Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0005728

Physical Location & Seq

Genomic Coordinates Reverse (-)

19444191 .. 19449150

Gene Structure 4960 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000028150

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGGCAGTTGTGAGTTCTGTGCCGGCAAAGATGCGACCGGCTGAGCCAATAAGGTATCCTGATATTTCAGATATGGGTAGTTTTGGAGAGATTGTGGTGGAGCCTGTGGCTCGGGTGATCCAAGATATGATATATGTTACAGTGGGAACTCCTGTGAAGGAGAGCAAATTGAATCTGATATGGACATTGCAAAACTCAGGAGGGAAGAGGGTTTGCATAGTTCATGTTCTCCAGCCTGCACAGATGATTCCAACGTCATTGGGAACCAAGTTCCCAGCAAGCCAAGTGAATCCTCAGGAAGTCAGAGCTTACCGTGAAAAGGAAAGGCAAGACATGCAGAAGTTGCTGGATGATTACCTTCATATTTGTCGTCAAATGGGGGTTCGAGCAGAAAAACTACATATTGAAAGGGACAGTATTGAGAAGGGAATTGTGGAACTCATCTCTCAGCATGGAATCAGAAGGCTTGTTATGGGAGCAGCAGCGGACAAGTCCCATTCAAGGAAAATGACAGACCTCAAGTCTAAGAAAGCCGTATACGTGCGTGAACATGCACCTGGTTTCTGTCGCATACAATTTGTTTGCAAGGGATACCTTATACACACCAGGGAAGCGAGTTCAGATGCAGTTGATGCACAGGTGCCGCCACCAAGACCAAGCACCGACACTGAACAATCACCACACCATTTAAGATCACGATCTTCTGTGACACTTGGGCAGAATAAACAACCGTCACTCAGCAACCCAGCTCAAGATTTATTCCGCCGGGTAAGATCGACAAAATCCGGCAAATATGGAGGAATTGCAACAGATGTTACTGAAGGGCTTTCAACTCCATCAAGCAGCAGGTTGGAAGCAGAAGGGAGTGCTGATGAATGGGATCAGGCATCCAGGAGGAGTACTTCTGGATATTCATCATGCTCTTCTGCATTGGGTGATTTACCTTTGATACAGTTTGAGAGGACTGAGGGAAGTGAAAATGGGTCAAGAAACTCGAGTGCTCTTACTCATCTCAAAGATATTAATCGTTCATCCCCTCCCAGTGTATTGGATGGAAATGTAAACGATACTCTTTATGATCATCTTGAGCAAGCGATGACAGAGGCTGAAAATGCAAAGCGAGATGCATTTGAAGAGGCAATTAGGCGTGGGAAAGCTGAAAAAGACGCCATTGACGCTATACGAAGGGCCAAAGCATCAGAGTTCTTATACAATGAGGAATTGAGACAAAGAAAAGAAATTGAGGACACAGTAGCAAGAGAAAGAGAAGAACTTGAAGAGATGAAGAAGCAACGAGATGAAGTCATGGAAGAATTCAGGGCCGCCTTAGAACAAAGATCACAACTTGAGAGCCAAATTGCTGAGTCTGATCAGCTGGTTCAGCGCTTGGAGCAAAAGATCATATCTGCTGTGGATCTCTTACAAAATTACAAAAGAGAACGGGATGAGTTGCATGTAGAACGTGACAATGCTCTTAGAGAAGCAGAGGAGCTAAGGAAAAGACAAGGAGAGGCCTCAAGCACACAGATGCCTCAGTTCTTCTCTGATTTTTCATTTCCCGAGATTGAGGAAGCAACTCGAAACTTTGACCCATCACTTAAGATTGGAGAAGGTGGATATGGAAGCATTTTCAAAGGTTTCCTACGTCACACTGAGGTGGCTATAAAAATGCTAAATGCTCATAGTTTGCAAGGCCCCTCAGAATTCCAGCAAGAGGTGGATATATTAAGTAAGCTGAGGCATACCAATCTTGTCACACTCATTGGTGCCTGCCCAGAAGGTTGGACTCTGATCTATGAGTATCTTCCAAATGGAAGCCTTGAAGATCGACTAAGCTGCAAGGACAATACTCCCCCATTATCATGGCAAGCTCGAATACGCATTGCTACTGAGTTGTGTTCTGTCCTAATCTTTCTCCACTCCAGTAAACCACACAGCGTAGTACATGGTGATTTGAAACCCTCCAATATCCTCCTTGATGCTAACTTTGTTTGCAAACTTAGTGACTTTGGCATCAGTCGGCTCTTGTCTCGTGATCAAGGTTCAAGCGACAACACAACACTCTGTTGCTGGACTGGTCCAAAAGGTACTTTCGCTTACATGGATCCTGAGTTCCTTTCATCGGGAGAGCTTACACCAAAGTCAGATGTGTATTCGTTTGGAATTATACTCTTACGATTGTTAACCGGAAAACCAGCATTGGGAATAACAAAGGAAGTACAGTATGCATTAGATTCTGGAAAGTTGGAAACCCTCTTGGATCCTTTAGCGGGAGACTGGCCATTTGTACAGGCTGAACAGTTGGCTCGCTTAGCATTGAGGTGTTGTGAGATGAGCCGGAAGTGCAGGGCAGACCTTGTGTCAGATGTGTGGAGGGTACTTGAACCAATGAGGGCTTCATGTGGCTCCTTATCATCATTCCGGCTGGGTACTGAAGAGCACTTCCAAGCTCCCTCATACTTCATCTGTCCCATTTTCCAGGAAATCATGCAAGATCCACATGTTGCAGCAGATGGTTTCACTTATGAAGCAGAGGCTCTGAGGGGATGGCTGGACAGTGGTCATGACACTTCACCTATGACAAATCTTAAGCTCGAACACAAGAATCTCGTCCCCAACCACGCTCTTCGCTCTGCAATTCAGGAGTGGCTGCAACAGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box

Protein Analysis

888

Amino Acids

99.65

Weight (kDa)

5.87

Isoelectric Point (pI)

50.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	534 - 783	5.8e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	534 - 736	4e-38	Protein tyrosine and serine/threonine kinase
U-box	PF04564	817 - 887	1.9e-19	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45910 AT2G45910
fragaria_vesca	FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340 FvH4_7g14340
malus_domestica	MD01G1061400.v1.1 MD07G1136700.v1.1
prunus_persica	Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1 Prupe.2G170900_v2.0.a1
pyrus_communis	pycom01g09150 pycom07g13440
rosa_chinensis	RchiOBHm_Chr1g0355361 RchiOBHm_Chr6g0265291
rosa_laevigata	RLG00000028150
rosa_multiflora	Rmu_sc0002037.1_g000029 Rmu_sc0004128.1_g000003
rosa_roxburghii	Rroxscaffold_4G00300470
rosa_rugosa	Rorug01G0246800
rosa_samantha	Rh1AG259300 Rh1BG228900 Rh1CG243300 Rh1DG256800
rosa_wichuraiana	Rw1G022860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	837
AccB1I	GGYRCC	2 cut(s)	640, 1766
AccI	GTMKAC	1 cut(s)	537
AciI	CCGC	5 cut(s)	485, 644, 763, 1323, 2270
AclWI	GGATC	8 cut(s)	112, 888, 1422, 2099, 2112, 2255, 2268, 2489
AcoI	YGGCCR	1 cut(s)	2279
AcsI	RAATTY	2 cut(s)	1313, 1703
AcuI	CTGAAG	2 cut(s)	840, 2454
AcyI	GRCGYC	1 cut(s)	1167
AfaI	GTAC	7 cut(s)	901, 1944, 2089, 2220, 2289, 2379, 2431
AfeI	AGCGCT	1 cut(s)	1385
AfiI	CCNNNNNNNGG	6 cut(s)	319, 501, 1714, 2122, 2201, 2270
AflII	CTTAAG	2 cut(s)	1598, 2588
AflIII	ACRYGT	1 cut(s)	2500
AhdI	GACNNNNNGTC	2 cut(s)	520, 2359
AjnI	CCWGG	4 cut(s)	556, 605, 890, 2478
AjuI	GAANNNNNNNTTGG	2 cut(s)	2380, 2412
AleI	CACNNNNGTG	1 cut(s)	1655
AloI	GAACNNNNNNTCC	2 cut(s)	2096, 2128
Alw21I	GWGCWC	2 cut(s)	1003, 2442
Alw26I	GTCTC	3 cut(s)	1219, 2034, 2268
AlwI	GGATC	8 cut(s)	112, 888, 1422, 2099, 2112, 2255, 2268, 2489
AlwNI	CAGNNNCTG	1 cut(s)	1106
Ama87I	CYCGRG	3 cut(s)	111, 994, 1559
Aor51HI	AGCGCT	1 cut(s)	1385
AoxI	GGCC	5 cut(s)	1188, 1320, 1512, 1693, 2279
ApeKI	GCWGC	6 cut(s)	479, 482, 843, 1836, 2507, 2650
ApoI	RAATTY	2 cut(s)	1313, 1703
ArsI	GACNNNNNNTTYG	2 cut(s)	2267, 2299
AseI	ATTAAT	1 cut(s)	1023
AspLEI	GCGC	1 cut(s)	1386
AspS9I	GGNCC	4 cut(s)	1188, 1320, 1694, 2078
AsuC2I	CCSGG	1 cut(s)	767
AsuHPI	GGTGA	5 cut(s)	127, 669, 947, 1961, 2565
AvaI	CYCGRG	3 cut(s)	111, 994, 1559
AvaII	GGWCC	1 cut(s)	2078
AxyI	CCTNAGG	1 cut(s)	294
BalI	TGGCCA	1 cut(s)	2281
BamHI	GGATCC	2 cut(s)	2104, 2260
BanI	GGYRCC	2 cut(s)	640, 1766
BauI	CACGAG	1 cut(s)	2031
Bbv12I	GWGCWC	2 cut(s)	1003, 2442
BbvCI	CCTCAGC	1 cut(s)	1736
BbvI	GCAGC	6 cut(s)	491, 494, 855, 1823, 2519, 2637
BccI	CCATC	5 cut(s)	844, 1046, 1600, 2507, 2541
BceAI	ACGGC	1 cut(s)	518
BcgI	CGANNNNNNTGC	2 cut(s)	1818, 1852
BciT130I	CCWGG	4 cut(s)	558, 607, 892, 2480
BciVI	GTATCC	2 cut(s)	66, 584
BclI	TGATCA	3 cut(s)	1078, 1369, 2035
BcnI	CCSGG	1 cut(s)	767
BcoDI	GTCTC	3 cut(s)	1219, 2034, 2268
BfoI	RGCGCY	1 cut(s)	1387
BfrI	CTTAAG	2 cut(s)	1598, 2588
BfuAI	ACCTGC	1 cut(s)	837
BfuI	GTATCC	2 cut(s)	66, 584
BisI	GCNGC	8 cut(s)	480, 483, 644, 844, 1323, 1837, 2508, 2651
BlpI	GCTNAGC	2 cut(s)	42, 2311
BlsI	GCNGC	8 cut(s)	481, 484, 645, 845, 1324, 1838, 2509, 2652
BmcAI	AGTACT	1 cut(s)	901
Bme1390I	CCNGG	5 cut(s)	558, 607, 767, 892, 2480
Bme18I	GGWCC	1 cut(s)	2078
BmeRI	GACNNNNNGTC	2 cut(s)	520, 2359
BmeT110I	CYCGRG	3 cut(s)	111, 994, 1559
BmgT120I	GGNCC	4 cut(s)	1188, 1320, 1694, 2078
BmiI	GGNNCC	8 cut(s)	102, 265, 642, 1696, 1768, 2106, 2262, 2407
BmrFI	CCNGG	5 cut(s)	558, 607, 767, 892, 2480
BmrI	ACTGGG	1 cut(s)	1035
BmsI	GCATC	9 cut(s)	21, 613, 622, 896, 1114, 1205, 1518, 1969, 2022
BmuI	ACTGGG	1 cut(s)	1035
BoxI	GACNNNNGTC	1 cut(s)	2559
BpmI	CTGGAG	2 cut(s)	215, 1906
Bpu10I	CCTNAGC	2 cut(s)	1493, 1736
Bpu1102I	GCTNAGC	2 cut(s)	42, 2311
BpuEI	CTTGAG	5 cut(s)	503, 735, 1106, 1367, 1501
BpuMI	CCSGG	1 cut(s)	767
BsaAI	YACGTR	1 cut(s)	541
BsaBI	GATNNNNATC	3 cut(s)	697, 913, 1023
BsaHI	GRCGYC	1 cut(s)	1167
BsaJI	CCNNGG	1 cut(s)	606
BsaWI	WCCGGW	1 cut(s)	2186
BsaXI	ACNNNNNCTCC	2 cut(s)	78, 108
Bsc4I	CCNNNNNNNGG	6 cut(s)	319, 501, 1714, 2122, 2201, 2270
Bse118I	RCCGGY	2 cut(s)	22, 37
Bse1I	ACTGG	4 cut(s)	1041, 1923, 2080, 2282
Bse21I	CCTNAGG	1 cut(s)	294
Bse3DI	GCAATG	2 cut(s)	185, 1881
Bse8I	GATNNNNATC	3 cut(s)	697, 913, 1023
BseBI	CCWGG	4 cut(s)	558, 607, 892, 2480
BseDI	CCNNGG	1 cut(s)	606
BseGI	GGATG	6 cut(s)	355, 887, 1031, 1057, 1450, 2552
BseJI	GATNNNNATC	3 cut(s)	697, 913, 1023
BseLI	CCNNNNNNNGG	6 cut(s)	319, 501, 1714, 2122, 2201, 2270
BseMI	GCAATG	2 cut(s)	185, 1881
BseNI	ACTGG	4 cut(s)	1041, 1923, 2080, 2282
BseRI	GAGGAG	2 cut(s)	910, 1502
BseXI	GCAGC	6 cut(s)	491, 494, 855, 1823, 2519, 2637
BseYI	CCCAGC	3 cut(s)	274, 745, 2425
BsgI	GTGCAG	2 cut(s)	222, 2365
Bsh1285I	CGRYCG	1 cut(s)	38
BshFI	GGCC	5 cut(s)	1190, 1322, 1514, 1695, 2281
BshNI	GGYRCC	2 cut(s)	640, 1766
BsiEI	CGRYCG	1 cut(s)	38
BsiHKAI	GWGCWC	2 cut(s)	1003, 2442
BsiHKCI	CYCGRG	3 cut(s)	111, 994, 1559
BsiSI	CCGG	7 cut(s)	23, 38, 766, 786, 2187, 2338, 2422
BslFI	GGGAC	4 cut(s)	425, 478, 2454, 2597
BslI	CCNNNNNNNGG	6 cut(s)	319, 501, 1714, 2122, 2201, 2270
BsmAI	GTCTC	3 cut(s)	1219, 2034, 2268
BsmFI	GGGAC	4 cut(s)	425, 478, 2454, 2597
BsnI	GGCC	5 cut(s)	1190, 1322, 1514, 1695, 2281
BsoBI	CYCGRG	3 cut(s)	111, 994, 1559
Bsp1286I	GDGCHC	2 cut(s)	1003, 2442
Bsp1407I	TGTACA	1 cut(s)	2287
Bsp1720I	GCTNAGC	2 cut(s)	42, 2311
BspACI	CCGC	5 cut(s)	485, 644, 763, 1323, 2270
BspANI	GGCC	5 cut(s)	1190, 1322, 1514, 1695, 2281
BspHI	TCATGA	1 cut(s)	2563
BspLI	GGNNCC	8 cut(s)	102, 265, 642, 1696, 1768, 2106, 2262, 2407
BspMI	ACCTGC	1 cut(s)	837
BspPI	GGATC	8 cut(s)	112, 888, 1422, 2099, 2112, 2255, 2268, 2489
BspQI	GCTCTTC	3 cut(s)	928, 2430, 2631
BspT107I	GGYRCC	2 cut(s)	640, 1766
BspTI	CTTAAG	2 cut(s)	1598, 2588
BsrDI	GCAATG	2 cut(s)	185, 1881
BsrFI	RCCGGY	2 cut(s)	22, 37
BsrGI	TGTACA	1 cut(s)	2287
BsrI	ACTGG	4 cut(s)	1041, 1923, 2080, 2282
BssAI	RCCGGY	2 cut(s)	22, 37
BssECI	CCNNGG	1 cut(s)	606
BssNAI	GTATAC	1 cut(s)	538
BssNI	GRCGYC	1 cut(s)	1167
BssSI	CACGAG	1 cut(s)	2031
Bst1107I	GTATAC	1 cut(s)	538
Bst2BI	CACGAG	1 cut(s)	2031
Bst2UI	CCWGG	4 cut(s)	558, 607, 892, 2480
Bst4CI	ACNGT	9 cut(s)	142, 314, 416, 732, 954, 1252, 2223, 2301, 2558
Bst6I	CTCTTC	6 cut(s)	200, 928, 1128, 1272, 2430, 2631
BstACI	GRCGYC	1 cut(s)	1167
BstAFI	CTTAAG	2 cut(s)	1598, 2588
BstAPI	GCANNNNNTGC	3 cut(s)	334, 343, 632
BstAUI	TGTACA	1 cut(s)	2287
BstBAI	YACGTR	1 cut(s)	541
BstC8I	GCNNGC	7 cut(s)	24, 237, 280, 1092, 1771, 1869, 2308
BstF5I	GGATG	6 cut(s)	355, 887, 1031, 1057, 1450, 2552
BstH2I	RGCGCY	1 cut(s)	1387
BstHHI	GCGC	1 cut(s)	1386
BstMAI	GTCTC	3 cut(s)	1219, 2034, 2268
BstMCI	CGRYCG	1 cut(s)	38
BstNI	CCWGG	4 cut(s)	558, 607, 892, 2480
BstNSI	RCATGY	4 cut(s)	337, 554, 1457, 2504
BstPAI	GACNNNNGTC	1 cut(s)	2559
BstSCI	CCNGG	5 cut(s)	556, 605, 765, 890, 2478
BstV1I	GCAGC	6 cut(s)	491, 494, 855, 1823, 2519, 2637
BstX2I	RGATCY	4 cut(s)	1414, 2104, 2260, 2494
BstXI	CCANNNNNNTGG	3 cut(s)	1048, 1782, 1863
BstYI	RGATCY	4 cut(s)	1414, 2104, 2260, 2494
BstZ17I	GTATAC	1 cut(s)	538
Bsu36I	CCTNAGG	1 cut(s)	294
BsuI	GTATCC	2 cut(s)	66, 584
BsuRI	GGCC	5 cut(s)	1190, 1322, 1514, 1695, 2281
BtgZI	GCGATG	1 cut(s)	1109
BtsCI	GGATG	6 cut(s)	355, 887, 1031, 1057, 1450, 2552
BtsIMutI	CAGTG	6 cut(s)	147, 666, 1048, 1650, 2563, 2659
BveI	ACCTGC	1 cut(s)	837
Cac8I	GCNNGC	7 cut(s)	24, 237, 280, 1092, 1771, 1869, 2308
CaiI	CAGNNNCTG	1 cut(s)	1106
CciI	TCATGA	1 cut(s)	2563
CfoI	GCGC	1 cut(s)	1386
Cfr10I	RCCGGY	2 cut(s)	22, 37
Cfr13I	GGNCC	4 cut(s)	1188, 1320, 1694, 2078
CseI	GACGC	2 cut(s)	1175, 1184
CsiI	ACCWGGT	1 cut(s)	556
Csp6I	GTAC	7 cut(s)	900, 1943, 2088, 2219, 2288, 2378, 2430
CviQI	GTAC	7 cut(s)	900, 1943, 2088, 2219, 2288, 2378, 2430
DriI	GACNNNNNGTC	2 cut(s)	520, 2359
EaeI	YGGCCR	1 cut(s)	2279
Eam1104I	CTCTTC	6 cut(s)	200, 928, 1128, 1272, 2430, 2631
Eam1105I	GACNNNNNGTC	2 cut(s)	520, 2359
EarI	CTCTTC	6 cut(s)	200, 928, 1128, 1272, 2430, 2631
EciI	GGCGGA	1 cut(s)	752
Eco147I	AGGCCT	1 cut(s)	1514
Eco47I	GGWCC	1 cut(s)	2078
Eco47III	AGCGCT	1 cut(s)	1385
Eco57I	CTGAAG	2 cut(s)	840, 2454
Eco81I	CCTNAGG	1 cut(s)	294
Eco88I	CYCGRG	3 cut(s)	111, 994, 1559
EcoO109I	RGGNCCY	1 cut(s)	1694
EcoRI	GAATTC	2 cut(s)	1313, 1703
EcoRII	CCWGG	4 cut(s)	556, 605, 890, 2478
EcoT22I	ATGCAT	2 cut(s)	1129, 2230
FaqI	GGGAC	4 cut(s)	425, 478, 2454, 2597
FauI	CCCGC	1 cut(s)	2263
FbaI	TGATCA	3 cut(s)	1078, 1369, 2035
FblI	GTMKAC	1 cut(s)	537
Fnu4HI	GCNGC	8 cut(s)	480, 483, 644, 844, 1323, 1837, 2508, 2651
FokI	GGATG	6 cut(s)	362, 874, 1018, 1064, 1457, 2559
Fsp4HI	GCNGC	8 cut(s)	480, 483, 644, 844, 1323, 1837, 2508, 2651
GlaI	GCGC	1 cut(s)	1385
GluI	GCNGC	8 cut(s)	480, 483, 644, 844, 1323, 1837, 2508, 2651
GsaI	CCCAGC	3 cut(s)	278, 749, 2429
GsuI	CTGGAG	2 cut(s)	215, 1906
HaeII	RGCGCY	1 cut(s)	1387
HaeIII	GGCC	5 cut(s)	1190, 1322, 1514, 1695, 2281
HapII	CCGG	7 cut(s)	23, 38, 766, 786, 2187, 2338, 2422
HgaI	GACGC	2 cut(s)	1175, 1184
HhaI	GCGC	1 cut(s)	1386
Hin1I	GRCGYC	1 cut(s)	1167
Hin6I	GCGC	1 cut(s)	1384
HinP1I	GCGC	1 cut(s)	1384
HincII	GTYRAC	1 cut(s)	2184
HindII	GTYRAC	1 cut(s)	2184
HinfI	GANTC	8 cut(s)	172, 247, 289, 456, 1364, 1786, 2234, 2605
HpaI	GTTAAC	1 cut(s)	2184
HpaII	CCGG	7 cut(s)	23, 38, 766, 786, 2187, 2338, 2422
HphI	GGTGA	5 cut(s)	127, 669, 947, 1961, 2565
Hpy166II	GTNNAC	5 cut(s)	538, 548, 1063, 1928, 2184
Hpy8I	GTNNAC	5 cut(s)	538, 548, 1063, 1928, 2184
HpyAV	CCTTC	9 cut(s)	151, 368, 418, 456, 815, 854, 1179, 1604, 1772
HpyCH4III	ACNGT	9 cut(s)	142, 314, 416, 732, 954, 1252, 2223, 2301, 2558
HpyCH4IV	ACGT	4 cut(s)	254, 540, 1462, 1645
HpySE526I	ACGT	4 cut(s)	254, 540, 1462, 1645
Hsp92I	GRCGYC	1 cut(s)	1167
HspAI	GCGC	1 cut(s)	1384
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
Ksp22I	TGATCA	3 cut(s)	1078, 1369, 2035
KspAI	GTTAAC	1 cut(s)	2184
LguI	GCTCTTC	3 cut(s)	928, 2430, 2631
LmnI	GCTCC	6 cut(s)	100, 476, 1390, 1489, 2411, 2455
Lsp1109I	GCAGC	6 cut(s)	491, 494, 855, 1823, 2519, 2637
LweI	GCATC	9 cut(s)	21, 613, 622, 896, 1114, 1205, 1518, 1969, 2022
MabI	ACCWGGT	1 cut(s)	556
MaeII	ACGT	4 cut(s)	254, 540, 1462, 1645
MaeIII	GTNAC	8 cut(s)	136, 706, 732, 814, 1463, 1646, 1753, 2003
MflI	RGATCY	4 cut(s)	1414, 2104, 2260, 2494
MhlI	GDGCHC	2 cut(s)	1003, 2442
MlsI	TGGCCA	1 cut(s)	2281
MluNI	TGGCCA	1 cut(s)	2281
MlyI	GAGTC	2 cut(s)	1373, 1780
MmeI	TCCRAC	4 cut(s)	275, 831, 1763, 2226
Mox20I	TGGCCA	1 cut(s)	2281
Mph1103I	ATGCAT	2 cut(s)	1129, 2230
MroNI	GCCGGC	1 cut(s)	22
MscI	TGGCCA	1 cut(s)	2281
MseI	TTAA	6 cut(s)	689, 1023, 1599, 1727, 2183, 2589
MslI	CAYNNNNRTG	4 cut(s)	1526, 1655, 1861, 2320
Msp20I	TGGCCA	1 cut(s)	2281
MspA1I	CMGCKG	2 cut(s)	485, 1375
MspCI	CTTAAG	2 cut(s)	1598, 2588
MspI	CCGG	7 cut(s)	23, 38, 766, 786, 2187, 2338, 2422
MspR9I	CCNGG	5 cut(s)	558, 607, 767, 892, 2480
MvaI	CCWGG	4 cut(s)	558, 607, 892, 2480
NaeI	GCCGGC	1 cut(s)	24
NciI	CCSGG	1 cut(s)	767
NgoMIV	GCCGGC	1 cut(s)	22
NlaIV	GGNNCC	8 cut(s)	102, 265, 642, 1696, 1768, 2106, 2262, 2407
NmuCI	GTSAC	6 cut(s)	706, 732, 1463, 1646, 1753, 2003
NsiI	ATGCAT	2 cut(s)	1129, 2230
NspI	RCATGY	4 cut(s)	337, 554, 1457, 2504
OliI	CACNNNNGTG	1 cut(s)	1655
PaeR7I	CTCGAG	1 cut(s)	994
PagI	TCATGA	1 cut(s)	2563
PceI	AGGCCT	1 cut(s)	1514
PciI	ACATGT	1 cut(s)	2500
PciSI	GCTCTTC	3 cut(s)	928, 2430, 2631
PdiI	GCCGGC	1 cut(s)	24
PfeI	GAWTC	6 cut(s)	172, 247, 289, 456, 2234, 2605
PfoI	TCCNGGA	2 cut(s)	890, 2478
PkrI	GCNGC	8 cut(s)	481, 484, 645, 845, 1324, 1838, 2509, 2652
PleI	GAGTC	2 cut(s)	1372, 1780
PpsI	GAGTC	2 cut(s)	1372, 1780
Ppu21I	YACGTR	1 cut(s)	541
PscI	ACATGT	1 cut(s)	2500
PshAI	GACNNNNGTC	1 cut(s)	2559
PshBI	ATTAAT	1 cut(s)	1023
Psp6I	CCWGG	4 cut(s)	556, 605, 890, 2478
PspFI	CCCAGC	3 cut(s)	274, 745, 2425
PspGI	CCWGG	4 cut(s)	556, 605, 890, 2478
PspN4I	GGNNCC	8 cut(s)	102, 265, 642, 1696, 1768, 2106, 2262, 2407
PspPI	GGNCC	4 cut(s)	1188, 1320, 1694, 2078
PspXI	VCTCGAGB	1 cut(s)	994
PstNI	CAGNNNCTG	1 cut(s)	1106
PsuI	RGATCY	4 cut(s)	1414, 2104, 2260, 2494
PvuII	CAGCTG	1 cut(s)	1375
RsaI	GTAC	7 cut(s)	901, 1944, 2089, 2220, 2289, 2379, 2431
RsaNI	GTAC	7 cut(s)	900, 1943, 2088, 2219, 2288, 2378, 2430
RseI	CAYNNNNRTG	4 cut(s)	1526, 1655, 1861, 2320
SapI	GCTCTTC	3 cut(s)	928, 2430, 2631
SaqAI	TTAA	6 cut(s)	689, 1023, 1599, 1727, 2183, 2589
SatI	GCNGC	8 cut(s)	480, 483, 644, 844, 1323, 1837, 2508, 2651
Sau96I	GGNCC	4 cut(s)	1188, 1320, 1694, 2078
ScaI	AGTACT	1 cut(s)	901
SchI	GAGTC	2 cut(s)	1373, 1780
ScrFI	CCNGG	5 cut(s)	558, 607, 767, 892, 2480
SduI	GDGCHC	2 cut(s)	1003, 2442
SexAI	ACCWGGT	1 cut(s)	556
SfaNI	GCATC	9 cut(s)	21, 613, 622, 896, 1114, 1205, 1518, 1969, 2022
Sfr274I	CTCGAG	1 cut(s)	994
SinI	GGWCC	1 cut(s)	2078
SlaI	CTCGAG	1 cut(s)	994
SmiMI	CAYNNNNRTG	4 cut(s)	1526, 1655, 1861, 2320
SmlI	CTYRAG	8 cut(s)	518, 750, 994, 1085, 1346, 1516, 1598, 2588
SmoI	CTYRAG	8 cut(s)	518, 750, 994, 1085, 1346, 1516, 1598, 2588
SseBI	AGGCCT	1 cut(s)	1514
SsiI	CCGC	5 cut(s)	485, 644, 763, 1323, 2270
StuI	AGGCCT	1 cut(s)	1514
StyD4I	CCNGG	5 cut(s)	556, 605, 765, 890, 2478
TaaI	ACNGT	9 cut(s)	142, 314, 416, 732, 954, 1252, 2223, 2301, 2558
TaiI	ACGT	4 cut(s)	257, 543, 1465, 1648
TaqI	TCGA	7 cut(s)	385, 776, 995, 1579, 1828, 1873, 2595
TatI	WGTACW	4 cut(s)	899, 1942, 2218, 2287
TauI	GCSGC	2 cut(s)	646, 1325
TfiI	GAWTC	6 cut(s)	172, 247, 289, 456, 2234, 2605
Tru1I	TTAA	6 cut(s)	689, 1023, 1599, 1727, 2183, 2589
Tru9I	TTAA	6 cut(s)	689, 1023, 1599, 1727, 2183, 2589
TscAI	CASTG	5 cut(s)	147, 673, 1048, 1657, 2563
TseFI	GTSAC	6 cut(s)	706, 732, 1463, 1646, 1753, 2003
TseI	GCWGC	6 cut(s)	479, 482, 843, 1836, 2507, 2650
Tsp45I	GTSAC	6 cut(s)	706, 732, 1463, 1646, 1753, 2003
TspRI	CASTG	5 cut(s)	147, 673, 1048, 1657, 2563
Vha464I	CTTAAG	2 cut(s)	1598, 2588
VpaK11BI	GGWCC	1 cut(s)	2078
VspI	ATTAAT	1 cut(s)	1023
XapI	RAATTY	2 cut(s)	1313, 1703
XceI	RCATGY	4 cut(s)	337, 554, 1457, 2504
XhoI	CTCGAG	1 cut(s)	994
XmiI	GTMKAC	1 cut(s)	537
ZrmI	AGTACT	1 cut(s)	901
Zsp2I	ATGCAT	2 cut(s)	1129, 2230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.