MD01G1052000.v1.1

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016388
Physical Location & Seq
Reverse (-)
15706605 .. 15708279
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1052000.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGCCTAGCCCTAGCCCTACCGCTAGCAATACCAACACAAACCCTAATTCTCCTAAAGAAGGAAACGGATTAGTGATCGTGAGAGAAAAAGGCCATATATTGGAGAGTTCTAGTGGTCAGGTTGGGTCTGGAAGTCATGAGGAAGAAGAAAAGTCAGGGAACCAACAAGAAGAAGAACATCAACCTCTACTTCCCAATAAGAAATGCTCCCATTCGAACATCCAAGAAATCCCAGATTTACTTGAAGAATGTTGTCCACATCCTAAGAAACCAAGGTCGCCCACTAAAGCTAAAAGTATTATCTTGTTCGGAAAAGAGTTAACTATAGCAACCGACATCCCTGTCAGTACTAGAGCTAGTGGTACTGAACTTGATTATGTTCATGAAGCACAAACAATGATTGGTGATAAGGATAATTTGAAAAGAAAATACATCGACGTTGATGATGGTGAAGATCATGTGGGGCCTGCACATCTGAATGAGAAGAAGAAGAAGGTCAAGTTTGTCTTCAACAACAACTTGAACGCAACAAATAAGGTGGTGAAGAAGGGTAAAAATAAGGTAAAATTTGCTAGTGGTATGCCAGCCCCTGACTTGCCTGAAGAATTCAAGAGCTTGATCTTAGGAGAAATGAATGGGACAAAGCTGCAAATGTTGGCACAAAAGAGTTTGTACAACACTGACATAAACAAAGACCAAGGCAGGTTGTCACTGCCTCGAATGCAAACCGAATCCAAGAATTTTCTCAAACCCAACGAAAAGGAGGAACTAGATAAAACTAGCCTCCTGATGGTTCCTTTGATTGGCCCAAAGCTTGAACCTGACAAGGTATCCCTAGGATTGTGGACAATGTCAAGCAAATCTCAAAAAACCTATGTGCTGAAATCTACATGGTTTGGCATTGTTGAAAGACATAGTCTAGGTGTAGGTGATGTGGTCCAAGTTTGGTCATTTCGGGCTACAAATCCTAAGTACAAAATTAAGGATGGTGATCATGGTGATCACGATCACTTGACTTGCAACGATGATCAGCTTCACTTAGCTCTTGTCTTGGTTGAGAGAGGCGATCGTCTTCTCGAGGAAGGCGGTAGCAAAGAAGGTGGTACTAGTACTGGTGGAGTAAGTAGTAGTATGAGCAATAATGAGTGTGATGAAGGACGAAAAACAATTGATGGCAGCAGCAGTGGGAGCTGCACTCCTGAAAATGATGGGAATAGTAGCCCCAAACCGAGTTGTGAAGAGAGAGACCAAGAGACTTTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

422

Amino Acids

46.28

Weight (kDa)

5.96

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 207 - 303 1.4e-11 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016388)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g13010
malus_domestica MD01G1052000.v1.1
prunus_persica Prupe.2G154200_v2.0.a1
rosa_laevigata RLG00000008091 RLG00000028355
rosa_multiflora Rmu_sc0001599.1_g000013
rosa_roxburghii Rroxscaffold_5G00358980
rosa_rugosa Rorug01G0225200 Rorug04G0132500
rosa_samantha Rh1AG239400 Rh1BG210200 Rh1DG236900 Rh4AG192600 Rh4DG190500
rosa_wichuraiana Rw1G020780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
Acc36I ACCTGC 1 cut(s) 693
AccB7I CCANNNNNTGG 1 cut(s) 100
AciI CCGC 2 cut(s) 21, 1086
AcsI RAATTY 3 cut(s) 566, 605, 739
AcuI CTGAAG 1 cut(s) 621
AfaI GTAC 6 cut(s) 349, 364, 674, 974, 1105, 1111
AfiI CCNNNNNNNGG 4 cut(s) 59, 100, 790, 803
AgsI TTSAA 7 cut(s) 245, 421, 511, 523, 610, 818, 908
AhlI ACTAGT 1 cut(s) 1106
AluBI AGCT 8 cut(s) 290, 356, 615, 646, 814, 1033, 1043, 1191
AluI AGCT 8 cut(s) 290, 356, 615, 646, 814, 1033, 1043, 1191
Alw26I GTCTC 2 cut(s) 1239, 1247
AlwNI CAGNNNCTG 1 cut(s) 590
Ama87I CYCGRG 1 cut(s) 1076
AoxI GGCC 3 cut(s) 91, 464, 805
ApeKI GCWGC 4 cut(s) 646, 1176, 1179, 1191
ApoI RAATTY 3 cut(s) 566, 605, 739
AspA2I CCTAGG 1 cut(s) 835
AspS9I GGNCC 3 cut(s) 464, 806, 937
AsuHPI GGTGA 6 cut(s) 416, 461, 553, 941, 1001, 1010
AsuII TTCGAA 1 cut(s) 215
AsuNHI GCTAGC 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 1076
AvaII GGWCC 1 cut(s) 937
AvrII CCTAGG 1 cut(s) 835
BbsI GAAGAC 2 cut(s) 499, 1064
BbvI GCAGC 4 cut(s) 633, 1178, 1188, 1191
BccI CCATC 5 cut(s) 440, 784, 980, 1166, 1202
BciVI GTATCC 1 cut(s) 841
BclI TGATCA 3 cut(s) 991, 1000, 1027
BcoDI GTCTC 2 cut(s) 1239, 1247
BcuI ACTAGT 1 cut(s) 1106
BfmI CTRYAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 693
BfuI GTATCC 1 cut(s) 841
BisI GCNGC 4 cut(s) 647, 1177, 1180, 1192
BlnI CCTAGG 1 cut(s) 835
BlsI GCNGC 4 cut(s) 648, 1178, 1181, 1193
BmcAI AGTACT 2 cut(s) 349, 1111
Bme18I GGWCC 1 cut(s) 937
BmeT110I CYCGRG 1 cut(s) 1076
BmgT120I GGNCC 3 cut(s) 464, 806, 937
BmiI GGNNCC 3 cut(s) 161, 465, 795
BmtI GCTAGC 1 cut(s) 27
BpiI GAAGAC 2 cut(s) 499, 1064
BplI GAGNNNNNCTC 2 cut(s) 1180, 1212
Bpu14I TTCGAA 1 cut(s) 215
BsaBI GATNNNNATC 2 cut(s) 990, 1005
BsaI GGTCTC 1 cut(s) 1239
BsaJI CCNNGG 3 cut(s) 272, 697, 835
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 100, 790, 803
Bse1I ACTGG 1 cut(s) 1117
Bse8I GATNNNNATC 2 cut(s) 990, 1005
BseDI CCNNGG 3 cut(s) 272, 697, 835
BseGI GGATG 4 cut(s) 219, 259, 336, 991
BseJI GATNNNNATC 2 cut(s) 990, 1005
BseLI CCNNNNNNNGG 4 cut(s) 59, 100, 790, 803
BseNI ACTGG 1 cut(s) 1117
BseXI GCAGC 4 cut(s) 633, 1178, 1188, 1191
BsgI GTGCAG 2 cut(s) 453, 1177
Bsh1285I CGRYCG 1 cut(s) 1069
BshFI GGCC 3 cut(s) 93, 466, 807
BsiEI CGRYCG 1 cut(s) 1069
BsiHKCI CYCGRG 1 cut(s) 1076
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 4 cut(s) 59, 100, 790, 803
BsmAI GTCTC 2 cut(s) 1239, 1247
BsmFI GGGAC 1 cut(s) 652
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 3 cut(s) 93, 466, 807
Bso31I GGTCTC 1 cut(s) 1239
BsoBI CYCGRG 1 cut(s) 1076
Bsp119I TTCGAA 1 cut(s) 215
Bsp1407I TGTACA 1 cut(s) 672
Bsp143I GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
BspACI CCGC 2 cut(s) 21, 1086
BspANI GGCC 3 cut(s) 93, 466, 807
BspHI TCATGA 2 cut(s) 136, 382
BspLI GGNNCC 3 cut(s) 161, 465, 795
BspMI ACCTGC 1 cut(s) 693
BspOI GCTAGC 1 cut(s) 27
BspT104I TTCGAA 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 1239
BsrGI TGTACA 1 cut(s) 672
BsrI ACTGG 1 cut(s) 1117
BssECI CCNNGG 3 cut(s) 272, 697, 835
BssMI GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
BssT1I CCWWGG 3 cut(s) 272, 697, 835
Bst6I CTCTTC 1 cut(s) 1233
BstAUI TGTACA 1 cut(s) 672
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 25, 468, 585
BstDEI CTNAG 4 cut(s) 264, 622, 969, 1039
BstENI CCTNNNNNAGG 1 cut(s) 57
BstF5I GGATG 4 cut(s) 219, 259, 336, 991
BstKTI GATC 8 cut(s) 78, 457, 621, 994, 1003, 1009, 1030, 1069
BstMAI GTCTC 2 cut(s) 1239, 1247
BstMBI GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
BstMCI CGRYCG 1 cut(s) 1069
BstMWI GCNNNNNNNGC 2 cut(s) 721, 1188
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 4 cut(s) 633, 1178, 1188, 1191
BstV2I GAAGAC 2 cut(s) 499, 1064
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 3 cut(s) 93, 466, 807
BtsCI GGATG 4 cut(s) 219, 259, 336, 991
BtsI GCAGTG 2 cut(s) 710, 1189
BtsIMutI CAGTG 3 cut(s) 678, 710, 1189
BveI ACCTGC 1 cut(s) 693
Cac8I GCNNGC 3 cut(s) 25, 468, 585
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 2 cut(s) 136, 382
Cfr13I GGNCC 3 cut(s) 464, 806, 937
Csp6I GTAC 6 cut(s) 348, 363, 673, 973, 1104, 1110
CspCI CAANNNNNGTGG 4 cut(s) 519, 554, 1081, 1116
CviAII CATG 5 cut(s) 137, 383, 458, 891, 995
CviQI GTAC 6 cut(s) 348, 363, 673, 973, 1104, 1110
DdeI CTNAG 4 cut(s) 264, 622, 969, 1039
DpnI GATC 8 cut(s) 77, 456, 620, 993, 1002, 1008, 1029, 1068
DpnII GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 1233
EarI CTCTTC 1 cut(s) 1233
Eco130I CCWWGG 3 cut(s) 272, 697, 835
Eco31I GGTCTC 1 cut(s) 1239
Eco47I GGWCC 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 621
Eco88I CYCGRG 1 cut(s) 1076
EcoNI CCTNNNNNAGG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 464
EcoRI GAATTC 1 cut(s) 605
EcoT14I CCWWGG 3 cut(s) 272, 697, 835
ErhI CCWWGG 3 cut(s) 272, 697, 835
FaeI CATG 5 cut(s) 140, 386, 461, 894, 998
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 1 cut(s) 652
FatI CATG 5 cut(s) 136, 382, 457, 890, 994
FbaI TGATCA 3 cut(s) 991, 1000, 1027
Fnu4HI GCNGC 4 cut(s) 647, 1177, 1180, 1192
FokI GGATG 4 cut(s) 206, 246, 323, 998
Fsp4HI GCNGC 4 cut(s) 647, 1177, 1180, 1192
GluI GCNGC 4 cut(s) 647, 1177, 1180, 1192
HaeIII GGCC 3 cut(s) 93, 466, 807
Hin1II CATG 5 cut(s) 140, 386, 461, 894, 998
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HindIII AAGCTT 1 cut(s) 812
HinfI GANTC 1 cut(s) 731
HpaI GTTAAC 1 cut(s) 321
HphI GGTGA 6 cut(s) 416, 461, 553, 941, 1001, 1010
Hpy166II GTNNAC 3 cut(s) 257, 321, 846
Hpy188I TCNGA 2 cut(s) 311, 477
Hpy188III TCNNGA 9 cut(s) 79, 129, 137, 383, 610, 787, 1004, 1076, 1199
Hpy8I GTNNAC 3 cut(s) 257, 321, 846
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 6 cut(s) 53, 487, 541, 1076, 1091, 1148
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 5 cut(s) 470, 649, 724, 1020, 1194
HpyF10VI GCNNNNNNNGC 2 cut(s) 721, 1188
HpyF3I CTNAG 4 cut(s) 264, 622, 969, 1039
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 5 cut(s) 140, 386, 461, 894, 998
Ksp22I TGATCA 3 cut(s) 991, 1000, 1027
KspAI GTTAAC 1 cut(s) 321
Kzo9I GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
LmnI GCTCC 2 cut(s) 212, 1188
Lsp1109I GCAGC 4 cut(s) 633, 1178, 1188, 1191
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 1 cut(s) 708
MalI GATC 8 cut(s) 77, 456, 620, 993, 1002, 1008, 1029, 1068
MboI GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
MfeI CAATTG 1 cut(s) 1167
MluCI AATT 8 cut(s) 46, 415, 566, 605, 739, 978, 1167, 1261
MnlI CCTC 7 cut(s) 133, 195, 726, 757, 794, 1055, 1072
MseI TTAA 3 cut(s) 320, 981, 1260
MslI CAYNNNNRTG 1 cut(s) 477
MunI CAATTG 1 cut(s) 1167
Mva1269I GAATGC 1 cut(s) 726
MwoI GCNNNNNNNGC 2 cut(s) 721, 1188
NdeII GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
NheI GCTAGC 1 cut(s) 23
NlaIII CATG 5 cut(s) 140, 386, 461, 894, 998
NlaIV GGNNCC 3 cut(s) 161, 465, 795
NmuCI GTSAC 1 cut(s) 708
NspV TTCGAA 1 cut(s) 215
PaeR7I CTCGAG 1 cut(s) 1076
PagI TCATGA 2 cut(s) 136, 382
PctI GAATGC 1 cut(s) 726
PfeI GAWTC 1 cut(s) 731
PflFI GACNNNGTC 2 cut(s) 850, 915
PflMI CCANNNNNTGG 1 cut(s) 100
PkrI GCNGC 4 cut(s) 648, 1178, 1181, 1193
Ple19I CGATCG 1 cut(s) 1069
PspN4I GGNNCC 3 cut(s) 161, 465, 795
PspPI GGNCC 3 cut(s) 464, 806, 937
PstNI CAGNNNCTG 1 cut(s) 590
PsyI GACNNNGTC 2 cut(s) 850, 915
PvuI CGATCG 1 cut(s) 1069
RsaI GTAC 6 cut(s) 349, 364, 674, 974, 1105, 1111
RsaNI GTAC 6 cut(s) 348, 363, 673, 973, 1104, 1110
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 3 cut(s) 320, 981, 1260
SatI GCNGC 4 cut(s) 647, 1177, 1180, 1192
Sau3AI GATC 8 cut(s) 75, 454, 618, 991, 1000, 1006, 1027, 1066
Sau96I GGNCC 3 cut(s) 464, 806, 937
ScaI AGTACT 2 cut(s) 349, 1111
SfcI CTRYAG 1 cut(s) 324
Sfr274I CTCGAG 1 cut(s) 1076
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 1 cut(s) 937
SlaI CTCGAG 1 cut(s) 1076
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
SpeI ACTAGT 1 cut(s) 1106
Sse9I AATT 8 cut(s) 46, 415, 566, 605, 739, 978, 1167, 1261
SsiI CCGC 2 cut(s) 21, 1086
StyI CCWWGG 3 cut(s) 272, 697, 835
TaiI ACGT 1 cut(s) 441
TaqI TCGA 4 cut(s) 215, 435, 718, 1077
TasI AATT 8 cut(s) 46, 415, 566, 605, 739, 978, 1167, 1261
TatI WGTACW 4 cut(s) 347, 672, 972, 1109
TfiI GAWTC 1 cut(s) 731
Tru1I TTAA 3 cut(s) 320, 981, 1260
Tru9I TTAA 3 cut(s) 320, 981, 1260
TscAI CASTG 3 cut(s) 685, 717, 1189
TseFI GTSAC 1 cut(s) 708
TseI GCWGC 4 cut(s) 646, 1176, 1179, 1191
Tsp45I GTSAC 1 cut(s) 708
TspDTI ATGAA 4 cut(s) 371, 399, 647, 1167
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 3 cut(s) 685, 717, 1189
Tth111I GACNNNGTC 2 cut(s) 850, 915
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 937
XagI CCTNNNNNAGG 1 cut(s) 57
XapI RAATTY 3 cut(s) 566, 605, 739
XhoI CTCGAG 1 cut(s) 1076
XmaJI CCTAGG 1 cut(s) 835
ZrmI AGTACT 2 cut(s) 349, 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.