Rorug06G0478400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004640
Physical Location & Seq
Reverse (-)
60028082 .. 60031472
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0478400.1

Sequence Viewer

Length: 762 bp
ATGGGCCTTAAGATTTTCAGAACAAATCAAAATCAAACCGCGAGAACCCCGACTCACGCGCTAGTCAGTAGTCACTACCATGATCCACGATACTCCCTCCTCCGCCAAACAAAGACTGAGCGACTGATTGCCGATATGGGATCCTTGAATCCTATGCTTCTCAGAGGCGTATCTCTCCTCCGCGTCCGTCTCCACCACCACCACCACCGGCTGGGCTTTGGGTTTGGGATTGGGATTGCGGGGGCGCCGAGTCGCAGGGCGTGCTGCACCGAGTCGATTCGGGCGGAGGAGAGTAGTTCGTCCTCTGCGGACAACTCCTCCTCGACGCTAAGAGGCGTGCTGAGCGTGAACGAGGCACCCAAGTATCCGCGGTGGGACGACCCTGACTACTGCAAGTGGAAAGATAAGGAAGGCGAGATTCTGAGGGACGTGGAGCCTATAGTTTTGCTTGCCAAAGAGATTCTCCATTCGGATAGGTATATGGATGGTGAGCAGCTAACACTCGAAGATGAAAAAGTTGTGGTGGAAAGGCTTCTGGCGTATCATCCGCATTCCGAAGATAAAATTGGCTGCGGAATTGATTCTATAATGGTTGATCGGCATCCCCAATTCAAACACTCAAGGTGCCTATTTGTCATAAGAACTGATAGTGTATGGATAGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATAAGTACCCAACCCATGCAGAAAGATTCATTCGAGAACATTTTAAACGTGGTAGTGGCTAA

Protein Analysis

253

Amino Acids

29.15

Weight (kDa)

8.86

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 152 - 228 4.6e-30 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 244, 355, 624
AccB7I CCANNNNNTGG 1 cut(s) 211
AccII CGCG 4 cut(s) 41, 59, 183, 370
AclWI GGATC 3 cut(s) 77, 135, 148
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 1 cut(s) 707
AfiI CCNNNNNNNGG 1 cut(s) 211
AflII CTTAAG 1 cut(s) 8
AgsI TTSAA 2 cut(s) 148, 613
AjiI CACGTC 1 cut(s) 430
AjuI GAANNNNNNNTTGG 2 cut(s) 549, 581
AluBI AGCT 1 cut(s) 496
AluI AGCT 1 cut(s) 496
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 3 cut(s) 77, 135, 148
AoxI GGCC 1 cut(s) 4
ApeKI GCWGC 3 cut(s) 264, 493, 570
Asp700I GAANNNNTTC 2 cut(s) 531, 580
AspLEI GCGC 2 cut(s) 61, 247
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 500
BaeI ACNNNNGTAYC 2 cut(s) 347, 380
BamHI GGATCC 1 cut(s) 140
BanI GGYRCC 3 cut(s) 244, 355, 624
BbsI GAAGAC 1 cut(s) 674
BbvI GCAGC 3 cut(s) 251, 505, 557
BccI CCATC 1 cut(s) 479
BciVI GTATCC 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 248
BfrI CTTAAG 1 cut(s) 8
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 3 cut(s) 265, 494, 571
BlpI GCTNAGC 1 cut(s) 341
BlsI GCNGC 3 cut(s) 266, 495, 572
BmgBI CACGTC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 5 cut(s) 142, 246, 357, 435, 626
BmsI GCATC 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 674
Bpu1102I GCTNAGC 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 604
BsaBI GATNNNNATC 1 cut(s) 600
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 368
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse118I RCCGGY 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 368
BseGI GGATG 3 cut(s) 490, 544, 601
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 4 cut(s) 108, 175, 332, 413
BseRI GAGGAG 5 cut(s) 89, 167, 302, 307, 310
BseXI GCAGC 3 cut(s) 251, 505, 557
BseYI CCCAGC 1 cut(s) 211
BsgI GTGCAG 1 cut(s) 250
Bsh1236I CGCG 4 cut(s) 41, 59, 183, 370
BshFI GGCC 1 cut(s) 6
BshNI GGYRCC 3 cut(s) 244, 355, 624
BsiSI CCGG 1 cut(s) 208
BslFI GGGAC 2 cut(s) 389, 440
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 194
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 2 cut(s) 389, 440
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 1 cut(s) 6
Bsp143I GATC 3 cut(s) 82, 140, 595
Bsp1720I GCTNAGC 1 cut(s) 341
BspANI GGCC 1 cut(s) 6
BspCNI CTCAG 4 cut(s) 109, 174, 333, 414
BspFNI CGCG 4 cut(s) 41, 59, 183, 370
BspLI GGNNCC 5 cut(s) 142, 246, 357, 435, 626
BspPI GGATC 3 cut(s) 77, 135, 148
BspT107I GGYRCC 3 cut(s) 244, 355, 624
BspTI CTTAAG 1 cut(s) 8
BsrFI RCCGGY 1 cut(s) 207
BssAI RCCGGY 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 3 cut(s) 82, 140, 595
BssNI GRCGYC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 245
BstAFI CTTAAG 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 262, 338, 450
BstDEI CTNAG 5 cut(s) 117, 161, 329, 341, 422
BstDSI CCRYGG 1 cut(s) 368
BstF5I GGATG 3 cut(s) 490, 544, 601
BstFNI CGCG 4 cut(s) 41, 59, 183, 370
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 2 cut(s) 61, 247
BstKTI GATC 3 cut(s) 85, 143, 598
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 3 cut(s) 82, 140, 595
BstMWI GCNNNNNNNGC 2 cut(s) 261, 342
BstSFI CTRYAG 1 cut(s) 438
BstUI CGCG 4 cut(s) 41, 59, 183, 370
BstV1I GCAGC 3 cut(s) 251, 505, 557
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 1 cut(s) 6
BtgI CCRYGG 1 cut(s) 368
BtrI CACGTC 1 cut(s) 430
BtsCI GGATG 3 cut(s) 490, 544, 601
Cac8I GCNNGC 3 cut(s) 262, 338, 450
CfoI GCGC 2 cut(s) 61, 247
Cfr10I RCCGGY 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 4
Cfr42I CCGCGG 1 cut(s) 371
CseI GACGC 2 cut(s) 172, 334
Csp6I GTAC 1 cut(s) 706
CviAII CATG 2 cut(s) 80, 716
CviJI RGCY 8 cut(s) 6, 211, 216, 436, 496, 532, 570, 759
CviKI_1 RGCY 8 cut(s) 6, 211, 216, 436, 496, 532, 570, 759
CviQI GTAC 1 cut(s) 706
DdeI CTNAG 5 cut(s) 117, 161, 329, 341, 422
DinI GGCGCC 1 cut(s) 246
DpnI GATC 3 cut(s) 84, 142, 597
DpnII GATC 3 cut(s) 82, 140, 595
DraI TTTAAA 1 cut(s) 745
EciI GGCGGA 2 cut(s) 92, 299
EgeI GGCGCC 1 cut(s) 246
EheI GGCGCC 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 2 cut(s) 83, 719
FaqI GGGAC 2 cut(s) 389, 440
FatI CATG 2 cut(s) 79, 715
FauI CCCGC 1 cut(s) 232
Fnu4HI GCNGC 3 cut(s) 265, 494, 571
FokI GGATG 3 cut(s) 497, 531, 588
Fsp4HI GCNGC 3 cut(s) 265, 494, 571
FspBI CTAG 1 cut(s) 62
GlaI GCGC 2 cut(s) 60, 246
GluI GCNGC 3 cut(s) 265, 494, 571
GsaI CCCAGC 1 cut(s) 215
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 1 cut(s) 6
HapII CCGG 1 cut(s) 208
HgaI GACGC 2 cut(s) 172, 334
HhaI GCGC 2 cut(s) 61, 247
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 2 cut(s) 83, 719
Hin6I GCGC 2 cut(s) 59, 245
HinP1I GCGC 2 cut(s) 59, 245
HinfI GANTC 9 cut(s) 52, 148, 250, 272, 277, 418, 460, 581, 726
HpaII CCGG 1 cut(s) 208
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 6 cut(s) 20, 164, 423, 472, 556, 698
Hpy188III TCNNGA 1 cut(s) 734
Hpy8I GTNNAC 1 cut(s) 349
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 404
HpyCH4IV ACGT 2 cut(s) 429, 748
HpyCH4V TGCA 3 cut(s) 267, 393, 719
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 342
HpyF3I CTNAG 5 cut(s) 117, 161, 329, 341, 422
HpySE526I ACGT 2 cut(s) 429, 748
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 2 cut(s) 83, 719
HspAI GCGC 2 cut(s) 59, 245
KasI GGCGCC 1 cut(s) 244
KspI CCGCGG 1 cut(s) 371
Kzo9I GATC 3 cut(s) 82, 140, 595
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 6 cut(s) 197, 221, 241, 396, 521, 686
Lsp1109I GCAGC 3 cut(s) 251, 505, 557
LweI GCATC 1 cut(s) 610
MaeI CTAG 1 cut(s) 62
MaeII ACGT 2 cut(s) 429, 748
MaeIII GTNAC 1 cut(s) 71
MalI GATC 3 cut(s) 84, 142, 597
MboI GATC 3 cut(s) 82, 140, 595
MboII GAAGA 3 cut(s) 518, 569, 674
MflI RGATCY 1 cut(s) 140
MluCI AATT 3 cut(s) 564, 576, 608
Mly113I GGCGCC 1 cut(s) 245
MlyI GAGTC 3 cut(s) 46, 259, 281
MroXI GAANNNNTTC 2 cut(s) 531, 580
MseI TTAA 2 cut(s) 9, 744
MslI CAYNNNNRTG 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 370
MspCI CTTAAG 1 cut(s) 8
MspI CCGG 1 cut(s) 208
Mva1269I GAATGC 1 cut(s) 550
MvnI CGCG 4 cut(s) 41, 59, 183, 370
MwoI GCNNNNNNNGC 2 cut(s) 261, 342
NarI GGCGCC 1 cut(s) 245
NdeII GATC 3 cut(s) 82, 140, 595
NlaIII CATG 2 cut(s) 83, 719
NlaIV GGNNCC 5 cut(s) 142, 246, 357, 435, 626
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 71
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 2 cut(s) 531, 580
PfeI GAWTC 6 cut(s) 148, 277, 418, 460, 581, 726
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 3 cut(s) 266, 495, 572
PleI GAGTC 3 cut(s) 46, 258, 280
PluTI GGCGCC 1 cut(s) 248
PpsI GAGTC 3 cut(s) 46, 258, 280
PspFI CCCAGC 1 cut(s) 211
PspN4I GGNNCC 5 cut(s) 142, 246, 357, 435, 626
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 1 cut(s) 140
RsaI GTAC 1 cut(s) 707
RsaNI GTAC 1 cut(s) 706
RseI CAYNNNNRTG 1 cut(s) 78
SacII CCGCGG 1 cut(s) 371
SaqAI TTAA 2 cut(s) 9, 744
SatI GCNGC 3 cut(s) 265, 494, 571
Sau3AI GATC 3 cut(s) 82, 140, 595
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 3 cut(s) 46, 259, 281
SetI ASST 5 cut(s) 432, 479, 498, 626, 751
SfaNI GCATC 1 cut(s) 610
SfcI CTRYAG 1 cut(s) 438
SfoI GGCGCC 1 cut(s) 246
Sfr303I CCGCGG 1 cut(s) 371
SgrBI CCGCGG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 2 cut(s) 8, 619
SmoI CTYRAG 2 cut(s) 8, 619
Sse9I AATT 3 cut(s) 564, 576, 608
SspDI GGCGCC 1 cut(s) 244
SspMI CTAG 1 cut(s) 62
TaiI ACGT 2 cut(s) 432, 751
TaqI TCGA 5 cut(s) 275, 323, 504, 685, 733
TasI AATT 3 cut(s) 564, 576, 608
TfiI GAWTC 6 cut(s) 148, 277, 418, 460, 581, 726
Tru1I TTAA 2 cut(s) 9, 744
Tru9I TTAA 2 cut(s) 9, 744
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 3 cut(s) 264, 493, 570
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 2 cut(s) 525, 718
TspGWI ACGGA 1 cut(s) 176
Van91I CCANNNNNTGG 1 cut(s) 211
Vha464I CTTAAG 1 cut(s) 8
XmnI GAANNNNTTC 2 cut(s) 531, 580
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.