Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005728

Physical Location & Seq

Genomic Coordinates Forward (+)

16504512 .. 16510997

Gene Structure 6486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1061400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGGCTGTAGTGAGTTCTGTGCCTGCAATTGGTCAACTGGCAGGGCGGATAAGCTACACTCATATATCAGAATACATGGCTCGCGGAGAGATTGTGGAGGAGCCGGTGGCAGTGCCGGTGGCGGTGCCAGCGGAGCCACGGATGATCGAAGATATGATCTATGTTGCAGTGGGAAAAGACGTTAAAGAAAGCAAATCAACTCTGGAGTGGGCGGTACATAATTCTGGAGGGAAGAAAATCTGCTTAGTTCATGTTCACCAGCCTGCTCAGATGATTCCTTTGAGTTTGATGGGGACGAAGTTCCCAGCAAGCTCACTCACGAACCAGCAAGTCAGAGTGCACAGAGAAACTGAAAGGCAACACATGAATAAGATCCTTGATGATTACATTCGTATTTGTCTCCAAATGGGGGTTCGGGTAGAAAAGCTACATATTGAAATGGACTGTATTGAGAAGGGAATTGTGGAACTCATCTCTCAGCATGGGATCAGGAAACTTGTTATGGGAGCAGCAGCAGACAAGCACCATTCAAAGAAAATGATTGACCTCAAGTCCAAGAAAGCCAAATATGTCCGCCAACAAGCCCCTGTTTCCTGTCATATACAATTTATTTGCAAGGGGCACCCTATACGCACCAGGGAAGGAAATTCAGATGGAGTTGATACAGAGGTTCCATTGCTGCGACCAAGTCCAATGGAACAGCCACCGCACCCCTTTAGATCACAATCTGATACAATGGAGCGGACTAATCGAGTGAAACTCACCAATCCAGCTCAAGATTTCTTCCGCAGGGCAAGCTCGGTAAATATGCAGAAATATGGAGGCGGGACAACAGATGCTACTTCCACAGATGGTACTGAAGCGTTTTCAACTCCAAGAAATAGGTTCGAACCGGGAGCATGTCCTGAGGAATTAGATCAGGTAATAAGGAGGAGTGTTTCAGGATATTCAACAGGCTCTTCTACTGGATTGGGTGATTTCACTTTGATTCATTGTGATAGAGTTGAGGGAAGTGAAAATGGGTCAAGTAACTCATGCAGACTTCCTCATTCTAAAGATCTTAATCATTCATCCCCTCCCAGTGTATTGGATGGAAATGTAGATGATACTCTGTATGATCATCTTGAACAAGTGATGATAGAGGCTGAAAATGCAAAGAGAGACGCATTTCAGGAGGGAATCAGGCGTGGGAAAGCTGAAAAAGATGCCATTGATGCTATAAGGAGGGCTAAAGCATCAGAGGTCTTATATAATGAGGAGTTGAGACAAAGGAAAGAAATGGAAGAAGAACTGGCAAAAGAGAGACAAGAACTTGAAAAGATGAAGAAGCGACGAGATGAAGTCATGGAAGAATTACGGGCTGCCCTAGATCAGAAATCACTACTTGAGAGTCAAATTGCAGAGTCTGATCAGATGGTCATGAGCTTGGAGCAGAAGATTATCTCGGCTGTGGAACTTTTAAAAAAATACAAAAGAGAACGCGATGAGTTGCATGTGGAACGTGACAATGCACTTAGAGAAGCTGAGGAGCTGAGGGAAAAACAAGGAGAAGCCTCAAGTTCACACATACCTCAGTTCTTCTCTGAGTTCTCATTTGCAGAGATTGAAGAAGCAACTCAAAACTTTAACCCATCACTGAAGATTGGAGAAGGGGGTTATGGGAGCATTTTTAAAGGTTCCCTACGTCACACTCAGGTGGCTATAAAAACTTTAAATGCTCATAGTAAGCAAGGCCCCTCTGAGTTTCAGCAAGAGGTGGATGTATTGAGTAAGTTACGACACCAAAATCTTATCACGCTCATTGGTGCCTGCCCAGAAGGTTGGACTCTGATCTATGAGTATCTCCCAAATGGAAGTCTTGAAGATCGACTCAGCTGCAAGGACAATACTTCCCCTTTGTCATGGCAAACTCGAATACGAATTGCCACAGAGTTGTGCTCCGTCCTAATCTTTCTCCATTCCAGTAAACCTCACAGCATAGTGCATGGTGACTTAAAACCTGCCAATATTCTCCTTGATGCTAACTTTGTTAGCAAACTCAGTGACTTTGGAATCTGTCGGCTGTTGCCTCGTGGGGAGAGTTCAAGCAACGACACAACGATCTGTTGGAGAACTGACCCAAAGGGCACTATCCCATACATCGATCCTGAGTTCTTCTCATCAGGAGAGCTTACAACAAAGTCAGATGTTTACTCATTTGGAATTATACTGCTACAGTTGCTGACTGGGAAACAGGCTGTGAGGATAATAAAGGATGTGCAGGATGCATTAGATTCCGGAAAGTTGGAAATGCTCTTAGATCCCTTGGCTGGGGATTGGCCATTTGTACAGGCTGAACAGTTGGCTCGCTTGGCATTAAGGTGTTGTGAGATGAGCCGGAAGTTGAGGGCAGACCTTGTATCGGATGTCTGGAGGGTACTTGAACCCATGAGGGCTTCATGTGGTTGCTCATCATCATTCCGGTTGGGTACTGAAGAGCATTTCCAACCTCCTCCATATTTTATTTGTCCCATTTTCCAGGAAGTCATGCAAGATCCGCATGTTGCAGCAGACGGTTATACTTATGAAGCAGAAGCTTTGAAAGGATGGTTGGACAGCGGTCACGACACTTCACCGATGACAAATCTGAAGCTTGAACACAAGAATCTCGTCCCCAACCGCGCTCTCCGTTCTGCAATTCAGGAGTGGCTGCAACAGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box

Protein Analysis

901

Amino Acids

101.18

Weight (kDa)

5.99

Isoelectric Point (pI)

48.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	546 - 796	2.9e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	547 - 752	7e-39	Protein tyrosine and serine/threonine kinase
U-box	PF04564	831 - 899	4e-19	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45910 AT2G45910
fragaria_vesca	FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340
malus_domestica	MD01G1061400.v1.1 MD07G1136700.v1.1
prunus_persica	Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1
pyrus_communis	pycom01g09150 pycom07g13440
rosa_chinensis	RchiOBHm_Chr1g0355361 RchiOBHm_Chr6g0265291
rosa_laevigata	RLG00000028150
rosa_multiflora	Rmu_sc0002037.1_g000029 Rmu_sc0004128.1_g000003
rosa_roxburghii	Rroxscaffold_4G00300470
rosa_rugosa	Rorug01G0246800
rosa_samantha	Rh1AG259300 Rh1BG228900 Rh1CG243300 Rh1DG256800
rosa_wichuraiana	Rw1G022860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2008
AccB1I	GGYRCC	3 cut(s)	124, 621, 1805
AccBSI	CCGCTC	1 cut(s)	742
AccII	CGCG	3 cut(s)	84, 1482, 2661
AccIII	TCCGGA	1 cut(s)	2276
AclWI	GGATC	5 cut(s)	367, 494, 2138, 2294, 2528
AcoI	YGGCCR	1 cut(s)	2318
AcsI	RAATTY	1 cut(s)	646
AcuI	CTGAAG	4 cut(s)	879, 1658, 2493, 2648
AfaI	GTAC	5 cut(s)	216, 856, 2328, 2418, 2470
AfiI	CCNNNNNNNGG	5 cut(s)	29, 409, 2309, 2310, 2401
AhdI	GACNNNNNGTC	1 cut(s)	550
AjnI	CCWGG	2 cut(s)	635, 2517
AjuI	GAANNNNNNNTTGG	4 cut(s)	396, 428, 2573, 2605
AleI	CACNNNNGTG	1 cut(s)	1694
Alw21I	GWGCWC	2 cut(s)	342, 1940
Alw26I	GTCTC	4 cut(s)	404, 1155, 1258, 1297
Alw44I	GTGCAC	1 cut(s)	338
AlwI	GGATC	5 cut(s)	367, 494, 2138, 2294, 2528
AlwNI	CAGNNNCTG	2 cut(s)	1406, 2221
Aor13HI	TCCGGA	1 cut(s)	2276
AoxI	GGCC	2 cut(s)	1732, 2318
ApaLI	GTGCAC	1 cut(s)	338
ApeKI	GCWGC	7 cut(s)	509, 512, 679, 1361, 1875, 2546, 2689
ApoI	RAATTY	1 cut(s)	646
AspLEI	GCGC	1 cut(s)	2663
AspS9I	GGNCC	1 cut(s)	1733
AsuC2I	CCSGG	1 cut(s)	894
AsuHPI	GGTGA	5 cut(s)	248, 754, 986, 2000, 2604
AsuII	TTCGAA	1 cut(s)	888
AxyI	CCTNAGG	1 cut(s)	906
BaeGI	GKGCMC	3 cut(s)	342, 624, 2129
BaeI	ACNNNNGTAYC	2 cut(s)	654, 687
BalI	TGGCCA	1 cut(s)	2320
BanI	GGYRCC	3 cut(s)	124, 621, 1805
BauI	CACGAG	1 cut(s)	2070
Bbv12I	GWGCWC	2 cut(s)	342, 1940
BbvCI	CCTCAGC	2 cut(s)	1524, 1532
BbvI	GCAGC	7 cut(s)	521, 524, 666, 1348, 1862, 2558, 2676
BccI	CCATC	7 cut(s)	283, 647, 845, 1085, 1408, 1639, 2580
BcgI	CGANNNNNNTGC	2 cut(s)	1857, 1891
BciT130I	CCWGG	2 cut(s)	637, 2519
BclI	TGATCA	2 cut(s)	1117, 1408
BcnI	CCSGG	1 cut(s)	894
BcoDI	GTCTC	4 cut(s)	404, 1155, 1258, 1297
BfaI	CTAG	1 cut(s)	1367
BfmI	CTRYAG	2 cut(s)	6, 2213
BfuAI	ACCTGC	1 cut(s)	2008
BglII	AGATCT	1 cut(s)	1057
BisI	GCNGC	7 cut(s)	510, 513, 680, 1362, 1876, 2547, 2690
BlsI	GCNGC	7 cut(s)	511, 514, 681, 1363, 1877, 2548, 2691
Bme1390I	CCNGG	3 cut(s)	637, 894, 2519
BmeRI	GACNNNNNGTC	1 cut(s)	550
BmgT120I	GGNCC	1 cut(s)	1733
BmiI	GGNNCC	8 cut(s)	102, 126, 135, 623, 672, 1678, 1735, 1807
BmrFI	CCNGG	3 cut(s)	637, 894, 2519
BmrI	ACTGGG	2 cut(s)	1074, 2235
BmsI	GCATC	6 cut(s)	826, 1195, 1204, 1244, 2008, 2254
BmuI	ACTGGG	2 cut(s)	1074, 2235
BoxI	GACNNNNGTC	1 cut(s)	2598
BplI	GAGNNNNNCTC	6 cut(s)	744, 776, 1922, 1954, 2141, 2173
BpmI	CTGGAG	3 cut(s)	224, 246, 2431
Bpu10I	CCTNAGC	2 cut(s)	1524, 1532
Bpu14I	TTCGAA	1 cut(s)	888
BpuEI	CTTGAG	4 cut(s)	533, 759, 1406, 1540
BpuMI	CCSGG	1 cut(s)	894
Bsa29I	ATCGAT	1 cut(s)	2142
BsaBI	GATNNNNATC	2 cut(s)	724, 1062
BsaJI	CCNNGG	3 cut(s)	137, 636, 2304
BsaWI	WCCGGW	2 cut(s)	2276, 2460
BsaXI	ACNNNNNCTCC	2 cut(s)	78, 108
Bsc4I	CCNNNNNNNGG	5 cut(s)	29, 409, 2309, 2310, 2401
Bse118I	RCCGGY	2 cut(s)	103, 115
Bse1I	ACTGG	6 cut(s)	42, 970, 1080, 1296, 1962, 2230
Bse21I	CCTNAGG	1 cut(s)	906
Bse3DI	GCAATG	1 cut(s)	674
Bse8I	GATNNNNATC	2 cut(s)	724, 1062
BseAI	TCCGGA	1 cut(s)	2276
BseBI	CCWGG	2 cut(s)	637, 2519
BseCI	ATCGAT	1 cut(s)	2142
BseDI	CCNNGG	3 cut(s)	137, 636, 2304
BseGI	GGATG	8 cut(s)	147, 1070, 1096, 1765, 2260, 2269, 2410, 2591
BseJI	GATNNNNATC	2 cut(s)	724, 1062
BseLI	CCNNNNNNNGG	5 cut(s)	29, 409, 2309, 2310, 2401
BseMI	GCAATG	1 cut(s)	674
BseNI	ACTGG	6 cut(s)	42, 970, 1080, 1296, 1962, 2230
BseRI	GAGGAG	5 cut(s)	113, 946, 1271, 1541, 2481
BseSI	GKGCMC	3 cut(s)	342, 624, 2129
BseXI	GCAGC	7 cut(s)	521, 524, 666, 1348, 1862, 2558, 2676
BseYI	CCCAGC	2 cut(s)	304, 2309
BsgI	GTGCAG	1 cut(s)	2279
Bsh1236I	CGCG	3 cut(s)	84, 1482, 2661
BshFI	GGCC	2 cut(s)	1734, 2320
BshNI	GGYRCC	3 cut(s)	124, 621, 1805
BshVI	ATCGAT	1 cut(s)	2142
BsiHKAI	GWGCWC	2 cut(s)	342, 1940
BsiSI	CCGG	6 cut(s)	104, 116, 893, 2277, 2377, 2461
BslFI	GGGAC	4 cut(s)	307, 841, 2493, 2636
BslI	CCNNNNNNNGG	5 cut(s)	29, 409, 2309, 2310, 2401
BsmAI	GTCTC	4 cut(s)	404, 1155, 1258, 1297
BsmBI	CGTCTC	1 cut(s)	1155
BsmFI	GGGAC	4 cut(s)	307, 841, 2493, 2636
BsnI	GGCC	2 cut(s)	1734, 2320
Bsp119I	TTCGAA	1 cut(s)	888
Bsp1286I	GDGCHC	4 cut(s)	342, 624, 1940, 2129
Bsp13I	TCCGGA	1 cut(s)	2276
Bsp1407I	TGTACA	1 cut(s)	2326
BspANI	GGCC	2 cut(s)	1734, 2320
BspDI	ATCGAT	1 cut(s)	2142
BspEI	TCCGGA	1 cut(s)	2276
BspFNI	CGCG	3 cut(s)	84, 1482, 2661
BspHI	TCATGA	1 cut(s)	1419
BspLI	GGNNCC	8 cut(s)	102, 126, 135, 623, 672, 1678, 1735, 1807
BspMI	ACCTGC	1 cut(s)	2008
BspPI	GGATC	5 cut(s)	367, 494, 2138, 2294, 2528
BspQI	GCTCTTC	2 cut(s)	964, 2469
BspT104I	TTCGAA	1 cut(s)	888
BspT107I	GGYRCC	3 cut(s)	124, 621, 1805
BsrBI	CCGCTC	1 cut(s)	742
BsrDI	GCAATG	1 cut(s)	674
BsrFI	RCCGGY	2 cut(s)	103, 115
BsrGI	TGTACA	1 cut(s)	2326
BsrI	ACTGG	6 cut(s)	42, 970, 1080, 1296, 1962, 2230
BssAI	RCCGGY	2 cut(s)	103, 115
BssECI	CCNNGG	3 cut(s)	137, 636, 2304
BssSI	CACGAG	1 cut(s)	2070
BssT1I	CCWWGG	1 cut(s)	2304
Bst2BI	CACGAG	1 cut(s)	2070
Bst2UI	CCWGG	2 cut(s)	637, 2519
Bst4CI	ACNGT	4 cut(s)	446, 2217, 2340, 2555
Bst6I	CTCTTC	2 cut(s)	964, 2469
BstAUI	TGTACA	1 cut(s)	2326
BstBI	TTCGAA	1 cut(s)	888
BstC8I	GCNNGC	8 cut(s)	24, 82, 129, 264, 310, 796, 1810, 2347
BstDSI	CCRYGG	1 cut(s)	137
BstF5I	GGATG	8 cut(s)	147, 1070, 1096, 1765, 2260, 2269, 2410, 2591
BstFNI	CGCG	3 cut(s)	84, 1482, 2661
BstHHI	GCGC	1 cut(s)	2663
BstMAI	GTCTC	4 cut(s)	404, 1155, 1258, 1297
BstMWI	GCNNNNNNNGC	9 cut(s)	128, 133, 795, 1151, 1214, 2218, 2351, 2536, 2695
BstNI	CCWGG	2 cut(s)	637, 2519
BstNSI	RCATGY	3 cut(s)	903, 1496, 2543
BstPAI	GACNNNNGTC	1 cut(s)	2598
BstSCI	CCNGG	3 cut(s)	635, 892, 2517
BstSFI	CTRYAG	2 cut(s)	6, 2213
BstSLI	GKGCMC	3 cut(s)	342, 624, 2129
BstUI	CGCG	3 cut(s)	84, 1482, 2661
BstV1I	GCAGC	7 cut(s)	521, 524, 666, 1348, 1862, 2558, 2676
BstX2I	RGATCY	4 cut(s)	372, 1057, 2299, 2533
BstXI	CCANNNNNNTGG	2 cut(s)	1087, 1821
BstYI	RGATCY	4 cut(s)	372, 1057, 2299, 2533
Bsu15I	ATCGAT	1 cut(s)	2142
Bsu36I	CCTNAGG	1 cut(s)	906
BsuRI	GGCC	2 cut(s)	1734, 2320
BsuTUI	ATCGAT	1 cut(s)	2142
BtgI	CCRYGG	1 cut(s)	137
BtgZI	GCGATG	1 cut(s)	1497
BtsCI	GGATG	8 cut(s)	147, 1070, 1096, 1765, 2260, 2269, 2410, 2591
BtsI	GCAGTG	2 cut(s)	117, 174
BtsIMutI	CAGTG	5 cut(s)	117, 174, 1087, 1634, 2047
BveI	ACCTGC	1 cut(s)	2008
Cac8I	GCNNGC	8 cut(s)	24, 82, 129, 264, 310, 796, 1810, 2347
CaiI	CAGNNNCTG	2 cut(s)	1406, 2221
CciI	TCATGA	1 cut(s)	1419
CfoI	GCGC	1 cut(s)	2663
Cfr10I	RCCGGY	2 cut(s)	103, 115
Cfr13I	GGNCC	1 cut(s)	1733
ClaI	ATCGAT	1 cut(s)	2142
CseI	GACGC	1 cut(s)	1172
Csp6I	GTAC	5 cut(s)	215, 855, 2327, 2417, 2469
CviQI	GTAC	5 cut(s)	215, 855, 2327, 2417, 2469
DraI	TTTAAA	3 cut(s)	1461, 1672, 1713
DriI	GACNNNNNGTC	1 cut(s)	550
EaeI	YGGCCR	1 cut(s)	2318
Eam1104I	CTCTTC	2 cut(s)	964, 2469
Eam1105I	GACNNNNNGTC	1 cut(s)	550
EarI	CTCTTC	2 cut(s)	964, 2469
EciI	GGCGGA	2 cut(s)	61, 563
Eco130I	CCWWGG	1 cut(s)	2304
Eco57I	CTGAAG	4 cut(s)	879, 1658, 2493, 2648
Eco81I	CCTNAGG	1 cut(s)	906
EcoO109I	RGGNCCY	1 cut(s)	1733
EcoRII	CCWGG	2 cut(s)	635, 2517
EcoT14I	CCWWGG	1 cut(s)	2304
EcoT22I	ATGCAT	1 cut(s)	2269
ErhI	CCWWGG	1 cut(s)	2304
Esp3I	CGTCTC	1 cut(s)	1155
FaqI	GGGAC	4 cut(s)	307, 841, 2493, 2636
FauI	CCCGC	1 cut(s)	818
FbaI	TGATCA	2 cut(s)	1117, 1408
Fnu4HI	GCNGC	7 cut(s)	510, 513, 680, 1362, 1876, 2547, 2690
FokI	GGATG	8 cut(s)	154, 1057, 1103, 1772, 2267, 2276, 2417, 2598
Fsp4HI	GCNGC	7 cut(s)	510, 513, 680, 1362, 1876, 2547, 2690
FspBI	CTAG	1 cut(s)	1367
GlaI	GCGC	1 cut(s)	2662
GluI	GCNGC	7 cut(s)	510, 513, 680, 1362, 1876, 2547, 2690
GsaI	CCCAGC	2 cut(s)	308, 2313
GsuI	CTGGAG	3 cut(s)	224, 246, 2431
HaeIII	GGCC	2 cut(s)	1734, 2320
HapII	CCGG	6 cut(s)	104, 116, 893, 2277, 2377, 2461
HgaI	GACGC	1 cut(s)	1172
HhaI	GCGC	1 cut(s)	2663
Hin6I	GCGC	1 cut(s)	2661
HinP1I	GCGC	1 cut(s)	2661
HincII	GTYRAC	1 cut(s)	35
HindII	GTYRAC	1 cut(s)	35
HindIII	AAGCTT	2 cut(s)	2574, 2630
HpaII	CCGG	6 cut(s)	104, 116, 893, 2277, 2377, 2461
HphI	GGTGA	5 cut(s)	248, 754, 986, 2000, 2604
Hpy166II	GTNNAC	6 cut(s)	35, 256, 340, 1562, 1967, 2191
Hpy8I	GTNNAC	6 cut(s)	35, 256, 340, 1562, 1967, 2191
Hpy99I	CGWCG	1 cut(s)	1335
HpyAV	CCTTC	4 cut(s)	448, 635, 1643, 1811
HpyCH4III	ACNGT	4 cut(s)	446, 2217, 2340, 2555
HpyCH4IV	ACGT	3 cut(s)	180, 1501, 1684
HpyF10VI	GCNNNNNNNGC	9 cut(s)	128, 133, 795, 1151, 1214, 2218, 2351, 2536, 2695
HpySE526I	ACGT	3 cut(s)	180, 1501, 1684
HspAI	GCGC	1 cut(s)	2661
Kpn2I	TCCGGA	1 cut(s)	2276
Ksp22I	TGATCA	2 cut(s)	1117, 1408
LguI	GCTCTTC	2 cut(s)	964, 2469
LmnI	GCTCC	9 cut(s)	100, 133, 506, 739, 896, 1429, 1528, 1662, 1943
Lsp1109I	GCAGC	7 cut(s)	521, 524, 666, 1348, 1862, 2558, 2676
LweI	GCATC	6 cut(s)	826, 1195, 1204, 1244, 2008, 2254
MaeI	CTAG	1 cut(s)	1367
MaeII	ACGT	3 cut(s)	180, 1501, 1684
MaeIII	GTNAC	7 cut(s)	1028, 1502, 1685, 1773, 1988, 2042, 2600
MbiI	CCGCTC	1 cut(s)	742
MfeI	CAATTG	1 cut(s)	27
MflI	RGATCY	4 cut(s)	372, 1057, 2299, 2533
MhlI	GDGCHC	4 cut(s)	342, 624, 1940, 2129
MlsI	TGGCCA	1 cut(s)	2320
MluNI	TGGCCA	1 cut(s)	2320
MlyI	GAGTC	4 cut(s)	1399, 1412, 1819, 1863
MmeI	TCCRAC	5 cut(s)	1802, 2087, 2265, 2509, 2571
Mox20I	TGGCCA	1 cut(s)	2320
Mph1103I	ATGCAT	1 cut(s)	2269
MroI	TCCGGA	1 cut(s)	2276
MscI	TGGCCA	1 cut(s)	2320
MseI	TTAA	8 cut(s)	183, 1062, 1460, 1626, 1671, 1712, 1994, 2357
MslI	CAYNNNNRTG	2 cut(s)	1694, 2359
Msp20I	TGGCCA	1 cut(s)	2320
MspA1I	CMGCKG	3 cut(s)	131, 1875, 2598
MspI	CCGG	6 cut(s)	104, 116, 893, 2277, 2377, 2461
MspR9I	CCNGG	3 cut(s)	637, 894, 2519
MunI	CAATTG	1 cut(s)	27
MvaI	CCWGG	2 cut(s)	637, 2519
MvnI	CGCG	3 cut(s)	84, 1482, 2661
MwoI	GCNNNNNNNGC	9 cut(s)	128, 133, 795, 1151, 1214, 2218, 2351, 2536, 2695
NciI	CCSGG	1 cut(s)	894
NlaIV	GGNNCC	8 cut(s)	102, 126, 135, 623, 672, 1678, 1735, 1807
NmeAIII	GCCGAG	1 cut(s)	1424
NmuCI	GTSAC	5 cut(s)	1502, 1685, 1988, 2042, 2600
NsiI	ATGCAT	1 cut(s)	2269
NspI	RCATGY	3 cut(s)	903, 1496, 2543
NspV	TTCGAA	1 cut(s)	888
OliI	CACNNNNGTG	1 cut(s)	1694
PagI	TCATGA	1 cut(s)	1419
PciSI	GCTCTTC	2 cut(s)	964, 2469
PfeI	GAWTC	6 cut(s)	274, 988, 1179, 2052, 2273, 2644
PflFI	GACNNNGTC	1 cut(s)	687
PfoI	TCCNGGA	1 cut(s)	2517
PkrI	GCNGC	7 cut(s)	511, 514, 681, 1363, 1877, 2548, 2691
PleI	GAGTC	4 cut(s)	1398, 1411, 1819, 1863
PpsI	GAGTC	4 cut(s)	1398, 1411, 1819, 1863
PshAI	GACNNNNGTC	1 cut(s)	2598
Psp6I	CCWGG	2 cut(s)	635, 2517
PspFI	CCCAGC	2 cut(s)	304, 2309
PspGI	CCWGG	2 cut(s)	635, 2517
PspN4I	GGNNCC	8 cut(s)	102, 126, 135, 623, 672, 1678, 1735, 1807
PspPI	GGNCC	1 cut(s)	1733
PstNI	CAGNNNCTG	2 cut(s)	1406, 2221
PsuI	RGATCY	4 cut(s)	372, 1057, 2299, 2533
PsyI	GACNNNGTC	1 cut(s)	687
PvuII	CAGCTG	1 cut(s)	1875
RsaI	GTAC	5 cut(s)	216, 856, 2328, 2418, 2470
RsaNI	GTAC	5 cut(s)	215, 855, 2327, 2417, 2469
RseI	CAYNNNNRTG	2 cut(s)	1694, 2359
SapI	GCTCTTC	2 cut(s)	964, 2469
SaqAI	TTAA	8 cut(s)	183, 1062, 1460, 1626, 1671, 1712, 1994, 2357
SatI	GCNGC	7 cut(s)	510, 513, 680, 1362, 1876, 2547, 2690
Sau96I	GGNCC	1 cut(s)	1733
SchI	GAGTC	4 cut(s)	1399, 1412, 1819, 1863
ScrFI	CCNGG	3 cut(s)	637, 894, 2519
SduI	GDGCHC	4 cut(s)	342, 624, 1940, 2129
SfaNI	GCATC	6 cut(s)	826, 1195, 1204, 1244, 2008, 2254
SfcI	CTRYAG	2 cut(s)	6, 2213
SfuI	TTCGAA	1 cut(s)	888
SmiMI	CAYNNNNRTG	2 cut(s)	1694, 2359
SmlI	CTYRAG	4 cut(s)	548, 774, 1385, 1555
SmoI	CTYRAG	4 cut(s)	548, 774, 1385, 1555
SspI	AATATT	1 cut(s)	2008
SspMI	CTAG	1 cut(s)	1367
StyD4I	CCNGG	3 cut(s)	635, 892, 2517
StyI	CCWWGG	1 cut(s)	2304
TaaI	ACNGT	4 cut(s)	446, 2217, 2340, 2555
TaiI	ACGT	3 cut(s)	183, 1504, 1687
TaqI	TCGA	6 cut(s)	147, 751, 888, 1867, 1912, 2142
TatI	WGTACW	1 cut(s)	2326
TfiI	GAWTC	6 cut(s)	274, 988, 1179, 2052, 2273, 2644
Tru1I	TTAA	8 cut(s)	183, 1062, 1460, 1626, 1671, 1712, 1994, 2357
Tru9I	TTAA	8 cut(s)	183, 1062, 1460, 1626, 1671, 1712, 1994, 2357
TscAI	CASTG	5 cut(s)	117, 174, 1087, 1641, 2047
TseFI	GTSAC	5 cut(s)	1502, 1685, 1988, 2042, 2600
TseI	GCWGC	7 cut(s)	509, 512, 679, 1361, 1875, 2546, 2689
Tsp45I	GTSAC	5 cut(s)	1502, 1685, 1988, 2042, 2600
TspDTI	ATGAA	8 cut(s)	239, 380, 980, 1059, 1337, 1353, 2427, 2580
TspGWI	ACGGA	3 cut(s)	154, 1930, 2657
TspRI	CASTG	5 cut(s)	117, 174, 1087, 1641, 2047
Tth111I	GACNNNGTC	1 cut(s)	687
VneI	GTGCAC	1 cut(s)	338
XapI	RAATTY	1 cut(s)	646
XceI	RCATGY	3 cut(s)	903, 1496, 2543
XspI	CTAG	1 cut(s)	1367
Zsp2I	ATGCAT	1 cut(s)	2269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.