 6 cut(s) 238, 384, 639, 688, 930, 1260
MaeIII GTNAC 4 cut(s) 367, 614, 965, 1497
MalI GATC 6 cut(s) 585, 759, 786, 1043, 1329, 1527
MboI GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
MboII GAAGA 8 cut(s) 34, 118, 132, 523, 728, 926, 1381, 1465
MflI RGATCY 3 cut(s) 1041, 1327, 1525
MhlI GDGCHC 6 cut(s) 677, 898, 1098, 1185, 1205, 1222
MluCI AATT 3 cut(s) 12, 769, 876
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 4 cut(s) 243, 1161, 1305, 1503
Mph1103I ATGCAT 2 cut(s) 1065, 1194
MseI TTAA 2 cut(s) 62, 1518
MslI CAYNNNNRTG 2 cut(s) 1280, 1598
MspI CCGG 8 cut(s) 147, 231, 804, 826, 862, 903, 1199, 1448
MspR9I CCNGG 7 cut(s) 147, 231, 232, 574, 1200, 1448, 1449
Mva1269I GAATGC 2 cut(s) 86, 1194
MvaI CCWGG 1 cut(s) 574
MvnI CGCG 5 cut(s) 119, 358, 360, 636, 1104
MwoI GCNNNNNNNGC 8 cut(s) 83, 357, 672, 1159, 1189, 1226, 1440, 1609
NciI CCSGG 6 cut(s) 147, 231, 232, 1200, 1448, 1449
NdeII GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
NlaIV GGNNCC 7 cut(s) 1043, 1203, 1220, 1273, 1311, 1329, 1419
NmuCI GTSAC 2 cut(s) 367, 965
NsiI ATGCAT 2 cut(s) 1065, 1194
NspI RCATGY 1 cut(s) 375
PagI TCATGA 3 cut(s) 270, 1066, 1087
PaqCI CACCTGC 1 cut(s) 798
PauI GCGCGC 1 cut(s) 358
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 2 cut(s) 86, 1194
PfeI GAWTC 3 cut(s) 226, 733, 1133
PfoI TCCNGGA 2 cut(s) 145, 572
PinAI ACCGGT 1 cut(s) 902
PkrI GCNGC 8 cut(s) 103, 106, 121, 157, 307, 512, 520, 1445
PleI GAGTC 1 cut(s) 445
PmaCI CACGTG 1 cut(s) 239
PmlI CACGTG 1 cut(s) 239
PpsI GAGTC 1 cut(s) 445
Ppu21I YACGTR 2 cut(s) 239, 1261
Psp124BI GAGCTC 1 cut(s) 1098
Psp6I CCWGG 1 cut(s) 572
PspCI CACGTG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 572
PspN4I GGNNCC 7 cut(s) 1043, 1203, 1220, 1273, 1311, 1329, 1419
PspOMI GGGCCC 2 cut(s) 1201, 1218
PspPI GGNCC 9 cut(s) 233, 811, 905, 1201, 1202, 1218, 1219, 1272, 1296
PstNI CAGNNNCTG 2 cut(s) 1019, 1241
PsuI RGATCY 3 cut(s) 1041, 1327, 1525
PteI GCGCGC 1 cut(s) 358
RsaI GTAC 3 cut(s) 502, 642, 1471
RsaNI GTAC 3 cut(s) 501, 641, 1470
RseI CAYNNNNRTG 2 cut(s) 1280, 1598
SacI GAGCTC 1 cut(s) 1098
SalI GTCGAC 1 cut(s) 926
SaqAI TTAA 2 cut(s) 62, 1518
SatI GCNGC 8 cut(s) 102, 105, 120, 156, 306, 511, 519, 1444
Sau3AI GATC 6 cut(s) 583, 757, 784, 1041, 1327, 1525
Sau96I GGNCC 9 cut(s) 233, 811, 905, 1201, 1202, 1218, 1219, 1272, 1296
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 7 cut(s) 147, 231, 232, 574, 1200, 1448, 1449
SduI GDGCHC 6 cut(s) 677, 898, 1098, 1185, 1205, 1222
SfaNI GCATC 8 cut(s) 146, 522, 824, 831, 1050, 1072, 1201, 1522
SfcI CTRYAG 2 cut(s) 1020, 1242
SinI GGWCC 3 cut(s) 811, 905, 1272
SmaI CCCGGG 2 cut(s) 232, 1449
SmiMI CAYNNNNRTG 2 cut(s) 1280, 1598
SmlI CTYRAG 2 cut(s) 852, 1408
SmoI CTYRAG 2 cut(s) 852, 1408
Sse9I AATT 3 cut(s) 12, 769, 876
SspMI CTAG 1 cut(s) 281
SstI GAGCTC 1 cut(s) 1098
StyD4I CCNGG 7 cut(s) 145, 229, 230, 572, 1198, 1446, 1447
StyI CCWWGG 2 cut(s) 794, 1536
TaaI ACNGT 6 cut(s) 425, 449, 1021, 1243, 1422, 1474
TaiI ACGT 6 cut(s) 241, 387, 642, 691, 933, 1263
TaqI TCGA 5 cut(s) 172, 605, 736, 927, 1098
TasI AATT 3 cut(s) 12, 769, 876
TatI WGTACW 1 cut(s) 1469
TauI GCSGC 4 cut(s) 104, 122, 308, 513
TfiI GAWTC 3 cut(s) 226, 733, 1133
Tru1I TTAA 2 cut(s) 62, 1518
Tru9I TTAA 2 cut(s) 62, 1518
TscAI CASTG 1 cut(s) 1338
TseFI GTSAC 2 cut(s) 367, 965
TseI GCWGC 4 cut(s) 104, 155, 518, 1443
Tsp45I GTSAC 2 cut(s) 367, 965
TspDTI ATGAA 6 cut(s) 219, 278, 729, 762, 1076, 1557
TspGWI ACGGA 2 cut(s) 400, 557
TspMI CCCGGG 2 cut(s) 230, 1447
TspRI CASTG 1 cut(s) 1338
VpaK11BI GGWCC 3 cut(s) 811, 905, 1272
XapI RAATTY 1 cut(s) 769
XceI RCATGY 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 1212
XmaI CCCGGG 2 cut(s) 230, 1447
XmiI GTMKAC 2 cut(s) 927, 1542
XspI CTAG 1 cut(s) 281
Zsp2I ATGCAT 2 cut(s) 1065, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.