Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0005728

Physical Location & Seq

Genomic Coordinates Reverse (-)

20842821 .. 20848645

Gene Structure 5825 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00300470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2670 bp

ATGGCAGTTGTGAGTTCTGTGCCGGCAAAGATGCGACCGGCTGAGCCAATAAGGTATCCTGATATTTCAGATATGGGTAGTTTTGGAGGGATTGTGGTGGAGCCTGTGGCTCGGGTGATCCAAGATATGATATACGTTACAGTGGGAACTCCTGTGAAGGAGAGCAAATTGAATCTGATATGGACATTGCAGAACTCAGGAGGGAAGAGGGTTTGCATAGTGCATGTTCTCCAGCCTGCACAGATGATTCCAACGTCATTGGGAACCAAGTTCCCAGCAAGCCAAGTGAATCCTCAGGAAGTCAGAGCTTACCGTGAAAAGGAAAGGCAAGACATGCAGAAGTCGCTGGATGATTACCTTCATATTTGTCGTCAAATGGGGGTTCAAGCAGAAAAACTACGTATTGAAATGGACAGTATTGAGAAGGGAATTGTGGAACTCATCTCTCAGCATGGAATCAGAAGGCTTGTTATGGGAGCAGCAGCAGACAAGTCCCATTCAAGGAAAATGACAGACCTCAAGTCTAAAAAAGCCATATACGTGCGTGAACATGCACCTGTTTTCTGTCGCATACAATTTGTTTGCAAGGGGTACCTTATACACACCAGGGAAGCGAGTTCAGATGCAGTTGATGCACAGGTTGCATTTGCACCACCAAGGCCAAGCACTGACACTGAACAATCACCACACCATTTAAGATCACGATCTTCTGTGACACTTGGGCAGAATAATCGACCGTCACTCAGCAACCCAGCTCAAGATTTGTTCCGCCGGGCAAGATCGACAAAATCCGGCAAATATGGAGGAATCGCAACAGATGTTACTGAAGGGCTTTCAACTCCATCAAGCAGCAGGTTGGAAGCAGAAGGAAGTGCTGATGAATGGGATCAGGCATCCAGGAGGAGTACTTCTGGATATTCATCATGCTCTTCTGCATTGGGTGATTTACCTTTGATACAGTTTGGGAGGCCTGAGGGAAGTGAAAATGGGTCAAGAAACTCGAGTGCTCTTACTCATCTCAAAGATATTAATCTTTCATCCCCTCCCAGTGTATTGGATGGAAATGTAGACGATACTCTTTATGATCATCTTGAGCAAGCGATGACAGAGGCTGAAAATGCAAAGCGAGAAGCATTTGAAGAGGCAATTAGGCGTGGGAAAGCTGAAAAAGATGCCGTTGACGCTATACGAAGGGCCAAAGCATCAGAGTTCTTATACAATGAGGAGTTGAGACAAAGAAAAGAAATTGAGGACACAGTAGCAAGAGAAAGAGAAGAACTTGAAAAGATGAAGAAGCAACGAGATGAAGTCATGGAAGAATTCAGGGCCGCCTTAGAACAAAGATCACAACTCGAGAGCCAAATTGCTGAGTCTGATCAGCTGGTTCAGAGCTTGGAGCAGAAGATCATATCTGCTGTGGATCTCTTACAAAATTACAAAAGAGAACGGGATGAGTTGCATGTAGAACGTGACAATGCTCTTAGAGAAGCAGAGGAGCTAAGGAAAAGACAAGGAGAGGCCTCAAGCACACAGATGCCTCAGTTCTTCTCTGATTTTTCATTTCCCGAGATTGAGGAAGCAACTCAAAACTTTGACCCATCACTTAAGATTGGAGAAGGTGGATATGGTAGCATTTTCAAAGGTTTCCTACATCACTCTGAGGTGGCTATAAAAATGCTAAATGCTCATAGTTTGCAAGGCCCCTCTGAATTCCAGCAAGAGGTGGATATATTAAGTAAGCTGAGGCATACCAATCTTGTCACACTCATTGGTGCCTGCCCAGAAGCTTGGACTCTGATCTATGAGTATCTTCCAAATGGAAGCCTTGAAGATCGACTATGCTGCAAGGACAATACTCCCCCATTATCATGGCAAGCTCGAATACGCATTGCCACTGAGTTGTGTTCTGTCCTAATCTTTCTCCACTCCAGTAAACCACACAGCGTAGTACATGGTGATTTGAAACCCTCCAATATCCTCCTTGATGCTAACTTTGTTTGCAAACTTAGTGACTTTGGCATCAGTCGGCTCTTGTCTCGTGGTCAAGGTTCAAGCAACAACACAACACTCTGTTGCAGGACTGGTCCAAAAGGTACTTTCGCTTACATGGATCCTGAGTTCCTTTCATCAGGAGAGCTCACACCAAAGTCAGATGTGTATTCATTTGGAATTATACTCTTACGATTTTTAACCGGAAAACCAGCTTTGGGAATAGCAAAGGAAGTACAGTATGCATTAGATTCTGGAAGGTTGGAAACCCTCTTGGATCCTTTAGCGGGAGACTGGCCATTTGTACAGGCTGAACAGTTGGCTCGCTTAGCATTGAGGTGTTGTGAGATGAGCCGGAAGTGCAGGGCAGACCTTGTGTCAGATGTGTGGAGGGTACTTGAACCAATGAGGGCTTCATGTGGCTCCTCATCATCATTCCGGCTGGGTACTGAAGAGCACTTCCAACCTCCCTCATACTTCATCTGTCCCATTTTCCAGGAAGTCATGCAAGATCCACATGTTGCAGCAGATGGTTTCACTTATGAAGCAGAGGCTCTGAGGGGATGGCTGGACAGTGGTCATGACACTTCACCTATGACAAATCTTAAGCTCGAACACAAGAATCTCGTCCCCAACCACGCTCTTCGCTCTGCAATTCAGGAGTGGCTGCAACAGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box

Protein Analysis

889

Amino Acids

99.46

Weight (kDa)

5.91

Isoelectric Point (pI)

49.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	536 - 785	7.6e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	536 - 733	2.4e-37	Protein tyrosine and serine/threonine kinase
U-box	PF04564	820 - 889	1.5e-18	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45910 AT2G45910
fragaria_vesca	FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340
malus_domestica	MD01G1061400.v1.1 MD07G1136700.v1.1
prunus_persica	Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1
pyrus_communis	pycom01g09150 pycom07g13440
rosa_chinensis	RchiOBHm_Chr1g0355361 RchiOBHm_Chr6g0265291
rosa_laevigata	RLG00000028150
rosa_multiflora	Rmu_sc0002037.1_g000029 Rmu_sc0004128.1_g000003
rosa_roxburghii	Rroxscaffold_4G00300470
rosa_rugosa	Rorug01G0246800
rosa_samantha	Rh1AG259300 Rh1BG228900 Rh1CG243300 Rh1DG256800
rosa_wichuraiana	Rw1G022860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	843
Acc65I	GGTACC	1 cut(s)	591
AccB1I	GGYRCC	2 cut(s)	591, 1772
AccI	GTMKAC	1 cut(s)	1068
AciI	CCGC	3 cut(s)	769, 1329, 2276
AclWI	GGATC	8 cut(s)	112, 894, 1428, 2105, 2118, 2261, 2274, 2495
AcoI	YGGCCR	1 cut(s)	2285
AcsI	RAATTY	2 cut(s)	1319, 1709
AcuI	CTGAAG	2 cut(s)	846, 2460
AfaI	GTAC	8 cut(s)	593, 907, 1950, 2095, 2226, 2295, 2385, 2437
AfiI	CCNNNNNNNGG	5 cut(s)	319, 501, 1720, 2207, 2276
AflII	CTTAAG	2 cut(s)	1604, 2594
AflIII	ACRYGT	1 cut(s)	2506
AhdI	GACNNNNNGTC	2 cut(s)	520, 2365
AjnI	CCWGG	3 cut(s)	605, 896, 2484
AjuI	GAANNNNNNNTTGG	2 cut(s)	2386, 2418
Alw21I	GWGCWC	3 cut(s)	1009, 2139, 2448
Alw26I	GTCTC	3 cut(s)	1225, 2040, 2274
AlwI	GGATC	8 cut(s)	112, 894, 1428, 2105, 2118, 2261, 2274, 2495
AlwNI	CAGNNNCTG	1 cut(s)	1112
Ama87I	CYCGRG	4 cut(s)	111, 1000, 1352, 1565
AoxI	GGCC	7 cut(s)	659, 968, 1194, 1326, 1518, 1699, 2285
ApeKI	GCWGC	6 cut(s)	479, 482, 849, 1842, 2513, 2656
ApoI	RAATTY	2 cut(s)	1319, 1709
ArsI	GACNNNNNNTTYG	2 cut(s)	2273, 2305
AseI	ATTAAT	1 cut(s)	1029
Asp718I	GGTACC	1 cut(s)	591
AspS9I	GGNCC	4 cut(s)	1194, 1326, 1700, 2084
AsuC2I	CCSGG	1 cut(s)	773
AsuHPI	GGTGA	5 cut(s)	127, 675, 953, 1967, 2571
AvaI	CYCGRG	4 cut(s)	111, 1000, 1352, 1565
AvaII	GGWCC	1 cut(s)	2084
AxyI	CCTNAGG	2 cut(s)	294, 972
BalI	TGGCCA	1 cut(s)	2287
BamHI	GGATCC	2 cut(s)	2110, 2266
BanI	GGYRCC	2 cut(s)	591, 1772
BanII	GRGCYC	1 cut(s)	2139
BauI	CACGAG	1 cut(s)	2037
Bbv12I	GWGCWC	3 cut(s)	1009, 2139, 2448
BbvCI	CCTCAGC	1 cut(s)	1742
BbvI	GCAGC	6 cut(s)	491, 494, 861, 1829, 2525, 2643
BccI	CCATC	5 cut(s)	850, 1052, 1606, 2513, 2547
BceAI	ACGGC	1 cut(s)	1160
BcgI	CGANNNNNNTGC	4 cut(s)	713, 747, 1824, 1858
BciT130I	CCWGG	3 cut(s)	607, 898, 2486
BciVI	GTATCC	1 cut(s)	66
BclI	TGATCA	2 cut(s)	1084, 1375
BcnI	CCSGG	1 cut(s)	773
BcoDI	GTCTC	3 cut(s)	1225, 2040, 2274
BfrI	CTTAAG	2 cut(s)	1604, 2594
BfuAI	ACCTGC	1 cut(s)	843
BfuI	GTATCC	1 cut(s)	66
BisI	GCNGC	7 cut(s)	480, 483, 850, 1329, 1843, 2514, 2657
BlpI	GCTNAGC	2 cut(s)	42, 2317
BlsI	GCNGC	7 cut(s)	481, 484, 851, 1330, 1844, 2515, 2658
BmcAI	AGTACT	1 cut(s)	907
Bme1390I	CCNGG	4 cut(s)	607, 773, 898, 2486
Bme18I	GGWCC	1 cut(s)	2084
BmeRI	GACNNNNNGTC	2 cut(s)	520, 2365
BmeT110I	CYCGRG	4 cut(s)	111, 1000, 1352, 1565
BmgT120I	GGNCC	4 cut(s)	1194, 1326, 1700, 2084
BmiI	GGNNCC	8 cut(s)	102, 265, 593, 1702, 1774, 2112, 2268, 2413
BmrFI	CCNGG	4 cut(s)	607, 773, 898, 2486
BmrI	ACTGGG	1 cut(s)	1041
BmsI	GCATC	9 cut(s)	21, 613, 622, 902, 1162, 1211, 1524, 1975, 2028
BmuI	ACTGGG	1 cut(s)	1041
BoxI	GACNNNNGTC	1 cut(s)	2565
BpmI	CTGGAG	2 cut(s)	215, 1912
Bpu10I	CCTNAGC	2 cut(s)	1499, 1742
Bpu1102I	GCTNAGC	2 cut(s)	42, 2317
BpuEI	CTTGAG	4 cut(s)	503, 741, 1112, 1507
BpuMI	CCSGG	1 cut(s)	773
BsaAI	YACGTR	2 cut(s)	401, 541
BsaBI	GATNNNNATC	3 cut(s)	703, 919, 1029
BsaJI	CCNNGG	2 cut(s)	606, 656
BsaWI	WCCGGW	1 cut(s)	2192
BsaXI	ACNNNNNCTCC	2 cut(s)	78, 108
Bsc4I	CCNNNNNNNGG	5 cut(s)	319, 501, 1720, 2207, 2276
Bse118I	RCCGGY	2 cut(s)	22, 37
Bse1I	ACTGG	4 cut(s)	1047, 1929, 2086, 2288
Bse21I	CCTNAGG	2 cut(s)	294, 972
Bse3DI	GCAATG	2 cut(s)	185, 1887
Bse8I	GATNNNNATC	3 cut(s)	703, 919, 1029
BseBI	CCWGG	3 cut(s)	607, 898, 2486
BseDI	CCNNGG	2 cut(s)	606, 656
BseGI	GGATG	6 cut(s)	355, 893, 1037, 1063, 1456, 2558
BseJI	GATNNNNATC	3 cut(s)	703, 919, 1029
BseLI	CCNNNNNNNGG	5 cut(s)	319, 501, 1720, 2207, 2276
BseMI	GCAATG	2 cut(s)	185, 1887
BseNI	ACTGG	4 cut(s)	1047, 1929, 2086, 2288
BseRI	GAGGAG	4 cut(s)	916, 1238, 1508, 2404
BseXI	GCAGC	6 cut(s)	491, 494, 861, 1829, 2525, 2643
BseYI	CCCAGC	3 cut(s)	274, 751, 2431
BsgI	GTGCAG	2 cut(s)	222, 2371
Bsh1285I	CGRYCG	2 cut(s)	38, 737
BshFI	GGCC	7 cut(s)	661, 970, 1196, 1328, 1520, 1701, 2287
BshNI	GGYRCC	2 cut(s)	591, 1772
BsiEI	CGRYCG	2 cut(s)	38, 737
BsiHKAI	GWGCWC	3 cut(s)	1009, 2139, 2448
BsiHKCI	CYCGRG	4 cut(s)	111, 1000, 1352, 1565
BsiSI	CCGG	7 cut(s)	23, 38, 772, 792, 2193, 2344, 2428
BslFI	GGGAC	3 cut(s)	478, 2460, 2603
BslI	CCNNNNNNNGG	5 cut(s)	319, 501, 1720, 2207, 2276
BsmAI	GTCTC	3 cut(s)	1225, 2040, 2274
BsmFI	GGGAC	3 cut(s)	478, 2460, 2603
BsnI	GGCC	7 cut(s)	661, 970, 1196, 1328, 1520, 1701, 2287
BsoBI	CYCGRG	4 cut(s)	111, 1000, 1352, 1565
Bsp1286I	GDGCHC	3 cut(s)	1009, 2139, 2448
Bsp1407I	TGTACA	1 cut(s)	2293
Bsp1720I	GCTNAGC	2 cut(s)	42, 2317
BspACI	CCGC	3 cut(s)	769, 1329, 2276
BspANI	GGCC	7 cut(s)	661, 970, 1196, 1328, 1520, 1701, 2287
BspHI	TCATGA	1 cut(s)	2569
BspLI	GGNNCC	8 cut(s)	102, 265, 593, 1702, 1774, 2112, 2268, 2413
BspMI	ACCTGC	1 cut(s)	843
BspPI	GGATC	8 cut(s)	112, 894, 1428, 2105, 2118, 2261, 2274, 2495
BspQI	GCTCTTC	3 cut(s)	934, 2436, 2637
BspT107I	GGYRCC	2 cut(s)	591, 1772
BspTI	CTTAAG	2 cut(s)	1604, 2594
BsrDI	GCAATG	2 cut(s)	185, 1887
BsrFI	RCCGGY	2 cut(s)	22, 37
BsrGI	TGTACA	1 cut(s)	2293
BsrI	ACTGG	4 cut(s)	1047, 1929, 2086, 2288
BssAI	RCCGGY	2 cut(s)	22, 37
BssECI	CCNNGG	2 cut(s)	606, 656
BssSI	CACGAG	1 cut(s)	2037
BssT1I	CCWWGG	1 cut(s)	656
Bst2BI	CACGAG	1 cut(s)	2037
Bst2UI	CCWGG	3 cut(s)	607, 898, 2486
Bst4CI	ACNGT	9 cut(s)	142, 314, 416, 738, 960, 1258, 2229, 2307, 2564
Bst6I	CTCTTC	5 cut(s)	200, 934, 1134, 2436, 2637
BstAFI	CTTAAG	2 cut(s)	1604, 2594
BstAPI	GCANNNNNTGC	3 cut(s)	334, 632, 641
BstAUI	TGTACA	1 cut(s)	2293
BstBAI	YACGTR	2 cut(s)	401, 541
BstC8I	GCNNGC	7 cut(s)	24, 237, 280, 1098, 1777, 1875, 2314
BstF5I	GGATG	6 cut(s)	355, 893, 1037, 1063, 1456, 2558
BstMAI	GTCTC	3 cut(s)	1225, 2040, 2274
BstMCI	CGRYCG	2 cut(s)	38, 737
BstMWI	GCNNNNNNNGC	9 cut(s)	334, 343, 632, 641, 1118, 1181, 2318, 2349, 2662
BstNI	CCWGG	3 cut(s)	607, 898, 2486
BstNSI	RCATGY	5 cut(s)	227, 337, 554, 1463, 2510
BstPAI	GACNNNNGTC	1 cut(s)	2565
BstSCI	CCNGG	4 cut(s)	605, 771, 896, 2484
BstSNI	TACGTA	1 cut(s)	401
BstV1I	GCAGC	6 cut(s)	491, 494, 861, 1829, 2525, 2643
BstX2I	RGATCY	4 cut(s)	1420, 2110, 2266, 2500
BstXI	CCANNNNNNTGG	3 cut(s)	1054, 1788, 1869
BstYI	RGATCY	4 cut(s)	1420, 2110, 2266, 2500
Bsu36I	CCTNAGG	2 cut(s)	294, 972
BsuI	GTATCC	1 cut(s)	66
BsuRI	GGCC	7 cut(s)	661, 970, 1196, 1328, 1520, 1701, 2287
BtgZI	GCGATG	1 cut(s)	1115
BtsCI	GGATG	6 cut(s)	355, 893, 1037, 1063, 1456, 2558
BtsIMutI	CAGTG	7 cut(s)	147, 666, 672, 1054, 1893, 2569, 2665
BveI	ACCTGC	1 cut(s)	843
Cac8I	GCNNGC	7 cut(s)	24, 237, 280, 1098, 1777, 1875, 2314
CaiI	CAGNNNCTG	1 cut(s)	1112
CciI	TCATGA	1 cut(s)	2569
Cfr10I	RCCGGY	2 cut(s)	22, 37
Cfr13I	GGNCC	4 cut(s)	1194, 1326, 1700, 2084
CseI	GACGC	1 cut(s)	1190
Csp6I	GTAC	8 cut(s)	592, 906, 1949, 2094, 2225, 2294, 2384, 2436
CviQI	GTAC	8 cut(s)	592, 906, 1949, 2094, 2225, 2294, 2384, 2436
DriI	GACNNNNNGTC	2 cut(s)	520, 2365
EaeI	YGGCCR	1 cut(s)	2285
Eam1104I	CTCTTC	5 cut(s)	200, 934, 1134, 2436, 2637
Eam1105I	GACNNNNNGTC	2 cut(s)	520, 2365
EarI	CTCTTC	5 cut(s)	200, 934, 1134, 2436, 2637
EciI	GGCGGA	1 cut(s)	758
Ecl136II	GAGCTC	1 cut(s)	2137
Eco105I	TACGTA	1 cut(s)	401
Eco130I	CCWWGG	1 cut(s)	656
Eco147I	AGGCCT	2 cut(s)	970, 1520
Eco24I	GRGCYC	1 cut(s)	2139
Eco47I	GGWCC	1 cut(s)	2084
Eco53kI	GAGCTC	1 cut(s)	2137
Eco57I	CTGAAG	2 cut(s)	846, 2460
Eco81I	CCTNAGG	2 cut(s)	294, 972
Eco88I	CYCGRG	4 cut(s)	111, 1000, 1352, 1565
EcoICRI	GAGCTC	1 cut(s)	2137
EcoO109I	RGGNCCY	1 cut(s)	1700
EcoRI	GAATTC	2 cut(s)	1319, 1709
EcoRII	CCWGG	3 cut(s)	605, 896, 2484
EcoT14I	CCWWGG	1 cut(s)	656
EcoT22I	ATGCAT	1 cut(s)	2236
EcoT38I	GRGCYC	1 cut(s)	2139
ErhI	CCWWGG	1 cut(s)	656
FaqI	GGGAC	3 cut(s)	478, 2460, 2603
FauI	CCCGC	1 cut(s)	2269
FbaI	TGATCA	2 cut(s)	1084, 1375
FblI	GTMKAC	1 cut(s)	1068
Fnu4HI	GCNGC	7 cut(s)	480, 483, 850, 1329, 1843, 2514, 2657
FokI	GGATG	6 cut(s)	362, 880, 1024, 1070, 1463, 2565
FriOI	GRGCYC	1 cut(s)	2139
Fsp4HI	GCNGC	7 cut(s)	480, 483, 850, 1329, 1843, 2514, 2657
GluI	GCNGC	7 cut(s)	480, 483, 850, 1329, 1843, 2514, 2657
GsaI	CCCAGC	3 cut(s)	278, 755, 2435
GsuI	CTGGAG	2 cut(s)	215, 1912
HaeIII	GGCC	7 cut(s)	661, 970, 1196, 1328, 1520, 1701, 2287
HapII	CCGG	7 cut(s)	23, 38, 772, 792, 2193, 2344, 2428
HgaI	GACGC	1 cut(s)	1190
HincII	GTYRAC	1 cut(s)	1180
HindII	GTYRAC	1 cut(s)	1180
HindIII	AAGCTT	1 cut(s)	1785
HinfI	GANTC	9 cut(s)	172, 247, 289, 456, 807, 1370, 1792, 2240, 2611
HpaII	CCGG	7 cut(s)	23, 38, 772, 792, 2193, 2344, 2428
HphI	GGTGA	5 cut(s)	127, 675, 953, 1967, 2571
Hpy166II	GTNNAC	4 cut(s)	548, 1069, 1180, 1934
Hpy8I	GTNNAC	4 cut(s)	548, 1069, 1180, 1934
HpyAV	CCTTC	9 cut(s)	151, 368, 418, 456, 821, 860, 1185, 1610, 2241
HpyCH4III	ACNGT	9 cut(s)	142, 314, 416, 738, 960, 1258, 2229, 2307, 2564
HpyCH4IV	ACGT	5 cut(s)	135, 254, 400, 540, 1468
HpyF10VI	GCNNNNNNNGC	9 cut(s)	334, 343, 632, 641, 1118, 1181, 2318, 2349, 2662
HpySE526I	ACGT	5 cut(s)	135, 254, 400, 540, 1468
KpnI	GGTACC	1 cut(s)	595
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
Ksp22I	TGATCA	2 cut(s)	1084, 1375
LguI	GCTCTTC	3 cut(s)	934, 2436, 2637
LmnI	GCTCC	5 cut(s)	100, 476, 1396, 1495, 2417
Lsp1109I	GCAGC	6 cut(s)	491, 494, 861, 1829, 2525, 2643
LweI	GCATC	9 cut(s)	21, 613, 622, 902, 1162, 1211, 1524, 1975, 2028
MaeII	ACGT	5 cut(s)	135, 254, 400, 540, 1468
MaeIII	GTNAC	7 cut(s)	136, 712, 738, 820, 1469, 1759, 2009
MflI	RGATCY	4 cut(s)	1420, 2110, 2266, 2500
MhlI	GDGCHC	3 cut(s)	1009, 2139, 2448
MlsI	TGGCCA	1 cut(s)	2287
MluNI	TGGCCA	1 cut(s)	2287
MlyI	GAGTC	2 cut(s)	1379, 1786
MmeI	TCCRAC	4 cut(s)	275, 837, 2232, 2476
Mox20I	TGGCCA	1 cut(s)	2287
Mph1103I	ATGCAT	1 cut(s)	2236
MroNI	GCCGGC	1 cut(s)	22
MscI	TGGCCA	1 cut(s)	2287
MseI	TTAA	6 cut(s)	695, 1029, 1605, 1733, 2189, 2595
MslI	CAYNNNNRTG	4 cut(s)	539, 1532, 1867, 2326
Msp20I	TGGCCA	1 cut(s)	2287
MspA1I	CMGCKG	1 cut(s)	1381
MspCI	CTTAAG	2 cut(s)	1604, 2594
MspI	CCGG	7 cut(s)	23, 38, 772, 792, 2193, 2344, 2428
MspR9I	CCNGG	4 cut(s)	607, 773, 898, 2486
MvaI	CCWGG	3 cut(s)	607, 898, 2486
MwoI	GCNNNNNNNGC	9 cut(s)	334, 343, 632, 641, 1118, 1181, 2318, 2349, 2662
NaeI	GCCGGC	1 cut(s)	24
NciI	CCSGG	1 cut(s)	773
NgoMIV	GCCGGC	1 cut(s)	22
NlaIV	GGNNCC	8 cut(s)	102, 265, 593, 1702, 1774, 2112, 2268, 2413
NmuCI	GTSAC	5 cut(s)	712, 738, 1469, 1759, 2009
NsiI	ATGCAT	1 cut(s)	2236
NspI	RCATGY	5 cut(s)	227, 337, 554, 1463, 2510
PaeR7I	CTCGAG	2 cut(s)	1000, 1352
PagI	TCATGA	1 cut(s)	2569
PceI	AGGCCT	2 cut(s)	970, 1520
PciI	ACATGT	1 cut(s)	2506
PciSI	GCTCTTC	3 cut(s)	934, 2436, 2637
PdiI	GCCGGC	1 cut(s)	24
PfeI	GAWTC	7 cut(s)	172, 247, 289, 456, 807, 2240, 2611
PfoI	TCCNGGA	2 cut(s)	896, 2484
PkrI	GCNGC	7 cut(s)	481, 484, 851, 1330, 1844, 2515, 2658
PleI	GAGTC	2 cut(s)	1378, 1786
PpsI	GAGTC	2 cut(s)	1378, 1786
Ppu21I	YACGTR	2 cut(s)	401, 541
PscI	ACATGT	1 cut(s)	2506
PshAI	GACNNNNGTC	1 cut(s)	2565
PshBI	ATTAAT	1 cut(s)	1029
Psp124BI	GAGCTC	1 cut(s)	2139
Psp6I	CCWGG	3 cut(s)	605, 896, 2484
PspFI	CCCAGC	3 cut(s)	274, 751, 2431
PspGI	CCWGG	3 cut(s)	605, 896, 2484
PspN4I	GGNNCC	8 cut(s)	102, 265, 593, 1702, 1774, 2112, 2268, 2413
PspPI	GGNCC	4 cut(s)	1194, 1326, 1700, 2084
PspXI	VCTCGAGB	1 cut(s)	1000
PstNI	CAGNNNCTG	1 cut(s)	1112
PsuI	RGATCY	4 cut(s)	1420, 2110, 2266, 2500
PvuII	CAGCTG	1 cut(s)	1381
RsaI	GTAC	8 cut(s)	593, 907, 1950, 2095, 2226, 2295, 2385, 2437
RsaNI	GTAC	8 cut(s)	592, 906, 1949, 2094, 2225, 2294, 2384, 2436
RseI	CAYNNNNRTG	4 cut(s)	539, 1532, 1867, 2326
SacI	GAGCTC	1 cut(s)	2139
SapI	GCTCTTC	3 cut(s)	934, 2436, 2637
SaqAI	TTAA	6 cut(s)	695, 1029, 1605, 1733, 2189, 2595
SatI	GCNGC	7 cut(s)	480, 483, 850, 1329, 1843, 2514, 2657
Sau96I	GGNCC	4 cut(s)	1194, 1326, 1700, 2084
ScaI	AGTACT	1 cut(s)	907
SchI	GAGTC	2 cut(s)	1379, 1786
ScrFI	CCNGG	4 cut(s)	607, 773, 898, 2486
SduI	GDGCHC	3 cut(s)	1009, 2139, 2448
SfaNI	GCATC	9 cut(s)	21, 613, 622, 902, 1162, 1211, 1524, 1975, 2028
Sfr274I	CTCGAG	2 cut(s)	1000, 1352
SinI	GGWCC	1 cut(s)	2084
SlaI	CTCGAG	2 cut(s)	1000, 1352
SmiMI	CAYNNNNRTG	4 cut(s)	539, 1532, 1867, 2326
SmlI	CTYRAG	8 cut(s)	518, 756, 1000, 1091, 1352, 1522, 1604, 2594
SmoI	CTYRAG	8 cut(s)	518, 756, 1000, 1091, 1352, 1522, 1604, 2594
SnaBI	TACGTA	1 cut(s)	401
SseBI	AGGCCT	2 cut(s)	970, 1520
SsiI	CCGC	3 cut(s)	769, 1329, 2276
SstI	GAGCTC	1 cut(s)	2139
StuI	AGGCCT	2 cut(s)	970, 1520
StyD4I	CCNGG	4 cut(s)	605, 771, 896, 2484
StyI	CCWWGG	1 cut(s)	656
TaaI	ACNGT	9 cut(s)	142, 314, 416, 738, 960, 1258, 2229, 2307, 2564
TaiI	ACGT	5 cut(s)	138, 257, 403, 543, 1471
TaqI	TCGA	7 cut(s)	733, 782, 1001, 1353, 1834, 1879, 2601
TatI	WGTACW	4 cut(s)	905, 1948, 2224, 2293
TauI	GCSGC	1 cut(s)	1331
TfiI	GAWTC	7 cut(s)	172, 247, 289, 456, 807, 2240, 2611
Tru1I	TTAA	6 cut(s)	695, 1029, 1605, 1733, 2189, 2595
Tru9I	TTAA	6 cut(s)	695, 1029, 1605, 1733, 2189, 2595
TscAI	CASTG	6 cut(s)	147, 673, 679, 1054, 1900, 2569
TseFI	GTSAC	5 cut(s)	712, 738, 1469, 1759, 2009
TseI	GCWGC	6 cut(s)	479, 482, 849, 1842, 2513, 2656
Tsp45I	GTSAC	5 cut(s)	712, 738, 1469, 1759, 2009
TspRI	CASTG	6 cut(s)	147, 673, 679, 1054, 1900, 2569
Vha464I	CTTAAG	2 cut(s)	1604, 2594
VpaK11BI	GGWCC	1 cut(s)	2084
VspI	ATTAAT	1 cut(s)	1029
XapI	RAATTY	2 cut(s)	1319, 1709
XceI	RCATGY	5 cut(s)	227, 337, 554, 1463, 2510
XhoI	CTCGAG	2 cut(s)	1000, 1352
XmiI	GTMKAC	1 cut(s)	1068
ZrmI	AGTACT	1 cut(s)	907
Zsp2I	ATGCAT	1 cut(s)	2236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.