Rw1G025930

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004640
Physical Location & Seq
Reverse (-)
52792825 .. 52794846
2022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025930.1

Sequence Viewer

Length: 1599 bp
ATGAAAACAGACATTGAAAGCCTCTGGGTTTTCGCCCTTGCTTCGAAATGCAGAGCTCTCTCCCAAGAAAGCATTGCATGTTCTCTTCTTGTCATCGCTCTCTCGTGGCTCGTCATGACTCTCTTTTACTGGGCTCATCCCGGCGGTCCGGCCTGGGGAAGAACCATAAAAAATATTATTACTAGACCAACTGCTATGATTCCCGGTCCGAGAGGTCTGCCGTTGACTGGCAGCATGAGCCTGATGACGGGTCTGGCCCACCGGAAAATCGCCGCCATGGCCGAGGCTTGCAACGCCAAGCGCCTCATGGCGTTCAGCCTGGGCCAGACTCGCGTCATCGTGACGTGTAACCCTGACGTGGCGAGAGAGATTCTCAACAGCTCCGTCTTCGCCGACAGGCCGGTGAAGGAGTCTGCCTACAGTCTGATGTTCAACAGGGCCATCGGGTTCGCCCCATACGGAATGTACTGGCGAACCTTGAGAAGAATCTCGGCTGCGCATCTCTTCTGCCCGAAACAAATCAAGGCCTCTGAGTCGCAACGGTCCGAGATTGCCTCCCAAATGGTGGCAATGTTCGGCGCGCAAGCACAAAACCTGTGGGTGCGCGAGGCCCTCAAGAGAGCCTCCCTCAACAACATGATGTGCTCTGTTTTTGGACGCAAATATGAGCTTGATTCGGTCAGTGACGAAGTCGAAGAGCTCAAGTCGCTAGTGGACGAAGGCTACGACTTATTGGGTAAGTTGAACTGGTCTGACCATCTTCCTTGGCTCGCGGACTTTGACACCCAAAAAATCCGGTTCAGATGCTCCGAACTTGTCCCCAAAGTAAACCGGTTCGTTACCCGGATCATTTCAGAGCACCGGGCCCGAACCGGTGACAAATCCGCCCCGGACTTCGTTGACGTCTTGCTCTCGCTCCAAGGACCCGATAAATTATCCGACTCTGATATGATTGCCGTGCTTTGGGAAATGATATTTAGAGGGACGGACACAGTGGCGGTTCTGATCGAGTGGATTCTGGCGAGGTTGGTGCTTCATCCAGAAGTTCAATCAACGGTGCATGCTGAACTAGACAAAGTAGTTGGGCGGTCCCGAGCCGTTGATGAGTCTGACCTGATTGAGATGGTGTATCTGACGGCTGTGGTGAAAGAGGTGTTGAGGTTGCACCCTCCGGGCCCACTCTTATCATGGGCTCGCTTGGCCATCACCGATACAACAATCGACGGGCGCCACGTGCCTCGGGGGACCACTGCGATGGTCAATATGTGGTCCATTTCGAGGGACCCAGATGTTTGGGCGGATCCGCTAGAATTTAAGCCGGAGAGATTTGTGGCGCAAAAGGATGAGATGGAGTTTTCGGTTTTCGGGTCGGATCTGAGGCTTGCGCCCTTCGGGTCGGGCAGGCGAACTTGCCCGGGAAAGGCGCTGGGGTTGACAACTGTCATGTATTGGGTGGCTTCGCTTTTGCACGAGTACGAATGGATGCCGTCCGATCAGAAGACCGTGGACTTGTCTGAAGTGCTAAAGCTGTCTTGCGAGATGGCCAAGCCTCTGACTGTAAACGTGTGCCCAAGGCGTAGGATGAGCACAAGTCAGTGA

Protein Analysis

532

Amino Acids

59.76

Weight (kDa)

8.44

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 68 - 503 9.2e-81 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 906
Acc16I TGCGCA 1 cut(s) 498
AccB1I GGYRCC 1 cut(s) 1227
AccB7I CCANNNNNTGG 1 cut(s) 565
AccII CGCG 4 cut(s) 333, 581, 606, 773
AciI CCGC 8 cut(s) 144, 273, 773, 885, 998, 1087, 1298, 1304
AclWI GGATC 4 cut(s) 854, 1295, 1308, 1380
AcoI YGGCCR 3 cut(s) 279, 1200, 1542
AcsI RAATTY 1 cut(s) 1310
AcuI CTGAAG 1 cut(s) 1536
AcvI CACGTG 1 cut(s) 1234
AcyI GRCGYC 2 cut(s) 903, 1228
AfaI GTAC 2 cut(s) 467, 1475
AfiI CCNNNNNNNGG 8 cut(s) 155, 227, 247, 358, 565, 963, 1278, 1420
AflIII ACRYGT 2 cut(s) 344, 1563
AgeI ACCGGT 2 cut(s) 831, 872
AgsI TTSAA 4 cut(s) 17, 433, 745, 1049
AjiI CACGTC 2 cut(s) 345, 358
AjnI CCWGG 2 cut(s) 152, 318
AluBI AGCT 5 cut(s) 56, 381, 670, 700, 1528
AluI AGCT 5 cut(s) 56, 381, 670, 700, 1528
Alw21I GWGCWC 5 cut(s) 58, 647, 702, 861, 1589
AlwI GGATC 4 cut(s) 854, 1295, 1308, 1380
Ama87I CYCGRG 3 cut(s) 1092, 1239, 1414
ApaI GGGCCC 2 cut(s) 868, 1178
ApeKI GCWGC 2 cut(s) 231, 494
ApoI RAATTY 1 cut(s) 1310
AsiGI ACCGGT 2 cut(s) 831, 872
AspLEI GCGC 9 cut(s) 303, 499, 581, 583, 606, 1230, 1336, 1387, 1426
AsuC2I CCSGG 8 cut(s) 141, 204, 844, 863, 890, 1173, 1415, 1416
AsuHPI GGTGA 4 cut(s) 415, 887, 1156, 1198
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 3 cut(s) 1092, 1239, 1414
AvaII GGWCC 8 cut(s) 146, 206, 543, 923, 1089, 1245, 1269, 1282
BaeGI GKGCMC 3 cut(s) 868, 1178, 1571
BalI TGGCCA 2 cut(s) 1202, 1544
BamHI GGATCC 1 cut(s) 1300
BanI GGYRCC 1 cut(s) 1227
BanII GRGCYC 6 cut(s) 58, 136, 702, 868, 1178, 1195
BauI CACGAG 2 cut(s) 103, 1469
BbrPI CACGTG 1 cut(s) 1234
BbsI GAAGAC 2 cut(s) 379, 1505
Bbv12I GWGCWC 5 cut(s) 58, 647, 702, 861, 1589
BbvI GCAGC 2 cut(s) 243, 481
BccI CCATC 7 cut(s) 449, 765, 1117, 1211, 1249, 1342, 1534
BceAI ACGGC 5 cut(s) 205, 941, 1082, 1152, 1471
BcgI CGANNNNNNTGC 4 cut(s) 199, 233, 1012, 1046
BciT130I CCWGG 2 cut(s) 154, 320
BcnI CCSGG 8 cut(s) 141, 204, 844, 863, 890, 1173, 1415, 1416
BfaI CTAG 4 cut(s) 183, 710, 1070, 1307
BfmI CTRYAG 1 cut(s) 418
BfoI RGCGCY 3 cut(s) 304, 1231, 1427
BglI GCCNNNNNGGC 1 cut(s) 278
BisI GCNGC 3 cut(s) 232, 273, 495
BlsI GCNGC 3 cut(s) 233, 274, 496
Bme18I GGWCC 8 cut(s) 146, 206, 543, 923, 1089, 1245, 1269, 1282
BmeT110I CYCGRG 3 cut(s) 1092, 1239, 1414
BmgBI CACGTC 2 cut(s) 345, 358
BmiI GGNNCC 9 cut(s) 866, 925, 1091, 1176, 1229, 1246, 1283, 1284, 1302
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 3 cut(s) 508, 794, 1473
BmuI ACTGGG 1 cut(s) 139
BoxI GACNNNNGTC 2 cut(s) 332, 1439
BpiI GAAGAC 2 cut(s) 379, 1505
BplI GAGNNNNNCTC 6 cut(s) 357, 389, 539, 571, 612, 644
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 3 cut(s) 499, 599, 686
BpuMI CCSGG 8 cut(s) 141, 204, 844, 863, 890, 1173, 1415, 1416
BsaAI YACGTR 1 cut(s) 1234
BsaHI GRCGYC 2 cut(s) 903, 1228
BsaWI WCCGGW 4 cut(s) 261, 795, 831, 872
Bsc4I CCNNNNNNNGG 8 cut(s) 155, 227, 247, 358, 565, 963, 1278, 1420
Bse118I RCCGGY 3 cut(s) 400, 831, 872
Bse1I ACTGG 4 cut(s) 134, 232, 473, 752
Bse3DI GCAATG 2 cut(s) 72, 576
BseBI CCWGG 2 cut(s) 154, 320
BseGI GGATG 5 cut(s) 136, 1036, 1348, 1488, 1587
BseLI CCNNNNNNNGG 8 cut(s) 155, 227, 247, 358, 565, 963, 1278, 1420
BseMI GCAATG 2 cut(s) 72, 576
BseMII CTCAG 2 cut(s) 522, 1367
BseNI ACTGG 4 cut(s) 134, 232, 473, 752
BsePI GCGCGC 1 cut(s) 579
BseSI GKGCMC 3 cut(s) 868, 1178, 1571
BseXI GCAGC 2 cut(s) 243, 481
BseYI CCCAGC 1 cut(s) 1426
Bsh1236I CGCG 4 cut(s) 333, 581, 606, 773
BshNI GGYRCC 1 cut(s) 1227
BshTI ACCGGT 2 cut(s) 831, 872
BsiHKAI GWGCWC 5 cut(s) 58, 647, 702, 861, 1589
BsiHKCI CYCGRG 3 cut(s) 1092, 1239, 1414
BslFI GGGAC 5 cut(s) 803, 997, 1075, 1258, 1295
BslI CCNNNNNNNGG 8 cut(s) 155, 227, 247, 358, 565, 963, 1278, 1420
BsmFI GGGAC 5 cut(s) 803, 997, 1075, 1258, 1295
BsoBI CYCGRG 3 cut(s) 1092, 1239, 1414
Bsp119I TTCGAA 1 cut(s) 44
Bsp120I GGGCCC 2 cut(s) 864, 1174
Bsp143I GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 8 cut(s) 144, 273, 773, 885, 998, 1087, 1298, 1304
BspCNI CTCAG 2 cut(s) 523, 1368
BspFNI CGCG 4 cut(s) 333, 581, 606, 773
BspHI TCATGA 1 cut(s) 114
BspLI GGNNCC 9 cut(s) 866, 925, 1091, 1176, 1229, 1246, 1283, 1284, 1302
BspPI GGATC 4 cut(s) 854, 1295, 1308, 1380
BspQI GCTCTTC 1 cut(s) 690
BspT104I TTCGAA 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 1227
BsrDI GCAATG 2 cut(s) 72, 576
BsrFI RCCGGY 3 cut(s) 400, 831, 872
BsrI ACTGG 4 cut(s) 134, 232, 473, 752
BssAI RCCGGY 3 cut(s) 400, 831, 872
BssHII GCGCGC 1 cut(s) 579
BssMI GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
BssNI GRCGYC 2 cut(s) 903, 1228
BssSI CACGAG 2 cut(s) 103, 1469
BssT1I CCWWGG 4 cut(s) 276, 764, 919, 1571
Bst2BI CACGAG 2 cut(s) 103, 1469
Bst2UI CCWGG 2 cut(s) 154, 320
Bst4CI ACNGT 7 cut(s) 422, 543, 994, 1057, 1441, 1504, 1558
Bst6I CTCTTC 3 cut(s) 90, 509, 690
BstACI GRCGYC 2 cut(s) 903, 1228
BstBAI YACGTR 1 cut(s) 1234
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 8 cut(s) 289, 581, 585, 771, 1062, 1195, 1383, 1403
BstDEI CTNAG 2 cut(s) 531, 1376
BstDSI CCRYGG 2 cut(s) 276, 1503
BstF5I GGATG 5 cut(s) 136, 1036, 1348, 1488, 1587
BstFNI CGCG 4 cut(s) 333, 581, 606, 773
BstH2I RGCGCY 3 cut(s) 304, 1231, 1427
BstHHI GCGC 9 cut(s) 303, 499, 581, 583, 606, 1230, 1336, 1387, 1426
BstKTI GATC 5 cut(s) 849, 1008, 1303, 1375, 1495
BstMBI GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
BstMWI GCNNNNNNNGC 7 cut(s) 237, 278, 293, 330, 706, 1199, 1234
BstNI CCWGG 2 cut(s) 154, 320
BstNSI RCATGY 2 cut(s) 81, 1064
BstPAI GACNNNNGTC 2 cut(s) 332, 1439
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 3 cut(s) 868, 1178, 1571
BstUI CGCG 4 cut(s) 333, 581, 606, 773
BstV1I GCAGC 2 cut(s) 243, 481
BstV2I GAAGAC 2 cut(s) 379, 1505
BstX2I RGATCY 2 cut(s) 1300, 1372
BstXI CCANNNNNNTGG 2 cut(s) 1255, 1293
BstYI RGATCY 2 cut(s) 1300, 1372
BtgI CCRYGG 2 cut(s) 276, 1503
BtgZI GCGATG 2 cut(s) 79, 1268
BtrI CACGTC 2 cut(s) 345, 358
BtsCI GGATG 5 cut(s) 136, 1036, 1348, 1488, 1587
BtsI GCAGTG 1 cut(s) 1248
BtsIMutI CAGTG 3 cut(s) 688, 999, 1248
Cac8I GCNNGC 8 cut(s) 289, 581, 585, 771, 1062, 1195, 1383, 1403
CciI TCATGA 1 cut(s) 114
CfoI GCGC 9 cut(s) 303, 499, 581, 583, 606, 1230, 1336, 1387, 1426
Cfr10I RCCGGY 3 cut(s) 400, 831, 872
Cfr9I CCCGGG 1 cut(s) 1414
CpoI CGGWCCG 3 cut(s) 146, 206, 543
CseI GACGC 2 cut(s) 322, 666
Csp6I GTAC 2 cut(s) 466, 1474
CspAI ACCGGT 2 cut(s) 831, 872
CspCI CAANNNNNGTGG 2 cut(s) 578, 613
CspI CGGWCCG 3 cut(s) 146, 206, 543
CviAII CATG 9 cut(s) 78, 115, 235, 277, 307, 637, 1061, 1188, 1444
CviQI GTAC 2 cut(s) 466, 1474
DdeI CTNAG 2 cut(s) 531, 1376
DinI GGCGCC 1 cut(s) 1229
DpnI GATC 5 cut(s) 848, 1007, 1302, 1374, 1494
DpnII GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
EaeI YGGCCR 3 cut(s) 279, 1200, 1542
Eam1104I CTCTTC 3 cut(s) 90, 509, 690
EarI CTCTTC 3 cut(s) 90, 509, 690
EciI GGCGGA 2 cut(s) 874, 1313
Ecl136II GAGCTC 2 cut(s) 56, 700
Eco130I CCWWGG 4 cut(s) 276, 764, 919, 1571
Eco147I AGGCCT 1 cut(s) 527
Eco24I GRGCYC 6 cut(s) 58, 136, 702, 868, 1178, 1195
Eco47I GGWCC 8 cut(s) 146, 206, 543, 923, 1089, 1245, 1269, 1282
Eco53kI GAGCTC 2 cut(s) 56, 700
Eco57I CTGAAG 1 cut(s) 1536
Eco72I CACGTG 1 cut(s) 1234
Eco88I CYCGRG 3 cut(s) 1092, 1239, 1414
EcoICRI GAGCTC 2 cut(s) 56, 700
EcoO109I RGGNCCY 3 cut(s) 610, 923, 1282
EcoRII CCWGG 2 cut(s) 152, 318
EcoT14I CCWWGG 4 cut(s) 276, 764, 919, 1571
EcoT38I GRGCYC 6 cut(s) 58, 136, 702, 868, 1178, 1195
EgeI GGCGCC 1 cut(s) 1229
EheI GGCGCC 1 cut(s) 1229
ErhI CCWWGG 4 cut(s) 276, 764, 919, 1571
FaeI CATG 9 cut(s) 81, 118, 238, 280, 310, 640, 1064, 1191, 1447
FaqI GGGAC 5 cut(s) 803, 997, 1075, 1258, 1295
FatI CATG 9 cut(s) 77, 114, 234, 276, 306, 636, 1060, 1187, 1443
Fnu4HI GCNGC 3 cut(s) 232, 273, 495
FokI GGATG 5 cut(s) 123, 1023, 1355, 1495, 1594
FriOI GRGCYC 6 cut(s) 58, 136, 702, 868, 1178, 1195
Fsp4HI GCNGC 3 cut(s) 232, 273, 495
FspBI CTAG 4 cut(s) 183, 710, 1070, 1307
FspI TGCGCA 1 cut(s) 498
GlaI GCGC 9 cut(s) 302, 498, 580, 582, 605, 1229, 1335, 1386, 1425
GluI GCNGC 3 cut(s) 232, 273, 495
GsaI CCCAGC 1 cut(s) 1430
HaeII RGCGCY 3 cut(s) 304, 1231, 1427
HgaI GACGC 2 cut(s) 322, 666
HhaI GCGC 9 cut(s) 303, 499, 581, 583, 606, 1230, 1336, 1387, 1426
Hin1I GRCGYC 2 cut(s) 903, 1228
Hin1II CATG 9 cut(s) 81, 118, 238, 280, 310, 640, 1064, 1191, 1447
Hin6I GCGC 9 cut(s) 301, 497, 579, 581, 604, 1228, 1334, 1385, 1424
HinP1I GCGC 9 cut(s) 301, 497, 579, 581, 604, 1228, 1334, 1385, 1424
HincII GTYRAC 3 cut(s) 225, 901, 1434
HindII GTYRAC 3 cut(s) 225, 901, 1434
HphI GGTGA 4 cut(s) 415, 887, 1156, 1198
Hpy166II GTNNAC 7 cut(s) 225, 715, 829, 901, 1434, 1507, 1561
Hpy188III TCNNGA 5 cut(s) 115, 340, 616, 1040, 1092
Hpy8I GTNNAC 7 cut(s) 225, 715, 829, 901, 1434, 1507, 1561
Hpy99I CGWCG 1 cut(s) 1226
HpyAV CCTTC 3 cut(s) 400, 713, 1399
HpyCH4III ACNGT 7 cut(s) 422, 543, 994, 1057, 1441, 1504, 1558
HpyCH4IV ACGT 5 cut(s) 344, 357, 903, 1233, 1563
HpyCH4V TGCA 6 cut(s) 51, 77, 291, 1060, 1165, 1468
HpyF10VI GCNNNNNNNGC 7 cut(s) 237, 278, 293, 330, 706, 1199, 1234
HpyF3I CTNAG 2 cut(s) 531, 1376
HpySE526I ACGT 5 cut(s) 344, 357, 903, 1233, 1563
Hsp92I GRCGYC 2 cut(s) 903, 1228
Hsp92II CATG 9 cut(s) 81, 118, 238, 280, 310, 640, 1064, 1191, 1447
HspAI GCGC 9 cut(s) 301, 497, 579, 581, 604, 1228, 1334, 1385, 1424
KasI GGCGCC 1 cut(s) 1227
KflI GGGWCCC 1 cut(s) 1282
Kzo9I GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 3 cut(s) 386, 812, 921
Lsp1109I GCAGC 2 cut(s) 243, 481
LweI GCATC 3 cut(s) 508, 794, 1473
MaeI CTAG 4 cut(s) 183, 710, 1070, 1307
MaeII ACGT 5 cut(s) 344, 357, 903, 1233, 1563
MaeIII GTNAC 5 cut(s) 340, 347, 683, 838, 875
MalI GATC 5 cut(s) 848, 1007, 1302, 1374, 1494
MboI GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
MboII GAAGA 8 cut(s) 77, 171, 379, 495, 496, 707, 752, 1510
MflI RGATCY 2 cut(s) 1300, 1372
MlsI TGGCCA 2 cut(s) 1202, 1544
MluCI AATT 2 cut(s) 932, 1310
MluNI TGGCCA 2 cut(s) 1202, 1544
Mly113I GGCGCC 1 cut(s) 1228
MlyI GAGTC 6 cut(s) 112, 322, 419, 542, 935, 1115
MmeI TCCRAC 2 cut(s) 963, 1350
Mox20I TGGCCA 2 cut(s) 1202, 1544
MscI TGGCCA 2 cut(s) 1202, 1544
MseI TTAA 1 cut(s) 1314
MslI CAYNNNNRTG 1 cut(s) 1253
Msp20I TGGCCA 2 cut(s) 1202, 1544
MvaI CCWGG 2 cut(s) 154, 320
MvnI CGCG 4 cut(s) 333, 581, 606, 773
MwoI GCNNNNNNNGC 7 cut(s) 237, 278, 293, 330, 706, 1199, 1234
NarI GGCGCC 1 cut(s) 1228
NciI CCSGG 8 cut(s) 141, 204, 844, 863, 890, 1173, 1415, 1416
NcoI CCATGG 1 cut(s) 276
NdeII GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
NlaIII CATG 9 cut(s) 81, 118, 238, 280, 310, 640, 1064, 1191, 1447
NlaIV GGNNCC 9 cut(s) 866, 925, 1091, 1176, 1229, 1246, 1283, 1284, 1302
NmeAIII GCCGAG 2 cut(s) 307, 470
NmuCI GTSAC 3 cut(s) 340, 683, 875
NsbI TGCGCA 1 cut(s) 498
NspI RCATGY 2 cut(s) 81, 1064
NspV TTCGAA 1 cut(s) 44
PaeI GCATGC 1 cut(s) 1064
PagI TCATGA 1 cut(s) 114
PauI GCGCGC 1 cut(s) 579
PceI AGGCCT 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 690
PcsI WCGNNNNNNNCGW 2 cut(s) 723, 1230
PfeI GAWTC 5 cut(s) 199, 370, 486, 674, 1015
PflFI GACNNNGTC 1 cut(s) 689
PflMI CCANNNNNTGG 1 cut(s) 565
PinAI ACCGGT 2 cut(s) 831, 872
PkrI GCNGC 3 cut(s) 233, 274, 496
PleI GAGTC 6 cut(s) 112, 322, 418, 541, 935, 1114
PluTI GGCGCC 1 cut(s) 1231
PmaCI CACGTG 1 cut(s) 1234
PmlI CACGTG 1 cut(s) 1234
PpsI GAGTC 6 cut(s) 112, 322, 418, 541, 935, 1114
Ppu21I YACGTR 1 cut(s) 1234
PpuMI RGGWCCY 2 cut(s) 923, 1282
PshAI GACNNNNGTC 2 cut(s) 332, 1439
Psp124BI GAGCTC 2 cut(s) 58, 702
Psp5II RGGWCCY 2 cut(s) 923, 1282
Psp6I CCWGG 2 cut(s) 152, 318
PspCI CACGTG 1 cut(s) 1234
PspFI CCCAGC 1 cut(s) 1426
PspGI CCWGG 2 cut(s) 152, 318
PspN4I GGNNCC 9 cut(s) 866, 925, 1091, 1176, 1229, 1246, 1283, 1284, 1302
PspOMI GGGCCC 2 cut(s) 864, 1174
PspPPI RGGWCCY 2 cut(s) 923, 1282
PsuI RGATCY 2 cut(s) 1300, 1372
PsyI GACNNNGTC 1 cut(s) 689
PteI GCGCGC 1 cut(s) 579
RsaI GTAC 2 cut(s) 467, 1475
RsaNI GTAC 2 cut(s) 466, 1474
RseI CAYNNNNRTG 1 cut(s) 1253
Rsr2I CGGWCCG 3 cut(s) 146, 206, 543
RsrII CGGWCCG 3 cut(s) 146, 206, 543
SacI GAGCTC 2 cut(s) 58, 702
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 1 cut(s) 1314
SatI GCNGC 3 cut(s) 232, 273, 495
Sau3AI GATC 5 cut(s) 846, 1005, 1300, 1372, 1492
SchI GAGTC 6 cut(s) 112, 322, 419, 542, 935, 1115
SfaNI GCATC 3 cut(s) 508, 794, 1473
SfcI CTRYAG 1 cut(s) 418
SfoI GGCGCC 1 cut(s) 1229
SfuI TTCGAA 1 cut(s) 44
SinI GGWCC 8 cut(s) 146, 206, 543, 923, 1089, 1245, 1269, 1282
SmaI CCCGGG 1 cut(s) 1416
SmiMI CAYNNNNRTG 1 cut(s) 1253
SmlI CTYRAG 3 cut(s) 478, 614, 701
SmoI CTYRAG 3 cut(s) 478, 614, 701
SphI GCATGC 1 cut(s) 1064
Sse9I AATT 2 cut(s) 932, 1310
SseBI AGGCCT 1 cut(s) 527
SsiI CCGC 8 cut(s) 144, 273, 773, 885, 998, 1087, 1298, 1304
SspDI GGCGCC 1 cut(s) 1227
SspI AATATT 1 cut(s) 175
SspMI CTAG 4 cut(s) 183, 710, 1070, 1307
SstI GAGCTC 2 cut(s) 58, 702
StuI AGGCCT 1 cut(s) 527
StyI CCWWGG 4 cut(s) 276, 764, 919, 1571
TaaI ACNGT 7 cut(s) 422, 543, 994, 1057, 1441, 1504, 1558
TaiI ACGT 5 cut(s) 347, 360, 906, 1236, 1566
TaqI TCGA 5 cut(s) 44, 693, 1008, 1221, 1277
TaqII GACCGA 1 cut(s) 667
TasI AATT 2 cut(s) 932, 1310
TatI WGTACW 1 cut(s) 465
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 5 cut(s) 199, 370, 486, 674, 1015
Tru1I TTAA 1 cut(s) 1314
Tru9I TTAA 1 cut(s) 1314
TscAI CASTG 3 cut(s) 688, 999, 1255
TseFI GTSAC 3 cut(s) 340, 683, 875
TseI GCWGC 2 cut(s) 231, 494
Tsp45I GTSAC 3 cut(s) 340, 683, 875
TspDTI ATGAA 2 cut(s) 17, 1025
TspGWI ACGGA 3 cut(s) 373, 474, 1001
TspMI CCCGGG 1 cut(s) 1414
TspRI CASTG 3 cut(s) 688, 999, 1255
Tth111I GACNNNGTC 1 cut(s) 689
Van91I CCANNNNNTGG 1 cut(s) 565
VpaK11BI GGWCC 8 cut(s) 146, 206, 543, 923, 1089, 1245, 1269, 1282
XapI RAATTY 1 cut(s) 1310
XceI RCATGY 2 cut(s) 81, 1064
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 1185
XmaI CCCGGG 1 cut(s) 1414
XspI CTAG 4 cut(s) 183, 710, 1070, 1307
ZraI GACGTC 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.