Rorug04G0132500

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001356
Physical Location & Seq
Reverse (-)
21233292 .. 21249588
16297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0132500.1

Sequence Viewer

Length: 1017 bp
ATGGCTGGGCGGCGACATATTTTACCATCCTTTGACAGACGCCCTATACAGTCTCCAGGGATGATGCGGCACGGGCCATTGCCTGGGTTAGGTCGTGCTCCTGGCCACCGAGTTTTGGAGCCAGTACCCCGTGCTGAACTACTGGAAAACAAAATTGCATATCAAGCAGCAGAGATAAAACAACTTGCAGGAGACAATCATAGACTGGCAGCTACTGAGGTGGATTTGAGGCGGGAGCTTGTAGCTGCAGAGGAGGAAGTTCAAACAGTCAAGGCACACATGAGAAGCATCCAGACTGAGAGCGACATCCAGATCCGAGCGTTGCTTGATAAGATTGTAAAAAAGGAACAGGAAATTGTAGCCGGTGAGGGTGTGAAGAAGGAATTGCAAAAGGCTCACATGGAAGCACACAGCTTGGTGGAAGCACGGAAAGAGCTAAGAATTCAAATTCGACAGGCCACTGATGAGTTGCAGAAAGCTTGTATTGAGGTTAAGAAACTGCCAGATCTGCACGGTGAACTTGATAGTTTGAGACAAGAACACGAGAGGCTGCGTGCTACTTTTGAATATGAAAAAGGTAGAAACATAGAACAAGTCGAGCAAATGAAAGCAATGGAGAAGAATCTAATGGGCATGGCAGGAGAAGTAGAAAGGTTGCGTGCTGAGGTCTTGAATGCAGAGAAGAGAGCACAAGCACCAAATCCACGTAATGGCGGTTACATGAATCCAGATTCTTATTACACTGCTACCATGCCAGGTGCTAGTGCGTATTTTGATGGTTATGGAAGGCCTTATGTTTCAATGGGTAATAGGCCACTGGGAGAGGGAATAATTCAATTTGCCAGCAGCAGCAAGCAGCATGCTGCTGCAGGTATTCCAACTGCTGGGGATGGTGCTATCTGGGGAGCAGCATATAATCCCTTGCTTGGCACTGCTTCTGGTGGTGCTACTGTCCCTACCACTGGGAGCAGTGCTGTTTGGGGAGGAGCATATGATTCCGCACTTGCTCGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

338

Amino Acids

37.1

Weight (kDa)

7.86

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LAMB4 PF24999 137 - 187 2.3e-06 Laminin subunit beta-4-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 860
AccB7I CCANNNNNTGG 3 cut(s) 83, 710, 884
AciI CCGC 5 cut(s) 10, 67, 232, 714, 999
AclWI GGATC 1 cut(s) 307
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 441, 447
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 884, 926, 962
AgsI TTSAA 6 cut(s) 263, 446, 566, 673, 801, 836
AjnI CCWGG 4 cut(s) 55, 82, 100, 754
AluBI AGCT 6 cut(s) 212, 238, 245, 414, 436, 479
AluI AGCT 6 cut(s) 212, 238, 245, 414, 436, 479
Alw21I GWGCWC 2 cut(s) 100, 691
Alw26I GTCTC 3 cut(s) 57, 186, 526
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 1 cut(s) 215
AoxI GGCC 5 cut(s) 74, 103, 456, 788, 812
ApoI RAATTY 2 cut(s) 441, 447
AspS9I GGNCC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 377, 527
BalI TGGCCA 1 cut(s) 105
BauI CACGAG 1 cut(s) 542
Bbv12I GWGCWC 2 cut(s) 100, 691
BbvCI CCTCAGC 1 cut(s) 663
BccI CCATC 3 cut(s) 34, 770, 884
BciT130I CCWGG 4 cut(s) 57, 84, 102, 756
BcoDI GTCTC 3 cut(s) 57, 186, 526
BfaI CTAG 1 cut(s) 762
BfmI CTRYAG 2 cut(s) 246, 867
BfuAI ACCTGC 1 cut(s) 860
BglII AGATCT 1 cut(s) 505
Bme1390I CCNGG 4 cut(s) 57, 84, 102, 756
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 4 cut(s) 57, 84, 102, 756
BmrI ACTGGG 2 cut(s) 827, 972
BmsI GCATC 2 cut(s) 54, 297
BmuI ACTGGG 2 cut(s) 827, 972
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 663
BsaAI YACGTR 1 cut(s) 707
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 56, 83
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 884, 926, 962
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 5 cut(s) 122, 147, 210, 822, 967
Bse3DI GCAATG 2 cut(s) 77, 618
BseBI CCWGG 4 cut(s) 57, 84, 102, 756
BseDI CCNNGG 2 cut(s) 56, 83
BseGI GGATG 5 cut(s) 26, 66, 288, 306, 895
BseLI CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 884, 926, 962
BseMI GCAATG 2 cut(s) 77, 618
BseMII CTCAG 3 cut(s) 207, 288, 654
BseNI ACTGG 5 cut(s) 122, 147, 210, 822, 967
BseRI GAGGAG 2 cut(s) 266, 999
BseYI CCCAGC 2 cut(s) 5, 884
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 5 cut(s) 76, 105, 458, 790, 814
BsiHKAI GWGCWC 2 cut(s) 100, 691
BsiSI CCGG 1 cut(s) 363
BslFI GGGAC 1 cut(s) 938
BslI CCNNNNNNNGG 7 cut(s) 83, 89, 115, 710, 884, 926, 962
BsmAI GTCTC 3 cut(s) 57, 186, 526
BsmFI GGGAC 1 cut(s) 938
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 5 cut(s) 76, 105, 458, 790, 814
Bsp1286I GDGCHC 2 cut(s) 100, 691
Bsp143I GATC 2 cut(s) 312, 505
BspACI CCGC 5 cut(s) 10, 67, 232, 714, 999
BspANI GGCC 5 cut(s) 76, 105, 458, 790, 814
BspCNI CTCAG 3 cut(s) 208, 289, 655
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 2 cut(s) 250, 871
BspMI ACCTGC 1 cut(s) 860
BspPI GGATC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 77, 618
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 5 cut(s) 122, 147, 210, 822, 967
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 2 cut(s) 56, 83
BssMI GATC 2 cut(s) 312, 505
BssNI GRCGYC 1 cut(s) 40
BssSI CACGAG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 4 cut(s) 57, 84, 102, 756
Bst4CI ACNGT 4 cut(s) 51, 268, 515, 952
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 1 cut(s) 40
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 5 cut(s) 555, 660, 844, 854, 861
BstDEI CTNAG 4 cut(s) 216, 297, 437, 663
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 5 cut(s) 26, 66, 288, 306, 895
BstKTI GATC 2 cut(s) 315, 508
BstMAI GTCTC 3 cut(s) 57, 186, 526
BstMBI GATC 2 cut(s) 312, 505
BstMWI GCNNNNNNNGC 3 cut(s) 73, 164, 508
BstNI CCWGG 4 cut(s) 57, 84, 102, 756
BstNSI RCATGY 1 cut(s) 863
BstSCI CCNGG 4 cut(s) 55, 82, 100, 754
BstSFI CTRYAG 2 cut(s) 246, 867
BstX2I RGATCY 2 cut(s) 312, 505
BstYI RGATCY 2 cut(s) 312, 505
BsuRI GGCC 5 cut(s) 76, 105, 458, 790, 814
BtsCI GGATG 5 cut(s) 26, 66, 288, 306, 895
BtsI GCAGTG 3 cut(s) 741, 930, 976
BtsIMutI CAGTG 6 cut(s) 459, 741, 815, 930, 960, 976
BveI ACCTGC 1 cut(s) 860
Cac8I GCNNGC 5 cut(s) 555, 660, 844, 854, 861
CaiI CAGNNNCTG 1 cut(s) 215
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 74
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 125
CviAII CATG 6 cut(s) 280, 400, 634, 721, 751, 860
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 4 cut(s) 216, 297, 437, 663
DpnI GATC 2 cut(s) 314, 507
DpnII GATC 2 cut(s) 312, 505
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
Eco147I AGGCCT 1 cut(s) 790
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 441
EcoRII CCWGG 4 cut(s) 55, 82, 100, 754
FaeI CATG 6 cut(s) 283, 403, 637, 724, 754, 863
FaqI GGGAC 1 cut(s) 938
FatI CATG 6 cut(s) 279, 399, 633, 720, 750, 859
FauI CCCGC 1 cut(s) 225
FauNDI CATATG 1 cut(s) 991
FokI GGATG 5 cut(s) 13, 73, 275, 293, 902
FspBI CTAG 1 cut(s) 762
GsaI CCCAGC 2 cut(s) 9, 888
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 5 cut(s) 76, 105, 458, 790, 814
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 6 cut(s) 283, 403, 637, 724, 754, 863
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 4 cut(s) 622, 724, 731, 995
HpaII CCGG 1 cut(s) 363
HphI GGTGA 2 cut(s) 377, 527
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 4 cut(s) 292, 310, 670, 728
Hpy8I GTNNAC 1 cut(s) 518
HpyAV CCTTC 2 cut(s) 373, 780
HpyCH4III ACNGT 4 cut(s) 51, 268, 515, 952
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 8 cut(s) 158, 188, 248, 388, 472, 511, 677, 869
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 164, 508
HpyF3I CTNAG 4 cut(s) 216, 297, 437, 663
HpySE526I ACGT 1 cut(s) 706
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 6 cut(s) 283, 403, 637, 724, 754, 863
Kzo9I GATC 2 cut(s) 312, 505
LmnI GCTCC 6 cut(s) 103, 118, 235, 905, 966, 986
LweI GCATC 2 cut(s) 54, 297
MaeI CTAG 1 cut(s) 762
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 1 cut(s) 716
MalI GATC 2 cut(s) 314, 507
MboI GATC 2 cut(s) 312, 505
MboII GAAGA 3 cut(s) 388, 631, 694
MflI RGATCY 2 cut(s) 312, 505
MhlI GDGCHC 2 cut(s) 100, 691
MlsI TGGCCA 1 cut(s) 105
MluCI AATT 7 cut(s) 153, 354, 383, 441, 447, 831, 836
MluNI TGGCCA 1 cut(s) 105
MmeI TCCRAC 1 cut(s) 902
Mox20I TGGCCA 1 cut(s) 105
MscI TGGCCA 1 cut(s) 105
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 1 cut(s) 105
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 4 cut(s) 57, 84, 102, 756
Mva1269I GAATGC 1 cut(s) 679
MvaI CCWGG 4 cut(s) 57, 84, 102, 756
MwoI GCNNNNNNNGC 3 cut(s) 73, 164, 508
NdeI CATATG 1 cut(s) 991
NdeII GATC 2 cut(s) 312, 505
NlaIII CATG 6 cut(s) 283, 403, 637, 724, 754, 863
NlaIV GGNNCC 1 cut(s) 120
NspI RCATGY 1 cut(s) 863
PaeI GCATGC 1 cut(s) 863
PceI AGGCCT 1 cut(s) 790
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 4 cut(s) 622, 724, 731, 995
PflMI CCANNNNNTGG 3 cut(s) 83, 710, 884
Ppu21I YACGTR 1 cut(s) 707
Psp6I CCWGG 4 cut(s) 55, 82, 100, 754
PspFI CCCAGC 2 cut(s) 5, 884
PspGI CCWGG 4 cut(s) 55, 82, 100, 754
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 2 cut(s) 250, 871
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 2 cut(s) 312, 505
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 492
Sau3AI GATC 2 cut(s) 312, 505
Sau96I GGNCC 1 cut(s) 74
ScrFI CCNGG 4 cut(s) 57, 84, 102, 756
SduI GDGCHC 2 cut(s) 100, 691
SfaNI GCATC 2 cut(s) 54, 297
SfcI CTRYAG 2 cut(s) 246, 867
SphI GCATGC 1 cut(s) 863
Sse9I AATT 7 cut(s) 153, 354, 383, 441, 447, 831, 836
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 5 cut(s) 10, 67, 232, 714, 999
SspMI CTAG 1 cut(s) 762
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 4 cut(s) 55, 82, 100, 754
TaaI ACNGT 4 cut(s) 51, 268, 515, 952
TaiI ACGT 1 cut(s) 709
TaqI TCGA 2 cut(s) 451, 597
TasI AATT 7 cut(s) 153, 354, 383, 441, 447, 831, 836
TauI GCSGC 2 cut(s) 13, 70
TfiI GAWTC 4 cut(s) 622, 724, 731, 995
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 6 cut(s) 466, 748, 822, 937, 967, 976
TspDTI ATGAA 3 cut(s) 585, 620, 737
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 6 cut(s) 466, 748, 822, 937, 967, 976
Van91I CCANNNNNTGG 3 cut(s) 83, 710, 884
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 2 cut(s) 441, 447
XceI RCATGY 1 cut(s) 863
XspI CTAG 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.