pycom07g15270

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004640
Physical Location & Seq
Reverse (-)
17468551 .. 17470421
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g15270.1

Sequence Viewer

Length: 1782 bp
ATGCAACATACCTTCCCCTATATATACCCGTCATCAGCCGGTGTTTGGTTCAAACTCAAAAGCTACTCCTCCTTTCCTTCTTTTTTTCTCACTGCAAAAGGAAGCCAACCCTACTCTCTCTCTCTCTCTCTCTCTCTCAGACTACTAGTCGCCATGAAAACAGAGTTAGAGATAGAAAGGCTATGGGTATTTGCCCTGGCCTCCAAATGCAGAGCCTTCTCTCCCGAAAGCATTGCATGCACGCTTCTCATCGTTTCCCTCACATGGCTTCTCATCTCCCTAATTTACTGGTCTCACCCGGGAGGCCCAGCCTGGGGCTGCAGCATCAAAATAATCGGTCCCTTTTCCAAAAGGCCCACAATTCCGGGGCCCAGAGGCCTGCCTCTCATAGGCAGCATGTCCCTAATGACCTCCCTGGCCCATCGTAAAATCGCCTCCACTGCCCAATCCCTCCATGCCATGCGCCTCATGGCCTTCAGCCTCGGCCAAACCAGAGTCGTCGTCACGTGCCACCCCGACGTCGCCAGAGAAATCCTCAACAGCTCCGTCTTCGCCGACCGCCCCGTCAAGGAATCCGCCTACTCCCTCATGTTCAACCGGGCCATCGGGTTCGCCCCCAACGGCGTCTACTGGCGGGCCCTCAGGCGTATCTCCGCCGCCCACCTCTTCTCCCCCAAGCAAATCAAGGCCTCCGAGGCCCAGAGGAGGGACATAGCTTCTCAAATGGCGGCAATGTTCGGGAGCTACGAGGAGAAATTGGCCGTTCGTAACGTGATCAAGCGGGCGTCGCTGAACAACATGATGTGCTCTGTTTTTGGCAGGAAGTACGAGCTCGAAGTCGGCGCCGCCGTGGACAGTGAAGTCGAAGAGCTCAAAGGGTTGGTAGACGAAGGCTACGATTTGTTGGGTATGTTAAATTGGTCCGATCATCTTCCCTGGTTGGCCGATTTTGATGCCCAGAAAATCCGGTTCAGATGCTCCAATCTCGTCCCCAAAGTGAAACGTTTCGTCTCAAGAATTATTTCCGAGCACCGAACCGGTCTGAAATCCAAAAAAGATGAAAAGGCCCCCGACTTTGTCGACGTTCTTCTTTCCCTCCAAGGAACCGATAAGTTGTCCGACACCGATATGATCGCCGTCCTTTGGGAAATGATATTTAGAGGGACGGACACCGTGGCGGTTCTGATCGAGTGGATTCTGGCAAGGATGGTGCTGCACCCTATTGTTCAATCGACGATCCACGACGAGCTAGATAAAGTGGTTGGAAGGTCGCGAGCCGTCGAGGAGACAGATATCTCCCAGCTGGTGTATCTAACGGCTGTGGTGAAGGAGGTCTTGAGGATGCACCCACCGGGCCCACTTTTATCTTGGGCCCGTCTGGCGATCACTGATACAATAATTGATGGATATCATGTGCCCGCAGGGACCACAGCCATGGTGAATATGTGGGCAATCTCTCGGGATCCGGAGTTTTGGCCGGACCCACTAGAGTTTAAGCCCGAGAGGTTTGTGGCTCAGGAAGGAGAACATGAGTTTTCGGTCTTCGGGTCGGATCTTAGGCTTGCACCGTTTGGATCGGGTAGGCGGACTTGCCCAGGAAAAGCTCTTGGATTGACCACTGTGACCTTTTGGGTGGCGTCGCTTTTACACGAGTATGAGTGGCAACCGTCGGATGGGAATATCGTGGACTTATCGGAGGTTCTAAGGCTGTCATGTGAGATGGCTAGCCCTCTGGTTGCTAAAGTGCGCCCAAGGTGTAGTAGGGTAACCACAGCTCACTAA

Protein Analysis

594

Amino Acids

66.35

Weight (kDa)

8.9

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 122 - 560 3e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 563, 860
AatII GACGTC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 842
AccI GTMKAC 3 cut(s) 627, 885, 1080
AccII CGCG 1 cut(s) 1273
AccIII TCCGGA 1 cut(s) 1465
AclI AACGTT 1 cut(s) 1003
AclWI GGATC 5 cut(s) 1231, 1457, 1470, 1560, 1582
AcoI YGGCCR 4 cut(s) 484, 759, 942, 1475
AcuI CTGAAG 1 cut(s) 460
AcvI CACGTG 1 cut(s) 507
AcyI GRCGYC 5 cut(s) 519, 624, 785, 843, 1637
AfaI GTAC 1 cut(s) 827
AgeI ACCGGT 1 cut(s) 1037
AgsI TTSAA 3 cut(s) 52, 595, 1229
AhdI GACNNNNNGTC 1 cut(s) 146
AhlI ACTAGT 1 cut(s) 145
AjnI CCWGG 5 cut(s) 195, 311, 414, 935, 1594
Alw21I GWGCWC 4 cut(s) 809, 834, 873, 1032
Alw26I GTCTC 3 cut(s) 297, 1015, 1280
AlwI GGATC 5 cut(s) 1231, 1457, 1470, 1560, 1582
Ama87I CYCGRG 3 cut(s) 298, 1458, 1499
Aor13HI TCCGGA 1 cut(s) 1465
ApaI GGGCCC 4 cut(s) 372, 640, 1358, 1375
ApeKI GCWGC 4 cut(s) 318, 321, 393, 1213
AsiGI ACCGGT 1 cut(s) 1037
Asp700I GAANNNNTTC 2 cut(s) 1004, 1021
AspLEI GCGC 3 cut(s) 465, 845, 1749
AsuC2I CCSGG 5 cut(s) 299, 300, 366, 599, 1353
AsuHPI GGTGA 3 cut(s) 287, 1336, 1450
AsuNHI GCTAGC 1 cut(s) 1724
AvaI CYCGRG 3 cut(s) 298, 1458, 1499
AvaII GGWCC 4 cut(s) 338, 921, 1425, 1480
AxyI CCTNAGG 1 cut(s) 641
BaeGI GKGCMC 5 cut(s) 372, 640, 1358, 1375, 1419
BamHI GGATCC 1 cut(s) 1462
BanI GGYRCC 1 cut(s) 842
BanII GRGCYC 6 cut(s) 372, 640, 834, 873, 1358, 1375
BauI CACGAG 1 cut(s) 1649
BbrPI CACGTG 1 cut(s) 507
BbsI GAAGAC 2 cut(s) 541, 1534
Bbv12I GWGCWC 4 cut(s) 809, 834, 873, 1032
BbvI GCAGC 4 cut(s) 305, 333, 405, 1200
BccI CCATC 6 cut(s) 429, 611, 1201, 1397, 1667, 1714
BceAI ACGGC 6 cut(s) 637, 746, 833, 1121, 1262, 1332
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 935, 969
BciT130I CCWGG 5 cut(s) 197, 313, 416, 937, 1596
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 5 cut(s) 299, 300, 366, 599, 1353
BcoDI GTCTC 3 cut(s) 297, 1015, 1280
BcuI ACTAGT 1 cut(s) 145
BfaI CTAG 4 cut(s) 146, 1250, 1487, 1725
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 846
BglI GCCNNNNNGGC 2 cut(s) 695, 1379
BisI GCNGC 7 cut(s) 319, 322, 394, 657, 729, 846, 1214
BlsI GCNGC 7 cut(s) 320, 323, 395, 658, 730, 847, 1215
Bme18I GGWCC 4 cut(s) 338, 921, 1425, 1480
BmeRI GACNNNNNGTC 1 cut(s) 146
BmeT110I CYCGRG 3 cut(s) 298, 1458, 1499
BmsI GCATC 4 cut(s) 333, 943, 965, 1332
BmtI GCTAGC 1 cut(s) 1728
BpiI GAAGAC 2 cut(s) 541, 1534
BplI GAGNNNNNCTC 2 cut(s) 519, 551
Bpu10I CCTNAGC 1 cut(s) 1515
BpuEI CTTGAG 2 cut(s) 997, 1357
BpuMI CCSGG 5 cut(s) 299, 300, 366, 599, 1353
BsaAI YACGTR 1 cut(s) 507
BsaBI GATNNNNATC 1 cut(s) 1407
BsaHI GRCGYC 5 cut(s) 519, 624, 785, 843, 1637
BsaI GGTCTC 1 cut(s) 297
BsaWI WCCGGW 3 cut(s) 966, 1037, 1465
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bse118I RCCGGY 2 cut(s) 38, 1037
Bse1I ACTGG 2 cut(s) 293, 635
Bse21I CCTNAGG 1 cut(s) 641
Bse3DI GCAATG 2 cut(s) 231, 738
Bse8I GATNNNNATC 1 cut(s) 1407
BseAI TCCGGA 1 cut(s) 1465
BseBI CCWGG 5 cut(s) 197, 313, 416, 937, 1596
BseGI GGATG 3 cut(s) 1212, 1347, 1678
BseJI GATNNNNATC 1 cut(s) 1407
BseMI GCAATG 2 cut(s) 231, 738
BseMII CTCAG 3 cut(s) 151, 655, 1529
BseNI ACTGG 2 cut(s) 293, 635
BseRI GAGGAG 4 cut(s) 58, 718, 764, 1298
BseSI GKGCMC 5 cut(s) 372, 640, 1358, 1375, 1419
BseXI GCAGC 4 cut(s) 305, 333, 405, 1200
BseYI CCCAGC 2 cut(s) 307, 1299
BsgI GTGCAG 1 cut(s) 1199
Bsh1236I CGCG 1 cut(s) 1273
Bsh1285I CGRYCG 1 cut(s) 559
BshNI GGYRCC 1 cut(s) 842
BshTI ACCGGT 1 cut(s) 1037
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 4 cut(s) 809, 834, 873, 1032
BsiHKCI CYCGRG 3 cut(s) 298, 1458, 1499
BsiSI CCGG 9 cut(s) 39, 299, 365, 598, 967, 1038, 1352, 1466, 1478
BslFI GGGAC 6 cut(s) 324, 385, 722, 974, 1177, 1438
BsmAI GTCTC 3 cut(s) 297, 1015, 1280
BsmBI CGTCTC 1 cut(s) 1015
BsmFI GGGAC 6 cut(s) 324, 385, 722, 974, 1177, 1438
Bso31I GGTCTC 1 cut(s) 297
BsoBI CYCGRG 3 cut(s) 298, 1458, 1499
Bsp120I GGGCCC 4 cut(s) 368, 636, 1354, 1371
Bsp1286I GDGCHC 9 cut(s) 372, 640, 809, 834, 873, 1032, 1358, 1375, 1419
Bsp13I TCCGGA 1 cut(s) 1465
Bsp143I GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
Bsp19I CCATGG 1 cut(s) 1434
Bsp68I TCGCGA 1 cut(s) 1273
BspCNI CTCAG 3 cut(s) 150, 654, 1528
BspEI TCCGGA 1 cut(s) 1465
BspFNI CGCG 1 cut(s) 1273
BspMAI CTGCAG 1 cut(s) 323
BspOI GCTAGC 1 cut(s) 1728
BspPI GGATC 5 cut(s) 1231, 1457, 1470, 1560, 1582
BspQI GCTCTTC 1 cut(s) 861
BspT107I GGYRCC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 297
BsrDI GCAATG 2 cut(s) 231, 738
BsrFI RCCGGY 2 cut(s) 38, 1037
BsrI ACTGG 2 cut(s) 293, 635
BssAI RCCGGY 2 cut(s) 38, 1037
BssMI GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
BssNI GRCGYC 5 cut(s) 519, 624, 785, 843, 1637
BssSI CACGAG 1 cut(s) 1649
BssT1I CCWWGG 3 cut(s) 1099, 1434, 1751
Bst2BI CACGAG 1 cut(s) 1649
Bst2UI CCWGG 5 cut(s) 197, 313, 416, 937, 1596
Bst4CI ACNGT 5 cut(s) 857, 1174, 1569, 1621, 1668
Bst6I CTCTTC 2 cut(s) 671, 861
BstACI GRCGYC 5 cut(s) 519, 624, 785, 843, 1637
BstAPI GCANNNNNTGC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 507
BstC8I GCNNGC 9 cut(s) 238, 242, 380, 636, 783, 1275, 1419, 1563, 1726
BstDEI CTNAG 5 cut(s) 137, 641, 1515, 1556, 1703
BstDSI CCRYGG 3 cut(s) 849, 1173, 1434
BstEII GGTNACC 1 cut(s) 1765
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 1212, 1347, 1678
BstFNI CGCG 1 cut(s) 1273
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 3 cut(s) 465, 845, 1749
BstKTI GATC 9 cut(s) 777, 928, 1134, 1188, 1239, 1386, 1465, 1555, 1577
BstMAI GTCTC 3 cut(s) 297, 1015, 1280
BstMBI GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 5 cut(s) 237, 440, 695, 787, 1379
BstNI CCWGG 5 cut(s) 197, 313, 416, 937, 1596
BstNSI RCATGY 2 cut(s) 240, 400
BstPI GGTNACC 1 cut(s) 1765
BstSFI CTRYAG 1 cut(s) 319
BstSLI GKGCMC 5 cut(s) 372, 640, 1358, 1375, 1419
BstUI CGCG 1 cut(s) 1273
BstV1I GCAGC 4 cut(s) 305, 333, 405, 1200
BstV2I GAAGAC 2 cut(s) 541, 1534
BstX2I RGATCY 2 cut(s) 1462, 1552
BstXI CCANNNNNNTGG 1 cut(s) 1435
BstYI RGATCY 2 cut(s) 1462, 1552
Bsu36I CCTNAGG 1 cut(s) 641
BtgI CCRYGG 3 cut(s) 849, 1173, 1434
BtsCI GGATG 3 cut(s) 1212, 1347, 1678
BtsI GCAGTG 2 cut(s) 90, 438
BtsIMutI CAGTG 5 cut(s) 90, 438, 862, 1386, 1617
BtuMI TCGCGA 1 cut(s) 1273
Cac8I GCNNGC 9 cut(s) 238, 242, 380, 636, 783, 1275, 1419, 1563, 1726
CfoI GCGC 3 cut(s) 465, 845, 1749
Cfr10I RCCGGY 2 cut(s) 38, 1037
Cfr9I CCCGGG 1 cut(s) 298
CseI GACGC 3 cut(s) 613, 774, 1626
Csp6I GTAC 1 cut(s) 826
CspAI ACCGGT 1 cut(s) 1037
CviQI GTAC 1 cut(s) 826
DdeI CTNAG 5 cut(s) 137, 641, 1515, 1556, 1703
DinI GGCGCC 1 cut(s) 844
DpnI GATC 9 cut(s) 776, 927, 1133, 1187, 1238, 1385, 1464, 1554, 1576
DpnII GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
DrdI GACNNNNNNGTC 2 cut(s) 563, 860
DriI GACNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 2 cut(s) 563, 860
EaeI YGGCCR 4 cut(s) 484, 759, 942, 1475
Eam1104I CTCTTC 2 cut(s) 671, 861
Eam1105I GACNNNNNGTC 1 cut(s) 146
EarI CTCTTC 2 cut(s) 671, 861
EciI GGCGGA 3 cut(s) 565, 643, 1600
Ecl136II GAGCTC 2 cut(s) 832, 871
Eco130I CCWWGG 3 cut(s) 1099, 1434, 1751
Eco147I AGGCCT 2 cut(s) 378, 689
Eco24I GRGCYC 6 cut(s) 372, 640, 834, 873, 1358, 1375
Eco31I GGTCTC 1 cut(s) 297
Eco32I GATATC 2 cut(s) 1294, 1409
Eco47I GGWCC 4 cut(s) 338, 921, 1425, 1480
Eco53kI GAGCTC 2 cut(s) 832, 871
Eco57I CTGAAG 1 cut(s) 460
Eco72I CACGTG 1 cut(s) 507
Eco81I CCTNAGG 1 cut(s) 641
Eco88I CYCGRG 3 cut(s) 298, 1458, 1499
Eco91I GGTNACC 1 cut(s) 1765
EcoICRI GAGCTC 2 cut(s) 832, 871
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoO109I RGGNCCY 3 cut(s) 368, 637, 1066
EcoO65I GGTNACC 1 cut(s) 1765
EcoRII CCWGG 5 cut(s) 195, 311, 414, 935, 1594
EcoRV GATATC 2 cut(s) 1294, 1409
EcoT14I CCWWGG 3 cut(s) 1099, 1434, 1751
EcoT38I GRGCYC 6 cut(s) 372, 640, 834, 873, 1358, 1375
EgeI GGCGCC 1 cut(s) 844
EheI GGCGCC 1 cut(s) 844
ErhI CCWWGG 3 cut(s) 1099, 1434, 1751
Esp3I CGTCTC 1 cut(s) 1015
FalI AAGNNNNNCTT 2 cut(s) 1319, 1351
FaqI GGGAC 6 cut(s) 324, 385, 722, 974, 1177, 1438
FauI CCCGC 3 cut(s) 627, 774, 1426
FbaI TGATCA 1 cut(s) 774
FblI GTMKAC 3 cut(s) 627, 885, 1080
Fnu4HI GCNGC 7 cut(s) 319, 322, 394, 657, 729, 846, 1214
FokI GGATG 3 cut(s) 1219, 1354, 1685
FriOI GRGCYC 6 cut(s) 372, 640, 834, 873, 1358, 1375
Fsp4HI GCNGC 7 cut(s) 319, 322, 394, 657, 729, 846, 1214
FspBI CTAG 4 cut(s) 146, 1250, 1487, 1725
GlaI GCGC 3 cut(s) 464, 844, 1748
GluI GCNGC 7 cut(s) 319, 322, 394, 657, 729, 846, 1214
GsaI CCCAGC 2 cut(s) 311, 1303
HaeII RGCGCY 1 cut(s) 846
HapII CCGG 9 cut(s) 39, 299, 365, 598, 967, 1038, 1352, 1466, 1478
HgaI GACGC 3 cut(s) 613, 774, 1626
HhaI GCGC 3 cut(s) 465, 845, 1749
Hin1I GRCGYC 5 cut(s) 519, 624, 785, 843, 1637
Hin6I GCGC 3 cut(s) 463, 843, 1747
HinP1I GCGC 3 cut(s) 463, 843, 1747
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HinfI GANTC 3 cut(s) 495, 572, 1195
HpaII CCGG 9 cut(s) 39, 299, 365, 598, 967, 1038, 1352, 1466, 1478
HphI GGTGA 3 cut(s) 287, 1336, 1450
Hpy166II GTNNAC 5 cut(s) 628, 853, 886, 1081, 1687
Hpy188III TCNNGA 8 cut(s) 224, 739, 1014, 1272, 1336, 1460, 1466, 1517
Hpy8I GTNNAC 5 cut(s) 628, 853, 886, 1081, 1687
HpyAV CCTTC 8 cut(s) 22, 87, 226, 484, 884, 1260, 1321, 1514
HpyCH4III ACNGT 5 cut(s) 857, 1174, 1569, 1621, 1668
HpyCH4IV ACGT 5 cut(s) 506, 519, 771, 1003, 1083
HpyCH4V TGCA 9 cut(s) 4, 95, 210, 236, 240, 321, 1216, 1345, 1565
HpyF10VI GCNNNNNNNGC 5 cut(s) 237, 440, 695, 787, 1379
HpyF3I CTNAG 5 cut(s) 137, 641, 1515, 1556, 1703
HpySE526I ACGT 5 cut(s) 506, 519, 771, 1003, 1083
Hsp92I GRCGYC 5 cut(s) 519, 624, 785, 843, 1637
HspAI GCGC 3 cut(s) 463, 843, 1747
KasI GGCGCC 1 cut(s) 842
Kpn2I TCCGGA 1 cut(s) 1465
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 3 cut(s) 548, 741, 983
Lsp1109I GCAGC 4 cut(s) 305, 333, 405, 1200
LweI GCATC 4 cut(s) 333, 943, 965, 1332
MaeI CTAG 4 cut(s) 146, 1250, 1487, 1725
MaeII ACGT 5 cut(s) 506, 519, 771, 1003, 1083
MaeIII GTNAC 4 cut(s) 502, 767, 1621, 1765
MalI GATC 9 cut(s) 776, 927, 1133, 1187, 1238, 1385, 1464, 1554, 1576
MboI GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
MboII GAAGA 6 cut(s) 541, 658, 878, 923, 1079, 1534
MflI RGATCY 2 cut(s) 1462, 1552
MhlI GDGCHC 9 cut(s) 372, 640, 809, 834, 873, 1032, 1358, 1375, 1419
MluCI AATT 6 cut(s) 282, 360, 755, 916, 1017, 1398
Mly113I GGCGCC 1 cut(s) 843
MlyI GAGTC 1 cut(s) 504
MmeI TCCRAC 4 cut(s) 1143, 1243, 1530, 1650
MroI TCCGGA 1 cut(s) 1465
MroXI GAANNNNTTC 2 cut(s) 1004, 1021
MseI TTAA 2 cut(s) 914, 1494
MslI CAYNNNNRTG 3 cut(s) 1127, 1433, 1653
MspA1I CMGCKG 1 cut(s) 1303
MspI CCGG 9 cut(s) 39, 299, 365, 598, 967, 1038, 1352, 1466, 1478
MvaI CCWGG 5 cut(s) 197, 313, 416, 937, 1596
MvnI CGCG 1 cut(s) 1273
MwoI GCNNNNNNNGC 5 cut(s) 237, 440, 695, 787, 1379
NarI GGCGCC 1 cut(s) 843
NciI CCSGG 5 cut(s) 299, 300, 366, 599, 1353
NcoI CCATGG 1 cut(s) 1434
NdeII GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
NheI GCTAGC 1 cut(s) 1724
NmeAIII GCCGAG 1 cut(s) 462
NmuCI GTSAC 2 cut(s) 502, 1621
NruI TCGCGA 1 cut(s) 1273
NspI RCATGY 2 cut(s) 240, 400
PaeI GCATGC 1 cut(s) 240
PceI AGGCCT 2 cut(s) 378, 689
PciSI GCTCTTC 1 cut(s) 861
PcsI WCGNNNNNNNCGW 3 cut(s) 744, 846, 894
PdmI GAANNNNTTC 2 cut(s) 1004, 1021
PfeI GAWTC 2 cut(s) 572, 1195
PflFI GACNNNGTC 1 cut(s) 1076
PinAI ACCGGT 1 cut(s) 1037
PkrI GCNGC 7 cut(s) 320, 323, 395, 658, 730, 847, 1215
PleI GAGTC 1 cut(s) 503
PluTI GGCGCC 1 cut(s) 846
PmaCI CACGTG 1 cut(s) 507
PmlI CACGTG 1 cut(s) 507
PpsI GAGTC 1 cut(s) 503
Ppu21I YACGTR 1 cut(s) 507
Psp124BI GAGCTC 2 cut(s) 834, 873
Psp1406I AACGTT 1 cut(s) 1003
Psp6I CCWGG 5 cut(s) 195, 311, 414, 935, 1594
PspCI CACGTG 1 cut(s) 507
PspEI GGTNACC 1 cut(s) 1765
PspFI CCCAGC 2 cut(s) 307, 1299
PspGI CCWGG 5 cut(s) 195, 311, 414, 935, 1594
PspOMI GGGCCC 4 cut(s) 368, 636, 1354, 1371
PstI CTGCAG 1 cut(s) 323
PsuI RGATCY 2 cut(s) 1462, 1552
PsyI GACNNNGTC 1 cut(s) 1076
PvuII CAGCTG 1 cut(s) 1303
RruI TCGCGA 1 cut(s) 1273
RsaI GTAC 1 cut(s) 827
RsaNI GTAC 1 cut(s) 826
RseI CAYNNNNRTG 3 cut(s) 1127, 1433, 1653
SacI GAGCTC 2 cut(s) 834, 873
SalI GTCGAC 1 cut(s) 1079
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 2 cut(s) 914, 1494
SatI GCNGC 7 cut(s) 319, 322, 394, 657, 729, 846, 1214
Sau3AI GATC 9 cut(s) 774, 925, 1131, 1185, 1236, 1383, 1462, 1552, 1574
SchI GAGTC 1 cut(s) 504
SduI GDGCHC 9 cut(s) 372, 640, 809, 834, 873, 1032, 1358, 1375, 1419
SfaNI GCATC 4 cut(s) 333, 943, 965, 1332
SfcI CTRYAG 1 cut(s) 319
SfiI GGCCNNNNNGGCC 1 cut(s) 695
SfoI GGCGCC 1 cut(s) 844
SinI GGWCC 4 cut(s) 338, 921, 1425, 1480
SmaI CCCGGG 1 cut(s) 300
SmiMI CAYNNNNRTG 3 cut(s) 1127, 1433, 1653
SmlI CTYRAG 2 cut(s) 1012, 1336
SmoI CTYRAG 2 cut(s) 1012, 1336
SpeI ACTAGT 1 cut(s) 145
SphI GCATGC 1 cut(s) 240
Sse9I AATT 6 cut(s) 282, 360, 755, 916, 1017, 1398
SseBI AGGCCT 2 cut(s) 378, 689
SspDI GGCGCC 1 cut(s) 842
SspMI CTAG 4 cut(s) 146, 1250, 1487, 1725
SstI GAGCTC 2 cut(s) 834, 873
StuI AGGCCT 2 cut(s) 378, 689
StyI CCWWGG 3 cut(s) 1099, 1434, 1751
TaaI ACNGT 5 cut(s) 857, 1174, 1569, 1621, 1668
TaiI ACGT 5 cut(s) 509, 522, 774, 1006, 1086
TaqI TCGA 6 cut(s) 834, 864, 1080, 1188, 1232, 1281
TaqII GACCGA 2 cut(s) 326, 1528
TasI AATT 6 cut(s) 282, 360, 755, 916, 1017, 1398
TauI GCSGC 3 cut(s) 659, 731, 848
TfiI GAWTC 2 cut(s) 572, 1195
Tru1I TTAA 2 cut(s) 914, 1494
Tru9I TTAA 2 cut(s) 914, 1494
TscAI CASTG 5 cut(s) 97, 445, 862, 1393, 1624
TseFI GTSAC 2 cut(s) 502, 1621
TseI GCWGC 4 cut(s) 318, 321, 393, 1213
Tsp45I GTSAC 2 cut(s) 502, 1621
TspDTI ATGAA 2 cut(s) 170, 1074
TspGWI ACGGA 2 cut(s) 535, 1181
TspMI CCCGGG 1 cut(s) 298
TspRI CASTG 5 cut(s) 97, 445, 862, 1393, 1624
Tth111I GACNNNGTC 1 cut(s) 1076
VpaK11BI GGWCC 4 cut(s) 338, 921, 1425, 1480
XagI CCTNNNNNAGG 1 cut(s) 387
XceI RCATGY 2 cut(s) 240, 400
XcmI CCANNNNNNNNNTGG 2 cut(s) 466, 1365
XmaI CCCGGG 1 cut(s) 298
XmiI GTMKAC 3 cut(s) 627, 885, 1080
XmnI GAANNNNTTC 2 cut(s) 1004, 1021
XspI CTAG 4 cut(s) 146, 1250, 1487, 1725
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.