Rorug01G0246800

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005728
Physical Location & Seq
Forward (+)
35363664 .. 35364286
623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0246800.1

Sequence Viewer

Length: 513 bp
ATGAATCAACCCGAAAGTTTTAGTGCCGCAATTGAAGGATGCATCGGGGTCTTCCATGTTGCTCATCCTATGCCTAACAAAGAACTTGATGAAGAATCAGTAACCAAAAAATCTGTAGAAGGTACCATAGGAATATTGAAACCCTGCCGAAATGCCAAGACTGTGAAGAGGGTTGTATACACTTCTAGTGCAGCAACTGTTTCTTATAATGGCAAAAACAAGGACATTGTCGATGAGAGTTCATGGAGTGATATTGAATATCATAGGTCCCTTAAATTATTTGGAACTTCATATGTGGCTGCCAAGACCAAAACAGAGCAAGTCCTGGAGTTTGCGGAAAAAAAATGGATTAGAACTTGTCACTTTAATCCCTCCATTCGTCCTTGGTGGATTTATCTGCAAAGATCTCCCTGGGTCAGTGGAGTTGATCCTATCCATGATTCTAGTAACATACGGTCACATGCTATATATGTCTCGCCTATGAAGTATTTCATGCCATTGAAGATAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr U-box

Protein Analysis

170

Amino Acids

19.34

Weight (kDa)

9.08

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 4 - 134 5.2e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
Acc65I GGTACC 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 177
AciI CCGC 2 cut(s) 27, 335
AclWI GGATC 1 cut(s) 422
AfaI GTAC 1 cut(s) 124
AgsI TTSAA 4 cut(s) 35, 139, 257, 502
AjnI CCWGG 2 cut(s) 324, 410
Alw26I GTCTC 1 cut(s) 478
AlwI GGATC 1 cut(s) 422
AlwNI CAGNNNCTG 1 cut(s) 197
ApeKI GCWGC 2 cut(s) 191, 299
Asp700I GAANNNNTTC 1 cut(s) 488
Asp718I GGTACC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 267
AvaII GGWCC 1 cut(s) 267
BanI GGYRCC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 43
BbvI GCAGC 2 cut(s) 203, 286
BciT130I CCWGG 2 cut(s) 326, 412
BcoDI GTCTC 1 cut(s) 478
BfaI CTAG 2 cut(s) 186, 444
BfmI CTRYAG 1 cut(s) 114
BglII AGATCT 1 cut(s) 404
BisI GCNGC 3 cut(s) 27, 192, 300
BlsI GCNGC 3 cut(s) 28, 193, 301
Bme1390I CCNGG 2 cut(s) 326, 412
Bme18I GGWCC 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 2 cut(s) 124, 269
BmrFI CCNGG 2 cut(s) 326, 412
BmsI GCATC 2 cut(s) 29, 51
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 347
BsaJI CCNNGG 3 cut(s) 383, 410, 411
BseBI CCWGG 2 cut(s) 326, 412
BseDI CCNNGG 3 cut(s) 383, 410, 411
BseGI GGATG 2 cut(s) 44, 64
BseXI GCAGC 2 cut(s) 203, 286
BsgI GTGCAG 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 122
BslFI GGGAC 1 cut(s) 253
BsmAI GTCTC 1 cut(s) 478
BsmFI GGGAC 1 cut(s) 253
Bsp143I GATC 2 cut(s) 404, 427
BspACI CCGC 2 cut(s) 27, 335
BspLI GGNNCC 2 cut(s) 124, 269
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 122
BssECI CCNNGG 3 cut(s) 383, 410, 411
BssMI GATC 2 cut(s) 404, 427
BssNAI GTATAC 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 383
Bst1107I GTATAC 1 cut(s) 178
Bst2UI CCWGG 2 cut(s) 326, 412
Bst4CI ACNGT 3 cut(s) 163, 199, 456
Bst6I CTCTTC 1 cut(s) 161
BstF5I GGATG 2 cut(s) 44, 64
BstKTI GATC 2 cut(s) 407, 430
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 2 cut(s) 404, 427
BstNI CCWGG 2 cut(s) 326, 412
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 2 cut(s) 324, 410
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 203, 286
BstV2I GAAGAC 1 cut(s) 43
BstX2I RGATCY 1 cut(s) 404
BstYI RGATCY 1 cut(s) 404
BstZ17I GTATAC 1 cut(s) 178
BtsCI GGATG 2 cut(s) 44, 64
BtsIMutI CAGTG 1 cut(s) 424
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 267
Csp6I GTAC 1 cut(s) 123
CviAII CATG 5 cut(s) 56, 243, 437, 461, 493
CviJI RGCY 1 cut(s) 299
CviKI_1 RGCY 1 cut(s) 299
CviQI GTAC 1 cut(s) 123
DpnI GATC 2 cut(s) 406, 429
DpnII GATC 2 cut(s) 404, 427
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 267
EcoRII CCWGG 2 cut(s) 324, 410
EcoT14I CCWWGG 1 cut(s) 383
EcoT22I ATGCAT 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 383
FaeI CATG 5 cut(s) 59, 246, 440, 464, 496
FaqI GGGAC 1 cut(s) 253
FatI CATG 5 cut(s) 55, 242, 436, 460, 492
FauNDI CATATG 1 cut(s) 292
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 27, 192, 300
FokI GGATG 2 cut(s) 51, 51
Fsp4HI GCNGC 3 cut(s) 27, 192, 300
FspBI CTAG 2 cut(s) 186, 444
GluI GCNGC 3 cut(s) 27, 192, 300
GsuI CTGGAG 1 cut(s) 347
Hin1II CATG 5 cut(s) 59, 246, 440, 464, 496
HinfI GANTC 3 cut(s) 4, 95, 440
Hpy166II GTNNAC 1 cut(s) 178
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 2 cut(s) 29, 113
HpyCH4III ACNGT 3 cut(s) 163, 199, 456
HpyCH4V TGCA 3 cut(s) 42, 191, 400
Hsp92II CATG 5 cut(s) 59, 246, 440, 464, 496
KpnI GGTACC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 404, 427
LpnPI CCDG 5 cut(s) 157, 311, 338, 397, 424
Lsp1109I GCAGC 2 cut(s) 203, 286
LweI GCATC 2 cut(s) 29, 51
MaeI CTAG 2 cut(s) 186, 444
MaeIII GTNAC 4 cut(s) 100, 359, 446, 456
MalI GATC 2 cut(s) 406, 429
MboI GATC 2 cut(s) 404, 427
MboII GAAGA 3 cut(s) 43, 104, 178
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 1 cut(s) 404
MluCI AATT 2 cut(s) 30, 275
MnlI CCTC 2 cut(s) 162, 382
Mph1103I ATGCAT 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 2 cut(s) 273, 366
MspR9I CCNGG 2 cut(s) 326, 412
MunI CAATTG 1 cut(s) 30
MvaI CCWGG 2 cut(s) 326, 412
NdeI CATATG 1 cut(s) 292
NdeII GATC 2 cut(s) 404, 427
NlaIII CATG 5 cut(s) 59, 246, 440, 464, 496
NlaIV GGNNCC 2 cut(s) 124, 269
NmuCI GTSAC 2 cut(s) 359, 456
NsiI ATGCAT 1 cut(s) 44
NspI RCATGY 1 cut(s) 464
PasI CCCWGGG 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 3 cut(s) 4, 95, 440
PflFI GACNNNGTC 1 cut(s) 227
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 3 cut(s) 28, 193, 301
PpuMI RGGWCCY 1 cut(s) 267
PsiI TTATAA 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 267
Psp6I CCWGG 2 cut(s) 324, 410
PspGI CCWGG 2 cut(s) 324, 410
PspN4I GGNNCC 2 cut(s) 124, 269
PspPI GGNCC 1 cut(s) 267
PspPPI RGGWCCY 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 404
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 1 cut(s) 124
RsaNI GTAC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 273, 366
SatI GCNGC 3 cut(s) 27, 192, 300
Sau3AI GATC 2 cut(s) 404, 427
Sau96I GGNCC 1 cut(s) 267
ScrFI CCNGG 2 cut(s) 326, 412
SetI ASST 2 cut(s) 124, 269
SfaNI GCATC 2 cut(s) 29, 51
SfcI CTRYAG 1 cut(s) 114
SinI GGWCC 1 cut(s) 267
Sse9I AATT 2 cut(s) 30, 275
SsiI CCGC 2 cut(s) 27, 335
SspI AATATT 1 cut(s) 135
SspMI CTAG 2 cut(s) 186, 444
StyD4I CCNGG 2 cut(s) 324, 410
StyI CCWWGG 1 cut(s) 383
TaaI ACNGT 3 cut(s) 163, 199, 456
TaqI TCGA 1 cut(s) 231
TasI AATT 2 cut(s) 30, 275
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 4, 95, 440
Tru1I TTAA 2 cut(s) 273, 366
Tru9I TTAA 2 cut(s) 273, 366
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 2 cut(s) 359, 456
TseI GCWGC 2 cut(s) 191, 299
Tsp45I GTSAC 2 cut(s) 359, 456
TspDTI ATGAA 6 cut(s) 17, 105, 231, 279, 481, 497
TspRI CASTG 1 cut(s) 424
Tth111I GACNNNGTC 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 267
XceI RCATGY 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 2 cut(s) 186, 444
Zsp2I ATGCAT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.